45th week of 2014 patent applcation highlights part 36 |
Patent application number | Title | Published |
20140329229 | ABSORBENT DRIED BIOFLUID COLLECTION SUBSTRATES - A new device and methods that allow for improved sequestration and preservation of harvested analytes and bio-molecules from biofluid samples is defined. The new device and methods relate to an improved dried biofluid collection substrate that is absorbent and contains a plurality of affinity ligands located within defined sample collection regions for enhanced analyte collection and storage. The device and methods allow for simple, safe and reliable ambient temperature collection and preservation of molecules captured from biological and environmental fluids in quantities suitable for analysis and diagnostic testing. | 2014-11-06 |
20140329230 | METHODS FOR DETECTING HUMAN PAPILLOMAVIRUS AND PROVIDING PROGNOSIS FOR HEAD AND NECK SQUAMOUS CELL CARCINOMA - Methods and kits for diagnosing and determining prognosis of head and neck squamous cell carcinoma are described. An exemplary method for diagnosing head and neck squamous cell carcinoma (HNSCC) in a subject may comprise assaying a saliva sample from the subject for the presence of total protein, solCD44, and HPV, and using the combination of total protein, HPV, and CD44 levels in a multivariate analysis to determine a combined score, whereby an increase in score above a cut-off point distinguishes subjects with HNSCC, or an elevated risk of future occurrence thereof, from subjects without HNSCC or at low risk of future occurrence thereof. | 2014-11-06 |
20140329231 | LIQUID MEDIUM AND SAMPLE VIAL FOR USE IN A METHOD FOR DETECTING CANCEROUS CELLS IN A CELL SAMPLE - The current invention concerns a method for determining the presence of a (pre)cancerous cell in a liquid cell sample, comprising the steps of: —suspending and preserving a cell sample obtained from a subject in a sample vial comprising a liquid medium, said liquid medium comprises means for labeling cells or epitope(s) on or in said cells; —obtaining data from said labelled liquid cell sample; and —determining the presence of said (pre)cancerous cells based on said obtained data; characterized in that said data comprises morphological data and biomarker data. In a further aspect, the invention relates to a liquid medium for fixing, preserving and labeling cells in a cell sample and a sample vial specifically designed to be used in conjunction with the current method. | 2014-11-06 |
20140329232 | METHOD FOR THE DETECTION OF NUCLEIC ACID SYNTHESIS AND/OR AMPLIFICATION - The present invention relates to a method for the detection of nucleic acid synthesis and/or amplification, characterised in that the method includes adding at least one colorimetric metal indicator and at least one bland magnesium chelator to a reaction mixture for nucleic acid amplification. The present invention further relates to a kit for carrying out such a method and to the use thereof in the health, food and agricultural or veterinary fields. | 2014-11-06 |
20140329233 | METHODS, COMPOSITIONS AND KITS FOR A ONE-STEP DNA CLONING SYSTEM - Methods and kits for joining two or more polynucleotides to form a product polynucleotide are provided. A mixture contains a first polynucleotide comprising a selectable marker. The mixture further contains a second polynucleotide comprising a first typeIIs recognition sequence and a second typeIIs recognition sequence. The second polynucleotide is other than the first polynucleotide. The mixture further contains a first typeIIs restriction endonuclease that cleaves the first typeIIs recognition sequence to produce a first end, a second typeIIs restriction endonuclease that cleaves the second typeIIs recognition sequence to produce a second end, and a DNA ligase. The first end is not compatible with the second end. The combined actions of the enzymes in the mixture join the first polynucleotide to the second polynucleotide forming a product polynucleotide, which is obtained by transforming the mixture into a host cell. | 2014-11-06 |
20140329234 | FLUORESCENT LABELING OF TRANSFER RNA AND STUDY OF PROTEIN SYNTHESIS - Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system. The disclosed methods are useful in single molecule as well as in ensemble settings. | 2014-11-06 |
20140329235 | Sensor Housing and Reagent Chemistry - A sensor comprises a sensor housing, having a channel; a porous substrate, in the channel; an analysis chemistry reagent, on the porous substrate; and a nozzle, in fluid connection with the channel. The porous substrate fills a cross section of the channel, and the cross-sectional area of the channel at the porous substrate is greater than the cross-sectional area at the nozzle. | 2014-11-06 |
20140329236 | NON-NATURAL NUCLEOSIDES AS THERANOSTIC AGENTS - A theranostic agent for inhibiting translesion DNA replication comprises natural adenine ribose analog having formula I. | 2014-11-06 |
20140329238 | DETECTION OF GENE DUPLICATIONS - Methods of detecting a candidate genetic anomaly such as a candidate duplication in a genome are disclosed. The methods comprise quantifying fluorogenic assays for alleles of a genetic locus from a plurality of individual genomes, identifying ranges of fluorescent intensities indicative of individual genomes homozygous for a first allele, homozygous for a second allele, or heterozygous for both alleles, and identifying individual genomes in which the fluorescence intensities are outside the range of intensities indicative of homozygosity or heterozygosity for the genetic locus. | 2014-11-06 |
20140329239 | DIGITAL ANALYTE ANALYSIS - The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons. | 2014-11-06 |
20140329240 | CHIP-BASED SEQUENCING NUCLEIC ACIDS - A system for fast DNA sequencing by amplification of genetic material within microreactors, denaturing, demulsifying, and then sequencing the material, while retaining it in a PCR/sequencing zone by a magnetic field. One embodiment includes sequencing nucleic acids on a microchip that includes a microchannel flow channel in the microchip. The nucleic acids are isolated and hybridized to magnetic nanoparticles or to magnetic polystyrene-coated beads. Microreactor droplets are formed in the microchannel flow channel. The microreactor droplets containing the nucleic acids and the magnetic nanoparticles are retained in a magnetic trap in the microchannel flow channel and sequenced. | 2014-11-06 |
20140329241 | HYDROLASE ENZYME SUBSTRATES AND USES THEREOF - The present invention provides novel methods for determining the presence or amount of a hydrolytic enzyme in a sample, based on novel substrates for the enzymes, and also provides compositions and methods that provide highly sensitive assay methods for such hydrolytic enzymes. | 2014-11-06 |
20140329242 | CHARACTERIZING MULTIPLE SCLEROSIS - A method for characterizing multiple sclerosis in a subject involves comparing ratios of expression levels of genes in a biological sample from a subject to references, wherein the multiple sclerosis is characterized based on a difference in the ratios of the expression values of genes in the biological sample from the subject as compared to the references. | 2014-11-06 |
20140329243 | METHOD FOR CHARACTERIZING CIRCULATING TUMOR CELLS, AND USE THEREOF IN DIAGNOSIS - Disclosed is a method for characterization, in a biological sample, of circulating tumour cells (CTCs) bearing at least one marker characteristic of the tumorous nature of the cell, the marker being selected from the groups constituted by: the oncogenic proteins characteristic of the CTCs, with the exception of the proteins encoded by the EML4-ALK fusion gene, and the tumour markers. Also disclosed is the use of this method for deciding on the implementation of a treatment for a cancer patient. | 2014-11-06 |
20140329244 | APPARATUS FOR AMPLIFICATION OF NUCLEIC ACIDS - Described herein is a chip-based apparatus for amplifying nucleic acids, a cartridge housing the apparatus, and methods of using the apparatus for amplification of nucleic acids. More specifically, this invention provides integrated semiconductor chip, manufactured with standard semiconductor manufacturing process, with on-chip circuitry to perform thermal management and optical sensing necessary for amplification of nucleic acids. The apparatus and methods embodied in this invention makes it possible to build a disease diagnosis and prognosis tool that is easy to use, portable and disposable. | 2014-11-06 |
20140329245 | Methods and Compositions for PCR Using Blocked and Universal Primers - Provided herein are methods and compositions for performing PCR with primers with blocked 3′-ends that are unblocked when these primers anneal to the template. The multiplexed PCR can be used as real-time qPCR, for end-point detection or as enrichment method for next generation sequencing (NGS). Also described herein are methods and compositions to improve sensitivity of mutation-specific PCR when targeting closely-spaced mutations. | 2014-11-06 |
20140329246 | AMPLIFICATION METHODS TO MINIMISE SEQUENCE SPECIFIC BIAS - Methods for amplifying nucleic acids are provided. The methods can be used to minimise sequence specific bias caused by the preferential amplification of certain nucleic acid sequences. Methods are described which can lower the efficiency of AT rich templates relative to GC rich templates, thereby minimising GC bias during amplification reactions with multiple templates of different sequence. The methods are suited to solid phase amplification, for example, utilising flow cells. | 2014-11-06 |
20140329247 | METHODS FOR MULTIPLEXING AMPLIFICATION REACTIONS - A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify | 2014-11-06 |
20140329248 | B7-H1 AND SURVIVIN IN CANCER - Methods of determining prognosis of a subject with cancer by assessing expression of B7-H1 and survivin in combination. | 2014-11-06 |
20140329249 | SELECTIVE RELEASE OF SUB-GROUP OF BIOLOGICAL UNITS - A method of individually releasing from an entity one or more members of a sub-group of biological units included in a heterogeneous group of biological units is provided. The method includes binding the group of biological units including the sub-group of biological units to the entity via a linker. Following binding, the location of the one or more members on the entity is determined. Once the location is determined, a localized physical pulse is applied to the one or more members. The localized physical pulse individually releases the one or more members from the entity by dissociating the linker. | 2014-11-06 |
20140329250 | CALCIUM-BINDING PHOTOPROTEIN - The invention provides calcium-binding photoproteins which can detect light emission with a higher sensitivity. The proteins of the invention comprising the amino acid sequence of SEQ ID NO: 2 can be used for the detection and measurement of calcium ions. The proteins of the invention are useful as reporter proteins, luminescent markers, etc. The polynucleotides of the invention are useful as reporter genes, etc. | 2014-11-06 |
20140329251 | LTBP2 AS A BIOMARKER FOR LUNG INJURY - The application discloses LTBP2 as a new biomarker for pulmonary injury; methods for the diagnosis, prediction, prognosis and/or monitoring of said pulmonary injury based on measuring said biomarker; and kits and devices for measuring said biomarker and/or performing said methods. | 2014-11-06 |
20140329252 | METHODS AND KITS FOR DIAGNOSING AND/OR PROGNOSING OSTEOARTHRITIS - A method of determining whether a subject is at risk of developing osteoarthritis (OA), said method comprising: determining the cellular localization of a Prohibitin-1 (PHB1) polypeptide and/or Small Ubiquitin-like Modifier (SUMO) polypeptide and/or UBC9, in a cell sample from said subject; and determining whether said subject is at risk of developing OA based on the cellular localization of a PHB1 polypeptide and/or SUMO and/or UBC9 polypeptide, is described. | 2014-11-06 |
20140329253 | Monoclonal Antibody For The Detection of SNAP/CLIP Tag - A monoclonal antibody that binds specifically to the SNAP motif and to the CLIP tag comprising CDRs with the amino acid sequences SEQ ID Nos. 3, 4, 5, and 8, 9, 10. | 2014-11-06 |
20140329254 | BIOSENSOR USING REDOX CYCLING - The present invention relates to a biosensor using dual amplification of signal amplification by means of enzymes coupled with signal amplification by means of redox cycling, and to the use of a technology that maintains a slow reaction state between redox cycling materials without the use of redox enzymes, and induces quick chemical-chemical redox cycling. In addition, the present invention relates to a biosensor which obtains triple amplification by inducing electrochemical-chemical-chemical redox cycling, in addition to signal amplification by means of enzymes and signal amplification by means of chemical-chemical redox cycling. | 2014-11-06 |
20140329255 | METHODS OF SCREENING T1R1/T1R3 RECEPTORS FOR COMPOUNDS THAT MODULATE UMAMI TASTE SIGNALING - The present invention relates to the discovery that the T1R receptors assemble to form functional taste receptors. Particularly, it has been discovered that co-expression of T1R1 and T1R3 results in a taste receptor that responds to umami taste stimuli, including monosodium glutamate. Also, it has been discovered that co-expression of the T1R2 and T1R3 receptors results in a taste receptor that responds to sweet taste stimuli including naturally occurring and artificial sweeteners. | 2014-11-06 |
20140329257 | DETECTION OF HYDROGEN PEROXIDE - The present invention provides compounds useful for detection of hydrogen peroxide and methods of using same. | 2014-11-06 |
20140329258 | SELF-CONTAINED TEST SENSOR - A test strip to assist in determining the concentration of an analyte in a fluid sample comprises a base, at least one tab and a break line. The base includes a capillary channel and a test element. The capillary channel is in fluid communication with the test element. The test element is adapted to receive the fluid sample. The at least one tab is removably attached to the base. The capillary channel extends from the base into a portion of the tab. The break line intersects the capillary channel in which an inlet to the capillary channel is exposed along the break line when the tab is separated from the base. | 2014-11-06 |
20140329259 | Systems and Methods of Sample Processing and Fluid Control in a Fluidic System - This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications. | 2014-11-06 |
20140329260 | METHOD OF DIAGNOSIS OF FIBROTIC DISEASES - The present invention relates to a new diagnosis method in the field of hepatology, combining measurement of serum markers and of liver elasticity. | 2014-11-06 |
20140329261 | TREATMENT OF CANCER BY INHIBITION OF THE MYD88/ERK MAP KINASE INTERACTION - A method for selecting in vitro compounds is capable of potentiating the effect of a DNA damage inducing chemotherapy agent for the treatment of cancer, and includes selecting compounds inhibiting the interaction between MyD88 and ERK MAP KINASE. | 2014-11-06 |
20140329262 | TESTING SYSTEM AND METHOD FOR TESTING - This invention relates to a testing system for assessing the level of a biochemical marker, comprising a disposable device ( | 2014-11-06 |
20140329263 | Stabilization of Labile Analytes in Reference Materials - Provided herein are assay control materials comprising stable analytes and lyophilized unstable analytes, and methods of making and using the same. | 2014-11-06 |
20140329264 | FIBROBLAST GROWTH PATTERNS FOR DIAGNOSIS OF ALZHEIMER'S DISEASE - Methods of diagnosing Alzheimer's disease are provided. At least five methods of diagnostic measurements are presented: Method 1: Integrated score; Method 2: Average aggregate area per number of aggregates; Method 3: Cell migration analysis; Method 4: Fractal analysis; Method 5: Lacunarity Analysis. In certain embodiments, a sample of a subject's skin provides a network of fibroblasts that is imaged and a fractal dimension of the image is calculated. The fractal dimension can be compared to an aged-matched control (non-Alzheimer's) database to determine if the subject has Alzheimer's disease. The network of fibroblasts may be cultured in a matrix, for example in a protein mixture. | 2014-11-06 |
20140329265 | FLOWCELL, SHEATH FLUID, AND AUTOFOCUS SYSTEMS AND METHODS FOR PARTICLE ANALYSIS IN URINE SAMPLES - The present disclosure relates to apparatus, systems, compositions, and methods for analyzing a sample containing particles. A particle imaging system or analyzer can include a flowcell through which a urine sample containing particles is caused to flow, and a high optical resolution imaging device which captures images for image analysis. A contrast pattern for autofocusing is provided on the flowcell. The image processor assesses focus accuracy from pixel data contrast. A positioning motor moves the microscope and/or flowcell along the optical axis for autofocusing on the contrast pattern target. The processor then displaces microscope and flowcell by a known distance between the contrast pattern and the sample stream, thus focusing on the sample stream. Cell or particle images are collected from that position until autofocus is reinitiated, periodically, by input signal, or when detecting temperature changes or focus inaccuracy in the image data. | 2014-11-06 |
20140329266 | CERAMIDE DERIVATIVE AND GOLGI APPARATUS-LABELING FLUORESCENT PROBE USING SAME - A measure for selective fluorescent labeling of the Golgi apparatus, which has low cytotoxicity and excellent intracellular stability, is provided. According to the present invention, provided are a ceramide derivative represented by the following Chemical formula 1: | 2014-11-06 |
20140329267 | SELF-HEALING MATERIAL AND METHOD FOR THE PREPARATION THEREOF - It is provided a self-healing polymer network comprising transition metal thiolates, particularly thiolates of a transition metal that is able to self-assemble by metallophilic attractions, and more particularly Au(1), Ag(1), Cu(1) thiolates, or a mixture thereof, and, optionally, disulfide bonds, thiol and other thiolate groups. It is also provided several processes for the preparation of the self-healing polymer networks of the invention, as well as uses thereof. | 2014-11-06 |
20140329268 | ARRANGEMENT FOR DETECTION OF HEMOLYSIS - The following invention relates to a device for visual detection of hemolysis in a whole blood sample from a pierceable container, said device comprising at least one visible detection compartment and a transfer passage connected to said visible detection compartment, said device further comprising means for passing through the container to the interior of said container for accessing the whole blood and permitting transfer of a volume of plasma from said sample to said detection compartment via said transfer passage, wherein said device further is arranged with a separation device for separating plasma from blood cells within said whole blood sample before said plasma reaches the detection compartment, said device further being arranged with means providing a capillary action for generating a capillary force urging said volume of plasma to be transferred through the separation device to said detection compartment. | 2014-11-06 |
20140329269 | DEVICES, SYSTEMS, AND METHODS FOR EXTRACTING A MATERIAL FROM A MATERIAL SAMPLE - Devices, systems, and associated methods for selectively extracting a material from a sample are provided. In one aspect, for example, a method for selectively extracting biological material from a biological sample can include identifying a region of biological material to be extracted from a biological sample disposed on a substantially planar surface, applying an extraction tool to the region of biological material to disrupt biological material from the biological sample, and dispensing a liquid at the region of biological material. The method can also include aspirating the liquid and the disrupted biological material from the biological sample. | 2014-11-06 |
20140329270 | AUTOMATED SYSTEM AND METHOD OF TREATING TISSUE SAMPLES ON SLIDES - An automated method and system for treating one or more tissue samples disposed on slides, the system comprising: a controller, a plurality of slide treatment modules arranged to receive ones of the slides; at least one fluid dispensing robot configured by the controller to dispense a plurality of reagents to said ones of the slides received in the slide treatment modules via an output nozzle disposed on the at least one fluid dispensing robot to treat said one or more tissue samples respectively; and at least one pumping means for pumping said reagents to the output nozzle of the at least one fluid dispensing robot from a plurality of reagent containers comprising said reagents, wherein the at least one fluid dispensing robot is configured by the controller to dispense said reagents in a predetermined sequence for each of the slide treatment modules to treat the one or more tissue samples disposed on each of the slides independently. | 2014-11-06 |
20140329271 | SCREENING FOR NITROGEN FIXATION - An essentially glutamine-free medium comprising a whole-cell glutamine biosensor, comprising a glutamine auxotrophic | 2014-11-06 |
20140329272 | Metal-Based Covalent Viability Reagent for Single Cell Analysis - The present invention provides a robust viability stain for methods utilizing elemental analysis. A population of cells is contacted with an effective dose of a non-chelated biomacromolecule-reactive metal derivative, which selectively crosses the plasma membrane of non-viable cells, and which covalently modifies a biological macromolecule within the cell, for a period of time sufficient to permit entry into non-viable cells. The population of cells is then washed free of unbound viability reagent; and the presence of the metal within the cells is detected, wherein non-viable cells are selectively labeled with the viability reagent. | 2014-11-06 |
20140329273 | APPARATUS FOR COLLECTING DONATED STOOL - Disclosed are an apparatus for the collection of donated stool, a collection chamber for depositing (or collecting), processing and harvesting stool from a donor and a process for the preparation of a composition comprising donated stool and a pharmaceutically acceptable carrier. | 2014-11-06 |
20140329274 | METABOLIC FLUX MEASUREMENT, IMAGING AND MICROSCOPY - Provided herein are methods for measuring molecular flux rates of molecules of interest in a tissue sample in spatially-organized manner and generating output (e.g., an image, a heat map, a contour map, a table or a database) representing the molecular flux rates of each spatially-defined location of the sample. Provided herein are also the output, as well as systems and computer-readable medium with computer-executable instructions for determining molecular flux rates of molecules of interest in the sample. | 2014-11-06 |
20140329275 | BIOCATALYSIS CELLS AND METHODS - In one aspect, the invention provides a recombinant microbial cell modified to exhibit increased biosynthesis of a biosynthetic product compared to a wild-type control. In some cases, the biosynthetic product can be isocaproate; in other cases the biosynthetic product can be isovalerate. In another aspect, the invention provides methods of constructing such recombinant cells. In yet another aspect, the invention provides methods of using the cells to produce the biosynthetic product. In yet another aspect, the invention provides methods of harvesting organic acids from a fermentation culture. | 2014-11-06 |
20140329276 | METHODS FOR INCREASING N-GLYCAN OCCUPANCY AND REDUCING PRODUCTION OF HYBRID N-GLYCANS IN PICHIA PASTORIS STRAINS LACKING ALG3 EXPRESSION - Methods for increasing the yield and N-glycosylation site occupancy of paucimannose or complex N-glycans of recombinant glycoproteins produced in a recombinant host cell lacking dolichyl-P-Man:Man5GlcNAc2-PP-dolichyl alpha-1,3 mannosyltransferase (Alg3p) activity are disclosed. In particular, recombinant host cells are provided that comprise a disruption of the expression of an OS-9 family gene in the host cell. These recombinant host cells may then be used for producing recombinant glycoproteins. In further embodiments, the recombinant host cells further overexpress at least one heterologous single-subunit oligosaccharyltransferase, which in particular embodiments is capable of functionally suppressing the lethal phenotype of a mutation of at least one essential protein of the yeast oligosaccharyltransferase (OTase) complex. | 2014-11-06 |
20140329277 | METHOD FOR CULTURING CELLS IN ORDER TO PRODUCE SUBSTANCES - The invention concerns a method for culturing cells in order to produce substances. According to the invention a cell line producing substances is cultured while feeding a nutrient medium in such a manner that glucose limitation occurs in the culture solution. The degree of glucose limitation DGL=qGlc/qGlc | 2014-11-06 |
20140329278 | AMINO ACID SEQUENCES DIRECTED AGAINST IL-6R AND POLYPEPTIDES COMPRISING THE SAME FOR THE TREATMENT OF IL-6R RELATED DISEASES AND DISORDERS - The present invention relates to amino acid sequences that are directed against/and or that can specifically bind Interleukin-6 Receptor (IL-6R) with improved affinity and/or avidity, and/or that have an improved efficacy and/or potency, and which are capable of (partially, or preferably totally) blocking the IL-6/IL-6R interaction and/or inhibit signalization through IL-6, IL-6R and/or the IL-6/IL-6R complex. The invention further relates to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. | 2014-11-06 |
20140329279 | COMPOSITIONS FOR CELL CULTURE AND METHODS OF USING THE SAME - Supplementation of the bioflavonoids such as epigallocatechin gallate, rutin, naringin, or genistein into mammalian cell culture media are shown to be effective in reduction of acidic species variants on recombinant antibodies. The demonstrated reduction in acidic species through the use of bioflavonoids, facilitates the manufacturing of a less heterogeneous product with potential improvements in antibody structure and function. | 2014-11-06 |
20140329280 | PROCESSING BIOMASS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy or sugary materials, to produce ethanol and/or butanol, e.g., by fermentation. | 2014-11-06 |
20140329281 | Recombinant Production of Steviol Glycosides - Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express recombinant genes encoding UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol glycosides, e.g., Rebaudioside A and/or Rebaudioside D, which can be used as natural sweeteners in food products and dietary supplements. | 2014-11-06 |
20140329282 | CLOSED NUCLEIC ACID STRUCTURES - The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications. | 2014-11-06 |
20140329283 | ENZYMES - The present invention relates to new enzymes with improved properties and to compositions comprising these enzymes suitable for use in the production of a food, feed, or malt beverage product, such as in a brewing process. | 2014-11-06 |
20140329284 | Chimeric Polypeptides Having Beta-Glucosidase Activity and Polynucleotides Encoding Same - The present invention relates to chimeric polypeptides having beta-glucosidase activity. The present invention also relates to polynucleotides encoding the chimeric polypeptides; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the chimeric polypeptides | 2014-11-06 |
20140329285 | ON-FARM INTEGRATED HIGH-SOLIDS PROCESSING SYSTEM FOR BIOMASS - A method for on-farm processing a biomass feedstock into a useful industrial chemicals includes the steps of (a) delignifying the biomass feedstock to produce a delignified biomass, (b) subjecting the delignified biomass to cellulase production, (c) subjecting the delignified biomass with attached cellulase to simultaneous cellulolytic and solventogenic reactions to produce useful industrial chemicals (d) collecting and separating the useful industrial chemical from the fermentation broth and (e) collecting the fermentation residues. | 2014-11-06 |
20140329286 | EQUOL CONCENTRATION INCREASING AGENT CONTAINING PSICOSE AS ACTIVE INGREDIENT - The present invention relates to a processed food composition containing, as an active ingredient, psicose, which is a saccharide having the function of stimulating a daidzein conversion into equol by an intestinal microorganism in the human body. | 2014-11-06 |
20140329287 | COMPOSITIONS AND METHODS FOR LIPID PRODUCTION - Described herein, inter alia, are compositions, oleagnious organisms, and methods useful for producing lipids, lipid precursors, and/or oleochemicals. | 2014-11-06 |
20140329288 | IN VIVO CONVERSION OF LIGHT ENERGY INTO HYDROGEN GAS - The present disclosure provides isolated phototrophic cells for producing hydrogen and methods for use thereof. The methods involve inducing endo-hydrogenase expression in the phototrophic cells through the use of a regulatable promoter and harvesting light energy for hydrogen production. In preferred embodiments the hydrogenase is a member of the Hyq family of endo-hydrogenases and the isolated phototrophic cell is a | 2014-11-06 |
20140329289 | ANTI-FH APTAMERS, METHOD FOR PRODUCING SAME, AND USES THEREOF - The invention relates to nucleic acid aptamers binding specifically to factor H, to a method for obtaining same, and to the uses thereof, in particular for the purposes of purifying factor H. | 2014-11-06 |
20140329290 | NOVEL BRANCHED POLYETHYLENE GLYCOL AND USE THEREOF - The present invention allows formation of covalent bonds in a bioactive compound having at least one amino group so as to efficiently provide the compound modified with PEG. The present invention thus provides a functionalized polyethylene glycol having a structure that allows reaction of two aldehyde groups with one amino group to form two covalent bonds. | 2014-11-06 |
20140329291 | D4 DESATURASES AND D5 ELONGASES - Disclosed are methods and compositions related to ONC-T18, D4-desaturases, D5 elongases, their isolation, characterization, production, identification, and use for fatty acid production, as well as organisms containing these compositions and organisms expressing them. | 2014-11-06 |
20140329292 | NOVEL FUNGAL ENZYMES - This invention relates to novel enzymes and novel methods for producing the same. More specifically this invention relates to a variety of fungal enzymes. Nucleic acid molecules encoding such enzymes, compositions, recombinant and genetically modified host cells, and methods of use are described. The invention also relates to a method to convert lignocellulosic biomass to fermentable sugars with enzymes that degrade the lignocellulosic material and novel combinations of enzymes, including those that provide a synergistic release of sugars from plant biomass. The invention also relates to a method to release cellular content by degradation of cell walls. The invention also relates to methods to use the novel enzymes and compositions of such enzymes in a variety of other processes, including washing of clothing, detergent processes, biorefining, deinking and biobleaching of paper and pulp, and treatment of waste streams. | 2014-11-06 |
20140329293 | Polypeptides Having Cellobiohydrolase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2014-11-06 |
20140329294 | CELL CULTURE MEDIUM FOR ADAMTS PROTEIN EXPRESSION - The present invention provides culture mediums that are useful for the expression of ADAMTS proteins, such as ADAMTS13. Methods for the expression and purification of ADAMTS proteins are also provided. In some embodiments, the mediums and methods of the invention are useful for the expression of ADAMTS proteins having high specific activities. Also provided are ADAMTS, e.g., ADAMTS13, protein compositions with high specific activities, which are expressed and purified according to the methods provided herein. | 2014-11-06 |
20140329295 | METHOD FOR FORMING AND EXTRACTING SOLID PELLETS COMPRISING OIL-CONTAINING MICROBES - A process including: (a) mixing a microbial biomass, comprising oil-containing microbes, and at least one grinding agent capable of absorbing oil, to provide a disrupted biomass mix; (b) blending at least one binding agent with said disrupted biomass mix to provide a fixable mix capable of forming a solid pellet; and, (c) forming the solid pellet from the fixable mix. The process optionally includes extracting the solid pellet with a solvent to provide an extracted microbial oil. | 2014-11-06 |
20140329296 | NOVEL EXTREME THERMOPHILIC BACTERIA OF THE GENUS CALDICELLULOSIRUPTOR - The present disclosure pertains to novel isolated cellulolytic extreme thermophilic bacterial cells belonging to the genus | 2014-11-06 |
20140329297 | Polymeric Thin-Film Tube Connectors, Bioreactors, Systems and Methods - Polymeric thin-film tube connectors, bioreactors, systems and methods are described, the connectors allowing connection of a polymeric thin-film tube of a bioreactor chamber with a complementary part, typically, rigid port of flange of the bioreactor or bioreactor system. Further, bioreactors having a thin-film reactor chamber and one or more connectors are described. The thin-film reactor chamber can have a thin-film wall for enclosing culture medium and microorganisms. The connector can include a flexible boot. An opening of the thin-film wall couples to the flexible boot. The thin-film wall can extend through a reactor end of the flexible boot and can rest against an interior surface of the flexible boot. The connectors are particularly advantageous for connecting standard utilities in a bioreactor system to thin-film bioreactor chambers or capsules having a thin-film tubular opening. | 2014-11-06 |
20140329298 | Solid Waste Digestion System - A system for digesting biodigestible feed that preferably includes the steps of comminuting the feed, introducing feed, an oxygen-containing gas, an accelerant, and bacteria into a digestion zone, the bacteria being suitable for digesting the feed under aerobic, anaerobic, and anoxic conditions. The contents of the digestion zone can be changed from aerobic operation to either anoxic or anaerobic operation, or vice versa, without changing the bacteria in the digestion zone. | 2014-11-06 |
20140329299 | METHOD AND PLANT FOR SEPARATING CARBON DIOXIDE FROM CRUDE GASES CONTAINING METHANE - A method for removing carbon dioxide from methane-containing crude gases, especially biogas, by pressure scrubbing, using a physical solvent, includes circulating the contaminated scrubbing solution after regeneration. A plant suitable for carrying out the method considerably reduces the methane loss which arises through methane slippage and improves the overall energy balance of the method. Laden scrubbing solution obtained from the pressure scrubbing is treated at least in one flash stage before the first stripping stage. In addition, a nitrogen, oxygen, carbon dioxide and methane-containing gas mixture drawn off from the flash stage is cleaned in a scrubbing stage by purified scrubbing solution drawn off from the second stripping stage and supplied in countercurrent, forming a high-purity methane gas. | 2014-11-06 |
20140329300 | DEVICE FOR ANALYZING A SAMPLE AND METHOD OF USING THE SAME - This disclosure is directed to a device and method of using the device to analyze a sample. The sample may be a suspension, a portion of the suspension, a particular component of the suspension, or the like. For example, the suspension may be blood, the portion may be buffy coat, and the component may be circulating tumor cells. The device may be used to hold the sample for imaging or further processing. The device comprises a cavity that may be sealed on one side by a porous membrane and on an opposite side by a non-porous cover. The porous membrane allows for reagents to be introduced to the sample without diluting the sample. The non-porous cover allows for imaging of the sample. The device further comprises a nozzle for introducing the sample into a space within the cavity. | 2014-11-06 |
20140329301 | MULTIPLEXED DIAGNOSTIC DETECTION APPARATUS AND METHODS - Disclosed herein are devices configured for the amplification and detection of multiple targets from a sample, and methods of using the same. The devices disclosed herein comprise microfluidic cartridges have a first stage (amplification) and a second (detection) stage. The two-stage design of the cartridges enables testing for multiple targets within a sample, i.e., from a single nucleic acid amplification reaction. Methods for the amplification and detection of a plurality of target nucleic acids from a sample are also disclosed herein. | 2014-11-06 |
20140329302 | WIRELESS BASED MARINE PATHOGENS DETECTION SYSTEM - Embodiments of the present invention provide antibody functionalized high electron mobility transistor (HEMT) devices for marine or freshwater pathogen sensing. In one embodiment, the marine pathogen can be | 2014-11-06 |
20140329303 | In-vitro contact lens testing - Contact lens testing apparatuses and method for testing contact lenses for analytes are presented. In an aspect, a device is provided that includes a housing configured to hold one or more contact lenses, and a testing compartment provided within the housing and comprising a reagent, the reagent configured to facilitate a chemical reaction in response to the existence of a predetermined biomarker disposed on or within a contact lens placed in the testing compartment, wherein the chemical reaction produces a known result related to state information of an individual from which the biomarker was generated. | 2014-11-06 |
20140329304 | RAPID PEPTIDOGLYCAN-BASED ASSAY FOR DETECTION OF BACTERIAL CONTAMINATION - The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-β-D-glucan in a sample. | 2014-11-06 |
20140329305 | DEVICES, METHODS, AND SYSTEMS FOR NUCLEIC ACID AMPLIFICATION - A microfluidic device comprising a first surface portion and a first sample retaining element, which have differing affinities to a fluid, and a method comprising supplying a sample to such a device. In some embodiments, the differing affinity is a result of plasma, ion embedding, surface charging, chemical, optical, electronic and/or electromagnetic treatment. Also, a microfluidic device comprising at least one microcapillary device having a sample retaining element, at least one surface of which exhibits hydrophobicity, hydrophilicity, electromagnetic force exertion and electrostatic force exertion. Also, a microfluidic device comprising a first element having a hydrophilic pattern comprising at least a first sample retaining element. Also, a method comprising supplying a sample to a channel between a first element and a second element, and inducing in the first element at least one hydrophilic pattern by electrets or by internal or external electrodes to provide a charged surface. | 2014-11-06 |
20140329306 | ANAEROBIC DIGESTER SERVICE DEVICE WITH A SEPARATION CURTAIN - A digester tank has a servicing device that allows access to the interior of the digester tank. The sealing device may have a movable retention wall adapted to resist the pressure of the gas inside the digester by one or more of a) increasing the bending resistance of the retention wall, for example by inflating pockets in the wall, b) forcing the lower edge of the retention wall downwards, for example by weight, a mechanical arm or inflating pockets, and c) providing a mechanical structure within the retention wall, for example a non-moving cage-like structure. A sealing device with a fixed wall has a pipe with an isolation valve between the headspace of the tank and the inside of the sealing device. In operation, a seal may be formed across the separation curtain by raising the level of the liquid contents and closing the isolation valve. | 2014-11-06 |
20140329307 | Method and Device for Conducting Biochemical or Chemical Reactions at Multiple Temperatures - Methods and devices for conducting chemical or biochemical reactions that require multiple reaction temperatures are described. The methods involve moving one or more reaction droplets or reaction volumes through various reaction zones having different temperatures on a microfluidics apparatus. The devices comprise a microfluidics apparatus comprising appropriate actuators capable of moving reaction droplets or reaction volumes through the various reaction zones. | 2014-11-06 |
20140329308 | PLATFORM FOR IMMOBILIZATION AND OBSERVATION OF SUBCELLULAR PROCESSES - A method of immobilizing matter for imaging that includes providing an array of nanofibers and directing matter to the array of the nanofibers. The matter is immobilized when contacting at least three nanofibers of the array of nanofibers simultaneously. Adjacent nanofibers in the array of nanofibers may be separated by a pitch as great as 100 microns. The immobilized matter on the array of nanofibers may then be imaged. In some examples, the matter may be cell matter, such as protoplasts. | 2014-11-06 |
20140329309 | Ribosomal Promoters for Production in Microorganisms - The present invention provides methods and compositions of improved expression systems in microorganisms. The methods and compositions comprise a ribosomal promoter derived from a | 2014-11-06 |
20140329310 | Activated T Cells - Provided are compositions comprising activated T cells and activated T cells armed with bispecific antibodies directed at cancer antigens which are poor responders to alloantigen, and methods of using the compositions to help engraftment and improve anti-tumor effects. | 2014-11-06 |
20140329311 | INDUCTION OF HUMAN EMBRYONIC STEM CELL DERIVED CARDIAC PACEMAKER OR CHAMBER-TYPE CARDIOMYOCYTES BY MANIPULATION OF NEUREGULIN SIGNALING - The present invention is directed to methods of producing cardiomyocytes having a nodal/pacemaker phenotype and cardiomyocytes having an atrial/ventricular phenotype. Isolated populations of nodal/pacemaker and atrial/ventricular cardiomyocytes are also disclosed. Methods of treating a subject having cardiac arrhythmia and a subject in need of cardiac tissue repair using the isolated populations of nodal/pacemaker cardiomyocytes and atrial/ventricular cardiomyocytes, respectively, are also disclosed. | 2014-11-06 |
20140329312 | METHOD FOR PRODUCING HUMAN MONOCLONAL IMMUNOGLOBULIN G ANTIBODIES - The present invention a method for producing a human immunoglobulin G (IgG) antibody using a prime-boost regime in a Bone Marrow Liver Thymic (BLT) mouse. | 2014-11-06 |
20140329313 | EXPRESSION IN INSECT CELLS OF GENES WITH OVERLAPPING OPEN READING FRAMES, METHODS AND COMPOSITIONS THEREFOR - The present teachings disclose nucleic acid cassettes for expressing in an insect cell a plurality of polypeptides encoded by a gene comprising overlapping open reading frames (ORFs). A cassette comprises, in 5′ to 3′ order, a) a first insect cell-operable promoter, b) a 5′ portion of a gene comprising a first ORF of the gene, c) an intron comprising a second insect cell-operable promoter, and d) a 3′ portion of the gene comprising at least one additional ORF. Vectors and insect cells comprising the cassettes are also disclosed, as well as methods for production of recombinant adeno-associated virus in insect cells using the cassettes. | 2014-11-06 |
20140329314 | ENRICHED POPULATIONS OF CARDIOMYOCYTE LINEAGE CELLS FROM PLURIPOTENT STEM CELLS - The invention provides methods for depleting extraneous phenotypes from a mixed population of cells comprising the in vitro differentiated progeny of primate pluripotent stem cells, The invention also provides cell populations enriched for target cell populations which are the differentiated in vitro progeny of primate pluripotent stem cells. | 2014-11-06 |
20140329315 | Methods for Producing Insulin-Secreting Beta Cells From Human Pluripotent Stem Cells - A method of culturing human pluripotent stem cells to produce pancreatic lineage, the method comprising the steps of (a) culturing the stem cells in the presence of a chemically defined medium comprising an effective amount of FGF, Activin A, and BMP; (b) culturing the cells from step (a) in the presence of a chemically defined medium comprising an effective amount of insulin, transferrin, and selenium (ITS), and FGF; (c) culturing the cells from step (b) in the presence of a chemically defined medium comprising an effective amount of insulin, transferrin, and selenium (ITS), and Noggin-Nicotinamide-Retinoic acid; and (d) culturing the cells from step (c) in the presence of a serum free chemically defined medium (ITSFINE and Noggin) comprising an effective amount of ITS, FGF7, islet neogenesis associated peptide (INGAP), nicotinamide, and Exendin-4, wherein pancreatic lineage cells are produced, wherein the pancreatic lineage cells are insulin | 2014-11-06 |
20140329316 | TISSUE REPAIR WITH MULTIPOTENT CELLS - The invention relates to the field of medical science, in particular to technology directed at repairing defects in living, preferably human, tissue. The present invention provides a method for inducing differentiation of multipotent cells to a desired cell type, as well as a method for repairing a tissue defect in a human or animal patient using the concept of said method for inducing differentiation of multipotent cells. The invention further relates to a kit for carrying out the method for repairing a tissue defect. | 2014-11-06 |
20140329317 | METHOD FOR CULTURING PLURIPOTENT STEM CELL - The present invention provides a method of maintaining and amplifying pluripotent stem cells, including repeating the following steps:
| 2014-11-06 |
20140329318 | ISOLATED HUMAN LUNG PROGENITOR CELLS AND USES THEREOF - Provided herein are methods and compositions relating, in part, to the generation of human progenitor cells committed to the lung lineage and uses of such cells for treatment of lung diseases/disorders or injury to the lung. Whether an adult stem cell can be isolated from human adult lung remains controversial in the art and at present, methods for isolating and using adult lung stem cells from humans lack reproducibility. Thus, the methods and compositions described herein are advantageous over the present state of knowledge in the art and permit the generation of human lung progenitor cells for treatment, tissue engineering, and screening assays. | 2014-11-06 |
20140329319 | APPARATUS AND METHOD FOR THE IMMUNOCAMOUFLAGE OF BIOLOGICAL CELLS - A device and method for polymer modification of biological cells includes pumping a first liquid mixture comprising biological cells from a first reservoir to a mixing chamber and a second liquid mixture comprising activated polymer from a second reservoir to the same mixing chamber, independently controlling an output volume of the first and second liquid mixtures pumped into the mixing chamber using at least one pump, mixing the first and second liquid mixtures in the mixing chamber to produce a final mixture comprising polymer-modified biological cells, and evacuating the final mixture comprising polymer-modified biological cells from the mixing chamber into an output reservoir. | 2014-11-06 |
20140329320 | Nanoantibodies, Binding Chlamydia Trachomatis Antigen, Method for Inhibition of Infection Induced by Chlamydia Trachomatis - A nanoantibody specifically binding surface antigen of | 2014-11-06 |
20140329321 | METHODS AND COMPOSITIONS FOR CULTURING ENDODERM PROGENITOR CELLS IN SUSPENSION - Provided herein are methods for the in vitro differentiation of induced pluripotent stem cells, which have been expanded and/or maintained under defined conditions, into endodermal precursor cells (EPCs) that are capable of producing mono-hormonal beta cells. | 2014-11-06 |
20140329322 | METHOD OF DIFFERENTIATING GLIOBLASTOMA CELLS - A method of increasing differentiation of a cell line of glioblastoma cells is disclosed. A platelet-rich plasma (PRP) composition is prepared. The glioblastoma cells are cultured in the PRP composition for a time period sufficient for differentiation of the glioblastoma cells. | 2014-11-06 |
20140329323 | THREE-DIMENSIONAL NANOSTRUCTURED HYBRID SCAFFOLD AND MANUFACTURE THEREOF - A method of making a three-dimensional biocompatible scaffold capable of supporting cell activities such as growth and differentiation, the method includes providing a supporting grid that forms an open network and provides mechanical support of a second biocompatible material. The second biocompatible material has interconnected cavities that allow nutrients, metabolites and soluble factors to diffuse throughout the scaffold. The scaffold design can be understood as a hierarchically organised structure. At the micron to submicron length scale a top/down manufacturing approach is used to make a structure that will constitute the frame into which a bottom/up processing approach is applied to form an open porous scaffold with specific nano sized features. The advantage of this hierarcially organised design is that benefits can be drawn independently from both the micron and the nano sized structures, promoting specific cell activities and providing sufficient mechanical compliance. | 2014-11-06 |
20140329324 | FUNCTIONAL UNLIMITED CULTURE MEDIUM HAVING A NATURAL BIO-COMPOSITE INDUCER - A culture medium with natural inducer for biocomposites. In a general aspect the present disclosure is an indefinite functional culture medium with a natural inducer for biocomposites comprising: i) biomass plant incorporating high copper content, vitamins and trace minerals; and ii) a natural inducer, that is vegetable vinegar. | 2014-11-06 |
20140329325 | OPTOFLUIDIC MICRODEVICE FOR IN-VITRO LASER SURGERY AND TRANSFECTION INVOLVING CELLS AND MICROORGANISMS - A device for use in laser optical transfection of biological targets including an optofluidic microdevice and a piece of optical glass. The optofluidic microdevice has a central vertical outlet and a microchannel network that includes a plurality of entrapping channels with narrowings. The microchannel network is fused with the optical glass. In one aspect the device is used with a petri dish having an optical window. In another aspect the device is used with a well plate having a plurality of wells and associated optical windows. In a third aspect the device is used with a barrier. Each of the aspects forms a peripheral space around the optofluidic microdevice capable of retaining a live culture of biological targets and material that is desired to be injected into those biological targets. Polymer tubing is inserted into the central vertical outlet which connects the device to an external pump. The external pump provides an inward suction force which draws the biological targets from the peripheral space into the microchannel network. The biological targets are then captured at the openings or within the narrowings in the entrapping channels of the microchannel network where they can be transfected by laser light emitting from a laser through the optical glass. | 2014-11-06 |
20140329326 | SYNTHETIC GENE CLUSTERS - Methods for making synthetic gene clusters are described. | 2014-11-06 |
20140329327 | UREA-BASED SYNTHETIC URINE AND METHOD OF MANUFACTURING SAME - A synthetic urine solution and method of its manufacture are disclosed. The solution includes water having a pH between about 3 and about 10. The solution further includes creatinine, a means for removing bacteria from the solution so as to control or eliminate sepsis of the urine solution, preferably through the use of a biocide, and a urea-based compound. The solution exhibits a specific gravity of from 1.005 g/cm | 2014-11-06 |
20140329328 | Transformer Hydrogen Indicator - A reliable, low cost device for determining when dangerous levels of hydrogen gas have been generated in a transformer is disclosed. The hydrogen indicator is defined by a module assembly that threads into either the headspace or into the oil-filled body of a transformer. The module has an open interior that contains a film that incorporates a hydrogen-sensitive chemochromic indicator. The indicator film is visible through a lens. When the film has been exposed to hydrogen, chemical changes in the chemochromic indicator cause the film to change color—the color change is immediately visible through the lens. | 2014-11-06 |
20140329329 | FLUID STOP FOR MEASURED SAMPLE CONTAINMENT - A test cartridge includes an input channel to receive a liquid sample to be tested. A sample chamber is coupled to the input channel to receive the sample. An air permeable membrane is coupled between the sample chamber and ambient to prevent passage of the sample past the membrane and stop movement of the sample in the sample chamber. | 2014-11-06 |
20140329330 | SOIL TESTING SYSTEMS AND METHODS THEREOF - A soil tester is disclosed, and comprises a housing and a sampling apparatus. The housing includes an interior sampling chamber configured to receive a sample container. The sampling apparatus comprises a photodetector and a light source. The photodetector is mounted along a portion of the interior sampling chamber and has a variable resistance determined by a property of incident light. The light source is mounted to the interior sampling chamber in a transmissive orientation relative to the photodetector so that a beam of light can be transmitted from the light source to the photodetector through the interior sampling chamber. A display on the housing is responsive to the photodetector. | 2014-11-06 |