39th week of 2018 patent applcation highlights part 30 |
Patent application number | Title | Published |
20180273914 | DEVICE AND METHOD OF COLLECTION FOR RNA VIRUSES - The present disclosure generally relates to a method and device for inactivation and dry storage, under ambient conditions, of a biological sample containing RNA virus. Methods for collecting and recovering RNA from a biological sample and subsequent analysis for a virus are also provided. | 2018-09-27 |
20180273915 | Recombinant Host Cells For The Production Of 3-Hydroxypropionic Acid - Provided herein are recombinant host cells having an active 3-Hydroxypropionic Acid (3-HP) pathway wherein the host cells comprise a heterologous polynucleotide encoding a 3-hydroxypropionate dehydrogenase (3-HPDH). Also described are methods of using the recombinant cells to produce 3-HP and derivatives of 3-HP (e.g., acrylic acid). | 2018-09-27 |
20180273916 | NOVEL 7BETA-HYDROXYSTEROID DEHYDROGENASES AND THEIR USE - The invention relates to novel 7ß-hydroxysteroid dehydrogenases which are obtainable from bacteria of the genus | 2018-09-27 |
20180273917 | HEME PROTEIN CATALYSTS FOR CARBON-BORON BOND FORMATION IN VITRO AND IN VIVO - Provided herein are methods for producing an organoboron product. The methods include combining a boron-containing reagent and a carbene precursor in the presence of a heme protein, e.g., a cytochrome c, a cytochrome P450, a globin, a protoglobin, a nitric oxide dioxygenase, a peroxidase, or a catalase, or a variant thereof, under conditions sufficient to form the organoboron product. Reaction mixtures for producing organoboron products are also described, as well as whole-cell catalysts comprising heme proteins and variants thereof for forming carbon-boron bonds. | 2018-09-27 |
20180273918 | GENES AND USES THEREOF, METHODS FOR SYNTHESIZING ODD NUMBERED MEDIUM CHAIN ALIPHATIC ALDEHYDES AND METHODS FOR SYNTHESIZING EVEN NUMBERED MEDIUM CHAIN ALIPHATIC HYDROCARBONS - Provided are genes, coding proteins and uses thereof, gene elements, genes and uses, gene elements, methods for synthesizing odd numbered medium chain aliphatic aldehydes, for synthesizing odd numbered medium chain aliphatic alcohol and for synthesizing even numbered medium chain aliphatic hydrocarbons. Provided is method for producing odd numbered aliphatic alcohols in | 2018-09-27 |
20180273919 | GLUCOSYLTRANSFERASE ENZYMES FOR PRODUCTION OF GLUCAN POLYMERS - Compositions are disclosed herein comprising poly alpha-1,3-1,6-glucan with a weight average degree of polymerization (DP | 2018-09-27 |
20180273920 | DEIMMUNIZED THERAPEUTIC COMPOSITIONS AND METHODS - This disclosure provides a bispecific ligand directed toxin (BLT) that includes a diphtheria toxin (DT) molecule that has been mutated to create a DT molecule that induces less of an immune response than native diphtheria toxin. The deimmunized DT molecule is fused with targeting ligands to create a fusion protein that can selectively deliver the deimmunized DT to target cells to kill the target cells. | 2018-09-27 |
20180273921 | Polypeptide Containing DNA-Binding Domain - The present invention provides an artificial nuclease comprising a DNA-binding domain and a function domain linked to each other via a polypeptide consisting of 35 to 55 amino acid residues wherein amino acid residues at two sites in a DNA-binding module contained in a DNA-binding domain exhibit a mode of repetition that is different for every four DNA-binding modules; a vector for expressing said artificial nuclease; a vector library for preparing said vector; and a vector set for preparing said vector library. | 2018-09-27 |
20180273922 | Methods and Compositions for Modifying a Single Stranded Target Nucleic Acid - The present disclosure provides compositions and methods for binding and/or cleaving a single stranded target nucleic acid. Subject compositions include a Cas9 polypeptide, a guide nucleic acid, and a PAMmer. A subject PAMmer is a single stranded oligonucleotide having a proto spacer adjacent motif (PAM) sequence and at least one of: a specifity segment positioned 5′ of the PAM sequence, and an orientation segment positioned 3′ of the PAM sequence. In some embodiments, the Cas9 polypeptide is a variant Cas9 polypeptide having reduced nuclease activity relative to a corresponding wild type Cas9 polypeptide. In some cases, methods of binding are for visualizing single stranded target nucleic acids using a detectable label. In some cases, methods of binding are for isolating, collecting, and/or analyzing at least one of: (i) bound single stranded target nucleic acids; and (ii) polypeptides associated with bound single stranded target nucleic acids. | 2018-09-27 |
20180273923 | TAL EFFECTOR MEANS USEFUL FOR PARTIAL OR FULL DELETION OF DNA TANDEM REPEATS - The application relates to means, which derive from TAL effectors and TALENs. The structure of the means of the application is especially adapted for partial or full deletion of at least one DNA tandem repeat, more particularly for partial or full deletion of at least one DNA tandem repeat in a double-stranded DNA, more particularly for partial or full deletion of at least one DNA tandem repeat, which is contained in a double-stranded DNA and, which forms a complex secondary structure, such as a hairpin, a triple helix or a tetraplex secondary structure. The means of the application are notably useful in the treatment and/or prevention and/or palliation of a disease or disorder involving at least one DNA tandem repeat, such as DM1, SCA8, SCA12, HDL2, SBMA, HD, DRPLA, SCA1, SCA2, SCA3, SCA6, SCA7, SCA17, PSACH, DM2, SCA10, SPD1, OPMD, CCD, HPE5, HFG syndrome, BPES, EIEE1, FRAXA, FXTAS and FRAXE. | 2018-09-27 |
20180273924 | POLYPEPTIDES HAVING ALPHA-AMYLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to isolated polypeptides having alpha-amylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains. | 2018-09-27 |
20180273925 | ALPHA-AMYLASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to alpha-amylase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2018-09-27 |
20180273926 | MULTIFUNCTIONAL MICROBIAL CELLULASES - Disclosed herein are methods of making multifunctional microbial cellulases. The engineered multifunctional microbial cellulases disclosed herein exhibit improved activity over native cellulases. | 2018-09-27 |
20180273927 | Polypeptides Having Protease Activity - The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, in particular the use of the proteases in animal feed. | 2018-09-27 |
20180273928 | RECOMBINANT ELASTASE PROTEINS AND METHODS OF MANUFACTURING AND USE THEREOF - The present invention relates to methods for the manufacture, purification, formulation, and use of biologically active recombinant elastase proteins. Described are recombinant methods for producing therapeutically useful elastase proteins, as are pharmaceutical compositions comprising said elastase proteins. Novel recombinant elastase proteins and protein preparations are also disclosed. Methods are described for treating and preventing diseases of biological conduits using pharmaceutical compositions containing the elastase proteins of the invention. | 2018-09-27 |
20180273929 | ASPARAGINASE - The present invention relates to a polypeptide having asparaginase activity selected from the group consisting of: (i) a polypeptide having an amino acid sequence comprising the mature polypeptide sequence of SEQ ID NO: 1; (ii) a polypeptide comprising an amino acid sequence that has at least 50% sequence identity with the mature polypeptide sequence of SEQ ID NO: 1; (iii) a polypeptide encoded by a nucleic acid comprising a sequence that hybridizes under medium stringency conditions to the complementary strand of the mature polypeptide encoding sequence of SEQ ID NO: 2; and (iv) a polypeptide comprising an amino acid sequence encoded by a nucleic acid that has at least 50% sequence identity to the mature polypeptide coding sequence of SEQ ID NO: 2. The polypeptide may be used in the preparation of a food product. | 2018-09-27 |
20180273930 | Producing Adipic Acid and Related Compounds Using Hybrid Polyketide Synthases - The present invention provides for a polyketide synthase (PKS) capable of synthesizing a carboxylic acid, said PKS comprising a synthetic or hybrid module. The present invention also provides for a host cell comprising the PKS and when cultured produces the carboxylic acid. In some embodiments, the carboxylic acid is adipic acid. | 2018-09-27 |
20180273931 | CRYSTALLINE MATERIALS ON BIOLOGICAL TISSUE AND METHODS FOR MAKING THE SAME - Provided are compositions including a direct interface between a biological tissue and a crystalline material, wherein the crystalline material has a crystallization temperature that exceeds the temperature at which the biological tissue incurs thermal damage. Also provided are methods for producing said compositions. | 2018-09-27 |
20180273932 | USE OF EXONUCLEASES TO IMPROVE CRISPR/CAS-MEDIATED GENOME EDITING - The present disclosure is directed to methods of producing a modified nucleic acid comprising a precise deletion in a target nucleic acid in a cell comprising generating, within the cell, a first single strand break on a first strand of the target nucleic acid and a second single strand break on a second strand of the target nucleic acid, thereby forming a double strand break in the target nucleic acid having a first 3′ overhang and a second 3′ overhang; processing the first 3′ overhang and the second 3′ overhang with an exonuclease molecule, thereby deleting the segment of the target nucleic acid that was located between the first single strand break and the second single strand break, and forming a processed double strand break; and allowing the processed double strand break to be repaired by at least one DNA repair pathway, thereby producing the modified nucleic acid comprising the precise deletion in the target nucleic acid in the cell. Gene editing systems, vectors, polynucleotides, and methods of treatment are also disclosed herein. | 2018-09-27 |
20180273933 | METHODS AND COMPOSITIONS FOR ANALYZING CELLULAR COMPONENTS - Embodiments of the present invention relate to analyzing components of a cell. In some embodiments, the present invention relate to analyzing components of a single cell. In some embodiments, the methods and compositions relate to sequencing nucleic acids. In some embodiments, the methods and compositions relate to identifying and/or quantitating nucleic acid, proteins, organelles, and/or cellular metabolites. | 2018-09-27 |
20180273934 | SELECTIVE OPTIMIZATION OF A RIBOSOME BINDING SITE FOR PROTEIN PRODUCTION - A method for optimising a nucleotide sequence for protein expression in a host cell, comprising the steps of: (a) constructing an expression library comprising a large number of variants of the sequence to be expressed operatively cloned in an expression vector, wherein (i) the sequence of the 6 nucleotides immediately upstream (5′-direction) of the first codon of the coding sequence to be expressed is completely or partially randomized; (ii) the sequence of the second and third codons of the coding sequence to be expressed is randomized, wherein the randomization of the second and third codons is limited to changes not altering the amino-acids encoded by said codons; (b) screening the library with regard to efficiency of protein expression in the host cell; and (c) selecting a sequence resulting in a desired level of efficiency of protein expression. | 2018-09-27 |
20180273935 | METHODS AND COMPOSITIONS FOR GENERATING CRISPR/CAS GUIDE RNAS - The present disclosure provides methods, kits, and compositions for generating DNA molecules encoding CRISPR/Cas guide RNAs (e.g., Cas9 single guide RNAs or Cas9 targeter RNAs). A library of such DNA molecules can be generated from any DNA source. The methods include a step of contacting target DNA with one or more DNA endonucleases that specifically bind to and cleave within a recognition sequence that includes a PAM sequence, to generate a plurality of cleavage fragments, to which a DNA adapter can be attached. A distal-cleaving DNA endonuclease can be used that specifically binds to a recognition sequence in the DNA adapter and cleaves at a site within the attached DNA cleavage fragments to generate a library of CRISPR/Cas guide sequences. After removal of all or a portion of the DNA adapter, a constant region of a guide RNA can be attached to generate DNA molecules encoding CRISPR/Cas guide RNAs. | 2018-09-27 |
20180273936 | VARIANT LIBRARIES OF THE IMMUNOLOGICAL SYNAPSE AND SYNTHESIS THEREOF - Disclosed herein are methods for the generation of highly accurate nucleic acid libraries encoding for predetermined variants of a nucleic acid sequence. The nucleic acid sequence may encode for all or part of a reference domain of a CAR. The degree of variation may be complete, resulting in a saturated variant library, or less than complete, resulting in a non-saturating library of variants. The variant nucleic acid libraries described herein may be designed for further processing by transcription or translation. The variant nucleic acid libraries described herein may be designed to generate variant RNA, DNA and/or protein populations. Further provided herein are method for identifying variant species with increased or decreased activities, with applications in regulating biological functions and the design of therapeutics for treatment or reduction of a disease, such as cancer. | 2018-09-27 |
20180273937 | METHODS AND COMPOSITIONS FOR SEQUENCE SPECIFIC ANTIMICROBIALS - The invention relates to antimicrobial compositions comprising cell-penetrating peptides linked to CRISPR RNAs and methods for their use. | 2018-09-27 |
20180273938 | GENOME EDITING ENHANCEMENT - This invention pertains to single-stranded carrier nucleic acids and their methods of use for enhancing genome editing ribonucleoprotein complex transfection into cells and the resulting enhancement of CRISPR editing on the target DNA within those cells, as well as introduction of chemical modifications which reduce the integration of the single-stranded carrier nucleic acids at double-stranded breaks. | 2018-09-27 |
20180273939 | sgRNA and method for specifically activating human RSPO2 gene with CRISPR-Cas9 and application thereof - A method of constructing a specific CRISPR-Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats associated) to activate a RSPO2 (R-spondin 2) gene is disclosed in the present invention. The method comprises the following steps: designing a sgRNA (single guide RNA) of a specifically targeted human RSPO2 gene; constructing a CRISPR-Cas9 recombinant lentivirus vector of a specifically activated RSPO2 gene; and lentiviral packaging a CRISPR-Cas9 system of the specifically activated RSPO2 gene. The CRISPR-Cas9 system designed by the present invention activates the RSPO2 target gene expression and promotes the activation of the hepatic stellate cell. | 2018-09-27 |
20180273940 | TREATING AND PREVENTING MICROBIAL INFECTIONS - The invention provides methods for treating or preventing microbial (eg, bacterial) infections and means for performing these methods. In particular, treatment of infections requiring rapid and durable therapy is made possible, such as for treating acute conditions such as septicemia, sepsis, SIRS or septic shock. The invention is particularly useful, for example, for treatment of microbes such as for environmental, food and beverage use. The invention relates inter alia to methods of controlling microbiologically influenced corrosion (MIC) or biofouling of a substrate or fluid in an industrial or domestic system. The invention also useful for the treatment of pathogenic bacterial infections in subjects receiving a treatment for a disease or condition, such as a transplant or a treatment for cancer, a viral infection or an autoimmune disease. | 2018-09-27 |
20180273941 | Short Non-Coding Protein Regulatory RNAs (sprRNAs) and Methods of Use - The present invention provides isolated nucleic acid molecules comprising short non-coding protein regulatory RNAs (sprRNA), variants, fragments and inhibitors thereof and compositions and methods of using the same. | 2018-09-27 |
20180273942 | COMPOUNDS AND METHODS FOR MODULATION OF DUX4 - The present disclosure pertains generally to double-stranded small interfering RNAs that modulate gene expression for use in research, diagnostics, and/or therapeutics. In certain embodiments, the present disclosure provides double-stranded small interfering RNAs that modulate DUX4 gene expression. In certain embodiments, the present disclosure provides methods of inhibiting DUX4 gene expression by contacting a cell with double-stranded small interfering RNAs. | 2018-09-27 |
20180273943 | MODULATION OF SMN EXPRESSION - Certain embodiments are directed to methods and compounds for inhibiting SMN-NAT, the natural antisense transcript of SMN. Such methods and compounds are useful for increasing expression of SMN in cells and animals | 2018-09-27 |
20180273944 | OLIGONUCLEOTIDE-BASED INHIBITORS COMPRISING LOCKED NUCLEIC ACID MOTIF - The present invention relates to chemical modification motifs for oligonucleotides. The oligonucleotides of the present invention, such as chemically modified antisense oligonucleotides, can have increased in vivo efficacy. The chemically modified oligonucleotides provide advantages in one or more of potency, efficiency of delivery, target specificity, toxicity, and/or stability. The chemically modified oligonucleotides have a specific chemical modification motif or pattern of locked nucleic acids (LNAs). The oligonucleotide (e.g. antisense oligonucleotide) can target RNA, such as miRNA or mRNA. Also provided herein are compositions comprising the chemically modified oligonucleotides and methods of using the chemically modified oligonucleotides as therapeutics for various disorders, including cardiovascular disorders. | 2018-09-27 |
20180273945 | COMPOSITIONS AND THEIR USES DIRECTED TO HUNTINGTIN - Disclosed herein are compounds, compositions and methods for modulating the expression of huntingtin in a cell, tissue or animal. Further provided are methods of slowing or preventing Huntington's disease progression using an antisense compound targeted to huntingtin. Additionally provided are methods of delaying or preventing the onset of Huntingtin's disease in an individual susceptible to Huntingtin's Disease. Also provided are uses of disclosed compounds and compositions in the manufacture of a medicament for treatment of diseases and disorders. | 2018-09-27 |
20180273946 | MODULATION OF TRANSTHYRETIN EXPRESSION - Provided herein are methods, compounds, and compositions for reducing expression of transthyretin mRNA and protein in an animal. Such methods, compounds, and compositions are useful to treat, prevent, delay, or ameliorate transthyretin amyloidosis, or a symptom thereof. | 2018-09-27 |
20180273947 | PROGRAMMED CELL DEATH 1 LIGAND 1 (PD-L1) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The present invention relates to RNAi agents, e.g., double stranded RNAi agents, targeting the programmed cell death 1 ligand 1 (PD-L1) gene, and methods of using such RNAi agents to inhibit expression of a PD-L1 gene and methods of treating subjects having a PD-L1-associated disorder. | 2018-09-27 |
20180273948 | RNAi CONJUGATES, PARTICLES AND FORMULATIONS THEREOF - Particles, including nanoparticles and microparticles, and pharmaceutical formulations thereof, comprising conjugates of an RNAi agent attached to a targeting moiety via a linker have been designed which can provide improved temporospatial delivery of the RNAi agent and/or improved biodistribution. Methods of making the conjugates, the particles, and the formulations thereof are provided. Methods of administering the formulations to a subject in need thereof are provided, for example, to treat or prevent cancer or infectious diseases. | 2018-09-27 |
20180273949 | MODULATION OF TRANSTHYRETIN EXPRESSION - Compounds, compositions and methods are provided for modulating the expression of transthyretin. The compositions comprise oligonucleotides, targeted to nucleic acid encoding transthyretin. Methods of using these compounds for modulation of transthyretin expression and for diagnosis and treatment of diseases and conditions associated with expression of transthyretin are provided. | 2018-09-27 |
20180273950 | METHODS AND COMPOSITIONS FOR THE PRODUCTION OF SIRNAS - The technology described herein relates to siRNAs, e.g., methods and compositions relating to the production of siRNAs in bacterial cells. | 2018-09-27 |
20180273951 | METHODS FOR MODULATING KLHL1 LEVELS, METHODS FOR MODULATING CURRENT ACTIVITY IN T-TYPE CALCIUM CHANNELS, MOLECULES THEREFOR, AND METHODS FOR IDENTIFYING MOLECULES THEREFOR - Methods for modulating T-type calcium channel activity without directly targeting the T-type calcium channels are provided that include modulating kelch-like protein 1 (KLHL1) levels in a subject by providing a small hairpin RNA (shRNA) that targets a KLHL1 gene, and then administering the shRNA to the subject in an amount sufficient to modulate KLHL1 gene expression. The KLHL1 level directly effects current activity in T-type calcium channels and therefore modulation of KLHL1 gene expression indirectly modulates current activity in T-type calcium channels. The methods may be implemented with, for example, an shRNA molecule suitable for modulating a KLHL1 level in the subject which may be provided as a plasmid encoding the shRNA molecule or an adeno-associated virus vector encoding the shRNA molecule. Methods of identifying compounds that modulate current activity in T-type calcium channels by determining an effect of the compound on KLHL1 gene expression are also provided. | 2018-09-27 |
20180273952 | COMPOSITIONS AND METHODS FOR MODULATING APOLIPOPROTEIN C-III EXPRESSION - Provided herein are oligomeric compounds with conjugate groups targeting apolipoprotein C-III (ApoCIII). In certain embodiments, the ApoCIII targeting oligomeric compounds are conjugated to N-Acetylgalactosamine. Also disclosed herein are conjugated oligomeric compounds targeting ApoCIII for use in decreasing ApoCIII to treat, prevent, or ameliorate diseases, disorders or conditions related to ApoCIII. Certain diseases, disorders or conditions related to ApoCIII include inflammatory, cardiovascular and/or metabolic diseases, disorders or conditions. The conjugated oligomeric compounds disclosed herein can be used to treat such diseases, disorders or conditions in an individual in need thereof. | 2018-09-27 |
20180273953 | COMPOSITIONS AND METHODS FOR MODULATING APOLIPOPROTEIN (a) EXPRESSION - Provided herein are oligomeric compounds with conjugate groups targeting apoplipoprotein (a) [apo(a)]. In certain embodiments, the apo(a) targeting oligomeric compounds are conjugated to N-Acetylgalactosamine. Also disclosed herein are conjugated oligomeric compounds targeting apo(a) for use in decreasing apo(a) to treat, prevent, or ameliorate diseases, disorders or conditions related to apo(a) and/or Lp(a). Certain diseases, disorders or conditions related to apo(a) and/or Lp(a) include inflammatory, cardiovascular and/or metabolic diseases, disorders or conditions. The conjugated oligomeric compounds disclosed herein can be used to treat such diseases, disorders or conditions in an individual in need thereof. | 2018-09-27 |
20180273954 | INDUCED EXON INCLUSION IN SPINAL MUSCLE ATROPHY - The invention relates to the use of an antisense compound for inducing exon inclusion as a treatment for Spinal Muscle Atrophy (SMA). More particularly it relates to inducing inclusion of exon 7 to restore levels of Survival Motor Neuron (SMN) protein encoded by the Survival Motor Neuron (SMN) gene. | 2018-09-27 |
20180273955 | STEROL REGULATORY ELEMENT BINDING PROTEIN (SREBP) CHAPERONE (SCAP) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to double stranded ribonucleic acid (dsRNAi) agents and compositions targeting the SCAP gene, as well as methods of inhibiting expression of a SCAP gene and methods of treating subjects having a SCAP-associated disorder, such as nonalcoholic fatty liver disease (NAFLD) or nonalcoholic steatohepatitis (NASH), using such dsRNAi agents and compositions. | 2018-09-27 |
20180273956 | Bacteria Engineered to Treat Diseases that Benefit from Reduced Gut Inflammation and/or Tighten Gut Mucosal Barrier - Genetically engineered bacteria, pharmaceutical compositions thereof, and methods of treating or preventing autoimmune disorders, inhibiting inflammatory mechanisms in the gut, and/or tightening gut mucosal barrier function are disclosed. | 2018-09-27 |
20180273957 | BIOLOGICAL INDICATOR - This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed. | 2018-09-27 |
20180273958 | BIOLOGICAL INDICATOR - This invention relates to a biological indicator derived from a composition comprising: a host organism comprising a spore forming bacteria; a reporter gene for producing an indicator enzyme; a regulatory gene; and a vehicle for inserting the reporter gene and the regulatory gene in the host organism; the host organism bearing a transposable genetic element in its genome for inserting an insertion sequence in the regulatory gene; the insertion sequence comprising a transposase, a pair of terminal inverted repeat sequences, and at least one open reading frame for expressing the transposase. The vehicle may be taken up by the host organism. The insertion sequence may be inserted in the regulatory gene. The host organism may undergo sporulation to form the biological indicator. A process and an apparatus for using the biological indicator are disclosed. | 2018-09-27 |
20180273959 | METHODS FOR PRODUCING TRANSGENIC PLANTS - A method of producing a stably transformed corn plant in a single container is demonstrated. This method allows for the automation of the transformation process and reduces labor, material, and ergonomic costs associated with traditional plant tissue culture systems. | 2018-09-27 |
20180273960 | METHODS AND COMPOSITIONS FOR MARKER-FREE GENOME MODIFICATION - Compositions and methods are provided for modifying a nucleotide sequence in the genome of a plant cell, without the use of a selectable marker. The methods and compositions employ a guide polynucleotide/Cas endonuclease system to make a double strand break in a target site located in a nucleotide sequence and plant cells are obtained without the use of a selectable marker, and to provide an effective system for modifying target sites within the genome of a plant, plant cell or seed. Compositions and methods are also provided for producing a plant cell, callus tissue or plant having a modified nucleotide sequence in its genome, without the use of a selectable marker. | 2018-09-27 |
20180273961 | A CRISPR/Cas9 SYSTEM FOR HIGH EFFICIENT SITE-DIRECTED ALTERING OF PLANT GENOMES - Cassettes comprising a YAO promoter operably linked to at least one nucleotide sequence encoding a nuclease, vectors comprising the same are provided. A system for altering a plant genome comprising a nucleotide sequence encoding a nuclease operably linked to a YAO promoter and a method to alter the target nucleic acid molecule by using the system are provided. Plants, progeny and seeds thereof having such altered target nucleic acid molecules are also provided. | 2018-09-27 |
20180273962 | RECOMBINANT MAIZE B CHROMOSOME SEQUENCE AND USES THEREOF - The present disclosure provides maize B chromosome genomic loci and methods of using for agronomic practices. | 2018-09-27 |
20180273963 | SIMULTANEOUS GENE EDITING AND HAPLOID INDUCTION - The presently disclosed subject matter relates to using a haploid inducing line (whether existing or created) and transforming the haploid line so that it encodes cellular machinery capable of editing genes. The transformed haploid inducing line is used as a parent in a cross between two plants. During pollination, the parental gametes fuse to form an embryo; and the gene editing machinery is also delivered to the embryo at this time. During embryonic development, one set of parental chromosomes are lost, and the gene editing machinery operates on the remaining set of chromosomes. Thus, at least one haploid progeny with edited genes is produced from the cross. | 2018-09-27 |
20180273964 | BREEDING METHODS TO DEVELOP IMPROVED XENIA POLLINATORS - This invention describes a new, high-efficiency method of selecting and advancing pollen donator strains in a breeding or product advancement program, wherein the pollen donator strains are specifically selected to maximize product attributes. Embodiments of this invention relate to the use of a mix of pollen from multiple potential pollen donator strains to cross-pollinate a female corn plant, allowing for single-plant performance comparisons. The comparisons of products from the single plant or less experimental unit allow for the selection of those pollen donator strains that maximize desirable results. | 2018-09-27 |
20180273965 | HSF-LIKE TRANSCRIPTION FACTOR, TBF1, IS A MAJOR MOLECULAR SWITCH FOR GROWTH-TO-DEFENSE TRANSITION IN PLANTS - The present invention relates to new methods to study and control the expression of plant genes, particularly nucleotide sequences located downstream from regions comprising binding sites for transcription factors, such as the cis-element translocon 1 (TL1) comprising GAAGAAGAA and similar sequences. The invention relates to isolated nucleotide sequences comprising a regulatory region comprising a promoter operably-linked to one or more upstream open reading frames (uORFs) and one or more downstream open reading frames (dORFs) encoding one or more functional polypeptides, including transcription factors such as TBF1, reporter polypeptides, and polypeptides conferring resistance to drugs, resistance of plants viral, bacterial, or fungal pathogens, and polypeptides involved in the growth of plants. Related aspects include the use of a region which encodes one or more polypeptides designated uORF1 and uORF2 from | 2018-09-27 |
20180273966 | SYNTAXIN 7 NUCLEIC ACID MOLECULES TO CONTROL COLEOPTERAN AND HEMIPTERAN PESTS - This disclosure concerns nucleic acid molecules and methods of use thereof for control of insect pests through RNA interference-mediated inhibition of target coding and transcribed non-coding sequences in insect pests, including coleopteran and/or hemipteran pests. The disclosure also concerns methods for making transgenic plants that express nucleic acid molecules useful for the control of insect pests, and the plant cells and plants obtained thereby. | 2018-09-27 |
20180273967 | REGULATING ALKALOIDS - MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in | 2018-09-27 |
20180273968 | BRASSICA NAPUS SEED SPECIFIC PROMOTERS IDENTIFIED BY MICROARRAY ANALYSIS - Provided are constructs and methods for expressing a transgene in plant cells and/or plant tissues using gene regulatory elements obtained from | 2018-09-27 |
20180273969 | PLANT PROMOTER FROM COTTON AND USES THEREOF - The present disclosure provides a cotton promoter, designated “p2”, which exhibits promoter activity. Interestingly, the promoter is also influenced by water or salt stress. Deletion analysis reveals upstream elements/motifs in the promoter which influence promoter activity, and sequences that are potentially responsive to salt or water stress. | 2018-09-27 |
20180273970 | PLASMID, TRANSFORMED PLANT CELL AND TRANSGENIC PLANT COMPRISING THE SAME, AND METHODS FOR PREPARING A TRANSGENIC PLANT AND FOR INCREASING YIELD OF A PLANT UNDER ABIOTIC STRESSES - A method for increasing yield of a plant, and particularly a method for increasing yield of a plant under abiotic stresses. The method includes preventing or reducing antagonism of Snf1 protein kinase (SnRK1A) by a protein encoded by SEQ ID No: 2 or SEQ ID No: 4. | 2018-09-27 |
20180273971 | Reduced Gluten Grains and Compositions Thereof - Plants with reduced gluten grains and compositions thereof are disclosed herein. | 2018-09-27 |
20180273972 | METHODS OF INCREASING VIRUS RESISTANCE IN CUCUMBER USING GENOME EDITING AND PLANTS GENERATED THEREBY - A cucumber plant comprising a genome being homozygous for a loss of function mutation in an eIF4E gene is provided. Also provided are methods of producing such plants. | 2018-09-27 |
20180273973 | RHIZOMANIA-RESISTANT GENE - The present invention provides a new nucleic acid molecule which encodes a polypeptide that is able to convey a resistance to a pathogen, in particular to “Beet Necrotic Yellow Vein Virus” in a plant, in particular a plant of the | 2018-09-27 |
20180273974 | INSECTICIDAL PROTEINS AND METHODS FOR THEIR USE - Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity. | 2018-09-27 |
20180273975 | MAIZE EVENT DP-032218-9 AND METHODS FOR DETECTION THEREOF - The disclosure provides DNA compositions that relate to transgenic insect resistant maize plants. Also provided are assays for detecting the presence of the maize DP-032218-9 event based on the DNA sequence of the recombinant construct inserted into the maize genome and the DNA sequences flanking the insertion site. Kits and conditions useful in conducting the assays are provided. | 2018-09-27 |
20180273976 | ACTIVATION OF TASTE RECEPTOR GENES IN MAMMALIAN CELLS USING CRISPR-CAS-9 - Suggested is a method for enhancing the expression of taste related receptor genes encompassing the following steps: (i) providing a culture of mammalian cells, the genome of said cells comprising at least one sweet receptor domain; (ii) designing at least one type of single-guide RNA (sgRNA), the 10 to 30 nt guide sequence of said sgRNA being complementary to stretches within the non-coding and/or putative regulatory region upstream of the translation start codon of at least one sweet receptor gene; (iii) preparing a vector comprising an expression cassette encompassing at least one optionally modified CRISPR-Cas9, preferably CRISPR-dCas9VP64, and at least one optionally modified sg-RNA optionally containing aptamer structures for binding activator proteins; (iv) transfecting said culture of mammalian cells with said vector to target the genome for the presence of a DNA sequence that is complementary to the 10 to 30 nt guide sequence of said sgRNA; and (v) measuring the transcriptional enhancement of the sweet receptor mRNA by quantitative RT-PCR. | 2018-09-27 |
20180273977 | MULTIMERIC MRNA - Aspects of the disclosure relate to multimeric molecules and methods of producing the same. In some embodiments, the multimeric molecules comprise at least two nucleic acid molecules (e.g., mRNA molecules) joined by non-covalent bonds between non-coding regions. | 2018-09-27 |
20180273978 | DELIVERY OF BIOMOLECULES INTO CELLS THROUGH CARBON NANOTUBE ARRAYS - An apparati and methods relating to carbon nanotube arrays and their use in administering agents to a cell. | 2018-09-27 |
20180273979 | PROTECTED DNA TEMPLATES FOR GENE MODIFICATION AND INCREASED HOMOLOGOUS RECOMBINATION IN CELLS AND METHODS OF USE - Compositions and methods are provided for modifying a nucleotide sequence in the genome of a cell. The methods and compositions employ a guide polynucleotide, a protected polynucleotide modification template and a Cas endonuclease to modify a nucleotide sequence and/or to increase the frequency of homologous directed repair. The methods can further be used to decrease the frequency of off-site integration of any modification template. The present disclosure also describes methods for selecting a cell comprising a modified target site in its genome and methods for selecting a cell comprising a polynucleotide of interest inserted into a target site in its genome. | 2018-09-27 |
20180273980 | Chimeric Proteins and Methods of Regulating Gene Expression - The present disclosure provides systems, compositions and methods for regulating expression of a target polynucleotide in a cell. The systems, compositions and methods comprise a chimeric receptor polypeptide comprising a G-protein coupled receptor (GPCR) or a fragment thereof, a chimeric adaptor polypeptide, at least one actuator moiety and a cleavage moiety. | 2018-09-27 |
20180273981 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2018-09-27 |
20180273982 | COMPOSITIONS AND METHODS FOR PRODUCING ISOPRENE - The invention features methods for producing isoprene from cultured cells. The invention also provides compositions that include these cultured cells. | 2018-09-27 |
20180273983 | ALKANE OXIDATION BY MODIFIED HYDROXYLASES - This invention relates to modified hydroxylases. The invention further relates to cells expressing such modified hydroxylases and methods of producing hydroxylated alkanes by contacting a suitable substrate with such cells. | 2018-09-27 |
20180273984 | DETOXIFYING PRE-TREATED LIGNOCELLULOSE-CONTAINING MATERIALS - The invention relates to a process of detoxifying pre-treated lignocellulose-containing material comprising subjecting the pre-treated lignocellulose-containing material to one or more phenolic compound oxidizing enzymes. | 2018-09-27 |
20180273985 | CELL-FREE PRODUCTION OF BUTANOL - Provided herein, in some aspects, are methods and compositions for producing large-scale quantities of butanol, including normal butanol (n-butanol), isobutanol, and 2-butanol using a cell-free system. | 2018-09-27 |
20180273986 | NOVEL ORGANIC ACID PATHWAY - The invention relates to the use of a cytosolic citric acid synthase for the heterologous production of citrate outside the mitochondrion of a micro-organism or algae, wherein the protein is selected from | 2018-09-27 |
20180273987 | METHODS, REAGENTS AND CELLS FOR BIOSYNTHESIZING COMPOUNDS - This document describes biochemical pathways for producing 2,4-pentadienoyl-CoA by forming one or two terminal functional groups, comprised of carboxyl or hydroxyl group, in a C5 backbone substrate such as glutaryl-CoA, glutaryl-[acp] or glutarate methyl ester. 2,4-pentadienoyl-CoA can be enzymatically converted to 1,3-butadiene. | 2018-09-27 |
20180273988 | METHODS & SYSTEMS FOR OBTAINING OIL FROM A STILLAGE COMPOSITION - Provided are methods and systems of obtaining oil from a stillage composition. | 2018-09-27 |
20180273989 | QUALITY CONTROL FOR IMPROVED PRODUCT BIOSYNTHESIS - Systems, methods, and host cells utilizing a PopQC construct for enhancing product biosynthesis by exploitation of non-genetic cell-to-cell variation are disclosed. The PopQC construct includes at least a product-responsive biosensor and a selection gene. | 2018-09-27 |
20180273990 | PRODUCTION OF FATTY ACIDS ESTERS - A microbial cell is used for producing at least one fatty acid ester, wherein the cell is genetically modified to contain (i) at least one first genetic mutation that enables the cell to produce at least one fatty acid and/or acyl coenzyme A (CoA) thereof by increased enzymatic activity in the cell relative to the wild type cell of malonyl-CoA dependent and malonyl-ACP independent fatty acyl-CoA metabolic pathway, wherein the fatty acid contains at least 5 carbon atoms; and (ii) a second genetic mutation that increases the activity of at least one wax ester synthase in the cell relative to the wild type cell and the wax ester synthase has sequence identity of at least 50% to a polypeptide of SEQ ID NO: 1-8 and combinations thereof or to a functional fragment of any of the polypeptides for catalyzing the conversion of fatty acid and/or acyl coenzyme A thereof to the fatty acid ester. | 2018-09-27 |
20180273991 | METHOD FOR PRODUCING MERCAPTANS BY HYDROGEN-ASSISTED DISULFIDE ENZYME HYDROGENOLYSIS - Provided is an enzymatic process for the preparation of a mercaptan of formula R—SH from disulfides utilizing hydrogen. | 2018-09-27 |
20180273992 | MICROORGANISMS FOR PRODUCING DIAMINE AND PROCESS FOR PRODUCING DIAMINE USING THEM - The present invention relates to a microorganism for producing diamine, in which activity of a protein having an amino acid sequence of SEQ ID NO: 6 or an amino acid sequence having 42% or higher sequence homology with SEQ ID NO: 6 is introduced or enhanced, and a method of producing diamine using the same. | 2018-09-27 |
20180273993 | METHOD FOR PRODUCING HYDROXY-L-PIPECOLIC ACID - A novel method of producing high-purity hydroxy-L-pipecolic acids in an efficient and inexpensive manner while suppressing the production of hydroxy-L-proline is provided. The method includes allowing an L-pipecolic acid hydroxylase, a microorganism or cell having the ability to produce the enzyme, a processed product of the microorganism or cell, and/or a culture liquid comprising the enzyme and obtained by culturing the microorganism or cell, to act on L-pipecolic acid as a substrate in the presence of 2-oxoglutaric acid and ferrous ion, wherein the L-pipecolic acid hydroxylase has the properties:
| 2018-09-27 |
20180273994 | METHOD FOR PREPARING PSICOSE - A method for obtaining psicose from a mixture of fructose and psicose includes adding a mannitol dehydrogenase to a mixture of fructose and psicose to convert fructose into mannitol, and separating the mannitol from the psicose. Accordingly, the psicose can be more easily isolated from the mannitol with high efficiency by using the method. | 2018-09-27 |
20180273995 | GLUCOAMYLASE BLENDS - Methods are provided for saccharifying a starch substrate, comprising contacting the starch substrate with a glucoamylase consisting or comprising of the amino acid sequence of | 2018-09-27 |
20180273996 | FERMENTATION PROCESS FOR PRODUCING MONOSACCHARIDES IN FREE FORM FROM NUCLEOTIDE-ACTIVATED SUGARS - The present invention relates to a process for producing a monosaccharide, e.g. L-fucose, in free form using a microbial fermentation process. The used microorganism exhibits hydrolase activity on nucleotide-activated sugars and releases the monosaccharide in an unmodified free form. The free monosaccharide is retrieved from the supernatant of the cultivated microorganism. | 2018-09-27 |
20180273997 | CELLULOLYTIC ENZYME COMPOSITION - Compositions comprising an inactive cellulosome-producing microorganism and detectable extra-cellular osidase are provided. Compositions comprising an inactive | 2018-09-27 |
20180273998 | PROCESSES FOR PRODUCING FERMENTATION PRODUCTS - The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and a thermostable endoglucanase is present and/or added during liquefaction. The invention also relates to compositions suitable for use in processes of the invention. | 2018-09-27 |
20180273999 | ENZYMATIC HYDROLYSIS OF DISACCHARIDES AND OLIGOSACCHARIDES USING ALPHA-GLUCOSIDASE ENZYMES - A method is disclosed for hydrolyzing an alpha-1,5 glucosyl-fructose linkage in a saccharide (disaccharide or oligosaccharide) such as leucrose. This method comprises contacting the saccharide with an alpha-glucosidase enzyme such as transglucosidase or glucoamylase under suitable conditions, during which contacting step the enzyme hydrolyzes at least one alpha-1,5 glucosyl-fructose linkage of the saccharide. This method is useful for reducing the amount of leucrose in a filtrate isolated from a glucan synthesis reaction, for example. | 2018-09-27 |
20180274000 | Self-Sufficient Process For The Production Of Biomass Hydrolysate With Reduced Salt Content - The present invention is directed to a self-sufficient process for the production of biomass hydrolysate with reduced salt content as well as the de-salted hydrolysate produced after the inventive process and the use of the de-salted hydrolysate as a fermentation medium. | 2018-09-27 |
20180274001 | NUCLEIC ACID SYNTHESIS USING DNA POLYMERASE THETA - Provided herein are methods for template-independent synthesis of oligonucleotides using a DNA polymerase. Also provided are methods for template-directed synthesis of oligonucleotides and for sequencing of nucleic acids using DNA polymerase theta and 3′-aminoalkoxy nucleotides. | 2018-09-27 |
20180274002 | NOVEL METHOD FOR THE CULTURE OF UNICELLULAR RED ALGAE - The invention relates to the field of algaculture, particularly the culture of unicellular red algae (URA). In particular, the invention relates to a method for the culture of unicellular red algae, characterised in that the specific culture conditions, in terms of lighting and nutrients, allow the production of a protein-rich biomass that can contain an increased amount of URA and produce, in addition to pigments, particularly phycocyanin and carotenoids: β-carotene and zeaxanthin. The invention also relates to the biomass that can be produced using the method of the invention, to the uses of the biomass and to products that can contain said biomass. | 2018-09-27 |
20180274003 | BUTELASE-MEDIATED PEPTIDE LIGATION - The present invention relates to a method of forming a peptide of Formula (I) (P | 2018-09-27 |
20180274004 | RECOMBINANT PHAGE AND METHODS OF DETECTING LISTERIA - Composition and methods for the detection of one or more target microbe(s) are provided. Compositions of the disclosure include at least one recombinant phage capable of infecting a target microbe, said phage comprising at least a capsid protein sequence, a ribosome binding site, and a codon-optimized marker. Compositions of the disclosure may further include an aqueous solution that enhances the ability to detect marker expression upon phage infection of the target microbe. In some embodiments the target microbe is | 2018-09-27 |
20180274005 | CORD BLOOD THERAPY TO TREAT CHRONIC DISEASE CAUSED BY L-FORM BACTERIA - The present disclosure describes screening cord blood for antibacterial activity against L-form bacteria, methods of treating a patient having an L-form bacterial infection using a cord blood agent, and methods of killing or inhibiting L-form bacteria in an in vitro or ex vivo setting. L-form bacteria underlying an L-form bacterial infection are isolated from a subject having an infection and are altered to a culturable morphology. A set of cord blood agents are screened against the isolated L-form bacteria to identify one or more cord blood agents usable as effective treatment agents. | 2018-09-27 |
20180274006 | METHOD FOR DETECTING MOLECULAR INTERACTIONS USING AN IMMOBILIZED NUCLEIC ACID - The present invention relates to a method for detecting and/or characterizing molecular interactions between two molecules, in particular two proteins and most particular between an antigen and an antibody by using an immobilized single-stranded nucleic acid molecule to which single-stranded nucleic acid molecules having a ligand attached thereto are hybridized. | 2018-09-27 |
20180274007 | METHODS OF GENOME SEQENCING AND EPIGENETIC ANALYSIS - Novel methods of ChIP-seq are disclosed herein. These methods of ChIP-seq employ carrier DNA to prevent loss of DNA samples. The greater DNA yields achieved by this invention permit ChIP-seq of a small number of cells, permitting epigenetic analysis of primary cells of limited quantity. | 2018-09-27 |
20180274008 | METHODS AND SYSTEMS FOR USING ENCAPSULATED MICROBUBBLES TO PROCESS BIOLOGICAL SAMPLES - Methods and systems for using encapsulated microbubbles to process biological samples are disclosed. According to one aspect, a method for using encapsulated microbubbles to process a biological sample includes creating a mixture comprising encapsulated microbubbles mixed with a biological sample and adding activation energy to the mixture to cause at least some of the microbubbles to oscillate or burst and thereby process the sample, including effecting cell lysis, shearing DNA, and/or performing tissue dispersion. | 2018-09-27 |
20180274009 | METHODS AND COMPOSITIONS FOR RNA MAPPING - Novel methods for identification and analysis of mRNA are provided herein. The methods may involve digestion and fingerprinting analysis. | 2018-09-27 |
20180274010 | BIOMARKER COMPOSITION, DIAGNOSTIC KIT, AND METHOD FOR PROVIDING INFORMATION - Provided are a biomarker composition that includes a nucleotide with a nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 2 as an active ingredient, a diagnostic kit that includes the biomarker composition, and a method of providing information for diagnosis of atopic dermatitis (AD) that includes detecting a subspecies of | 2018-09-27 |
20180274011 | METHODS AND KITS TO DETECT AND GENOTYPE CRYPTOCOCCUS SPECIES - Embodiments of the invention provide a method of genotyping a | 2018-09-27 |
20180274012 | COMPOSITIONS AND METHODS FOR DETECTING OR QUANTIFYING PARAINFLUENZA VIRUS - Compositions, methods, kits, and uses are provided for detecting or quantifying an Human Parainfluenza virus 1 (HPIV-1), HPIV-2, HPIV-3, and/or HPIV-4 nucleic acid, e.g., using nucleic acid amplification and hybridization assays. In some embodiments, the compositions, methods, kits, and uses target the HN gene of HPIV-1, HPIV-2, and/or HPIV-3 and/or the NP gene of HPIV-4. | 2018-09-27 |
20180274013 | METAL-ORGANIC FRAMEWORK NANOSHEET - A method of preparing a metal-organic framework nanosheet is provided. The method includes providing a mixture comprising a metal precursor, a ligand, and a surfactant by at least substantially dissolving the metal precursor, the ligand, and the surfactant in a suitable solvent, and heating the mixture to obtain the metal-organic framework nanosheet. A metal-organic framework nanosheet, methods of preparing a metal-organic framework membrane and a composite material, and applications of the nanosheet and/or membrane in sensing and separation are also provided. | 2018-09-27 |