50th week of 2015 patent applcation highlights part 27 |
Patent application number | Title | Published |
20150353918 | METHOD AND MATERIALS FOR NUCLEIC ACIDS EXTRACTION AND PURIFICATION - Provided herein is materials and method relating to nucleic acids extraction and purification from biological samples. In particular, a pre-treatment buffer is used to facilitate the extraction and purification of nucleic acids from biological samples, more specifically, removing inhibitors and impurities from biological samples and resulting in a highly concentrated and purified nucleic acids preparation. | 2015-12-10 |
20150353919 | SAMPLE COLLECTION AND ANALYSIS DEVICES - Devices and methods for collecting, processing, and analyzing a sample. A sample collection module is configured for collecting, mixing diluting, and filtering a sample for analysis. A reaction cartridge is configured for processing a sample, mixing it with dried reagents, and conducting a chemical reaction for detecting target analytes. | 2015-12-10 |
20150353920 | METHODS FOR ISOLATING MICROVESICLES - The invention provides novel methods for isolating microvesicles from a biological sample and for extracting nucleic acids from the microvesicles. | 2015-12-10 |
20150353921 | METHOD OF ON-CHIP NUCLEIC ACID MOLECULE SYNTHESIS - A method of synthesizing a nucleic acid molecule, such as a gene, on a substrate or microchip is described. In particular, a method for synthesizing, amplifying, and assembling DNA oligonucleotides into a nucleic acid molecule or gene product, on a single substrate or microchip is described. Also described are a method of correcting a sequence error in a synthesized nucleic acid molecule, as well as a method for synthesizing and screening a library of codon variants to identify a nucleic acid molecule with an optimized level of protein expression. | 2015-12-10 |
20150353922 | METHODS AND SYSTEMS FOR IDENTIFYING PATIENT SPECIFIC DRIVER MUTATIONS - Methods for identifying patient specific driver mutations are provided. The methods provided identify specific patient derived markers associated with aberrant signal transduction pathways, in biological samples of a cancer patient. | 2015-12-10 |
20150353923 | Method for Identifying RNA Segments Bound by RNA-Binding Proteins or Ribonucleoprotein Complexes - The present invention relates to a method for identifying a binding site on an RNA transcript, wherein the binding site binds to one or more binding moieties. The method includes, among other things, introducing a photoreactive nucleoside into living cells wherein the living cells incorporate the photoreactive nucleoside into RNA transcripts during transcription thereby producing modified RNA transcripts; reverse transcribing the RNA of isolated cross-linked segments thereby generating cDNA transcripts with one mutation wherein the photoreactive nucleoside is transcribed to a mismatched deoxynucleoside; amplifying the cDNA transcripts thereby generating amplicons; and analyzing the sequences of the amplicons aligned against the reference sequence so as to identify the binding site, wherein the sequences of each amplicon having a mutation resulting from the introduction of the photoreactive nucleoside is considered to be a valid amplicon comprising at least a portion of a binding site on the RNA transcript. | 2015-12-10 |
20150353924 | GENERATION OF LIBRARY OF SOLUBLE RANDOM POLYPEPTIDES LINKED TO mRNA - Methods and compositions are provided for producing libraries of soluble random polypeptides. In the methods, the fraction of hydrophilic residues in the polypeptide is controlled so as to maintain the solubility of the polypeptide constructs. | 2015-12-10 |
20150353925 | TRANSPOSON END COMPOSITIONS AND METHODS FOR MODIFYING NUCLEIC ACIDS - The present invention provides methods, compositions and kits for using a transposase and a transposon end for generating extensive fragmentation and 5′-tagging of double-stranded target DNA in vitro, then using a DNA polymerase for generating 5′- and 3′-tagged single-stranded DNA fragments without performing a PCR amplification reaction, wherein the first tag on the 5′-ends exhibits the sequence of the transferred transposon end and optionally, an additional arbitrary sequence, and the second tag on the 3′-ends exhibits a different sequence from the sequence exhibited by the first tag. The method is useful for generating 5′- and 3′-tagged DNA fragments for use in a variety of processes, including processes for metagenomic analysis of DNA in environmental samples, copy number variation (CNV) analysis of DNA, and comparative genomic sequencing (CGS), including massively parallel DNA sequencing (so-called “next generation sequencing.) | 2015-12-10 |
20150353926 | POLYNUCLEOTIDE MODIFICATION ON SOLID SUPPORT - The present disclosure relates to the field of molecular biology and more specifically to methods for capturing and amplifying target polynucleotides on a solid surface. | 2015-12-10 |
20150353927 | Templates, Libraries, Kits and Methods for Generating Molecules - The present invention is directed to collections of templates for molecules such as RNA, as well as templates, devices, kits and methods for generating molecules from these collections. Through the use of various embodiments of the present invention one may efficiently and effectively obtain selected RNA molecules such as siRNA, shRNA, miRNA mimics and inhibitors, lncRNA, antisense RNA, aptamers, ribozymes, and sgRNA and sets of those molecules. | 2015-12-10 |
20150353928 | Genes Contributing to Survival of Cancer Cells - The invention relates to methods of inhibiting the growth or proliferation of a cell, the method comprising reducing the expression or activity of at least one gene in the cell selected from the group consisting of BLOC1S1, CDC2L1, CNOT1, CYR61, DDX54, EIF3I, FANCG, FBP1, IER2, KIF1A, LCK, NR2F1, PNRC1, POLR2A, POLR2B, POLR2C, PRPF6, PSMB4, PSMC5, PSMD1, PTK7, RPS2, SCNN1A, SF3A3, TAF2, TOB1, TSC22D4. | 2015-12-10 |
20150353929 | COMPOSITIONS AND METHODS FOR MODULATION OF SMN2 SPLICING - Disclosed herein are compounds, compositions and methods for modulating splicing of SMN2 mRNA in a cell, tissue or animal. Also provided are uses of disclosed compounds and compositions in the manufacture of a medicament for treatment of diseases and disorders, including spinal muscular atrophy. | 2015-12-10 |
20150353930 | METHODS OF TREATING A METABOLIC SYNDROME BY MODULATING HEAT SHOCK PROTEIN (HSP) 90-BETA - The invention provides HSP90β inhibitors comprising an antisense oligonucleotide targeting HSP90β, pharmaceutical compositions comprising said inhibitors and methods of treatment of a metabolic syndrome by administering said HSP90β inhibitors to a subject in need thereof. The antisense oligonucleotides may be targeted to skeletal muscle. | 2015-12-10 |
20150353931 | ANTISENSE OLIGONUCLEOTIDES FOR INDUCING EXON SKIPPING AND METHODS OF USE THEREOF - An antisense molecule capable of binding to a selected target site to induce exon skipping in the dystrophin gene, as set forth in SEQ ID NO: 1 to 214. | 2015-12-10 |
20150353932 | FUNCTIONAL LIGANDS TO TARGET MOLECULES - The present invention relates functional ligands to target molecules, particularly to functional nucleic acids and modifications thereof, and to methods for simultaneously generating, for example, numerous different functional biomolecules, particularly to methods for generating numerous different functional nucleic acids against multiple target molecules simultaneously. The present invention further relates to functional ligands which bind with affinity to target molecules. | 2015-12-10 |
20150353933 | APTAMER AGAINST MIDKINE AND APPLICATIONS THEREOF - The present invention provides an aptamer binding to midkine and capable of forming a potential secondary structure represented by the formula (I): | 2015-12-10 |
20150353934 | Lipid Formulated Compositions and Methods for Inhibiting Expression of a Gene from the Ebola Virus - The invention relates to lipid formulated double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of a gene from the Ebola virus. | 2015-12-10 |
20150353935 | MELK Regulation for the Treatment of Breast Cancer - Methods for inhibiting growth or proliferation of breast cancer cells are provided. The methods include administering to a subject in need thereof in an amount that is effective to inhibit growth or proliferation of the breast cancer cells a MELK inhibitor, wherein the breast cancer cells are estrogen receptor (ER) negative. In some aspects, the methods include administering to a subject in need thereof in an amount that is effective to inhibit growth or proliferation of the breast cancer cells a MELK inhibitor, a FoxM1 inhibitor or a MELK inhibitor and a FoxM1 inhibitor, wherein the breast cancer cells are estrogen receptor (ER) negative. Methods of treatment for breast cancer and methods of identifying patients having cancer that are likely to benefit from treatment with a MELK inhibitor, a FoxM1 inhibitor or a MELK inhibitor and a FoxM1 inhibitor are also provided. | 2015-12-10 |
20150353936 | ANTIGENE OLIGOMERS INHIBIT TRANSCRIPTION - Transcription of a gene in a mammalian cell is methylase-independently inhibited by contacting the cell with a nucleic acid oligomer of 12-28 bases complementary to a partially single-stranded target genomic sequence of the gene. | 2015-12-10 |
20150353937 | Compositions and Methods for Inhibiting Expression of a Target Gene - The present invention relates to a double-stranded ribonucleic acid (dsRNA) having a nucleotide sequence which is substantially identical to at least a part of a target gene and which is no more than 49, preferably less than 25, nucleotides in length, and which comprises a complementary (antisense) RNA strand having a 1 to 4 nucleotide overhang at the 3′-end and a blunt 5′-end. The invention further relates to a pharmaceutical composition comprising the dsRNA and a pharmaceutically acceptable carrier. The pharmaceutical compositions are useful for inhibiting the expression of a target gene, as well as for treating diseases caused by expression of the target gene, at low dosages (i.e., less than 5 milligrams, preferably less than 25 micrograms, per kg body weight per day). The invention also relates to methods for inhibiting the expression of a target gene, as well as methods for treating diseases caused by the expression of the gene. | 2015-12-10 |
20150353938 | CODON OPTIMIZED NUCLEIC ACID ENCODING A RETINITIS PIGMENTOSA GTPASE REGULATOR (RPGR) - This invention relates generally to a codon optimized nucleic acid encoding a retinitis pigmentosa GTPase regulator (RPGR) protein. The nucleic acid has enhanced stability during plasmid production relative to a wildtype cDNA encoding the RPGR protein. The invention also relates to expression cassettes, vectors, and host cells comprising the codon optimized nucleic acid. Methods for preparing a recombinant adeno-associated (rAAV) expression vector comprising the codon optimized nucleic acid sequence are also provided. The nucleic acids, expression cassettes, vectors, and host cells provided may be useful in the large scale production of rAAV expression vectors for gene therapy applications. | 2015-12-10 |
20150353939 | Growth Arrested Cells Useful for Producing Compounds - The present invention provides for a genetically modified host cell in a growth-arrested state producing an antibiotic. | 2015-12-10 |
20150353940 | VECTORS FOR USE IN AN INDUCIBLE COEXPRESSION SYSTEM - The present invention provides expression vectors for use in an inducible coexpression system, capable of controlled induction of expression of each gene product. | 2015-12-10 |
20150353941 | Genetically Modified Biological Cells - The present invention is based, in part, on our discovery of a way to configure expression cassettes so that the expression of a selectable marker protein, which is critical for the growth or survival of a cell, also results in the expression of a protein of interest in a biological cell. Accordingly, in one aspect, the invention features a genetically modified cell (e.g., a bacterial cell) that includes a chromosomally integrated or cytoplasmic expression cassette that includes a first nucleic acid sequence encoding a protein of interest an a second nucleic acid sequence encoding a selectable marker protein. The regulatory sequence (e.g., the sequence encoding a functional promoter) that drives expression of the required selectable marker protein also drives expression of the protein of interest. For that reason, we may refer to their expression as being “linked” or “functionally coupled.” | 2015-12-10 |
20150353942 | RECOMBINANT MICRO-ORGANISM FOR USE IN METHOD WITH INCREASED PRODUCT YIELD - The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism. | 2015-12-10 |
20150353943 | FUSION PROTEINS COMPRISING IMMUNOGLOBULIN CONSTANT DOMAIN-DERIVED SCAFFOLDS - This disclosure features fusion proteins comprising a base protein linked to or incorporated in a CH2 scaffold of IgG. The CH2 scaffold can derive from the macaque CH2 domain of IgG. The fusion proteins can effectively bind a single or multiple targets, and can be engineered to regulate effector functions as desired. The fusion proteins can have an increased serum half-life, solubility, stability, protease resistance, and/or expression as compared to the scaffolds alone and/or as compared to the base protein alone. This disclosure also features fusion proteins comprising a base protein, a CH2 scaffold and a discrete polyethylene glycol (dPEG) linked to the scaffold via a serine, tyrosine, cysteine, lysine, or a glycosylation site of the scaffold. This disclosure additionally features scaffolds linked to a discrete polyethylene glycol (dPEG) via a serine, tyrosine, cysteine, or lysine of the scaffolds or a glycosylation site of the scaffold. | 2015-12-10 |
20150353944 | METHOD FOR TRANSFORMING STRAMENOPILE - A method for transforming a stramenopile includes transferring a foreign gene into the stramenopile which is a microorganism belonging to the class Labyrinthula, more specifically, to a genus | 2015-12-10 |
20150353945 | NUCLEIC ACID SEQUENCES AND PEPTIDES/PROTEINS OF THE FT FAMILY PROVIDING FLOWER REPRESSING PROPERTIES IN TOBACCO AND TRANSGENIC PLANTS TRANSFORMED THEREWITH - The present invention is directed to nucleic acid sequences coding for a protein which, (1) under a respective promoter, is able to suppress or repress or delay flowering of a plant, and (2) includes the motive “NAPDIIDS” (SEQ ID NO: 10) or, in preferred cases, “VNAPDIIDS” SEQ ID NO: 67), with the exception of the nucleic acid of the gene StSP5G or a part thereof. Preferably, the nucleic acid sequence belongs phylogenetically to the FT-clade of the PEBP gene family, wherein the motive “(V)NAPDIIDS” (SEQ ID NO: 68) is in place of the “(V)YAPGW” motive_ (SEQ ID NO: 69) of the flowering promoting proteins AtFT and BvFT2. | 2015-12-10 |
20150353946 | METHOD FOR BREEDING BRASSICA RAPA PLANT HAVING SELF-COMPATIBILITY - The problem of providing a technology that converts a | 2015-12-10 |
20150353947 | TRANSGENIC PLANTS EXPRESSING CIVPS OR INTEIN MODIFIED PROTEINS AND RELATED METHOD - Transgenic plants that express CIVPS or intein modified proteins, compositions of matter comprising them, products of diverse applications made from the transgenic plants, methods to construct the transgenic plants containing CIVPS or intein modified genes, methods to express CIVPS or intein modified proteins in plants, and methods of using the transgenic plants. | 2015-12-10 |
20150353948 | Transcription Factor Which Regulates Flavonoid, Phenylpropanoid, Tyrosine, and Tryptophan Pathways - The invention provides plants having desirable levels of sugar release and/or lignin synthesis. The invention further provides methods of selecting plants with such desirable levels of sugar release and/or lignin synthesis; methods of genetically modifying plants to modulate lignin synthesis, sugar release, and modulating phenylalanine, tyrosine, tryptophan and flavonoid production; and uses of such plants. | 2015-12-10 |
20150353949 | METHOD FOR MODULATING PLANT ROOT ARCHITECTURE - The present invention provides a method for modulating or inhibiting modulation of the root architecture of a plant employing a new class of root architecture regulator (RAR) peptides, nucleotides encoding same, receptors therefor, binding agents thereof or agonists or antagonists thereof. Such methods encompass methods for increasing nutrient uptake by plants, increasing root nodule formation, promoting lateral root growth and development of plants, as well as methods for inhibiting root architecture modification resulting from root knot nematode action on plant roots. Also provided are RAR peptides, polynucleotides encoding RAR peptides and vectors and host cells comprising same, and plants obtained by methods of the invention or comprising RAR-encoding transgenes. | 2015-12-10 |
20150353950 | TRANSGENIC PLANTS - The invention relates to altering plant characteristics by manipulating plant genes. | 2015-12-10 |
20150353951 | SYNTHETIC GLYPHOSATE-RESISTANT GENE AND USE THEREOF - Disclosed in the present invention is a synthetic glyphosate-resistant gene and the use thereof. the gene provided in the present invention in one of following (a)-(c): (a) a DNA molecule having a nucleotide sequence shown as sequence 2 the sequence listing; (b) a DNA molecule having nucleotide sequence shown as positions 1-1335 of sequence 2 in the sequence listing; (c) a DNA molecule having a nucleotide sequence having an identity of at least 98% with the sequence 2 or the position 1-1335 of the sequence 2 in the sequence listing and encoding a protein shown as sequence 9. Experiment demonstrates that the transgenic maize with the synthetic glyphosate-resistant gene provided by the present invention has significantly increased G2-aroA protein expression and significantly improved tolerance to glyphosate compared with the transgenic maize with the prokaryote glyphosate-resistant gene G2-aroA. | 2015-12-10 |
20150353952 | SOYBEAN CULTIVAR S120071 - A soybean cultivar designated S120071 is disclosed. The invention relates to the seeds of soybean cultivar S120071, to the plants of soybean cultivar S120071, to the plant parts of soybean cultivar S120071, and to methods for producing progeny of soybean cultivar S120071. The invention also relates to methods for producing a soybean plant containing in its genetic material one or more transgenes and to the transgenic soybean plants and plant parts produced by those methods. The invention also relates to soybean cultivars or breeding cultivars, and plant parts derived from soybean cultivar S120071. The invention also relates to methods for producing other soybean cultivars, lines, or plant parts derived from soybean cultivar S120071, and to the soybean plants, varieties, and their parts derived from use of those methods. The invention further relates to hybrid soybean seeds, plants, and plant parts produced by crossing cultivar S120071 with another soybean cultivar. | 2015-12-10 |
20150353953 | HYBRID CANTALOUPE PLANT BRONCO - A novel hybrid cantaloupe plant, designated BRONCO is disclosed. The invention relates to the seeds of cantaloupe hybrid BRONCO, to the plants and plant parts of hybrid cantaloupe BRONCO, and to methods for producing a cantaloupe plant by crossing the hybrid cantaloupe BRONCO with itself or another cantaloupe plant. The invention further relates to methods for producing a cantaloupe plant containing in its genetic material one or more transgenes and to the transgenic plants produced by that method and to methods for producing other cantaloupe plants derived from the hybrid cantaloupe plant BRONCO. | 2015-12-10 |
20150353954 | REPELLENT COMPOSITIONS AND GENETIC APPROACHES FOR CONTROLLING HUANGLONGBING - The invention provides a method for controlling Huanglongbing (HLB) disease of citrus plants through expressing genes encoding synthases for sesquiterpenes such as β-caryophyllene, and α-copaene, and combinations thereof, in citrus plants. Methods of controlling HLB comprising applying at least one purified sesquiterpene, which repels | 2015-12-10 |
20150353955 | REPELLENT COMPOSITIONS AND GENETIC APPROACHES FOR CONTROLLING HUANGLONGBING - The invention provides a method for controlling Huanglongbing (HLB) disease of citrus plants through expressing genes encoding synthases for sesquiterpenes such as β-caryophyllene, and α-copaene, and combinations thereof, in citrus plants. Methods of controlling HLB comprising applying at least one purified sesquiterpene, which repels | 2015-12-10 |
20150353956 | FUNGAL RESISTANT PLANTS EXPRESSING EIN2 - The present invention relates to a method of increasing resistance against fungal pathogens of the family Phacopsoraceae in plants and/or plant cells. This is achieved by increasing the expression of an EIN2 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an EIN2 protein. | 2015-12-10 |
20150353957 | FUNGAL RESISTANT PLANTS EXPRESSING HCP7 - The present invention relates to a method of increasing resistance against fungal pathogens of the family Phacosporaceae in plants and/or plant cells. This is achieved by increasing the expression of an HCP7 protein or fragment thereof in a plant, plant part and/or plant cell in comparison to wild type plants, wild type plant parts and/or wild type plant cells. Furthermore, the invention relates to transgenic plants, plant parts, and/or plant cells having an increased resistance against fungal pathogens, in particular, pathogens of the family Phacopsoraceae, and to recombinant expression vectors comprising a sequence that is identical or homologous to a sequence encoding an HCP7 protein. | 2015-12-10 |
20150353958 | FERTILITY GENE AND USE THEREOF - The present disclosure relates to a fertility gene and the use thereof, and relates to the biotechnology field, particularly to a method of plant hybrid breeding including creation of a sterile line and preparation of hybrid seeds, more particularly to a fertility gene FL2, a mutant thereof and use thereof in hybrid breeding. | 2015-12-10 |
20150353959 | PEPTIDES FOR ENHANCING PROTEIN EXPRESSION - The present invention pertains to the field of recombinant protein production. Novel peptides derived from the extracellular region of a glycophorin protein are provided which enhance the expression rate of proteins or peptides of interest when expressed as fusion protein together with said novel peptides. | 2015-12-10 |
20150353960 | EFFICIENT PRODUCTION OF BIOFUELS FROM CELLS CARRYING A METABOLIC-BYPASS GENE CASSETTE - Increasing the production of a glycolytic intermediate and the production of an organic compound, such as ethanol, by a bacteria expressing a polyglutamine protein is achieved by preparing a feed stream; combining the bacteria and feed stream; fermenting the feed stream; cooling the feed stream during fermentation; recovering the organic compound; and concentrating the organic compound. The feed stream can be a waste stream from an ethanol production facility. The process can allow for a similar yield to the ethanol plant alone but with the use of less feed stock, such as corn, sugarcane, sorghum, cassava, switchgrass, and wood chips. | 2015-12-10 |
20150353961 | Designer Photoautotrophic and Hydrogenotrophic Production of Alcohols and Biodiesel - Designer Calvin-cycle-channeled and hydrogenotrophic biofuel-production pathways, the associated designer genes and designer transgenic organisms for autotrophic production of alcohols and biodiesel from carbon dioxide, hydrogen, and/or water are disclosed. The alcohols include methanol, ethanol, propanol, 1-butanol, 2-methyl-1-butanol, isobutanol, 3-methyl-1-butanol, 1-hexanol, 1-octanol, 1-pentanol, 1-heptanol, 3-methyl-1-pentanol, 4-methyl-1-hexanol, 5-methyl-1-heptanol, 4-methyl-1-pentanol, 5-methyl-1-hexanol, and 6-methyl-1-heptanol. The designer autotrophic organisms such as designer transgenic oxyphotobacteria and algae comprise designer Calvin-cycle-channeled and hydrogenotrophic pathway gene(s) and biosafety-guarding technology for enhanced autotrophic production of alcohols and biodiesel from carbon dioxide and water; wherein the designer transgenic cells in their mass liquid culture can inducibly self-flocculate for enhanced harvesting of their biomass upon the expression of the designer cell surface-linked positively charged polypeptides. | 2015-12-10 |
20150353962 | METHOD OF MANUFACTURING 1,4-BUTANEDIOL AND MICROBE - A method of manufacturing 1,4-butanediol, using a microbe and/or a culture thereof, by an enzyme reaction system that uses acyl-CoA reductase, via 3-hydroxybutyryl-CoA, crotonyl-CoA and 4-hydroxybutyryl CoA in this order, wherein the reactivity of the acyl-CoA reductase to 4-hydroxybutyryl CoA is greater than or equal to 0.05 times of the reactivity of the acyl-CoA reductase to 3-hydroxybutyryl-CoA. | 2015-12-10 |
20150353963 | PROCESS FOR PREPARING AN ALPHA, OMEGA-ALKANEDIOL - The invention relates to a process for preparing an α,ω-alkanediol comprising the steps of a) reacting an alkanoic acid with an alkanol to give an ester, b) oxidizing at least one terminal carbon atom of the ester by contacting with a whole-cell catalyst, which expresses an alkane hydroxylase, in aqueous solution and in the presence of molecular oxygen, to give an oxidized ester, c) hydrogenating the oxidized ester to form the alkanediol and alkanol, and d) removing the alkanol by distillation, forming a reaction mixture depleted with respect to the alkanol, and recycling the alkanol in step b). | 2015-12-10 |
20150353964 | MUTANTS HAVING CAPABILITY TO PRODUCE 1, 4-BUTANEDIOL AND METHOD FOR PREPARING 1, 4-BUTANEDIOL USING THE SAME - A mutant capable of producing 1,4-butanediol and a method of preparing 1,4-butanediol using the same are provided. The mutant microorganism is prepared by introducing and amplifying genes encoding enzymes converting succinate into 4-hydroxybutyrate and 4-hydroxybutyrate into 1,4-butanediol in a microorganism capable of producing succinate. The method includes culturing the mutant in a medium containing carbohydrate and obtaining 1,4-butanediol from the culture. Thus, 1,4-butanediol, which is essential in chemical industry, can be prepared in a biological process. | 2015-12-10 |
20150353965 | CARBON CAPTURE IN FERMENTATION - The invention relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO. The methods of the invention include converting CO to one or more products including alcohols and/or acids and optionally capturing CO2 to improve overall carbon capture. In certain aspects, the invention relates to processes for producing alcohols, particularly ethanol, from industrial waste streams, particularly steel mill off-gas. | 2015-12-10 |
20150353966 | BIOLOGICAL METHODS FOR PREPARING A FATTY DICARBOXYLIC ACID - The technology relates in part to biological methods for producing a fatty dicarboxylic acid and engineered microorganisms capable of such production. Provided are engineered microorganisms capable of producing fatty dicarboxylic acids and products expressed by such microorganisms. Also provided are biological methods for producing fatty dicarboxylic acids. | 2015-12-10 |
20150353967 | METHOD FOR INCREASED PRODUCTIVITY OF POLYHYDROXYALKANOATES (PHAS) IN FED-BATCH PROCESSES FOR BIOMASS DERIVED FROM THE TREATMENT OF WASTEWATER - This disclosure relates to methods for producing a PHA-rich-biomass from open mixed cultures. Mixed liquor containing biomass is directed into a fed-batch reactor. The reactor includes at least one biomass stimulating zone and at least one biomass maintenance zone. A feed containing RBCOD, bioavailable N, and bioavailable P is directed into the fed-batch reactor. The respiration rate of at least a portion of the biomass is intermittently and repeatedly stimulated in the stimulating zone by directing the feed into the fed-batch reactor and exposing the biomass to a relatively high concentration of RBCOD. The biomass is then transferred to the maintenance zone, where the biomass is exposed to a relatively low concentration of RBCOD. Thereafter, the biomass is circulated back and forth between the stimulating zone and the maintenance zone. Throughout the methods, the concentration of N and/or P relative to the RBCOD in the feed is controlled by controlling the ratio of N to RBCOD and/or the ration of P to RBCOD in the feed. | 2015-12-10 |
20150353968 | Production of Fatty Acid Alkyl Esters - A method for producing fatty acid alkyl esters, wherein a solution comprising triglyceride, alcohol, water, and glycerol is contacted with a lipolytic enzyme. | 2015-12-10 |
20150353969 | METHOD FOR IMPROVING YIELD OF ENZYMATIC PREPARATION OF BIODIESEL FROM GREASES - Provided in the present invention is a method for improving the yield of enzymatic preparation of biodiesel from greases, comprising: during enzymatic reaction of short-chain alcohols with greases to prepare biodiesel, introducing online dehydration steps, which lead the volatile gases in the enzyme reactor to enter into the low-temperature absorption tank, and the dehydrated gases flow back into the enzyme reactor. The formation of gas-circulation can constantly bring the moisture inside the enzyme reactor out. | 2015-12-10 |
20150353970 | ENZYMATIC TRANSESTERIFICATION/ESTERIFICATION PROCESSING SYSTEMS AND PROCESSES EMPLOYING LIPASES IMMOBILZED ON HYDROPHOBIC RESINS - Disclosed are processing systems and processes for carrying out enzymatic batchwise or continuous process for the production of fatty acid alkyl esters for use in the biofuels, food, cosmetics, pharmaceuticals and detergents industries. | 2015-12-10 |
20150353971 | BIOREFINERY SYSTEM, METHODS AND COMPOSITIONS THEREOF - The present disclosure relates to bioengineering approaches for producing biofuel and, in particular, to the use of a C | 2015-12-10 |
20150353972 | MICROORGANISM PRODUCING DOCOSAHEXAENOIC ACID AND UTILIZATION THEREOF - According to the present invention, a microorganism belonging to the genus | 2015-12-10 |
20150353973 | METHOD FOR THE FERMENTATIVE PRODUCTION OF L-AMINO ACIDS USING IMPROVED STRAINS OF THE ENTEROBACTERIACEAE FAMILY - The present invention relates to a process for the fermentative production of L-amino acids using microorganisms of the Enterobacteriaceae family, which harbour an attenuated proP gene, to the microorganisms suitable for said production and to polynucleotides coding for variants of the ProP transporter. | 2015-12-10 |
20150353974 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, microbial, and municipal waste biomass) is processed to produce useful products, such as food products and amino acids. | 2015-12-10 |
20150353975 | MICROORGANISMS AND METHODS FOR THE PRODUCTION OF CAPROLACTONE - The invention provides non-naturally occurring microbial organisms containing caprolactone pathways having at least one exogenous nucleic acid encoding a butadiene pathway enzyme expressed in a sufficient amount to produce caprolactone. The invention additionally provides methods of using such microbial organisms to produce caprolactone by culturing a non-naturally occurring microbial organism containing caprolactone pathways as described herein under conditions and for a sufficient period of time to produce caprolactone. | 2015-12-10 |
20150353976 | ENGINEERED BIOCATALYSTS USEFUL FOR CARBAPENEM SYNTHESIS - The present disclosure provides engineered pNB esterase polypeptides useful for the synthesis of the carbapenem antibiotic, imipenem. The disclosure also provides polynucleotides encoding the engineered pNB esterases, host cells capable of expressing the engineered pNB esterases, and methods of using the engineered pNB esterases in the production of imipenem. | 2015-12-10 |
20150353977 | METHOD FOR PREPARING FERMENTABLE SUGAR FROM WOOD-BASED BIOMASS - The present invention relates to a method for preparing, from wood-based biomass, a high concentration of fermentable sugar which can be effectively used in culturing various industrial fermented bacteria. According to the method of the present invention, biomass can be extracted by hot water prior to a pre-treatment so as to remove extractible substances such as mineral salts to thus minimize the content of impurities in raw materials for an enzymatic saccharification. The biomass from which substances extractible by hot water are removed is pre-treated in the condition where xylan yield rate is maximized, thus achieving maximum inhibition of the generation of over-decomposed products of sugar. Subsequently, fermentable sugar for culturing various industrial fermented bacteria can be prepared in an inexpensive manner by only concentrating, using a separator membrane, the sugar solution obtained by an enzymatic saccharification of the pre-treated solid content obtained by a solid-liquid separation without washing the solid content with water. | 2015-12-10 |
20150353978 | METHOD FOR PRODUCING FRUCTOSE - A method for the enzymatic production of D-fructose from D-glucose in a one-pot synthesis, wherein D-glucose is oxidized enzymatically to D-glucosone and D-glucosone is reduced enzymatically to D-fructose and the use of the D-fructose produced in this way for the production of furan derivatives. | 2015-12-10 |
20150353979 | Preparation Of Malto-Oligosaccharides - Disclosed is a method for preparing a mixture of malto-oligosaccharides. Generally, a dry-milled corn fraction, such as a corn flour from which germ and fiber have been removed, is subjected to hydrolysis, typically catalyzed with acid or an enzyme such as an α-amylase enzyme, under conditions suitable to form a mixture of malto-oligosaccharides. A gluten fraction is removed and the enzyme is inactivated, such as with heat. The mixture of malto-oligosaccharides then may be recovered from remaining solids and purified. | 2015-12-10 |
20150353980 | PREPARATION OF 7-DEHYDROCHOLESTEROL AND/OR THE BIOSYNTHETIC INTERMEDIATES AND/OR SECONDARY PRODUCTS THEREOF IN TRANSGENIC ORGANISMS - The present invention relates to a method for preparing 7-dehydrocholesterol and/or the biosynthetic intermediates and/or secondary products thereof by culturing organisms, in particular yeasts. Furthermore, the invention relates to the preparation of the nucleic acid constructs required for preparing the genetically modified organisms and to said genetically modified organisms, in particular yeasts, themselves. | 2015-12-10 |
20150353981 | DISCRIMINATION METHOD FOR MUTATION-INDUCED UNICELLULAR ORGANISM AND MICROFLUIDIC DEVICE USED THEREFOR - The present invention discloses a microfluidic photoreaction system and a method for screening a single cell organism having changed light-response characteristics. According to the present invention, an improved single cell organism can be effectively screened based on phototaxis using a microfluidic system. Specifically, easy monitoring at the cellular level is possible, and a mutant strain having an increased response and/or sensitivity to light can be easily and rapidly screened by various analyses, including statistical analysis of collected results. Thus, the present invention can be effectively used to investigate the correlation between phototaxis and photoconversion efficiency and to screen a single cell organism having increased photosynthetic efficiency. | 2015-12-10 |
20150353982 | MICRO-EVOLUTION OF MICROBES - An enclosed bioremediation system utilizing resistant micro-evolved microbes for treatment of wastewater and recovery of chemicals and metals and which also results in biomass and biochemical production and carbon capture. | 2015-12-10 |
20150353983 | MEANS, METHOD AND COMPUTER PROGRAM PRODUCT FOR DETERMINING THE CONCENTRATION LEVEL OF MICROORGANISMS DURING A FLUID ANALYSIS - A locating means ( | 2015-12-10 |
20150353984 | BIOLOGICAL STERILIZATION INDICATOR SYSTEM AND METHOD - A biological sterilization indicator (BI) system and method. The system can include a BI and a reading apparatus comprising a well. The BI can include a housing, which can include a first portion, and a second portion movable between a first “unactivated” position and a second “activated” position. The BI can further include a frangible container containing a liquid and dimensioned to be positioned in the housing. The reading apparatus can be configured to detect activation of the biological sterilization indicator, for example, by detecting that the second portion is in the second position, and/or by detecting that the liquid from the frangible container is present in a specific chamber of the biological sterilization indicator. The method can include positioning the BI in the well of the reading apparatus and detecting activation, for example, by detecting one or more of the above conditions. | 2015-12-10 |
20150353985 | METHODS FOR ASSAYING ALPHA-L-IDURONIDASE ENZYMATIC ACTIVITY - Methods for assaying α-L-iduronidase enzymatic activity and methods for screening newborns for Mucopolysaccharidosis Type-I. | 2015-12-10 |
20150353986 | METHODS FOR TREATMENT OF CORONARY HEART DISEASE EVENTS BASED ON LIPOPROTEIN-ASSOCIATED PHOSPHOLIPASE A2 ACTIVITY - Methods of treating coronary heart disease (CHD) events by measuring Lp-PLA2 activity are described herein. Described herein are methods that compare Lp-PLA2 activity levels to a binary cut point to guide clinical diagnosis and treatment of CHD. The methods described herein provide robust treatment of patients regardless of race or gender, and may allow the simplification of complex treatment decisions and enhance patient care. | 2015-12-10 |
20150353987 | Rapid Tests for the Detection of Inhibitors of Enzymes and Human Exposure to the Same - A device and method for the rapid on-site detection of inhibitors of enzymes, such as, acetylcholinesterase is described where the device contains 2 reaction zones containing a reporter enzyme substrate. One reaction zone is for the test sample while the other is for an onboard negative control. Sample and control fluids are preincubated with the enzyme in separate reaction containers, then an aliquot of each reaction mixture is added to designated reaction zones on the test device. A purpose-built reader or an illuminating device, such as, containing an incandescent light source, a diode, a UV light source or any other illumination source that is suitable for the reporter or mere visualization is used to determine the level of reporter. | 2015-12-10 |
20150353988 | Borrelia Provocation Procedure Kit - The testing for the Lyme disease pathogen, ( | 2015-12-10 |
20150353989 | SAMPLE PREPARATION FOR NUCLEIC ACID AMPLIFICATION - Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of obtaining nucleic acids material directly from tissues such as whole blood or FFPE samples. | 2015-12-10 |
20150353990 | Test Cartridge with Integrated Transfer Module - A system that includes a cartridge housing and a hollow transfer module, according to an embodiment is described herein. The cartridge housing further includes at least one sample inlet, a plurality of storage chambers, a plurality of reaction chambers, and a fluidic network. The fluidic network is designed to connect the at least one sample inlet, a portion of the plurality of storage chambers and the portion of the plurality of reaction chambers to a first plurality of ports located on an inner surface of the cartridge housing. The hollow transfer module includes a second plurality of ports along an outer surface of the transfer module that lead to a central chamber within the transfer module. The transfer module is designed to move laterally within the cartridge housing. The lateral movement of the transfer module aligns at least a portion of the first plurality of ports with at least a portion of the second plurality of ports. | 2015-12-10 |
20150353991 | MIRNA TARGETS - The present invention provides systems and methods for identifying, isolating, and/or characterizing microRNAs, their targets, and microRNA response elements, and for predicting their biological function. | 2015-12-10 |
20150353992 | RT-qPCR analysis of micro-dissected material from stained FFPET section - The present invention refers to a method for immuno-histochemical staining of a formalin-fixed, paraffin-embedded tissue section comprising the steps of a) providing a solid support, b) mounting the formalin-fixed, paraffin-embedded tissue section onto the solid support, c) removing the paraffin from the formalin-fixed, paraffin-embedded tissue section, d) heating the tissue section mounted on the solid support to retrieve epitopes at 50 to 70° C. for 12 to 24 h, and e) staining the tissue section mounted on the solid support, wherein at least step e) is performed in the presence of 0.5 to 3.0 M sodium chloride. The present invention further refers to a kit for performing the method. | 2015-12-10 |
20150353993 | COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT - The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing. | 2015-12-10 |
20150353994 | BI-DIRECTIONAL SEQUENCING COMPOSITIONS AND METHODS - In some embodiments, methods for obtaining sequence information from a nucleic acid template linked to a support include hybridizing a first primer to a template strand linked to a support, sequencing a portion of the nucleic acid template, thereby forming an extended first primer product that is complementary to a portion of the nucleic acid template, In some embodiments, the method further includes introducing a nick into a portion of the template strand that is hybridized to the extended first primer product, degrading a portion of the template strand from the nick using a degrading agent, where a portion of the extended first primer remains hybridized to an undegraded portion of the template strand, and sequencing at least some of the single-stranded portion of the extended first primer by synthesis. | 2015-12-10 |
20150353995 | ECF-BINDING AGENTS AND USES THEREOF - The invention features a method for isolating a population of pathogenic | 2015-12-10 |
20150353996 | Reusable Long Period Microfiber Grating for detection of DNA Hybridization - A label free, reusable and high sensitivity viral fiber sensor is provided in the present invention. The label free, high sensitivity and reusability are the advantages of this sensor. Long Period Microfiber Grating (LPMFG) is used for the sensing device. It allows optically detecting the change in refractive index at the grating surface with an extra high sensitivity. This provides an optical detection method to monitor DNA Hybridization. The single stranded DNA (ssDNA) probe is immobilized onto the LPMFG's surface for hybridizing with a DNA sample in order to identify the viral strain in the sample. This LPMFG-based viral sensor functions by inducing a refractive index change on the grating surface through the bio-molecule binding between the target viral ssDNA and the immobilized probe ssDNA. Regeneration of a surface-immobilized probe without a significant loss of hybridization activity retains at least 10 successive assays without any significant loss of performance (less than 10% decrease). | 2015-12-10 |
20150353997 | DETECTION OF DNA OR RNA USING SINGLE MOLECULE ARRAYS AND OTHER TECHNIQUES - Described herein are methods and systems for detecting DNA or RNA using single molecules array or other techniques. DNA or RNA from the sample may be fragmented and exposed to a first type of binding ligand and a second type of binding ligand that comprise nucleic acid sequences complimentary at least a portion of a sequence contained in the target DNA or RNA. At least a portion of the fragmented DNA or RNA associates with at least one of the first type of binding ligand and/or the second type of binding ligand, wherein the first type of binding ligand and second type of binding ligand comprises nucleic acid sequences complimentary to a different portions of a sequence contained in the DNA or RNA. A portion of the sample exposed to the binding ligands is analyzed to determine the number of fragmented DNA or RNA sequences. | 2015-12-10 |
20150353998 | COMPOSITIONS AND METHODS FOR REPRESENTATIONAL SELECTION OF NUCLEIC ACIDS FROM COMPLEX MIXTURES USING HYBRIDIZATION - The invention provides a method of selecting a representational sample of nucleic acid sequences from a complex mixture. The method includes: (a) contacting a complex mixture of nucleic acids under conditions sufficient for hybridization with a population of capture probes complementary to one or more nucleic acids comprising a predetermined portion of the sequence collectively present in the complex mixture to form hybridization complexes of the one or more nucleic acids with the population of probes, the population of capture probes being attached to a solid support, and (b) removing unhybridized nucleic acids to select a representational sample of nucleic acids having a complexity of less than 10% but more than 0.001% of the complex mixture, wherein the representational sample comprises a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of the sequences in the predetermined portion of one or more nucleic acids within the complex mixture. A method of selecting a representational sample of genomic sequences from a complete genome also is provided. The invention further provides a nucleic acid population that includes a representational sample having a complexity of less than 10% but more than 0.001% of a complex mixture, the representational sample comprising a nucleic acid copy having a proportion of each sequence in the copy relative to all other sequences in the copy substantially the same as the proportions of sequences in a predetermined portion of a sequence collectively present in one or more nucleic acids within the complex mixture. | 2015-12-10 |
20150353999 | ASSAY AND OTHER REACTIONS INVOLVING DROPLETS - The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention. | 2015-12-10 |
20150354000 | METHOD OF ANALYSIS OF COMPOSITION OF NUCLEIC ACID MIXTURES - When sequencing is used for the analysis of composition of nucleic acid mixtures with a large dynamic range of concentrations of individual components, the reliability of results significantly differs for abundant and rare components. The present invention relates to methods for analysis of concentrations of components of nucleic acid mixtures by sequencing, wherein relative abundances of at least two components for which concentrations should be measured is changed before sequencing in a reproducible way using locus-specific oligonucleotides. | 2015-12-10 |
20150354001 | HYBRID NANOPORES AND USES THEREOF FOR DETECTION OF ANALYTES - The invention relates to a hybrid structure including perforated solid substrate having at least one nanopore perforating therethrough, and devices and uses thereof. | 2015-12-10 |
20150354002 | Identification of Traits Associated with DNA Samples Using Epigenetic-Based Patterns Detected Via Massively Parallel Sequencing - Illustrative embodiments of systems and methods for the identification of traits associated with DNA samples using epigenetic-based patterns detected via massively parallel sequencing (MPS) are disclosed. Illustrative embodiments may involve digesting a DNA sample with a methylation-dependent endonuclease, amplifying loci of the digested DNA sample (including a positive control locus that does not contain a restriction site for the methylation-dependent endonuclease) using a multiplex PCR to produce amplicons, sequencing the amplicons using an MPS instrument to generate sequence reads, determining a sequence count for each of the loci by comparing each of the sequence reads to reference sequences, normalizing the sequence count for each of the loci to the sequence count of the positive control locus, and identifying a trait associated with the DNA sample by applying a classification algorithm to the normalized sequence counts. | 2015-12-10 |
20150354003 | METHODS FOR ANALYZING NUCLEIC ACIDS - The invention generally relates to methods for analyzing nucleic acids. In certain aspects, methods of the invention involve obtaining a sample including a nucleic acid template. A plurality of molecular inversion probes are tiled across a portion of the template. The probes are designed such that immediately adjacent probes hybridize to opposite strands of the nucleic acid template and probes on the same strand hybridize to the template in an overlapping manner. A region between targeting arms of a plurality of the molecular inversion probes is filled-in with nucleotides, and the filled-in region of a plurality of the probes is analyzed to obtain sequence information about the nucleic acid template. | 2015-12-10 |
20150354004 | Method for Nondestructive Detection of MiRNA Expression in Cell and Determination of Cell Type and State - The present invention provides a method for non-invasively detecting the expression levels of miRNAs in cell media and determining the types and status of the cells, and specifically provides a method for determining the type and status of the cells according to the expression level of miRNAs in a cell medium. The method comprises: culturing different types of cells, collecting the medium of the cells, extracting RNAs from the medium, performing reverse transcription on the RNAs, detecting miRNAs in the cell medium by the fluorescent quantitative PCR method, and determining the types and status of the detected cells according to the relationship between the expression levels of miRNAs in different types and status of cells and the expression level of the detected miRNAs. The method of the present invention proves for the first time that the expression levels of miRNAs in the medium can be detected to determine the types and status of cells, comprising the pluripotency level of stem cells and the status of cells obtained through transdifferentiation, so as to avoid causing damages to cells, and is especially suitable for the experimental and clinical applications in which the number of cells is limited. | 2015-12-10 |
20150354005 | METHOD FOR PREDICTING THE ONSET OF EXTRAPYRAMIDAL SYMPTOMS (EPS) INDUCED BY AN ANTIPSYCHOTIC-BASED TREATMENT - The invention relates to methods for predicting the onset of extrapyramidal symptoms (EPS) induced by an antipsychotic-based treatment as well as methods for providing personalized medicine to patients based on the sequence of several SNPs associated with the onset of EPS. The invention relates as well to kits for carrying out the diagnostic and predictive medicine methods. | 2015-12-10 |
20150354006 | MARKERS FOR ACUTE LYMPHOBLASTIC LEUKEMIA - The invention provides methods of detecting a NSD2 mutation in a cancer cell, methods cancer diagnosis and methods of screening for NSD2 inhibitors. | 2015-12-10 |
20150354007 | SIALYLTRANSFERASE ST3GAL6 AS A MARKER FOR MULTIPLE MYELOMA - The present invention relates to the sialyltransferase ST3GAL6 for use as a biomarker for multiple myeloma, and especially as a marker for myelomas with inferior survival rates. The inventors have shown that glycosylation gene expression is dysregulated in Multiple Myeloma and that overexpression of the sialyltransferase ST3GAL6 is associated with inferior survival rates in patients. | 2015-12-10 |
20150354008 | DIAGNOSTIC FOR LUNG DISORDERS USING CLASS PREDICTION - The present invention provides methods for diagnosis and prognosis of lung cancer using expression analysis of one or more groups of genes, and a combination of expression analysis with bronchoscopy. The methods of the invention provide far superior detection accuracy for lung cancer when compared to any other currently available method for lung cancer diagnostic or prognosis. The invention also provides methods of diagnosis and prognosis of other lung diseases, particularly in individuals who are exposed to air pollutants, such as cigarette or cigar smoke, smog, asbestos and the like air contaminants or pollutants. | 2015-12-10 |
20150354009 | COLORECTAL CANCER CLASSIFICATION WITH DIFFERENTIAL PROGNOSIS AND PERSONALIZED THERAPEUTIC RESPONSES - The present invention relates to gene sets, the expression levels of which are useful for classifying colorectal tumors and predicting disease-free prognosis and response of patients to specific therapies that are either novel or currently available in the clinics for colorectal cancer patients. | 2015-12-10 |
20150354010 | MARKERS FOR BREAST CANCER - Correlations between polymorphisms and breast cancer are provided. Methods of diagnosing, prognosing, and treating breast cancer are provided. Systems and kits for diagnosis, prognosis and treatment of breast cancer are provided. Methods of identifying breast cancer modulators are also described. | 2015-12-10 |
20150354011 | METHODS FOR DIAGNOSING CANCER BASED ON SMALL NUCLEOLAR RNA HBII-52 - Provided are compositions and methods for detecting in a sample the presence or absence, and/or the amount, of a small nucleolar RNA (snoRNA) HBII-52, also known as SNORD115. The compositions and methods are useful in diagnosis, prognosis, therapy recommendations, therapy, and monitoring of therapy for individuals who have a disorder that is positively correlated with elevated HBII-52, such as cancer, and particularly for prostate cancer. Kits containing primers for detecting and/or amplifying HBII-52 from a biological sample are provided. The disclosure includes a method for monitoring an individual undergoing therapy for a disorder associated with HBII-52 expression, a method for identifying an individual as a candidate for therapy with an antagonist of 5-HT | 2015-12-10 |
20150354012 | DIAGNOSING IDH1 RELATED SUBGROUPS AND TREATMENT OF CANCER - The invention relates to classification, diagnosis and treatment of cancers. In one embodiment, the present invention provides methods and kits that classify cancers into various subtypes based on expression patterns of AKT pathway components. In another embodiment, the present invention provides methods and kits that diagnose cancer subtypes by evaluating expression patterns of AKT pathway components. In still another embodiment, the present invention provides methods and kits that treat a cancer subtype by administering an alkylating agent or a PI3K/AKT/mTOR inhibitor to a patient. Cancers suitable with various embodiments of the invention include but are not limited to brain tumors, gliomas and GBM. | 2015-12-10 |
20150354013 | DETECTING NEOPLASM - This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided. | 2015-12-10 |
20150354014 | Compositions, methods and kits for diagnosis of carriers of mutations in the BRCA1 and BRCA2 genes and early diagnosis of cancerous disorders associated with mutations in BRCA1 and BRCA 2 genes - The present invention relates to diagnostic compositions methods and kits for the detection of carriers of mutations in the BRCA1 and BRCA2 genes. The detection is based on the use of detecting nucleic acids or amino acid based molecules, specific for determination of the expression of at least six marker genes of the invention, in a test sample. The invention thereby provides methods compositions and kits for the diagnosis of cancerous disorders associated with mutations in the BRCA1 and BRCA2 genes, specifically, of ovarian and breast cancer. | 2015-12-10 |
20150354015 | METHOD FOR DIRECT AMPLIFICATION FROM CRUDE NUCLEIC ACID SAMPLES - The present teachings relate to improved methods, kits, and reaction mixtures for amplifying nucleic acids. In some embodiments a novel direct buffer formulation is provided which allows for the direct amplification of the nucleic acids in a crude sample with minimal sample purification. | 2015-12-10 |
20150354016 | COMPOSITIONS, METHODS AND KITS TO DETECT HERPES SIMPLEX VIRUS NUCLEIC ACIDS - The disclosed invention is related to methods, compositions, kits and isolated nucleic acid sequences for targeting Herpes Simplex Virus (HSV) nucleic acid (e.g., HSV-1 and/or HSV-2 nucleic acid). Compositions include amplification oligomers, detection probe oligomers and/or target capture oligomers. Kits and methods comprise at least one of these oligomers. | 2015-12-10 |
20150354017 | METHODS FOR REMOVING RESIDUAL SOLIDS FROM ENZYMATIC HYDROLYSATE TO MAKE PURIFIED FERMENTABLE SUGAR SYRUP - The invention provides a method for purifying a biomass hydrolysate comprising sugars and suspended particles, comprising centrifuging the biomass hydrolysate, thermally treating the centrifuged hydrolysate to chemically or physically agglomerate the suspended particles, and filtering the thermally treated hydrolysate to remove agglomerated suspended particles, thereby generating a purified hydrolysate (sugar syrup). The sequence of steps may be varied. Biomass hydrolysates may be provided from a wide variety of processes. Surprisingly, a 20-fold improvement in sugar purity (total suspended solids content) is demonstrated experimentally, compared to prior methods. | 2015-12-10 |