49th week of 2021 patent applcation highlights part 29 |
Patent application number | Title | Published |
20210380965 | MICRONEEDLE-BASED RAPID ANALYTE EXTRACTION FROM PLANT AND ANIMAL TISSUES AND RELATED METHODS AND SYSTEMS - Methods of extracting analytes of interest from biological samples including soft plant tissues (e.g., plant leaves) or animal tissues are described. The methods include contacting the biological samples with a microneedle that absorbs the analyte of interest. Related systems and methods of detecting pathogens or pests or of genotyping the plant or animal from which the biological sample is derived are described. Also described are methods of delivering a substance of interest (e.g., a pesticide) to a plant by contacting a soft plant tissue with a microneedle coated with the substance of interest. | 2021-12-09 |
20210380966 | METHOD FOR ISOLATING POLY(A) NUCLEIC ACIDS - The present invention pertains inter alia to a method for isolating poly(A) nucleic acids having a single stranded poly(A) stretch from a nucleic acid containing sample comprising: (a) providing a hybridization composition comprising: i) a nucleic acid containing sample; ii) a hybridization solution comprising: aa. a sodium salt; bb. a quaternary ammonium salt; wherein the components of the hybridization solution can be added as single solution to the sample or may be added separately in any order to the sample; iii) a capture probe capable of hybridizing to the poly(A) stretch of the poly(A) nucleic acids; and incubating said hybridization composition under conditions to form nucleic acid-hybrids between the poly(A) nucleic acids and the capture probe; (b) separating the formed hybrids from the remaining sample. The method is in particular suitable for efficiently isolating poly(A) RNA from various samples while avoiding carry-over of unwanted non-poly(A) nucleic acids such as rRNA. Also provided are advantageous further methods, hybridization solutions and kits. | 2021-12-09 |
20210380967 | Methods of Identifying Adenosine-to-Inosine Edited RNA - This disclosure relates to improved methods of identifying A-to-I RNA edits in a sample. In certain embodiments, this disclosure relates to methods of purifying RNA containing an inosine base comprising the steps of: exposing an RNA sample to endonuclease V or fusion thereof and calcium ions in the absence of magnesium ions providing an RNA and endonuclease V binding complex. In certain embodiments, the methods further comprise purifying the RNA and endonuclease V binding complex from unbound RNA in the sample; separating the RNA from endonuclease V providing separated RNA; sequencing the separated RNA; and identifying positions in the RNA sequences wherein A-to-I edits occur. In certain embodiments, the RNA is derived from a cell. | 2021-12-09 |
20210380968 | COMPOSITIONS AND METHODS FOR MULTIPLEX NUCLEIC ACIDS SYNTHESIS - Aspects of the invention relate to methods, compositions for designing and producing a target nucleic acid. In particular, aspects of the invention relate to the multiplex synthesis of target polynucleotides. | 2021-12-09 |
20210380969 | REDIRECTION OF TROPISM OF AAV CAPSIDS - The disclosure relates to compositions, methods, and processes for the preparation, use, and/or formulation of adeno-associated virus capsid proteins, wherein the capsid proteins comprise targeting peptide inserts for enhanced tropism to a target tissue. | 2021-12-09 |
20210380970 | GENERATING RECOMBINANT AFFINITY REAGENTS WITH ARRAYED TARGETS - Methods for screening of affinity reagents for many target proteins of interest simultaneously. Arrayed targets (e.g., peptide, protein, RNA, cell, etc.) are used in affinity selection experiments to reduce the amount of target needed and to improve the throughput of discovering recombinant affinity reagents to a large collection of targets. | 2021-12-09 |
20210380971 | SIMULTANEOUS, SEQUENCING-BASED ANALYSIS OF PROTEINS, NUCLEOSOMES, AND CELL-FREE NUCLEIC ACIDS FROM A SINGLE BIOLOGICAL SAMPLE - The invention provides a method for the analysis of a biological sample to determine multiple types of information therefrom in a streamlined, combined workflow, where all information is obtained in a sequencing-based analysis. The information includes the presence and concentration of specific plasma proteins in a blood sample; the number, location, and types of histone modifications associated with cell-free DNA obtained from the same sample; the sequence of cfRNA and cfDNA in the cell-free DNA sample; and epigenetic information pertaining to the cell-free DNA, such as hydroxymethylation and methylation profiles, i.e., the distribution of 5-hydroxymethylcytosine (5hmC) and 5-methylcytosine (5mC) residues, respectively. The invention additionally pertains to a classical sequencing-based method for analyzing a biological sample to determine one or more non-classical sequence features of the sample. Compositions, kits, and related methods are also provided, including an embodiment in which truncated sequencing adapters are used in conjunction with barcoded PCR primers. | 2021-12-09 |
20210380972 | METHODS FOR INCREASING YIELD OF SEQUENCING LIBRARIES - The present disclosure is concerned with compositions, methods, and kits for preparing a sequencing library. In one embodiment, methods include producing a library of target nucleic acids having the same adapter at each end and then switching the identity of one adapter to result in target nucleic acids flanked by distinct adapters. | 2021-12-09 |
20210380973 | TCR LIBRARIES - The present invention relates to a library of particles, the library displaying a plurality of different T cell receptors (TCRs), wherein the plurality of TCRs consists essentially of TCRs comprising an alpha chain comprising an alpha chain variable domain and a beta chain comprising a beta chain variable domain and the library comprises more than one TRAV gene product and/or more than one TRBV gene product, wherein the beta chain variable domain does not comprise one or more of a TRBV5-1, 5-3, 5-4, 5-5, 5-6, 5-7 or 5-8 gene product and wherein the plurality of TCRs do not consist essentially of TCRs comprising a TRAV12-2 gene product from a natural repertoire and a TRBV6 gene product from a natural repertoire and TCRs comprising a TRAV21 gene product from a natural repertoire and a TRBV6 gene product from a natural repertoire. | 2021-12-09 |
20210380974 | COMBINATORIAL SETS OF NUCLEIC ACID BARCODES FOR ANALYSIS OF NUCLEIC ACIDS ASSOCIATED WITH SINGLE CELLS - Provided herein are methods of identifying the origin of a nucleic acid sample. The methods include forming a reaction mixture comprising a nucleic acid sample comprising nucleic acid molecules from a single cell and a set of barcodes, incorporating the set of barcodes into the nucleic acid molecules of the sample, and identifying the set of barcodes incorporated into the nucleic acid molecules of the single cell thereby identifying the origin of the nucleic acid sample. | 2021-12-09 |
20210380975 | RNA-GUIDED TRANSCRIPTIONAL REGULATION AND METHODS OF USING THE SAME FOR THE TREATMENT OF BACK PAIN - Disclosed herein are compositions and methods for treatment and prevention of low back pain. The compositions include vectors comprising nucleotide sequences encoding one or more CRISPR-Cas system guide RNAs and a RNA-directed nuclease. The methods include modulating expression of one or more genes in a cell using said compositions, introducing a CRISPR-Cas system into a cell comprising one or more vectors comprising said compositions, including site-specific DNA cleavage in a cell, and treating a subject having lower back pain, and lower back pain cause by degenerative disc disease using the compositions disclosed herein. | 2021-12-09 |
20210380976 | CHIRALLY ENRICHED OLIGOMERIC COMPOUNDS - The present disclosure provides oligomeric compounds comprising a modified oligonucleotide having one or more chirally enriched phosphorothioate intemucleoside linkages. In certain embodiments, the modified oligonucleotide decreases expression of target mRNA. | 2021-12-09 |
20210380977 | MYOCARDIAL ENHANCER RNA AND METHODS OF USE - Disclosed are methods of detecting myocardial enhancer RNA levels in mammalian cardiomyocytes. In a further embodiment, a method of disrupting assembly of an enhancer DNA-Myh6 promoter-Myh7 promoter complex, is provided wherein the method comprises providing enhancer RNA to a cardiomyocyte. | 2021-12-09 |
20210380978 | The long non-coding RNA INCA1 and Homo sapiens heterogeneous nuclear ribonucleoprotein H1 (HNRNPH1) as therapeutic targets for immunotherapy - Compositions comprising inhibitory nucleic acids targeting the long non-coding RNA INCA1, and methods of use thereof, e.g., in combination with immunotherapy, to treat cancer. | 2021-12-09 |
20210380979 | MULTIMERIC OLIGONUCLEOTIDES HAVING DECREASED KIDNEY CLEARANCE - The present invention relates to methods of administering to a subject multimeric oligonucleotides having monomeric subunits joined by linkers. The multimeric oligonucleotides have a molecular weight of at least about 45 kD and other characteristics, such that their clearance due to glomerular filtration is reduced. The present invention also relates to such multimeric oligonucleotides and methods of synthesizing such multimeric oligonucleotides. | 2021-12-09 |
20210380980 | Methods and Compositions for the ADAR-Mediated Editing of SERPINA1 - The present invention relates to methods and compositions for editing a SERPINA1 polynucleotide, e.g., a SERPINA1 polynucleotide comprising a SNP associated with alpha 1 antitrypsin deficiency. The invention also relates to methods and compositions for treating or preventing alpha 1 antitrypsin deficiency in a subject. | 2021-12-09 |
20210380981 | ANTI-ANGIOGENIC MIRNA THERAPEUTICS FOR INHIBITING CORNEAL NEOVASCULARIZATION - The disclosure relates, in some aspects, to compositions and methods for treating corneal disease (e.g., corneal neovascularization. In some embodiments, the disclosure relates to rAAV-mediated delivery of an cornea-associated transgene to a subject. In some embodiments, the rAAV transduces the corneal tissue of a subject. | 2021-12-09 |
20210380982 | ANTISENSE OLIGONUCLEOTIDE-BASED PROGRANULIN AUGMENTATION THERAPY IN NEURODEGENERATIVE DISEASES - Described herein are methods and compositions related to the modulation of progranulin expression or activity in the brain for the treatment of neurodegenerative diseases. | 2021-12-09 |
20210380983 | ENGINEERING PLANT GENOMES USING CRISPR/Cas SYSTEMS - Materials and methods for gene targeting using Clustered Regularly Interspersed Short Palindromic Repeats/CRISPR-associated (CRISPR/Cas) systems are provided herein. | 2021-12-09 |
20210380984 | COMPOSITIONS AND METHODS FOR INHIBITING ALDH2 EXPRESSION - This disclosure relates to oligonucleotides, compositions and methods useful for reducing ALDH2 expression, particularly in hepatocytes. Disclosed oligonucleotides for the reduction of ALDH2 expression may be double-stranded or single-stranded, and may be modified for improved characteristics such as stronger resistance to nucleases and lower immunogenicity. Disclosed oligonucleotides for the reduction of ALDH2 expression may also include targeting ligands to target a particular cell or organ, such as the hepatocytes of the liver, and may be used to treat alcoholism and related conditions. | 2021-12-09 |
20210380985 | 17 -Hydroxysteroid Dehydrogenase Type 13 (HSD17B13) iRNA Compositions And Methods Of Use Thereof - The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the HSD17B 13 gene, as well as methods of inhibiting expression of HSD17B13, and methods of treating subjects that would benefit from reduction in expression of HSD17B13, such as subjects having a HSD17B13-associated disease, disorder, or condition, using such dsRNA compositions. | 2021-12-09 |
20210380986 | RNA DRUGS AND METHOD OF TREATING AGE-RELATED DISEASE - Mouse models show that decreasing the expression of pregnancy-associated plasma protein-A (PAPP-A) can reduce age-related muscle atrophy, cancer, atherosclerosis, and increase immune function and longevity. The invention describes RNA agents designed to reduce the expression of pregnancy-associated plasma protein-A. The invention further describes delivering these RNA agents to subjects in vivo as a method of preventing age-related disease and thymic atrophy. | 2021-12-09 |
20210380987 | CRISPR/CAS-RELATED METHODS AND COMPOSITIONS FOR TREATING CYSTIC FIBROSIS - CRISPR/CAS-related compositions and methods for treatment of Cystic Fibrosis (CF). | 2021-12-09 |
20210380988 | Reducing Prominin2-Mediated Resistance to Ferroptotic Cell Death - Compositions and methods for inducing tumor cell death by inhibiting prominin2 (PROM2) or heat shock factor protein 1 (HSF1), optionally in combination with an agent that promotes ferroptosis, preferably an inhibitor of glutathione peroxidase 4 (GPX4), e.g., to trigger ferroptosis in the tumor cells. | 2021-12-09 |
20210380989 | MODULATION OF NADPH GENERATION BY RECOMBINANT YEAST HOST CELL DURING FERMENTATION - The present disclosure concerns recombinant yeast host cells having a first genetic modification for downregulating a first metabolic pathway that converts NADP | 2021-12-09 |
20210380990 | SYNTHETIC IMMUNOMODULATION WITH A CRISPR SUPER-REPRESSOR IN VIVO - Provided herein are CRISPR-based synthetic repression systems as well as methods and compositions using the synthetic repression systems to treat septicemia, an adverse immune response in a subject and Waldanstrom macroglobulinemia. | 2021-12-09 |
20210380991 | COMPOSITIONS AND METHODS FOR HIGH FIDELITY ASSEMBLY OF NUCLEIC ACIDS - Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid. In certain embodiments, the plurality of blunt-end double-stranded nucleic acid fragments can be provided by: releasing a plurality of oligonucleotides synthesized on a solid support; and synthesizing complementary strands of the plurality of oligonucleotides using a polymerase based reaction. | 2021-12-09 |
20210380992 | METHOD OF MAKING RECOMBINANT SILK AND SILK-AMYLOID HYBRID PROTEINS USING BACTERIA - Disclosed are methods of making recombinant secretion of silk and collagen proteins, and amyloid fusions thereof, using bacteria. | 2021-12-09 |
20210380993 | OPTIMIZED VECTOR FOR DELIVERY IN MICROBIAL POPULATIONS - The present invention relates to a vector, preferably included in a delivery vehicle, comprising no more than 100, preferably no more than 10, restriction sites recognized by the restriction enzymes encoded by each bacterium of a group of bacteria of interest. The invention also relates to the use of said vector, preferably included in a delivery vehicle, as a drug, especially in the treatment of a disease in a patient in need thereof. | 2021-12-09 |
20210380994 | LACTOCOCCUS LACTIS EXPRESSION SYSTEM FOR DELIVERING PROTEINS EFFICACIOUS FOR THE TREATMENT OF EPITHELIAL BARRIER FUNCTION DISORDERS - The disclosure relates to live biotherapeutic products, probiotics, and therapeutic composition comprising said probiotics having therapeutic proteins, and methods of using them to treat various human diseases. In particular aspects, the disclosure provides such compositions comprising strains of the | 2021-12-09 |
20210380995 | MICROBIAL CELL WITH IMPROVED IN VIVO CONVERSION OF THEBAINE/ORIPAVINE - A recombinant microbial host cell having improved in vivo conversion of reticuline and derivatives thereof (such as thebaine and/or oripavine) to relevant downstream opioids (such as neopinone, oripavine, northebaine, nororipavine or morphinone) and related compounds (such as heroin, morphine, codeine, thebaine, oripavine, oxycodone, hydrocodone, hydromorphone, oxymorphone, buprenorphine, naltrexone, naloxone or nalbuphine), wherein the microbial (such as fungal) host cell is heterologously expressing at least one functional transporter protein capable of transporting reticuline or a derivative thereof (such as thebaine and/or oripavine) and a heterologously expressed enzyme capable of acting upon reticuline or a derivative thereof. The invention also relates to uses of the microbial host cells and methods of making an opioid compound and/or opioid precursor compound and/or opioid derivative of interest. | 2021-12-09 |
20210380996 | MICROORGANISMS ENGINEERED TO USE UNCONVENTIONAL SOURCES OF NITROGEN - Disclosed are genetically engineered organisms, such as yeast and bacteria, that have the ability to metabolize atypical nitrogen sources, such as melamine and cyanamide. Fermentation methods using the genetically engineered organisms are also described. The methods of the invention are robust processes for the industrial bioproduction of a variety of compounds, including commodities, fine chemicals, and pharmaceuticals. | 2021-12-09 |
20210380997 | GLUCANASE PRODUCTION AND METHODS OF USING THE SAME - Methods and compositions are described for producing a glucanase in transgenic plants and then incorporating parts of the transgenic plants in animal feed. The feed glucanase enzyme displays activity across a broad pH range, and tolerance to temperatures that are often encountered during the process of preparing animal feeds. Producing the enzyme in the transgenic plant enhances the thermal stability of the enzyme. | 2021-12-09 |
20210380998 | Synthetic Cannabinoid Compounds for Treatment of Substance Addiction and Other Disorders - A method of increasing the cannabinoid levels in a genetically modified | 2021-12-09 |
20210380999 | PLANTS HAVING INCREASED TOLERANCE TO HERBICIDES - The present invention refers to a method for controlling undesired vegetation at a plant cultivation site, the method comprising the steps of providing, at said site, a plant that comprises at least one nucleic acid comprising a nucleotide sequence encoding a wild-type or a mutated protoporphyrinogen oxidase (PPO) which is resistant or tolerant to a PPO-inhibiting herbicide by applying to said site an effective amount of said herbicide. The invention further refers to plants comprising wild-type or mutated PPO enzymes, and methods of obtaining such plants. | 2021-12-09 |
20210381000 | MAIZE EVENT DP-915635-4 AND METHODS FOR DETECTION THEREOF - Embodiments disclosed herein relate to the field of plant molecular biology, specifically to DNA constructs for conferring insect resistance to a plant. Embodiments disclosed herein relate to insect resistant corn plant containing event DP-915635-4, and to assays for detecting the presence of event DP-915635-4 in samples and compositions thereof. | 2021-12-09 |
20210381001 | A METHOD TO TREAT DISEASE USING A NUCLEIC ACID VECTOR ENCODING A HIGHLY COMPACT MULTI-INPUT LOGIC GATE - Disclosed herein are contiguous DNA sequences encoding highly compact multi-input genetic logic gates for precise in vivo cell targeting, and methods of treating disease using a combination of in vivo delivery and such contiguous DNA sequences. | 2021-12-09 |
20210381002 | GENE THERAPY FOR OXIDATIVE STRESS - Compositions and methods for anti-oxidant therapy are provided. | 2021-12-09 |
20210381003 | GENE THERAPY VECTORS FOR TREATMENT OF DANON DISEASE - The disclosure relates to optimized polynucleotide sequences for LAMP-2B, expression cassettes, vectors, and methods of use thereof in treating disease, e.g. Danon disease. | 2021-12-09 |
20210381004 | RAAV Vectors for the Treatment of GM1 and GM2 Gangliosidosis - Aspects of the disclosure relate to compositions and methods for the treatment of lysosomal storage disorders, such as GM1 gangliosidosis, Tay Sachs disease, and Sandhoff disease. In some embodiments, the compositions comprise viral vectors encoding beta-galactosidase. In some embodiments, the compositions comprise viral vectors encoding beta-hexosaminidase subunits (e.g. HEXA, HEXB, or combinations thereof). | 2021-12-09 |
20210381005 | TEMPERATURE-BASED TRANSIENT DELIVERY OF NUCLEIC ACIDS AND PROTEINS TO CELLS AND TISSUES - The present disclosure relates to methods for transiently activating temperature-sensitive agents in one or more cells, for example by contacting one or more cells with a temperature-sensitive agent and transiently incubating the cells at a permissive temperature for inducing an activity of the temperature-sensitive agent in the cells. Additionally, the present disclosure relates to methods of contacting one or more cells in a subject with a temperature-sensitive agent and then lowering the subject's core body temperature to a permissive temperature for inducing an activity of the temperature-sensitive agent in the cells. The disclosure also relates to methods of contacting one or more cells in a subject with a temperature-sensitive agent, maintaining the subject's surface body temperature at a permissive temperature for inducing an activity of the temperature-sensitive agent in the cells. Further disclosed are methods of treating a subject with a temperature-sensitive therapeutic agent. | 2021-12-09 |
20210381006 | METHOD FOR ACTIVATION/PROLIFERATION OF T CELLS - The present invention provides a method for activating/proliferating T cells, including a step of contacting a cell population containing T cells with a nucleic acid delivery carrier having at least one kind of T cell activating ligand added to its surface, and a method for delivering a nucleic acid into T cells, the methods including a step of contacting a cell population containing T cells simultaneously with (a) a nucleic acid delivery carrier having at least one kind of T cell activating ligand added to its surface and containing a nucleic acid inside, or (b) at least one kind of T cell activating ligand, and a nucleic acid delivery carrier containing a nucleic acid inside and free of a T cell activating ligand added to its surface, a method for producing a medicament containing T cells and the like. | 2021-12-09 |
20210381007 | METHODS AND COMPOSITIONS FOR CRISPR EDITING OF CELLS AND CORRELATING THE EDITS TO A RESULTING CELLULAR NUCLEIC ACID PROFILE - The present disclosure provides compositions, methods and modules to edit live cells and to subsequently correlate the resulting cellular nucleic acids of the edited cells to the edits. | 2021-12-09 |
20210381008 | METHOD FOR PRODUCING FOREIGN ANTIGEN RECEPTOR GENE-INTRODUCED CELL - Provided is a method for producing a cell incorporating an antigen specific receptor gene, which comprises the step of introducing an exogenous TCR or CAR gene into a material cell so that the introduced gene is expressed under the T cell receptor expression control system of the material cell. | 2021-12-09 |
20210381009 | Enzyme-Expressing Yeast For Ethanol Production - Described herein are recombinant fermenting organisms having a heterologous polynucleotide encoding a phospholipase. Also described are processes for producing a fermentation product, such as ethanol, from starch or cellulosic-containing material with the recombinant fermenting organisms. | 2021-12-09 |
20210381010 | CARBON CAPTURE IN FERMENTATION - The disclosure relates to methods of capturing carbon by microbial fermentation of a gaseous substrate comprising CO into one or more first products which, in turn, may be incorporated into an article of manufacture or one or more second products. Further, the disclosure relates to improving carbon capture and/or efficiency. | 2021-12-09 |
20210381011 | YEAST CELLS HAVING REDUCTIVE TCA PATHWAY FROM PYRUVATE TO SUCCINATE AND OVEREXPRESSING AN EXOGENOUS NAD(P+) TRANSHYDROGENASE ENZYME - Yeast cells having a reductive TCA pathway from pyruvate or phosphoenolpyruvate to succinate, and which include at least one exogenous gene overexpressing an enzyme in that pathway, further contain an exogenous transhydrogenase gene. | 2021-12-09 |
20210381012 | BIOPOLYMER FILM AND METHOD OF PREPARING THE SAME - A method of producing biopolymer films is disclosed. The method includes pre-treating a carbon source, preparing a basal media, preparing an inoculum and fermenting the carbon source using the inoculum in the basal media so as to selectively modify the metabolic pathway of the microorganism to produce a biopolymer. Further, the method includes recovering the biopolymer resulting from the step of fermentation and blending the biopolymer with at least one blending agent to produce one or more biopolymer films. | 2021-12-09 |
20210381013 | ENZYMATIC CYCLIZATION OF HOMOFARNESYLIC ACID - The present invention relates to processes for the preparation of sclareolide and related compounds by the biocatalytic cyclization of polyunsaturated carboxylic acid compounds, in particular of homofarnesylic acid and related compounds; and to a process for the preparation of ambroxide via the biocatalytic cyclization of homofarnesylic acid to sclareolide. | 2021-12-09 |
20210381014 | ENZYMATIC PRODUCTION OF HEXOSES - Disclosed herein are methods of producing hexoses from saccharides by improved enzymatic processes. The improved processes utilize enzymes with higher activities than those previously reported to convert starch or a starch derivative, cellulose or a cellulose derivative, or sucrose to a glucose 6-phosphate (G6P) intermediate. | 2021-12-09 |
20210381015 | IMMUNOTHERAPEUTIC POTENTIAL OF MODIFIED LIPOOLIGOSACCHARIDES/LIPID A - Embodiments of the disclosure provide for unique lipooligosaccharide/lipid A-based mimetics for use as adjuvants. Methods of generating lipooligosaccharide/lipid A-based mimetics are provided that utilize recombinantly engineered bacteria to produce the mimetics, including, for example, addition of one or more particular enzymes such as acyltransferases, deacylases, phosphatases, or glycosyltransferases. | 2021-12-09 |
20210381016 | METHODS FOR STARCH HYDROLYSIS - The presently disclosed subject matter provides a process for starch liquefaction using at least two classes of α-amylase enzymes, wherein the starch hydrolysis pattern from at least two of these classes is different. At least one class of enzyme is provided to the liquefaction process in the form of transgenic plant material expressing at least one class of α-amylase enzyme or is provided in the form of a purified or partially-purified α-amylase enzyme preparation. The second or subsequent class(es) of α-amylase enzymes may be provided in the form of additional transgenic plant material expressing the second or subsequent class(es), or may be provided in the form of a second or subsequent purified or partially-purified α-amylase enzyme preparation. | 2021-12-09 |
20210381017 | PROCESS FOR TREATING LIGNOCELLULOSIC BIOMASS - The present invention relates to a process for treating lignocellulosic biomass, successively comprising
| 2021-12-09 |
20210381018 | METHODS AND SYSTEMS FOR MONOMER CHAIN FORMATION - Aspects relate to systems and methods for high-contrast cleavage detection. An exemplary method includes a plurality of probes, wherein at least a probe of the plurality of probes comprises a high-contrast agent, a waveguide configured to propagate an electromagnetic (EM) wave, wherein the waveguide includes a surface upon which the plurality of probes are immobilized, wherein the surface is configured to provide communication between the EM wave and the high-contrast agent, a primary cleaving agent proximal the plurality of probes, wherein the primary cleaving agent and the plurality of probes are configured to selectively cleave the at least a probe as a result of the primary cleaving agent being in presence of an analyte, and a sensor in communication with the waveguide and configured to detect cleaving of the at least a probe by way of the EM wave. | 2021-12-09 |
20210381019 | BIOSYNTHETIC PRODUCTION OF VARIANT STEVIOL GLYCOSIDES - The present invention relates to novel steviol glycosides R6-1, R6-2A, R6-2B, R6-4A, R6-4B and R7-2 and the production of these novel steviol glycosides, such as through enzymatic bioconversion. The use of these novel steviol glycosides as sweeteners and in orally consumable products are also provided. | 2021-12-09 |
20210381020 | METHOD FOR THE PRODUCTION OF A GLYCOSYLATED IMMUNOGLOBULIN - Herein is reported a method for the production of an immunoglobulin comprising the following steps: a) providing a eukaryotic cell comprising a nucleic acid encoding the immunoglobulin, b) cultivating the eukaryotic cell in a cultivation medium wherein the amount of glucose available in the cultivation medium per time unit is kept constant and limited to less than 80% of the amount that could maximally be utilized by the cells in the cultivation medium per time unit, and c) recovering the immunoglobulin from the culture. | 2021-12-09 |
20210381021 | METHOD OF EXTRACTING A PIGMENT FROM MICROALGAE - The present invention relates to a method of extracting a pigment from microalgae. | 2021-12-09 |
20210381022 | INTEGRATED DRUG DISCOVERY PLATFORM FOR INBORN ERROR OF METABOLISM DISORDERS - The invention relates to in vivo systems and high throughput screening platform for drugs applicable in inborn error of metabolism (IEM) disorders. More specifically, the invention provides yeast screening system of candidate therapeutic compounds applicable in IEM disorders associated with accumulation of at least one metabolite. The systems of the invention comprise yeast cell/s that carry at least one manipulation in at least one yeast metabolic pathway, that leads to accumulation of said metabolite. | 2021-12-09 |
20210381023 | PERSONAL PROTECTION AND MONITORING - A personal protection device includes one or two elongate members for engaging into the nostrils of the nose of a user. The elongate members each includes gridded ports for allowing air to pass through the elongate member when breathing in and for allowing air to exit via the elongate member when breathing out. Sensors are placed in the air flow pathway to detect pathogen presence, and the primary air filter is positioned in the elongate member. In yet other embodiments, a disposable version is disclosed. The eyewear can also be used as part of an extended reality system, and when game playing, particularized scents can be rendered to the nose to enhance the gaming experience. In another aspect, systems and methods protect against pathogen by sampling an environment of a travel path with a plurality of pathogen detectors along the travel path to detect a presence of one or more pathogens, wherein at least one detector includes a nano-sensor with receptacles to bind to the pathogens and wherein the nano-sensor changes resistivity, inductance or capacitance upon pathogen binding; directing air towards said pathogen detectors; contact tracing a user mobile device having a mobile identification (ID) carried by each user, wherein the mobile device comprises a memory storing mobile IDs of all devices within a predetermined radius of the user mobile device; and performing deep learning with a neural network receiving data from the pathogen detectors and to the user mobile device to detect a presence of one or more pathogens. | 2021-12-09 |
20210381024 | Personalized System for Restoring the Gastrointestinal Tract Microbiome - The present invention provides methods for enhancing the health of a subject using microorganisms indigenous to the subject's own gut microbiome. In one embodiment, the microbial population of a subjects GI tract and/or appendix is sampled and sequenced to identify and determine the population percentage of species. The beneficial species are used to inoculate novel, distributed fermentation systems. The beneficial species are cultivated to a high concentration and reintroduced into the subjects GI tract in order to restore the gut microbiome to a balanced and healthy state. | 2021-12-09 |
20210381025 | METHODS AND KITS FOR DETECTING CELLS USING OLIGONUCLEOTIDE CONJUGATED ANTIBODIES - The tissue microenvironment is a critical factor to disease mechanism and therapeutic efficiency. Provided here is an imaging platform, methods, and kits which enable measurement of tens of parameters simultaneously within a single tissue, with the potential to reveal unique and important biological associations related to the spatial dimension. The sensitivity of the platform, methods, and kits described herein extends to measure low level markers which can be important for tracking disease progression, diagnosing disease, or both. | 2021-12-09 |
20210381026 | NOVEL IMMUNO-PCR METHOD USING cDNA DISPLAY - The present invention provides an improved immuno-PCR method by using cDNA display comprising the steps of: immobilizing a first antibody having a binding site to a solid phase: contacting a sample fluid to said antibody to bind a target molecule in said sample fluid; contacting said target molecule to a cDNA display being composed of a backbone being composed of a double strand and a side chain having a second antigen binding site to which the second antibody is bound; and conducting polymerase chain reaction to detect said cDNA quantitatively. According to the improved immune-PCR method of the present invention, the target molecule is screened and obtained quantitatively, because it uses cDNA display being composed of one protein/peptide and one DNA. | 2021-12-09 |
20210381027 | BARCODING OF NUCLEIC ACIDS - The present disclosure relates to methods, kits and products for barcoding of nucleic acids. In certain embodiments, the present disclosure provides a method of producing nucleic acid for sequencing utilising clonal amplification on a solid substrate, the method comprising: (a) providing a nucleic acid sample for sequencing; (b) amplifying the nucleic acid sample using an amplifying primer comprising a degenerate nucleotide sequence and a 5′ fixed nucleotide sequence, to produce amplified nucleic acids; and (c) further amplifying the amplified nucleic acids with (i) a first primer comprising the 5′ fixed nucleotide sequence and a first adapter nucleotide sequence, and (ii) a second primer comprising the 5′ fixed nucleotide sequence and a second adapter nucleotide sequence, wherein the first adapter nucleotide sequence or the second adapter nucleotide sequence provides a sequence for subsequent priming of DNA synthesis from a nucleotide sequence attached to the solid substrate and the other adapter nucleotide sequence provides a sequence for subsequent priming of DNA synthesis from a template produced from the subsequent priming, and wherein one or more of the first primer, the second primer and the amplifying primer comprise a specific identifier sequence to identify nucleic acids amplified with the first primer, the second primer and/or the amplifying primer; thereby producing nucleic acid for sequencing utilising clonal amplification on the solid substrate. | 2021-12-09 |
20210381028 | Methods for Detection and Quantification of Infectious Carbapenem Resistant Enterobacteriaceae (CRE) - Provided herein are qPCR-based methods for quantitatively detecting and differentiating between live and dead pathogenic bacteria such as carbapenem-resistant Enterobacteriaceae (CRE). Also provided herein are simple, fast, and reliable genomic DNA extraction methods with improved efficiency. | 2021-12-09 |
20210381029 | DOUBLE-FUNCTIONAL OLIGONUCLEOTIDE COMPRISING COMPLEMENTARY NUCLEOTIDE SEQUENCE, MIS-MATCHED NUCLEOTIDE SEQUENCE, REPORTER, AND QUENCHER, AND A METHODS FOR NUCLEIC ACID AMPLIFICATION AND MEASUREMENT USING THE SAME - The present disclosure relates to a complementary double-stranded oligo, in which, for the amplification of a particular gene sequence, an inosine linker is linked to the 5′-terminus of a primer for the corresponding sequence, a sequence complementary to the primer is linked to the inosine linker, and at least one mis-matched nucleotide is included in the complementary sequence to form a bubble structure; in which, depending on the treatment temperature, at a predetermined temperature or lower, a single stranded oligo is turned into a double-stranded form to exist in an inactivation form, and at a predetermined temperature or higher, the oligo is activated into a single-stranded oligo; and in which, a fluorescent substance (reporter dye) and a quenching material (quencher molecule) are attached to the oligo, so that the oligo can be applied as a primer or a probe, and thus only two oligos can realize the gene amplification and fluorescent signal real-time measurement with high specificity, and to a measuring method after a fluorescent arrangement step using the oligomer is added. The present disclosure has advantages in view of an oligo and a design method therefor, in which an oligo capable of simultaneously performing amplification and detection with an amplification size of 500 bp to 1000 bp for a particular gene is used to allow a reporter dye and a quencher molecule to be attached to only two target gene amplification sites, thereby confirming whether real-time amplification occurs. | 2021-12-09 |
20210381030 | SYSTEMS AND METHODS OF DIAGNOSING AND CHARACTERIZING INFECTIONS - Embodiments of the invention include methods of identifying microorganisms and/or diagnosing infections in subjects cause by microorganisms. Embodiments of the invention may also include further characterizing (e.g., determining the presence of one or more antibiotic resistance markers) the microorganisms and determining a strain identity of the microorganisms. | 2021-12-09 |
20210381031 | PRE-AMPLIFICATION ASSAY - Provided herein are methods and compositions to determine the efficacy of a nucleic acid pre-amplification reaction. | 2021-12-09 |
20210381032 | Rapid PCR Methodology - Disclosed is an enhanced method for rapid and cost-effective analysis of sequences of a microorganism by qPCR. These methods identify allelic variation, SNPs, and genetic mutations of a particular gene such as those responsible for conferring resistance or sensitivity to an antibiotic, chemotherapy, or another chemical compound. By selection of appropriate gene regions, mutation loci that confer resistance to key antibiotics can be identified by qPCR. Additionally, the approach can identify heteroresistant strains, e.g., populations of strains from a sample that contain both mutation and wild-type nucleotides. By selecting appropriate that bind efficiently to the area of mutation can identify resistance conferring mutations. Methods are useful to sequences derived from viral agents, such as influenza virus, bacterial agents, such as tuberculosis bacteria, and cancer cells. | 2021-12-09 |
20210381033 | Methods of Detecting Listeria from an Environmental Sample - The present invention relates to the identification of microorganisms from an environmental sample, and in particular to the rapid identification of | 2021-12-09 |
20210381034 | METHOD OF DETECTING RNA - The present invention relates to a method of analyzing and detecting RNA. In particular, the present invention is capable of analyzing RNA with short base sequences while quantitatively detecting RNA with high sensitivity and accuracy, and thus can be widely used for diagnosis of various diseases such as infectious diseases and cancer. | 2021-12-09 |
20210381035 | Methods of Preparing and Analyzing Nucleic Acid Libraries - Detecting different mutations in the same sample is essential, especially where the sample is limited in quantity and where high-throughput methods are desired for rapid detection of mutations. Methods routinely used in the art require separate assays for detecting different mutations or mutation types (e.g. single nucleotide polymorphisms (SNPs) or copy number variations (CNVs)) in a sample. The present disclosure provides methods for detecting different mutations, such as SNPs and CNVs in the same sample. The methods described herein can be useful in preimplantation genetic testing, carrier screening, or genotyping | 2021-12-09 |
20210381036 | METHODS AND COMPOSITION FOR HIGH THROUGHPUT SINGLE MOLECULE PROTEIN DETECTION SYSTEMS - Disclosed herein are highly multiplexed methods of detecting single target analytes, including complexes, with improved accuracy using a proximity binding assay and single molecule cycled detection. | 2021-12-09 |
20210381037 | APPARATUS AND METHOD FOR HIGH THROUGHPUT PARALLEL NUCLEIC ACID SEQUENCING ON SURFACES OF MICROBEADS - Method and apparatus for nucleic acid sequencing are provided. The method includes providing a microbead disposed in one reaction well and immobilized with two capturing oligonucleotides with different sequences, immobilizing nucleic acid templates on the microbead via annealing between the templates and the capturing oligonucleotides, amplifying the immobilized nucleic acid templates and producing a population of template clones annealed with sequencing primers. The method further includes sequentially disposing different types of nucleotide trisphosphates, detecting, by ion-sensitive field-effect transistors, ion concentration change in the reaction wells in response to incorporation of one of the nucleotide trisphosphates at 3′ end of sequencing primers, when the nucleotide trisphosphates is complementary to a corresponding nucleotide in the template clones, and sequencing the template clones by repeating the sequentially disposing and the detecting. A method for producing single-stranded nucleic acid template clones on a reaction well array is also provided. | 2021-12-09 |
20210381038 | METHOD FOR DETECTING NUCLEIC ACID - A method for detecting the presence and/or amount of a target nucleic acid molecule in a biological sample and a related kit. The method comprises: (a) contacting the biological sample with: i) Cas12b protein, ii) a gRNA targeting a target sequence of the target nucleic acid molecule, and iii) a single-stranded DNA reporter molecule generating a detectable signal after being cleaved to form a reaction mixture; and (b) detecting the presence and/or level of the detectable signal generated in the reaction mixture. | 2021-12-09 |
20210381039 | METHODS AND COMPOSITIONS FOR PATHOGEN DETECTION IN PLANTS - The technology relates in part to methods and compositions for detecting one or more pathogens in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in | 2021-12-09 |
20210381040 | METHODS FOR CHEMICAL LIGATION OF NUCLEIC ACIDS - Provided herein are methods for preparing nucleic acid libraries, capturing DNA obtained from a limited number of cells, and selectively cleaving ssDNA or dsDNA using various chemical ligation and click chemistry reactions described herein. | 2021-12-09 |
20210381041 | Enzymatic Enrichment of DNA-Pore-Polymerase Complexes - The present invention provides a method for isolating Sequencing complexes, said method comprising forming a complex between a nanopore covalently linked to a polymerase and an oligonucleotide that is associated with a purification moiety, separating any unbound/uncomplexed nanopores and oligonucleotides from the complexes by use of a solid support capable of binding the purification moiety, and cleaving bound complexes from the solid support with an enzyme composition. | 2021-12-09 |
20210381042 | Methods for Adding Adapters to Nucleic Acids and Compositions for Practicing the Same - Provided are methods of adding adapters to nucleic acids. The methods include combining in a reaction mixture a template nucleic acid, a template switch oligonucleotide, a polymerase, and dNTPs. The reaction mixture components are combined under conditions sufficient to produce a product nucleic acid that includes the template nucleic acid and the template switch oligonucleotide each hybridized to adjacent regions of a single product nucleic acid including a region polymerized from the dNTPs by the polymerase. The methods further include attaching sequencing platform adapter constructs to ends of the product nucleic acid or a derivative thereof. Aspects of the invention further include compositions and kits. | 2021-12-09 |
20210381043 | FOUR-COLOR DNA SEQUENCING BY SYNTHESIS USING CLEAVABLE FLUORESCENT NUCLEOTIDE REVERSIBLE TERMINATORS - This invention provides a process for sequencing single-stranded DNA by employing a nanopore and modified nucleotides. | 2021-12-09 |
20210381044 | METHOD OF SEQUENCING IMMOBILIZED POLYNUCLEOTIDES - The present invention concerns a method of sequencing immobilized polynucleotides in which beads which are tethered to the solid support are used as labels to identify bases within the polynucleotides. The beads carry sets of probes or bases which can bind to the polynucleotide allowing identification of the target base(s). Identification of the base(s) is achieved through sequential application of different cleavage means specific to different probes/bases carried on the beads. Also provided is an apparatus for performing the method and a kit comprising the apparatus and other components necessary for performing the method. | 2021-12-09 |
20210381045 | Single Cell Nucleic Acid Detection and Analysis - Methods and compositions for digital profiling of nucleic acid sequences present in a sample are provided. | 2021-12-09 |
20210381046 | USE OF TITANIUM NITRIDE AS AN ELECTRODE IN NON-FARADAIC ELECTROCHEMICAL CELL - A nanopore cell includes a conductive layer. The nanopore cell further includes a titanium nitride (TiN) working electrode disposed above the conductive layer. The nanopore cell further includes insulating walls disposed above the TiN working electrode, wherein the insulating walls and the TiN working electrode form a well into which an electrolyte may be contained. In some embodiments, the TiN working electrode comprises a spongy and porous TiN working electrode that is deposited by a deposition technique with conditions tuned to deposit sparsely-spaced TiN columnar structures or columns of TiN crystals above the conductive layer. | 2021-12-09 |
20210381047 | CIRCULATING MICRORNAS IN KNEE OSTEOARTHRITIS AND USES THEREOF - A method staging of knee osteoarthritis (OA) comprising
| 2021-12-09 |
20210381048 | METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING - Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. This method uniquely capitalizes on the redundant information stored in double-stranded DNA, thus overcoming technical limitations of prior methods utilizing data from only one of the two strands. | 2021-12-09 |
20210381049 | Hybridization Chain Reaction Methods for In Situ Molecular Detection - The disclosure provides a method for detecting a target analyte in a biological sample including contacting the sample with one or more probe sets each comprising a primary probe and a linker, contacting the sample with an initiator sequence, contacting the sample with a plurality of fluorescent DNA hairpins, wherein the probe binds the target molecule, the linker connects the probe to the initiator sequence, and wherein the initiator sequence nucleates with the cognate hairpin and triggers self-assembly of tethered fluorescent amplification polymers, and detecting the target molecule by measuring fluorescent signal of the sample. | 2021-12-09 |
20210381050 | METHODS FOR DIAGNOSING INFECTIOUS DISEASE AND DETERMINING HLA STATUS USING IMMUNE REPERTOIRE SEQUENCING - Methods are provided for predicting a subject's infection status using high-throughput T cell receptor sequencing to match the subject's TCR repertoire to a known set of disease-associated T cell receptor sequences. The methods of the present invention may be used to predict the status of several infectious agents in a single sample from a subject. Methods are also provided for predicting a subject's HLA status using high-throughput immune receptor sequencing. | 2021-12-09 |
20210381051 | DNA METHYLATION BIOMARKER OF AGING FOR HUMAN EX VIVO AND IN VIVO STUDIES - DNA methylation (DNAm) based biomarkers of aging have been developed for many tissues and organs. However, these biomarkers have sub-optimal accuracy in skin cells, fibroblasts and other cell types that are often used in ex vivo studies. To address this challenge, we analyzed DNA methylation array data sets derived from multiple sources of DNA, from which we developed a novel and highly robust DNAm age estimator (based on 391 CpGs) for human fibroblasts, keratinocytes, buccal cells, endothelial cells, lymphoblastoid cells, skin, blood, and saliva samples. The application of this new age estimator to ex vivo cell culture systems revealed that cellular population doubling is generally accompanied by an increase in epigenetic aging. The new skin & blood clock disclosed herein is useful for ex vivo and in vivo studies of human aging. | 2021-12-09 |
20210381052 | METHODS FOR ASSESSING RISK OF DEVELOPING A VIRAL DISEASE USING A GENETIC TEST - This document provides methods and materials related to treating a disease. For example, this document provides methods for treating a subject's disease based on identifying the risk of progressive multifocal leukoencephalopathy PML using a genetic test. | 2021-12-09 |
20210381053 | IN VITRO PHARMACOGENOMIC SCREENING METHOD FOR ANTICIPATING A PATIENT'S RESPONSE TO THE TREATMENT OF OCULAR HYPERTENSION - In vitro pharmacogenomic screening method for anticipating a patient's response to the treatment of ocular hypertension | 2021-12-09 |
20210381054 | METHODS, SYSTEMS AND KITS FOR PREDICTING PREMATURE BIRTH CONDITION - Methods and systems ( | 2021-12-09 |
20210381055 | METHODS OF DETECTING AND TREATING MULTIPLE SYSTEM ATROPHY - The present disclosure relates to a method for diagnosing and treating multiple-system atrophy (MSA) in a subject, the method comprising: determining in a subject-derived biological sample an expression level of a gene from the group consisting of: QKI, GGCX, MOCS1, NF1, LINC01572, PRRG3, HMBOX1, PLP1, PPP1CA, C8orf88, TGFB2, MASP1, TIAM1, SYNGAP1, ACTN1, EMP1, NFIL3, GPNMB, PGAM2, ST5, STON1, RFTN1, and MMP14; comparing the subject-derived expression level of the gene with a normal control expression level of the gene obtained from a non-neurodegenerative biological sample; diagnosing the subject as a having MSA by detecting a differential expression of the gene in the subject-derived biological sample as compared to the normal control expression level; and administering a peroxisome proliferator-activated receptor β (PPARβ) agonist or a retinoid X receptor (RXR) agonist to the subject diagnosed as having MSA. | 2021-12-09 |
20210381056 | SYSTEMS AND METHODS FOR JOINT INTERACTIVE VISUALIZATION OF GENE EXPRESSION AND DNA CHROMATIN ACCESSIBILITY - Systems and methods for visualizing patterns in discrete attribute value datasets are provided. A dataset comprises a discrete attribute value for each gene in a plurality of genes, for each cell in a plurality of cells. The dataset further comprises ATAC counts for each ATAC peak in a plurality of peaks, for each of the cells. Cells are assigned cluster groups in a first plurality of cluster groups based on a first clustering of discrete attribute values for the genes across the cells. Cell are also assigned cluster groups in a second plurality of cluster groups based on a second clustering of ATAC fragment count values for the ATAC peaks across the cells. A projection of the cells uses one of the first or second cluster group assignments. There is indicated, for each cell within the projection, membership in the other of the first or second cluster group assignments. | 2021-12-09 |
20210381057 | RECURRENCE GENE SIGNATURE ACROSS MULTIPLE CANCER TYPES - The present disclosure provides gene expression profiles that are associated with cancer, including certain gene expression profiles that differentiate between cancer that is at a high risk of recurrence. The gene expression profiles can be measured at the nucleic acid or protein level. The gene expression profiles can also be used to identify a subject for cancer treatment. Also provided are kits for use in predicting cancer recurrence and/or prognosing cancer and an array comprising probes for detecting the unique gene expression profiles associated with cancer. | 2021-12-09 |
20210381058 | EVIDENCE BASED SELECTION OF PATIENTS FOR CLINICAL TRIALS USING HISTOPATHOLOGY - An immune gene expression signature and immune cell distribution in the tumor, in combination, can be used to infer an immune phenotype of the tumor, which further can be used to characterize the tumor, selecting an optimal immune therapy to the tumor, and predicting the treatment outcome of an immune therapy. | 2021-12-09 |
20210381059 | USE OF LONG NON-CODING RNA FOR THE DIAGNOSIS OF PROSTATE CANCER - The present invention provides long noncoding RNAs (lncRNAs) that allow to diagnose prostate cancer much more accurately than the existent non-invasive diagnostic tools. So, this invention relates to the use of at least one of these lncRNA or a combination thereof as a diagnosis marker for prostate cancer. It also relates to an in vitro method for prostate cancer diagnosis of a subject as well as to a kit for performing this method. | 2021-12-09 |
20210381060 | CANCER SPECIFIC IMMUNOTHERAPEUTIC TARGETS GENERATED BY CHEMOTHERAPEUTIC DRUG TREATMENT - Provided are methods for identifying antigens containing amino acid sequences for use in a cancer vaccine. The vaccines and methods of use for prophylaxis and/or therapy of cancer are included. The method involves: i) exposing cancer cells to a chemotherapeutic agent that damages DNA; ii) determining open reading frames encoded by mRNA transcribed from a gene in the cancer cells of i); iii) comparing the open reading frames of the mRNA of i) to open reading frames encoded by mRNA transcribed from the gene in the cancer cells that were not exposed to the chemotherapeutic agent, iv) determining a different open reading frame encoded by the mRNA of i) and an open reading frame of the mRNA of ii), wherein the different open reading frame encoded by the mRNA of i) encodes a contiguous amino acid sequence comprising the sequence of the antigen for use in the cancer vaccine. | 2021-12-09 |
20210381061 | Immune Gene Signatures in Cancer - This invention relates to methods for selecting a treatment, treating, and predicting survival time in subjects with cancer, such as colorectal cancer, based on tumor expression levels of chemokines, cytotoxic genes, and/or dendritic cell genes. | 2021-12-09 |
20210381062 | NASAL EPITHELIUM GENE EXPRESSION SIGNATURE AND CLASSIFIER FOR THE PREDICTION OF LUNG CANCER - This application provides assays and methods for diagnosis and prognosis of lung cancer using expression analysis of one or more genes from a biological sample comprising nasal epithelial cells. The assays and methods are non-invasive and accurately detect the presence or absence of lung cancer relative to, for example, more invasive techniques, such as bronchoscopy. Similarly, the assays and methods described provide non-invasive ways of accurately identifying the smoking history of a subject. | 2021-12-09 |
20210381063 | METHODS OF TREATING CANCER PATIENTS WITH FARNESYLTRANSFERASE INHIBITORS - The present invention relates to the field of molecular biology and cancer biology. Specifically, the present invention relates to methods of treating a subject with a farnesyltransferase inhibitor (FTI) that include determining whether the subject is likely to be responsive to the FTI treatment based on HRAS mutation status in the subject. | 2021-12-09 |
20210381064 | SINGLE CELL SEQUENCING - The disclosure provides methods and systems of analyzing single cells by simultaneously separating cells into monodisperse droplets and tagging each nucleic acid molecule from the cells with barcodes unique to each droplet. The methods and systems combine template particles with a plurality of single cells in a tube, generate in the tube monodispersed droplets encapsulating a single one of the template particles and a single one of the single cells, release nucleic acid molecules from the single cells and provide each nucleic acid molecule with a barcode unique to the respective droplet. The nucleic acid molecules can then be analyzed by any known method, for example by sequencing the nucleic acid molecules. | 2021-12-09 |