42nd week of 2011 patent applcation highlights part 41 |
Patent application number | Title | Published |
20110256539 | Methods for Detecting Tumor Origin Based on MUC1, MUC2, and CK-17 Expression Levels - Methods and compositions for detecting tumor origin are disclosed. In certain embodiments, the origin is determined by detecting expression levels of pan-epithelial membrane mucin (MUC1), intestinal-type secretory mucin (MUC2), cytokeratin 17 (CK17), or a combination thereof. Also disclosed are methods for aiding in the determination of an appropriate course of treatment for a subject with a tumor, or for predicting a therapeutic outcome of a subject, based on the expression levels of MUC1, MUC2, CK17, or a combination thereof. Kits for use in each of these methods are also provided. | 2011-10-20 |
20110256540 | UNRESTRICTED MUTAGENESIS AND CLONING METHODS - The invention relates to methods for amplifying, modifying, mutating and cloning DNA of any size. These methods comprise a series of PCR reactions, which are punctuated by ligation reactions. | 2011-10-20 |
20110256541 | COMPOSITIONS FOR USE IN IDENTIFICATION OF BACTERIA - The present invention provides compositions, kits and methods for rapid identification and quantification of bacteria by molecular mass and base composition analysis. | 2011-10-20 |
20110256542 | Droplet Actuator Devices, Systems, and Methods - The invention relates to certain novel approaches to reducing or eliminating the movement of contaminants from one droplet to another on a droplet actuator via liquid filler fluid. In one application, droplet actuators are used to conduct genetic analysis using polymerase chain reaction (PCR) techniques. The invention addresses the need for improved methods of performing PCR on a droplet actuator that provide for optimum amplification and detection of a sample target. | 2011-10-20 |
20110256543 | USE OF NOVEL CYTOKINE RECEPTORS AS BIOMARKERS AND THERAPEUTIC TARGETS IN HUMAN CANCER - Nucleic acids encoding erythropoietin isoforms are described herein, as well as the encoded isoforms, methods of detecting the same, and methods of screening for and treating cancer. | 2011-10-20 |
20110256544 | MOLECULES INTERACTING WITH CASL (MICAL) POLYNUCLEOTIDES, POLYPEPTIDES, AND METHODS OF USING THE SAME - The present invention provides MICAL and MICAL-Like polypeptides and polynucleotides. Also provided are methods that for identifying agents that affect axon growth and placement. Furthermore, provided herein are methods for affecting axon growth and placement. | 2011-10-20 |
20110256545 | mRNA expression-based prognostic gene signature for non-small cell lung cancer - A non-small cell lung cancer postoperative survival prognosticator comprising a detection mechanism consisting of 15-gene, 12-gene, and 16-gene signature and methods of use. Also provided are the identification of various subsets from the 25 prognostic signature genes with potential of operative survival prognosticator for non-small cell lung cancer patients in all tumor stage and early stage and potential for chemoresponse with a method of use. | 2011-10-20 |
20110256546 | METHODS AND COMPOSITIONS FOR THE DIAGNOSIS AND TREATMENT OF CANCER RESISTANT TO ANAPLASTIC LYMPHOMA KINASE (ALK) KINASE INHIBITORS - Compositions and methods for the diagnosis and treatment of a cancer that is resistant to at least one anaplastic lymphoma kinase (ALK) kinase inhibitor are provided herein. The present invention is based on the discovery of mutations within ALK that confer resistance to at least one ALK kinase inhibitor. Polynucleotides and polypeptides having at least one ALK inhibitor resistance mutation are provided and find use in methods and compositions useful in the diagnosis, prognosis, and/or treatment of diseases associated with aberrant ALK activity, more particularly, those that are resistant to at least one ALK kinase inhibitors. Methods and compositions are also provided for the identification of agents that can inhibit the kinase activity and/or reduce the expression level of the ALK resistance mutants. | 2011-10-20 |
20110256547 | MicroRNAa (miRNA) AS BIOMARKERS FOR DIAGNOSING DIFFERENT GRADES OF GLIOMAS AND PATHWAYS OF GLIOMA PROGRESSION - This invention relates to a method for detecting, (i) malignant astrocytoma from normal brain tissue (ii) glioblastoma and anaplastic astrocytoma (iii) primary glioblastoma and secondary glioblastoma (iv) progressive pathway and denovo pathway comprises determining the level of expression of miRNAs listed in table 2, 3, 4, 5, wherein a higher or lower level of expression of miRNAs in the test sample as compared to the control sample differentiates and kit for characterizing a) malignant astrocytoma from normal brain tissue cell comprising reagent capable of specifically detecting the level of expression of the genes of miRNAs and instructions for using said kit for characterizing malignant astrocyoma from normal brain tissue cells b) glioblastoma from anaplastic astrocytoma comprising reagent capable of specifically detecting the level of expression of the genes of miRNAs and instructions for using said kit for characterizing glioblastoma from anaplastic astrocyoma c) primary glioblastoma from secondary glioblastoma comprising reagent capable of specifically detecting the level of expression of the genes of miRNAs and instructions for using said kit for characterizing primary glioblastoma from secondary glioldastoma, d) progressive pathway from denovo pathway comprising reagent capable of specifically detecting the level of expression of the genes of miRNAs and instructions for using said kit for characterizing progressive pathway from denovo pathway. | 2011-10-20 |
20110256548 | METHOD FOR OBTAINING PANCREATIC PROGENITOR CELL USING NEPH3 - The present invention provides markers that can selectively distinguish pancreatic progenitor cells. The present invention also provides methods for distinguishing pancreatic progenitor cells by using the markers as an indicator, and reagents to be used in the methods. | 2011-10-20 |
20110256549 | Novel reagents for directed biomarker signal amplification - Described herein are methods, compositions and articles of manufacture involving neutral conjugated polymers including methods for synthesis of neutral conjugated water-soluble polymers with linkers along the polymer main chain structure and terminal end capping units. Such polymers may serve in the fabrication of novel optoelectronic devices and in the development of highly efficient biosensors. The invention further relates to the application of these polymers in assay methods. | 2011-10-20 |
20110256550 | Novel reagents for directed biomarker signal amplification - Described herein are methods, compositions and articles of manufacture involving neutral conjugated polymers including methods for synthesis of neutral conjugated water-soluble polymers with linkers along the polymer main chain structure and terminal end capping units. Such polymers may serve in the fabrication of novel optoelectronic devices and in the development of highly efficient biosensors. The invention further relates to the application of these polymers in assay methods. | 2011-10-20 |
20110256551 | SYSTEMS AND DEVICES FOR ANALYSIS FOR SAMPLES - Systems and methods for analysis of samples, and in certain embodiments, microfluidic sample analyzers configured to receive a cassette containing a sample therein to perform an analysis of the sample are described. The microfluidic sample analyzers may be used to control fluid flow, mixing, and sample analysis in a variety of microfluidic systems such as microfluidic point-of-care diagnostic platforms. Advantageously, the microfluidic sample analyzers may be, in some embodiments, inexpensive, reduced in size compared to conventional bench top systems, and simple to use. Cassettes that can operate with the sample analyzers are also described. | 2011-10-20 |
20110256552 | METHOD FOR PREPARING A SUBSTRATE FOR IMMOBILIZING A CELL, SAID SUBSTRATE AND USES THEREOF - The present invention relates to a method of preparation of a solid substrate capable of immobilizing at least one cell and/or at least one part of a cell, said method comprising a step consisting of fixing, to said solid substrate, a fusogenic compound capable of being inserted in cell membranes. The present invention also relates to a method for immobilizing at least one cell and/or at least one part of a cell using the solid substrate thus prepared, said solid substrate and its uses in the area of biomedical diagnostics or health monitoring of biological fluids or intended for human or animal use. | 2011-10-20 |
20110256553 | Methods - A method for assessing the effect of a test compound on LRRK2 in a cell-based system, the method comprising the steps of a) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the phosphorylation state of Ser910 and/or Ser935 of the LRRK2; and/or b) assessing the effect of exposing the cell-based system comprising LRRK2 to the test compound on the binding of the LRRK2 to a 14-3-3 polypeptide. The method is considered to be useful in assessing the effect of putative LRRK2 inhibitors in cell based systems, including in vivo systems. | 2011-10-20 |
20110256554 | METHODS FOR PREDICTING PREGNANCY OUTCOME IN A SUBJECT BY HCG ASSAY - The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample. | 2011-10-20 |
20110256555 | USE OF CXCR4 PROTEIN EXPRESSION ON THE SURFACE OF STEM CELLS AS A MARKER FOR TUMOR TROPIC POTENTIAL - The present invention relates to tumor tropic stem cells, and particularly to neural stem cells, and their use as delivery vehicles for therapeutic gene products to neoplastic foci. The stem cells with tumor tropic potential are selected based on the stem cells exhibiting CXCR4 receptors or an affinity for the chemokine SDF-1. The stem cells may additionally exhibit markers characteristic of astrocytic progenitors. The stem cells may be administered as part of a treatment regimen including the chemokine SDF-1. | 2011-10-20 |
20110256556 | Humanized FcgR Mice - Genetically modified non-human animals and methods and compositions for making and using them are provided, wherein the genetic modification comprises a deletion of the endogenous low affinity FcγR locus, and wherein the mouse is capable of expressing a functional FcRγ-chain. Genetically modified mice are described, including mice that express low affinity human FcγR genes from the endogenous FcγR locus, and wherein the mice comprise a functional FcRγ-chain. Genetically modified mice that express up to five low affinity human FcγR genes on accessory cells of the host immune system are provided. | 2011-10-20 |
20110256557 | IDENTIFYING PARATHYROID HORMONE AGONISTS AND ANTAGONISTS - Provided herein are methods of screening for an agent that is a PTH agonist or antagonist. For example, provided is a method of screening for an agent that is a PTH agonist or antagonist, the method comprising contacting a cell with LRP6 and the agent to be screened, wherein the cell comprises a PTH1R, and determining the level of LRP6 binding to the PTH1R. An increased level of LRP6 binding to the PTH1R compared to a control indicates the agent is a PTH agonist. A decreased level of LRP6 binding to the PTH1R compared to a control indicates the agent is a PTH antagonist. Also provided are methods of treating a skeletal disorder in a subject, wherein the skeletal disorder is characterized by proliferative bone growth or reduced bone density. | 2011-10-20 |
20110256558 | MEASURING LEVELS OF FIBROCYSTIN-L - Nucleic acids encoding fibrocystin-L polypeptides and fibrocystin-L polypeptides are provided. Antibodies against the polypeptides, vectors and host cells containing the nucleic acids, methods for using the nucleic acids and polypeptides, and compositions and articles of manufacture also are provided. | 2011-10-20 |
20110256559 | Method for Detecting Soluble Amyloid Precursor Protein (APP) Alpha and/or Soluble APP Beta - The present invention relates to a method for detection or quantification of sAPPα and/or sAPPβ in a sample and their use for diagnosing and/or monitoring a neurodegenerative disease in a subject in need thereof and kit for use in said method. | 2011-10-20 |
20110256560 | METHODS AND COMPOSITIONS FOR THE DETECTION OF OVARIAN CANCER - Provided is a method of screening for, diagnosing or detecting ovarian cancer in a subject comprising (a) determining a level of mdogen-2 in a test sample from the subject, and (b) comparing the level of mdogen-2 in the test sample with a control, where detecting an increase in the level of mdogen-2 in the test sample compared to the control is indicative of ovarian cancer in the subject. | 2011-10-20 |
20110256561 | LIVER DISEASE MARKER, METHOD AND APPARATUS FOR MEASURING THE SAME, AND METHOD FOR ASSAYING PHARMACEUTICAL PREPARATION - A normal person (i.e. a control) and liver diseases such as drug induced liver injury, an asymptomatic hepatitis B carrier, an asymptomatic hepatitis C carrier, chronic hepatitis B, chronic hepatitis C, liver cancer, a nonalcoholic fatty liver disease (NAFLD), nonalcoholic steatohepatitis (NASH), and simple steatosis (SS) are identified by measuring the concentrations of γ-Glu-X (X represents an amino acid or an amine) peptides or the levels of AST or ALT in blood and carrying out, for example, a multiple logistic regression based on the measured value. | 2011-10-20 |
20110256562 | METHODS FOR DIAGNOSING HEART FAILURE AND OTHER CARDIAC DISEASES - The invention relates to methods of diagnosing the severity of heart failure or a cardiac dysfunction in a subject. The invention further relates to monitoring the severity of heart failure in a subject and determining the prognosis of a subject that has suffered from heart failure. This invention also relates methods to identify patients at risk for cardiac dysfunction when exposed to cardiotoxic chemotherapy agents. | 2011-10-20 |
20110256563 | METHOD FOR DETECTING LYSOSOMAL STORAGE DISEASES - A method for detecting lysosomal storage diseases including the steps of performing an assay for a single species of glycosaminoglycan contained in a specimen and correlating results of the assay with lysosomal storage diseases. A body fluid such as urine or blood can be employed as a specimen. The assay can be performed by use of a polypeptide that is capable of specifically binding to a glycosaminoglycan-containing molecule. The polypeptide may be an antibody, or a polypeptide having an antigen-binding site of an antibody. | 2011-10-20 |
20110256564 | METHODS, REAGENTS AND KITS FOR LUCIFERASE ASSAY - The invention relates to methods, reagents and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel luciferase assay system with reduced background luminescence to allow for increased detection sensitivity. Provided is a method of detecting luciferase activity in a sample using coelenterazine or an analog thereof as a substrate, comprising: (a) initiating luciferase-catalyzed luminescence production by contacting said sample with a luciferase detection reagent to yield a reaction mixture, said reagent comprising coelenterazine and at least one iodide source in an amount sufficient to reduce the autoluminescence of said coelenterazine, (b) incubating said reagent mixture under conditions suitable to produce luminescence, and (c) measuring the luminescence produced. Also provided are detections reagents and kits for use in such a method. | 2011-10-20 |
20110256565 | METHODS FOR DIAGNOSIS AND TREATMENT OF NEURODEGENERATIVE DISEASES OR DISORDERS - The present invention provides methods that are useful for the diagnosis of neurodegenerative disease or disorder and for the screening of compounds or therapeutic agents for treating a neurodegenerative disease or disorder. The methods pertain in part to the correlation of a neurodegenerative disease or disorder with abnormal or altered endoplasmic reticulum-mitochondrial-associated membranes (ER-MAM) integrity. | 2011-10-20 |
20110256566 | Determination of biological material ingredients - The invention relates to a method for analysing ingredients, in particular lipids and/or vitamins and biological materials with a concentration of the biological material ingredients, to uses of relevant organic solvents or organic solvent mixtures, and to a spectrophotometer for measuring biological material ingredients. It is proposed to treat the biological materials with at least one organic solvent which extracts the ingredients, to convert the biological materials to a solidified form during the extraction, with the result that a solidified sediment and a liquid organic phase as the supernatant are formed, and to examine the extracted ingredients in the supernatant. | 2011-10-20 |
20110256567 | FLUORESCENT CYCLIC PEPTIDES, PREPARATION METHOD THEREOF AND USE OF THESE PEPTIDES FOR MEASURING THE ENZYMATIC ACTIVITY OF A PROTEASE ENZYME - The invention pertains to a cyclic peptide comprising the following sequence: | 2011-10-20 |
20110256568 | METHODS FOR THE DETECTION AND/OR QUANTIFICATION OF GRAM POSITIVE BACTERIAL CONTAMINANTS - The invention provides methods and compositions for the detection and/or quantification of a Gram positive bacterial contaminant in a sample. In particular, the invention provides hemocyte-based preparations, methods of making such hemocyte-based preparations, and methods of using such hemocyte-based preparations for the detection and/or quantification of the Gram positive bacterial contaminant. | 2011-10-20 |
20110256569 | COMPLEX OF A CHAPERONE WITH BETA-AMYLOID AND METHODS EMPLOYING THIS COMPLEX - A chaperone protein Q2 and β-amyloid can form a complex. This complex can be detected in a biological sample, such as, for example, tissues or fluids from a mammal. Q2 levels can also be detected in a biological sample. A method for determining the Q2 level in a biological sample and comparing that level to a normal Q2 level can be used to detect, screen, diagnose, or otherwise determine a person's susceptibility to Alzheimer's disease such as, for example, the presence or absence of Alzheimer's disease, of symptoms of this disease, of factors leading to or associated with this disease, of likelihood of developing this disease, and the like. In one embodiment, a decline in Q2 level correlates to an increased likelihood of developing Alzheimer's disease. In another embodiment, a decline in Q2 level correlates to an increase in βamyloid aggregation. The method may further include screening for an apolipoprotein E genotype, which is associated with Alzheimer's disease. | 2011-10-20 |
20110256570 | METHOD AND KIT FOR POSITIVELY DETECTING TOXIC MATERIAL - A method of positively detecting toxic materials within a sample, the method including contacting sub-mitochondrial particles having competent mitochondrial enzymes formed from inner membranes of mitochondria, an electron donor which transmits electrons to an electron transfer system of the sub-mitochondrial particles and the sample, and forming reaction resultants, adjusting a pH of a pH indicator which change color according to a change in pH, adding the pH indicators to each reaction resultant and identifying color changes of the pH indicator. Also provided is a kit for positively detecting toxic materials within a sample. | 2011-10-20 |
20110256571 | PROTEINS - An amino acid sequence is described. The amino acid may comprise a signal sequence that is SEQ ID NO. 1a (or a variant or homologue or derivative or fragment thereof) that is expressed as a fusion protein that comprises a protein of interest. The signal sequence directs secretion of the protein of interest. The protein of interest may be a heterologous protein. Secretion of the fusion protein aids purification. | 2011-10-20 |
20110256572 | BIOLOGICAL SAMPLE DISCRIMINATION APPARATUS, BIOLOGICAL SAMPLE DISCRIMINATION METHOD, AND BIOLOGICAL SAMPLE DISCRIMINATION PLATE - A plate on which a channel pattern is formed, which channel pattern includes a first channel into which a buffer agent is injected, and a second channel having, in a portion thereof, a quantification part that has a portion common to the first channel and holds a predetermined amount of a biological sample, the biological sample being injected into the channel including the quantification part, and the plate is rotated at a high speed by a filling unit to fill the buffer agent in the first channel, and thereafter, the second channel is pressurized by a discrimination unit to fill the biological sample in the second channel, and simultaneously, a predetermined amount of the biological sample is added into the buffer agent. Therefore, when performing discrimination of the biological sample, a discrimination result can be obtained accurately in a short time without the necessity of complicated preparation works. | 2011-10-20 |
20110256573 | METHOD AND APPARATUS FOR DETERMINING AT LEAST ONE HEMOGLOBIN RELATED PARAMETER OF A WHOLE BLOOD SAMPLE - A method and apparatus for determining at least one hemoglobin related parameter of a whole blood sample is provided. The method includes the steps of: a) depositing the sample into an analysis chamber adapted to quiescently hold the sample for analysis, the chamber defined by an interior surface of a first panel, and an interior surface of a second panel, and the chamber has a height extending between the interior surfaces of the panels, wherein the chamber is configured to increase the oxygenation state of the sample to a substantially oxygenated state within a predetermined amount of time after entry into the chamber; b) imaging the at least one red blood cell contacting the interior surfaces, and producing image signals; c) determining an optical density of at least a portion of the imaged red blood cell contacting both interior surfaces; and d) determining the at least one hemoglobin related parameter of the red blood cell contacting the interior surfaces, using the determined optical density and a molar extinction coefficient for oxygenated hemoglobin. | 2011-10-20 |
20110256574 | Microfluidic Continuous Flow Device - A microfluidic continuous flow device comprising a channel which comprises a first and a second area wherein the first area of the channel is a compartment which is defined by partitioning elements and the second area of the channel is a space outside the compartment; wherein through passages which are formed between the partitioning elements are dimensioned such as to retain a biological material and optionally a sustained release composition which can be comprised in the compartment within the compartment; wherein the channel has a first inlet to the compartment through which biological material can be introduced into the compartment; a second inlet for introducing a cultivation medium into a space of the channel arranged outside of the compartment, and an outlet. The present invention further refers to methods of using the devices of the present invention and kits comprising the microfluidic continuous flow devices of the present invention. | 2011-10-20 |
20110256575 | DIGITAL SAMPLING APPARATUS AND METHODS FOR SORTING PARTICLES - A system and method for sorting a mixture of stained particles including a digital signal processor for analyzing and classifying the digital information generated from the particles and providing a sorting signal to a sorting system as a function of the analyzed and classified digital information. | 2011-10-20 |
20110256576 | RECOVERY AND DETECTION OF MICROORGANISMS FROM MIXED CELLULOSE ESTER FILTRATION SUPPORTS BY SEQUENTIAL TREATMENT WITH METHANOL AND ACETONE - The present invention is directed to the recovery of bacteria and microparasites, particularly | 2011-10-20 |
20110256577 | METHOD FOR SENSING A BIOCHEMICAL AND/OR BIOMECHANICAL PROCESS OF A BIOLOGICAL MATERIAL AND METHOD FOR ANALYZING BIOLOGICAL MATERIALS - A method for sensing a biochemical and/or biomechanical process of a biological material, comprising the steps of: disposing at least a part of a microresonator into the biological material; and before, during, or after disposing the part of the microresonator into the biological material, sensing the process of the biological material by analysis of one or more optical cavity modes of the microresonator. | 2011-10-20 |
20110256578 | Detection method using nanoaggregate-embedded beads and system thereof - The invention discloses a detection method using nanoaggregate-embedded beads and system thereof, which are characterized in that the nanoaggregate of Raman dye and metal nanoparticles is coated by an inorganic oxide to form a nanoaggregate-embedded bead, and which is then conjugated with a probe molecule to form a sensor bead. The Raman spectra of the product formed by binding of the sensor bead and an analyte in a sample is detected for determining whether the analyte exists in the sample. In embodiment, the pH of the solution of metal nanoparticles is controlled to keep at 10, and the concentration of the Raman dye is controlled to keep between 1×10 | 2011-10-20 |
20110256579 | Method for Verifying and/or Identifying Hormonally Effective Substances - The invention relates to a method for verifying and/or identifying hormonally effective substances using the pheromone system of yeast, to yeast cells for carrying out the method, and to the use thereof. The method according to the invention comprises the following steps: a) adding yeast culture medium or buffer to a sample which presumably contains a hormonally effective substance, b) thereafter contacting the sample with haploid yeast cells that possess features 1 through 3: 1. the DNA sequence coded for a heterologous hormone receptor is placed under the control of a constitutive promoter, 2. the DNA sequence coded for a yeast pheromone is placed under the control of a promoter that can be regulated by the hormone receptor, 3. a gene that is coded for a receptor for the yeast pheromone is constitutively expressed, wherein the activation of the hormone receptor by the hormonally effective substance leads to the expression and secretion of the yeast pheromone, and c) the physiological and/or morphological changes of haploid yeast cells are detected. | 2011-10-20 |
20110256580 | LC-MFR-MS-Based Method and Apparatus for Screening a New Drug Candidate - The present invention relates to a method for screening a new drug candidate using a liquid chromatography/microfluidic device/mass spectrometry system, and to a liquid chromatography/microfluidic device/mass spectrometry system. The present invention involves the detection of an interaction between molecules on a real-time basis through adjustment of a microreaction between traces of natural material or synthesized new drug candidates and a target material (protein or cell, etc.), thus developing materials for new drug candidates at a lower cost and with high efficiency, while improving quality of life and reducing medical costs. The present invention can be valuably used in increasing new scientific technology through the convergence of nanotechnology, biotechnology, and analytical chemistry technology. | 2011-10-20 |
20110256581 | CELL SEPARATION TECHNIQUE - In a first aspect, the present invention provides a method of removing non-viable cells from a cell population, said method comprising contacting a cell population with a compound under conditions suitable to permit binding between the compound and any non-viable cells present in the cell culture and removing at least some of the compound from said cell population. The invention further provides compositions suitable for use in these methods, further uses of such compositions and various other systems and kits. | 2011-10-20 |
20110256582 | METHOD OF CLASSIFYING HUMAN SUBJECTS HAVING ADOLESCENT IDIOPATHIC SCOLIOSIS (AIS) AND METHOD FOR SCREENING FOR A COMPOUND USEFUL IN THE TREATMENT OF AIS AND RELATED SYNDROMES CAUSING SPINAL DEFORMITIES - A method of classifying a human subject having adolescent idiopathic scoliosis (AIS) comprising: providing a cell sample isolated from the subject; detecting an impairment in melatonin-signaling pathway in the sample in the presence and in the absence of a known melatonin-signaling pathway agonist, whereby the results of the detecting step enables the classification of the subject having AIS in one AIS subgroup; and a method of screening for a compound useful in the treatment of a disease characterized by a dysfunctional melatonin-signaling pathway, said method comprising the steps of contacting a candidate compound with at least one cell expressing at least one melatonin-signaling pathway impairment, wherein the candidate compound is selected if said melatonin-signaling pathway impairment is reduced in the presence of the candidate compound as compared to that in the absence thereof. | 2011-10-20 |
20110256583 | METHOD AND MEDIUM FOR ACCELERATING TARGET ANALYTE GROWTH IN A TEST SAMPLE - A reagent mixture and testing procedure for accelerating mixing of the formulation with a specimen sample being tested by effervescing when hydrolized, thereby causing an internal “stirring” of the reagent and the specimen sample. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, environmental swabs, foodstuffs, and water, to mention a few. | 2011-10-20 |
20110256584 | CULTURE MEDIUM FOR CULTIVATION AND IDENTIFICATION OF BACTERIA OF GENUS PECTINATUS AND METHOD FOR TAKING SWAB SAMPLES - The present disclosure relates to a culture medium for cultivation and identification of bacteria of genus | 2011-10-20 |
20110256585 | MUTANT PENICILLIN G ACYLASES - The present invention relates to a mutant prokaryotic penicillin G acylase derived from a wild-type penicillin G acylase characterized in that the mutant is having an amino acid substitution at one or more amino acid positions selected from the group consisting of amino acid positions A3, A77, A90, A144 A192, B24, B109, B148, B313, B460 and B488 according to the amino acid numbering of the | 2011-10-20 |
20110256586 | Methods and Compositions for the Prevention and Treatment of Anemia - Methods for increasing and maintaining hematocrit in a mammal comprising administering a hyperglycosylated analog of erythropoietin are disclosed. An analog may be administered less frequently than an equivalent molar amount of recombinant human erythropoietin to obtain a comparable target hematocrit and treat anemia. Alternatively, a lower molar amount of a hyperglycosylated analog may be administered to obtain a comparable target hematocrit and treat anemia. Also disclosed are new hyperglycosylated erythopoietin analogs, methods of production of the analogs, and compositions comprising the analogs. | 2011-10-20 |
20110256587 | HIGH AFFINITY HUMAN ANTIBODIES TO HUMAN NERVE GROWTH FACTOR - A human antibody or antigen-binding fragment of an antibody which specifically binds human nerve growth factor (NGF) with K | 2011-10-20 |
20110256588 | METHOD OF MANUFACTURING REBAUDIOSIDE A IN HIGH YIELD BY RECYCLING BY-PRODUCTS PRODUCED FROM MANUFACTURING PROCESS FOR REBAUDIOSIDE A - The present invention relates to a method of producing Rebaudioside A in a high yield by recycling by-products produced when Rebaudioside A is produced from leaves of | 2011-10-20 |
20110256589 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 2011-10-20 |
20110256590 | BIOCHIP, REACTOR, AND REACTION METHOD - A biochip includes: a chamber that has a longitudinal direction; a holding unit that holds a liquid sample within a predetermined area of the chamber provided along the longitudinal direction, and releases the liquid sample from the predetermined area to an area inside the chamber by using a predetermined pressing force; and a pressed member that applies the predetermined pressing force to the liquid sample. | 2011-10-20 |
20110256591 | INDIVIDUALLY SYNTHESIZED PRIMERS TO BE USED IN WHOLE GENOME AMPLIFICATION - Disclosed are compositions and methods for random amplification of nucleic acid sequences of interest using random-G-deficient primers. Also disclosed are methods of randomly amplifying a target nucleic acid sequence using random G-deficient primers alone or in combination with random, partially random, or specific primers. | 2011-10-20 |
20110256592 | USE OF CYCLODEXTRINS TO IMPROVE THE SPECIFICITY, SENSITIVITY AND YIELD OF NUCLEIC ACID AMPLIFICATION REACTIONS - The invention is directed to methods for in vitro DNA synthesis catalysed by a DNA polymerase using cyclodextrins. The invention also relates to methods, compositions and kits comprising cyclodextrins for the amplification of a nucleic acid. The use of cyclodextrins improves the specificity, sensibility and/or yield of the amplification reaction. The invention is related more particularly to kits, compositions and methods for carrying out PCR reactions comprising a cyclodextrin. | 2011-10-20 |
20110256593 | Generation of Random Double-Strand Breaks in DNA Using Enzymes - An enzyme preparation is described that includes a non-specific nuclease and a T7 Endo I mutant in a unit ratio of less than 1:200. This enzyme preparation may be used to generate double-stranded DNA fragments of a size suitable for DNA sequencing. The ends of the fragments can be readily modified as necessary to ligate adaptors or individual nucleotides to one strand of the double-stranded DNA fragments. | 2011-10-20 |
20110256594 | REMOVAL OF NITROGEN FROM A CHLOROPHYLL OR PHEOPHYTIN CONTAINING BIOMASS - The present disclosure relates to refining a product from a biomass containing chlorophyll and/or pheophytins. In particular, a method of refining a product (such as a biofuel) from a photosynthetic organism is disclosed. The photosynthetic organism can be a naturally occurring organism or a genetically modified or altered organism. The method of refining comprises removing nitrogen to obtain the desired product. In some aspects, nitrogen is removed from a chlorophyll and/or pheophytin containing product by enzymatic degradation of chlorophyll and/or pheophytins and subsequent removal of the nitrogen | 2011-10-20 |
20110256595 | BIOFUEL PRODUCTION - Methods, enzymes, recombinant microorganism, and microbial systems are provided for converting polysaccharides, such as those derived from biomass, into suitable monosaccharides or oligosaccharides, as well as for converting suitable monosaccharides or oligosaccharides into commodity chemicals, such as biofuels. Commodity chemicals produced by the methods described herein are also provided. Commodity chemical enriched, refinery-produced petroleum products are also provided, as well as methods for producing the same. | 2011-10-20 |
20110256596 | MANUFACTURING METHOD OF OPTICALLY ACTIVE D TYPE LACTIC ACID USING BYPRODUCTS PREPARED FROM RICE POLISHING PROCESS - Disclosed is a method for manufacturing D type lactic acid wherein byproducts (e.g., rice bran and pulverized rice) obtained during a rice polishing process are saccharified by using α-amylase and amyloglucosidase and then fermented by a microorganism, | 2011-10-20 |
20110256597 | Membrane Supported Bioreactor for Conversion of Syngas Components to Liquid Products - Ethanol and other liquid products are produced by contacting syngas components such as CO or a mixture of CO | 2011-10-20 |
20110256598 | CO-METABOLISM OF FRUCTOSE AND GLUCOSE IN MICROBIAL PRODUCTION STRAINS - Recombinant microorganisms having an improved ability to co-metabolize in medium both fructose and glucose are described. The recombinant microorganisms comprise a promoter operably linked to a native or non-native nucleotide sequence that encodes a fucose:H+ symporter polypeptide. The recombinant microorganisms are useful for the production of a variety of products including glycerol and glycerol derivatives such as 1,3-propanediol and 3-hydroxypropionic acid. | 2011-10-20 |
20110256599 | ENHANCED PRODUCTION OF FATTY ACID DERIVATIVES - Genetically engineered cells and microorganisms are provided that produce fatty alcohols from the fatty acid biosynthetic pathway, as well as methods of their use. | 2011-10-20 |
20110256600 | Recombinant Microorganisms and Methods of Use Thereof - The invention provides a recombinant microorganism capable of producing one or more products by fermentation of a substrate comprising CO, wherein the microorganisms has an increased tolerance to ethanol. The invention also provides, inter alia, methods for the production of ethanol and one or more other products from a substrate comprising CO. | 2011-10-20 |
20110256601 | MODIFICATION OF HYDROGENASE ACTIVITIES IN THERMOPHILIC BACTERIA TO ENHANCE ETHANOL PRODUCTION - Bacteria consume a variety of biomass-derived substrates and produce ethanol. Hydrogenase genes have been inactivated m | 2011-10-20 |
20110256602 | Method for Biotechnological Production of a Hydrogen Carrier - The invention relates to a method for biotechnological production of a hydrogen carrier. It is proposed to culture ammonium-producing cyanobacteria in which, by increasing the nitrogenase activity and/or blocking the utilization of ammonium in the cell metabolism and/or providing an exit mechanism for ammonium via the cell membrane, the ammonium yield is increased. The ammonia generated by the cyanobacteria is made available as a hydrogen carrier. | 2011-10-20 |
20110256603 | APPARATUS AND METHOD OF USING AN AGRICULTURAL WASTE DIGESTER AND BIOGAS GENERATION SYSTEM - An agricultural waste digester and biogas generation system. The system includes a digester assembly having a cylindrical body, a hollow interior, a center axis and a plurality of wheel segments within the interior of the digester assembly. A gas conduit extends from the interior of the digester assembly to a power generation device. Also included is a water vessel containing water, and each of the plurality of wheel segments have an acruate, contoured surface area which restrict biogas movement within the digester assembly to produce induced agitation of agricultural waste. | 2011-10-20 |
20110256604 | GENERATION OF ASPOROGENOUS SOLVENTOGENIC CLOSTRIDIA - Expression of the SpoIIE gene in a solventogenic | 2011-10-20 |
20110256605 | CELLS WITH NON-NATURAL PHYSIOLOGIES DERIVED BY EXPRESSING LIGHT-POWERED PROTON PUMPS IN ONE OR MORE MEMBRANES - Methods and procedures for designing and constructing microbes with the ability to harvest light energy are described. In certain embodiments, these methods and procedures are used to construct a photosynthetic yeast based on proteorhodopsin (PR) expression. Proteorhodopsin is a light powered proton pump used by some ocean bacteria to scavenge light energy. By illuminating single yeast cells expressing PR, controlled amounts of energy can be delivered to these cells. A light-harvesting yeast is a unique bioenergetics research platform for investigating the interplay of biofuel production, cellular ATP levels, and the proton-motive-force (pmf). Also, a strain of yeast with light-boosted biomass to biofuel conversion efficiency possesses direct industrial and commercial utility. | 2011-10-20 |
20110256606 | Methods and Compositions for Increasing Toxin Production - The invention provides methods and compositions (such as for example, culture media) for culturing | 2011-10-20 |
20110256607 | HOMING ENDONUCLEASES - The present disclosure provides, in part, polypeptides having endonuclease activity, nucleic acid sequences for such a polypeptide, target sequences for the endonuclease, as well as vectors, cells, kits, methods, and uses of the same. | 2011-10-20 |
20110256608 | Modified Variant Bowman Birk Protease Inhibitors - The present invention relates to modified variant Bowman Birk Protease Inhibitor proteins (BBPIs) that comprise peptides that bind target proteins, and that are further modified to have greater protease inhibitory activity and/or be produced at greater yields than the unmodified BBPIs. The invention encompasses polynucleotide constructs and expression vectors containing polynucleotide sequences that encode the modified variant BBPIs, the transformed host cells that express and produce the modified variant BBPIs, the modified variant BBPI proteins, the compositions comprising the modified variant BBPIs, and the methods for making and using the modified variant BBPIs in personal care. | 2011-10-20 |
20110256609 | Compositions And Methods Comprising Variant Microbial Proteases - The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant subtilisins suitable for laundry cleaning applications. | 2011-10-20 |
20110256610 | Compositions And Methods Comprising Variant Microbial Proteases - The present invention provides variant subtilisins and compositions comprising at least one variant subtilisin set forth herein, as well as methods for using these variants and compositions. In some embodiments, the present invention provides variant proteases suitable for laundry cleaning applications. | 2011-10-20 |
20110256611 | INTERFERON BETA PRODUCTION PROMOTER AND A METHOD FOR PRODUCING THEREOF - An interferon β production promoter and a method for producing thereof are provided. The interferon β production promoter has a culture, a cell or cell components of lactic acid bacteria as an active ingredient thereof. RNA can be used for the cell component. In addition, bacteria belonging to the genus | 2011-10-20 |
20110256612 | COMPOSITIONS FOR CULTURING SPIROCHETES - The present invention relates to methods and compositions for culturing spirochetes and treating spirochetal diseases. For example, the present invention provides serum-free media for culturing spirochete bacteria in vitro. The present invention further provides methods for identifying spirochete susceptibilities to antimicrobials and antimicrobial compositions and cocktails. The present invention also provides methods for treating subjects suspected of having a spirochete infection. | 2011-10-20 |
20110256613 | Designer proton-channel transgenic algae for photobiological hydrogen production - A designer proton-channel transgenic alga for photobiological hydrogen production that is specifically designed for production of molecular hydrogen (H | 2011-10-20 |
20110256614 | METHOD AND SYSTEM FOR PROCESSING ORGANIC MATTER IN A POULTRY FARM - The invention provides a method and system for processing organic matter that is produced in a poultry farm. The organic matter produced by the plurality of birds is removed from one or more containers that house the plurality of birds. The method further includes treating a first amount of organic matter of the organic matter in a thermal processor to produce inorganic matter. Thereafter, the inorganic matter and a second amount of organic matter of the organic matter are consumed in one or more photo bioreactors for growing one or more photosynthesis organisms. | 2011-10-20 |
20110256615 | MODIFICATION OF BIOMASS FOR EFFICIENT CONVERSION TO FUELS - A process is disclosed for preparing biomass particles for thermolytic or enzymatic conversion whereby the biomass particles baying a moisture content of at least 20% are subjected to flash heating. The flash heating may be preceded by one or more adsorption/desorption cycles with water or steam. A swelling aid may be added during the adsorption part of an adsorption/desorption cycle. | 2011-10-20 |
20110256616 | Cooling in a Thermal Cycler Using Heat Pipes - A device for amplifying a nucleic acid sample may include a sample holder configured to receive a nucleic acid sample, a heating system configured to raise the temperature of the sample, a cooling system configured to lower the temperature of the sample, and a controller configured to operably control the heating system and the cooling system to cycle the device through a desired time-temperature profile. The cooling system may include at least one heat pipe and a heat sink and the at least one heat pipe may include a first portion disposed proximate to the sample holder and a second portion disposed proximate to the heat sink. | 2011-10-20 |
20110256617 | DEVICE FOR DETECTING THE BACTERIAL CHARGE IN A LIQUID OR SEMI-LIQUID FOOD PRODUCT | 2011-10-20 |
20110256618 | Nucleic acid sequencing methods and systems - Sequencing methods that use an exonuclease that comprises template dependent nucleobase binding activity are provided. Related compositions and sequencing systems are also provided. | 2011-10-20 |
20110256619 | MICROFABRICATED COMPOSITIONS AND PROCESSES FOR ENGINEERING TISSUES CONTAINING MULTIPLE CELL TYPES - The present invention relates to a three-dimensional system, and compositions obtained therefrom, wherein individual layers of the system comprise channels divided longitudinally into two compartments by a centrally positioned membrane, and wherein each compartment can comprise a different cell type. | 2011-10-20 |
20110256620 | NUCLEIC ACID ENCODING A SELF-ASSEMBLING SPLIT-FLUORESCENT PROTEIN SYSTEM - The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from | 2011-10-20 |
20110256621 | COLLOIDAL MAGNETIC NANOBIOPARTICLES FOR CYTOTOXICITY AND DRUG DELIVERY - The present invention provides systems that include magnetic colloidal particles of differing elemental compositions with differing morphologies. The colloidal particles comprise a magnetic material and a shell that surrounds the magnetic material. The colloidal particles can be inductively heated using a magnetic field. The present invention also provides applications of such colloidal particles, such as methods for cytotoxicity and for drug delivery. | 2011-10-20 |
20110256622 | Novel Culture Systems for Ex Vivo Development - The present invention provides methods for the culture of animal pluripotent stem cells and their differentiated progeny cells, tissues, and organs, and nonhuman animal embryos and fetuses. | 2011-10-20 |
20110256623 | Primate Embryonic Stem Cells - A purified preparation of primate embryonic stem cells is disclosed. This preparation is characterized by the following cell surface markers: SSEA-1 (−); SSEA-4 (+); TRA-1-60 (+); TRA-1-81 (+); and alkaline phosphatase (+). In a particularly advantageous embodiment, the cells of the preparation are human embryonic stem cells, have normal karyotypes, and continue to proliferate in an undifferentiated state after continuous culture for eleven months. The embryonic stem cell lines also retain the ability, throughout the culture, to form trophoblast and to differentiate into all tissues derived from all three embryonic germ layers (endoderm, mesoderm and ectoderm). A method for isolating a primate embryonic stem cell line is also disclosed. | 2011-10-20 |
20110256624 | BIOREACTOR - The invention relates to a bioreactor, to the use of the bioreactor for culturing microorganisms or cell cultures, and also to a method for culturing microorganisms or cell cultures. | 2011-10-20 |
20110256625 | Liver Engrafting Cells, Assays, and Uses Thereof - A substantially enriched mammalian hepatic liver engrafting cell population is provided. Methods are provided for the isolation and culture of this liver engrafting cell. The progenitor cells are obtained from a variety of sources, including fetal and adult tissues. The cells are useful in transplantation, for experimental evaluation, and as a source of lineage and cell specific products, including mRNA species useful in identifying genes specifically expressed in these cells, and as targets for the discovery of factors or molecules that can affect them. | 2011-10-20 |
20110256626 | METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS WITH HIGH EFFICIENCY AND INDUCED POLURIPOTENT STEM CELLS PROUCED THEREBY - The present invention provides a method for producing customized pluripotent stem cells. Specifically, the present invention comprises following steps: extracting proteins from any of the dedifferentiated stem cells or induced pluripotent stem cells, the said dedifferentiated or pluripotent stem cells being prepared by any known method; introducing the protein extract into the adult somatic cells; and culturing the adult somatic cells to produce pluripotent stem cells having the same pluripotency as that of embryonic stem cells. In addition, pluripotent stem cells produced according to the present method and cell therapeutics comprising the same are provided. The method allows pluripotent stem cells to be produced very easily and at a significantly higher yield, compared to typical methods. | 2011-10-20 |
20110256627 | Cutting device for culturing the next generation of cells - The present invention is related to a device for subculturing embryonic stem cells, which mainly contains a cutting brush having a handle and a brush stem. The brush stem has a stem and a plurality of discrete upright wires disposed on the stem. The brush stem is mounted onto a front end of the handle, and an angle between the brush stem and the handle ranges from 70 to 90 degrees. The distance between two adjacent wires is smaller than the diameter of a block of the embryonic stem cells to be cut for next generation culturing, so that the cutting brush can evenly cut the block into a plurality of smaller blocks of embryonic stem cells. | 2011-10-20 |
20110256628 | ADAPTIVE TISSUE ENGINEERING SCAFFOLD - The embodiments described herein include porous scaffolds formed from a stimuli-responsive polymer. The stimuli-responsive polymer of the scaffold creates a “smart” scaffold that changes properties in response to an effective stimulus applied to the stimuli-responsive polymer. In a preferred embodiment, an effective stimulus applied to the scaffold initiates a phase transition event in the stimuli-responsive polymer that results in a change in the volume of the pores of the scaffold. The scaffolds can be used to capture appropriately sized objects (e.g., cells) by using the volume-change properties of the pores. Relatedly, the scaffolds can be used as tissue-engineering scaffolds by capturing cells in the pores and introducing the cell-loaded scaffold into a cell-growth environment (e.g., in vivo). | 2011-10-20 |
20110256629 | FULLY AUTOMATIC BIOCHEMICAL ANALYZER AND ANALYZING METHOD THEREOF - A fully automatic biochemical analyzer and an analyzing method thereof are provided, in which the fully automatic biochemical analyzer includes two concentric reagent trays and two driving systems. The two driving systems respectively drive the two reagent trays to rotate, so the two reagent trays are capable of independent rotation, thereby avoiding various drawbacks resulting from required simultaneous rotation and stopping of the two reagent trays. The two reagent trays are concentrically disposed. Under a particular limitation of the table size, more reagent positions can be disposed, thereby increasing the number of items that can be analyzed at the same time by the analyzer. | 2011-10-20 |
20110256630 | ASSAY APPARATUSES, METHODS AND REAGENTS - We describe apparatuses, systems, method, reagents, and kits for conducting assays as well as process for their preparation. They are particularly well suited for conducting automated sampling, sample preparation, and analysis in a multi-well plate assay format. For example, they may be used for automated analysis of particulates in air and/or liquid samples derived therefrom in environmental monitoring. | 2011-10-20 |
20110256631 | METHODS AND PROCESSES FOR CALLING BASES IN SEQUENCE BY INCORPORATION METHODS - Computer implemented methods, and systems performing such methods for processing signal data from analytical operations and systems, and particularly in processing signal data from sequence-by-incorporation processes to identify nucleotide sequences of template nucleic acids and larger nucleic acid molecules, e.g., genomes or fragments thereof. | 2011-10-20 |
20110256632 | Additive for Polyolefin Polymerization Processes - A polymerization process is disclosed, including: polymerizing at least one olefin to form an olefin based polymer in a polymerization reactor; and feeding at least one ethyleneimine additive to the polymerization reactor. The ethyleneimine additive may comprise a polyethyleneimine, an ethyleneimine copolymer, or a mixture thereof. The process may further comprise monitoring static in the polymerization reactor; maintaining the static at a desired level by use of at least one ethyleneimine additive, the at least one ethyleneimine additive present in said reactor in the range from about 0.1 to about 50 ppm, based on the weight of polymer produced by said combining. | 2011-10-20 |
20110256633 | Secretory Granules and Granulogenic Factors as a Target for Cancer Treatment - The present invention relates to a method for screening a cancer therapeutic agent, comprising the steps of: (a) contacting a test substance of interest with a cell containing a granulogenic factor-encoding nucleotide sequence; and (b) analyzing expression of the granulogenic factor or production of secretory granules, wherein the test substance is determined as the cancer therapeutic agent where it inhibits the expression of the granulogenic factor or the production of secretory granules. In the present invention, the expression of the granulogenic factor contributes to induction of secretory granule formation in non-secretory cells, and inhibition of the granulogenic factor expression leads to inhibition of secretory granule formation in secretory cells. In addition, the secretory granules produced by the present granulogenic factor change cell activities via the IP | 2011-10-20 |
20110256634 | SENSOR MEASURING METHOD AND SENSING APPARATUS - A method of performing a measurement with a sensor having a sensing surface and at least one capture molecule attached to the sensing surface for forming a binding pair with an analyte of interest, the binding pair having a flexible spatial orientation, the method comprising capturing the analyte of interest with the capture molecule, thereby forming the binding pair in an initial spatial orientation; applying a first electromagnetic force to the sensing surface to alter the spatial orientation of the binding pair; and performing a sensor measurement with the binding pair in the altered spatial orientation. A sensor apparatus implementing this method is also disclosed. | 2011-10-20 |
20110256635 | SOLUBLE HUMAN ST-2 ANTIBODIES AND ASSAYS - Provided herein are antibodies and antigen-binding antibody fragments that bind to human soluble Growth Stimulation-Expressed Gene 2 (ST2) protein, kits containing these antibodies and antibody fragments, and methods of using these antibodies and antibody fragments. | 2011-10-20 |
20110256636 | METHODS AND REAGENTS FOR DIAGNOSING RHEUMATOID ARTHRTIS - Methods for diagnosing rheumatoid arthritis (RA) are disclosed, using measurement of the CCL8 protein level in a test sample from a subject. Testing for CCL8 as an indicator of RA can be combined with testing for other indicators of RA, including clinical assessments, imaging or other RA markers such as Rheumatoid factor (RF). CCL8 testing can be used for discriminating RA from other diseases or conditions, evaluating the severity of RA. Related diagnostic reagents, kits, pharmaceutical compositions, and methods for identifying a candidate substance as a therapeutic agent for treating rheumatoid arthritis are also described. | 2011-10-20 |
20110256637 | Target Detection Using a Single-Stranded, Self-Complementary, Triple-Stem DNA Probe - Provided are novel single-stranded oligonucleotide probes that have a triple-stem configuration in the absence of target binding to the target binding sequence. The probes also have a fluorophore and a quencher. In the absence of target binding to the target binding sequence, these single-stranded oligonucleotide probes are capable of forming self-complementary duplexes such that the probe is in the triple-stem configuration and the fluorophore is positioned adjacent the quencher. In the presence of target binding to the target binding sequence, formation of the self-complementary duplexes is inhibited such that the probe is configured to position the fluorophore away from the quencher such that a signal of the fluorophore is detectable. Also provided are methods of using the probes. | 2011-10-20 |
20110256638 | DOWNWARD OR VERTICAL FLOW DIAGNOSTIC DEVICE AND ASSAY - A downward or vertical flow-through rapid diagnostic device and assay are provided. The device comprises a test area and reagent storage area, which are linked via a channel. The test area further comprises a reaction zone and an absorbent zone. A capture reagent is immobilized on the reaction zone to detect a target analyte in the fluid test sample. The fluid test sample flows downward or vertically through the reaction zone and into the absorbent zone, with the capture reagent and target analyte forming a two-membered complex that is concentrated in the reaction zone. The reagent storage area comprises a breakable cartridge positioned directly and vertically above the test area and a channel. A reagent used in the assay is housed in the breakable cartridge. Once liberated, the reagent passes through the channel and flows to test area for depositing on the reaction zone. The storage of predetermined amounts of reagents in the diagnostic device reduces the number of manual operations required to produce a result. | 2011-10-20 |