48th week of 2017 patent applcation highlights part 27 |
Patent application number | Title | Published |
20170342423 | GENERAL SECRETORY PATHWAY (GSP) MUTANT LISTERIA SPP., AND METHODS FOR MAKING AND USING THE SAME - General secretory pathway (GSP) mutant | 2017-11-30 |
20170342424 | METHODS OF PRODUCING RECOMBINANT MINICIRCLE CONSTRUCTS - The present invention relates to a method for the production of a minicircle. In the method of the present invention, a parent plasmid is provided which has a nucleic sequence flanked by recombination sites. This parent plasmid is exposed to an enzyme which causes recombination at the recombination sites, thereby to form a (i) minicircle comprising the nucleic acid sequence and (ii) a miniplasmid comprising the remainder of the parent plasmid. One recombination site is modified at the 5′ end such that its reaction with the enzyme is less efficient than the wild type site, and the other recombination site is modified at the 3′ end such that its reaction with the enzyme is less efficient than the wild type site, and the other recombination site is modified at the 3′ end such that its reaction with the enzyme is less efficient than the wild type site, both modified sites being located in the minicircle after recombination. This favours the formation of minicircle. | 2017-11-30 |
20170342425 | HIGHLY EFFICIENT ETHANOL-FERMENTATIVE YEAST - Provided is a highly efficient ethanol-fermentative yeast having high efficiency in ethanol production without introducing a foreign gene. The highly efficient ethanol-fermentative yeast is a fermentative yeast that effectively produces ethanol from pentose and hexose and is deposited to NITE Patent Microorganisms Depositary under the accession number NITE BP-01962. | 2017-11-30 |
20170342426 | Methods for Enhancing Segregation of Transgenes in Plants and Compositions Thereof - The compositions and methods are provided that enhance the selection of transgenic plants having two T-DNA molecules integrated into a plant genome at different physical and genetic loci. The compositions are DNA constructs that comprise novel arrangements of T-DNA molecules containing genes of interest, positive selectable marker genes, and conditional lethal genes. The methods disclosed herein comprises transforming a plant cell to comprise the DNA constructs of the present invention, regenerating the plant cell into a plant and identifying independant transgene loci, where the selectable marker genes or transgenic elements can be segregated in the progeny. | 2017-11-30 |
20170342427 | METHOD FOR CHANGING THE INTERCELLULAR MOBILITY OF AN MRNA - The present invention relates to methods for changing the intercellular mobility of an mRNA of a gene in an organism, comprising: modifying a tRNA-like structure present in the mRNA by mutating the gene from which the mRNA is transcribed, or including the sequence of a tRNA-like structure in the transcribed part of the gene. Mutating the gene may be for inducing loss of mobility of the transcript and comprises deleting the sequence of the tRNA-like structure from the gene, mutating the sequence of the tRNA-like structure to change the tridimensional configuration thereof, or inserting a genetic element into the gene to remove the tRNA-like structure from the transcribed part of the gene, or for inducing a change in the destination of the transcript and comprises modifying the sequence of the tRNA-like structure from the gene such that the transcript is addressed to a location different from its original destination. | 2017-11-30 |
20170342428 | PLANT REGULATORY ELEMENTS AND USES THEREOF - The invention provides recombinant DNA molecules and constructs, as well as their nucleotide sequences, useful for modulating gene expression in plants. The invention also provides transgenic plants, plant cells, plant parts, and seeds comprising the recombinant DNA molecules operably linked to heterologous transcribable DNA molecules, as are methods of their use. | 2017-11-30 |
20170342429 | PLANT PROTECTION FROM A PEST OR PATHOGEN BY EXPRESSION OF DOUBLE-STRANDED RNAs IN THE PLASTID - The present invention lies in the field of plant protection, in particular in the field of controlling plant pests and pathogens that affect plants. The present invention relates to a plant comprising a plastid comprising a double-stranded RNA (dsRNA) capable of silencing at least one target gene of a pest of a plant or of an agent causing a disease of a plant. The present invention further relates to such a transplastomic plant, wherein said dsRNA comprises two (separate) complementary single-stranded RNA strands. The present invention further relates to a plastid as comprised in the plant of the invention and to a plant cell comprising said plastid. Moreover, the present invention relates to a method of producing a plant of the invention and to a method of controlling a pest of a plant or a plant disease-causing agent or of protecting a plant from said pest or agent. Furthermore, the present invention relates to the use of a dsRNA for controlling a pest of a plant or a plant disease-causing agent or for protecting a plant from said pest or agent. | 2017-11-30 |
20170342430 | DOMINANT GENE SUPPRESSION TRANSGENES AND METHODS OF USING SAME - Pairs of plants are provided in which complementing constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants and methods of use of said plants, are provided, including use of parental plants to produce sterile plants for hybrid seed production. Also provided are methods for maintaining a homozygous recessive condition and for repressing transmission of transgenes. | 2017-11-30 |
20170342431 | METHODS AND COMPOSITIONS FOR THE INTRODUCTION AND REGULATED EXPRESSION OF GENES IN PLANTS - Compositions and methods are provided for the introduction and the regulated expression of genes in plants. Compositions include promoter constructs that provide a level of activity useful for the regulated expression of site-specific recombinases, while avoiding premature excision. Further provided are isolated polynucleotides encoding novel babyboom polypeptides, expression cassettes, and plants comprising the same. Methods for the introduction of genes into plants are provided, including methods for plastid transformation and methods for the transformation of tissues from mature seeds and leaves. | 2017-11-30 |
20170342432 | POLYSACCHARIDE SYNTHASES - The present invention relates generally to polysaccharide synthases. More particularly, the present invention relates to (1,3;1,4)-β- | 2017-11-30 |
20170342433 | Expression of Microbial Proteins in Plants for Production of Plants with Improved Properties - Recombinant constructs and methods useful for improvement of plants are provided. In particular, recombinant constructs comprising promoters functional in plant cells positioned for expression of polynucleotides encoding polypeptides from microbial sources are provided. The disclosed constructs and methods find use in production of transgenic plants to provide plants, particularly crop plants, having improved properties. | 2017-11-30 |
20170342434 | Chloroplast Expressing Colostrum or Milk Polypeptides - Provided are chloroplasts engineered to recombinantly express mammalian colostrum and milk polypeptides photosynthetic organisms containing such chloroplasts, and compositions comprising such organisms and methods for producing such organisms. In certain embodiments, provided is a chloroplast comprising one or more polynucleotides encoding one or more mammalian milk or colostrum polypeptides selected from osteopontin, lactadherin, cathelicidin-1, lysozyme, lactoperoxidase, lingual antimicrobial peptide (LAP), alpha-lactalbumin, and soluble CD14. | 2017-11-30 |
20170342435 | ISOLATED POLYPEPTIDES AND POLYNUCLEOTIDES USEFUL FOR INCREASING NITROGEN USE EFFICIENCY, ABIOTIC STRESS TOLERANCE, YIELD AND BIOMASS IN PLANTS - Provided are methods of increasing nitrogen use efficiency, fertilizer use efficiency, yield, growth rate, vigor, biomass, oil content and/or abiotic stress tolerance of a plant by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence encoding a polypeptide at least 80% identical to SEQ ID NO: 2560, 2557, 184, 238, 188, 154-156, 158-161, 163-183, 185-187, 189-197, 200-237, 239-264, 266-269, 1351, 1365-1425, 1429-1457, 1459, 1461-1730, 1735, 1739-2397, 2533-2541, 2544-2556, 2558, 2559, 2561-2562 or 2563. Also provided are isolated polynucleotides and polypeptides which can be used to increase nitrogen use efficiency, fertilizer use efficiency, yield, growth rate, vigor, biomass, oil content and/or abiotic stress tolerance of a plant of a plant. | 2017-11-30 |
20170342436 | TRANSGENIC PLANTS EXHIBITING ENHANCED PHYTOCHELATIN-BASED HEAVY METAL TOLERANCE AND METHODS OF USE THEREOF - Transgenic plants exhibiting phytochelatin-based heavy metal tolerance and methods of use thereof for bioremediation are disclosed. | 2017-11-30 |
20170342437 | Genes and uses for plant improvement - This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits. | 2017-11-30 |
20170342438 | THRIPS RESISTANT CABBAGE - The invention relates to a | 2017-11-30 |
20170342439 | METHOD FOR EXTRACTING DIFFERENTIATED CELLS - A method for extracting differentiated cells from a cell population comprising undifferentiated cells after induction of the differentiation of pluripotent stem cells. A method for extracting differentiated cells from a cell population, comprising the following steps: (1) a step of introducing, into a cell population, mRNA comprising a marker gene operably linked to the target sequence of miRNA specifically expressed in pluripotent stem cells; and (2) a step of extracting cells in which the marker gene has been translated. | 2017-11-30 |
20170342440 | Mutant Receptors and Their Use in a Nuclear Receptor-Based Inducible Gene Expression System - This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms. | 2017-11-30 |
20170342441 | Hepatocyte Based Insulin Gene Therapy for Diabetes - A method and vectors for controlling blood glucose levels in a mammal are disclosed. In one embodiment, the method comprises the steps of: treating the hepatocyte cells of a patient with a first, second or third vector, wherein the first vector comprises a promoter enhancer, glucose inducible regulatory elements, a liver-specific promoter, a gene encoding human insulin with modified peptidase and an albumin 3′UTR and lacks an HGH intron, wherein the second vector comprises an HGH intron, glucose inducible regulatory elements, a liver-specific promoter, a gene encoding human insulin with modified peptidase site and an albumin 3′UTR and lacks a promoter enhancer, wherein the third vector comprises an HGH intron, glucose inducible regulatory elements, a liver-specific promoter, a gene encoding human insulin with modified peptidase site, an albumin 3′UTR and a promoter enhancer and observing the patient's insulin levels, wherein the patient's insulin levels are controlled. | 2017-11-30 |
20170342442 | RECOMBINANT SELF-REPLICATING POLYCISTRONIC RNA MOLECULES - This disclosure provides recombinant polycistronic nucleic acid molecules that contain at at least four nucleotide sequences that encode a protein of interest, particularly proteins that form complexes in vivo, each operably linked to a separate subgenomic promoter. In some embodiments these proteins and the complexes they form elicit potent neutralizing antibodies. Thus, presentation of herpes virus proteins using the disclosed platforms permits the generation of broad and potent immune responses useful for vaccine development. | 2017-11-30 |
20170342443 | PROCESS FOR ENZYMATIC HYDROLYSIS OF LIGNOCELLULOSIC MATERIAL AND FERMENTATION OF SUGARS - The invention relates to an integrated process for alcohol production from lignocellulosic material. | 2017-11-30 |
20170342444 | SYSTEMS AND METHODS FOR CONTINUOUSLY FERMENTING C5 AND C6 SACCHARIDES - This invention provides optimized fermentation of cellulosic and hemicellulosic sugars. Biomass-derived hemicellulosic and cellulosic sugars are independently conditioned and separately fermented, utilizing reuse and recycle of microorganisms, metabolic intermediates, and nutrients. Conditioned sugars can be fermented in separate vessels, where excess cells from glucose fermentation are conveyed to hemicellulose sugar fermentation along with raffinate from solvent recovery, to enhance productivity and product yield. Some variations provide a method of fermenting C | 2017-11-30 |
20170342445 | Integration of a Polynucleotide Encoding a Polypeptide That Catalyzes Pyruvate to Acetolactate Conversion - The invention relates to recombinant host cells having at least one integrated polynucleotide encoding a polypeptide that catalyzes a step in a pyruvate-utilizing biosynthetic pathway, e.g., pyruvate to acetolactate conversion. The invention also relates to methods of increasing the biosynthetic production of isobutanol, 2,3-butanediol, 2-butanol or 2-butanone using such host cells. | 2017-11-30 |
20170342446 | SYSTEM AND METHOD FOR CONTROLLING METABOLITE PRODUCTION IN A MICROBIAL FERMENTATION - A method is provided for controlling a metabolic profile of an anaerobic microbial fermentation culture. In particular, a metabolic profile of a fermentation process is controlled by controlling the amount of dissolved CO | 2017-11-30 |
20170342447 | METHODS OF AND SYSTEMS FOR PRODUCING CAPRYLIC ACID AND/OR CAPRYLATE - Methods and systems to produce product compositions comprising caprylate products using chain-elongating bacteria. For example, the caprylate product in the product composition is n-caprylic acid (C8) and the n-caprylic (C8) to n-caproic (C6) acid ratio is higher than 1:1. These methods use chain elongation towards C8 rather than C6. High n-caprylate productivity and specificity was accomplished by: 1) feeding a substrate with, for example, ethanol as the carbon source or alternatively, a high ethanol-to-acetate ratio as the carbon source; 2) extracting caprylate product(s) (e.g., n-caprylate product) from the bioreactor broth; and 3) acclimating an efficient chain-elongating microbiome. The methods can produce caprylate products such as, for example, n-caprylic acid, which is a higher value chemical than C4 and C6. | 2017-11-30 |
20170342448 | Method for increasing Lipid content in Microorganisms and modified Microorganisms therefrom - The present disclosure relates to a method for increasing lipid content in microorganisms. The method comprises decreasing the expression of molecules involved in the protein synthesis to decrease protein synthesis and thereby increase lipid synthesis in the microorganisms. The present disclosure also provides a modified microorganism having increased lipid content. | 2017-11-30 |
20170342449 | Production System and Method of Production for Product Selected from Nitrogen-Containing Product and Fermented and Cultured Product - Provided is a novel production system for a product selected from a nitrogen-containing product and a fermented and cultured product that does not involve (or can minimize) the transport of liquid ammonia. A production system for a product selected from a nitrogen-containing product and a fermented and cultured product can include: an ammonia synthesis apparatus in which an ammonia-containing gas is synthesized by reaction of a source gas containing hydrogen and nitrogen in the presence of a supported metal catalyst containing as a support one or more selected from the group consisting of: i) a conductive mayenite compound; ii) a two-dimensional electride compound or a precursor thereof; and iii) a complex formed of a support base containing at least one metal oxide selected from ZrO | 2017-11-30 |
20170342450 | Production System and Method of Production for Organic Compound or Microorganism - Provided is a novel production system that does not involve, or can minimize, the transport of liquid ammonia in the production of an organic compound or the production of a microorganism by microbial fermentation. A production system for an organic compound or a microorganism includes: an ammonia synthesis apparatus in which an ammonia-containing gas is synthesized by reaction of a source gas containing hydrogen and nitrogen in the presence of a supported ruthenium catalyst; and a culture apparatus that cultures a microorganism having organic compound productivity using ammonia originating from the ammonia-containing gas obtained by using the ammonia synthesis apparatus. | 2017-11-30 |
20170342451 | BIOSYNTHESIS OF PACLITAXEL INTERMEDIATE - The invention relates to methods of making compounds useful for production of paclitaxel and analogs or derivatives thereof. | 2017-11-30 |
20170342452 | Mutant microorganisms to synthesize colanic acid, mannosylated and/or fucosylated oligosaccharides - The present invention relates to mutated and/or transformed microorganisms for the synthesis of various compounds. More specifically, the present invention discloses microorganisms mutated in the genes encoding for the regulators ArcA and IclR. The latter mutations result in a significant upregulation of the genes that are part of the colanic acid operon. Hence, said microorganisms are useful for the synthesis of any compound being part of the colanic acid pathway such as GDP-fucose, GDP-mannose and colanic acid, and/or, can be further used—starting form GDP-fucose as a precursor—to synthesize fucosylated oligosaccharides or—starting from GDP-mannose as a precursor—to synthesize mannosylated oligosaccharides. In addition, mutations in the genes coding for the transcriptional regulators ArcA and IclR lead to an acid resistance phenotype in the exponential growth phase allowing the synthesis of pH sensitive molecules or organic acids. | 2017-11-30 |
20170342453 | CHITIN AND CHITOSAN PRODUCING METHODS - The invention relates to the field of polymer production, in particular to the production of chitin and chitosan from microalgae belonging to the phylum Haptophyta to the phylum Chlorophyta, or to the phylum Heterokontophyta, particularly from microalgae of the genus | 2017-11-30 |
20170342454 | METHOD FOR PREPARING RECOMBINANT PROTEINS THROUGH REDUCTION OF rnpA GENE EXPRESSION - The present invention relates to a method for improving the production of a difficult-to-express recombinant protein in a recombinant microorganism, and more particularly to a method for improving the production of a difficult-to-express recombinant protein by use of a recombinant microorganism into which a gene encoding a target protein and an sRNA against a gene encoding ribonuclease P are introduced. According to the present invention, expressions of a large recombinant protein, a difficult-to-express protein and a useful protein can be dramatically increased by reducing expression of the rnpA gene. | 2017-11-30 |
20170342455 | METHODS AND APPARATUS FOR ASSAYS OF BACTERIAL SPORES - Described herein are methods and apparatus for assays of bacterial spores. In particular, methods and apparatus for lateral flow immunoassay for bacterial spore detection and quantification, live/dead assay for bacterial spores, lifetime-gated measurements of bacterial spores and imaging bacterial spores using an active pixel sensor, and unattended monitoring of bacterial spores in the air are described. | 2017-11-30 |
20170342456 | METHOD OF DETECTING INVASIVE FUNGI ACCORDING TO MORPHOLOGY THEREOF BASED ON CONTRAST STAINING, AND KIT FOR SAME - A method of detecting invasive fungi according to morphology thereof based on contrast staining, including: sterilizing and storing the necessary equipment aseptically; drawing 1 ml of venous blood from a tested subject's elbow vein; dripping one drop of the venous blood, prior to coagulation, into an ampoule containing 0.8 ml of a detection reagent under an aseptic environment; gently shaking the ampoule until the drop of venous blood is evenly distributed; leaving the ampoule to stand for 20 minutes to form a stained solution; sterilizing or disinfecting a microscope slide and a cover slip; dripping one drop of the stained solution on the microscope slide prepared under aseptic condition; observing the sample sequentially with 4×, 10× and 40× objective lenses and a 100× oil-immersion lens; magnifying with a 5 million pixel eyepiece; displaying an image of the sample on computer screen using a high-resolution imaging software for observation and record. | 2017-11-30 |
20170342457 | METHODS, APPARATUSES, AND SYSTEMS FOR ANALYZING MICROORGANISM STRAINS FROM COMPLEX HETEROGENEOUS COMMUNITIES, PREDICTING AND IDENTIFYING FUNCTIONAL RELATIONSHIPS AND INTERACTIONS THEREOF, AND SELECTING AND SYNTHESIZING MICROBIAL ENSEMBLES BASED THEREON - Methods, apparatuses, and systems for screening, analyzing and selecting microorganisms from complex heterogeneous communities, predicting and identifying functional relationships and interactions thereof, and synthesizing microbial ensembles based thereon are disclosed. Methods for identifying and determining the absolute cell count of microorganism types and strains, along with identifying the network relationships between active microorganisms and environmental parameters, are also disclosed. | 2017-11-30 |
20170342458 | BIOMASS CONTAINMENT DEVICE - A biomass containment device (BCD) and methods of measuring microbial growth or enzyme activity in the presence of insoluble substrates using the BCD is described. The BCD is compatible with microbial growth and enzyme assays, is sterilizable, is reusable, and the size can be varied to fit any container. | 2017-11-30 |
20170342459 | DEVICES, SYSTEMS AND METHODS TO DETECT VIABLE INFECTIOUS AGENTS IN A FLUID SAMPLE AND SUSCEPTIBILITY OF INFECTIOUS AGENTS TO ANTI-INFECTIVES - Various devices, systems and methods for detecting infectious agents or determining a susceptibility of an infectious agent to an anti-infective are described herein. One example method comprises introducing a fluid sample to a surface; exposing the surface to a solution; sampling the solution after exposing the solution to the surface; and detecting a change in an electrical characteristic of a sensing device exposed to the solution sampled corresponding to a presence of the infectious agent in the fluid sample. | 2017-11-30 |
20170342460 | METHOD FOR SCREENING FOR BIOACTIVE NATURAL PRODUCTS - Describe is a method for screening mutant prokaryotic cells to identify producers of a cytotoxic agent active against a target cell, the method comprising the steps of: (a) providing cells of a producer prokaryotic species; (b) generating a pool of mutant producer cells by transposon mutagenesis of the cells of step (a) with an activating transposon (TnA), wherein the TnA comprises an outward-facing promoter (TnAP) capable of increasing transcription of a gene at or near its insertion site in the DNA of said producer cells; (c) co-encapsulating individual members of the pool of step (b) with one or more target cells in microdroplets, the microdroplets comprising a volume of aqueous growth media suspended in an immiscible carrier liquid, thereby generating a library of microdroplets each comprising a single mutant producer cell and one or more target cell(s); (d) incubating the microdroplet library of step (c) under conditions suitable for co-culture of the single mutant producer cell and target cell(s) to produce a library of microcultures, whereby mutant producer cells producing a cytotoxic agent active against the target cell(s) outgrow target cells in each microculture; and (e) screening the library of microcultures of step (d) for microcultures in which target cells have been outgrown or overgrown to extinction by mutant producer cells. | 2017-11-30 |
20170342461 | TYROSINE KINASE BIOSENSORS AND METHODS OF USE - Disclosed are compositions and methods for measuring tyrosine kinase activity. | 2017-11-30 |
20170342462 | GENOME ARCHITECTURE MAPPING - The present invention relates to the field of analysis of the three-dimensional structure of the genome, i.e., for genome architecture mapping (GAM). The invention provides a method of determining spatial proximity of a plurality of nucleic acid loci in a compartment such as the cell nucleus, by exploiting their co-segregation amongst fractions of that compartment, identified upon separation of the nucleic acid loci from each other depending on their localization in the compartment to obtain a collection of fractions, e.g., by cryo-sectioning or cryo-milling the compartment; determining the presence or absence of the plurality of loci in said fractions; and determining the co-segregation of said plurality of loci. Co-segregation may then be analysed with statistical methods to determine spatial proximity. The method can be used e.g., for determining physical distance between a plurality of loci; and mapping loci and/or genome architecture, e.g., in the nucleus; identification of regulatory regions directing expression of a specific gene through spatial contacts; identifying the nuclear position of an exogenous nucleic acid in the nucleus and/or diagnosing a disease associated with a disturbed co-segregation of loci. | 2017-11-30 |
20170342463 | DIGITAL PROXIMITY ASSAY - Methods of determining the presence or absence of a target in a sample are provided. Kits for performing the methods described herein are also provided. | 2017-11-30 |
20170342464 | PROCESSING BLOOD SAMPLES TO DETECT TARGET NUCLEIC ACIDS - Provided herein are porous polymer monolith materials and processes that enable integration of blood fractionation, specific nucleic acid amplification and/or detection of nucleic acids from whole blood. | 2017-11-30 |
20170342465 | ERROR CORRECTION IN AMPLIFICATION OF SAMPLES - Disclosed herein are methods and systems for correcting errors in sample amplification, including the errors occurred in determining the number of targets in samples. In some embodiments, the method comprises: stochastically barcoding a plurality of targets in the samples using oligonucleotides comprising stochastic barcodes to generate stochastically barcoded targets; contacting one or more defined barcoded primers with each of the one or more samples; and determining an amplification noise. | 2017-11-30 |
20170342466 | PROCESSING BLOOD SAMPLES TO DETECT TARGET NUCLEIC ACIDS - Provided herein are porous polymer monolith materials and processes that enable integration of blood fractionation, specific nucleic acid amplification and/or detection of nucleic acids from whole blood. | 2017-11-30 |
20170342467 | Methods For Isolating Nucleic Acids From Samples - Techniques and methods are provided for isolating nucleic acids from a sample. The methods include adding a chelating agent to the sample to block nucleic acid binding sites on contaminants in the sample; heating the sample to remove hydrocarbons; and lysing the cells using freeze-thaw cycles. | 2017-11-30 |
20170342468 | COMPOSITIONS AND METHODS FOR DETECTION OF MYCOPLASMA GENITALIUM - Methods for the rapid detection of the presence or absence of | 2017-11-30 |
20170342469 | CHROMATIN ACTIVITY PRECIPITATION METHOD AND SYSTEM - Methods and systems for identifying binding sites in macromolecules using small molecule mimics of naturally occurring molecules is disclosed. A reactive probe is provided that mimics small molecule cofactors. A target macromolecule is irreversibly bound to the probe in vivo to selectively pull down or precipitate probe-bound macromolecules. The macromolecules may be, but are not limited to, DNA, RNA, and proteins. | 2017-11-30 |
20170342470 | DILUENT FOR FLUORESCENT NANO PARTICLES, KIT FOR IMMUNOFLUORESCENT STAINING WHICH UTILIZES SAME, SOLUTION FOR IMMUNOFLUORESCENT STAINING, IMMUNOFLUORESCENT STAINING METHOD, AND GENE STAINING METHOD - [Problem ] Provided is a means for detecting and quantifying a biological substance of interest with an improved accuracy by inhibiting non-specific adsorption of fluorescent nanoparticles and thereby reducing the background noise in immunostaining with fluorescent nanoparticles. [Means for Solution] Immunostaining is carried out upon diluting fluorescent nanoparticles with a fluorescent nanoparticle diluent which contains 1 to 5% (W/W) of a protein having a molecular weight of 40,000 or higher (e,g., BSA) and 1 to 3% (W/W) of a protein having a molecular weight of less than 40,000 (eg ., casein) and, when casein is used as a low-molecular-weight protein, it is preferred that the κ-casein content in the casein is 10% (W/W) or less and the ratio of α-casein and β-casein (α-casein:β-casein) contained in the casein is 40:60 to 60:40 ( taking the total amount of α-casein and β-casein as 100). | 2017-11-30 |
20170342471 | Oligonucleotide Probe for Detecting Single Nucleotide Polymorphism, and Method for Detecting Single Nucleotide Polymorphism - The present invention provides an oligonucleotide probe for single nucleotide polymorphism detection to be used for a target nucleic acid where a single nucleotide polymorphism is present, the oligonucleotide probe comprising a reporter region, an anchor region, and a linker region. The reporter region comprises: an oligonucleotide consisting of a sequence perfectly matching when a nucleotide of the single nucleotide polymorphism is a first nucleotide, and mismatching when the nucleotide of the single nucleotide polymorphism is a nucleotide other than the first nucleotide; and a fluorescent dye quenching when the reporter region hybridize to the target nucleic acid. | 2017-11-30 |
20170342472 | INSTANTANEOUS DETECTION OF BIOMARKS AND USES THEREOF - The present invention relates to a novel method for detecting instantaneously a biomarker immobilized to a solid surface and related uses. The method comprises exposing the biomarker to a probe having a magnetic label in a solution; applying a magnetic field to the solution, whereby a complex of the biomarker and the probe is formed on the solid surface; withdrawing the magnetic field; removing the solution from the solid surface; and detecting the complex instantaneously, wherein the presence of the complex on the solid surface indicates the presence of the biomarker. | 2017-11-30 |
20170342473 | Recombinase Polymerase Amplification - This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes. Further, the improved processivity of the disclosed methods may allow amplification of DNA up to hundres of megabases in length. | 2017-11-30 |
20170342474 | POLYNUCLEOTIDE AMPLIFICATION USING CRISPR-CAS SYSTEMS - A method for amplifying a target nucleic acid including providing a system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid, and contacting the target nucleic acid with the system to form a complex. | 2017-11-30 |
20170342475 | COMPOSITIONS AND METHODS FOR PROCESSING AND AMPLIFICATION OF DNA, INCLUDING USING MULTIPLE ENZYMES IN A SINGLE REACTION - The present invention concerns preparation of DNA molecules, such as a library, using a stem-loop oligonucleotide. In particular embodiments, the invention employs a single reaction mixture and conditions. In particular, at least part of the inverted palindrome is removed during the preparation of the molecules to facilitate amplification of the molecules. Thus, in specific embodiments, the DNA molecules are suitable for amplification and are not hindered by the presence of the palindrome. | 2017-11-30 |
20170342476 | Hybrid Multi-Step Nucleic Acid Amplification - Improved methods for amplifying target nucleic acid sequences are provided by 1) first amplifying the number of copies of target nucleic acid sequences in a sample by a first nucleic acid amplification method, and then 2) applying a second nucleic amplification method to the amplified sample, or aliquot thereof, further amplifying the number of copies of target sequences. In embodiments, a first nucleic acid amplification method is a thermocycling method, and a second nucleic acid amplification method is an isothermal method. | 2017-11-30 |
20170342477 | Methods for Detecting Genetic Variations - Technology provided herein relates in part to methods, processes, machines and apparatuses for detecting genetic variations. In some embodiments, the technology is related to non-invasive assessment of aneuploidies. | 2017-11-30 |
20170342478 | MAGNETIC FLUX DENSITY BASED DNA SEQUENCING - In an approach to magnetic flux density based DNA sequencing, a static magnetic field is provided. A chain of nucleotides is passed through the magnetic field. A change in magnetic flux density of the static magnetic field due to an ionic voltage associated with an individual nucleotide or base pair of the chain of nucleotides is measured. An identity of the nucleotide is determined based on the change in magnetic flux density. | 2017-11-30 |
20170342479 | METHODS OF HUMAN LEUKOCYTE ANTIGEN TYPING - Described herein are methods, systems, and media for HLA typing an individual from nucleic acid or protein sequences. The methodology disclosed herein represents significant improvements over current methods of HLA typing. | 2017-11-30 |
20170342480 | METHODS AND SYSTEMS FOR NUCLEIC ACID SEQUENCING BY TUNNELING RECOGNITION - Embodiments may include a method of analyzing a nucleic acid molecule. The method may include attaching the nucleic acid molecule to a protein. The protein may be attached to a particle with a first diameter. The method may also include applying an electric field to move a first portion of the nucleic acid molecule into an aperture. The aperture may be defined by a first electrode, an insulator, and a second electrode. The aperture may have a second diameter less than the first diameter. The method may further include contacting the first portion of the nucleic acid molecule to both the first electrode and the second electrode. The method may include applying a voltage across the first electrode and the second electrode. The current through the electrodes and the portion of the nucleic acid molecule may be measured, and a nucleotide of the nucleic acid molecule may be identified. | 2017-11-30 |
20170342481 | INTERMITTENT DETECTION DURING ANALYTICAL REACTIONS - Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period. | 2017-11-30 |
20170342482 | METHODS FOR SEQUENCING A POLYNUCLEOTIDE TEMPLATE - The invention relates to methods for pairwise sequencing of a polynucleotide template which result in the sequential determination of nucleotide sequence in two distinct and separate regions of the polynucleotide template. | 2017-11-30 |
20170342483 | TRANSPOSASE-RANDOM PRIMING DNA SAMPLE PREPARATION - Provided herein, among other things, are a variety of methods for transposase-mediated tagging and amplification of short DNA fragments, e.g., between about 150 bp and 1.5 Kb in length. In some aspects, the method includes tagging the DNA fragments with a first primer sequence using barcoded transposases followed by a primer extension reaction to introduce a second primer sequence, e.g., using random or gene-specific primers. Kits for performing this method are also provided. | 2017-11-30 |
20170342484 | NORMALIZATION OF NUCLEIC ACID LIBRARIES - This disclosure provides methods and compositions for removing one or more high abundance species from a plurality of nucleic acid molecules. In some embodiments, the methods and compositions can be used for normalizing nucleic acid libraries. In some embodiments, molecular labels are used in conjunction with the methods and compositions disclosed herein to improve sequencing efficiency. | 2017-11-30 |
20170342485 | TAGGED MULTI-NUCLEOTIDES USEFUL FOR NUCLEIC ACID SEQUENCING - The present disclosure relates to tagged multi-nucleotide compounds, which comprise a single tag moiety covalently linked to a plurality of nucleoside-5′-oligophosphate moieties. As disclosed herein, these tagged multi-nucleotide compounds have improved characteristics as polymerase substrates and can be used in a range of nucleic acid detection and sequencing methods, including nanopore sequencing-by-synthesis. | 2017-11-30 |
20170342486 | HIGH THROUGHPUT SCREENING OF POPULATIONS CARRYING NATURALLY OCCURRING MUTATIONS - Efficient methods are disclosed for the high throughput identification of mutations in genes in members of mutagenized populations. The methods comprise DNA isolation, pooling, amplification, creation of libraries, high throughput sequencing of libraries, preferably by sequencing-by-synthesis technologies, identification of mutations and identification of the member of the population carrying the mutation and identification of the mutation. | 2017-11-30 |
20170342487 | POLYMER COATINGS - The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein. | 2017-11-30 |
20170342488 | METHODS OF USING FGF19 MODULATORS - Provided herein are methods of using FGF19 modulators and/or bile acid metabolism biomarkers. | 2017-11-30 |
20170342489 | Attribute Sieving and Profiling with Sample Enrichment by Optimized Pooling - A process of identifying a plurality of biological samples having particular desired attributes by testing pooled samples and selecting, for intended uses such as transfusion, or for subsequent analysis that is thereby enriched for such samples, pooled samples which have, or may have, said desired attributes. The preferred number of samples per pool “d” is determined by selecting an integer value as d which produces the maximum or a value near the maximum of the product of: d times the expected number of unambiguous sample pools, where a sample pool is unambiguous if all of the samples have the desired attributes, and is otherwise ambiguous if at least one sample has the desired attributes. The value selected as d can be greater than the maximum product above, so as to enlarge the total number of samples assayed in determining the desired attributes. | 2017-11-30 |
20170342490 | METHOD FOR DETERMINING STATE OF DIFFERENTIATION OF STEM CELLS, AND NOVEL DIFFERENTIATION MARKER USED THEREFOR - The invention relates to a method which is capable of determining, at an initial stage of differentiation, the differentiated state of undifferentiated stem cells. The invention provides a method for determining a differentiated state of a cell comprising detecting expression of FOXB2 gene of a stem cell, and determining the differentiated state based on the result, and a differentiation marker selected from mRNA or protein derived from the FOXB2 gene. The invention is applicable to quality management of the stem cells or to methods for preparation and isolation of the differentiated cells. Further, because the differentiated cells can be determined at an initial stage of culture, the invention is useful for an early stage screening of cells and for quality management of the stem cells, and reduction in culturing period and cost reduction in expenditure regarding a culture medium, or the like, can be expected. | 2017-11-30 |
20170342491 | METHODS AND COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION - Compositions that are used in nucleic acid amplification in vitro are disclosed, which include a target specific universal (TSU) promoter primer or promoter provider oligonucleotide that includes a target specific (TS) sequence that hybridizes specifically to a target sequence that is amplified and a universal (U) sequence that is introduced into the sequence that is amplified, by using a primer for the universal sequence. Methods of nucleic acid amplification in vitro are disclosed that use one or more TSU oligonucleotides to attached a U sequence to a target nucleic acid in a target capture step and then use a primer for a U sequence in subsequent amplification steps performed in substantially isothermal conditions to make amplification products that contain a U sequence that indicates the presence of the target nucleic acid in a sample. | 2017-11-30 |
20170342492 | DIAGNOSTIC ASSAY FOR TISSUE TRANSPLANTATION STATUS - A method of detecting circulatory nucleic acids from a transplanted tissue in a transplant recipient, the method comprising: amplifying circulatory nucleic acids from a transplanted tissue in a blood sample from the transplant recipient, and detecting amplification of a copy number deletion (CND) polymorphism from the transplanted tissue, and related methods for determining the status of a donor tissue transplanted into a recipient. | 2017-11-30 |
20170342493 | THERAPY SELECTION FOR PSORIASIS AND PSORIATIC ARTHRITIS - The present invention provides methods for selecting an individual with psoriasis (Ps) or psoriatic arthritis (PsA) who should receive or who is likely to respond to a treatment with an anti-tumor necrosis factor alpha (anti-TNFα) therapy. In addition, provided herein are methods for selecting an individual with Ps or PsA who should receive or who is likely to respond to a therapy that is not an anti-TNFα therapy, e.g., a non-anti-TNFα therapy for the treatment of Ps or PsA. Specifically, the methods of the present invention relate to detecting the presence of distinct alleles of the PDE3A-SLCO1C1 locus which are associated with a clinical response to an anti-TNFα therapy or a non-anti-TNF therapy in patients with Ps or PsA. | 2017-11-30 |
20170342494 | METHODS FOR PREDICTING GRAFT ALTERATIONS - The invention relates to a method for predicting graft alterations in a transplanted patient, comprising a step of determining the expression levels of bactericidal/permeability-increasing protein (BPI), chemokine (C motif) ligand 1 (XCL1) and thioredoxin domain containing 3 (TXNDC3) genes in a biological sample obtained from said transplanted patient. | 2017-11-30 |
20170342495 | MARKERS OF PRETERM BIRTH - The invention relates to novel markers of preterm birth, methods for assessing the status of preterm birth using the markers, and methods for the diagnosis and therapy of preterm birth. | 2017-11-30 |
20170342496 | METHODS FOR EVALUATING, MONITORING, AND MODULATING AGING PROCESS - Disclosed herein are methods of increasing the expression rate of epigenetic markers such as ELOVL2, KLF14, and PENK with administration of a therapeutic agent (e.g., vitamin C or its derivatives, analogs, metabolites, prodrugs, or pharmaceutically acceptable salts thereof). Also described herein are methods of modulating the methylation pattern of epigenetic markers such as ELOVL2, KLF14, and PENK with administration of a therapeutic agent (e.g., vitamin C or its derivatives, analogs, metabolites, prodrugs, or pharmaceutically acceptable salts thereof). | 2017-11-30 |
20170342497 | Kits and Methods for Monitoring Therapy and/or for Adapting Therapy of an Epithelial Cancer Patient - The present invention relates to kits and methods for monitoring therapy and/or for adapting therapy of an epithelial cancer patient, and for determining malignancy grade or progression of a tumor of a patient suffering from an epithelial tumor. | 2017-11-30 |
20170342498 | PARP GENOMIC VARIANTS CONFERRING RESISTANCE AND SENSITIZATION TO CHEMOTHERAPY UNDER INHIBITION - A method is provided for selecting a subject diagnosed with cancer as a candidate for treatment with a PARP1 inhibitor, or both a PARP1 inhibitor and a chemotherapeutic agent such as an alkylating agent. The method includes detecting the presence or absence of a mutation in a non-coding region of a PARP1 gene, wherein the presence of a mutation in the PARP1 gene indicates that the cancer can be treated with the PARP1 inhibitor, optionally in conjunction with the chemotherapeutic agent. In a specific, non-limiting example, the PARP1 inhibitor is verliparib (ABT-888). | 2017-11-30 |
20170342499 | GENOMIC CLASSIFIER THAT PREDICTS RESPONSE TO MULTI-KINASE INHIBITOR TREATMENT INTRODUCTION - The method for predicting the anti-tumor response in a human or animal having a tumor to multiple kinase inhibitors, using any multiple kinase inhibitor, comprises selection of genes encoding for protein kinases targeted by the said tyrosine kinase inhibitor, for each one of these genes, providing at least one nucleic acid probe which hybridizes to said gene under stringent conditions, thus providing an array of nucleic acid probes, having a biological sample containing cancer cells from said human or animal, extracting DNA from the sample, fragmenting into DNA fragments, optionally labeling the DNA fragments, submitting the optionally labeled DNA fragments to hybridization with the array of nucleic acid probes, recovering and quantifying for all the genes the gains or losses in gene copy numbers, wherein gains and losses of gene copy numbers of each selected gene are used to determine whether the tumor is sensitive or not to said kinase inhibitor. | 2017-11-30 |
20170342500 | METHOD FOR IDENTIFICATION OF TISSUE OR ORGAN LOCALIZATION OF A TUMOUR - The invention relates to a method for predicting the localization of a primary tumour, wherein said method comprises the use of genomic profile data, and wherein the method is capable of predicting the type of cancer by a classification score ranking among a variety of the possible tumour types. | 2017-11-30 |
20170342501 | USE OF GENE EXPRESSION PROFILING TO PREDICT SURVIVAL IN CANCER PATIENT - Gene expression profiling in multiple myeloma patients identifies genes that distinguish between patients with subsequent early death or long survival after treatment. Poor survival is linked to over-expression of genes such as ASPM, OPN3 and CKS1B which are located in chromosome 1q. Given the frequent amplification of 1q in many cancers, it is possible that these genes can be used as powerful prognostic markers and therapeutic targets for multiple myeloma and other cancer. | 2017-11-30 |
20170342502 | BIOMARKERS FOR BREAST CANCER AND METHODS OF USE THEREOF - Described herein is the use of Agouti Signaling Protein (ASIP), in addition to certain other melanocortin signaling network (MSN) genes, as prognostic and predictive biomarkers for the progression of breast cancer. In particular, the novel biomarkers can be used to determine if a female breast cancer patient is at risk of progressing to metastatic disease and thus also be used to direct treatment of the patient. | 2017-11-30 |
20170342503 | XRN2 AS A DETERMINANT OF SENSITIVITY TO DNA DAMAGE - The present disclosure is directed to methods of detecting XRN2 expression levels, copy number and mutation status, particularly in cancer cells. The methods permit physicians to tailor therapies to subject having certain genotypes/phenotypes, and to exclude therapies unlikely to be effective. | 2017-11-30 |
20170342504 | EARLY OVARIAN CANCER DETECTION DIAGNOSTIC TEST BASED ON MRNA ISOFORMS - A method to detect ovarian cancer is provided that employs probes and/or primers to detect certain RNA isoform transcripts, as well as kits therefor. | 2017-11-30 |
20170342505 | Diagnosing and Monitoring CNS Malignancies Using MicroRNA - The use of specific microRNAs (miRNAs) present in CSF as biomarkers for particular brain malignancies and disease activity. | 2017-11-30 |
20170342506 | DEVICE, SYSTEM AND METHOD FOR CANCER PROGNOSIS AND USES THEREOF - Modules, devices, systems and methods for measuring or detecting cysteine and/or methionine metabolite levels in a sample from a subject are disclosed. Various embodiments of the present invention concern modules, devices, systems and methods for prognosing or diagnosing cancer, for example, prostate, colon, ovarian or breast cancer; predicting the risk or probability of cancer recurrence; and/or for predicting, detecting and/or monitoring cystinuria or cystine stone disease. | 2017-11-30 |
20170342507 | METHODS AND MATERIALS FOR DETERMINING THE SOURCE OF WASTE - A method for identifying the source of animal waste is provided. The method includes taking DNA samples from a known group of animals, conducting DNA analysis on the DNA samples to prepare a genetic profile for each animal from the group, preparing a database of the genetic profiles, collecting a specimen of waste from an unknown source, conducting DNA analysis on the specimen, and comparing the DNA analysis from the specimen to the database to determine the source of the waste. | 2017-11-30 |
20170342508 | COMPOSITIONS AND METHODS FOR DETECTION OF TRICHOMONAS VAGINALIS - Methods for the rapid detection of the presence or absence of | 2017-11-30 |
20170342509 | GENETIC LOCI ASSOCIATED WITH FUSARIUM SOLANI TOLERANCE IN SOYBEAN - The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to | 2017-11-30 |
20170342510 | PROCESS FOR THE PREPARATION OF A SACCHARIDE-CONTAINING SOLUTION FROM A TORREFIED CELLULOSIC BIOMASS - A saccharide-containing solution is prepared from a cellulosic biomass feedstock in a process, where the cellulosic biomass feedstock includes torrefied biomass material, and where the torrefied biomass material is hydrolyzed in an aqueous reactant by contacting it with the aqueous reactant that comprises hydrochloric acid with a concentration of 35 to 45% wt, based on the weight of the combination of hydrochloric acid and water, to yield a saccharide-containing solution. | 2017-11-30 |
20170342511 | PROCESS FOR THE ISOLATION OF MONOSACCHARIDES - A process for the separation of a monosaccharide from an aqueous solution comprising the monosaccharide, in particular a hydrolysate of a polysaccharide containing biomass, characterized in that a) the solution comprises one or more salts or mineral acids, b) the solution is contacted with a zeolite adsorbent preferably of BEA zeotype for adsorbing the monosaccharide on the zeolite, c) the zeolite with the adsorbed monosaccharide is separated from the solution, d) the monosaccharide is separated from the zeolite absorbent. The process in a chromatographic process, in particular SMB, produces relatively highly concentrated and pure monosaccharide solution in water. | 2017-11-30 |
20170342512 | Dispersing Agent Composition for Tanning of Hides/Skins and Process for the Preparation Thereof - Disclosed herein is a dispersing agent composition essentially comprising polyalkylene polyhydric compound, an organic acid and a non-ionic surfactant. The dispersing composition finds application in leather processing industry for tanning without adding any water or any other medium. It finds tremendous application potential in the tanning industry to ensure eco-benign leather processing that does not add to environmental pollution as no effluent is generated. The invention is therefore envisaged to play a crucial role in enhancing the economic and environmental benefits associated with tanning industry. The disclosure also relates to a process for preparing the composition and also the process of eco-benign tanning using the said composition. | 2017-11-30 |
20170342513 | Method of Sealing and Repairing a Refractory Tap Hole - A method of sealing a slag drain in a direct smelting vessel is disclosed. Also disclosed are a method of maintaining a slag drain channel and a direct smelting vessel with a slag drain channel that extends through a sleeve of refractory material installed in the direct smelting vessel. The method for sealing the slag drain includes locating a pre-formed refractory material at an inlet end of the slag drain channel so that it is exposed to a molten bath contained within the direct smelting vessel and sealing the slag drain channel with sealing material downstream of the pre-formed refractory material. | 2017-11-30 |
20170342514 | METHOD FOR SMELTING NICKEL OXIDE ORE - Provided is a smelting method capable of effectively promoting a reduction reaction on pellets formed using nickel oxide ore as starting material to obtain a ferronickel alloy with a high nickel grade of at least 4%. The present invention is a method for smelting nickel oxide ore wherein ferronickel alloy with a nickel grade of at least 4%, the method comprising a pellet-producing step S | 2017-11-30 |
20170342515 | METHODS FOR MANGANESE REMOVAL OF CAST IRON - The present invention does not require a demanganese agent such as a sulfide or a combustible gas in the removal of manganese of cast iron. The method for removing manganese of cast iron according to the present invention is implemented by performing the removal of a manganese component by allowing a furnace to be in an oxygen atmosphere, and by blowing air into a molten cast iron in the furnace, while a carbon component in the molten cast iron is being maintained at an approximately constant amount. Alternatively, the method for removing manganese of cast iron according to the present invention is implemented by performing the removal of the manganese component by allowing the furnace to be in an oxygen atmosphere and by stirring the molten cast iron in the furnace, while the carbon component in the molten cast iron is being maintained at an approximately constant amount. | 2017-11-30 |
20170342516 | Sandwich Structure and Associated Pressure-based Forming Method - A sandwich structure forming method including the steps of (1) providing a sandwich structure comprising a core positioned between a first liner sheet and a second liner sheet; (2) positioning the sandwich structure into a cavity of a die assembly; and (3) pressurizing the core to expand the sandwich structure into engagement with the die assembly. | 2017-11-30 |
20170342517 | HOT-WORKING TOOL AND MANUFACTURING METHOD THEREFOR - Provided is a hot-working tool capable of maintaining adequate toughness even if the permissible amount of P contained in the hot-working tool is increased. The present invention is a hot-working tool, which has a component composition that can be adjusted to a martensitic structure by quenching and has a post-quenching and tempering martensitic structure, wherein: the component composition comprises greater than 0.020 mass % to 0.050 mass % of P; prior austenite grain diameter in said post-quenching and tempering martensitic structure is at least No. 9.5 in grain size number according to JIS-G-0551; and the P concentration of the grain boundary of said prior austenite particles is not more than 1.5 mass %. A hot-working tool wherein said component composition also comprises not more than 0.0250 mass % of Zn is preferable. The present invention also is a method for manufacturing a hot-working tool in which quenching and tempering are performed on a hot-working tool material with said component composition. | 2017-11-30 |
20170342518 | STRUCTURAL ULTRA-THICK STEEL HAVING EXCELLENT RESISTANCE TO BRITTLE CRACK PROPAGATION, AND PRODUCTION METHOD THEREFOR - Provided are structural ultra-thick steel having excellent resistance to brittle crack propagation and a production method therefor. The structural ultra-thick steel comprises 0.02-0.1 wt % of C, 0.8-2.5 wt % of Mn, 0.05-1.5 wt % of Ni, 0.005-0.1 wt % of Nb, and 0.005-0.1 wt % of Ti with the remainder being Fe and other inevitable impurities, and has microstructures including one structure selected from the group consisting of a single-phase structure of ferrite, a single-phase structure of bainite, a complex-phase structure of ferrite and bainite, a complex-phase structure of ferrite and pearlite, and a complex-phase structure of ferrite, bainite, and pearlite. The ultra-thick structural steel has excellent resistance to brittle crack propagation, excellent yield strength and an excellent impact transition temperature in the center. | 2017-11-30 |
20170342519 | MATERIAL FOR LAMINATED IRON CORE, AND METHOD OF MANUFACTURING LAMINATED IRON CORE - A material for laminated iron cores is used as plural steel sheets to be overlapped with one another and punched when a laminated iron core is manufactured. A surface roughness of the steel sheets forming the material for laminated iron cores is at an arithmetic mean roughness Ra of 0.40 [μm] or less, and a sheet thickness deviation in a sheet width direction of at least a portion used as the laminated iron core is 3 [μm] or less per 500 [mm], the portion being of the steel sheets forming the material for laminated iron cores. | 2017-11-30 |
20170342520 | METHOD FOR HEAT TREATMENT OF AUSTENITIC STEELS AND AUSTENITIC STEELS OBTAINED THEREBY - The invention concerns a method for heat treatment of an austenitic steel of the High Nitrogen Steel or austenitic HNS type, or of an austenitic steel of the High Interstitial Steel or austenitic HIS type, said austenitic HNS or austenitic HIS containing precipitates of nitrides, carbides or carbonitrides of chromium and/or of molybdenum, this method comprising the step which consists, after machining the austenitic HNS or austenitic HIS containing the precipitates, in redissolving the precipitates by bringing the austenitic HNS or austenitic HIS to its austenitizing temperature, then cooling the austenitic HNS or austenitic HIS sufficiently rapidly to avoid the re-formation of precipitates. | 2017-11-30 |
20170342521 | ALLOY STEEL COMPOSITION AND PRODUCIING METHOD THEREOF - A method for producing an alloy steel composition includes the following steps: performing a first heat treatment on an alloy steel composition and maintaining for a first time period to soften the alloy steel composition; performing a first cooling treatment on the softened alloy steel composition; performing a treatment on the softened the alloy steel composition to form a workpiece; performing a second heat treatment on the workpiece and maintaining for a second time period; and performing a second cooling treatment on the workpiece to make the workpiece become to be a Bainite structure, and a cooling rate of the second cooling treatment is high than the cooling rate of the first cooling treatment. | 2017-11-30 |
20170342522 | HOT-ROLLED STEEL SHEET FOR HIGH STRENGTH GALVANIZED STEEL SHEET, HAVING EXCELLENT SURFACE QUALITY, AND METHOD FOR PRODUCING SAME - The present invention relates to a hot-rolled steel sheet for a high strength galvanized steel sheet, having excellent surface quality, and a method for producing the same, the hot-rolled steel sheet comprising, by weight %: C: 0.05 to 0.15%, Si: 0.03 to 0.10%, Mn: 0.7 to 1.39%, P: 0.001 to 0.05%, S: 0.001 to 0.03%, Al: 0.002 to 0.035%, and the remainder being Fe and unavoidable impurities. The weight ratio of Mn/Si is 15 to 25, the weight ratio of C/Si is 1 to 5, and the weight ratio of Si/P is 3 to 10. The hot-rolled steel sheet has a microstructure consisting of, in area fraction, 10 to 40% of bainite, 20 to 30% of pearlite and 40 to 60% of ferrite, and includes a ternary eutectic compound of FeO, Fe | 2017-11-30 |