| 36th week of 2010 patent applcation highlights part 37 |
| Patent application number | Title | Published |
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| 20100227309 | METHOD FOR QUANTITATIVELY DETERMINING LDL CHOLESTEROLS - A method for quantitatively determining LDL cholesterol, including the steps of adding to serum a surfactant selected from among polyoxyethylenealkylene phenyl ethers and polyoxyethylenealkylene tribenzylphenyl ethers and a cholesterol-assaying enzyme reagent so as to preferentially react cholestrols in high density- and very low density-cholesterols among lipoproteins, and subsequently determining the amount of cholesterol that reacts thereafter. This method can eliminate the necessity for pretreatments such as centrifugation and electrophoresis, enables the quantitative determination to be conducted in an efficient, simple manner, and can be applied to various automatic analyzers. | 2010-09-09 |
| 20100227310 | FLOW CYTOMETRY METHODS AND IMMUNODIAGNOSTICS WITH MASS SENSITIVE READOUT - Mass cytometry method. In one aspect, the method includes providing a sample having at least one cell type and mixing the sample with material such as nanoparticles functionalized with affinity molecules for the at least one cell type. The sample is transported through a suspended microchannel resonator to record a mass histogram and a cell count for the at least one cell type is determined from the histogram. | 2010-09-09 |
| 20100227311 | TISSUE CULTURE SYSTEM FOR PRODUCTION OF HEPATITIS C VIRUS - A tissue culture system for production of infectious hepatitis C virus is described. In particular, the invention provides recombinant monocistronic and bicistronic genomic constructs for production of virus, including constructs for production of wild-type HCV type 2 | 2010-09-09 |
| 20100227312 | Particle Adhesion Assay for Microfluidic Bifurcations - A method for characterizing particle adhesion in microfluidic bifurcations and junctions comprises at least one idealized bifurcation or junction. Multiple bifurcations and/or junctions can be combined on a single microfluidic chip to create microfluidic networks configured for assays specifically to characterize particle interactions at junctions or to screen particles for desired interactions with microfluidic bifurcations and/or junctions. | 2010-09-09 |
| 20100227313 | Methods and Compositions for Determining Altered Susceptibility of HIV-1 to Protease Inhibitor Treatment - This invention relates to methods for determining altered susceptibility of HIV-I viruses to protease inhibitors (PIs) based on the viral genotypes. The methods generally comprise detecting, in a gene encoding protease and/or gag of the HIV-I, the presence of mutations correlated with altered susceptibility to amprenavir and/or darunavir. | 2010-09-09 |
| 20100227314 | METHOD OF IDENTIFICATION OF GENOTYPE AND SUBTYPE OF HEPATITIS C VIRUS ON A BIOLOGICAL MICROCHIP - The invention relates to molecular biology, virology and medicine and provides a method for identifying a genotype and a subtype of Hepatitis C virus (HCV) on the basis of the analysis of an HCV genome NS5B region using a differentiating biochip. The method of the present invention is based on a two-step PCR, with a fluorescent labeled, preferably single-stranded, NS5B region fragment obtaining, followed by the hybridization of this fragment on a biochip comprising a set of specific discriminating oligonucleotides. HCV genotype and subtype identification is carried out by defining the specific sequences of the segments of the NS5B region fragment. The invention allows one to conduct an assay precisely from a clinical specimen, to determine 6 genotypes and 36 subtypes of hepatitis C virus, including the most virulent and drug resistant forms, and to reduce the cost of assay. Also, the invention deals with a biochip, a design method and a set of oligonucleotide probes usable under the implementation of the method. | 2010-09-09 |
| 20100227315 | Biosensor Using Whispering Gallery Modes in Microspheres - A biosensor for detecting the presence of a target analyte is disclosed. The biosensor is formed from microspheroidal particles which have had a binding partner for the target analyte immobilized on their surfaces. The binding partners may be nucleotides; peptides, proteins, enzymes, antibodies and so on. When the analyte binds to its partner, the whispering gallery mode (WGM) profiles of the microspheroidal particles change such that the profile peaks undergo a red-or blue-shift. The immobilised binding partners may include fluorophores and the like so that they emit fluorescence, phosphorescence, incandescence and the like. These fluorophores may take the form of a nanocrystal or quantum dot. | 2010-09-09 |
| 20100227316 | LUMINESCENSE MEASURING APPARATUS AND LUMINESCENSE MEASURING METHOD - There is provided a novel luminescence measuring apparatus that enables measuring a luminescence amount or a luminescence intensity from plural living biological samples, such as tissues, cells, and biological individuals, into which a gene expressing a luminescent protein has been introduced, individually for the respective biological samples. The inventive luminescence measuring apparatus comprises an image acquisition portion that acquires a luminescence image of biological samples; a measurement region determination portion that determines at least one measurement region in the luminescence image; and a luminescence measurement portion that measures the luminescence amount or luminescence intensity in the determined measurement region; thereby measuring the luminescence amount or luminescence intensity individually by the biological sample(s) included in the measurement region. Irrespective of an efficiency of transfer of a luminescent protein gene into a cell, etc., it becomes possible to execute a luminescence measurement successfully while observing only biological samples having a gene expression. | 2010-09-09 |
| 20100227317 | Method for the Molecular Diagnosis of Prostate Cancer and Kit for Implementing Same - The invention relates to a method for the molecular diagnosis of prostate cancer, comprising the in vitro analysis of the overexpression or underexpression of combinations of genes that can distinguish, with high statistical significance, tumorous prostate samples from non-tumorous prostate samples. The invention also relates to a kit for the molecular diagnosis of prostate cancer, which can perform the above-mentioned detection. | 2010-09-09 |
| 20100227318 | METHOD FOR CONTROLLING THE AMOUNT OF GENE PRODUCT, AND AGENT FOR CONTROLLING THE AMOUNT OF GENE PRODUCT - The invention relates to a method of intracellularly controlling amounts of gene products, which can increase an amount of gene product intracellularly, comprising a step of introducing into the cell a substance having a sequence complementary to the base sequence of mRNA corresponding to the gene product, its precursor or another substance which can have equivalent action in the cell. | 2010-09-09 |
| 20100227319 | USE OF RUNX3 AND MIR-532-5P AS CANCER MARKERS AND THERAPEUTIC TARGETS - The invention relates to methods for cancer diagnosis, prognosis, and treatment based on the expression or activity levels of RUNX3 and miR-532-5p. Also disclosed is a method of reducing the inhibition of RUNX3 by miR-532-5p with an agent that interferes with the interaction between RUNX3 and miR-532-5p transcripts. | 2010-09-09 |
| 20100227320 | NUCLEIC ACID DETECTION - This invention provides methods and kits for enriching and/or detecting a nucleic acid with at least one variant nucleotide from a nucleic acid population in a sample. Methods employ the use of enriching primers and bridge-probes for enriching and detecting target nucleic acids. Extension of the enriching primer permits amplification of the target nucleic acid having the variant nucleotide. | 2010-09-09 |
| 20100227321 | METHODS AND DEVICES FOR NUCLEIC ACID SEQUENCE DETERMINATION - Methods of the invention comprise methods and devices for nucleic acid sequence determination. Generally, the invention relates to preparing a substrate for sequencing a target nucleic acid | 2010-09-09 |
| 20100227322 | Detection of Bordetella - The invention provides methods to detect | 2010-09-09 |
| 20100227323 | MICROCHANNEL DETECTION DEVICE AND USE THEREOF - The present invention relates to a device and methods for detecting or quantifying an analyte in a test sample. The device includes a substrate defining one or more microchannels and having a reaction region in a first portion of the one or more microchannels, wherein the reaction region contains a first binding element selected to bind with a first portion of the analyte. The device also includes a detection region in fluid communication with the reaction region. The detection region includes a second binding element selected to immobilize the analyte within the detection region. Methods of detecting or quantifying an analyte in a test sample using the device of the present invention are also disclosed. A method for coating a polymer with a gold layer is also disclosed. | 2010-09-09 |
| 20100227324 | MEASUREMENT OF ARYLESTERASE ENZYMATIC ACTIVITY AND ASSESSMENT OF GENETIC POLYMORPHISMS LOCATED IN THE PON1 GENE AS A DIAGNOSTIC TOOL IN AUTISM-SPECTRUM DISORDERS - The present invention concerns a method for detecting the presence of or predisposition to autism, an autism spectrum disorder, or an autism-associated disorder in a subject, the method comprising measuring an arylesterase enzymatic activity in a sample from the subject, optionally combined with the determination of alleles of PON1 polymorphisms. | 2010-09-09 |
| 20100227325 | METHODS OF DETECTING SEPSIS - Methods of sepsis in a sample from a patient are provided. Methods of detecting changes in expression of one or more microRNAs associated with sepsis are also provided. Compositions and kits are also provided. | 2010-09-09 |
| 20100227326 | Detection System - A method for detecting the presence of a target nucleic acid sequence in a sample, said method comprising: performing nucleic acid amplification on the sample in the presence of (a) a DNA duplex binding agent, (b) a nucleic acid polymerase and (c) a reagent comprising an amplification primer which can hybridise to said target sequence when in single stranded form and which is connected at its 5′ end to a probe which carries a label by way of a chemical linking group, said labelled probe being of a sequence which is similar to that of the said target nucleic acid sequence, such that it can hybridise to a complementary region in an amplification product, and wherein the label is able to absorb fluorescence from or donate fluorescent energy to the DNA duplex binding agent; and monitoring fluorescence of said sample. | 2010-09-09 |
| 20100227327 | METHODS AND COMPOSITIONS FOR CONTINUOUS SINGLE-MOLECULE NUCLEIC ACID SEQUENCING BY SYNTHESIS WITH FLUOROGENIC NUCLEOTIDES - Disclosed herein are methods and compositions for continuous single-molecule nucleic acid sequencing by synthesis with fluorogenic nucleotides. | 2010-09-09 |
| 20100227328 | DNAZYMES AND SENSORS INCORPORATING THE SAME - An arsenic ion active DNAzyme includes a nucleotide sequence, which has a base sequence selected from ATCTCCTCCTGTTC (SEQ ID NO: 62), ATCTGCTCCTGTTC (SEQ ID NO: 63), ATCTCCTCATGTTC (SEQ ID NO: 64), ATCTCCTCTTGTTC (SEQ ID NO: 65), ATCTCCAACCTGTTC (SEQ ID NO: 66), and CCGTAGCGCAAAT (SEQ ID NO: 67). A mercury ion active DNAzyme includes a nucleotide sequence, which has a base sequence selected from AATTCCGTAGGTCCAGTG (SEQ ID NO: 68), AATTCCGTCGGTCCAGTG (SEQ ID NO: 69), AATTCCGCCGGTCCAGTG (SEQ ID NO: 70), GGTTCCGAGTCTCGCGTG (SEQ ID NO: 71), and CGTTCAAAAGGGGCACTG (SEQ ID NO: 72). Sensors incorporating the respective DNAzymes are also disclosed. | 2010-09-09 |
| 20100227329 | TOOLS AND METHODS FOR GENETIC TESTS USING NEXT GENERATION SEQUENCING - The present invention provides tools and methods for use in genetic tests involving high performant sequencing techniques. More particularly, the invention provides a robust multiplex PCR method wherein the respective primers for amplifying the different amplicons are physically isolated from one another. The invention further provides quality control methods allowing a stringent monitoring of genetic tests carried out according to the present invention. | 2010-09-09 |
| 20100227330 | METHODS FOR DIAGNOSING EPISODIC MOVEMENT DISORDERS AND RELATED CONDITIONS - The present invention provides compositions and methods for research, diagnostic, drug screening, and therapeutic applications related to paroxysmal dystonic choreoathetosis and related conditions. In particular, the present invention provides mutations in the myofibrillogenesis regulator 1 (MR-1) gene associated with such conditions. | 2010-09-09 |
| 20100227331 | DETECTION OF IMMOBILIZED NUCLEIC ACID - The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures. | 2010-09-09 |
| 20100227332 | Method for selecting antimicrobial agent and utilization thereof - The present invention provides for a method for selecting an antimicrobial agent, which comprises a microbe analyzing step in which microbes in the microbial biota of a sample are analyzed based on base sequence of DNA and an antimicrobial agent selecting step comprising performing search in a data base storing data for industrial antimicrobial agents applicable to the analyzed microbes recited at effective concentrations, picking up industrial antimicrobial agents effective for the microbial biota, dominant microbe or specific microbe(s) in the sample analyzed in the above microbe analyzing step and selecting among the picked up ones an industrial antimicrobial agent, which method permits to select an antimicrobial agent that is optimum for the treatment in a simple and assured manner within a brief time and to grasp whether the effect of the antimicrobial agent is revealed or not in a simple and assured manner within a brief time, by utilizing this selecting method for the selection of antimicrobial agent, for example, in an antimicrobial treating, in monitoring of the antimicrobial effect, in slime control in paper manufacturing process courses and in microbe inhibition in paper manufacturing process courses, whereby troubles caused by growth of microbes can be prevented beforehand. | 2010-09-09 |
| 20100227333 | METHOD OF DETECTING INFECTION WITH UROGENITAL MYCOPLASMAS IN HUMANS AND A KIT FOR DIAGNOSING SAME - A method and a kit are provided for detecting an infection by mycoplasma, particularly an urogenital infection in humans. The presence of specific anti-mycoplasma antibodies in a biological sample of a diagnosed subject is detected by reaction with an immobilized mixture of various antigenic determinants associated with a variety of pathological states. | 2010-09-09 |
| 20100227334 | Reaction sensing in living cells - Chemical reactions occurring within a living cell are measured in a manner that does not affect the viability of the cell or the reaction under study. In one embodiment, one or more sensors are introduced into the cell and/or covalently associated with the exterior cell membrane. The sensor(s) emit an observable signal indicating a value of a parameter associated with the chemical reaction, e.g., the concentration of a reaction product. Because cell viability is not compromised, the cell may be stimulated (e.g., by subjection to an agonist or antagonist, a pathogen, a pharmaceutical compound, or a potential toxin) so as to affect the reaction under study. | 2010-09-09 |
| 20100227335 | MATRIX METALLOPROTEINASE-7 (MMP-7) MONOCLONAL ANTIBODIES AND METHODS FOR THEIR USE IN THE DETECTION OF OVARIAN CANCER - Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to MMP-7. Monoclonal antibodies having the binding characteristics of an MMP-7 antibody of the invention and monoclonal antibodies that bind to an MMP-7 epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce an MMP-7 monoclonal antibody of the invention are also disclosed herein. The compositions find use in diagnostic methods as well as in screening methods for identifying patients having an increased likelihood of having ovarian cancer. Kits comprising one or more of the disclosed MMP-7 monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for an MMP-7 epitope of a disclosed monoclonal MMP-7 antibody and methods of using these polypeptides in the production of MMP-7 antibodies are also encompassed by the present invention. | 2010-09-09 |
| 20100227336 | METHOD FOR THE MEASURE OF MOLECULAR INTERACTIONS BY MEASUREMENT OF THE LIGHT REFLECTED BY PLANAR SURFACES - Procedure for the quantitative determination of interactions of ligands with receptors adsorbed or immobilized on the surface of a solid material which can be functionalized, transparent and with low refractive index, by direct measure of the reflection of light. | 2010-09-09 |
| 20100227337 | Method for Evaluating Risk in Multiple Sclerosis - The invention relates to methods and reagents for diagnosing and assessing the prognosis of multiple sclerosis. | 2010-09-09 |
| 20100227338 | Method and Apparatus for Rapid Detection and Identification of Live Microorganisms Immobilized On Permeable Membrane by Antibodies - An apparatus and method is provided for the rapid detection, identification and/or enumeration of one or more live target microorganisms growing on solid nutrient media or filtration membrane. Microcolonies or colonies attach to a porous transfer permeable membrane loaded with immobilized antibodies specific for target microorganisms. Non-specific cells are washed out of the permeable membrane and the membrane then is placed on a container containing a chromogenic and/or a fluorogenic substrate and incubated to allow coloration and subsequent identification and enumeration of color spots of targeted microorganisms and patterns of microcolonies or colonies. | 2010-09-09 |
| 20100227339 | DIFFERENTIAL IMMUNOASSAY FOR PRRS VACCINE ANTIBODY - The present invention relates to immunoassays for serologically differentiating animals naturally infected with PRRS virus from animals vaccinated against PRRS. The immunoassays provide detection of at least a portion of the N terminal region of the 2b portion of PRRSV. The immunoassay is preferably an enzyme-linked immunosorbent assay (ELISA). | 2010-09-09 |
| 20100227340 | METHOD OF ANALYSIS, DETECTION AND CORRECTION OF FOOD INTOLERANCE IN HUMANS - A method of detection and correction of latent food intolerance (LFI) in humans is described. The method allows, through utilizing a dynamic analysis of a specific test for LFI, assessing the degree of food intolerance and devising an individualized diet that excludes food items which cause latent food intolerance, as well as food items which cause classic allergic reactions. Thus, this method promotes health throughout an individual's life by avoiding and correcting the negative effects of food items that act as immune antagonists. | 2010-09-09 |
| 20100227341 | Diagnosing Pneumococcal Pneumonia - Compositions and methods for eliciting an immune response against | 2010-09-09 |
| 20100227342 | DIAGNOSIS OF PREECLAMPSIA - The present invention provides methods and compositions related to the detection and/or monitoring of the levels of angiogenic factors, specifically VEGF, PlGF and sFlt-1, in urine samples obtained from pregnant women and the effects of such levels on the risk of developing complications of pregnancy, including hypertensive disorders such as preeclampsia, in the first, second, and/or third trimester of pregnancy. The present invention also provides kits for identifying and screening patients at risk of developing a complication of pregnancy, such as preeclampsia. | 2010-09-09 |
| 20100227343 | GLYCODELIN MONOCLONAL ANTIBODIES AND METHODS FOR THEIR USE IN THE DETECTION OF OVARIAN CANCER - Compositions and methods for diagnosing ovarian cancer in a patient and for identifying patients with an increased likelihood of having ovarian cancer are provided. The compositions include novel monoclonal antibodies, and variants and fragments thereof, that specifically bind to glycodelin. Monoclonal antibodies having the binding characteristics of a glycodelin antibody of the invention and monoclonal antibodies that bind to a glycodelin epitope of a disclosed antibody are further provided. Hybridoma cell lines that produce a glycodelin monoclonal antibody of the invention are also disclosed herein. The compositions find use in diagnostic methods as well as in screening methods for identifying patients having an increased likelihood of having ovarian cancer. Kits comprising one or more of the disclosed glycodelin monoclonal antibodies and for practicing the methods of the invention are further provided. Polypeptides comprising the amino acid sequence for a glycodelin epitope of a disclosed monoclonal glycodelin antibody and methods of using these polypeptides in the production of glycodelin antibodies are also encompassed by the present invention. | 2010-09-09 |
| 20100227344 | METHODS FOR MEASURING ADP - This invention relates to assays for detecting and measuring ADP. In particular, this invention provides homogeneous luminescent assays that detect ADP generation and measures ADP accumulation based on enzymatic coupling reactions. The assays of the present invention can be applied to all types of kinases and other ADP-generating enzymes, are antibody free, beads free, radioisotope free, and compatible with commonly used kinase buffers. | 2010-09-09 |
| 20100227345 | Enzyme containing liquid and delivery system for detection of analytes on surfaces - An aqueous enzyme based sensor comprising one or more enzymes and at least one indicator compound capable of indicating a reaction of at least one of the enzymes with at least one target analyte, wherein the enzyme and the indicator are capable of being delivered, preferably by being sprayed, to a surface having the target analyte without sampling the surface. An aqueous enzyme based sensor is provided that is capable of detecting an enzyme specific substrate as the analyte. This aqueous enzyme based sensor further comprises of one or more enzyme specific substrates in which the sensor detects enzyme inhibition. The aqueous enzyme based sensor optionally comprises one or more of a light scattering additive(s), an adhesive polymer(s), a protein stabilizer(s), a protein stabilizing sugar(s), a surfactant(s), and a solvent(s), and combinations thereof. A dispensing system and a method of detecting a target chemical on a surface without sampling the surface is provided. | 2010-09-09 |
| 20100227346 | Activation Mixture - The present invention relates to an activation mixture comprising factor-deficient substrate plasma, an activator, and a phospholipid. The present invention also relates to a method for preparing, and the use of, an activation mixture. The present invention further relates to an assay method for determining the amount of blood coagulation factor in a test sample and a kit for performing the assay method described therein. | 2010-09-09 |
| 20100227347 | DIAGNOSTIC TEST CALIBRATION ADJUSTMENT METHOD - A method is provided for adjusting either the signal measured or activity calculated by an instrument or a predefined calibration curve for a medical diagnostic test, performed on an instrument with pre-calibrated reagents. A calibration adjuster for a blood clotting diagnostic test is further provided. | 2010-09-09 |
| 20100227348 | METHOD AND DEVICE FOR DETERMINING THE CONCENTRATION OF AN ANALYTE USING FLUORESCENCE MEASUREMENT - A method is presented for determining the concentration of an analyte in a liquid sample using a reagent system. The reaction of the sample with the reagent system results in a change of the quantity of a fluorophore, the quantity of the fluorophore correlating with the concentration of the analyte, a characteristic measurement variable for the quantity of the fluorophore being measured, and the concentration of the analyte being determined using an evaluation algorithm on the basis of the measurement variable. The reagent system is integrated in an analysis system and the fluorescence lifetime of the fluorophore is used in the ascertainment of the concentration of the analyte. | 2010-09-09 |
| 20100227349 | USE OF LYMPHOCYTES TO MEASURE ANTHRAX LETHAL TOXIN ACTIVITY - It is disclosed herein that isolated lymphocytes, such as human B-cells and CD4 | 2010-09-09 |
| 20100227350 | Method of Screening Substance Useful in Treating Disease With the Use of GPR40 and Phospholipase - The present invention relates to a screening method for determining whether a substance of interest is a substance which alters GPR40-mediated cell stimulating activities, comprising using a substance of interest, a biomembrane containing GPR40, or cells containing said biomembrane, and phospholipase or salts thereof. According to the present invention, substances involved in insulin secretion can be screened. In addition, according to the present invention, substance useful for the prevention or treatment of diabetes, diabetic complications and degenerative diseases, hyperglycemia, polyuria, ketonemia, acidosis, insulin resistance, impaired glucose tolerance, neurodegenerative diseases, insulinoma, cancers, hyperinsulinemia, hyperglyceridemia, fatty liver, hypoglycemia due to insulin hypersecretion, arteriosclerosis, hyperlipidemia, cerebral stroke, obesity, various diseases induced by diabetes or obesity, and the like. | 2010-09-09 |
| 20100227351 | METHOD OF DETECTING AND SEPARATING SERUM GAMMA-GLUTAMYL TRANSFERASE (GGT) ISOFORMS IN A SAMPLE OF BIOLOGICAL FLUID AND THE ENZYME ISOFORMS THEREBY OBTAINED - A method is described for detecting the gamma-glutamyl transferase enzyme isoforms (GGT, EC 2.3.2.2) in a sample of biological fluid, such as for example plasma or serum. The method comprises an HPLC separation step of the sample proteins based on the molecular size and a second step for detecting the GGT isoforms by post-column reaction with a GGT enzyme substrate capable of generating a detectable final product, preferably by spectrophotometric or fluorimetric means. The GGT isoforms can be separated by ultra-centrifugation, thereby obtaining three enzymatic isoforms characterised by molecular weights of approximately 2000, 940, and 140 KDa, respectively. | 2010-09-09 |
| 20100227352 | Expression Quantification Using Mass Spectrometry - In various aspects, the present teachings provide systems, methods, assays and kits for the absolute quantitation of protein expression. In various aspects, the present teachings provide methods of determining the concentration of one or more proteins of interest in one or more samples of interest. In various aspects, the present teachings provide methods of determining the absolute concentration of one or more isoforms of a to protein using standard samples of signature protein fragments and parent-daughter ion transition monitoring (PDITM). In various embodiments, the absolute concentration of multiple isoforms of a biomolecule in a sample, multiple proteins in a biological process, a combination of multiple samples, or combinations thereof, can be determined in a multiplex fashion using the present teachings. In various aspects, provided are methods of assessing the response of a biological system to a chemical agent. | 2010-09-09 |
| 20100227353 | Rapid Peptidoglycan-Based Assay for Detection of Bacterial Contamination - The invention relates to a colorimetric method for detecting bacterial or fungal pathogens by detecting peptidoglycan or (1-3)-β-D-glucan in a sample. | 2010-09-09 |
| 20100227354 | Autoimmune Conditions and NADPH Oxidase Defects - The invention relates to methods and materials involved in diagnosing and treating autoimmune conditions. In particular, the invention relates to methods and materials involved in diagnosing arthritis conditions that are accompanied by an NADPH oxidase deficiency, methods and materials involved in treating, preventing, or delaying the onset of arthritis conditions that are accompanied by an NADPH oxidase deficiency, and methods and materials involved in identifying agonists and antagonists of NADPH oxidase activity. | 2010-09-09 |
| 20100227355 | REAGENT COMPOSITIONS FOR USE IN ELECTROCHEMICAL DETECTION - Biological reagent compositions with improved sensitivity to the concentration of blood glucose in patient samples for use in measuring systems and methods. The reagent compositions comprise a glucose oxidoreductase enzyme, a flavin nucleoside coenzyme and a mediator formulation. The mediator formulation comprises at least one electroactive organic molecule and at least one coordination complex. | 2010-09-09 |
| 20100227356 | METHODS FOR ASSESSING EFFECTS ON SKELETAL GROWTH - A method for assessing effects on growth of skeletal members comprises preparing a culture comprising a skeletal member and a substance whose effect on growth of the skeletal member is to be determined and capturing images of the skeletal member at selected time intervals without terminating growth. The skeletal member may be fully articulated and the substance whose effect on growth is to be determined may be labeled with a marker and tracked over time. The methods of the invention may be used to assess effects on growth through the positioning of the substance in the skeletal member and for drug discovery. | 2010-09-09 |
| 20100227357 | FUNGAL ISOLATES AND THEIR USE TO CONFER SALINITY AND DROUGHT TOLERANCE IN PLANTS - The present invention is directed to methods and compositions of endophytic fungi that confer stress tolerance to inoculated plants, including both monocots and dicots. In particular, | 2010-09-09 |
| 20100227358 | MINERALIZED THREE-DIMENSIONAL BONE CONSTRUCTS - The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics. | 2010-09-09 |
| 20100227359 | System and method for detection of substances - A system, a method, and apparatus for determining the presence of an agent in a sample. The system includes a sampler for collecting the sample, and an attachment means for attaching the sampler to the sample. In addition, the system includes a carrier, wherein the sampler with the sample is substantially aligned with a portion of the carrier. The system also includes an analyzing device which is substantially aligned with the carrier and the sample. Finally, the system includes a chemistry for identifying an indicator of the agent in the sample as determined by the analyzing device. | 2010-09-09 |
| 20100227360 | SALMONELLA SELECTIVE ENRICHMENT MEDIUM CONTAINING TETRATHIONATE AND MAGNESIUM SALT - The invention relates to a salmonella selective enrichment medium containing tetrathionate or a salt thereof and at least one type of magnesium salt and to a method for detecting salmonella in a sample by using said selective enrichment medium. | 2010-09-09 |
| 20100227361 | HOST CELL MODIFICATIONS THAT IMPROVE PEPTIDE PRODUCTION AND DOWNSTREAM PROCESSING - Disrupting the expression of endogenous | 2010-09-09 |
| 20100227362 | Novel YLMPO1 Gene Derived From Yarrowia Lipolytica and A Process for Preparing a Glycoprotein Not Being Mannosylphosphorylated By Using a Mutated Yarrowia Lipolytica in Which YLMPO1 Gene is Disrupted - The present invention relates to a novel YlMPOl gene which plays an important role in mannosylphosphorylation of an industrial yeast | 2010-09-09 |
| 20100227363 | ALG3 MUTANT - The present invention relates to a method to produce proteins reduced in high mannose type glycans in a plant or plant cell by partially inhibiting the expression of the Alg3 gene in said plant or plant cell. Said partial inhibition can be provided for by inserting the Alg3 mutant gene from | 2010-09-09 |
| 20100227364 | Novel Lipolytic Enzyme ELIP - The present invention provides a novel nucleic acid sequence, designated ELIP, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same. | 2010-09-09 |
| 20100227365 | Methods of using isolated glucose isomerase - This invention provides a series of recombinant | 2010-09-09 |
| 20100227366 | METHOD OF PRODUCING POLYSACCHARIDES FROM COFFEE BEANS OR/AND COFFEE EXTRACTION RESIDUE - A process for efficiently producing a polysaccharide from coffee beans or/and a coffee extraction residue. Use of these has not received attention because of difficulty in decomposing these. The process, which is for producing a polysaccharide from coffee beans or/and a coffee extraction residue, comprises: a step (a) in which the coffee beans or/and coffee extraction residue are reduced to particles having a diameter of 10.0 μm to 5.0 mm; a step (b1) in which after the step (a), the particles are heated at 50-100° C. in the presence of a dilute alkali; a step (c1) in which after completion of the step (b1), cellulase is caused to act thereon; a step (b2) in which after the step (c1), a heat treatment is conducted at 120° C. or higher in the presence of a dilute alkali; and a step (c2) in which after the step (b2), cellulase is caused to act thereon. | 2010-09-09 |
| 20100227367 | Process of Producing a Fermentation Product - The invention relates to a process for producing a fermentation product from molasses wherein molasses is i) treated with a combination of alpha-amylase and glucoamylase and ii) fermented using one or more fermenting organisms at a cell count in the range from 10 | 2010-09-09 |
| 20100227368 | RENEWABLE ENERGY SYSTEM - A renewable energy system and related process includes a biomass unit for receiving biomass energy sources, e.g., municipal wastes, used tires and wood waste. The biomass is fed to a power plant which produces electricity and steam which are fed to several units of the system. A bio-diesel unit uses the electricity to convert vegetable oil and animal fat into a bio-diesel fuel. An algae farm uses the electric power and steam in the form of heat to produce algae oil which is fed to the bio-diesel unit and which also is biomass. A corn unit and a corn stover unit produce ethanol. The corn unit also produces corn oil which is fed to the bio-diesel unit, and residue corn becomes dry distilled grains. A fermentation unit discharges carbon dioxide which along with that discharged by the power plant is distributed to the algae farm for growing algae. Also described is a method for converting carbon dioxide to oxygen through the use of algae. | 2010-09-09 |
| 20100227369 | System for Fermentation of Biomass for the Production of Ethanol - A biorefinery and a system for producing a fermentation product from biomass are disclosed. The biorefinery comprises a preparation system to prepare the biomass into prepared biomass; a pre-treatment system to pre-treat the prepared biomass with a dilute acid for separation into a first component from which pentose can accessed for fermentation and a second component from which hexose can be made available for fermentation; a first treatment system to treat the first component into a treated first component by removing removed components from the first component; a first fermentation system to produce a first fermentation product from the pentose; a distillation system to recover ethanol from the first fermentation product; and a treatment system to process removed components. The biomass comprises lignocellulosic material, which comprises at least one of corn cobs, corn plant husks, corn plant leaves and corn plant stalks. | 2010-09-09 |
| 20100227370 | METHOD OF PRODUCING LYCOPENE USING RECOMBINANT ESHERICHIA COLI - A method of producing lycopene, with high productivity by means of a recombinant bacterial strain includes preparing the recombinant vector containing genes encoding proteins, which are required for lycopene biosynthesis. The genes involved in lycopene biosynthesis are crtE, crtB and crtI, and at least one of the said three genes (crtE, crtB and crtI) is selected from the group consisting of crtE with the nucleotide Sequence 1, crtB with the nucleotide Sequence 3 and crtI with the nucleotide Sequence 5, of the Sequence List. The said recombinant vector is transformed into | 2010-09-09 |
| 20100227371 | Electrosonic Cell Manipulation Device - In method of injecting a substance into a living cell having a cell membrane, the substance, the cell and a liquid are placed into a tapering passage. Energy is applied to the cell, thereby inducing poration. To sort cells, a cellular suspension is placed in a tapering passage, including a narrow end that defines an opening that has a dimension corresponding to a cell size. An acoustic wave is applied, thereby forcing cells having a cell size smaller than the selected cell size through the opening, with a portion of the cells having a cell size not smaller than the selected cell size not forced through the opening. To extract material from a cell, an electric field and an acoustic wave are applied, thereby causing the cell membrane to allow the material to pass out of the cell. | 2010-09-09 |
| 20100227372 | BIOLOGICAL FUNCTIONALISATION OF SUBSTRATES - The invention relates to an activated metallic, semiconductor, polymer, composite and/or ceramic substrate, the substrate being bound through a mixed or graded interface to a hydrophilic polymer surface that is activated to enable direct covalent binding to a functional biological molecule, the polymer surface comprising a sub-surface that includes a plurality of cross-linked regions, as well as to such activated substrates that have been functionalised with a biological molecule and to devices comprising such functionalised substrates. Such substrates can be produced by a method comprising steps of: a. exposing a surface of the substrate to any or more of (i) to (iii): (i) plasma ion implantation with carbon containing species; (ii) co-deposition under conditions in which substrate material is deposited with carbon containing species while gradually reducing substrate material proportion and increasing carbon containing species proportion; (iii) deposition of a plasma polymer surface layer with energetic ion bombardment; incubating the surface treated according to step (a) with a desired biological molecule. | 2010-09-09 |
| 20100227373 | Recombinant Carrier Molecule for Expression, Delivery and Purification of Target Polypeptides - Recombinant carrier molecules having amino acid sequences from thermostable enzymes and methods of use for expression, recovery and delivery of foreign sequences (peptides and polypeptides) produced in different systems (bacteria, yeast, DNA, cell cultures such as mammalian, plant, insect cell cultures, protoplast and whole plants in vitro or in vivo are provided. The recombinant carrier molecule using sequences from lichenase B(Lic B) were also made and used as part of carrier protein to express, recover and deliver a variety of target polypeptides of interest. | 2010-09-09 |
| 20100227374 | PROTEIN STABILIZATION BY DOMAIN INSERTION INTO A THERMOPHILIC PROTEIN - A strategy to improve protein stability by domain insertion. TEM 1 beta-lactamase (BLA) and exo-inulinase, as model target enzymes, are inserted into a hyperthermophilic maltose binding protein from | 2010-09-09 |
| 20100227375 | Polypeptides Having Lipase Activity and Polynucleotides Encoding Same - The present invention relates to polypeptide having lipase activity and which further has a RP of at least 0.8 and a BR of at least 1.1 at the test conditions given in the specification. | 2010-09-09 |
| 20100227376 | METHOD OF OBTAINING BACTERIOPHAGE PREPARATION - New method of removing pyrogens from bacteriophage preparations based on the natural processes of endotoxin degradation, and consisting of several stages, including in particular the addition of metal ions, the heterophase extraction, and chromatography. | 2010-09-09 |
| 20100227377 | Method for sustaining Microorganism culture in Syngas fermentation process in decreased concentration or absence of various substrates - The present invention relates to methods for sustaining microorganism culture in a syngas fermentation reactor in decreased concentration or absence of various substrates comprising: adding carbon dioxide and optionally alcohol; maintaining free acetic acid concentrations; and performing the above mentioned steps within specified time. | 2010-09-09 |
| 20100227378 | Centrifugation and Filtration Methods for Concentrating Microorganisms - A process for concentrating microorganism-containing suspensions comprising (a) centrifuging a microorganism-containing suspension to provide a first microorganism-containing concentrate and a supernatant liquid; (b) filtering said first microorganism-containing concentrate, to provide a permeate and a second microorganism-containing concentrate. | 2010-09-09 |
| 20100227379 | Compact disk based system for separating immunomagnetic bead labeledparticulates and method thereof - Disclosed is a disk based system for separating at least two types of particulates contained in a sample fluid. The system includes a disk-like carrier board and a magnetic attraction unit. The disk-like carrier board forms at least one flow channel structure, which includes an inner reservoir, at least one separation chamber, and at least one outer reservoir arranged in sequence from a geometric center of the disk-like carrier board to an outer circumferential rim of the disk-like carrier board. A method of separation carried out with the system includes introducing the sample fluid into the inner reservoir and then rotating the disk-like carrier board to induce a centrifugal force. The sample fluid contains particulates that are labeled with immunomagnetic beads and the labeled particulates are attracted by the magnetic force generated by the magnetic attraction unit to retain in the inner reservoir or the separation chamber. Particulates not labeled with the immunomagnetic beads and contained in the sample fluid move with the sample fluid to the outer reservoir. | 2010-09-09 |
| 20100227380 | BIOREMEDIATION - The chemical oxygen demand of unprocessed, spent metal working fluids, can be reduced to levels of 2000 mg l | 2010-09-09 |
| 20100227381 | ENHANCED BIODEGRADATION OF NON-AQUEOUS PHASE LIQUIDS USING SURFACTANT ENHANCED IN-SITU CHEMICAL OXIDATION - A method for in-situ reduction of contaminants in soil that uses chemical oxidation and biodegradation. | 2010-09-09 |
| 20100227382 | Nanoscale sensors - Various aspects of the present invention generally relate to nanoscale wire devices and methods for use in determining analytes suspected to be present in a sample, and systems and methods of immobilizing entities such as reaction entities relative to nanoscale wires. In one aspect, a nucleic acid, such as DNA, may be immobilized relative to a nanoscale wire, and in some cases, grown from the nanoscale wire. In certain embodiments, the nucleic acid may interact with entities such as other nucleic acids, proteins, etc., and in some cases, such interactions may be reversible. As an example, an enzyme such as telomerase may be allowed to bind to DNA immobilized relative to a nanoscale wire. The telomerase may extend the length of the DNA, for instance, by reaction with free deoxynucleotide triphosphates in solution; additionally, various properties of the nucleic acid may be determined, for example, using electric field interactions between the nucleic acid and the nanoscale wire. In another aspect, the invention provides systems and methods for attaching entities such as nucleic acids, receptors such as gangliosides, or surfactants to a nanoscale wire, for example, using aldehyde-producing reactions or hydrophobic interactions. In some aspects, certain systems and methods of the present invention may be used to determine an analyte suspected to be present in a sample, for example, a toxin, a virus, or a small molecule. Systems and methods of using such nanoscale wires are disclosed in other aspects of the invention, for example, within a microarray. Still other aspects of the invention include assays, sensors, kits, and/or other devices that include such nanoscale wires, methods of making and/or using functionalized nanoscale wires (for example, in drug screening or high-throughput screening), and the like. | 2010-09-09 |
| 20100227383 | GENE DETECTION AND DETERMINATION APPARATUS, GENE REACTOR, AND INCUBATOR - A genetic detection and determination apparatus which detects or determines genetic information, provided with a reaction container having a reaction vessel, including: a container set portion on which the reaction container is set; a moving mechanism which moves the container set portion along a track; a reaction vessel dividing portion which divides the reaction vessel into a plurality of reaction chambers by deforming the reaction vessel; a heating and cooling portion which heats and cools the reaction container; and a measuring portion which is movable above the container set portion in parallel to an upper surface of the reaction container provided on the container set portion and which measures a reaction within each of the reaction chambers. | 2010-09-09 |
| 20100227384 | Portable Genomic Analyzer - An apparatus for identifying a target portion of a sample. In some embodiments, the sample can be substantially purified and/or amplified in a test chamber. In some embodiments, the sample can be provided in an amount so that amplification is not necessary. The apparatus generally comprises a test chamber having an input region for receiving a sample and an analysis region. A sample chamber can be defined by at least a portion of the test chamber. A separation mechanism can separate a target portion of the sample from the sample and an analysis chamber can aid in analyzing at least the target portion of the sample. | 2010-09-09 |
| 20100227385 | METHOD AND DEVICE FOR MEASURING MULTIPLE PHYSIOLOGICAL PROPERTIES OF CELLS - A method of analyzing cells disposed in media within a vessel includes the steps of providing a vessel having an original volume of media about the cells, reducing the original volume of media about at least a portion of the cells to define a reduced volume of media, and analyzing a constituent related to the cells within the reduced volume of media. An apparatus for analyzing cells includes a stage adapted to receive a vessel holding cells and a volume of media, a plunger adapted to receive a barrier to create a reduced volume of media within the vessel including at least a portion of the cells, the barrier adapted for insertion into the vessel by relative movement of the stage and the plunger, and a sensor in sensing communication with the reduced volume of media, wherein the sensor is configured to analyze a constituent disposed within the reduced volume. | 2010-09-09 |
| 20100227386 | COMPACT OPTICAL DETECTION SYSTEM - A detection system is provided, the detection system comprising a light source that generates excitation light having a wavelength sufficient to excite a fluorophore in a sample; an excitation filter positioned along a first line along a path of the excitation light, the excitation filter transmitting the excitation light from the light source; a beam splitter positioned along the first line, the beam splitter reflecting the excitation light transmitted by the excitation filter along a second line toward a mirror positioned on one side of the beam splitter, and passing emitted light reflected along the second line; the mirror, positioned to reflect the excitation light from the beam splitter to the fluorophore in the sample along a third line, normal to both the first and second lines, wherein the mirror further reflects emitted light emitted along the third line, along the second line toward the beam splitter; an emission filter positioned along the second line, on a second side of the beam splitter; and a detector that detects the emitted light transmitted by the emission filter. | 2010-09-09 |
| 20100227387 | Structure and method for handling magnetic particles in biological assays - Magnetic handling structure ( | 2010-09-09 |
| 20100227388 | Reactor for growing cultures - A reactor for growing a culture has a plurality of sections located above each other in a vertical direction and each having a peripheral surface, a bottom, and a top forming together a container for accommodating a solution for growing a culture, and a plurality of light sources arranged on the top so that they emit light into an interior of the container. | 2010-09-09 |
| 20100227389 | Biological reactor - A biological reactor for growing a biological mass has a tank with an interior for growing the biological mass, a plate movable in a substantially vertical direction and an interior of the tank, and a valve means provided with the plate and configured so that in a lower position of the plate the valve means are open, so that during displacement of the plate upwardly and downwardly, a solution in the interior of the tank is thoroughly fixed, while an upper position of the plate, the valve means are closed and the plate during movement downwardly provides a collection of a concentrated solution to be removed from the tank. | 2010-09-09 |
| 20100227390 | Ethanol Water Solution Concentrating Device - A device for concentrating ethanol from an ethanol water solution yielded from lignocellulose is equipped with a water separation membrane, an ethanol water solution storing unit and a depressurizing container provided so as to sandwich the water separation membrane, a dry gas cylinder for supplying dry gas to an ethanol vapor residing unit above the ethanol water solution storing unit, a pump for sucking in the interior of the ethanol vapor residing unit, and a cold trap for collecting the concentrated ethanol by condensing the ethanol vapor sucked in by the pump. | 2010-09-09 |
| 20100227391 | Device for squeezing of biological mass of cultures - A device for wringing a biological mass of cultures has a substantially vertical container having a bottom provided with a discharge opening, a rotor means located under the discharge opening and including at least two rotors which are rotatable toward one another to receive a biological mass therebetween, wherein the rotors are provided with a plurality of substantially radially extending plungers which are displaceable in a radial direction. | 2010-09-09 |
| 20100227392 | Use of RPA32, RPA4, and RPA4/RPA32 Hybrid Polypeptides and Polynucleotides for Assessing and Modulating Cell Proliferation - Disclosed are polynucleotides and polypeptides that are useful for assessing and modulating cell proliferation. The polynucleotides may comprise coding regions for RPA4 and RPA4/RPA32 hybrid polypeptides. The polynucleotides may be used in gene vectors for modulating cell proliferation in a patient in need thereof. Diagnostic methods related to assaying RPA4 expression in test samples in order to detect proliferating or cancerous cells also are disclosed. | 2010-09-09 |
| 20100227393 | LIVER STEM CELLS: ISOLATION OF HEPATIC PROGENITOR CELLS FROM THE HUMAN GALL BLADDER - Methods of preparing multipotent cell populations from gallbladder are provided. Also provided are enriched multipotent cell populations from gallbladder and methods of differentiating the multipotent cell populations to prepare, for example, hepatocytes. | 2010-09-09 |
| 20100227394 | FUSION ANTIBODIES - The present invention provides novel antibodies. In particular, the present invention provides fusion antibodies comprising antibody heavy and light chain fusions. The present invention further provides multivalent antibodies comprising multiple fusion antibody chains. The present invention further provides methods of generating splice resistant antibody genes. | 2010-09-09 |
| 20100227395 | CANINE TUMOR CELL AND ALLOGENEIC DENDRITIC CELL FUSED VACCINE AND METHOD FOR PREPARING THE SAME - The present invention provides a dendritic cell-based vaccine by fusing a canine tumor cell and an allogeneic dendritic cell, and a method for preparing the same. The fusion cells expressing canine tumor antigens are generated by fusing canine bone marrow-derived dendritic cells and canine tumor cells. The canine immune system can be induced to produce tumor specific T lymphocytes and natural killer cells when the fusion cells used as a vaccine is injected into a canine body. | 2010-09-09 |
| 20100227396 | Embryonic Stem Cell-Like Cells - Disclosed is a method for preparing an embryonic stem cell (ESC)-like cell, which includes the steps of: (a) obtaining a first cell population from a mammalian tissue or body fluid, wherein the first cell population comprises adult stem cells; (b) obtaining a second somatic cell population from a mammalian tissue, wherein the mammalian tissue is different from the mammalian tissue in step (a) and the second cell population is different from the first cell population; (c) coculturing the first cell population and the second cell population in a medium for a period of time sufficient to form a colony from either the first cell population or the second cell population; and (d) subculturing a cell from the colony in a medium for a period time sufficient to prepare the ESC-like cell. | 2010-09-09 |
| 20100227397 | Preparation method of a hair dermal papilla cell preparation, composition and method for regenerating hair follicles, and animal having regenerated hair follicles - The present invention provides a method for preparing a hair dermal papilla cell preparation comprising preparing a cell suspension by removing epidermal tissue from skin tissue and subjecting the resulting dermal tissue fraction to collagenase treatment, and cyropreserving the cell suspension to kill the follicular epidermal cells. The present invention also provides a composition for regenerating hair follicles comprising hair dermal papilla cell and epidermal cells, wherein the ratio of the number of hair dermal papilla cell to the number of epidermal cells is from 1:10 to 10:1. | 2010-09-09 |
| 20100227398 | Method for Mixing Object into Gelled Assembly - This invention provides a mixing method by which a mixing object can be uniformly mixed into a gelled assembly within a short time period. The method for mixing a mixing object into a gelled assembly comprises freezing the gelled assembly; melting the frozen assembly to obtain a sol; mixing the resultant sol and the mixing object; and reconstituting the gelled assembly from the sol into which the mixing object has been mixed. | 2010-09-09 |
| 20100227399 | SOFT GEL SYSTEMS IN MODULATING STEM CELL DEVELOPMENT - This invention provides gels and matrices having a rigidity in the range of 150-750 Pa, methods of manufacturing same, and method of preserving a mesenchymal stem cell population or studying mesenchymal stem cells, comprising same. | 2010-09-09 |
| 20100227400 | MONOMERIC AND DIMERIC FLUORESCENT PROTEIN VARIANTS AND METHODS FOR MAKING SAME - The present invention relates generally to fluorescent proteins and fluorescent protein variants, and more specifically to monomeric and dimeric forms of Anthozoan fluorescent proteins. In one aspect, the present invention provides variants of fluorescent proteins, where the variants have a reduced propensity to tetramerize, and form dimeric or monomeric structures. In a further aspect, the present invention provides variants of fluorescent proteins, the variants being characterized by more efficient maturation than corresponding fluorescent proteins from which they are derived. The invention also relates to methods of making and using such fluorescent proteins and fluorescent protein variants, including fluorescent protein monomers and dimers. | 2010-09-09 |
| 20100227401 | MEDIA AND PROCESSES FOR THE EX VIVO PRODUCTION OF MEGAKARYOCYTES FROM HUMAN CD34+ CELLS - Disclosed herein are media and processes for the ex vivo production of megakaryocytes from human CD34 | 2010-09-09 |
| 20100227402 | Cell Surface Coating with Hyaluronic Acid Oligomer Derivative - A method of localising reproduction assisting hyaluronic acid to reproductive cells surfaces by covalently linking it to lipids is disclosed. | 2010-09-09 |
| 20100227403 | rbLIF PROTEIN FOR USE IN EMBRYONIC STEM CELL CULTURES - Provided herein methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell on a rabbit feeder cell layer, wherein the rabbit feeder layer expresses a leukemia inhibitory factor (LIF) protein. Also provided are methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell in a culture medium comprising rabbit leukemia inhibitory factor (rbLIF) protein. Also provided are polynucleotide and amino acid sequences of a rabbit leukemia inhibitory factor protein (rbLIF). | 2010-09-09 |
| 20100227404 | WATER EXTRACT OF ANTRODIA CAMPHORATA FOR IMMUNOSTIMULATORY EFFECT AND PREPARATION METHOD THEREOF - A preparation method for a water extract of the fruiting body of | 2010-09-09 |
| 20100227405 | METHODS OF USING MULTILAYERED CELL CULTURE APPARATUS - A multilayered cell culture apparatus for the culturing of cells is disclosed. The cell culture apparatus is defined as an integral structure having a plurality of cell culture chambers in combination with tracheal space(s). The body of the apparatus has imparted therein gas permeable membranes in combination with tracheal spaces that will allow the free flow of gases between the cell culture chambers and the external environment. The flask body also includes an aperture that will allow access to the cell growth chambers by means of a needle or cannula. The size of the apparatus, and location of an optional neck and cap section, allows for its manipulation by standard automated assay equipment, further making the apparatus ideal for high throughput applications. | 2010-09-09 |
| 20100227406 | METHOD FOR PURIFYING CELLS, RECOVERING CELLS, AND TRANSFECTING CELLS GENTLY - The present invention relates to a process for cell purification, for cell recovery from cultures and for the transfection of the cells under especially gentle conditions, where this process can additionally be coupled with subsequent isolation and purification of polynucleotides from the optionally transfected cells, and a kit and a device for the implementation of this process. | 2010-09-09 |
| 20100227407 | AAV4 VECTOR AND USES THEREOF - The present invention provides an adeno-associated virus 4 (AAV4) virus and vectors and particles derived therefrom. In addition, the present invention provides methods of delivering a nucleic acid to a cell using the AAV4 vectors and particles. | 2010-09-09 |
| 20100227408 | Method and apparatus for avalanche-mediated transfer of agents into cells - The present invention provides a method and apparatus for transferring an agent into a cell. The method includes the steps of providing an agent outside of a cell and generating a vapor bubble and a plasma discharge between an avalanche electrode and a conductive fluid surrounding the cell. The vapor bubble and plasma discharge generate a mechanical stress wave and an electric field, respectively. The combination of this mechanical stress wave and electric field results in permeabilization of the cell, which in turn results in transfer of the agent into the cell. | 2010-09-09 |
