46th week of 2013 patent applcation highlights part 46 |
Patent application number | Title | Published |
20130302805 | METHODS FOR REMOVAL OF SPECIFIC SEED TISSUE OR STRUCTURE FOR SEED ANALYSIS - A method for reducing resources for selecting seed to be produced in commercial quantities or for research is disclosed. Samples of seed which are candidates for selection are collected and given an identifier. Specific tissue or structure from candidate seed is removed. A test or analysis is performed on the candidate seed or the removed tissue or structure. Results of the test or analysis are recorded and correlated to the seed's identifier. The results are evaluated and a decision is made whether to select a candidate seed for commercial production or for research. Time, space, and labor associated with growing plants in an experimental plot or greenhouse and taking tissue samples from growing plants is saved. | 2013-11-14 |
20130302806 | MODULATION OF EXON RECOGNITION IN PRE-MRNA BY INTERFERING WITH THE SECONDARY RNA STRUCTURE - The invention relates to oligonucleotides for inducing skipping of exon 55 of the dystrophin gene. The invention also relates to methods of inducing exon 55 skipping using the oligonucleotides. | 2013-11-14 |
20130302807 | METHODS, SYSTEMS, AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS - Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases. | 2013-11-14 |
20130302808 | PREDICTING CANCER OUTCOME - This document provides methods and materials related to assessing prostate cancer in mammals. For example, methods and materials for using ERG expression levels and TOP2A expression levels to identify mammals (e.g., male human patients) as being susceptible to a poor prostate cancer outcome are provided. | 2013-11-14 |
20130302809 | Plurality of Reaction Chambers in a Test Cartridge - A fluidic testing system is presented that includes a plurality of test chambers, a plurality of inlet channels, and a fluidic network that connects the inlet channels to one or more other chambers. The plurality of test chambers are each characterized by a length and a hydraulic diameter. The length of each test chamber is aligned substantially parallel to a gravity vector. Each of the test chambers has only one opening disposed along the length of the corresponding test chamber. Additionally, each of the test chambers is coupled via its respective opening to only one of the plurality of inlet channels. | 2013-11-14 |
20130302810 | MODIFIED SURFACES AS SOLID SUPPORTS FOR NUCLEIC ACID PURIFICATION - The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle. | 2013-11-14 |
20130302811 | SYSTEM FOR DIAGNOSING AVELLINO CORNEAL DYSTROPHY - The present invention relates to a system for diagnosing Avellino corneal dystrophy, and more particularly to a system for diagnosing Avellino corneal dystrophy, in which whether a sample is normal or Avellino corneal dystrophy is determined based on the ratio of the input first PCR amplification value and the second PCR amplification value. The system makes it possible to diagnosis Avellino corneal dystrophy in a simpler and accurate manner without being influenced by the doctor's skill. Particularly, the inventive system makes the overall process systematic, and thus provides accurate diagnosis. In addition, the system can also easily administer a number of test subjects. | 2013-11-14 |
20130302812 | METHOD OF MICROALGAL CHLOROPLAST TRANSFORMATION USING FUNCTIONAL SELECTION IN THE ABSENCE OF ANTIBIOTICS - This invention relates to a new method of selecting for transgene transformants in the absence of antibiotic selective pressure, where the method is based on recovery of function. | 2013-11-14 |
20130302813 | CELL WALL PROTEIN CwpV (CD0514) AS A DIAGNOSTIC MARKER FOR CLOSTRIDIUM DIFFICILE RIBOTYPE 027 - Accurate and rapid differentiation of the outbreak strain ribotype 027 from other possible | 2013-11-14 |
20130302814 | PROCESS FOR ISOLATING MICROORGANISMS - A process for isolating microorganisms is disclosed. The process utilizes a device comprising an inner surface, an outer surface, a first port, and a second port, wherein the inner surface comprises an unmodified, smooth glass substrate and defines a binding chamber providing fluid communication between the first port and second port. Microorganisms in an aqueous solution are contacted with the unmodified, smooth glass substrate, wherein the solution is essentially free of cell precipitants, and the microorganisms are allowed to bind to the glass substrate. | 2013-11-14 |
20130302815 | Screening Method - The invention provides a polynucleotide comprising a reporter sequence operatively linked to a regulatory element of a gene selected from Bscl2, Srxnl, Cbr3, Ephxl, Nope, Cdknla, Perp, Pltp, Cgrefl, Ltb4r1, Btg2, Gpx2, Ltb4r2, Ddit4l, Fosl1, and Egr1, which regulatory element stimulates expression of the reporter sequence in response to a genotoxic agent or to an oxidative stress-inducing agent. The invention also provides a method of detecting a genotoxic or oxidative stress-inducing agent comprising subjecting a cell containing the polynucleotide of the invention to a test agent; and assessing the expression of the reporter sequence. The invention provides a method of detecting a genotoxic or oxidative stress-inducing agent comprising subjecting one or more cells that comprise a reporter sequence operatively linked to a regulatory element of a gene, which regulatory element stimulates expression of the reporter sequence in response to a genotoxic agent or to an oxidative stress-inducing agent, to a test agent, and assessing the expression of the one or more reporter sequences; wherein at least one cell subjected to a test agent comprises a polynucleotide comprising a reporter sequence operatively linked to a regulatory element of the Bsc12 gene. | 2013-11-14 |
20130302816 | NUCLEAR DNA CONTENT OF CHO CELL LINES AS A SELECTION CRITERION IN SCREENING OF THE BEST RECOMBINANT PROTEIN PRODUCERS - The present invention relates to a method for selecting for cells or cell lines that produce a recombinant protein/polypeptide in high yields, the method allowing for the selection of high producer cells or cell lines in an early phase of cell line development, the method comprising the step of determining the nuclear DNA content of the cells or cell lines, wherein the level of the nuclear DNA content of the cells or cell lines positively correlates with the capacity of the cells or cell lines to produce the recombinant protein/polypeptide. | 2013-11-14 |
20130302817 | Functionalized Microfluidic Device And Method - A microfluidic device and method is provided for handheld diagnostics and assays. The device includes a base having outer surface and a channel therethough for receiving fluid therein. The channel has input and output ports communicating with the outer surface. A lid is also provided. The lid has an outer surface, a first well having a port communicating with the outer surface of the lid, and a second well having a port communicating with the outer surface. The lid moveable between a first disengaged position and a second engaged position wherein the first port of the lid is adjacent the input port of the channel and the second port is adjacent the output port of the channel. | 2013-11-14 |
20130302818 | IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD - An automated microscope slide staining system and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments or a pressurizable common chamber for individually and independently processing a plurality of microscope slides. The apparatus preferably features independently movable slide support elements each having an individually operable heating element. | 2013-11-14 |
20130302819 | METHOD FOR THE EARLY IDENTIFICATION AND PREDICTION OF AN ABRUPT REDUCTION IN KIDNEY FUNCTION IN A PATIENT UNDERGOING CARDIOTHORACIC SURGERY - A method for the early identification and prediction of abrupt reduction in kidney function in a patient undergoing cardiothoracic (CT) surgery, including Cardio-Pulmonary Bypass (CPB), comprises contacting a urine sample from the patient with a capture molecule for a biomarker, especially πGST specific for the distal region of the renal tubule and which biomarker is released from said region when there is damage to said region indicative and predictive of an abrupt reduction in kidney function, the biomarker being detectable as early as intraoperatively or in the recovery stage post CT surgery, for example prior to transfer of the patient to the Intensive Care Unit (ICU), allowing for immediate corrective medical intervention. The method can be used to detect Acute Kidney Injury (AKI) and a requirement for Renal Replacement Therapy (RRT) namely dialysis, earlier than two hours post CT surgery and as early as zero hours post or during CT surgery or CPB. | 2013-11-14 |
20130302820 | Methods and Compositions for Treating Bleeding Disorders - Aspects of the invention include methods for enhancing blood coagulation in a subject. In practicing methods according to certain embodiments, an amount of a non-anticoagulant sulfated polysaccharide (NASP) is administered to a subject to enhance blood coagulation in the subject. Also provided are methods for preparing a NASP composition having blood coagulation enhancing activity. Compositions and kits for practicing methods of the invention are also described. | 2013-11-14 |
20130302821 | Method For Evaluating Atherosclerotic Lesion, And Kit - The present invention provides a method and a means for evaluating atherosclerotic lesions by identifying a protein (marker protein) group, the expression level of which varies according to the progression of the atherosclerotic lesion, and using the proteins. Specifically, the present invention relates to a method for evaluating atherosclerotic lesions, comprising the steps of detecting a marker protein exhibiting an expression pattern (expression variation) characteristic at a specific disease stage of atherosclerotic lesions in a subject, and evaluating the atherosclerotic lesions in the subject based on the detection result. | 2013-11-14 |
20130302822 | METHODS OF ANALYZING EXOSOMES USING FLUORESCENCE-LABELED EXOSOMES - A method of analyzing exosomes, the method comprising incubating a sample comprising fluorescence-labeled exosomes with a solid support so that the fluorescence-labeled exosomes bind to the solid support; measuring a fluorescence signal from the bound fluorescence-labeled exosomes; and analyzing the exosomes based on the measured fluorescence signal. | 2013-11-14 |
20130302823 | T1R HETERO-OLIGOMERIC TASTE RECEPTORS AND CELL LINES THAT EXPRESS SAID RECEPTORS AND USE THEREOF FOR IDENTIFICATION OF TASTE COMPOUNDS - The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R 1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. | 2013-11-14 |
20130302824 | METHODS FOR DETECTION OF RARE SUBPOPULATIONS OF CELLS AND HIGHLY PURIFIED COMPOSITIONS OF CELLS - Methods are provided for detection of a target cell type within a cell population, and compositions are provided comprising cells and an indicator that indicates the number of cells of the target cell type in the cell population. Examples are provided in which these methods are used to detect human embryonic stem cells within a differentiated cell population with exquisite sensitivity. Differentiated cells produced from embryonic stem cells can be characterized by these methods before transplantation into a recipient, thereby providing further assurance of safety. | 2013-11-14 |
20130302825 | USES OF MACROPHAGE MANNOSE RECEPTOR TO SCREEN COMPOUNDS AND USES OF THESE COMPOUNDS - Methods and associated compositions of matter (e.g., kits, cell lines, etc.) for screening compounds that bind to macrophase mannose receptor (MMR). Compounds identified by these methods and drug conjugates that includes these compounds are also encompassed as are their uses in the manufacture of medicaments. | 2013-11-14 |
20130302826 | METHOD FOR QUANTIFICATION OF SOLUBLE LR 11 - Provided is a method for quantifying soluble LR11 in a biological sample such as serum by an immunological means conveniently and accurately without the need of carrying out any complicated separation manipulation. An immunological quantification method for soluble LR11 in a sample derived from a mammal, including a step of treating the sample with at least one surfactant selected from a group consisting of a polyoxyalkylene alkyl ether, a polyoxyalkylene alkyl phenyl ether, an alkyl glycoside, an alkylthio glycoside, an acyl-N-methylglucamide and a salt of cholic acid. | 2013-11-14 |
20130302827 | ANNEXIN-BASED APOPTOSIS MARKERS - The present invention describes an annexin derivative and a method of using the annexin derivative as a biosensor for real-time visualization of phosphatidylserine exposure, apoptosis, live-cell imaging and monitoring of cell health. | 2013-11-14 |
20130302828 | METHOD FOR DETECTING LOW CONCENTRATIONS OF SPECIFIC CELL FROM HIGH CONCENTRATIONS OF CELL POPULATIONS, AND METHOD FOR COLLECTING AND ANALYZING DETECTED CELL - Conventional CTC detection methods have been problematic in that 1) there is no technique for automatically determining and counting live CTCs in a brief period of time, 2) no process has been developed for detecting, counting, and thereafter collecting and culturing live CTCs, and 3) there exists no flow cytometer that is contamination free and is capable of measuring an entire sample. Provided is a CTC detection method which comprises a pre-treatment step for concentrating and fluorescence staining CTCs, and a step for identifying and counting CTCs. The pre-treatment step includes attaching magnetic beads to EpCAM antibodies expressed by epithelial cell-derived CTCs and concentrating the CTCs through the use of a magnet, fluorescently labeling an epithelia cell surface marker of the CTCs through the use of EpCAM antibodies or 5E11 antibodies, and performing two types of nuclear staining, one being cell membrane-permeable and the other being cell membrane-impermeable. The identifying and counting step includes evaluating the respective absolute concentrations of live and dead CTCs in a volume of blood by automatically identifying CTCs by the ratio of a plurality of fluorescence signal intensities using a flow cytometer, and differentiating between and counting the live CTCs and the dead CTCs. In the cytometer, an entire liquid-feeding system that includes a flow cell can be replaced for each sample, and the total amount of a liquid sample can be measured. | 2013-11-14 |
20130302829 | METHOD FOR PREDICTING THE RESPONSE TO HER2-DIRECTED THERAPY - This invention provides methods for determining or predicting response to HER2-directed therapy in an individual. | 2013-11-14 |
20130302830 | ROTORS FOR IMMUNOASSAYS - The present invention provides an analyte detection system for detecting target analytes in a sample. In particular, the invention provides a detection system in a rotor or disc format that utilizes a centrifugal force to move the sample through the detection system. Methods of using the rotor detection system to detect analytes in samples, particularly biological samples, and kits comprising the rotor detection system are also disclosed. | 2013-11-14 |
20130302831 | METHODS AND KITS FOR DETECTING AND DIAGNOSING NEUROTRAUMA - Methods and kits for detecting and diagnosing neurotrauma (e.g., traumatic brain injury, stroke, or spinal cord injury) are provided. These methods rely on the determination of lysophosphatidic acid (LPA) and/or LPA metabolite levels in patient samples following suspected injury. | 2013-11-14 |
20130302832 | METHOD FOR ELECTRICAL DETECTION OF BIOMOLECULES BY METAL DISSOLUTION AND ASSAY KIT THEREFORE - The present invention relates to a method for detecting a target molecule in a sample by metal dissolution as described in the specification, said method using an electrical readout; to kits adapted for this method and to electrodes suitable for such kits and methods. | 2013-11-14 |
20130302833 | METHOD FOR CONTROL OF INVASION AND USE OF ADIPONECTIN - Physical condition under stress can be controlled by using adiponectin in blood as a stress marker and measuring a level thereof. Moreover, endotoxin activity can be inhibited by using adiponectin. | 2013-11-14 |
20130302834 | METHOD FOR EVALUATING REGENERATED CARTILAGE - A method, according to an embodiment, for evaluating a regenerated cartilage includes allowing a group of cells containing auricular chondrocytes to stand in the presence of a culture medium, subsequently collecting at least a portion of a liquid component from the culture medium, measuring the GFAP content of the collected liquid component, and determining whether a regenerated cartilage that has been obtained or can be obtained from the group of cells is suitable for transplantation based on the GFAP content. | 2013-11-14 |
20130302835 | ANTIBODIES DIRECTED AGAINST PYROGLUTAMATE MONOCYTE CHEMOATTRACTANT PROTEIN-1 (MCP-1 N1pE) - Monoclonal antibodies which bind specifically to the proinflammatory cytokine pyroglutamate MCP-1 (MCP-1 N1pE). | 2013-11-14 |
20130302836 | COMMON LIGHT CHAIN MOUSE - A genetically modified mouse is provided, wherein the mouse is incapable of rearranging and expressing an endogenous mouse immunoglobulin light chain variable sequence, wherein the mouse expresses only one or two human light chain variable domains encoded by human immunoglobulin sequences operably linked to the mouse kappa (κ) constant gene at the endogenous mouse κ locus, wherein the mouse expresses a reverse chimeric antibody having a light chain variable domain derived from one of only two human light chain variable region gene segments and a mouse κ constant domain, and a human heavy chain variable domain and a mouse heavy chain constant domain, from an endogenous mouse heavy chain locus. Bispecific epitope-binding proteins that are fully human are provided, comprising two different heavy chains that associate with an identical light chain that comprises a variable domain derived from one of two different human light chain variable region gene segments. | 2013-11-14 |
20130302837 | Immunoassay for Direct Determination of Antigen Content of Products Comprising Adjuvant-Coupled-Antigen Particles - The present invention relates to methods for the direct determination of the antigen content of products comprising adjuvant-coupled-antigen particles. Specifically, the present invention relates to a method for determining the antigen content of a product comprising antigen-coupled-adjuvant particles, the method comprises the steps of: a) contacting the product comprising antigen-coupled-adjuvant particles with immunoglobulin molecules capable of recognizing the antigen under conditions allowing antigen-immunoglobulin binding; b) providing a surface with the antigen, without the adjuvant, immobilized thereon; c) contacting the immunoglobulin contacted product of step (a) with the surface of step (b) under conditions allowing antigen-immunoglobulin binding; d) removing non-bound immunoglobulin molecules and antigen-coupled-adjuvant particles bound immunoglobulin molecules; e) detecting antigen-bound immunoglobulin molecules thereby determining the antigen content of a product comprising antigen-coupled-adjuvant particles. | 2013-11-14 |
20130302838 | BIOMARKER FOR AMYLOID-B-RELATED NEUROLOGICAL DISORDERS - A novel biomarker for diagnosing amyloid β-related neuropathy such as Alzheimer's disease is provided. Disclosed is a method for diagnosing Alzheimer's disease comprising measuring the level of a MFG-E8 protein in a blood, serum or plasma sample obtained from a human or animal subject, wherein the measured value is used as a marker for diagnosis of Alzheimer's disease. The MFG-E8 protein is found to bind to phosphatidylserine in a Ca | 2013-11-14 |
20130302839 | ANALYSIS OF DIRECT FACTOR Xa INHIBITORS - The present invention relates to a method for detecting at least one direct factor Xa inhibitor in a sample other than citrate plasma, comprising the step of mixing a sample containing a factor Xa inhibitor with a composition containing factor Xa under conditions which allow the factor Xa to release a detectable substance from a chromogenic substrate. | 2013-11-14 |
20130302840 | BLOOD ALCOHOL TEST APPARATUS AND METHOD - The subject matter consists of a small, hand held, portable, battery run device similar to a current diabetic monitor. The device will be carried on a person in a custom protective case that can be attached to a belt, in a pocket or on the waist of a shirt or pants. The monitor test strip will be impregnated with a substance that will be able to detect, with only a small amount of blood (e.g. a drop or a few drops) the BAC of an individual within a relatively short amount of time (e.g. seconds to minutes). | 2013-11-14 |
20130302841 | PROGNOSIS AND RISK ASSESSMENT OF PATIENTS WITH NON-SPECIFIC COMPLAINTS - The present invention relates to the determination of the level of marker peptides in a sample derived from a bodily fluid of a subject presenting to the emergency department with non-specific complaints. | 2013-11-14 |
20130302842 | Lid For Functionalized Microfluidic Platform And Method - A microfluidic device and method is provided for handheld diagnostics and assays. A first substance is frozen in a cryopreservation fluid in a first well of a lid. The lid includes a first surface communicating with a first port of the first well and a second surface communicating with a second port of the first well. A porous membrane is affixed to the first surface so as to overlap the first port and a non-porous membrane is affixed to the second surface so as to overlap the second port. The first substance may be dialytically freed from the cryopreservation fluid at a user desired time. Thereafter, the lid may be moved from a first position wherein the lid is spaced from a base to a second position wherein the lid is adjacent the channel in the base such that the first substance communicates with the input of the channel. | 2013-11-14 |
20130302843 | METHOD OF MEASURING BIOLOGICAL SAMPLE PROPERTIES AND BIOLOGICAL SAMPLE PROPERTY MEASURING APPARATUS - A method of measuring biological sample properties and a biological sample property measuring apparatus is provided. A method of measuring biological sample properties includes disposing a biomaterial to contact a sensing unit, detecting a radio frequency (RF) signal flowing through the sensing unit, and obtaining an RF property indicator of the biomaterial based on the detected RF signal. | 2013-11-14 |
20130302844 | COMPOSITION FOR PREVENTING INFLAMMATIONS - The purpose of the present invention is to provide a probiotic(s) which can activate an aromatic hydrocarbon receptor (AhR) and can consequently prevent inflammatory damage in the digestive tract. The present invention relates to; a probiotic(s) capable of activating an AhR; an anti-inflammatory agent comprising the probiotic(s); an orally ingestible composition containing the anti-inflammatory agent; and a method for screening for the probiotic(s). | 2013-11-14 |
20130302845 | MEASURING BIOLOGICAL TISSUE - A tissue measurement system includes a clamp, a measuring head, and a deflection sensor. The clamp includes a first plate and a second plate releasably engageable with one another to secure a substantially planar biological tissue. In the engaged position, the first and second plates define at least a portion of a test section along which a portion of the secured substantially planar biological tissue is disposed. The measuring head is movable toward the test section and into contact with the portion of the substantially planar biological tissue disposed along the test section. The deflection sensor is responsive to movement of the biological tissue contacted by the measuring head. | 2013-11-14 |
20130302846 | Pluripotent cells from mammalian late epiblast layer - The invention relates to the isolation and propagation of pluripotent cells isolated from the mammalian late epiblast layer, termed Epiblast Stem Cells (EpiSCs). These cells are useful in a range of applications, including the generation of transgenic animal species. | 2013-11-14 |
20130302847 | METHOD AND SAMPLE SUPPORT TO ASSIST THE MANUAL PREPARATION OF SAMPLES FOR IONIZATION WITH MATRIX-ASSISTED LASER DESORPTION - The invention relates to a method to assist with the manual preparation of a sample support for ionization with matrix-assisted laser desorption where a sample support with sample sites is provided, a selected sample site is highlighted in a way which can be perceived by the human eye at least with respect to neighboring, not selected sample sites, a sample is manually deposited on the selected and highlighted sample site, and the deposition state of at least the selected and highlighted sample site is determined. The method enables the sample preparation and sample analysis to be made more efficient and more certain. | 2013-11-14 |
20130302848 | DEVICE AND METHOD OF TAKING SAMPLES FROM MEDICAL EQUIPMENT FOR THE PURPOSE OF MICROBIOLOGICAL TESTING - A device and method for use in taking a sample from an item of medical equipment for the purpose of microbiological testing. The device includes attachment mechanism for attaching about a region of the item of medical equipment in an air-tight manner; and collection mechanism for collecting fluid which emanates from the item of medical equipment. | 2013-11-14 |
20130302849 | BIOLOGICAL STERILIZATION INDICATOR AND METHOD OF USING SAME - A biological sterilization indicator (BI) and method of using same. The BI can include a housing, and a container positioned in the housing. The container can contain a liquid and at least a portion of the container can be frangible. The BI can further include a first chamber and a second chamber. The second chamber can include at least one source of biological activity. The BI can further include a first fluid path positioned to fluidly couple the first chamber and the second chamber, and a second fluid path positioned to allow displaced gas to move out of the second chamber. The method can include moving displaced gas out of the second chamber via the second fluid path as a sterilant is moved into the second chamber via the first fluid path and/or as the liquid is moved into the second chamber via the first fluid path. | 2013-11-14 |
20130302850 | METHOD AND EQUIPMENT FOR THE AUTOMATED TESTING OF MICROBIOLOGICAL SAMPLES - By using the method and equipment according to this invention it is possible to analyse rapidly a large number of microbiological culture samples, or alternatively of liquid phase samples, based on the gases or gaseous compounds released by them. This invention exploits generally a sample line, along which the samples move, and it can be used for the microbial control tasks in hospitals, industry, hygiene and environmental fields. In this system gas is led into the culture vessels during the growth of the microbe, and gases released by the culture or liquids derived from them are collected into the chambers, capsules or equivalents. | 2013-11-14 |
20130302851 | SYSTEM AND APPARATUS FOR ANALYSIS OF A GUAYULE PLANT IN SITU - A system and method for in-field near infrared spectroscopy (NIRS) analysis of rubber and resin concentrations a guayule plant is provided. The system includes a wagon or other vehicle with the NIRS device mounted on the wagon. A computer or processor electrically coupled to the NIRS device is also housed within an area or extension of the wagon. During measurement of a guayule plant in the field, a guayule plant covering is placed over the guayule plant and a light shield coupled to the NIRS device is inserted into an opening on the guayule plant covering. The NIRS device is configured to perform a reading of the guayule plant within the plant covering and communicate results of the reading to the computer. A calibration equation is then preferably applied to the guayule plant readings to produce the rubber and resin concentrations of the guayule plant. | 2013-11-14 |
20130302852 | Hematoxylin Staining Method - The present invention relates to processes for staining biological samples, and in particular to automated processes for staining biological sample with hematoxylin stains. In the processes and systems of the invention, separate hematein and mordant solutions are provided which may be premixed prior to application to a biological sample. This method prevents precipitation common in hematein staining solutions and which fouls automated slide/sample processing equipment. | 2013-11-14 |
20130302853 | COMPOSITIONS AND METHODS OF PRODUCING ENTEROKINASE IN YEAST - The present specification disclose polynucleotide molecules encoding an enterokinase, yeast expression constructs including a yeast expression vector and a polynucleotide molecules encoding an enterokinase, yeast cells comprising such a yeast expression construct, methods of producing enterokinase using such yeast cells, and method of cleaving or preparing a recombinant polypeptide using an enterokinase produced by such methods. | 2013-11-14 |
20130302854 | METHOD FOR TREATMENT OF CELLULOSIC BIOMASS AND METHOD FOR PRODUCTION OF SUGAR, ALCOHOL, OR ORGANIC ACID FROM CELLULOSIC BIOMASS - This invention is intended to improve saccharification efficiency by allowing cellulose and/or hemicellulose to relax sufficiently with an ionic liquid. Cellulose and/or hemicellulose is allowed to relax sufficiently with an ionic liquid through a step of soaking cellulosic biomass in a solution containing an ionic liquid and an alkali. | 2013-11-14 |
20130302855 | GENOMICS OF ACTINOPLANES UTAHENSIS - The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type-and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene-and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield. | 2013-11-14 |
20130302856 | METHODS OF DIRECTLY EXTRACTING MICRORNA FROM MICROVESICLE IN CELL LINE, CELL CULTURE, OR BODY FLUID - A method of extracting a nucleic acid from a microvesicle, the method comprising treating the microvesicle with a composition comprising a detergent and an aprotic solvent to extract a nucleic acid from the microvesicle. | 2013-11-14 |
20130302857 | TREHALOSE PHOSPHORYLASES AND THEIR USE IN THE BIOCATALYTIC PRODUCTION OF TREHALOSE-ANALOGUES AND GLYCOSYL PHOSPHATES - The present invention relates to trehalose phosphorylases which are useful for the industrial production of trehalose-analogues and glycosyl phosphates. More specifically, the invention discloses trehalose phosphorylases which are mutated in specific amino acid regions. These specific mutations result in modified substrate specificities of the enzymes. In addition, the present invention discloses a wild type trehalose phosphorylase from the marine organism | 2013-11-14 |
20130302858 | METHODS OF COMBINED BIOPROCESSING AND RELATED MICROORGANISMS, THERMOPHILIC AND/OR ACIDOPHILIC ENZYMES, AND NUCLEIC ACIDS ENCODING SAID ENZYMES - A genetically modified organism comprising: at least one nucleic acid sequence and/or at least one recombinant nucleic acid isolated from | 2013-11-14 |
20130302859 | Engineered imine reductases and methods for the reductive animation of ketone and amine compounds. - The present disclosure provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds. | 2013-11-14 |
20130302860 | Coryneform Bacterium Transformant and Process for Producing Aniline Using The Same - Provided is an aniline-producing transformant constructed by introducing a gene which encodes an enzyme having aminobenzoate decarboxylase activity into a coryneform bacterium as a host. Also provided is a process for producing aniline, which comprises a step of allowing the transformant to react in a reaction mixture containing aminobenzoic acid, an ester thereof, and/or a salt thereof under reducing conditions, and a step of recovering aniline from the reaction mixture. | 2013-11-14 |
20130302861 | EXPRESSION CONSTRUCTS AND USES THEREOF IN THE PRODUCTION OF TERPENOIDS IN YEAST - A method of producing at least one terpene in a yeast cell is disclosed. The method comprises exogenously expressing within the mitochondria of the yeast cell or directing localization thereto a terpene synthase. | 2013-11-14 |
20130302862 | FERMENTATION METHOD FOR PRODUCING CO-ENZYME Q10 - A fermentation method for producing coenzyme Q10 is provided, including stepwisly culturing of microbial strains capable of producing coenzyme Q10, wherein key promoting factors are added in each stage of culture, and in the stage of culture in a fermentor, dissolved oxygen feedback-fed batch culture technique is adopted to realize the feedback regulation of the production of coenzyme Q10, so as to improve the yield of coenzyme Q10. | 2013-11-14 |
20130302863 | COMPOSITIONS CONTAINING HIGH OMEGA-3 AND LOW SATURATED FATTY ACID LEVELS - Food products and supplements comprising the essential unsaturated fatty acids EPA or DHA at high concentrations relative to other n-3 HUFAs and with relatively low levels of saturated fatty acids (myristic and palmitic acids) are provided. These compositions can be made in commercial quantities in a cost effective manner by culture of selected microorganisms. The food products and supplements are suited to human health needs which are unable to be met from fish oil origins. Also provided are EPA containing food products obtained from animals. | 2013-11-14 |
20130302864 | Method for Producing a Fatty Acid Ester - A method for producing a fatty acid ester, which comprises: (a) incubating a culture of microalga at a mid-temperature, wherein the culture is obtained by culturing the microalga in a medium, (b) then adding an alcohol, allowing a reaction at a temperature lower than the mid-temperature, and (c) collecting a fatty acid ester from an obtained reaction product. | 2013-11-14 |
20130302865 | Polypeptides Having C4 Dicarboxylic Acid Transporter Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having C4-dicarboxylic acid transporter activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, and methods of producing C4-dicarboxylic acids, such as malic acid. | 2013-11-14 |
20130302866 | COMPOSITIONS AND METHODS FOR SUCCINATE PRODUCTION - The present application provides genetically modified yeast cell comprising an active succinate fermentation pathway, as well as methods of using these cells to produce succinate. | 2013-11-14 |
20130302867 | METHOD FOR PRODUCING BIODEGRADABLE POLYMER AND BIOMASS FUEL CONVERTED FROM CARBON SOURCE BY RECOMBINANT MICROORGANISMS - Disclosed is a method for producing biodegradable polymer and biomass fuel converted from carbon source by using recombinant microorganisms, comprising the steps of: (A) providing recombinant microorganisms transformed with plasmids containing at least a gene encoding for glycerol utilizing enzyme and a gene encoding for polyhydroxyalkanoate synthase; (B) culturing the recombinant microorganisms in a medium containing glycerol; (C) inducing expression of the genes of step (A), thereby obtaining polyhydroxyalkanoate and ethanol; and (D) recovering the polyhydroxyalkanoate and the ethanol; wherein the recombinant microorganisms have a glycerol utilization rate more than 90% (w/w), and have polyhydroxyalkanoate accumulated therein to a biomass content thereof at least 30% (w/w). | 2013-11-14 |
20130302868 | Isobutanol Production Using Yeasts With Modified Transporter Expression - The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise one or more modifications resulting in the reduction in the expression and/or activity of an endogenous transporter protein. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 2013-11-14 |
20130302869 | OPTOFLUIDIC PHOTOBIOREACTOR APPARATUS, METHOD, AND APPLICATIONS - An optofluidic photobioreactor including an optical waveguide having an input, characterized by an evanescent optical field confined along an outer surface of the optical waveguide produced by radiation propagating in the optical waveguide, means for inputting light to the input of the optical waveguide, and a selected photosynthetic microorganism disposed substantially within the evanescent field. A method for optically exciting a photosynthetic microorganism for generating a biofuel, a biofuel precursor, or a biomass from the optically-excited photosynthetic microorganism involves irradiating the photosynthetic microorganism attached to the surface of the waveguide with an evanescent optical field from optical radiation propagating in the optical waveguide, and driving photosynthesis in the microorganism by the evanescent optical field. | 2013-11-14 |
20130302870 | Flowthrough Pretreatment Of Lignocellulosic Biomass And Selective Separation Of Components Using High-Temperature Nanoporous Membranes - A new and improved biomass conversion system is disclosed using high-temperature flow-though pretreatment and a nanoporous membrane to provide more digestible biomass for subsequent conversion to biofuels. | 2013-11-14 |
20130302871 | METHOD AND DEVICE FOR OPERATING A FERMENTATION PLANT - A method for operating a fermentation plant with at least one digester that is supplied with a substrate. The substrate is percolated using a percolate from a percolate container and biogas that is generated via the percolation process in the digester or/and in the percolate container is drawn off. The drawn off biogas is treated in a biogas treatment device and the CO | 2013-11-14 |
20130302872 | PRODUCTION OF CELL TISSUE HAVING THREE-DIMENSIONAL STRUCTURE USING ELECTROSTATIC INK JET PHENOMENON - A method and an apparatus for producing a cell tissue of blood vessels or the like with the use of an electrostatic ink jet phenomenon are provided. A pattern generator for cell tissue patterning on a substrate includes at least one vessel for holding a solution containing materials used for cell tissue formation, a substrate that is an object of patterning, and a pattern generator including a voltage applying unit for applying a voltage to the vessel. The voltage is applied to the vessel by the voltage applying unit to generate an electric field between the vessel and the substrate, causing the solution to be ejected from the vessel as a result of an electrostatic ink jet phenomenon and allowing the ejected solution to adhere to the substrate. | 2013-11-14 |
20130302873 | Methods of Making Material Coatings for Self-cleaning and Self-decontamination of Metal Surfaces - A method of making a composite structure exhibiting the ability to degrade chemical or biological agents upon contact comprising a substrate to be protected from the deleterious effects of chemical or biological agents possessing surface groups capable of deactivating materials having the ability to degrade chemical or biological agents, a buffer film, coated onto the substrate, that blocks the ability of the substrate surface groups to deactivate the materials having the ability to degrade chemical or biological agents, and a protective film, coated onto the buffer film, containing materials having the ability to degrade chemical or biological agents encapsulated in or comprising the outer surface of the protective film. | 2013-11-14 |
20130302874 | ENZYME PREPARATIONS - The invention is directed to enzyme preparations which are obtainable by providing enzyme immobilizates which comprise enzymes or microorganisms comprising enzymes immobilized on an inert support with a polyethersilicone coating obtained by hydrosilylation, to a process for preparing such enzyme preparations and to the use of enzyme preparations as an industrial biocatalyst. | 2013-11-14 |
20130302875 | NOVEL USE OF LIPOLYTIC ENZYME FOR FORMATION OF ANTI-FINGERPRINT COATING, METHOD OF FORMING ANTI-FINGERPRINT COATING, SUBSTRATE COMPRISING THE ANTI-FINGERPRINT COATING FORMED BY THE METHOD, AND PRODUCT COMPRISING THE SUBSTRATE - Provided are a novel use of a lipolytic enzyme for forming anti-fingerprint coating, a method of forming anti-fingerprint coating including treating a substrate with a composition comprising the lipolytic enzyme, a substrate including the anti-fingerprint coating formed by the same method, and a product including the same. The anti-fingerprint coating can reduce contamination of display devices, appearances of electronic devices or building materials by fingerpris. | 2013-11-14 |
20130302876 | Transducible Polypeptides For Modifying Metabolism - Methods and compositions for modifying the metabolism of a subject are provided. One embodiment provides a recombinant polypeptide having a polynucleotide-binding domain, a protein transduction domain, and a targeting domain. In a preferred embodiment, the polynucleotide-binding domain includes one or more HMG box domains. | 2013-11-14 |
20130302877 | Differential Functionalization of Polymers with Amino-Oxy Reagents for Diagnostic Assays - This invention relates to a method of conveniently functionalizing the reducing end of a carbohydrate polymer. The method also allows for the length of the polymer to be differentially functionalized. The invention provides a method for preparing polymers with a different functional groups or functionality on the sides than on the reducing end. An advantage of the method of the invention is that the procedures are simpler, preferably requiring less time, fewer steps, reduced temperatures and less harsh chemical components than conventional procedures for differential modification, with the further advantage that the reactions can be carried out in aqueous media. | 2013-11-14 |
20130302878 | EXPRESSION OF PLANT PEROXIDASES IN FILAMENTOUS FUNGI - The present invention relates to recombinant expression of plant derived peroxidases in filamentous fungal host organisms. | 2013-11-14 |
20130302879 | Processes for Treating Textile with Polypeptide Having Cellulolytic Enzyme Enhancing Activity - The present invention relates to the use of glycosyl hydrolase family (61) polypeptides in the presence of cellulases for textile manufacture as well as a textile composition comprising glycosyl hydrolase family (61) polypeptides and cellulases. | 2013-11-14 |
20130302880 | POLYNUCLEOTIDES ENCODING MUTANT HYDROLASE PROTEINS WITH ENHANCED KINETICS AND FUNCTIONAL EXPRESSION - The invention provides a mutant hydrolase protein with enhanced kinetics and functional expression, as well as polynucleotides encoding the mutant proteins and methods of using the polynucleotides and mutant proteins. | 2013-11-14 |
20130302881 | METHOD FOR PRODUCING REFINED VEGETABLE OIL - A method of producing a refined, bleached and deodorised vegetable oil comprises degumming a vegetable oil, bleaching the degummed oil and deodorising the bleached oil, wherein the degumming, bleaching and deodorising conditions are selected to reduce the content of 3-chloropropane-1,2-diol and esters thereof in the deodorised oil to less than 8 ppm. | 2013-11-14 |
20130302882 | METHOD FOR STERILIZING SEPARATION MEMBRANE MODULES, STERILIZATION DEVICE, AND APPARATUS FOR PRODUCING CHEMICALS - A method of sterilizing a separation membrane module reduces damage of a ceramic separation membrane, a sterilization process device achieves the method of sterilizing, and an apparatus produces chemicals. The method of sterilizing the separation membrane module includes a ceramic-containing separation membrane including a temperature raise step of raising the temperature of the separation membrane module to a predetermined sterilization temperature by feeding sterilization water having a controlled temperature and pressure so that a rate of temperature change of the separation membrane module is 6.0° C. or less per minute, and a sterilization step of sterilizing the separation membrane module at a predetermined temperature for a predetermined time. | 2013-11-14 |
20130302883 | METHODS, SYSTEMS AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS - Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases. | 2013-11-14 |
20130302884 | METHODS, SYSTEMS AND DEVICES FOR MULTIPLE SINGLE-CELL CAPTURING AND PROCESSING USING MICROFLUIDICS - Methods, systems, and devices are described for multiple single-cell capturing and processing utilizing microfluidics. Tools and techniques are provided for capturing, partitioning, and/or manipulating individual cells from a larger population of cells along with generating genetic information and/or reactions related to each individual cell. Different capture configurations may be utilized to capture individual cells and then processing each individual cell in a multi-chamber reaction configuration. Some embodiments may provide for specific target amplification, whole genome amplification, whole transcriptome amplification, real-time PCR preparation, copy number variation, preamplification, mRNA sequencing, and/or haplotyping of the multiple individual cells that have been partitioned from the larger population of cells. Some embodiments may provide for other applications. Some embodiments may be configured for imaging the individual cells or associated reaction products as part of the processing. Reaction products may be harvested and/or further analyzed in some cases. | 2013-11-14 |
20130302885 | Method of Forming Cell Spheroids Cultured in Serum-Free Manner on Nanoscale Coatings of Hyaluronic Acid with High Molecular Weight - A method of forming cell spheroids cultured in a serum-free manner on nanoscale coatings of hyaluronic acid with high molecular weight is disclosed. Corneal stromal cells are cultured on the hyaluronic acid coatings with nanotopography in the serum-free manner. Experimental results show that the cells cultured on the hyaluronic acid coatings (1.1-1.7 nm) increase cell-cell interaction, and when the cells form three-dimensional spheroids, they have higher biological synthetic capabilities and can secret more extracellular matrix, which has potential applications in corneal stromal tissue reconstruction. | 2013-11-14 |
20130302886 | Pre-Ligand assembly domain of the IL-17 receptor - The invention provides isolated Pre-Ligand Assembly Domain (PLAD) polypeptides which inhibit multimerization of a receptor complex comprising an IL-17R family member. Related PLAD-binding polypeptides, chimeric proteins, conjugates, nucleic acids, vectors, host cells, and methods are provided herein. | 2013-11-14 |
20130302887 | COMPOSITIONS AND METHODS FOR GROWING HUMAN EMBRYONIC CELLS - Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing secreted products obtained from the culture medium of human embryonic gem (EG) cell derivatives, such as embryoid body-derived cells. Substrates include compounds such as collagen I, fibronectin, or superfibronectin, or extracellular matrix, typically human derived. | 2013-11-14 |
20130302888 | CELL CULTURE METHOD AND SYSTEM FOR ESTABLISHING AN IN VITRO MODEL OF THE INTESTINAL BARRIER - Cell culture process includes seeding a suitable culture medium with enterocytes and then, after a delay, seeding the medium containing the enterocytes that have begun to proliferate, with goblet cells. | 2013-11-14 |
20130302889 | Differentiation of Bone Marrow Cells into Neuronal Cells and Uses Therefor - The present invention relates to methods of inducing differentiation of mammalian bone marrow stromal cells into neuronal cells by contacting marrow stromal cells with a neuronal differentiation-inducing compounds. Neuronal differentiation-inducing compounds of the invention include anti-oxidants such as, but not limited to, beta-mercaptoethanol, dimethylsulfoxide, butylated hydroxyanisole, butylated hydroxytoluene, ascorbic acid, dimethylfumarate, and n-acetylcysteine. Once induced to differentiate into neuronal cells, the cells can be used for cell therapy, gene therapy, or both, for treatment of diseases, disorders, or conditions of the central nervous system. | 2013-11-14 |
20130302890 | STEM CELLS & MATRIX FROM CORD TISSUE - Methods for isolating and culturing stem cells, making tissue matrix, making matrix infused with stem cells, and methods of stem cell therapy are provided. | 2013-11-14 |
20130302891 | COMPOSITIONS AND METHODS FOR PROMOTING LIPOSOMAL AND CELLULAR ADHESION - The present application describes compounds, compositions and methods for incorporating chemoselective and bio-orthogonal complementary functional groups into liposomes. The present application also describes various uses of these modified liposomes including for tethering the chemoselective and bio-orthogonal complementary functional groups from cell surfaces by liposome delivery toward the goal of rewiring the cell surface. | 2013-11-14 |
20130302892 | THERAPEUTIC APPLICATIONS OF P53 ISOFORMS IN REGENERATIVE MEDICINE, AGING AND CANCER - The present invention provides methods and compositions for modulating cell senescence and cell proliferation using isoforms of the p53 tumor suppressor protein. The methods and compositions of the invention find use in inhibiting cancer cell growth or in generating populations of cells for tissue regeneration through the modulation of cell senescence and proliferation. | 2013-11-14 |
20130302893 | METHOD FOR INCREASING THE EFFICIENCY OF INDUCING PLURIPOTENT STEM CELLS - The present invention relates to a method for increasing the efficiency of inducing pluripotent stem cells, and more particularly, to a method for increasing the efficiency of inducing pluripotent stem cells by utilizing genes Jhdm1b and Jhdm1a that modify histone. By utilizing Jhdm1b, Jhdm1a, and a stem cell inducing factor, the present invention increases the efficiency of inducing pluripotent stem cells and increases the quality of induced pluripotent stem cells. The stem cell inducing factor is a combination of Oct4 and Klf4, or a combination of Sox2, Oct4, and Klf4, or a combination of Oct4 and Sox2, and Oct4 alone. The method of the present invention further comprises exposing the cells to vitamin C, which further increases the efficiency of inducing pluripotent stem cells as compared with the case where no vitamin C is used. By using less stem cell reducing factors, the method of the present invention reduces the potential carcinogenicity, obtains a high inducing efficiency, and provides high-quality induced pluripotent stem cells capable of germ-line transmission. | 2013-11-14 |
20130302894 | Polymeric Film for Use in Bioprocessing Applications - The presently disclosed subject matter is directed to a polymeric films suitable for use in a wide variety of applications, including (but not limited to) the formation of bioprocessing containers. Particularly, the disclosed films comprise a first barrier layer comprising polyglycolic acid, polyamide, EVOH, and/or an EVOH blend and a second barrier layer comprising EVOH. The dual barrier layers of the film maintain a high gas barrier under a variety of relative humidity conditions. In addition, the disclosed film is biologically inert and free from film surface-modifying additives such that the film does not inhibit the growth of biological cell cultures. | 2013-11-14 |
20130302895 | CORAL-DERIVED COLLAGEN AND METHODS OF FARMING SAME - An isolated collagen fiber is disclosed, wherein a length of the fiber prior to stretching by about 15%, is identical to a length of the fiber following said stretching by about 15%. The fiber comprises a Nuclear Magnetic Resonance (NMR) spectroscopic profile as shown in FIG. | 2013-11-14 |
20130302896 | SOY-PROTEIN CONTAINING POROUS MATERIALS - Porous soy protein-based materials are provided. Also provided are tissue growth scaffolds comprising the porous soy protein-based materials. Methods for forming the porous soy protein-based materials and methods for growing tissue on the tissue growth scaffolds are also provided. The porous soy protein-based materials comprise a plurality of soy protein chains that are crosslinked in a three-dimensional structure that provides a high degree of porosity. In order to achieve highly porous structures, the soy proteins, which are globular in nature, can be partially denatured in order to facilitate crosslinking between, and entanglement of, the soy protein chains. | 2013-11-14 |
20130302897 | DEFORMABLE TRANSPORTABLE BIOREACTOR CHAMBER - An apparatus and method is described for seeding and culturing cells on a sample. The apparatus includes a chamber in which the volume of the chamber may be adjusted without compromising the seal or sterility of the chamber. The apparatus enables the seeding of cells in a reduced volume and culturing of cells in an increased volume. Further, the apparatus enables application of forces, strains and torques to a sample during seeding, culturing or transportation of the sample. | 2013-11-14 |
20130302898 | METHODS OF IMPROVING TITER IN TRANSFECTION-BASED PRODUCTION SYSTEMS USING EUKARYOTIC CELLS - The invention relates to methods of improving titer in transfection-based bioreactor culture production or transfection-based production systems using eukaryotic cells. | 2013-11-14 |
20130302899 | MULTIFUNCTIONAL ALLELES - Nucleic acid constructs and methods for rendering modifications to a genome are provided, wherein the modifications comprise null alleles, conditional alleles and null alleles comprising COINs. Multifunctional alleles (MFA) are provided, as well as methods for making them, which afford the ability in a single targeting to introduce an allele that can be used to generate a null allele, a conditional allele, or an allele that is a null allele and that further includes a COIN. MFAs comprise pairs of cognate recombinase recognition sites, an actuating sequence and/or a drug selection cassette, and a nucleotide sequence of interest, and a COIN, wherein upon action of a recombinase a conditional allele with a COIN is formed. In a further embodiment, action of a second recombinase forms an allele that contains only a COIN in sense orientation. In a further embodiment, action by a third recombinase forms an allele that contains only the actuating sequence in sense orientation. | 2013-11-14 |
20130302900 | CRYSTALLIZED DIACETYLENIC INDICATOR COMPOUNDS AND METHODS OF PREPARING THE COMPOUNDS - Crystallized diacetylenic compounds having certain crystallographic and other characteristics; diacetylenic compounds and mixtures crystallized from diacetylenic solutions; methods of preparing and identifying solvent systems for dissolving diacetylenic compounds; diacetylenic solutions; methods of recrystallizing diacetylenic compounds; crystals of 2,4-hexadiyn-1,6-bis(alkylurea) compounds; and ambient condition indicators and time-temperature condition indicators comprising crystallized diacetylenic compounds. | 2013-11-14 |
20130302901 | Electrodes for Sensing Chemical Composition - Some embodiments of the present disclosure provide methods, devices, and systems for sequencing nucleic acid polymers that utilize palladium (Pd), for example, at least in part, as an electrode material that is (i) functionalized with one or more adaptor molecules and (ii) capable for use to sense one or more chemical compositions. | 2013-11-14 |
20130302902 | NANOFIBRIL MATERIALS FOR HIGHLY SENSITIVE AND SELECTIVE SENSING OF AMINES - A sensory material with high sensitivity, selectivity, and photostability has been developed for vapor probing of organic amines. The sensory material is a perylene-3,4,9,10-tetracarboxyl compound having amine binding groups and the following formula | 2013-11-14 |
20130302903 | FLUORESCENT NITRIC OXIDE PROBES AND ASSOCIATED METHODS - Nitric oxide probes comprising a compound represented by Formula, I, II, III, IV, V, VI or a combination thereof are provided. Methods of using these nitric oxide probes to detect nitric oxide are also provided. | 2013-11-14 |
20130302904 | Chemosensors for Hydrogen Sulfide - Selective chemosensing agents for hydrogen sulfide are provided. The chemosensing agents can act fast under mild conditions, are chemically stable for long-term storage, are sensitive for detection under near physiological conditions, show a linear concentration-signal relationship within physiologically relevant hydrogen sulfide concentration ranges for easy quantitation, show minimal or no interference by other anions in the blood, and are functional in aqueous solutions and blood plasma. | 2013-11-14 |