45th week of 2019 patent applcation highlights part 27 |
Patent application number | Title | Published |
20190338255 | BIOSYNTHESIS OF BENZYLISOQUINOLINE ALKALOIDS AND BENZYLISOQUINOLINE ALKALOID PRECURSORS - Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to have reduced levels or activity of one or more alcohol dehydrogenases or aldehyde reductases thereby increasing the production of benzylisoquinoline alkaloids and/or benzylisoquinoline alkaloid precursors. | 2019-11-07 |
20190338256 | REDOX BALANCING IN YEAST - Described are composition and methods relate to reducing the redox imbalance in anaerobically growing yeast with attenuated glycerol production by re-engineering the pathway for Ac-CoA biosynthesis. | 2019-11-07 |
20190338257 | ELECTRON TRANSPORT CHAIN MODULE FROM EUKARYOTIC ORGANELLE AND APPLICATION THEREOF - Provided are an electron transport chain module from a eukaryotic organelle and an application thereof in biological nitrogen fixation. The electron transport chain (ETC) module is composed of the NifJ protein from | 2019-11-07 |
20190338258 | NUCLEIC ACID AMPLIFICATION - The present disclosure provides methods, compositions, kits and systems for nucleic acid amplification. In some embodiments, nucleic acid amplification methods include subjecting the nucleic acid to be amplified to partially denaturing conditions. In some embodiments, nucleic acid amplification methods include amplifying without fully denaturing the nucleic acid that is amplified. In some embodiments, the nucleic acid amplification method employs an enzyme that catalyzes homologous recombination and a polymerase. In some embodiments, methods for nucleic acid amplification can be conducted in a single reaction vessel and/or in a single continuous liquid phase of a reaction mixture, without need for compartmentalization of the reaction mixture or immobilization of reaction components. In some embodiments, methods for nucleic acid amplification comprise amplifying at least one polynucleotide onto a surface under isothermal amplification conditions, optionally in the presence of a polymer which can include a sieving agent and/or a diffusion-reducing agent. | 2019-11-07 |
20190338259 | ENGINEERED BIOSYNTHETIC PATHWAYS FOR PRODUCTION OF (6E)-8-HYDROXYGERANIOL BY FERMENTATION - The present disclosure describes the engineering of microbial cells for fermentative production of (6E)-8-hydroxygeraniol and provides novel engineered microbial cells and cultures, as well as related (6E)-8-hydroxygeraniol production method. | 2019-11-07 |
20190338260 | Lipase Variants and Polynucleotides Encoding Same - The present invention relates to a lipase variant of a parent lipase, which variant has lipase activity, at least 75% but less than 100% sequence identity to SEQ ID NO: 3 and comprises a substitution at one or more positions corresponding to positions 1; 2; 3; 4; 5; 6; 7; 9; 10; 11; 12; 16; 19; 30; 31; 34; 36; 37; 39; 40; 42; 44; 51; 52; 53; 54; 56; 58; 59; 70; 71; 72; 73; 83; 84; 86; 88; 90; 92; 93; 95; 96; 100; 101; 102; 104; 106; 109; 110; 112; 117; 119; 124; 125; 127; 128; 131; 132; 133; 134; 135; 137; 158; 159; 160; 161; 162; 163; 165; 166; 167; 168; 170; 181; 182; 183; 189; 190; 192; 194; 196; 202; 210; 211; 212; 220; 225; 227; 228; 229; 230; 231; 233; 237; 238; 239; 240; 242; 246; 247; 248; 252; 259; 262; 264; 269 of SEQ ID NO: 3. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2019-11-07 |
20190338261 | LIPOLYTIC ENZYME VARIANTS - The present disclosure describes a variant polypeptide having lipolytic activity, wherein the variant has an amino acid sequence which, when aligned with the amino acid sequence as set out in SEQ ID NO: 2, comprises at least one substitution of an amino acid residue at a position corresponding to any of the positions 113, 122, 138, 141, 179, 282, 284, 286, 295, said positions being defined with reference to SEQ ID NO: 2, and wherein said variant has at least 70% identity with the mature polypeptide having lipolytic activity as set out in SEQ ID NO: 2. | 2019-11-07 |
20190338262 | MODULAR EXTRACELLULAR SENSOR ARCHITECTURE FOR REGULATING GENES - Disclosed are systems, components of systems, and methods for sensing extracellular ligands and/or modulating expression of an endogenous or exogenous gene in a cell. In some embodiments, the disclosed systems and methods comprise or utilize first and second exogenous extracellular sensors and/or nucleic acid sequences encoding the first and second exogenous extracellular sensors, wherein the first exogenous extracellular sensor comprises: a) a ligand binding domain; b) a transmembrane domain; c) a protease cleavage site; and d) a functional domain comprising an RNA-binding subdomain fused to a transcription regulatory subdomain; and the second exogenous extracellular sensor comprises: e) a ligand binding domain, f) a transmembrane domain, and g) a protease domain that cleaves the protease cleavage site of the first exogenous extracellular receptor. | 2019-11-07 |
20190338263 | OPTIMIZED ENGINEERED MEGANUCLEASES HAVING SPECIFICITY FOR A RECOGNITION SEQUENCE IN THE HEPATITIS B VIRUS GENOME - The present invention encompasses engineered nucleases which recognize and cleave a recognition sequence within a Hepatitis B virus (HBV) genome. The engineered meganucleases can exhibit at least one optimized characteristic, such as enhanced specificity and/or efficiency of indel formation, when compared to the first-generation meganuclease HBV 11-12x.26. Further, the invention encompasses pharmaceutical compositions comprising engineered meganuclease proteins, nucleic acids encoding engineered meganucleases, and the use of such compositions for treating HBV infections or hepatocellular carcinoma. | 2019-11-07 |
20190338264 | VARIANT AMYLASE ENZYME COMPOSITIONS AND METHODS - The invention is directed to novel amylases. | 2019-11-07 |
20190338265 | METHOD OF PRODUCING MONOMERIC CARD IN BACTERIA - Provided is a method of producing monomeric caspase activation and recruitment domains (CARDs) in bacteria. According to this method, CARD domains may be easily produced in a monomeric form. Monomeric CARDs may be applied to studies on the mechanism of action of caspase, the development of new drugs, detection and elimination of endotoxins, development of cosmetics, etc. | 2019-11-07 |
20190338266 | Subtilase Variants and Polynucleotides Encoding Same - The present invention relates to novel subtilase variants exhibiting increased stability and optionally on par or improved wash performance. The variants of the invention are suitable for use in e.g. cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention. | 2019-11-07 |
20190338267 | PROTEASE VARIANTS ACTIVE OVER A BROAD TEMPERATURE RANGE - The present invention provides protease compositions particularly suited for dishwashing applications. | 2019-11-07 |
20190338268 | Complement Factor B Analogs and Their Uses - The invention provides polypeptides comprising a complement factor B analog. The invention also provides various complement factor B analogs including complement factor B analogs comprising a mutation of a free cysteine amino acid and related methods, nucleic acids and vectors. These complement factor B analogs and related methods, nucleic acids and vectors can be used to modulate a complement pathway or for the study and/or treatment of various conditions or diseases related to a complement pathway. | 2019-11-07 |
20190338269 | FACTOR X VARIANTS - Disclosed is a protein that is a factor X variant including a mutated sequence of SEQ ID NO:1; at its N-terminus, the protein includes the signal peptide of sequence SEQ ID NO:7 and a propeptide that differs from the natural factor X propeptide. | 2019-11-07 |
20190338270 | IMMUNOGENIC ARGINASE PEPTIDES - The present invention relates to immunogenic polypeptide fragments of a human Arginase protein. The fragments are in particular useful for the treatment or prevention of cancer. | 2019-11-07 |
20190338271 | IMMUNOPROTECTION OF PANCREATIC ISLETS - A method for producing immunoprotected pancreatic islets including forming double-layer PEGylated pancreatic islets by adding a first heterobifunctional polyethylene glycol (PEG) molecule and a second heterobifunctional PEG molecule to pancreatic islets and forming immunoprotected pancreatic islets by conjugating a plurality of JAG-1 peptides to the double-layer PEGylated pancreatic islets. | 2019-11-07 |
20190338272 | MASSIVELY MULTIPLEXED HOMOLOGOUS TEMPLATE REPAIR FOR WHOLE-GENOME REPLACEMENT - Disclosed are systems and methods for whole-genome replacement through a massively multiplexed homologous template repair process. Disclosed aspects include a method of substantially changing a DNA sequence of an organism, the method including one or more of the following steps: determining a desired DNA sequence, the desired DNA sequence being a DNA sequence to which it is desired that the DNA sequence of the organism be substantially changed; preparing a treatment configured to cause the organism DNA sequence to be substantially changed to the desired DNA sequence; applying the treatment to the organism; wherein the treatment is configured to cause the organism DNA sequence to be substantially changed to the desired DNA sequence by causing, at each of multiple sites in the organism DNA sequence, genetic code at the site to be substantially changed to genetic code at a responding site in the desired DNA sequence; each of the multiple sites in the organism DNA sequence is a respective sub-sequence of the organism DNA sequence; applying the treatment includes delivering to the organism at least one dose; and each dose includes respective change agent material that causes the changing of the genetic code at a respective plurality of the multiple sites. In certain embodiments, the DNA sequence of the organism is a whole-genome DNA sequence of the organism, and the desired DNA sequence is substantially the organism's germline whole-genome DNA sequence, an intentionally modified version of the organism's germline whole-genome DNA sequence, or a whole-genome DNA sequence of another organism. | 2019-11-07 |
20190338273 | METHOD TO OVERCOME DNA CHEMICAL MODIFICATIONS SENSITIVITY OF ENGINEERED TALE DNA BINDING DOMAINS - The present invention relates to polypeptides and more particularly to Transcription Activator-Like Effector derived proteins that allow to efficiently target and/or process nucleic acids. Particularly, the present invention reports the characterization of TALE derived proteins that can efficiently target methylated DNA. The present invention more specifically relates to TALE derived proteins that allow activation of methylated promoters responsible for gene silencing. | 2019-11-07 |
20190338274 | Methods, Cells & Organisms - The invention relates to an approach for introducing one or more desired insertions and/or deletions of known sizes into one or more predefined locations in a nucleic acid (eg, in a cell or organism genome). They developed techniques to do this either in a sequential fashion or by inserting a discrete DNA fragment of defined size into the genome precisely in a predefined location or carrying out a discrete deletion of a defined size at a precise location. The technique is based on the observation that DNA single-stranded breaks are preferentially repaired through the HDR pathway, and this reduces the chances of indels (eg, produced by NHEJ) in the present invention and thus is more efficient than prior art techniques. The invention also provides sequential insertion and/or deletions using single- or double-stranded DNA cutting. | 2019-11-07 |
20190338275 | METHODS AND KITS - A method of detection and identification of one or more microorganism/s in a biological sample comprising the following steps:
| 2019-11-07 |
20190338276 | METHODS FOR THE SELECTION OF BINDING PROTEINS - This application provides an improved screening method for the selection of target-binding proteins having desirable biophysical properties. The method combines mRNA display and yeast surface display in a way that takes advantage of the desirable attributes of both processes. | 2019-11-07 |
20190338277 | RNA-YY1 Interactions - Methods relating to obtaining libraries of YY1-binding long non-coding RNAs, libraries obtained thereby, and methods of use thereof. | 2019-11-07 |
20190338278 | MOLECULAR BARCODING ON OPPOSITE TRANSCRIPT ENDS - Disclosed herein include systems, methods, compositions, and kits for molecular barcoding on the 5′-end of a nucleic acid target. After barcoding a nucleic acid target using an oligonucleotide barcode comprising a target binding region and a molecular label to generate a barcoded nucleic acid molecule, an oligonucleotide comprising a complement of the target binding region can be added to generate a barcoded nucleic acid molecule comprising the target-binding region and the complement of the target-binding region. A stem loop is formed with intra-molecular hybridization of the barcoded nucleic acid molecule, which can be extended to generate an extended barcoded nucleic acid molecule comprising the molecular label and a complement of the molecular label. | 2019-11-07 |
20190338279 | METHOD FOR CONSTRUCTING SINGLE CELL SEQUENCING LIBRARY AND USE THEREOF - Provided in the present invention is a method for constructing a single cell sequencing library, comprising the following steps: a) lysing a single cell to obtain a single cell lysate; b) separating the nucleus and the cytoplasm in the single cell lysate obtained in step a) to obtain a nuclear solution and a total RNA solution; and c) constructing a chromatin DNA library with the nuclear solution obtained in step b) to obtain a chromatin-accessibility sequencing library of the single cell; and constructing a transcriptome library with the total RNA solution obtained in step b) to obtain a transcriptome sequencing library of the single cell. | 2019-11-07 |
20190338280 | IMMUNOMODULATION BY CONTROLLING ELR+ PROINFLAMMATORY CHEMOKINE LEVELS WITH THE LONG NON-CODING RNA UMLILO - The present invention relates to methods for modulating the production of ELR+ proinflammatory chemokines in a subject or a cell using either UMLILO IncRNA inhibitors to decrease production of ELR+ proinflammatory cytokines or using UMLILO IncRNA's to increase the production of ELR+ proinflammatory cytokines. The invention also provides for the use of UMLILO IncRNA inhibitors or UMLILO IncRNA's to modulate the expression of ERL+ proinflammatory cytokines. | 2019-11-07 |
20190338281 | SELECTIVE ANTISENSE COMPOUNDS AND USES THEREOF - The present invention provides oligomeric compounds. Certain such oligomeric compounds are useful for hybridizing to a complementary nucleic acid, including but not limited, to nucleic acids in a cell. In certain embodiments, hybridization results in modulation of the amount activity or expression of the target nucleic acid in a cell. | 2019-11-07 |
20190338282 | METHOD FOR MODIFYING AMINO ACID ATTENUATOR AND USE OF SAME IN PRODUCTION - The present invention discloses a method for modifying an amino acid attenuator, a class of amino acid attenuator mutants, engineered bacteria created on the basis of the amino acid attenuator mutants, and use of the engineered bacteria. The present invention protects a method for relieving the attenuation regulation of an amino acid operon gene, which is modification of the amino acid operon gene by: removing a gene coding for a leader peptide and an anterior reverse complementary palindromic sequence in the terminator stem-loop structure, and maintaining a posterior reverse complementary palindromic sequence in the terminator. The amino acid operon particularly can be histidine operon, tryptophan operon, phenylalanine operon, alanine operon, threonine operon and etc. The present invention can be used for the production of amino acids and derivatives thereof in fermentation by bacterica, providing a novel method for improving the production of amino acids in fermentation | 2019-11-07 |
20190338283 | METHODS AND COMPOSITIONS FOR TREATING A SUBJECT WITH A SMAD7 ANTISENSE OLIGONUCLEOTIDE - The present invention relates to treatment of inflammatory bowel disease (e.g., Crohn's disease and ulcerative colitis) using antisense nucleotides that are directed against polymorphic forms (e.g., those containing single nucleotide polymorphisms) of the SMAD7 mRNA. The invention thus relates to treatment methods for subjects having polymorphic forms of SMAD7 and antisense oligonucleotides that specifically target SMAD7 mRNA transcripts containing polymorphisms. | 2019-11-07 |
20190338284 | COMPLEX FOR DRUG DELIVERY AND STABILIZATION AND PREPARATION METHOD THEREOF - A drug delivery system includes an atelocollagen-[aptamer-drug] complex which is prepared by mixing an aptamer-drug conjugate comprised of an aptamer and a drug attached to the aptamer with an atelocollagen dispersion. The aptamer is selected from the group consisting of AS1411, CRO, and ERBB2, and the drug is selected from the group consisting of Gemcitabine, Doxorubicin, and siRNA. An anticancer composition including the drug delivery system can compensate for shortcomings of existing anticancer agents and anticancer therapies. | 2019-11-07 |
20190338285 | REAGENTS FOR TREATMENT OF HEPATITIS B VIRUS (HBV) INFECTION AND USE THEREOF - This disclosure relates to RNA interference (RNAi) reagents for treatment of hepatitis B virus (HBV) infection, compositions comprising same, and use thereof to treat individuals infected with HBV. The reagents are artificial miRNA (shmiRNA) used alone or in combination with additional shmiRNA or shRNA. | 2019-11-07 |
20190338286 | ANTISENSE OLIGONUCLEOTIDES FOR MODULATING REL EXPRESSION - The present invention relates to antisense oligonucleotides that are capable of modulating expression of c-Rel in a target cell. The present invention further relates to conjugates of the oligonucleotide and pharmaceutical compositions and methods for treatment of cancer, inflammation or autoimmune diseases using the oligonucleotide. | 2019-11-07 |
20190338287 | RNAi-MEDIATED INHIBITION OF HIF1A FOR TREATMENT OF OCULAR ANGIOGENESIS - RNA interference is provided for inhibition of HIF1A mRNA expression for treating patients with ocular angiogenesis, particularly for treating retinal edema, diabetic retinopathy, sequela associated with retinal ischemia, posterior segment neovascularization (PSNV), and neovascular glaucoma, and for treating patients at risk of developing such conditions. | 2019-11-07 |
20190338288 | MODULATION OF TMPRSS6 EXPRESSION - Disclosed herein are antisense compounds and methods for modulating TMPRSS6 and modulating an iron accumulation disease, disorder and/or condition in an individual in need thereof. Iron accumulation diseases in an individual such as hemochromatosis or β-thalassemia can be ameliorated or prevented with the administration of antisense compounds targeted to TMPRSS6. | 2019-11-07 |
20190338289 | COMPOSITIONS AND METHODS FOR REACTIVATING LATENT IMMUNODEFICIENCY VIRUS - The present disclosure provides compositions and methods for reactivating latent immunodeficiency virus and/or reducing transcription of HIV integrated into the genome of an HIV-infected cell. The present disclosure provides compositions and methods for treating an immunodeficiency virus infection. | 2019-11-07 |
20190338290 | TREATMENT OF SARCOMA - The present invention encompasses the recognition that epigenetic dependencies in sarcoma can be therapeutic targets. The present invention encompasses a method of treating sarcoma comprising the step of administering a PRC1.1 inhibitory agent to a subject suffering from or susceptible to sarcoma. In some embodiments, the PRC1.1 inhibitory agent is a KDM2B inhibitory agent, a BCOR inhibitory agent, and/or a PCGF1 inhibitory agent. In some embodiments, the PRC1.1 inhibitory agent reduces interaction of the SS18-SSX fusion protein with a polycomb repressive complex. In some embodiments, administration of the PRC1.1 inhibitory agent results in differentiation of synovial sarcoma cells into a more mesenchymal like state, comprising increased expression of COL1A1, SERPINE1 (PAI-1), ACTA2 (a-SMA), CDKN1A and/or CDKN2B. | 2019-11-07 |
20190338291 | SNAP25 ANTISENSE OLIGONUCLEOTIDES - Provided are peptide nucleic acid derivatives targeting a 3′ splice site of the human SNAP25 pre-mRNA. The peptide nucleic acid derivatives potently induce at least a splice variant of the human SNAP25 mRNA in cells, and are useful to safely treat dermatological indications or conditions involving the expression of the human SNAP25 protein by topical administration. | 2019-11-07 |
20190338292 | SCN9A ANTISENSE PAIN KILLER - The current invention provides peptide nucleic acid derivatives targeting the 3′ splice site of exon 4 in the human SCN9A pre-mRNA. The peptide nucleic acid derivatives potently induce SCN9A mRNA splice variant(s) lacking the SCN9A exon 4 in cells, and are useful to safely treat pains or conditions involving Na | 2019-11-07 |
20190338293 | HIGH GROWTH CAPACITY AUXOTROPHIC ESCHERICHIA COLI AND METHODS OF USE - This invention provides high growth capacity strains of auxotrophic | 2019-11-07 |
20190338294 | ORTHOGONAL TRANSCRIPTIONAL SWITCHES DERIVED FROM TET REPRESSOR HOMOLOGS FOR SACCHAROMYCES CEREVISIAE - The invention features compositions and methods for identifying orthogonal transcriptional switches derived from Tet repressor homologs for | 2019-11-07 |
20190338295 | MULTI-SUBSTRATE METABOLISM FOR IMPROVING BIOMASS AND LIPID PRODUCTION - The present application relates to methods to improve biomass or lipid production in a microorganism from one or more fatty acid and one or more simple carbon co-substrates. Produced lipids may include unsaturated C | 2019-11-07 |
20190338296 | COMPOSITIONS AND METHODS FOR MODIFYING GENOMES - Compositions and methods for modifying genomic DNA sequences are provided. The methods produce double-stranded breaks (DSBs) at pre-determined target sites in a genomic DNA sequence, resulting in mutation, insertion, and/or deletion of DNA sequences at the target site(s) in a genome. Compositions comprise DNA constructs comprising nucleotide sequences that encode a Cms1 protein operably linked to a promoter that is operable in the cells of interest. The DNA constructs can be used to direct the modification of genomic DNA at pre-determined genomic loci. Methods to use these DNA constructs to modify genomic DNA sequences are described herein. Additionally, compositions and methods for modulating the expression of genes are provided. Compositions comprise DNA constructs comprising a promoter that is operable in the cells of interest operably linked to nucleotide sequences that encode a mutated Cms1 protein with an abolished ability to produce DSBs, optionally linked to a domain that regulates transcriptional activity. The methods can be used to up- or down-regulate the expression of genes at predetermined genomic loci. | 2019-11-07 |
20190338297 | AGROBACTERIUM FOR TRANSIENT TRANSFECTION OF WHOLE PLANTS - A process of transiently transfecting a plant or leaves on a plant, comprising contacting said plant or said leaves with a suspension comprising | 2019-11-07 |
20190338298 | METHOD FOR PRODUCING GENOME-MODIFIED PLANTS FROM PLANT PROTOPLASTS AT HIGH EFFICIENCY - The present invention relates to a method of increasing the production efficiency of gene-edited plants, regenerated from plant protoplasts, by use of a Cas protein-guide RNA ribonucleoprotein (RNP). According to the present invention, the method of increasing the production efficiency of gene-edited plants makes it possible to efficiently produce target gene-mutated plants and to minimize the introduction of foreign DNA into plants. Thus, the present invention can be very advantageously used in a wide variety of fields, including agriculture, food and biotechnology. | 2019-11-07 |
20190338299 | HIGH AMYLOSE WHEAT - III - The present invention provides wheat grain of the species | 2019-11-07 |
20190338300 | MAIZE EVENT DP-004114-3 AND METHODS FOR DETECTION THEREOF - The invention provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-004114-3 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided. | 2019-11-07 |
20190338301 | HIGH LEVEL IN VIVO BIOSYNTHESIS AND ISOLATION OF WATERSOLUBLE CANNABINOIDS IN STABLY TRANSFORMED PLANT SYSTEMS - The inventive technology relates to systems and methods for enhanced in vivo production, accumulation and modification of cannabinoids. In one embodiment, the invention may include systems and methods for enhanced in vivo biosynthesis of chemically-modified water-soluble cannabinoids in stably transformed whole plant, or a cell suspension culture system. | 2019-11-07 |
20190338302 | TRANSGENIC LAND PLANTS COMPRISING A PUTATIVE TRANSPORTER PROTEIN OF AN EDIBLE EUKARYOTIC ALGAE - A transgenic land plant is provided. The transgenic land plant comprises a putative bicarbonate transporter protein of an edible eukaryotic algae. The putative bicarbonate transporter protein of the edible eukaryotic algae is heterologous with respect to the transgenic land plant. The putative bicarbonate transporter protein is an ortholog of CCP1 of | 2019-11-07 |
20190338303 | RESOURCE USE EFFICIENCY IMPROVEMENT IN PLANTS - Polynucleotides and polypeptides incorporated into expression vectors are introduced into plants and were ectopically expressed. These polypeptides may confer at least one regulatory activity and increased yield, increased resource use efficiency, increased water use efficiency, increased light use efficiency, increased photosynthetic capacity, increased photosynthetic rate, increased photosynthetic resource use efficiency, greater vigor, and/or greater biomass as compared to a control plant. | 2019-11-07 |
20190338304 | TRANSCRIPTION REPRESSOR GENE FAMILY AND USES THEREOF - Disclosed are a family of abscisic acid (ABA)-induced transcription repressor genes AITRs and uses thereof for improving plant stress resistance. Mutants with knockout of AITRs genes in | 2019-11-07 |
20190338305 | Fertility Restoration Gene in Wheat and Uses thereof - A fertility restoration gene in wheat and uses thereof, belonging to the field of plant biotechnology, specifically relating to the cloning of a recessive nuclear male sterility gene and a promoter thereof, and uses thereof in hybrid breeding. The fertility restoration gene FRG1 was successfully cloned by flow cytometry and high-throughput sequencing. The FRG1 gene can completely restore the male fertility of a Lanzhou genic male sterile mutant or allelic mutants thereof, which lays a foundation for the construction of a new wheat hybrid breeding technology system, meanwhile, which provides more possibilities to solve the technical bottlenecks of “three lines” and “two lines” hybridization technology of wheat, such as unstable fertility of sterile lines, limited resources for hybrid variety, the complexity of seed production technology and the high cost of seed production. The provided gene and method for propagation and maintenance of the sterile line have important significance and application value for hybrid breeding in wheat. | 2019-11-07 |
20190338306 | SKELETAL MUSCLE CELLS AND METHOD FOR INDUCING SAME - Provided is a method for inducing a skeletal muscle cell including a step of introducing MyoD family gene or an expression product thereof and Myc family gene or an expression product thereof into a somatic cell of a mammal. | 2019-11-07 |
20190338307 | Viral Vector Assay and Vector - A process for assaying viral vector manufactured by large-scale viral vector manufacturing processes to assure the resulting vector has acceptable purity and potency. The process entails three different types of assays, each one of which is optionally useful on a stand-alone basis, and which together provide the first system able to assure the quality of viral vector produced by large-scale vector manufacturing processes. | 2019-11-07 |
20190338308 | Targeted CRISPR Delivery Platforms - The present invention is related to compositions and methods for gene therapy. Several approaches described herein utilize the | 2019-11-07 |
20190338309 | CONTROLLABLE TRANSCRIPTION - The present invention relates to a stable method for introducing at least one inducible cassette into a cell, and permitting controllable transcription from within that inducible cassette. The method may be used for any cell type, from any eukaryotic organism, but has a particular application in the introduction of inducible cassettes into pluripotent stem cells, such as animal or human pluripotent stem cells (hPSCs). The inducible cassette is controllably inserted in such a way to ensure that the genetic material it contains is not silenced or subject to negative influences from the insertion site, and transcription of the genetic material is controlled. | 2019-11-07 |
20190338310 | GENE THERAPY FOR TREATING WILSON'S DISEASE - Compositions and regimens useful in treating Wilson's Disease are provided. The compositions include recombinant adeno-associated virus (rAAV) with a transthyretin enhancer and promoter driving expression of a human ATP7B. | 2019-11-07 |
20190338311 | OPTIMIZED STRATEGY FOR EXON SKIPPING MODIFICATIONS USING CRISPR/CAS9 WITH TRIPLE GUIDE SEQUENCES - CRISPR/Cas9-mediated genome editing holds clinical potential for treating genetic diseases, such as Duchenne muscular dystrophy (DMD), which is caused by mutations in the dystrophin gene. Here, using three promoters to drive expression of the same DMD guide RNA, a more robust and safe form of genome editing was achieved in a humanized mouse model for DMD with a deletion 13 in exon 50, and in a ΔEx50-MD Dog. | 2019-11-07 |
20190338312 | BOCAPARVOVIRUS SMALL NONCODING RNA AND USES THEREOF - Vectors having a nucleotide sequence having SEQ ID NO:1 or a nucleotide sequence having at least 85% identity to SEQ ID NO:1, or a portion thereof, that is capable of regulating bocaparvovirus replication, or vectors having the complement of the nucleotide sequence, and methods of using the vectors, are provided. | 2019-11-07 |
20190338313 | METHODS FOR MAKING AND USING MODIFIED OOCYTES - The present invention provides modified oocytes having a nuclear genome derived from a first oocyte and cytoplasm derived from a second oocyte from a different subject, and methods for making and using such modified oocytes. The methods and compositions of the present invention can be useful in a variety of settings including, but not limited to, in in vitro fertilization (“IVF”) procedures. | 2019-11-07 |
20190338314 | Exosome Delivery System - This disclosure relates to the field of exosome delivery systems. In particular, compositions comprising adipose-derived exosomes that may be used as a delivery system are encompassed. The exosome delivery system can be used to deliver exogenous cargo such as miRNA and other inhibitory RNAs, as well as proteins, to target cells in a subject. | 2019-11-07 |
20190338315 | CLOAKED CRISPRs - A composition including an isolated cloaked gene editor. A composition for treating a lysogenic virus, including a vector encoding isolated nucleic acid encoding two or more cloaked gene editors chosen from cloaked gene editors that target viral DNA, cloaked gene editors that target viral RNA, and combinations thereof. A composition for treating a lytic virus, including a vector encoding isolated nucleic acid encoding at least one cloaked gene editor that targets viral DNA and a cloaked viral RNA targeting composition. A composition for treating both lysogenic and lytic viruses, including a vector encoding isolated nucleic acid encoding two or more cloaked gene editors that target viral RNA. A composition for treating lytic viruses. A method of preventing antibody neutralizing effects with gene editors in humans. Methods of treating a lysogenic virus or a lytic virus, by administering the above compositions to an individual having a virus and inactivating the virus. | 2019-11-07 |
20190338316 | RECOMBINANT MICROORGANISM HAVING HETEROLOGOUS GENES INTRODUCED THERETO AND METHOD FOR PRODUCING USEFUL MATERIAL FROM FORMIC ACID AND CARBON DIOXIDE USING SAME MICROORGANISM - The present invention relates to a recombinant microorganism having heterologous genes introduced thereto and a method for producing a useful material from formic acid and carbon dioxide using the microorganism. The present invention provides a novel microorganism having a cyclic metabolic pathway introduced thereto through which C3 or higher carbon organic compounds can be synthesized from formic acid and carbon dioxide, whereby carbon dioxide rich in nature and formic acid that is of low toxicity and suitable for anabolic reaction in view of reaction kinetics and which can be easily and rapidly synthesized from carbon dioxide can be used to effectively synthesize the C3 organic compound pyruvic acid from which various high-value added compound can be synthesized. | 2019-11-07 |
20190338317 | MICROORGANISMS FOR THE PRODUCTION OF INSECT PHEROMONES AND RELATED COMPOUNDS - The present application relates to recombinant microorganisms useful in the biosynthesis of unsaturated C | 2019-11-07 |
20190338318 | Method of Producing Fatty Alcohol - A method of producing a long-chain fatty alcohol, containing culturing a microorganism wherein expression of a gene encoding a 3-ketoacyl-ACP synthase and expression of a gene encoding a fatty acyl-CoA reductase are enhanced;
| 2019-11-07 |
20190338319 | Improved Yeast For Ethanol Production - Described herein are processes for producing ethanol from starch-containing material using a | 2019-11-07 |
20190338320 | MATERIALS AND METHODS FOR CONTROLLING PHA BIOSYNTHESIS IN PHA-GENERATING SPECIES OF THE GENERA RALSTONIA OR CUPRIAVIDUS AND ORGANISMS RELATED THERETO - Provided herein are methods for generating cellular biomass in continuous aerobic fermentation systems. The biomass yield, and the concentration of polyhydroxyalkanoate within the biomass, are each directed to advantageous levels by operating the continuous fermentation system under particular nutrient limitation conditions. Also provided are biomass produced using the provided methods, and animal feed compositions including the provided biomass. | 2019-11-07 |
20190338321 | Host Cell Modified to Produce Lactams - The present invention provides for a genetically modified host cell capable of producing a lactam comprising a 2-pyrrolidone synthase, or an enzymatically active fragment thereof, heterologous to the host cell. | 2019-11-07 |
20190338322 | POLYPEPTIDE HAVING THE ACTIVITY OF EXPORTING O-ACETYL-HOMOSERINE - The present disclosure relates to a protein having the activity of exporting O-acetylhomoserine and a novel modified protein thereof, a microorganism capable of producing O-acetylhomoserine with enhanced expression of the protein, and a method for producing O-acetylhomoserine using the microorganism. | 2019-11-07 |
20190338323 | EXPRESSION OF RECOMBINANT TETRACYCLINE EFFLUX PUMPS FOR THE PRODUCTION OF LYSINE OR LYSINE-DERIVED PRODUCTS, AND METHODS AND APPLICATIONS THEREOF - One aspect of the invention relates to a mutant polypeptide comprising the amino acid sequence of | 2019-11-07 |
20190338324 | PROCESS FOR PRODUCING L-METHIONINE FROM METHIONAL - A method is useful for the biocatalytic synthesis of proteinogenic L-amino acids, such as L-alanine, L-valine. L-methionine. L-leucine, L-isoleucine or L-phenylalanine from a respective aldehyde and carbon dioxide. In particular, the method is useful for the biocatalytic synthesis of L-methionine from 3-methylthio-propanal (“methional”) and carbon dioxide. | 2019-11-07 |
20190338325 | FUNCTIONALISED POLYSULPHIDE SYNTHESIS METHOD - Provided is process for the synthesis of at least one functionalized organic polysulfide. | 2019-11-07 |
20190338326 | METHODS FOR PRODUCING CARBO SUGARS AND APPLICATIONS THEREOF - Described herein are biological devices and methods for using the same to produce carbo sugars. The biological devices include microbial cells transformed with a DNA construct containing genes for producing a cellulose synthase and galactomannan galactosyltransferase. In some instances, the biological devices also include a gene for lipase. Methods for altering the viscosity of petroleum oil using the carbo sugars are also described herein. Finally, methods for degreasing or decontaminating water mixed with petroleum oil or other fatty substances or a surface coated with petroleum oil or other fatty substances using the carbo sugars are described herein. | 2019-11-07 |
20190338327 | A PROCESS FOR PRODUCTION OF GALACTO-OLIGOSACCHARIDES - A process for production of galacto-oligosaccharides is provided. The process for production of galacto-oligosaccharides includes growing | 2019-11-07 |
20190338328 | IN VIVO SYNTHESIS OF SIALYLATED COMPOUNDS - The present invention is in the technical field of synthetic biology and metabolic engineering. More particularly, the present invention is in the technical field of fermentation of metabolically engineered microorganisms. The present invention describes engineered microorganisms able to synthesize sialylated compounds via an intracellular biosynthesis route. These microorganisms can dephosphorylate N-acetylglucosamine-6-phosphate to N-acetylglucosamine and convert the N-acetylglucosamine to N-acetylmannosamine. These microorganisms also have the ability to convert N-acetylmannosamine to N-acetyl-neuraminate. Furthermore, the present invention provides a method for the large scale in vivo synthesis of sialylated compounds, by culturing a microorganism in a culture medium, optionally comprising an exogenous precursor such as, but not limited to lactose, lactoNbiose, N-acetyllactosamine and/or an aglycon, wherein said microorganism intracellularly dephosphorylates N-acetylglucosamine-6-phosphate to N-acetylglucosamine, converts N-acetylglucosamine to N-acetylmannosamine and convert the latter further to N-acetyl-neuraminate. | 2019-11-07 |
20190338329 | HEXURONATE C4-EPIMERASE VARIANT HAVING IMPROVED D-TAGATOSE CONVERSION ACTIVITY, AND D-TAGATOSE PRODUCTION METHOD USING SAME - Provided are a hexuronate C4-epimerase variant with improved activity in converting D-fructose by D-tagatose of hexuronate C4-epimerase and a method for production of D-tagatose using them. | 2019-11-07 |
20190338330 | Method for Producing Heparosan Compound Having Isomerized Hexuronic Acid Residue - The present invention provides a method for producing a heparosan compound having an isomerized hexuronic acid residue and a method for producing a heparan sulfate with improved C5-epimerization efficiency. Specifically, the present invention provides a method for producing a heparosan compound having an isomerized hexuronic acid residue, said method comprising producing the heparosan compound having the isomerized hexuronic acid residue from a heparosan compound in the presence of a protein selected from the group of consisting of the following (A) to (F): (A) a protein comprising the amino acid sequence of SEQ ID No:2; (B) a protein which comprises an amino acid sequence having 90% or more homology to the amino acid sequence of SEQ ID No:2 and has a D-glucuronyl C5-epimerase substituted, added or inserted amino acid residues in the amino acid sequence of SEQ ID No:2 and has a D-glucuronyl C5-epimerase activity; (D) a protein comprising the amino acid sequence of SEQ ID NO:5; (E) a protein which comprises an amino acid sequence having 90% or more homology to the amino acid sequence of SEQ ID No:5 and has a D-glucuronyl C5-epimerase activity; and (F) a protein which comprises an amino acid sequence having one or several deleted, substituted, added or inserted amino acid residues in the amino acid sequence of SEQ ID No:5 and has a D-glucuronyl C5-epimerase activity. | 2019-11-07 |
20190338331 | Nucleotide Triphosphate Immobilised on a Support and Their Use in Nucleic Acid Synthesis - The invention relates to methods of synthesizing polymers, biopolymers, and nucleic acids, to immobilised dNTP/NTPs and kits comprising said immobilised dNTP/NTPs for use in said methods of nucleic acid synthesis. | 2019-11-07 |
20190338332 | ENZYMATIC METHOD FOR PREPARING REBAUDIOSIDE J - Provided is a method for preparing Rebaudioside J using an enzymatic method, comprising using rebaudioside A as a substrate, and making the substrate, in the presence of a glycosyl donor, react under the catalysis of a UDP-glycosyltransferase-containing recombinant cell and/or UDP-glycosyltransferase prepared therefrom to generate Rebaudioside J. | 2019-11-07 |
20190338333 | 7-Beta-hydroxysteroid Dehydrogenase Mutants and Process for the Preparation of Ursodeoxycholic Acid - The invention relates to novel 7β-hydroxysteroid dehydrogenase mutants, to the sequences which code for these enzyme mutants, to processes for the preparation of the enzyme mutants and to their use in enzymatic conversions of cholic acid compounds, in particular in the preparation of ursodeoxycholic acid (UDCA); subject-matter of the invention is also novel processes for the synthesis of UDCA using enzyme mutants; and the preparation of UDCA using recombinant, multiply modified microorganisms. | 2019-11-07 |
20190338334 | Methods for Rapid Determinations of Antibiotic Susceptibility Phenotypes - The invention is directed to a method for rapidly determining antibiotic susceptibility in bodily fluid samples based on rigorous multidimensional statistical metrics. In some embodiments, the method incorporates flow cytometry to determine susceptibility. In preferred embodiments, the method is adapted for use with samples with low bacterial counts. | 2019-11-07 |
20190338335 | Article and Methods to Determine Efficacy of Disinfection Process - An article is provided. The article comprises a nonwoven substrate having a copolymer grafted thereto, and a dried coating adhered to the substrate. The copolymer comprises interpolymerized monomer units of a quaternary ammonium-containing ligand monomer, an amide monomer, and an oxy monomer. The coating comprises a plurality of test microorganisms. Optionally, the coating further comprises a water-soluble or water-dispersible polymeric binding agent. A process challenge device comprising a body having a hollow channel with said article fixed disposed therein is also provided. Methods of using the article or the process challenge device for determining the efficacy of a disinfection process are also provided. | 2019-11-07 |
20190338336 | COMPOUNDS AND METHODS FOR DETECTION OF ENZYMES THAT REMOVE FORMYL, SUCCINYL, METHYL SUCCINYL OR MYRISTOYL GROUPS FROM EPSILON-AMINO LYSINE MOIETIES - Provided is a compound that comprises the structure: | 2019-11-07 |
20190338337 | ACETAMINOPHEN ASSAY - Disclosed herein is an assay for determining the concentration of p-aminophenol present in a sample. More particularly, the present invention relates to an improvement of an enzyme-based assay for determining the concentration of acetaminophen present in a sample. | 2019-11-07 |
20190338338 | NOVEL USES OF CATALYTIC PROTEIN - The present invention relates to a method of enriching or screening for one or more target molecules from a primary source, which method comprises to provide at least one peptidic ligand comprising at least one lysine (K) and immobilized to a solid support; contacting the ligand(s) with a primary source comprising at least one target molecule comprising glutamine (Q); allowing the formation of complexes between the ligand and the target molecule; and separating the complexes from the primary source. The target molecule(s) comprises glutamine, and step c is performed in the presence of a catalytic protein comprising transglutaminase (TG). The catalytic protein comprising transglutaminase (TG) may comprise transglutaminase originating from fish, such as Atlantic cod TG (AcTG), e.g. AcTG-1, and the primary source may include waste material from the fish or dairy industry. | 2019-11-07 |
20190338339 | PLASMA DEPOSITED ADHESION PROMOTER LAYERS FOR USE WITH ANALYTE SENSORS - Embodiments of the invention provide methods and materials for making analyte sensors having a plurality of layered elements such as amperometric glucose sensors that are used by diabetic individuals to monitor blood sugar concentrations. Embodiments of the invention utilize plasma deposition technologies to form thin films of adhesion promoting compositions useful in such sensors. Sensors that incorporate the thin film compositions formed by these processes exhibit a number of desirable characteristics. | 2019-11-07 |
20190338340 | 5,5-DISUBSTITUTED LUCIFERINS AND THEIR USE IN LUCIFERASE-BASED ASSAYS - Described are luciferin analogues, methods for making the analogues, kits including the analogues, and methods of using the compounds for the detection of luminescence in luciferase-based assays. | 2019-11-07 |
20190338341 | Methods and device for electromagnetic detection of polymerase chain reaction - In a sample—reagent mix for polymerase chain reaction (PCR), forward primers are attached to superparamagnetic beads. During a part of a cycle of PCR, beads with bound amplicons are attracted to an electromagnet coil. Electrodeless, wireless detection of PCR and nucleic acid quantitation are achieved in real time by placing the beads in the ac electromagnetic field of the same, and/or another, coil and measuring, with one or both coils, cycle-by-cycle changes in the electrical conductivity and/or the complex permittivity of the aggregate of beads, amplicons, and the interspersed reaction mix. After the cycle's measurement is complete, the beads are redispersed within the reaction mix by coordinated action of the first coil and a third coil. | 2019-11-07 |
20190338342 | SYSTEMS AND METHODS FOR ENHANCED SCODA - Methods and apparatus for separating, concentrating and/or detecting molecules based on differences in binding affinity to a probe are provided. The molecules may be differentially modified. The molecules may be differentially methylated nucleic acids. The methods can be used in fields such as epigenetics or oncology to selectively concentrate or detect the presence of specific biomolecules or differentially modified biomolecules, to provide diagnostics for disorders such as fetal genetic disorders, to detect biomarkers in cancer, organ failure, disease states, infection or the like. | 2019-11-07 |
20190338343 | MULTIPLEX NUCLEIC ACID AMPLIFICATION - In some embodiments, the disclosure relates generally to compositions, comprising a single reaction mixture containing a plurality of different populations of discrete supports, and a plurality of different populations of target nucleic acids. The single reaction mixture can contain a first population of beads; a second population of beads; a first population of target nucleic acids, where at least two different target nucleic acids in the first population of target nucleic acids can bind to a bead in the first population of beads; and a second population of target nucleic acids, where at least two different target nucleic acids in the second population of target nucleic acids can bind to a bead in the second population of beads. The single reaction mixture can be employed to monoclonally amplify the first target nucleic acids on the first beads, and monoclonally amplify the second target nucleic acids on the second beads. | 2019-11-07 |
20190338344 | METHOD FOR PROCESSING POLYNUCLEOTIDE-CONTAINING SAMPLES - Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that retain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed. | 2019-11-07 |
20190338345 | GENERATING CELL-FREE DNA LIBRARIES DIRECTLY FROM BLOOD - The disclosure provides methods and kits for preparing sequencing library to detect chromosomal abnormality using cell-free DNA (cfDNA) without the need of first isolating the cfDNA from a liquid fraction of a test sample. In some embodiments, the method involves reducing the binding between the cfDNA and nucleosomal proteins without unwinding the cfDNA from the nucleosomal proteins. In some embodiments, the reduction of binding may be achieved by treating with a detergent or heating. In some embodiments, the method further involves freezing and thawing the test sample before reducing the binding between the cfDNA and the nucleosomal proteins. In some embodiments, the test sample is a peripheral blood sample from a pregnant woman including cfDNA of both a mother and a fetus. In other embodiments, the test sample is a peripheral blood sample from a patient known or suspected to have cancer. | 2019-11-07 |
20190338346 | METHOD AND SYSTEM FOR SAMPLE PREPARATION - A method for preparing a sample by utilizing a shearing force in the presence of a size stabilizer to break apart the sample to obtain nucleic acid molecules in a usable size range. Once nucleic acid molecules are obtained, magnetic nanoparticles are used to concentrate and clean the nucleic acid molecules for further testing. | 2019-11-07 |
20190338347 | NICKING AND EXTENSION AMPLIFICATION REACTION (NEAR) OF STREPTOCOCCUS SPECIES - This invention relates to compositions, methods and kits for detecting the presence or absence of a bacterial species in a biological sample using isothermal nucleic acid amplification. | 2019-11-07 |
20190338348 | ISOTHERMAL METHODS FOR AMPLIFYING NUCLEIC ACID SAMPLES - The description provides two-stage methods of nucleic acid amplification and detection reactions, which are useful for rapid pathogen detection or disease diagnosis. In particular, the description provides a method comprising a first-stage slow rate amplification reaction followed by a plurality of second-stage fast rate amplification reactions that are simultaneously monitored in real-time, and wherein a rapid rate of amplification is indicative of the presence of a site of interest. | 2019-11-07 |
20190338349 | METHODS AND SYSTEMS FOR HIGH FIDELITY SEQUENCING - Systems and methods for high fidelity sequencing and identification of rare mutations at dilute concentrations in a sample are described herein. In various aspects, the use of specialized library preparation techniques including adapter ligation conditions and hybrid capture enrichment panels are used along with controls to increase yield of sequence-ready molecules and identify and minimize contamination and errors. Systems and methods also relate to analyzing sequencing data to differentiate true variants from false positives using ensembles and a quasi-maximum likelihood model. | 2019-11-07 |
20190338350 | Method, device and kit for detecting fetal genetic mutation - Provided are a method and a device for detecting a genetic mutation, and a kit for typing genotypes of a pregnant woman and a fetus. The method comprises: performing high-throughput sequencing on free DNA in a pregnant woman's peripheral blood to obtain sequencing data; comparing the sequencing data with reference genome to obtain SNP sites; performing mixed genotyping on each SNP site to obtain target genotypes for each SNP site; and selecting a mutation site that causes the gene mutation from the genotype of the fetus in the target genotypes. | 2019-11-07 |
20190338351 | METHODS OF BEAD MANIPULATION AND FORMING BEAD ARRAYS - According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same. | 2019-11-07 |
20190338352 | ACCURACY OF BASE CALLS IN NUCLEIC ACID SEQUENCING METHODS - A method of determining nucleic acid sequences can include steps of (a) obtaining signal data from a nucleic acid sequencing procedure carried out on an array of nucleic acid features; (b) extracting signals from each nucleic acid feature to produce multiple extracted signal traces that each correlate signal characteristics with sequencing cycle for a particular nucleotide type at a particular nucleic acid feature; (c) comparing the series of signals for different nucleotide types at each of the features to distinguish a candidate base call from background signals for each cycle at each feature; (d) applying a baseline adjustment to each series of signals based on the extracted background signals; and (e) comparing the adjusted signal traces for different nucleotide types at each of the features, thereby distinguishing adjusted signals having characteristics of a base call from adjusted background signals for each cycle at each feature. | 2019-11-07 |
20190338353 | METHODS AND SYSTEMS FOR PROCESSING POLYNUCLEOTIDES - The present disclosure provides compositions, methods, systems, and devices for polynucleotide processing and analyte characterization. Such polynucleotide processing may be useful for a variety of applications, including analyte characterization by polynucleotide sequencing. The compositions, methods, systems, and devices disclosed herein generally describe barcoded oligonucleotides, which can be bound to a bead, such as a gel bead, useful for characterizing one or more analytes including, for example, protein (e.g., cell surface or intracellular proteins), genomic DNA, and RNA (e.g., mRNA or CRISPR guide RNAs). Also described herein, are barcoded labelling agents and oligonucleotide molecules useful for “tagging” analytes for characterization. | 2019-11-07 |
20190338354 | NUCLEIC ACID TAGGANTS - The present disclosure relates to compositions and methods for marking/tagging objects for identification. In particular, tagging objects with a nucleic acid taggant (genetic tag based merchandise authentication). | 2019-11-07 |