| 44th week of 2014 patent applcation highlights part 50 |
| Patent application number | Title | Published |
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| 20140322723 | DIABETES DIAGNOSIS THROUGH THE DETECTION OF GLYCATED PROTEINS IN URINE - The invention involves methods, compositions, and kits for detecting glycated proteins in a sample (e.g., urine or other bodily fluid) from a subject. Also provided are methods, compositions, and kits for diagnosing or following a diabetic condition of the subject or screening for a diabetic condition in a population of subjects based upon the detection of the glycated protein(s) in the sample. | 2014-10-30 |
| 20140322724 | HOMOGENEOUS COMPETITIVE LATERAL FLOW ASSAY - A patient or animal side method and assay for eliminating the hook effect in the detection of a target analyte such as an acute phase protein in a bodily fluid in which the target analyte comprises a member of a specific binding pair comprising applying the sample to a solid phase carrier material, generating a signal in accordance with downstream movement of the labelled first or second members and the target analyte to bind with the complimentary immobilised first or second members, and detecting the presence of the target analyte in accordance with the signal generated at the complimentary immobilised first or second members. | 2014-10-30 |
| 20140322725 | SENSORS AND ASSAYS FOR UBIQUITIN OR UBIQUITIN-LIKE PROTEINS - The present invention provides compositions comprising chimeric polypeptides that bind to free ubiquitin proteins or free ubiquitin-like proteins with high affinity, as well as chimeric polypeptides that bind to both free and conjugated ubiquitin proteins or free and conjugated ubiquitin-like proteins, and methods of using the chimeric polypeptides to determine the amount of free or total ubiquitin or free or total ubiquitin-like proteins in various types of samples. | 2014-10-30 |
| 20140322726 | MONOCLONAL ANTIBODY SPECIFICALLY RECOGNIZING ASPARAGINE SYNTHETASE - It is an object of the present invention to provide a monoclonal antibody, which is suitable for the quantitative analysis of asparagine synthetase in a cell. The present invention provides a monoclonal antibody which specifically recognizes asparagine synthetase that is present in a cell. | 2014-10-30 |
| 20140322727 | ASSAY - The present invention discloses an assay device ( | 2014-10-30 |
| 20140322728 | Compositions and Methods for Assessing Gastrointestinal Health - The present invention relates to kits designed for the collection of stool samples and methods of analyzing those samples for biological markers of maldigestion, inflammation, and imbalanced gut flora. | 2014-10-30 |
| 20140322729 | SERS, FLUORESCENCE, ABSORPTION, AND LUMINESCENCE DETECTION WITH FLOW-THROUGH MULTI-HOLE CAPILLARIES - 3-dimensional surface-enhanced Raman scattering (SERS), as well as absorption/fluorescence/luminescence detection is carried out using a platform based on nanoparticle-functionalized flow-through multi-hole capillaries for rapid analyte detection. The configuration provides an increased active area and fluidic channels for efficient sample delivery, and also confines and transmits light for a large signal accumulation. Using a capillary consisting of thousands of micron-sized holes adsorbed with gold nanoparticles, a detection limit better than 100 fM is achieved. | 2014-10-30 |
| 20140322730 | Botulinum Neurotoxin E Receptors and Uses Thereof - An isolated polypeptide comprising an amino acid sequence selected from amino acids 506-582 of SV2A, wherein position 573 is N and is glycosylated, or amino acids 449-525 of SV2B, wherein position 516 is N and is glycosylated. The present invention also provides an antibody that binds specifically to the polypeptide, an isolated nucleic acid comprising a polynucleotide that encodes the polypeptide; a method for reducing BoNT/E toxicity in an animal; a method for identifying an agent that blocks or inhibits binding between BoNT/E and an SV2A or SV2B protein; a method for monitoring synaptic vesicle endo- or exocytosis, a method for specifically delivering a chemical entity to a cell which has a specific receptor to a BoNT toxin. Also provided are a chimeric toxin for targeting a proteolytic domain of a toxin to a cell, the chimeric toxin comprising a catalytic or proteolytic domain of the BoNT toxin, and a ligand or a fragment thereof for a non-BoNT receptor on the cell; a method for targeting a proteolytic domain of a BoNT toxin to a cell, an isolated non-neuronal cell comprising a BoNT toxin receptor; and a method for screening for an inhibitor of a BoNT toxin. | 2014-10-30 |
| 20140322731 | AMYLOID BETA-DERIVED DIFFUSIBLE LIGANDS (ADDLs), ADDL-BINDING MOLECULES, AND USES THEREOF - The invention herein comprises amyloid beta-derived diffusible ligands (ADDLs), compositions comprising ADDLs, ADDL-surrogates, ADDL-binding molecules, and methods of using any of the foregoing compounds and compositions. ADDLs comprise amyloid β protein assembled into soluble, globular, non-fibrillar, oligomeric structures capable of activating specific cellular processes. The invention also comprises methods of generating ADDL-specific antibodies and methods of using ADDL-specific antibodies for assaying the formation, presence, receptor protein binding and cellular activity of ADDLs, as well as using such antibodies to detect compounds that block the formation or activity of ADDLs, and methods of identifying such compounds. The invention further provides methods of using ADDL-specific antibodies in modulating ADDL formation and/or activity, inter alia in the treatment of learning and/or memory disorders. | 2014-10-30 |
| 20140322732 | METHOD FOR BIOMARKER AND DRUG-TARGET DISCOVERY FOR PROSTATE CANCER DIAGNOSIS AND TREATMENT AS WELL AS BIOMARKER ASSAYS DETERMINED THEREWITH - The invention relates to a method for the determination of a cancer diagnostic/therapeutic biomarker assay and drug-targets including the following steps: (a) identification of potential candidate protein/peptide biomarkers and drug-targets based on the measurement of protein/peptide constituent concentrations in tissue sample proteomes as well as serum, plasma or any other derivatives of blood, or blood itself sample proteomes derived from healthy non-human mammalian individuals as well as from cancerous non-human mammalian individuals and qualitatively selecting as potential candidate protein/peptide biomarkers those which show a pronounced differential behaviour between healthy and cancerous sample proteomes; (b) optional verification of the potential candidate protein/peptide biomarkers as identified in step (a) by quantitative mass spectrometric measurement of the potential candidate protein biomarkers in serum, plasma or any other derivatives of blood, or blood itself sample proteomes derived from healthy non-human mammalian individuals as well as from cancerous non-human mammalian individuals and selecting as candidate protein/peptide biomarkers those which show a mass-spectrometrically measurable quantitative differential behaviour between healthy and cancerous sample proteomes; (c) validation of the candidate protein/peptide biomarkers as identified in step (a), or as optionally verified in step (b), by mass spectrometric measurement and/or antibody-based assays such as an Enzyme-Linked Immunosorbent Assay (ELISA) determination of the candidate protein biomarkers in serum, plasma or any other derivatives of blood, or blood itself sample proteomes derived from healthy human individuals as well as from cancerous human individuals and selecting as protein/peptide biomarkers those which show a mass-spectrometrically measurable and/or antibody-based assay detectable differential behaviour between healthy and cancerous sample proteomes; (d) application of statistical methods to uncover single or groups of protein/peptide biomarkers as validated in step (c) as signatures for the detection of patients with cancer. The invention furthermore relates to specific biomarker assays for the highly reliable diagnosis of cancer, specifically of localized or non-localized prostate cancer, using human serum, plasma or any other derivatives of blood, or blood itself. | 2014-10-30 |
| 20140322733 | OPTICAL METHOD AND DEVICE FOR IDENTIFYING AND QUANTIFYING ANALYTES - A method for analysing a metalloprotein and/or the interaction with its environment comprising the following steps: (a) Providing a medium that enhances the detection of the electromagnetic cross-section signal of metalloproteins, (b) Incorporating a metalloprotein to analyse into said medium, (c) Contacting said medium with electromagnetic radiation, (d) Obtaining the electromagnetic cross-section spectrum of said metalloprotein, (e) Determining from said electromagnetic cross-section spectrum at least one parameter related to one or several analytes of interest. | 2014-10-30 |
| 20140322734 | BIOMARKERS FOR PREDICTION, DIAGNOSIS, AND MONITORING OF PARKINSON'S DISEASE - A method for the risk detection, early diagnosis, prognosis, and monitoring of Parkinson's disease in an individual by measuring the amount of specific biomarkers present in a bodily fluid and comparing them to a reference level of biomarkers in a sample from a healthy person, a person previously diagnosed with Parkinson's disease, or an earlier sample from the individual of interest. | 2014-10-30 |
| 20140322735 | Method to Measure Endogenous Enzymatic Serum/Plasma Cholesterol Esterification by LCAT (Lecithin:Cholesterol Acyltransferase) Assay - The present invention provides a method for the assessment of cholesterol esterification through physiologically relevant pathways and incorporates the function of individual subject's endogenous HDL particles. This ex vivo approach avoids the use of an artificial substrate and provides for determination of LCAT activity that includes both the contribution of a subject's endogenous HDL function and endogenous LCAT protein, and is therefore a significant improvement to the biologic relevance. | 2014-10-30 |
| 20140322736 | METHOD FOR THE PRODUCTION OF HUMAN THROMBIN AND USES THEREOF - The present invention disclosed the cloning and the purification of a mutant of prethrombin-2, which contains a thrombin cleavage site instead of a factor Xa cleavage site. The stable mutant prethrombin-2 is able to convert itself autocatalytically into active α-thrombin in the absence of ecarin or factor Xa. The new concept of signal amplification using self-replicating enzymes can be applied to improve sensitivity of α-thrombin assays and also for the preparation of different enzymes. | 2014-10-30 |
| 20140322737 | FAST REACTION KINETICS OF ENZYMES HAVING LOW ACTIVITY IN DRY CHEMISTRY LAYERS - The present invention concerns a method for determining an analyte as well as a diagnostic element suitable therefore. In one particular form, a method for determining an analyte includes contacting a sample containing the analyte with a diagnostic element comprising a dry reagent layer. The dry reagent layer contains a mutated dehydrogenase which is specific for the analyte and an artificial coenzyme. The method also includes determining at least one of analyte presence and an amount of the analyte. | 2014-10-30 |
| 20140322738 | SUBSTRATES FOR COVALENT TETHERING OF PROTEINS TO FUNCTIONAL GROUPS OR SOLID SURFACES - The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates. | 2014-10-30 |
| 20140322739 | METHODS AND COMPOUNDS FOR DETECTING BETA-LACTAMASE ACTIVITY - The present invention relates to compounds for and a method of detecting beta-lactamase activity in a sample. The sample is contacted with a nanoparticulate tag. The nanoparticulate tag comprises a metal or a combination of metals, or it comprises a nanotube of a metal, boron nitride and/or carbon. The respective metal is capable of forming one of a covalent bond, a coordinative bond and a non-covalent interaction with a thio or a seleno group. The sample is contacted with a compound of one of general formulas (I)-(III) and (VII)-(IX). At least one beta-lactam moiety of the compound is cleaved by the beta-lactamase activity in the sample. As a result a cleavage moiety Z-A-Z, Z-A-Z—R | 2014-10-30 |
| 20140322740 | PSA PEPTIDASE ACTIVITY (PPA) AS A PROSTATE CANCER BIOMARKER - The present invention is directed to the diagnosis of cancer associated with enzymatically active PSA in samples. | 2014-10-30 |
| 20140322741 | Cell Free CD4 Quantitation and Methods of Use - The present invention provides a low-cost cell-free assay, the α-test, that provides point-of-care CD4 enumeration using a single platform assay thereby eliminating the need for high-end instrumentation, calibrated pipetting, and specialized technical training. The number of CD4 T cells in blood is driven by the concentration of the protein α1 proteinase inhibitor (α1PI, α1 antitrypsin, serpin A1). The invention features, in part, methods for determining the number of CD4+ T cells in a sample comprising determining the concentration of alpha 1 proteinase inhibitor (α1PI) in a sample; wherein the number of CD4+ T-cells is related to the concentration of α1PI. | 2014-10-30 |
| 20140322742 | METHODS AND A DEVICE FOR THE FORMATION OF THREE-DIMENSIONAL MULTICELLULAR ASSEMBLIES - The present invention relates to devices and associated methods for forming three-dimensional multicellular assemblies in vitro. Specifically, the present invention relates to devices comprising at least one three-dimensional multicellular assembly immobilised on a two-dimensional adhesive pattern, wherein said three-dimensional multicellular assembly has an organised structure with a normalised polarity and methods for the formation of three-dimensional multicellular assemblies having an organised structure. | 2014-10-30 |
| 20140322743 | POLYMER MICROFILTRATION DEVICES, METHODS OF MANUFACTURING THE SAME AND THE USES OF THE MICROFILTRATION DEVICES - A microfilter comprising a polymer layer formed from epoxy-based photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A microfilter comprising two or more polymer layers formed from epoxy-based photo-definable dry film, and a plurality of apertures or open areas each extending through the polymer layer. A method of forming a microfilter is also disclosed. The method includes providing a first layer of epoxy-based photo-definable dry film disposed on a substrate, exposing the first layer to energy through a mask to form a pattern, defined by the mask, in the first layer of dry film, forming, from the exposed first layer of dry film, a polymer layer having a plurality of apertures extending therethrough, the plurality of apertures having a distribution defined by the pattern, and removing the polymer layer from the substrate. Unique filter holder designs and methods appropriate to hold microfilters to collect the rare cells and allow performing assays in the filter holder are provided. The invention also describes the use of the microfilter and filter holder to collect rare cells from body fluids and perform assays. Rare cells collected on the microfilter in accordance with embodiments of the present invention can be used for medical and biological research applications. | 2014-10-30 |
| 20140322744 | CLONABLE TAG FOR CORRELATIVE LIGHT AND ELECTRON MICROSCOPY LABELING - The present invention relates to a new clonable label for Correlative Light and Electron Microscopy based on the combination of modified metallothionein and lanthanides. | 2014-10-30 |
| 20140322745 | OPTICAL METHOD FOR CONTROLLING THE MOVEMENT OF A SAMPLING TOOL - The present invention relates to a method of sampling biological material on a reference surface, such as the surface of an agar medium, with the aid of a sampling tool, said method making it possible to steer the displacement of the sampling tool towards the reference surface, from a first position towards a second position, and/or of the reference surface towards the sampling tool, from a first position towards a second position, said second position being a contact position in which the sampling tool and the reference surface are in contact, said method comprising the following steps:
| 2014-10-30 |
| 20140322746 | Multi-Way Sorter System and Method - Provided herein are improved multi-way cell sorter systems and methods. For example, provided are systems and methods for the collection of cells that are sorted in multiple directions. The systems and methods allow for the construction of a multi-way sorter (e.g., a ten-way sorter in the space that currently only allows four-way sorting). In addition the device may actively sense the arrival of drops (with cells of interest) at a sample tube, and trigger an alarm when the drops' arrivals deviate from an expected pattern. | 2014-10-30 |
| 20140322747 | OBJECT SELECTING DEVICE AND OBJECT SELECTING METHOD - An object selecting device includes a container configured to store liquid, a plate configured to support a selection object by being immersed into the liquid stored in the container, and a holder configured to hold the plate in a state where a bottom surface of the plate and an inner bottom part of the container are separated. The plate includes a through hole at a support position. The through hole includes a tapered portion configured to allow the selection object to precipitate along a direction of gravity and support the selection object in contact with the inner wall surface of the through hole. An opening area at the upper end of the tapered portion is larger than that at the lower end of the tapered portion. Only the selection object can be supported out of the collection of objects having different shapes. | 2014-10-30 |
| 20140322748 | System and Method to Improve Yield of Sorted Particles - Provided herein are systems and methods for improving yield of sorted particles. In one embodiment, for example, there is provided a system including: (a) a flow cytometer to analyze a sample, wherein the flow cytometer provides a parameter plot based on the analysis of the sample; (b) a user-interface, wherein a user can define a coincidence acceptance gate in the parameter plot, and wherein the coincidence acceptance gate identities a non-target particle population in the sample that may be accepted with a target particle in a subsequent sort analysis; and (c) a sort analysis system to sort particles within the sample, while accepting particles defined by coincidence acceptance gate. | 2014-10-30 |
| 20140322749 | Method for Measuring Color Change of Oxidation-Reduction Indicator - Provided is a method for measuring a color change of an oxidation-reduction indicator, which method is applicable to measurement of the cariogenic bacterial count and the like. A color change of an oxidation-reduction indicator is measured by a method for measuring a color change of an oxidation-reduction indicator, the method comprising reacting a test reagent with a test sample and measuring a color change, wherein the test reagent contains an oxidation-reduction indicator, an oxidation-reduction promoter, and a halogen salt. | 2014-10-30 |
| 20140322750 | BLOOD ANALYZER, BLOOD ANALYSIS METHOD AND HEMOLYTIC AGENT - This blood analyzer includes a sample preparation portion preparing a measurement sample free from a labeling substance from a blood sample and a hemolytic agent free from a labeling substance, a light information generation portion generating fluorescent information and at least two types of scattered light information from the measurement sample and a control portion performing a first classification of white blood cells in the measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of the fluorescent information and the two types of scattered light information. | 2014-10-30 |
| 20140322751 | MUTANT PYRROLYSYL -tRNA SYNTHETASE, AND METHOD FOR PRODUCTION OF PROTEIN HAVING NON-NATURAL AMINO ACID INTEGRATED THEREIN BY USING THE SAME - Method for incorporating a lysine derivative (particularly an N | 2014-10-30 |
| 20140322752 | REFOLDING TRANSFORMING GROWTH FACTOR BETA FAMILY PROTEINS - Compositions and methods for folding proteins belonging to the transforming growth factor beta superfamily are disclosed. The compositions and methods allow for the folding of such proteins when produced in an expression system that does not yield a properly folded, biologically active product. | 2014-10-30 |
| 20140322753 | Polypeptides Having Endoglucanase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 2014-10-30 |
| 20140322754 | METHOD FOR HIGHLY EXPRESSING RECOMBINANT PROTEIN OF ENGINEERING BACTERIA AND USE THEREOF - Provided are methods for highly expressing recombinant protein of engineering bacteria and the use thereof. The method comprises the following steps: (1) engineering bacteria of | 2014-10-30 |
| 20140322755 | CHEMICALLY MODIFIED SOPHOROLIPIDS AND USES THEREOF - The present disclosure provides a sophorolipid composition that can be used for inducing protein expression in a fermentation host. The sophorolipid composition described herein can be prepared from a natural sophorolipid mixture. Acid treatment of the natural sophorolipid mixture results in a mixture of monoacetylated, deacetylated, and/or diacetylated sophorolipids. The chemically modified sophorolipid composition, or isolated components of the chemically modified sophorolipid composition, can be used as inducers for protein production in filamentous fungi. | 2014-10-30 |
| 20140322756 | METHOD FOR MAKING MULTISPECIFIC ANTIBODIES HAVING HETEROMULTIMERIC AND COMMON COMPONENTS - The invention relates to a method of preparing heteromultimeric polypeptides such as bispecific antibodies, bispecific immunoadhesins and antibody-immunoadhesin chimeras. The invention also relates to the heteromultimers prepared using the method. Generally, the method provides a multispecific antibody having a common light chain associated with each heteromeric polypeptide having an antibody binding domain. Additionally the method further involves introducing into the multispecific antibody a specific and complementary interaction at the interface of a first polypeptide and the interface of a second polypeptide, so as to promote heteromultimer formation and hinder homomultimer formation; and/or a free thiol-containing residue at the interface of a first polypeptide and a corresponding free thiol-containing residue in the interface of a second polypeptide, such that a non-naturally occurring disulfide bond is formed between the first and second polypeptide. The method allows for the enhanced formation of the desired heteromultimer relative to undesired heteromultimers and homomultimers. | 2014-10-30 |
| 20140322757 | ANTIBODIES THAT BIND COLONY STIMULATING FACTOR 1 RECEPTOR (CSF1R) - Antibodies that bind CSF1R are provided. Antibody heavy chains and light chains that are capable of forming antibodies that bind CSF1R are also provided. Polynucleotides encoding antibodies to CSF1R are provided. Polynucleotides encoding antibody heavy chains and lights chains are also provided. Methods of treatment using antibodies to CSF1R are provided. Such methods include, but are not limited to, methods of treating rheumatoid arthritis, bone loss, and multiple sclerosis. | 2014-10-30 |
| 20140322758 | ADDITION OF IRON TO IMPROVE CELL CULTURE - The present invention provides, among other things methods of increasing cell density, viability and/or titer in a cell culture including steps of adding a composition comprising iron to the cell culture. | 2014-10-30 |
| 20140322759 | PHI29 DNA POLYMERASE MUTANTS HAVING INCREASED THERMOSTABILITY AND PROCESSIVITY - Mutants of bacteriophage phi29 DNA polymerase with increased protein stability and increased half-life, compared to wild type DNA polymerase. The disclosed mutants are more stable in reaction mixtures with or without DNA. The inventive phi29 DNA polymerase mutants generate more amplification product. The inventive phi29 DNA polymerase mutants amplify genomic DNA with less bias compared to wild type DNA polymerase. Selected mutations increase the affinity of polymerase for DNA template. | 2014-10-30 |
| 20140322760 | METHOD FOR PRODUCING VIRUS VECTOR FOR GENE TRANSFER - The present invention discloses a cell system, as a host cell to be infected with an F gene-deficient virus, which can constitutively and stably express the F protein, and a method for producing an F gene-deficient virus by utilizing the cell. A non-proliferative human parainfluenza type 2 virus vector is produced by co-culturing an F gene-deficient human parainfluenza type 2 virus with a Vero cell having the F gene of human parainfluenza type 2 virus in such a manner that the F gene is non-inducibly expressed, and isolating viral particles from a culture supernatant. | 2014-10-30 |
| 20140322761 | METHOD OF PREPARING SAMPLE FOR NUCLEIC ACID AMPLIFICATION REACTION, NUCLEIC ACID AMPLIFICATION METHOD, AND REAGENT AND MICROCHIP FOR SOLID PHASE NUCLEIC ACID AMPLIFICATION REACTION - Provided is a method of preparing a sample for nucleic acid amplification reaction, including: a procedure of dissolving a solid phase reagent at least containing DNA polymerase, cyclodextrin, and a binder, in a liquid containing a nucleic acid. | 2014-10-30 |
| 20140322762 | Treatment of Cellulosic Material and Enzymes Useful Therein - The present invention relates to the production of sugar hydrolysates from cellulosic material. The method may be used e.g. for producing fermentable sugars for the production of bioethanol from lignocellulosic material. Cellulolytic enzymes and their production by recombinant technology is described, as well as uses of the enzymes and enzyme preparations. | 2014-10-30 |
| 20140322763 | C1-C2 Organic Acid Treatment of Lignocellulosic Biomass to Produce Acylated Cellulose Pulp, Hemicellulose, Lignin and Sugars and Fermentation of the Sugars - A process for production of C5 and C6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a C | 2014-10-30 |
| 20140322764 | MUTANT XYLANASE, MANUFACTURING METHOD AND USE THEREFOR, AND METHOD FOR MANUFACTURING SACCHARIFIED LIGNOCELLULOSE - What is aimed at is provision of an inexpensive and efficient saccharification method for lignocellulose using a thermostable xylanase and provision of a mutant xylanase that has a substitute amino acid residue, and that exhibits stable activity even under severe conditions in which enzymes easily inactivate, and that provides an initial rate of reaction not significantly reduced as compared to a wild-type xylanase corresponding to the mutant xylanase. Provided is a method of producing a saccharified product of lignocellulose, including contacting a lignocellulosic raw material with a thermostable xylanase, and a mutant xylanase that provides an initial rate of reaction that is at least 70% of that provided by a wild-type xylanase corresponding thereto, that has a xylanase activity after heat treatment at 50° C. for 24 hours that is at least 50% of its xylanase activity before the heat treatment, and that has a substitute amino acid residue. | 2014-10-30 |
| 20140322765 | Enhanced Processive Cellulases - Nucleic acid sequences encoding chimeric polypeptides that exhibit enhanced cellulase activities are disclosed herein. These nucleic acids may be expressed in hosts such as fungi, which in turn may be cultured to produce chimeric polypeptides. Also disclosed are chimeric polypeptides and their use in the degradation of cellulosic materials. | 2014-10-30 |
| 20140322766 | C1-C2 Organic Acid Treatment of Lignocellulosic Biomass to Produce Acylated Cellulose Pulp, Hemicellulose, Lignin and Sugars and Fermentation of the Sugars - A process for production of C5 and C6 sugar enriched syrups from lignocellulosic biomass and fermentation products therefrom is described. A lignocellulosic biomass is treated with a C | 2014-10-30 |
| 20140322767 | DROPLET MICROREACTOR - The present invention relates to a droplet microreactor, i.e. a microreactor consisting of a droplet of a specific liquid, the microreactor being wall-less, wherein the interface of the specific liquid with the ambient environment and with the support on which the droplet is deposited defines the limits of the microreactor. The microreactor is characterized in that it consists of a droplet comprising at least one ionic liquid. The present invention also relates to methods for carrying out chemical or biochemical reactions and/or mixes using said droplet microreactor, and also to a lab-on-chip comprising a microreactor according to the invention. | 2014-10-30 |
| 20140322768 | Polypeptides Having Peroxygenase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2014-10-30 |
| 20140322769 | BIOCATALYSTS FOR THE PREPARATION OF HYDROXY SUBSTITUTED CARBAMATES - The present disclosure relates to engineered ketoreductase polypeptides for the preparation of hydroxyl substituted carbamate compounds, and polynucleotides, vectors, host cells, and methods of making and using the ketoreductase polypeptides. | 2014-10-30 |
| 20140322770 | Polypeptides Having Peroxygenase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having peroxygenaseactivity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2014-10-30 |
| 20140322771 | Lipid Production - The invention relates to methods for producing lipids and in particular methods for producing glycerolipids suitable for use in generating biofuels. The glycerolipids are produced through the modification of a cell so as to increase phosphatidic acid via the inhibition of the phosphatidylethanolamine N-methyltransferase (PEMT) pathway. The invention further relates to genetically modified plants and microorganisms in which the production of glycerolipids/oil is increased. | 2014-10-30 |
| 20140322772 | PRODUCTION OF CARBOXYLATES AND METHANE FROM BIOMASS WASTE - Methods and systems for producing and removing C | 2014-10-30 |
| 20140322773 | METHOD FOR PRODUCING LACTIC ACID - Provided is a method for producing lactic acid which does not require neutralization and crude purification associated therewith both of which give a high load to the environment. | 2014-10-30 |
| 20140322774 | MICROBIAL PRODUCTION OF N-BUTYRALDEHYDE - Microorganisms and methods of producing n-butyraldehyde with enhanced yields are presented in which a microorganism is engineered to enhance the conversion of a carbon source into n-butyraldehyde. The n-butyraldehyde is recovered by way of a gas stripping process that occurs during the conversion process, providing significantly greater product yield than post-fermentation recovery of n-butyraldehyde alone. | 2014-10-30 |
| 20140322775 | METHOD FOR PRODUCING SCYLLO-INOSITOL - It is intended to provide a novel NAD | 2014-10-30 |
| 20140322776 | OVEREXPRESSION OF GENES THAT IMPROVE FERMENTATION IN YEAST USING CELLULOSIC SUBSTRATES - The invention relates to recombinant yeast host cells that overexpress proteins to improve glucose utilization, pentose sugar utilization and/or production of a fermentation product in a fermentation reaction. | 2014-10-30 |
| 20140322777 | PROCESS OF SEPARATING COMPONENTS OF A FERMENTATION BROTH - A process of isolating 1,4-butanediol (1,4-BDO) from a fermentation broth includes separating a liquid fraction enriched in 1,4-BDO from a solid fraction comprising cells, removing water from said liquid fraction, removing salts from said liquid fraction, and purifying 1,4-BDO. A process for producing 1,4-BDO includes culturing a 1,4-BDO-producing microorganism in a fermentor for a sufficient period of time to produce 1,4-BDO. The 1,4-BDO-producing microorganism includes a microorganism having a 1,4-BDO pathway having one or more exogenous genes encoding a 1,4-BDO pathway enzyme and/or one or more gene disruptions. The process for producing 1,4-BDO further includes isolating 1,4-BDO. | 2014-10-30 |
| 20140322778 | PRODUCTION OF ISOPROPANOL BY IMPROVED RECOMBINANT STRAINS - The expression vector includes: the nucleic acids coding for the polypeptides forming a polypeptide complex having an enzyme activity allowing acetoacetyl-CoA to be converted to acetoacetate; optionally, at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetoacetate to be converted to acetone; and at least one nucleic acid coding for a polypeptide having an enzyme activity allowing acetone to be converted to isopropanol; the expression of the nucleic acids being controlled by a single constitutive promoter located upstream of the abovementioned nucleic acids. | 2014-10-30 |
| 20140322779 | EUKARYOTIC ORGANISMS AND METHODS FOR INCREASING THE AVAILABILITY OF CYTOSOLIC ACETYL-COA, AND FOR THE PRODUCING 1,3-BUTANEDIOL - Provided herein are non-naturally occurring eukaryotic organisms that can be engineered to produce and increase the availability of cytosolic acetyl-CoA. Also provided herein are non-naturally occurring eukaryotic organisms having a 1,3-butanediol (1,3-BDO) pathway, and methods of using such organisms to produce 1,3-BDO. | 2014-10-30 |
| 20140322780 | Methylbutanol as an Advanced Biofuel - This invention describes genes, metabolic pathways, microbial strains and methods to produce methylbutanol and other compounds of interest from renewable feedstocks. | 2014-10-30 |
| 20140322781 | CATALYTIC CONVERSION OF ALCOHOLS TO HYDROCARBONS WITH LOW BENZENE CONTENT - A method for converting an alcohol to a hydrocarbon fraction having a lowered benzene content, the method comprising: converting said alcohol to a hydrocarbon fraction by contacting said alcohol, under conditions suitable for converting said alcohol to said hydrocarbon fraction, with a metal-loaded zeolite catalyst catalytically active for converting said alcohol to said hydrocarbon fraction, and contacting said hydrocarbon fraction with a benzene alkylation catalyst, under conditions suitable for alkylating benzene, to form alkylated benzene product in said hydrocarbon fraction. Also described is a catalyst composition useful in the method, comprising a mixture of (i) a metal-loaded zeolite catalyst catalytically active for converting said alcohol to said hydrocarbon, and (ii) a benzene alkylation catalyst, in which (i) and (ii) may be in a mixed or separated state. A reactor for housing the catalyst and conducting the reaction is also described. | 2014-10-30 |
| 20140322782 | METHOD AND APPARATUS FOR BIO-FUEL SEEDING - A method and apparatus is provided for microbial seeding and amendment of traditional alternative fuels production systems and processes using immobilized microbe bioreactors. The system addition utilizes attachment of yeast or other microbial consortia to a substrate to enhance alternative fuels production in fermentation processes. The system allows for the maintenance of a constant concentrated microbial population, thus enhancing alternative fuels production by stabilizing microbial populations. Desired aerobic and anaerobic conditions are maintained using a microbubble aeration device coupled to the Immobilized Microbe Bioreactor (IMBR) seeding reactors. Generation of the microbial populations for seeding requires control of aerobic and anaerobic conditions to ensure growth of a microbial population acclimated to elevated alternative fuels concentrations. | 2014-10-30 |
| 20140322783 | Engineering an Increase in Ethanol Production by Altering Cofactor Specificity - The present invention provides for the manipulation of cofactor usage in a recombinant host cell to increase the formation of desirable products. In some embodiments, the invention provides for a recombinant microorganism comprising a mutation in one or more native enzymes such that their cofactor specificity is altered in such a way that overall cofactor usage in the cell is balanced for a specified pathway and there is an increase in a specific product formation within the cell. In some embodiments, endogenous enzymes are replaced by enzymes with an alternate cofactor specificity from a different species. | 2014-10-30 |
| 20140322784 | SYSTEMS AND METHODS FOR MAGNETIC GUIDANCE AND PATTERNING OF MATERIALS - Systems and methods generally useful in medicine, cellular biology, nanotechnology, and cell culturing are discussed. In particular, at least in some embodiments, systems and methods for magnetic guidance and patterning of cells and materials are discussed. Some specific applications of these systems and methods may include levitated culturing of cells away from a surface, making and manipulating patterns of levitated cells, and patterning culturing of cells on a surface. Specifically, a method of culturing cells is presented. The method may comprise providing a plurality of cells, providing a magnetic field, and levitating at least some of the plurality of cells in the magnetic field, wherein the plurality of cells comprise magnetic nanoparticles. The method may also comprise maintaining the levitation for a time sufficient to permit cell growth to form an assembly. | 2014-10-30 |
| 20140322785 | Continuous Flow Bioreactor for Magnetically Stabilized Three-Dimensional Tissue Culture - The invention provides methods for rapid, continuous generation of cells and cell products using magnetically stabilized three-dimensional tissue culture. The invention also pertains to a continuous flow self-regulating closed system bioreactor system for magnetically stabilized three-dimensional tissue culture. The methods described here do not use traditional solid scaffolding for cell culture. | 2014-10-30 |
| 20140322786 | POLYMER MODIFIED SUBSTRATES, THEIR PREPARATION AND USES THEREOF - Provided are polymer modified substrates which comprise a) a substrate, b) a binding layer covalently attached to the surface of the substrate and covering at least a part of this surface; and c) a polymer brush formed by a plurality of polymer chains, each of which is covalently attached at one of its terminals to the binding layer. Moreover, methods are provided, for the preparation of the polymer modified substrates by polymerizing vinyl group containing monomers, such as vinylphosphonates, on a binding layer provided on a substrate. | 2014-10-30 |
| 20140322787 | METHOD FOR MANUFACTURING IN VITRO VASCULARIZED TISSUE - The present invention relates to a method for manufacturing an in vitro vascularized tissue, which enables obtaining the in vitro vascularized tissue by inducing in vitro angiogenesis in a tissue using vascular cells, for use in in vitro research of diseases in vascularized tissues and developing a treatment. The method for manufacturing the in vitro vascularized tissue comprises the steps of: supplying a hydrogel to cover the vascularized tissue cells; molding the same to form a supply hole, which is separated from the vascularized tissue cells, on the upper side of the hydrogel; and supplying vascular cells to the supply hole. | 2014-10-30 |
| 20140322788 | method for increasing protein thermal stability - The invention provides a simple and effective method for increasing thermal stability of a wide range of proteins, comprising fusing a self-assembling amphipathic peptide to the C- or N-terminal of target proteins. The fusion protein can have a half life up to 26 times longer than that of the wild type protein. | 2014-10-30 |
| 20140322789 | LINKER-BRIDGED GENE OR DOMAIN FUSION REVERSE TRANSCRIPTASE ENZYME - The present invention relates to combinations of a linker bridged gene or domain fusion reverse transcriptase enzyme, and more particularly, combinations of a linker bridged gene or domain fusion reverse transcriptase enzyme and their fusion construction utilizing for more efficient and quality DNA synthesis in reverse transcription. The composition of the invention includes a polymerase domain; a linker, consisting of 3-40 amino acids; and an RNase H domain, wherein the RNase H domain is either unmodified or modified with point mutations. The composition may further include another mutated RNase H, a mutated RNase A, and an additional linker which consists of 3-40 amino acids. | 2014-10-30 |
| 20140322790 | METHODS, PEPTIDES AND BIOSENSORS USEFUL FOR DETECTING A BROAD SPECTRUM OF BACTERIA - Described herein are methods of detecting a wound infection and for detecting the presence or absence of bacteria, for example, wound bacteria in a sample, by contacting a sample with a peptide substrate derived from the modification of the reactive site loop (RSL) domain of the α1-proteinase inhibitor. In the current invention, we have demonstrated that these peptide substrates without the alpha 1 protein can be efficiently used as peptide substrates. The modification or the absence of modification of this peptide substrate by the enzyme produced and/or secreted by the bacteria, can serve as an indicator for the presence or absence of the bacteria in the sample. The present invention also features a biosensor for detecting the presence or absence of bacteria in a sample. | 2014-10-30 |
| 20140322791 | PROCESS TO PREPARE A STABLE THROMBIN COMPOSITION - The stable thrombin composition comprises purified thrombin, human albumin and a neutral salt, the resulting product being stable when stored as a lyophilisate or frozen and is adjusted to a nominal strength of 500 IU of thrombin or more per ml of solution, the human albumin being in a concentration of over 0.05% (w/v) and preferably between 0.1% (w/v) and 1% (w/v). | 2014-10-30 |
| 20140322792 | CYTOCHROME P450 OXYGENASES - Nucleic acids encoding cytochrome P450 variants are provided. The cytochrome P450 variants of have a higher alkane-oxidation capability, alkene-oxidation capability, and/or a higher organic-solvent resistance than the corresponding wild-type or parent cytochrome P450 enzyme. A preferred wild-type cytochrome P450 is cytochrome P450 BM-3. Preferred cytochrome P450 variants include those having an improved capability to hydroxylate alkanes and epoxidate alkenes comprising less than 8 carbons, and have amino acid substitutions corresponding to V78A, H236Q, and E252G of cytochrome P450 BM-3. Preferred cytochrome P450 variants also include those having an improved hydroxylation activity in solutions comprising co-solvents such as DMSO and THF, and have amino acid substitutions corresponding to T235A, R471A, E494K, and S1024E of cytochrome P450 BM-3. | 2014-10-30 |
| 20140322793 | NOVEL DNA POLYMERASES - Provided are various novel DNA polymerases. Provided is a DNA polymerase comprising an amino acid sequence modified from the amino acid sequence of SEQ ID NO: 8 by inserting nine amino acids “-A | 2014-10-30 |
| 20140322794 | SUBSTRATES FOR COVALENT TETHERING OF PROTEINS TO FUNCTIONAL GROUPS OR SOLID SURFACES - The present invention provides haloalkane substrates, and linkers for connecting such substrates to functional elements (e.g., tags, labels, surfaces, etc.). Substrates and linkers described herein find use, for example, in labeling, detection, and immobilization of proteins, cells, and molecules. In particular, the linkers provided herein find use within substrates for dehalogenase variants that form covalent bonds with their haloalkane substrates. | 2014-10-30 |
| 20140322795 | Alpha-Amylase Variants - The invention relates to a variant of a parent Termamyl-like alpha-amylase, which variant exhibits altered properties, in particular reduced capability of cleaving a substrate close to the branching point, and improved substrate specificity and/or improved specific activity relative to the parent alpha-amylase. | 2014-10-30 |
| 20140322796 | CELLOBIOHYDROLASE VARIANTS - The invention relates to recombinant expression of variant forms of C1 CBH1a and homologs thereof, having improved thermostability, low-pH tolerance, specific activity and other desirable properties. Also provided are methods for producing ethanol and other valuable organic compounds by combining cellobiohydrolase variants with cellulosic materials. | 2014-10-30 |
| 20140322797 | Method for Removing Endotoxin from Proteins - Disclosed is a method for removing endotoxin from proteins. Also disclosed are products made by using the method. The method may be used, for example, to produce endotoxin-free lactoferrin. Bovine milk-derived lactoferrin may be produced in commercial quantities by the method, and endotoxin-free bovine lactoferrin may be used for a variety of therapeutic uses, including improving wound healing. | 2014-10-30 |
| 20140322798 | INHIBITION OF METHANE PRODUCTION DURING ANAEROBIC REDUCTIVE DECHLORINATION - This method of restricting methane production in methanogenic bacteria, by the use of the enzyme and coenzyme inhibitors, works during anaerobic reductive dechlorination. Various compounds such as, but not limited to, red yeast rice, vitamin B10 derivatives, and ethanesulfonates are utilized to disrupt these different enzyme and coenzyme systems responsible for the production of methane. This method affects the competition of the methanogen and halo bacteria for the organic hydrogen donors that are injected in the soil and groundwater system during the remediation process. | 2014-10-30 |
| 20140322799 | METABOLICALLY ENHANCED CYANOBACTERIUM WITH SEQUENTIALLY INDUCIBLE PRODUCTION GENES FOR THE PRODUCTION OF A FIRST CHEMICAL COMPOUND - This invention provides a metabolically enhanced cyanobacterium for the production of a chemical compound of interest, having at least two first production genes encoding first biocatalysts for the production of the first chemical compound. One of the two first production genes is under the transcriptional control of a first promoter for the first production gene, whereas the other of the two first production genes is under the transcriptional control of a second promoter for the first production gene. The first promoter and second promoter are separately inducible under different conditions and the at least two first biocatalysts catalyze the same chemical reaction. Metabolically enhanced cyanobacteria according to the present invention allow prolonged production of first chemical compounds. Further provisions of the present invention include, inter alia, a method for producing the metabolically enhanced cyanobacterium and a method for producing a first chemical compound by culturing the metabolically enhanced cyanobacterium. | 2014-10-30 |
| 20140322800 | OXIDIZED CARDIOLIPIN AND USES TO DETECT CARDIOLIPIN ANTIBODIES - Compositions, methods and devices for the detection of anti-lipoidal antibodies and the diagnosis of disease, for example, syphilis, are described. In particular, oxidized cardiolipins, which may be conjugated with a variety of attachment molecules, such as BSA, KLH, biotin, synthetic protein MAPS, IgY, streptavidin, or avidin, are described. Such oxidized cardiolipin, alone or complexed with one or more attachment molecules, are useful to detect anti-lipoidal antibodies (such as IgG and IgM antibodies) in subjects, for example, when used in ELISA plates. ELISA plates are described that permit the detection of anti-lipoidal antibodies and that permit the co-detection of nontreponemal and treponemal antibodies in biological samples. | 2014-10-30 |
| 20140322801 | OPTICAL IMAGING BASED ON VISCOELASTIC FOCUSING - An apparatus arranged for examining particles, comprising: a cartridge having at least one microchannel, a viscoelastic fluid flowing in the microchannel, the fluid comprising a suspension of particles, thereby effecting alignment of the particles in at least one-dimensional array parallel to the fluid flow, and an optical magnifying means generating an image of the particles in the microchannel. | 2014-10-30 |
| 20140322802 | APPARATUS FOR MEASURING BLOOD CELLS AND IMMUNITY FROM WHOLE BLOOD - In conventional apparatuses for measuring blood cells and immunity from whole blood, the CRP cell is used for the final cleaning of a nozzle, and CRP measurement—cleaning of the CRP cell—final cleaning of nozzle are performed in this order in the CRP cell. In contrast, in the present invention, an exclusive cleaning chamber A for immunity measurement is further provided for the cleaning nozzle after completion of dispensing the specimen and the reagent(s) for CRP measurement to the CRP cell. Thus, a configuration is adapted wherein the outer surface and inner surface of the nozzle are cleaned in the cleaning chamber A for immunity measurement while the immunity measurement is performed in CRP cell. Since the final cleaning of the nozzle is performed in parallel with the CRP measurement, the time conventionally necessary for the final cleaning of the nozzle can be shortened. | 2014-10-30 |
| 20140322803 | Carbon Sequestration Methods and Systems, and Compositions Produced Thereby - Aspects of the invention include methods of removing carbon dioxide (CO | 2014-10-30 |
| 20140322804 | PHOTOBIOREACTORS AND CULTURE BAGS FOR USE THEREWITH - There is provided a culture bag for use in a photobioreactor. The bag can comprise at least one wall having at least one inlet disposed at a first end portion of the at least one wall or adjacently thereto. The at least one wall defines an internal chamber for receiving a culture medium. The bag also comprises at least one injector for injecting a gas inside the bag, the at least one injector being disposed at a second end portion of the at least one wall or adjacently thereto. The bag also comprises at least one outlet for harvesting a content of the bag, the at least one outlet being disposed at the second end portion of the at least one wall or adjacently thereto. The bag can be translucent or transparent and be effective for holding and sealingly maintaining the culture medium inside the bag and inside the photobioreactor. There is also provided a photobioreactor and a photobioreator modular system. | 2014-10-30 |
| 20140322805 | System and Method for Using a Pulse Flow Circulation for Algae Cultivation - A device for generating a pulsed flow in a channel containing a circulating algal culture can include a plate that is pivotably mounted on the channel and an activator. A pulsed flow is generated in the channel by first positioning the plate to impede the flow of circulating algal culture and then rotating the plate to a submerged position. The pulsed flow can be employed to counteract the negative effects of bio-fouling on algae cultivation equipment. In another arrangement, a device for generating a pulsed flow in a sloped raceway that is in fluid communication with a sump can include a gate. In different embodiments, the gate can operate as a so-called “pinch gate” or as a so-called “overflow gate.” In another aspect, a variable rate pump, such as a centrifugal pump, a screw pump or an airlift pump, is described for establishing a pulsed flow in a channel. | 2014-10-30 |
| 20140322806 | SPHEROID CELL CULTURE WELL ARTICLE AND METHODS THEREOF - A spheroid cell culture article including:
| 2014-10-30 |
| 20140322807 | METHOD FOR EXTRACTING NUTRIENTS FROM ORGANIC MATERIALS - Processes for extracting and recovering nutrients from organic wastes to create a cell culture broth for microorganisms involve the main steps of mixing, solid/liquid separation, optimization, and sterilization. In an embodiment, the method for converting organic waste material into a cell culture broth or growth media includes: (a) mixing an organic waste material with one or more solvent to create a mixture of liquids and solids under substantially turbulent conditions; (b) separating the mixture of liquids and solids into a liquid stream and solid stream; and (c) sterilizing the liquid stream, whereby the cell culture broth or growth media comprises the sterilized liquid stream. | 2014-10-30 |
| 20140322808 | POPULATIONS OF HEMATOPOIETIC PROGENITORS AND METHODS OF ENRICHING STEM CELLS THEREFOR - There is described herein a method of enriching a population of stem cells for hematopoietic progenitors. The method comprises inducing hematopoietic differentiation in a population of human embryonic stem cells or human induced pluripotent stem cells; sorting the population based on expression of CD43 and at least one of CD34, CD31 and CD144; and selecting a fraction that is at least one of CD34 | 2014-10-30 |
| 20140322809 | Use of a Rock Inhibitor to Sustain Primary Human Keratinocytes in a Proliferative State - Disclosed herein is the finding that treatment with a ROCK inhibitor increases proliferation and induces immortalization of primary keratinocytes. Accordingly, provided is a method of immortalizing primary keratinocytes by exposure to a ROCK inhibitor. Also provided are immortalized primary keratinocytes produced by the described method, as well as organotypic tissue equivalents and cell cultures comprising the immortalized primary keratinocytes. Furthermore, ROCK inhibitor-treated cells show a greatly increased ability to support viral DNA replication of both “low risk” and “high risk” HPV genomes, indicating that ROCK inhibitors will be useful for studying the life cycles of a wide range of HPVs. | 2014-10-30 |
| 20140322810 | NATURAL AND SYNTHETIC COMPOUNDS FOR TREATING CANCER AND OTHER DISEASES - This invention provides a method of synthesizing new active compounds for pharmaceutical uses including cancer treatment, wherein the cancers comprise breast, leukocytic, liver, ovarian, bladder, prostatic, skin, bone, brain, leukemia, lung, colon, CNS, melanoma, renal, cervical, esophageal, testicular, spleenic, kidney, lymphatic, pancreatic, stomach and thyroid cancers. This invention is an anti-adhesion therapy which uses the compound as a mediator or inhibitor of adhesion proteins and angiopoietins. It inhibits excess adhesion and inhibits cell attachment. It modulates angiogenesis. The compounds also use as mediator of cell adhesion receptor, cell circulating, cell moving and inflammatory diseases. The compounds are attached with angeloyl, acetyl, tigloyl, senecioyl, Crotonoyl, 3,3-Dimethylartyloyl, Cinnamoyl, Pentenoyl, Hexanoyl, benzoyl, Ethylbutyryl, benzoyl, dibenzoyl, alkanoyl, alkenoyl, benzoyl alkyl substituted alkanoyl, ethanoyl, propanoyl, propenoyl, butanoyl, butenoyl, pentanoyl, hexenoyl, heptanoyl, heptenoyl, octanoyl, octenoyl, nonanoyl, nonenoyl, decanoyl, decenoyl, propionyl, 2-propenoyl, 2-butenoyl, Isobutyryl, 2-methylpropanoyl, 2-ethylbutyryl, ethylbutanoyl, 2-ethylbutanoyl, butyryl, (E)-2,3-Dimethylacryloyl, (E)-2-Methylcrotonoyl, 3-cis-Methyl-methacryloyl, 3-Methyl-2-butenoyl, 3-Methylcrotonoyl, 4-Pentenoyl, (2E)-2-pentenoyl, Caproyl, 5-Hexenoyl, Capryloyl, Lauroyl, Dodecanoyl, Myristoyl, Tetradecanoyl, Oleoyl, O—C(2-18) Acyl. | 2014-10-30 |
| 20140322811 | MEDIUM COMPOSITION FOR REJUVENATING STEM CELLS - The present invention relates to a medium composition for transforming stem cells from an aged person into young stem cells, and more particularly to a medium composition for culturing stem cells, which is used to rejuvenate stem cells from an aged person so as to have characteristics similar to those of the stem cells of young people, and to a method for rejuvenating stem cells, which comprises culturing stem cells from an aged person in the medium composition. According to the present invention, even mesenchymal stem cells collected from over 60 years old patients can be transformed into young mesenchymal stem cells having high differentiation ability, high telomerase activity, and high ability to express stem cell markers. Thus, the present invention can significantly increase the efficacy of cell therapy employing mesenchymal stem cells. | 2014-10-30 |
| 20140322812 | Mutant Receptors and Their Use in a Nuclear Receptor-Based Inducible Gene Expression System - This invention relates to the field of biotechnology or genetic engineering. Specifically, this invention relates to the field of gene expression. More specifically, this invention relates to novel substitution mutant receptors and their use in a nuclear receptor-based inducible gene expression system and methods of modulating the expression of a gene in a host cell for applications such as gene therapy, large scale production of proteins and antibodies, cell-based high throughput screening assays, functional genomics and regulation of traits in transgenic organisms. | 2014-10-30 |
| 20140322813 | METHODS AND COMPOSITIONS FOR ENHANCING THE EFFICACY AND SPECIFICITY OF RNA SILENCING - The present invention provides methods of enhancing the efficacy and specificity of RNA silencing. The invention also provides compositions for mediating RNA silencing. In particular, the invention provides siRNAs, siRNA-like molecules, shRNAs, vectors and transgenes having improved specificity and efficacy in mediating silencing of a target gene. Therapeutic methods are also featured. | 2014-10-30 |
| 20140322814 | MEANS AND METHOD FOR DETERMINING CHEMICAL OXYGEN DEMAND - The invention relates to a means and a method for the photometric determination of the chemical oxygen demand of chloride-containing samples. | 2014-10-30 |
| 20140322815 | PAIRING AND SYNCHRONIZING A MOBILE PHONE APPLICATION WITH A HANDHELD GLUCOSE METER - A diabetes management system includes a handheld medical device, a mobile computing device, and a diabetes management application. The handheld medical device is configured to determine, in response to a port receiving a test strip, whether an auto-send feature is enabled on the handheld medical device, determine whether the handheld medical device is paired with a mobile computing device, and selectively instruct a wireless transceiver to establish a wireless connection and communicate a glucose measurement and identifying information to the mobile computing device. The mobile computing device is configured to execute the diabetes management application. The diabetes management application is configured to process a plurality of glucose measurements and identifying information associated with each of a plurality of glucose measurements. | 2014-10-30 |
| 20140322816 | PORTABLE EXPLOSIVE OR DRUG DETECTION SYSTEM - A portable chemical analytical apparatus to analyze a test swipe includes a heater to warm the test swipe to a predetermined temperature; a clamp to secure the test swipe to the heater; one or more pumps to dispense one or more chemicals onto the test swipe from a disposable cartridge; a fan to remove chemical vapors rising a predetermined distance from the test swipe; and a camera to capture an image of the test swipe for automated analysis. | 2014-10-30 |
| 20140322817 | Mass Spectrometer - A mass spectrometer is disclosed comprising an Electron Transfer Dissociation cell. Positive analyte ions are fragmented into fragment ions upon colliding with singly charged negative reagent ions with the cell. The cell comprises a plurality of ring electrodes which form a spherical trapping volume. Ions experience negligible RF heating over the majority of the trapping volume which enables the kinetic energy of the analyte and reagent ions to be reduced to just above thermal temperatures. An Electron Transfer Dissociation cell having an enhanced sensitivity is thereby provided. Fragment ions created within the cell may be cooled and may be transmitted onwardly to an orthogonal acceleration Time of Flight mass analyser enabling a significant improvement in the resolution of the mass analyser to be obtained. | 2014-10-30 |
| 20140322818 | Nanocrystal Based Biomolecule Detection - A new signal amplification method exploiting the dense atom packing in metallic nanocrystals has been developed for detecting target substances. By dissolving nanocrystals to individual ions that are stoichiometrically converted to chromophores and quantified photometrically, extremely high signal amplification can be achieved. Signal amplification is fully determined by the total number of atoms in the nanocrystals bound to a single target molecule. The disclosed nanocrystal amplification method can be implemented with a rich selection of metal/metal oxide nanocrystals and metal-reactive chromogenic substrates. The chromogenic reactions can be either solution-based or surface-based and performed in aqueous or organic phase, supporting a variety of assay formats | 2014-10-30 |
| 20140322819 | DETECTION AND/OR QUANTITATION OF ENDOTOXIN - Detection and/or quantitation of endotoxin using biolayer interferometry is disclosed. | 2014-10-30 |
| 20140322820 | MATERIALS AND METHODS FOR ISOLATING PHOSPHOPEPTIDES - Protein phosphorylation is a major post-translational modification and it plays a pivotal role in numerous cellular functions. We present a composition that includes a soluble nanopolymer core functionalized with groups having an affinity for either metal ion or metal oxides which can be used for phosphopeptide enrichment. Exemplary compounds including PolyMAC-Zr, PolyMAC-Fe and PolyMAC-Ti demonstrate outstanding reproducibility, exceptional sensitivity, fast chelation time, and high phosphopeptide recovery from standard mixtures that include phosphorylated peptides. The composition can be used for phosphoproteome isolation from samples of medicinal, diagnostic or biological interest such as malignant breast cancer cells. Such compositions were used for the quantitative analysis of the changes in the tyrosine phosphoproteome in highly invasive breast cancer cells after induction of Syk kinase, a potent suppressor of tumor growth and metastasis. The composition and method disclosed herein offers an efficient and widely applicable tool for phosphoproteomics. | 2014-10-30 |
| 20140322821 | MOLECULE CAPABLE OF BINDING TO ADRENOCORTICOTROPIC HORMONE, AND USE THEREOF - The present invention relates to a molecule capable of binding to adrenocorticotropic hormone (ACTH) with high affinity. The present invention also relates to use of the molecule for detection and/or purification of ACTH. | 2014-10-30 |
| 20140322822 | Determination of a Midregional Proadrenomedullin Partial Peptide in Biological Fluids for Diagnostic Purposes, and Immunoassays for Carrying out Such a Determination - Method for the determination of adrenomedullin immunoreactivity in biological fluids for diagnostic purposes, in particular in sepsis, cardiac and cancer diagnosis, in which the midregional partial peptide (mid-proAM; SEQ ID NO:3) of proadrenomedullin, which comprises the amino acids (45-92) of the complete preproadrenomedullin (pre-proAM; SEQ ID NO:1), is measured in particular by means of an immunoassay which operates with at least one labelled antibody which specifically recognizes a sequence of mid-proAM. | 2014-10-30 |
