38th week of 2015 patent applcation highlights part 24 |
Patent application number | Title | Published |
20150259638 | BIOREACTOR, REACTOR BAG FOR SAME AND STIRRER FOR CIRCULATING CONTENTS OF SAID BAG - A bioreactor has a reactor bag ( | 2015-09-17 |
20150259639 | GAS-FED FERMENTATION SYSTEMS - A fermenter can have at least one hollow fluid conduit disposed at least partially within a vessel. An external circumference of the hollow fluid conduit and an interior circumference of the vessel can define a downward flow path through which a multi-phase mixture including a liquid media and compressed gas substrate bubbles flows. An interior circumference of the hollow fluid conduit can defined an upward flow path which is in fluid communication with the downward flow path. The multi-phase liquid can flow through the upward flow path and exit the fermenter. Cooling may be provided in the hollow fluid conduit or the vessel. One or more backpressor generators can be used to maintain a backpressure on the fermenter. One or more fluid movers can be used to variously create an induced and/or forced flow in the downward and upward flow paths. | 2015-09-17 |
20150259640 | CELL CULTURE VESSEL, CELL CULTURING APPARATUS, AND CELL CULTURING METHOD - In order to determine the culturing state of cells in a culture vessel in a simple manner at an appropriate timing without removing the culture vessel from the culturing apparatus, a cell culturing apparatus includes a culturing chamber that accommodates a culture vessel, which can accommodate cells in the interior thereof, and that maintains a predetermined temperature and humidity; a touchscreen sensor that is disposed inside the culturing chamber on a mounting surface, on which the culture vessel is placed, and that is brought into tight contact with an external surface of a bottom of the culture vessel; and an output portion that exports an output from the touchscreen sensor to the exterior of the culturing chamber. | 2015-09-17 |
20150259641 | GELATINOUS SUBSTANCE BASED GROWTH MEDIUM FILTRATION DEVICE AND METHOD - Growth medium filtration devices are provided which are particularly well adapted to sterilize gelatinous substance based growth mediums including agar, agarose and other polysaccharide derived growth mediums for use in agar plates and the like for culturing microorganisms. The devices may include one or more filtration units and at least one heat source for filtering the growth medium in a heated filtration process. Methods of filtering a growth medium, such as an agar growth medium, and methods of preparing a growth medium having low gelling point and lower molecular weight characteristics are also provided. | 2015-09-17 |
20150259642 | METHODS AND COMPOSITIONS FOR DEGRADING OIL SLUDGE - Methods and systems for degrading oil sludge are disclosed. In one embodiment, a method of degrading an oil sludge in a pipeline may involve introducing a microbial mixture comprising a | 2015-09-17 |
20150259643 | Cell Preparation Method - A method of thawing cryopreserved human hepatocytes and cryopreserving the hepatocytes a second time without losing viability is described. It allows the preparation of cryopreserved human hepatocytes pooled from multiple donors by thawing the hepatocytes from the individual donors, combining the cells to form pooled hepatocytes, and recryopreserving the pooled hepatocytes. The method involves the thawing of the hepatocytes from the individual donors, maintaining the thawed hepatocytes at a low temperature that is above freezing temperature, and refreezing the thawed cells without further manipulation. The method allows the cryopreserving the human hepatocytes a second time after thawing, with viability similar to that after one single cryopreservation. This high efficiency method can be used for the preparation of highly viable pooled human hepatocytes for experimentation to minimize individual variations. | 2015-09-17 |
20150259644 | HORMONE RESPONSIVE TISSUE CULTURE SYSTEM AND USES THEREOF - The invention provides tissue culture system for primary cells (e.g. normal mammalian primary epithelial progenitors). This system includes: a) a serum-free, chemically defined cell culture media; and, b) methods for isolation and in vitro long-term propagation of primary cells (e.g. primary epithelial cells). Primary cells so isolated and cultured can be kept undifferentiated and proliferate for many weeks (>15 weeks) or population doubling (>35 PD) without senescence, or any detectable genetic alterations. Upon changing media/culture conditions, these cells can be induced to differentiate. | 2015-09-17 |
20150259645 | METHOD FOR INDUCING IL-2-FREE PROLIFERATION OF GAMMA DELTA T CELLS - The present invention concerns a method of inducing IL-2-free proliferation of γδ T cells using a combination of a γδ T cell activator and IL-33 for use in therapy of infection, cancer, autoimmunity as well as other diseases. | 2015-09-17 |
20150259646 | Methods for Isolating and Proliferating Autologous Cancer AntiGen-Specific CD8+ T Cells - Provided is a method for isolating and proliferating autologous cancer antigen-specific CD8 | 2015-09-17 |
20150259647 | HUMAN ADIPOSE TISSUE WHITE AND 'BROWN-ON-WHITE' PROGENITORS FOR RECONSTRUCTIVE AND METABOLIC THERAPIES - Methods for preparation of prospectively identified human adipose stem cells enriched populations thereof, e.g., for therapy. | 2015-09-17 |
20150259648 | METHODS OF DIFFERENTIATING STEM CELLS INTO CHONDROCYTES - Disclosed herein are methods of producing chondrocytes from pluripotent stem cells. The invention further provides methods of regenerating cartilaginous tissue. | 2015-09-17 |
20150259649 | CELLULAR COMPOSITIONS USED TO RESTORE STEM CELL OR PROGENITOR CELL FUNCTION AND METHODS RELATED THERETO - This disclosure relates to compounds, compositions and methods of epigenetically transforming cells. In certain embodiments, the disclosure relates to methods of generating epigenetically altered cells comprising mixing isolated cells with compositions disclosed herein under conditions such that epigenetically altered cells are formed. In certain embodiments, methods of treating or preventing vascular or diabetic diseases or conditions are contemplated. In certain embodiments, epigenetically reprogramming adult bone marrow-derived stem or progenitor cells including mesenchymal stem cells (MSCs) or endothelial progenitor cells (EPCs) for autologous treatments are contemplated. | 2015-09-17 |
20150259650 | RELIABILITY OF ASSAYS USING A MULTI-DIVOT PLATFORM AND MULTI-SOURCE, MULTI-CELL TYPE CLUSTERS - Described herein are 3-dimensional clusters of reaggregated cells comprising cells reaggregated from at least two different cell sources, such as different cell types, different donors, and combinations thereof. Methods of making, using, and cryopreserving these 3-dimensional clusters of reaggregated cells are also described herein. | 2015-09-17 |
20150259651 | POLYPEPTIDES HAVING PEROXYGENASE ACTIVITY - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. A polynucleotide encoding a peroxygenase was isolated from | 2015-09-17 |
20150259652 | CHIMERIC GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF - A novel luciferase that is distinct from conventional luciferase has been desired. | 2015-09-17 |
20150259653 | TRITERPENE OXIDASE DERIVED FROM PLANT BELONGING TO GENUS GLYCHYRRHIZA, GENE ENCODING THE TRITERPENE OXIDASE, AND USE OF THE PROTEIN OR THE GENE - The present invention provides a protein having an activity of oxidizing a dammarane-type triterpene, a gene encoding the same, and use of the protein and the gene. The present invention specifically relates to a protein obtainable from a plant belonging to the genus | 2015-09-17 |
20150259654 | COMPOSITIONS AND METHODS FOR MODIFYING CELL SURFACE GLYCANS - Methods and compositions for modifying glycans (e.g., glycans expressed on the surface of live cells or cell particles) are provided herein. | 2015-09-17 |
20150259655 | CHROMOSOMAL MODIFICATION INVOLVING THE INDUCTION OF DOUBLE-STRANDED DNA CLEAVAGE AND HOMOLOGOUS RECOMBINATION AT THE CLEAVAGE SITE - Methods of modifying, repairing, attenuating and inactivating a gene or other chromosomal DNA in a cell are disclosed. Also disclosed are methods of treating or prophylaxis of genetic disease in an individual in need thereof. Further disclosed are chimeric restriction endonucleases. | 2015-09-17 |
20150259656 | MODIFIED FUNGAL CELL - The present invention relates to a genetically modified fungal cell of the genus | 2015-09-17 |
20150259657 | STABLE FUNGAL CEL6 ENZYME VARIANTS - The disclosure provides variant Cel6a enzymes having increased thermostability, methods of making and using such polypeptides. | 2015-09-17 |
20150259658 | ENGINEERED YEAST FOR CELLULOSIC ETHANOL PRODUCTION - The disclosure provides designer cellulosomes for efficient hydrolysis of cellulosic material and more particularly for the generating of ethanol. | 2015-09-17 |
20150259659 | METHOD FOR OBTAINING NATURAL VARIANT OF ENZYME AND SUPER THERMOSTABLE CELLOBIOHYDROLASE - A method for selectively obtaining a natural variant of an enzyme having activity includes (1) a step of detecting an ORF sequence of a protein having enzyme activity from a genome database including base sequences of metagenomic DNA of environmental microbiota; (2) a step of obtaining at least one PCR clone including the ORF sequence having a full length, a partial sequence of the ORF sequence, or a base sequence encoding an amino acid sequence which is formed by deletion, substitution, or addition of at least one amino acid residue in an amino acid sequence encoded by the ORF sequence, by performing PCR cloning on at least one metagenomic DNA of the environmental microbiota by using a primer designed based on the ORF sequence; (3) a step of determining a base sequence and an amino acid sequence which is encoded by the base sequence for each PCR clone obtained in the step (2); and (4) a step of selecting a natural variant of an enzyme having activity by measuring enzyme activity of proteins encoded by each PCR clone obtained in the step (2). | 2015-09-17 |
20150259660 | THERMOSTABLE CELLOBIOHYDROLASE AND AMINO ACID SUBSTITUTED VARIANT THEREOF - A thermostable cellobiohydrolase including a cellobiohydrolase catalytic domain, the cellobiohydrolase catalytic domain including:
| 2015-09-17 |
20150259661 | Polypeptides Having Endoglucanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having endoglucanase activity, catalytic domains, cellulose binding domains and polynucleotides encoding the polypeptides, catalytic domains or cellulose binding domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or cellulose binding domains. | 2015-09-17 |
20150259662 | POLYPEPTIDES HAVING PROTEASE ACTIVITY - The present invention relates to isolated polypeptides having protease activity and isolated nucleic acid sequences encoding the proteases. The invention also relates to nucleic acid constructs, vectors, and host cells, including plant and animal cells, comprising the nucleic acid sequences, as well as methods for producing and using the proteases, in particular the use of the proteases in animal feed. | 2015-09-17 |
20150259663 | Metalloproteases from Alicyclobacillus - The present invention relates to new metalloproteases derived from | 2015-09-17 |
20150259664 | PROTEOLYTIC INACTIVATION OF SELECT PROTEINS IN BACTERIAL EXTRACTS FOR IMPROVED EXPRESSION - The present disclosure provides modified proteins that are capable of being cleaved by the protease OmpT1. The proteins can be modified in an exposed surface motif to incorporate OmpT1 cleavage sites. Also provided are nucleic acids encoding the modified proteins, bacterial cells that express the modified proteins, and cell free synthesis systems containing modified RF1. The disclosure further provides methods for reducing the deleterious activity of a modified protein in a cell free synthesis system by contacting the modified protein with OmpT1. Also provided are methods for reducing RF1 competition at an amber codon in the cell free synthesis system, and methods for expressing a protein in the cell free synthesis system. The modified proteins of the invention can be used to increase the yield of proteins having non-natural amino acids incorporated at an amber codon. | 2015-09-17 |
20150259665 | FACTOR VII CONJUGATES - The present invention relates to the conjugation of Factor VII polypeptides with heparosan polymers. The resultant conjugates may be used to deliver Factor VII, for example in the treatment or prevention of bleeding disorders. | 2015-09-17 |
20150259666 | CHIMERIC TISSUE PLASMINOGEN ACTIVATOR (T-PA) RESIATANT TO PLASMINOGEN ACTIVATOR INHIBITOR-1 AND IMPROVED BIOCHEMICAL PROPERTIES - The present invention discloses the thrombolytic therapy by t-PA or CT-b for the treatment of the acute myocardial infarction. A chimeric truncated form of t-PA or CT-b is designed and expressed in | 2015-09-17 |
20150259667 | PECTIN DEGRADING ENZYMES FROM MACROPHOMINA PHASEOLINA AND USES THEREOF - The present invention discloses isolated polynucleotide encoding enzymes, derived from the fungus | 2015-09-17 |
20150259668 | BIOACTIVE COMPOSITIONS FOR HIGH AVIDITY CELL CAPTURE - A composition useful for cell capture, the composition comprising a solid substrate on which is affixed a patterned polymer, and a cell-targeting agent attached to said patterned polymer, wherein said cell-targeting agent is exposed. Also described is a method for the preparation of the cell capturing composition, as well as flow through devices in which the cell capturing composition is incorporated. Further described is a method of capturing cells by contacting the cell-capturing composition with a liquid or gaseous sample containing cells. The method for capturing cells may also be a method for testing for the presence of one or more classes or species of cells or cellular organisms in a liquid or gaseous sample. | 2015-09-17 |
20150259669 | MICROBIAL ELECTROSYNTHETIC CELLS - Methods are provided for microbial electrosynthesis of H | 2015-09-17 |
20150259670 | SYSTEM FOR OPTICAL STIMULATION OF TARGET CELLS - Various systems and methods are implemented for controlling stimulus of a cell. One such method is implemented for optical stimulation of a cell expressing a NpHR ion pump. The method includes the step of providing a sequence of stimuli to the cell. Each stimulus increases the probability of depolarization events occurring in the cell. Light is provided to the cell to activate the expressed NpHR ion pump, thereby decreasing the probability of depolarization events occurring in the cell. | 2015-09-17 |
20150259671 | DEVICES AND SYSTEMS FOR ISOLATING BIOMOLECULES AND ASSOCIATED METHODS THEREOF - A device, a system, a cartridge and a method for isolating biomolecules from biological materials are provided. The device comprises a substrate; a reagent storage location; and a self-rupturing component comprising a fluid and a pressure source embedded therein, wherein the substrate, the reagent storage location and the self-rupturing component are operationally coupled to each other. A system is provided, wherein the system comprises an extraction matrix, an enclosed matrix housing comprising a biological sample inlet, one or more biomolecule extraction reagents to extract biomolecules and at least one pressure source embedded therein, a fluidic extraction circuit; and a controller for activating the embedded pressure source. A method of isolating nucleic acids from biological materials is also provided. | 2015-09-17 |
20150259672 | Nucleic Acid Purification - A self-contained apparatus for isolating nucleic acid, cell lysates and cell suspensions from unprocessed samples apparatus, to be used with an instrument, includes at least one input, and: (i) a macrofluidic component, including a chamber for receiving an unprocessed sample from a collection device and at least one filled liquid purification reagent storage reservoir; and (ii) a microfluidic component in communication with the macrofluidic component through at least one microfluidic element, the microfluidic component further comprising at least one nucleic acid purification matrix; and (iii) at least one interface port to a drive mechanism on the instrument for driving said liquid purification reagent, through the microfluidic element and the nucleic acid purification matrix, wherein the only inputs to the apparatus are through the chamber and the interface port to the drive mechanism. | 2015-09-17 |
20150259673 | Library Compositions and Methods for Acyclic Identification of Aptamers - The present invention provides improved aptamer libraries useful for discovery of aptamers that have high binding affinity for a single or a plurality of targets. The libraries contain higher copies of each member candidate such that they are more likely to be available to the application of acyclic identification methods that obviate the most time-consuming and costly step in traditional SELEX method, the multiple cycles of evolutionary selection. | 2015-09-17 |
20150259674 | COMPOSITIONS AND METHODS FOR LONG INSERT, PAIRED END LIBRARIES OF NUCLEIC ACIDS IN EMULSION DROPLETS - The invention provides for methods for uniquely labeling populations of nucleic acids of interest in emulsion droplets using random combinations of oligonucleotides. The labeling methodology of the invention may be used, inter alia, to generate mate pair genomic fragments without the need for circularization. Because the method is independent of circularization, mate pairs can be generated from any length genomic fragment. | 2015-09-17 |
20150259675 | SHORT INTERFERING RIBONUCLEIC ACID (siRNA) FOR ORAL ADMINISTRATION - Short interfering ribonucleic acid (siRNA) for oral administration, said siRNA comprising two separate RNA strands that are complementary to each other over at least 15 nucleotides, wherein each strand is 49 nucleotides or less, and wherein at least one of which strands contains at least one chemical modification. | 2015-09-17 |
20150259676 | DOUBLE-STRANDED NUCLEIC ACID COMPOUNDS - The present invention provides double-stranded RNA (dsRNA) compounds comprising a sense and antisense strand, wherein at least one strand is conjugated to a moiety comprising a phenyl hydrocarbyl group, pharmaceutical compositions comprising same and uses thereof. | 2015-09-17 |
20150259677 | siRNA and their use in methods and compositions for the treatment and / or prevention of eye conditions - The present invention relates to methods, compositions and dosages that decrease IOP of the eye, comprising a 19 nucleotide double-stranded RNA molecule. | 2015-09-17 |
20150259678 | HYDROXYLATED POLYAMINE DERIVATIVES AS TRANSFECTION REAGENTS - The invention provides hydroxylated polyamine derivatives, their use for the transfection of polyanions into cells, and a method of transfecting cells with a polyanion, comprising mixing said polyanion with said hydroxylated polyamine derivative in a buffer and treating said cells with the mixture obtained in the previous step. | 2015-09-17 |
20150259679 | COMPOSITIONS FOR MODULATING C9ORF72 EXPRESSION - Disclosed herein are compositions and methods for reducing expression of C90RF72 mRNA and protein in an animal with C90RF72 specific inhibitors. Such methods are useful to treat, prevent, or ameliorate neurodegenerative diseases in an individual in need thereof. Such C90RF72 specific inhibitors include antisense compounds. Examples of neurodegenerative diseases that can be treated, prevented, and ameliorated with the administration C90RF72 specific inhibitors include amyotrophic lateral sclerosis (ALS), frontotemporal dementia (FTD), corticalbasal degeneration syndrome (CBD), atypical Parkinsonian syndrome, and olivopontocerellar degeneration (OPCD). | 2015-09-17 |
20150259680 | NUCLEIC ACID FUNCTIONALIZED NONOPARTICLES FOR THERAPEUTIC APPLICATIONS - Materials and methods for regulating gene expression using nanoparticles functionalized with antisense oligonucleotides are provided. | 2015-09-17 |
20150259681 | OLIGOMERIC COMPOUNDS COMPRISING TRICYCLIC NUCELOSIDES AND METHODS FOR THEIR USE - The present disclosure provides tricyclic nucleosides, oligomeric compounds comprising at least one of the tricyclic nucleosides and methods of using the oligomeric compounds. The methods provided herein include contacting a cell or administering to an animal at least one of the oligomeric compounds. In certain embodiments, the oligomeric compounds hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. | 2015-09-17 |
20150259682 | GENE SILENCING METHODS - Provided are methods and means to obtain improved gene silencing of target nucleic acids whereby at least two inhibitory RNA molecules are provided which are targeted to the same nucleic acid, but which are processed into short interfering RNA molecules through different processing pathways. Also provided are methods and means to obtain improved gene silencing of target nucleic acids whereby at least two inhibitory RNA molecules are provided which are targeted to different nucleic acids, but which are processed into short interfering RNA molecules through different processing pathways. | 2015-09-17 |
20150259683 | AGENT FOR TREATING RENAL FIBROSIS - The present invention relates to a substance delivery carrier for extracellular matrix-producing cells in the kidney, the carrier including a retinoid as a targeting agent, an agent for treating renal fibrosis utilizing the carrier, a process for producing them, a production kit, a method for treating renal fibrosis using the agent for treating renal fibrosis, etc. | 2015-09-17 |
20150259684 | Orthogonal Cas9 Proteins for RNA-Guided Gene Regulation and Editing - Methods of modulating expression of a target nucleic acid in a cell are provided including use of multiple orthogonal Cas9 proteins to simultaneously and independently regulate corresponding genes or simultaneously and independently edit corresponding genes. | 2015-09-17 |
20150259685 | TREATMENT OF UNCOUPLING PROTEIN 2 (UCP2) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO UCP2 - The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Uncoupling Protein 2 (UCP2), in particular, by targeting natural antisense polynucleotides of Uncoupling Protein 2 (UCP2). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of UCP2. | 2015-09-17 |
20150259686 | NICKED OR GAPPED NUCLEIC ACID MOLECULES AND USES THEREOF - The present disclosure provides meroduplex (nicked or gapped) ribonucleic acid molecules (mdRNA) that decreases or silences target gene expression. An mdRNA of this disclosure comprises at least three strands that combine to form at least two non-overlapping double-stranded regions separated by a nick or gap wherein one strand is complementary to a target gene RNA. In addition, the meroduplex may have one or more modifications or substitutions, such as nucleotide base, sugar, terminal cap structure, internucleotide linkage, or any combination of such modifications. Also provided are methods of decreasing expression of a target gene in a cell or in a subject to treat a disease related to altered expression of a target gene. | 2015-09-17 |
20150259687 | Single Stranded DNA Aptamers Binding NF-kB/RelA - DNA aptamers are high affinity ligands selected by genetic enrichment techniques to bind to specific protein targets. Because these represent chemically stable and reproducible molecules, they have application as affinity reagents and/or therapeutic drugs to affect the target protein's actions. NF-kB is an important mediator of the innate immune response and mediator of tissue inflammation. Although RNA and double stranded DNA aptamers have been identified to bind to the NF-kB family of proteins, the present invention represents the first identification of single stranded DNA aptamers that recognize NFkB RelA. The aptamers disclosed herein bind to several distinct regions of RelA and may be useful to antagonize the DNA binding of RelA as an inhibitor of cellular inflammation, visualize the location or amount of RelA in tissues from pathological conditions, or to quantitatively measure the activated state of RelA by affinity binding. | 2015-09-17 |
20150259688 | NUCLEIC ACID LIGANDS TO LL37 - The present invention is directed to nucleic acid ligands to LL37, methods for producing said nucleic acid ligands, and methods for utilizing said nucleic acid ligands. In one exemplary embodiment, for example, this invention relates to nucleic acid ligands exhibiting high specific binding affinity to LL37 peptides, precursors and/or portions thereof. Further, the nucleic acid ligands may bind competitively with native ligands of LL37 and may also inhibit and/or interfere with LL37 function, such as by binding to LL37. | 2015-09-17 |
20150259689 | MODULATION OF HUMAN CYTOMEGALOVIRUS REPLICATION BY MICRO-RNA 132 (miR132), MICRO-RNA 145 (miR145) AND MICRO-RNA 212 (miR212) - The present invention related to miR145, miR132, miR212, and the genes or gene products regulated by these miRNAs. miR145 is downregulated in cells infected with HCMV. This downregulation modulates expression of miR145 target genes, including IRS-1. Transfection of cells with a miR145 agent, such as a miR145 mimetic, reduces HCMV replication and protein expression. miR132 and miR212 are upregulated in cells infected with HCMV. This upregulation modulates expression of miR132 and miR212 target genes, including MeCP2 and RICS. Transfection of cells with a miR132 and/or a miR212 antagonist reduces HCMV replication and protein expression. Accordingly, the invention provides methods of attenuating HCMV replication by modulating, for example, miR145, miR132, and/or miR212, and targets thereof. Also provided are methods of detecting an HCMV infection, and compositions and kits useful for attenuating HCMV replication. | 2015-09-17 |
20150259690 | AMPHIREGULIN-SPECIFIC DOUBLE-HELICAL OLIGO-RNA, DOUBLE-HELICAL OLIGO-RNA STRUCTURE COMPRISING DOUBLE-HELICAL OLIGO-RNA, AND COMPOSITION FOR PREVENTING OR TREATING RESPIRATORY DISEASES CONTAINING SAME - An amphiregulin-specific double-stranded oligo siRNA structure comprises hydrophilic and hydrophobic compounds bound to the amphiregulin-specific double-stranded oligo siRNA by a simple covalent bond or a linker-mediated covalent bond so as to increase the in vivo stability and intracellular delivery efficiency of the double-stranded oligo siRNA, and to nanoparticles composed of the oligo siRNA structures. The amphiregulin-specific double-stranded oligo siRNA structure can increase the stability of the amphiregulin-specific double-stranded oligo siRNA without impairing the function of the amphiregulin-specific double-stranded oligo siRNA, and at the same time, can efficiently increase the membrane permeability of the double-stranded oligo siRNA. Thus, when the amphiregulin-specific double-stranded oligo siRNA structure is used, the amphiregulin-specific double-stranded oligo siRNA can be efficiently delivered into cells even when it is administered at a relatively low concentration, so that it can be effectively used for the prevention or treatment of respiratory diseases. | 2015-09-17 |
20150259691 | UGT8 MINI-PROMOTERS - Isolated polynucleotides comprising a UGT8 mini-promoters are provided. The mini-promoter may be operably linked to an expressible sequence, e.g. reporter genes, genes encoding a polypeptide of interest, regulatory RNA sequences such as miRNA, siRNA, anti-sense RNA, etc., and the like. In some embodiments a cell comprising a stable integrant of an expression vector is provided, which may be integrated in the genome of the cell. The promoter may also be provided in a vector, for example in combination with an expressible sequence. The polynucleotides find use in a method of expressing a sequence of interest, e.g. for identifying or labeling cells, monitoring or tracking the expression of cells, gene therapy, etc. | 2015-09-17 |
20150259692 | COMPOSITION AND METHODS FOR THE PREVENTION AND TREATMENT OF DIET-INDUCED OBESITY - Blunting the activity of the P2Y | 2015-09-17 |
20150259693 | TYPE II RESTRICTION MODIFICATION SYSTEM METHYLATION SUBUNIT OF ALICYCLOBACILLUS ACIDOCALDARIUS - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 2015-09-17 |
20150259694 | YEAST STRAINS FOR THE PRODUCTION OF BIOMASS ON A SUBSTRATE COMPRISING A C5 SUGAR - The present invention concerns novel | 2015-09-17 |
20150259695 | EXPRESSION SYSTEMS USING PAIRED PROMOTER INVERTED REPEATS - Pairs of plants are provided in which paired promoter-inverted-repeat constructs result in suppression of a parental phenotype in the progeny. Methods to generate and maintain such plants, and methods of use of said plants, are provided, including use of parental plants to produce or maintain sterile plants for hybrid seed production. | 2015-09-17 |
20150259696 | GUARD CELL PROMOTERS AND USES THEREOF - Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include nucleotide sequences encompassing a guard-cell-preferred promoter which drives preferential expression of gene products in guard cells. Also provided is a method for expressing a heterologous nucleotide sequence in a plant using a promoter sequence disclosed herein. | 2015-09-17 |
20150259697 | COMPOSITIONS, ORGANISMS, SYSTEMS, AND METHODS FOR EXPRESSING A GENE PRODUCT IN PLANTS - The present disclosure relates, according to some embodiments, to compositions, organisms, systems, and methods for expressing a gene product in a plant (e.g., a monocot) using a promoter operable in one or more plant tissues and/or cells. In some embodiments, an artificial nucleic acid may comprise an expression control sequence having the sequence selected from the sequence of nucleotides 1-4710 of SEQ ID NO: 1 and the sequence of nucleotides 1137-4710 of SEQ ID NO: 1, wherein the expression control sequence has stem-regulated promoter activity in at least one monocot (e.g., at least two monocots). | 2015-09-17 |
20150259698 | PLANTS HAVING ENHANCED YIELD-RELATED TRAITS AND A METHOD FOR MAKING THE SAME - The present invention provides a method for enhancing yield-related traits and/or improving various plant growth characteristics by modulating expression in a plant of a nucleic acid encoding a GRP (Growth Regulating Protein). The GRP is selected from a LOB-domain comprising protein (LOB: Lateral Organ Boundaries), herein abbreviated as LBD polypeptide, a JMJC (JUMONJI-C) polypeptide, a CKI (Casein Kinase I) polypeptide, a bHLH11-like (basic Helix-Loop-Helix 11) protein, a plant homeodomain finger-homeodomain (PHDf-HD) polypeptide, an ASR (abscisic acid-, stress-, and ripening-induced) polypeptide and/or a Squamosa promoter binding protein-like 11 (SPL11) transcription factor polypeptide. The present invention also concerns plants having modulated expression of a nucleic acid encoding a GRP, which plants have improved growth characteristics relative to corresponding wild type plants or other control plants. The invention also provides novel GRP nucleic acids and GRP polypeptides as well as constructs useful in the methods of the invention. | 2015-09-17 |
20150259699 | NUCLEOTIDE SEQUENCES AND CORRESPONDING POLYPEPTIDES CONFERRING MODIFIED PHENOTYPE CHARACTERISTICS IN PLANTS - Methods and materials for modulating low-nitrogen tolerance levels in plants are disclosed. For example, nucleic acids encoding low nitrogen tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased low-nitrogen tolerance levels and plant products produced from plants having increased low-nitrogen tolerance levels. | 2015-09-17 |
20150259700 | Transgenic Plants With RNA Interference-Mediated Resistance Against Root-Knot Nematodes - Transgenic plants that are stably resistant to the nematode | 2015-09-17 |
20150259701 | GALL WASP CONTROL AGENTS - The present invention relates to the field of doublestranded RNA (dsRNA)-mediated gene silencing in insect species. The present invention is based, in part, on the inventors' sequencing of genes from eucalyptus invasive species gall wasp pests | 2015-09-17 |
20150259702 | NOVEL BACILLUS THURINGIENSIS GENE WITH COLEOPTERAN ACTIVITY - The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of | 2015-09-17 |
20150259703 | METHOD FOR PREPARING SPECIFIC CELLS OF HUMAN-DERIVED CELLS - A method for preparing neoplastically transformed cells from human-derived cells, including the step of introducing human telomerase catalytic subunit (hTERT) gene, SV40 small T antigen (SV40ST) gene, and an oligonucleotide derived from Alu7 sequence into the human-derived cells. A method for introducing a gene for neoplastically transforming human-derived cells, including incorporating human telomerase catalytic subunit (hTERT) gene, SV40 small T antigen (SV40ST) gene, and an oligonucleotide derived from Alu7 sequence into the same or different vectors, and introducing the genes into human-derived cells therewith. The methods of the present invention can be utilized upon induction of neoplastic transformation to various human normal cells in order to elucidate mechanisms for onset of cancer, so that the method can be effectively utilized in the search of a new drug discovery target molecule. | 2015-09-17 |
20150259704 | RNA-Guided Human Genome Engineering - A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner. | 2015-09-17 |
20150259705 | RHODOBACTER FOR PREPARING TERPENOIDS - The invention relates to a | 2015-09-17 |
20150259706 | GROWTH METHOD FOR MICROBE AND BIOETHANOL PRODUCTION METHOD - The present invention provides a growth method for a microbe which can enhance the growth of the microbe without removing growth inhibitors from a saccharified solution. The growth method for a microbe comprises the steps of: obtaining a mixed saccharified solution by diluting the saccharified solution containing a growth inhibitor which inhibits the growth of the microbe with a sugar solution having a smaller growth inhibitor concentration than that of the saccharified solution such that the growth inhibitor concentration is decreased; and allowing the microbe to grow by adding the microbe to the mixed saccharified solution. | 2015-09-17 |
20150259707 | Metabolically Enhanced Cyanobacterial Cell for the Production of Ethanol - A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn | 2015-09-17 |
20150259708 | Metabolically Enhanced Cyanobacterial Cell for the Production of Ethanol - A metabolically enhanced cyanobacterial cell for the production of ethanol is provided. The metabolically enhanced cyanobacterial cell for the production of ethanol comprises at least one recombinant gene encoding a pyruvate decarboxylase enzyme (Pdc) converting pyruvate to acetaldehyde, and at least one recombinant gene encoding a first Zn | 2015-09-17 |
20150259709 | PROCESSES FOR PRODUCING FLUFF PULP AND ETHANOL FROM SUGARCANE - The disclosure provides a process for producing fluff pulp and ethanol from sugarcane bagasse or straw, comprising: fractionating the feedstock in the presence of an acid catalyst, a solvent for lignin, and water, to generate a solid/liquid slurry comprising cellulose-rich solids, hemicelluloses, and lignin; separating the solid/liquid slurry into a solid stream and a liquid stream; further treating the cellulose-rich solids to produce fluff pulp; hydrolyzing the hemicelluloses to generate hemicellulose monomers; and fermenting at least a portion of the hemicellulose monomers to cellulosic ethanol. Lignin is removed from the process during one or more steps and combusted to provide energy for process requirements. The process is integrated with, and provides energy to, a first-generation process that ferments sugarcane-derived sucrose to first-generation ethanol. Similar processes are possible with energy cane, corn, and other crops. | 2015-09-17 |
20150259710 | DECARBOXYLASE PROTEINS WITH HIGH KETO-ISOVALERATE DECARBOXYLASE ACTIVITY - The present invention relates to recombinant microorganisms comprising an isobutanol producing metabolic pathway and methods of using said recombinant microorganisms to produce isobutanol. In various aspects of the invention, the recombinant microorganisms may comprise at least one nucleic acid molecule encoding a polypeptide with keto-isovalerate decarboxylase (KIVD) activity, wherein said polypeptide is at least about 65%, 70%, 75%, 80%, 85%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, or 99% identical to a polypeptide selected from SEQ ID NOs: 1-214. Also provided are modified decarboxylases exhibiting an improved ability to utilize α-ketoisovalerate as a substrate in various beneficial enzymatic conversions. | 2015-09-17 |
20150259711 | METHODS OF PRODUCING ACETOIN AND 2,3-BUTANEDIOL USING PHOTOSYNTHETIC MICROORGANISMS - The present disclosure provides recombinant cyanobacteria having acetolactate synthase activity and acetolactate decarboxylase activity, as well as recombinant cyanobacteria having acetolactate synthase activity, acetolactate decarboxylase activity, and secondary alcohol dehydrogenase activity. Methods for the production of the recombinant cyanobacteria, as well as methods for use thereof for production of acetoin and 2,3-butanediol from carbon dioxide and light are also provided. | 2015-09-17 |
20150259712 | FATTY ACIDS WITH MG TRANSPORTER AND MG - Methods of improving fatty acid production in bacteria, yeast, algae, and various other microbes are presented, by either supplementing the medium with millimolar amounts of magnesium, by overexpressing a magnesium transporter, or both. | 2015-09-17 |
20150259713 | PROCESS FOR PREPARING SEBACIC ACID - A process for preparing sebacic acid by reacting in a first step (i) linoleic acid with water catalyzed by an oleate hydratase to form 10-hydroxy-12-octadecenoic acid, in a second step (ii) pyrolysing the 10-hydroxy-12-octadecenoic acid to 1-octene and 10-oxo-decanoic acid and in a third step (iii) oxidizing the 10-oxo-decanoic acid to sebacic acid. | 2015-09-17 |
20150259714 | SYSTEMS AND METHODS FOR PRODUCTION OF MIXED FATTY ESTERS - Disclosed herein are various embodiments regarding the production of mixed fatty esters. Disclosed herein are various embodiments regarding the use of mixed alcohol compositions for the production of fatty esters. | 2015-09-17 |
20150259715 | L-Lysine Hydroxylase and Production Method for Hydroxy-L-Lysine and Hydroxy-L-Pipecolic Acid Using Same - The present invention aims to provide a method for efficiently producing hydroxy-L-lysine. The present invention provides a method for producing hydroxy-L-lysine, the method comprising allowing 2-oxoglutarate-dependent L-lysine hydroxylase, a cell containing 2-oxoglutarate-dependent L-lysine hydroxylase, a processed product of the cell, and/or a culture broth obtained by culturing the cell, to act on L-lysine to produce hydroxy-L-lysine represented by the following General Formula (I) (wherein each of R | 2015-09-17 |
20150259716 | Method for Producing Basic Substance - A method for producing a basic substance by fermentation comprising culturing a microorganism having an ability to produce the basic substance in a liquid medium contained in a fermentation tank to produce and accumulate the basic substance in the medium, wherein amount of sulfate and/or chloride ions used as counter ions of the basic substance is reduced by adjusting total ammonia concentration in the medium to be within a specific concentration range during at least a part of the total period of culture process. | 2015-09-17 |
20150259717 | Method for Producing an Acidic Substance Having a Carboxyl Group - An acidic substance having a carboxyl group is produced by culturing in a medium a microorganism which has been modified to enhance expression of the ybjL gene, and collecting the acidic substance having a carboxyl group from the medium. | 2015-09-17 |
20150259718 | LOV-D ACYLTRANSFERASE MEDIATED ACYLATION - Methods for the improved acylation of chemical substrates using LovD acyltransferases, thioesters having acyl groups, and (i) thiol scavengers and/or (ii) precipitating agents are presented. An improved method for the production of simvastatin using (i) activated charcoal as a thiol scavenger and/or (ii) ammonium hydroxide as a precipitating agent is also presented. | 2015-09-17 |
20150259719 | Enzymatic Hydrolysis of Old Corrugated Cardboard (OCC) Fines from Recycled Linerboard Mill Waste Rejects - A significant fraction of short fibers (fines) is produced while recycling Old Corrugated Containerboards (OCC), which are usually rejected as solid waste stream, requiring landfilling and posing environmental problems. The major component of these fines rejects are primarily cellulose that can be hydrolyzed into sugars for possible fermentation into biofuels, bioplastics or other sugar based products. Use of fines also offers benefits such as negative costs and production of fermentable sugars without requiring complex pretreatment processes, now required to hydrolyze and eliminate inhibitors from hydrolyzate. Enzymatic hydrolysis of reject fines from a recycled OCC mill, employing different strains of cellulases, were investigated. Fillers (up to 30 mass %) in the fines increases the required dosage of enzymes and costs. Enzyme loading can be lowered by addition of surfactants to reduce their inhibitory activity. The nonionic surfactant Triton X-80 improved hydrolysis yields by up to 10 percent points. | 2015-09-17 |
20150259720 | METHODS FOR PRODUCING RECOMBINANT GLYCOPROTEINS WITH MODIFIED GLYCOSYLATION - Genetically engineered host animal cells capable of producing glycoproteins having modified glycosylation patterns, e.g., defucosylation and/or monoglycosylation. Such host animal cells can be engineered to express fucosidase, endoglycosidase or both. | 2015-09-17 |
20150259721 | CELL LINES - There is provided inter alia a process for stabilizing a eukaryotic cell line which expresses PylRS and tRNAPyl and which is suitable for incorporation of a gene encoding a target protein containing one or more non-natural amino acids encoded by a nonsense codon which comprises culturing said cell line under conditions in which the adverse effect of tRNAPyl expression on cell viability and/or cell growth is reduced or eliminated. | 2015-09-17 |
20150259722 | BIOSENSOR COMPOSITIONS AND METHODS OF THEIR USE - Embodiments of the present disclosure provide for biosensors that include a material such as polydiacetylene (PDA) material, where the material is used for detection of a microbe or microbial product present in a fluid present in the container. Embodiments of the present disclosure provide for containers, or structures used in conjunction with the containers, that include a polydiacetylene (PDA) material, where the PDA material is used for detection of a microbe or microbial product present in a fluid present in the container. In an embodiment, a change of PDA color (e.g., blue to red) indicates detection of the microbe or microbial product in the fluid within the container. In an embodiment, the PDA material can be selected and/or the container or structure designed so that only certain types of microbes can be detected or so that a plurality of types of microbes is detected. | 2015-09-17 |
20150259723 | Firmware Design for Area and Location Data Management of Biological Air Samples Collected on Media Plates - Provided herein are methods and devices that allow for efficient management of many different sampling locations within a facility. A method for operating a biological sampler is described, such as by sampling an environment at a sampling position with the biological sampler and associating the sampling position with a unique identifier, wherein the unique identifier comprises an area and a location. Also provided are associated devices for carrying out the methods. | 2015-09-17 |
20150259724 | NANOPORE STOCHASTIC SENSING OF BIOMARKERS - A method and system for sensing or characterizing a biomarker, such as a proteolytic enzyme or nucleic acid. The system comprises a nanopore sensor to determine a current modulation of a sample including a biomarker, and a predetermined substrate or nucleic acid probe current modulation signature for comparison to a current signature from the nanopore sensor. The nanopore sensor includes a nanopore membrane between two fluid compartments, and a power supply in electrical contact with the membrane to provide an electric potential difference between the fluid compartments. A detector is used to detect an electrical current through the nanopore as the polypeptide substrate, or components thereof, transits the nanopore under an applied electric potential difference between the first and second fluid compartments. The result is a rapid, label-free method for the sensitive and accurate measurement of biomarker activity by real-time monitoring of the ionic current modulations arising from the substrate peptide-protease interactions or nucleic acid hybridization in the nanopore. | 2015-09-17 |
20150259725 | ANALYSIS OF SMALL RNA - A method for modifying a strand of RNA at the 3′ end, includes contacting the strand with a RNA 2′-O-methyltransferase in the presence of a co-factor, under conditions which allow for the transfer by the RNA 2′-O-methyltransferase of a part of the co-factor onto the 3′ end of the RNA strand to form a modified RNA strand, wherein the strand of RNA is included in a duplex, and wherein the part of the co-factor transferred includes a reporter group or a functional group. | 2015-09-17 |
20150259726 | Multiplex Targeted Amplification Using Flap Nuclease - Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5′ or 3′ flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5′ and 3′ flaps are generated. The flaps are cleaved using 5′ or 3′ flap endonucleases or 3′ to 5′ exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified. | 2015-09-17 |
20150259727 | ALLELIC DISCRIMINATION ASSAYS FOR MRSA STRAINS - The present invention provides assays, methods and kits that may be used to detect and differentiate MRSA isolates, e.g., USA100, USA300 and USA600 strains. | 2015-09-17 |
20150259728 | METHODS AND SYSTEMS FOR MICROBIOME CHARACTERIZATION, MONITORING AND TREATMENT - The present disclosure provides methods for profiling a microbiome and therapeutic compositions for treatment. Additionally, the methods, systems, compositions and kits provided herein are directed to assessing or predicting health status in a subject. Some of the embodiments include generating a report. | 2015-09-17 |
20150259729 | METHODS OF DETECTING MULTI-DRUG RESISTANT ORGANISMS - The present invention provide methods using genes associated with multi-drug resistance for rapidly detecting a patient colonized or infected with an multi-drug resistant organism and administrating the appropriate precautions and/or treatment. | 2015-09-17 |
20150259730 | MICROFLUIDIC CHIP, OPERATING SYSTEM AND OPERATING METHOD OF THE MICROFLUIDIC CHIP FOR FLUORESCENCE IN SITU HYBRIDIZATION - A microfluidic chip is provided, including: a plurality of first storage tanks and a plurality of second storage tanks that respectively store different solutions for a preprocess and hybridization process of the fluorescence in situ hybridization; a transmission unit adjacent to the first storage tanks; a reaction unit adjacent to the second storage tanks for a biological target to be placed thereon; a plurality of first valves disposed between the first storage tanks and the transmission unit; and a plurality of second valves disposed between the second storage tanks and the reaction unit, wherein the first valves and the second valves are opened in a predetermined sequence so as to transmit the solutions of the first storage tanks and the second storage tanks to the reaction unit, such that the solutions perform the preprocess and hybridization process of the fluorescence in situ hybridization to the biological target in sequence. | 2015-09-17 |
20150259731 | DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES COMPRISING CHARGE MASS TAGS - Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a DNA polymerase complex so that polymerization may not be influenced. | 2015-09-17 |
20150259732 | NUCLEIC ACID ANALYSIS KIT AND NUCLEIC ACID ANALYSIS METHOD - A number of samples provided for analysis are identified correctly by making use of smaller kinds of nucleic acids. Provided is a nucleic acid analysis kit having a plurality of supports that respectively retain identifier probes containing a nucleic acid and support specimens. The identifier probes contains at least one kind of a nucleic acid having a known base sequence, differ in at least one of the kind and amount of the nucleic acid for each of the supports, and are retained by the supports so as to be mixed with the specimens, respectively. | 2015-09-17 |
20150259733 | Methods and Compositions for Amplification and Sequencing of Difficult DNA Templates - This disclosure provides methods and compositions for amplification and sequencing of DNA templates, comprising at least two of: 2′-deoxyinosine-5′ triphosphate, 5-propynyl-2′-deoxycytidine-5′-triphosphate, and 8-oxo-2′-deoxyguanosine-5′-triphosphate. Incorporation of these promoting nucleotides into amplification and sequencing reactions improves the amplification and sequencing of difficult-to-sequence DNA regions such as a GC rich regions or GT rich regions; repetitive sequences, including dinucleotide, trinucleotide, direct, inverted, Alu, poly A or poly T repeats; and hairpin or other secondary structures. | 2015-09-17 |
20150259734 | LARGE-SCALE BIOMOLECULAR ANALYSIS WITH SSEQUENCE TAGS - The invention is directed to sequence-based profiling of populations of nucleic acids by multiplex amplification and attachment of one or more sequence tags to target nucleic acids anchor copies thereof followed by high-throughput sequencing of the amplification product. In some embodiments, the invention includes successive steps of primer extension, removal of unextended primers and addition of new primers either for amplification (for example by PRC) or for additional primer extension. Some embodiments of the invention are directed to minimal residual disease (MRD) analysis of patients being treated for cancer. Sequence tags incorporated into sequence reads provide an efficient means for determining clonotypes and at the same time provide a convenient means for detecting carry-over contamination from other samples of the same patient or from samples of a different patient which were tested in the same laboratory. | 2015-09-17 |
20150259735 | POLYMER COATINGS - The present disclosure relates to polymer coatings covalently attached to the surface of a substrate and the preparation of the polymer coatings, such as poly(N-(5-azidoacetamidylpentyl)acrylamide-co-acrylamide) (PAZAM), in the formation and manipulation of substrates, such as molecular arrays and flow cells. The present disclosure also relates to methods of preparing a substrate surface by using beads coated with a covalently attached polymer, such as PAZAM, and the method of determining a nucleotide sequence of a polynucleotide attached to a substrate surface described herein. | 2015-09-17 |
20150259736 | LINKING SEQUENCE READS USING PAIRED CODE TAGS - Artificial transposon sequences having code tags and target nucleic acids containing such sequences. Methods for making artificial transposons and for using their properties to analyze target nucleic acids. | 2015-09-17 |
20150259737 | METHOD FOR NUCLEOTIDE DETECTION - A method of inhibiting light-induced degradation of nucleic acids includes irradiating a portion of the nucleic acids in the presence of a detection solution comprising a polyphenolic compound. A method of detecting a nucleic acid having a fluorescent tag includes irradiating at least a portion of the nucleic acid with light of a suitable wavelength to induce a fluorescence emission and detecting the fluorescence emission. Optionally, the polyphenolic compound is gallic acid, a lower alkyl ester thereof, or mixtures thereof. A kit includes one or more nucleotides, an enzyme capable of catalyzing incorporation of the nucleotides into a nucleic acid strand and a polyphenolic compound suitable for preparing a detection solution. | 2015-09-17 |