| 36th week of 2008 patent applcation highlights part 43 |
| Patent application number | Title | Published |
|---|
| 20080213818 | CELLULAR IMMUNITY TEST WITH PEPTIDES ATTACHED TO A SOLID SUPPORT - The invention concerns a method for detecting cellular immunity (with respect to an antigen), using a solid support whereon is fixed an assortment of peptides constituting T cell epitopes of the antigen to be tested, kits for implementing said method. | 2008-09-04 |
| 20080213819 | Device for Preparing a Body Fluid for a Bacteriological Analysis - The invention concerns a device for preparing a body fluid for a bacteriological analysis thereof comprising a container provided with a chamber ( | 2008-09-04 |
| 20080213820 | Method for Determining the Selectivity of a Pre-Synaptic Neuromuscular Blocking Substance - The invention relates to a method for determining the quantity of pre-synaptic neuromuscular blocking substance (notably botulinum toxin) contained in a sample. In one aspects, the method comprises the following steps:
| 2008-09-04 |
| 20080213821 | Microfluidic Cell Sorter System - A microfluidic system for separating, purifying and counting cell sub-populations, utilising steering of liquid flows in microfluidic channels in a cell focusing region (first dotted circle area); having the integration of the optical detection mechanism and a microchannel structure made from moulding. A master is photolithographically patterned on a soft PDMS silicon or polymer material. After being moulded and peeled off the master, the micro-channel structure is sealed on a hard substrate with openings punched through for wells ( | 2008-09-04 |
| 20080213822 | METHODS FOR DIAGNOSING CELIAC SPRUE AND REAGENTS USEFUL THEREIN - Administering an effective dose of glutenase to a Celiac or dermatitis herpetiformis patient reduces levels of toxic gluten oligopeptides, thereby attenuating or eliminating the damaging effects of gluten. | 2008-09-04 |
| 20080213823 | CAPILLARY-CHANNELED POLYMER FILM FLOW CYTOMETRY - The present invention relates to methods for counting, sorting, and manipulation of cells, organelles, and/or cellular material using capillary-channeled polymer films. | 2008-09-04 |
| 20080213824 | Synthesis of Labeled Probes - Improvements in FISH staining employing an aqueous acetonitrile/dimethylsulfoxide mixture in a fluorophore coupling reaction. | 2008-09-04 |
| 20080213825 | Hyperbaric Criogenesis Chambers - The Hyperbaric Cryogenesis Chambers are equipment for medical usage, extracorporeal, and capable of promoting the proliferation and preservation of cells in a way mainly microbiologic, “not biologic”. They are compartments with containers that host tissues or cells in solution with nutrients, that bear pressures higher and lower to sea level They have a cold system inside that lowers the environmental temperature and maintains it permanently. They are for maintaining viability of cells or tissues and to induce their organized proliferation with oxygen or other gases at pressures higher or lower that the exterior of the chamber. | 2008-09-04 |
| 20080213826 | Compound microbial preparation manufacturing process - A manufacturing process for making compound microbial preparation to improve soil quality, activate soil, effectively degrade soil pollution, and help growth of crops is comprised of having first individual cultivation of multiple single microbial series; each microbial series during the individual cultivation producing multiple life organisms; followed by crossing cultivation among those single microbial series in a specific sequence to develop a compound microbial preparation containing multiple single microbial series, multiple hormones produced by individual microbial series, and hormones produced by each single microbial series during the cross cultivation. | 2008-09-04 |
| 20080213827 | Process For Producing Dipeptides or Dipeptide Derivatives - The present invention provides a process for producing a dipeptide or a dipeptide derivative by using a protein having the activity to form the dipeptide or dipeptide derivative from one or more kinds of amino acids or amino acid derivatives, or a culture of cells having the ability to produce the protein or a treated matter of the culture as a enzyme source, which comprise; | 2008-09-04 |
| 20080213828 | Glycoprotein synthesis - Methods for making glycoproteins, both in vitro and in vivo, are provided. One method involves incorporating an unnatural amino acid into a protein and attaching one or more saccharide moieties to the unnatural amino acid. Another method involves incorporating an unnatural amino acid that includes a saccharide moiety into a protein. Proteins made by both methods can be further modified with additional sugars. | 2008-09-04 |
| 20080213829 | Materials and methods to increase peptide chain expression - DNA sequences that increase peptide chain expression when operably linked to a gene encoding the peptide chain and methods of generating a peptide chain expression host cell using the foregoing are disclosed. Peptide chain expression host cells are also disclosed. | 2008-09-04 |
| 20080213830 | Degradable Clostridial Toxins - The specification discloses modified Clostridial toxins comprising a PAR ligand domain, a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and a Clostridial toxin binding domain; polynucleotide molecules encoding modified Clostridial toxins comprising a PAR ligand domain, a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and a Clostridial toxin binding domain; and method of producing modified Clostridial toxins comprising a PAR ligand domain, a Clostridial toxin enzymatic domain, a Clostridial toxin translocation domain and a Clostridial toxin binding domain. | 2008-09-04 |
| 20080213831 | HUMAN CYTOKINE RECEPTOR - Cytokines and their receptors have proven usefulness in both basic research and as therapeutics. The present invention provides a new human cytokine receptor designated as “Zcytor18.” | 2008-09-04 |
| 20080213832 | SESQUITERPENE SYNTHASES AND METHODS OF USE - The invention relates to sesquiterpene synthases and methods of their production and use. In one embodiment, the invention provides nucleic acids comprising a nucleotide sequence as described herein that encodes for at least one sesquiterpene synthases. In a further embodiment, the invention also provides for sesquiterpene synthases and methods of making and using these enzymes. For example, sesquiterpene synthases of the invention may be used to convert farnesyl-pyrophosphate to various oxygenated and aliphatic sesquiterpenes including valencene, bicyclo-germacrene, cubebol and delta-cadinene. | 2008-09-04 |
| 20080213833 | Methods for Obtaining Optically Active Glycidyl Ethers and Optically Active Vicinal Diols from Racemic Substrates - The invention provides yeast strains, and polypeptides encoded by genes of such yeast strains, that have enantiospecific glycidyl ether hydrolase activity. The invention also features nucleic acid molecules encoding such polypeptides, vectors containing such nucleic acid molecules, and cells containing such vectors. Also embraced by the invention are methods for obtaining optically active glycidyl ethers and associated optically active vicinal diols. | 2008-09-04 |
| 20080213834 | GSK3 LIGANDS AND POLYNUCLEOTIDES ENCODING GSK3 LIGANDS - The invention relates to kinase ligands and polyligands. In particular, the invention relates to ligands and polyligands that modulate GSK3 activity. The ligands and polyligands are utilized as research tools or as therapeutics. The invention includes linkage of the ligands and polyligands to a cellular localization signal, epitope tag and/or a reporter. The invention also includes polynucleotides encoding the ligands and polyligands. | 2008-09-04 |
| 20080213835 | Polypeptides Having Cellobiohydrolase II Activity And Polynucleotides Encoding Same - The present invention relates to polypeptides having cellobiohydrolase II activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. | 2008-09-04 |
| 20080213836 | Modified enteropeptidase protein - Disclosed are novel enteropeptidase polypeptides, polynucleotides encoding the polypeptides, nucleotide constructs, vectors, host cells comprising the polynucleotides, and methods for producing the polypeptides and polynucleotides. Such polypeptides are useful as protein engineering tool for enzymatic cleavage of fusion proteins. Also provided are kits comprising the polypeptides of the invention. | 2008-09-04 |
| 20080213837 | Apo-3 ligand polypeptide - A tumor necrosis factor and lymphotoxin homolog having apoptotic activity, identified as Apo-3 Ligand, is provided. Nucleic acid molecules encoding Apo-3 Ligand, chimeric molecules and antibodies to Apo-3 Ligand are also provided. | 2008-09-04 |
| 20080213838 | DNA, vector, transformant and method for producing APA protein - It is intended to provide a production method by which human APA protein can be recovered more efficiently and in a larger quantity compared to a conventional method; DNA encoding a recombinant human APA protein which is necessary when the human APA protein is produced; a vector in which the DNA is integrated; and a transformant which retains the vector. | 2008-09-04 |
| 20080213839 | Methods and materials relating to stem cell growth factor-like polypeptides and polynucleotides - The invention provides novel polynucleotides and polypeptides encoded by such polynucleotides and mutants or variants thereof that correspond to a novel human secreted stem cell growth factor-like polypeptides. Other aspects of the invention include vectors containing messes for producing novel human secreted stem cell growth factor-like polypeptides, and antibodies specific for such polypeptides. | 2008-09-04 |
| 20080213840 | Compositions Containing, Methods Involving, and Uses of Non-Natural Amino Acids and Polypeptides - Disclosed herein are non-natural amino acids and polypeptides that include at least one non-natural amino acid, and methods for making such non-natural amino acids and polypeptides. The non-natural amino acids, by themselves or as a part of a polypeptide, can include a wide range of possible functionalities, but typical have at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Also disclosed herein are non-natural amino acid polypeptides that are further modified post-translationally, methods for effecting such modifications, and methods for purifying such polypeptides. Typically, the modified non-natural amino acid polypeptides include at least one oxime, carbonyl, dicarbonyl, and/or hydroxylamine group. Further disclosed are methods for using such non-natural amino acid polypeptides and modified non-natural amino acid polypeptides, including therapeutic, diagnostic, and other biotechnology uses. | 2008-09-04 |
| 20080213841 | Novel Method for Assembling DNA Metasegments to use as Substrates for Homologous Recombination in a Cell - The invention relates to a novel method for obtaining DNA metasegment comprising ligating adjoining DNA fragments at least 10 Kb in size containing at least one overlapping region. | 2008-09-04 |
| 20080213842 | KIT FOR SYNTHESIZING POLYNUCLEOTIDES - The present invention realized isothermal and rapid polynucleotide synthesis by using as templates polynucleotides having a structure capable of forming loops, and combining a plurality of primers capable of providing a starting point for complementary strand synthesis to such loops. If the LAMP method is applied, all reactions can be carried out isothermally and rapidly since the template polynucleotides themselves can also be synthesized by an isothermal reaction. | 2008-09-04 |
| 20080213843 | Glucoamylase Variants - The invention relates to a variant of a parent fungal glucoamylase, which exhibits altered properties, in particular improved thermal stability and/or increased specific activity. | 2008-09-04 |
| 20080213844 | DURA SUBSTITUTE AND A PROCESS FOR PRODUCING THE SAME - The invention relates to dura substitutes to be used as prostheses for dural defects in the field of neurosurgery and processes for producing the same. The present invention provides artificial dura mater materials comprising sheets of microbial-derived polysaccharide processed to have the necessary strength characteristics, conformability and physical properties. | 2008-09-04 |
| 20080213845 | Method to increase the yield and improve purification of products from transaminase reactions - A process for producing high yields of enantioselective amino acids and chiral amines by reacting a keto acid or ketone and an amino acid donor in the presence of a transaminase biocatalyst to produce a keto acid by-product and an amino acid or amine product. Further reacting the keto acid by-product with a peroxide to increase the yield of additional amino acid or amine product. | 2008-09-04 |
| 20080213846 | Method of production of para-hydroxycinnamic acid using a thermostable TAL enzyme - A thermostable TAL enzyme was identified from the fungus | 2008-09-04 |
| 20080213847 | PROCESS FOR PRODUCING PRENYL ALCOHOLS - A method of producing a prenyl alcohol(s) by culturing a mutant cell into which a fusion gene of farnesyl diphosphate synthase gene and geranylgeranyl diphosphate synthase gene has been introduced and recovering the prenyl alcohol(s) from the resultant culture. | 2008-09-04 |
| 20080213848 | METHODS FOR INCREASING THE PRODUCTION OF ETHANOL FROM MICROBIAL FERMENTATION - A stable continuous method for producing ethanol from the anaerobic bacterial fermentation of a gaseous substrate containing at least one reducing gas involves culturing in a fermentation bioreactor anaerobic, acetogenic bacteria in a liquid nutrient medium; supplying the gaseous substrate to the bioreactor; and manipulating the bacteria in the bioreactor by reducing the redox potential, or increasing the NAD(P)H TO NAD(P) ratio, in the fermentation broth after the bacteria achieves a steady state and stable cell concentration in the bioreactor. The free acetic acid concentration in the bioreactor is maintained at less than 5 g/L free acid. This method allows ethanol to be produced in the fermentation broth in the bioreactor at a productivity of greater than 10 g/L per day. Both ethanol and acetate are produced in a ratio of ethanol to acetate ranging from 1:1 to 20:1. | 2008-09-04 |
| 20080213849 | Ethanol production from solid citrus processing waste - Method for producing ethanol from solid citrus waste by reducing the concentration of limonene in citrus waste to allow fermentation. In one embodiment ground solid citrus waste is partially hydrolyzed and pasteurized by heating using a jet cooker and then injected into a flash tank to remove limonene. The heated citrus waste is then cooled, hydrolyzed with enzymes and fermented to ethanol. The remaining solids and liquids may be processed further to yield other byproducts. More particularly, the solids may be dried and pressed for use in cattle feed and the liquids may be further fermented or processed to yield additional ethanol, acetate, galacturonic acid monomers and polymers, five carbon sugars and other products. | 2008-09-04 |
| 20080213850 | Pretreatment of Waste Mushroom Bed and Method of Converting the Same to Yield Sugars and Ethanol - It is an objective of the present invention to develop a method of pretreating a waste mushroom bed so as to easily and efficiently obtain sugars and ethanol with the use of such waste mushroom bed. It is another objective of the present invention to develop a method of converting the pretreated waste mushroom bed to yield sugars and ethanol. According to the present invention, it has been found that the above objective can be achieved by maintaining a waste mushroom bed at 4° C. to 30° C. for 1 week or longer after harvesting of fruit bodies for conversion of the waste mushroom bed to yield sugars and ethanol. | 2008-09-04 |
| 20080213851 | Enzymatic Method for Producing Bioactive, OsteoblastStimulating Surfaces and Use Thereof - The invention relates to a method for producing bioactive surfaces by enzymatic modification of molecules or molecular aggregates, in particular, collagen, on surfaces of glass, metals, metallic oxides, plastics, biopolymers or other materials with an amorphous silicon dioxide (silica) or silicones in the cell culture, by tissue engineering or in medical implants, whereby a polypeptide is used for enzymatic modification, which contains a silicatein α or silicatein β domain. The inventive method promotes the growth, activity and/or mineralization of cells/cell cultures. | 2008-09-04 |
| 20080213852 | Novel Gene Sts 18 - The present invention relates to newly identified genes that encode proteins that are involved in the synthesis of L-ascorbic acid (hereinafter also referred to as Vitamin C). The invention also features polynucleotides comprising the full-length polynucleotide sequences of the novel genes and fragments thereof, the novel polypeptides encoded by the polynucleotides and fragments thereof, as well as their functional equivalents. The present invention also relates to the use of said polynucleotides and polypeptides as biotechnological tools in the production of Vitamin C from microorganisms, whereby a modification of said polynucleotides and/or encoded polypeptides has a direct or indirect impact on yield, production, and/or efficiency of production of the fermentation product in said microorganism. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the direct production of Vitamin C. | 2008-09-04 |
| 20080213853 | Magnetofluidics - Magnetofluidic systems and techniques. In one aspect, a magnetofluidic device includes a superhydrophobic surface and a fluid sample in physical contact with the superhydrophobic surface, the fluid sample comprising a collection of particles coated with a passivating layer. The particles are magnetically active in that they respond to an applied magnetic field. | 2008-09-04 |
| 20080213854 | DEVICE AND METHOD FOR STABILISING THE FLOW THROUGH A CHAMBER | 2008-09-04 |
| 20080213855 | Planar electroporation apparatus and method - An electroporation apparatus provides for the electroporation of adherent cells attached to an electrode surface or suspended cells in close proximity to an electrode surface. In one embodiment, the electrodes are transparent to allow cell viewing using a microscope or an automated image analysis machine. The geometry of the electrodes and associated electrically non-conductive structures may provide for well-defined regions of electroporated and non-electroporated adherent cells with a clearly defined interface between these regions, facilitating comparison of electroporated cells and non-electroporated cells, and evaluation of transfer of material from cell to cell via intercellular gap junctions. | 2008-09-04 |
| 20080213856 | Method and Device for Isolating Micro-Organisms - A process for isolating at least one micro-organism from a medium including a) introducing a selected quantity of magnetic or magnetizable particles into a sample of the medium; b) incubating the particles and the medium for a time sufficient for the micro-organisms to develop and adhere to surfaces of the particles; c) separating the particles from the medium; d) spreading the particles on a support compatible with development of the micro-organisms; and e) incubating the particles on the support for a time sufficient for the development of colonies corresponding to the isolated micro-organism. | 2008-09-04 |
| 20080213857 | Patterning substrate and cell culture substrate - The present invention intends primarily to provide such as a cell culture patterning substrate that is used to adhere cells in a highly precise pattern on a base material to culture and a cell culture substrate on which cells is adhered in a highly precise pattern. | 2008-09-04 |
| 20080213858 | Stabilization of Enzymes - A process for producing enzymes structures includes providing an emulsion of droplets of a first liquid phase dispersed in a second liquid phase. The one liquid phase is a hydrophilic phase, while the other liquid phase is a hydrophobic phase which is immiscible with the hydrophilic phase. Enzyme molecules are located at or within interfacial boundaries of the droplets and the second liquid phase. The enzyme molecules of the respective droplets are cross-linked so that individual enzyme structures, which are stable and in which the enzymes are immobilized with a majority of active sites of the enzymes being orientated either internally or externally, are formed from individual droplets. | 2008-09-04 |
| 20080213859 | Methods for the Reduction of Stutter in Microsatellite Amplification - The invention provides a method for reducing stutter in the amplification of a microsatellite comprising the steps of providing a sample comprising a microsatellite having a G+C content of 50% or less; contacting the sample with at least one enzyme having nucleic acid polymerase activity; and incubating the sample with the enzyme for a sufficient amount of time and under conditions sufficient to amplify the microsatellite; wherein the incubation is performed in the presence of an amount of betaine, sorbitol or mixtures thereof, effective to reduce stutter relative to the amount of stutter observed in the absence of betaine and/or sorbitol. The invention also provides compositions containing betaine and/or sorbitol, kits for amplifying microsatellites having a G+C content of 50% or less, and methods of using all of the foregoing. | 2008-09-04 |
| 20080213860 | Nicking Endonuclease Methods and Compositions - A nicking endonuclease is described which has an amino acid sequence with at least 70% identity to SEQ ID NO:6 and comprising a mutation at least one of an arginine or gutamic acid corresponding to position 507 and position 546 respectively in SEQ ID NO:6. | 2008-09-04 |
| 20080213861 | Methods and compositions for RNA interference - The present invention provides methods for attenuating gene expression in a cell, especially in a mammalian cell, using gene-targeted double stranded RNA (dsRNA), such as a hairpin RNA. The dsRNA contains a nucleotide sequence that hybridizes under physiologic conditions of the cell to the nucleotide sequence of at least a portion of the gene to be inhibited (the “target” gene). | 2008-09-04 |
| 20080213862 | POLYPEPTIDES HAVING ALPHA-AMYLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to polypeptides having alpha-amylase activity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to a polypeptide having carbohydrate-binding affinity and polynucleotides having a nucleotide sequence which encodes for the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid constructs as well as methods for producing and using the polypeptides. | 2008-09-04 |
| 20080213863 | Method of Controlling Viral Outbreak - Disclosed is a novel application for β-cyclodextrin (β-CD, a cholesterol depletor), and a novel technique for the creation of immunogens. Topical application of β-CD inhibits or reduces the severity of viral outbreaks such as oral or genital herpes by disrupting lipid rafts, through which viral entry and outbreak occur. Viral entry involves virus/lipid raft interaction, wherein the virus unfolds—only in lipid rafts—to enter the cell via the lipid raft. The invention provides a technique for creating immunogens with novel viral epitopes based on the virus/lipid raft interaction and viral unfolding. This fact can be exploited to create novel immunogens based on viral interaction with lipid rafts. The virus/lipid raft co-culture technique creates novel immunogens which will be used to create novel neutralizing monoclonal and polyclonal antibodies to fight viral disease such as HIV infection. | 2008-09-04 |
| 20080213864 | Apparatus For Producing And Handling A Flowing Substance | 2008-09-04 |
| 20080213865 | Compound microbial preparation - A compound microbial preparation includes multiple single microbial series, hormones produced from each of those multiple single microbial series separately cultivated, and hormones produced from cross cultivation among those multiple single microbial series to be applied in modifying soil quality, activating soil, effectively degrading soil pollution, and helping growth of crops. | 2008-09-04 |
| 20080213866 | Method for preparing an expanded product for fermentation - The invention pertains to a method for preparing a product for fermentation comprising the steps:
| 2008-09-04 |
| 20080213867 | Liquid Media for Chlamydospore Production of the Fungus Pochonia Chlamydsporia - This invention refers to the creation of liquid media to grow the fungus | 2008-09-04 |
| 20080213868 | CONCENTRATED AQUEOUS SUSPENSIONS OF MICROALGAE - The invention relates to a method and apparatus for concentrating an aqueous suspension of microalgae. The suspension of microalgae is passed through a tangential filtering device for partially removing water from the suspension without rupturing the microalgae, thereby obtaining a concentrated suspension of microalgae and filtered water. Such a method can be use in systems for production of microalgae. An apparatus for carrying out the method according to the invention is also disclosed. | 2008-09-04 |
| 20080213869 | Hydrogel for separating cell and method of separating cell - Cell/organism is separated by using a hydrogel capable of selectively moving a cell in accordance with a difference in the concentration of a physiologically active substance. The invention provides a hydrogel capable of conducting separation (fractionation, differential separation, fractional collection) of cell/organism having a variety of taxes, which could not be attained in the prior art. | 2008-09-04 |
| 20080213870 | Methods for obtaining modified DNA from a biological specimen - The present invention provides a method for obtaining modified DNA from a biological specimen by obtaining a cell suspension from the specimen, if necessary; passing the cell suspension through a first filter under conditions sufficient to obtain filter-bound cells and suspended DNA; lysing the filter-bound cells under conditions sufficient to release cellular DNA; modifying the DNA bound to the filter under conditions sufficient to release the modified DNA from the filter into a flow-through volume; passing the flow-through volume through a second filter under conditions sufficient to capture the modified DNA to the second filter; and eluting the modified DNA from the second filter. | 2008-09-04 |
| 20080213871 | Altering regulation of maize lignin biosynthesis enzymes via RNAi technology - The present invention relates to compositions and methods for providing RNA Interference (RNAi) vectors comprising maize lignin biosynthesis enzymes for altering lignin content of plants. Specifically, plants comprising RNAi maize lignin vectors for reducing or altering lignin content are provided for reducing pretreatment costs of biofuel production. Additionally, RNAi maize lignin vectors are provided for altering cellulose production in plants for reducing pretreatment costs of plant biomass processing by increasing amounts of fermentable sugars. | 2008-09-04 |
| 20080213872 | Disposable and Removable Nucleic Acid Extraction and Purification Cartridges For Automated Flow-Through Systems - Removable cartridges are used on automated flow-through systems for the purpose of extracting and purifying genetic material from complex matrices. Different types of cartridges are paired with specific automated protocols to concentrate, extract, and purifying pathogenic or human genetic material. Their flow-through nature allows large quantities sample to be processed. Matrices may be filtered using size exclusion and/or affinity filters to concentrate the pathogen of interest. Lysed material is ultimately passed through a filter to remove the insoluble material before the soluble genetic material is delivered past a silica-like membrane that binds the genetic material, where it is washed, dried, and eluted. Cartridges are inserted into the housing areas of flow-through automated instruments, which are equipped with sensors to ensure proper placement and usage of the cartridges. Properly inserted cartridges create fluid- and air-tight seals with the flow lines of an automated instrument. | 2008-09-04 |
| 20080213873 | Laboratory Apparatus with Two Cabinets - A laboratory apparatus for use in IVF treatment of infertility comprises first ( | 2008-09-04 |
| 20080213874 | WEIGHT MEASUREMENTS OF LIQUIDS IN FLEXIBLE CONTAINERS - Articles and methods for measuring weight of a liquid in a disposable bag bioreactor are presented. In certain embodiments, bioreactor systems described herein include a supported container (e.g., a flexible bag in a reusable housing) for containing a liquid, and at least two pressure indicating sensors operatively associated with the container. A first pressure indicating sensor can be placed near the bottom of the container to measure the total downward force within the container, including the liquid head in the container and the gas pressure above the liquid. The second pressure indicating sensor can be placed near the top of the container to measure only the pressure at the top of or above the liquid. Signals from the pressure indicating sensors can be directed to a control system that receives the signals and calculates a difference between the signals. This difference can be used to determine a volume or a weight of the liquid in the container. Advantageously, real-time weight measurements can be obtained while the system is in operation and continuous flow processes can be monitored. Moreover, in some embodiments, the pressure indicating sensors are isolated from contact with any fluid (e.g., liquid) in the container and, therefore, do not require cleaning after processing of each batch of reactants. Contamination of the process fluid by contact with the pressure indicating sensors can also be avoided. | 2008-09-04 |
| 20080213875 | ASSAY DEVICES AND METHODS - A device for determining an assay result may include a test strip, a light source system, a light detection system, and a processor. | 2008-09-04 |
| 20080213876 | Composting Apparatus - A composting apparatus is disclosed which comprises a container ( | 2008-09-04 |
| 20080213877 | Filtration Device For Biological Samples - An open-topped container ( | 2008-09-04 |
| 20080213878 | Novel human membrane proteins and polynucleotides encoding the same - Novel human polynucleotide and polypeptide sequences are disclosed that can be used in therapeutic, diagnostic, and pharmacogenomic applications. | 2008-09-04 |
| 20080213879 | Chimeric GFP-aequorin as bioluminescent Ca++ reporters at the single cell level - A modified bioluminescent system comprising a fluorescent molecule covalently linked with a photoprotein, wherein said link between the two proteins has the function to stabilize the modified bioluminescent system and allowing the transfer of the energy by Chemiluminescence Resonance Energy Transfer(CRET). | 2008-09-04 |
| 20080213880 | CHIMERIC T1R1 TASTE RECEPTOR ENCODING NUCLEIC ACID SEQUENCES AND VECTORS - Newly identified mammalian taste-cell-specific G protein-coupled receptors, and the genes and cDNA encoding said receptors are described. Specifically, T1R G protein-coupled receptors active in taste signaling, and the genes and cDNA encoding the same, are described, along with methods for isolating such genes and for isolating and expressing such receptors. Methods for representing taste perception of a particular tastant in a mammal are also described, as are methods for generating novel molecules or combinations of molecules that elicit a predetermined taste perception in a mammal, and methods for simulating one or more tastes. Further, methods for stimulating or blocking taste perception in a mammal are also disclosed. | 2008-09-04 |
| 20080213881 | Isolated nucleic acid molecules which encode T cell inducible factors (TIFs), the proteins encoded, and uses thereof - The invention involves isolation of nucleic acid molecules, the expression of which are upregulated by interleukin-9. The amino acid sequences of the proteins which correspond to the nucleic acid molecules show some structural features of cytokines. In addition to the nucleic acid molecules and the proteins, various uses of the molecules are disclosed. The molecules are referred to as T cell inducible factors. | 2008-09-04 |
| 20080213882 | Method for Isolating Dermal Papilla Cells and Uses Thereof - The present invention relates to dermal papilla-specific markers and a method for isolating dermal papilla cells using the same. Dermal papilla cells isolated in accordance with the method of the invention and treated with BMP6 are useful for promoting hair growth. | 2008-09-04 |
| 20080213883 | SILICA NANOPARTICLES IN BASIC AMINO ACID-SILICA SOLS - In general, in one aspect, the invention features a method that includes preparing a mixture comprising water, a basic amino acid, and a metal oxide precursor under conditions which result in the formation of metal oxide nanoparticles from the metal oxide precursor. | 2008-09-04 |
| 20080213884 | Vectors for tissue-specific replication - The invention generally relates to targeted gene therapy using recombinant vectors and particularly adenovirus vectors. The invention specifically relates to replication-conditional vectors and methods for using them. Such vectors are able to selectively replicate in a target tissue to provide a therapeutic benefit from the presence of the vector per se or from heterologous gene products expressed from the vector and distributed throughout the tissue. In such vectors, a gene essential for replication is placed under the control of a heterologous tissue-specific transcriptional regulatory sequence. Thus, replication is conditioned on the presence of a factor(s) that induces transcription or the absence of a factor(s) that inhibits transcription of the gene by means of the transcriptional regulatory sequence with this vector, therefore, a target tissue can be selectively treated. | 2008-09-04 |
| 20080213885 | COMPOSITION AND METHOD FOR ENABLING PROLIFERATION OF PLURIPOTENT STEM CELLS - The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, for culturing stem cells in vitro in an undifferentiated state. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or laminin-5 (laminin-322), or their functional domains, the embryonic stem cells proliferated and maintained their pluripotency. | 2008-09-04 |
| 20080213886 | Albumin fusion proteins - The present invention encompasses albumin fusion proteins. Nucleic acid molecules encoding the albumin fusion proteins of the invention are also encompassed by the invention, as are vectors containing these nucleic acids, host cells transformed with these nucleic acids vectors, and methods of making the albumin fusion proteins of the invention and using these nucleic acids, vectors, and/or host cells. Additionally the present invention encompasses pharmaceutical compositions comprising albumin fusion proteins and methods of treating, preventing, or ameliorating diseases, disorders or conditions using albumin fusion proteins of the invention. | 2008-09-04 |
| 20080213887 | METHODS AND COMPOSITIONS FOR CRYOPRESERVING OOCYTES - Disclosed are methods and compositions useful for cryopreserving oocytes and, in particular, mammalian oocytes such as human oocytes. | 2008-09-04 |
| 20080213888 | NEURAL PRECURSOR CELLS, METHOD FOR THE PRODUCTION AND USE THEREOF IN NEURAL DEFECT THERAPY - The invention relates to isolated and purified neural precursor cells, to methods for the generation of such precursor cells in unlimited quantities from embryonic stem cells, and to their use for the therapy of neural defects, particularly in mammals, preferably in human beings, and for the generation of polypeptides. | 2008-09-04 |
| 20080213889 | INHIBITORS OF PHOSPHODIESTERASES IN INFERTILITY - The present invention is directed to methods of increasing oocyte production in a mammal. More specifically, the specification describes methods and compositions for inducing follicular maturation using a PDE inhibitor. The inhibitor may be used alone at high doses. Alternatively, the follicular maturation is achieved by combining a low dose of FSH with the PDE inhibitor treatment. | 2008-09-04 |
| 20080213890 | Antibody-avidin fusion proteins as cytotoxic drugs - Methods and compositions for inducing apoptosis and/or inhibiting proliferation of cells. The method includes exposing the cells to a cytotoxic agent which is made up of a targeting moiety and an avidin moiety wherein the targeting moiety is capable of binding to one or more receptors located on the cells. The invention is based on the discovery that attaching an avidin moiety to non-toxic targeting moieties produces a cytotoxic agent which can be used to treat tumor cells both in vivo and in vitro. The present cytotoxic agent eliminates the use of biotinylated toxic drugs which previously have been conjugated to antibody-avidin targeting vehicles. | 2008-09-04 |
| 20080213891 | RNAi Agents Comprising Universal Nucleobases - One aspect of the present invention relates to an oligonucleotide agent comprising at least one universal nucleobase. In certain embodiments, the universal nucleobase is difluorotolyl, nitroindolyl, nitropyrrolyl, or nitroimidazolyl. In a preferred embodiment, the universal nucleobase is difluorotolyl. In certain embodiments, the oligonucleotide is double-stranded. In certain embodiments, the oligonucleotide is single-stranded. Another aspect of the present invention relates to a method of altering the expression level of a target in the presence of target sequence polymorphism. In a preferred embodiment, the oligonucleotide agent alters the expression of different alleles of a gene. In another preferred embodiment, the oligonucleotide agent alters the expression level of two or more genes. In another embodiment, the oligonucleotide agent alters the expression level of a viral gene from different strains of the virus. In another embodiment, the oligonucleotide agent alters the expression level of genes from different species. | 2008-09-04 |
| 20080213892 | SELF-RENEWAL OF NEURAL STEM CELLS IS PROMOTED BY WNT PROTEINS - Mammalian neural progenitor or stem cells are expanded in vitro by culture in the presence of one or more wnt polypeptides. The expanded cells substantially maintain their original phenotype including the ability to give rise to multiple types of differentiated cells. | 2008-09-04 |
| 20080213893 | ISOLATION OF NEURAL STEM CELLS USING GANGLIOSIDES AND OTHER SURFACE MARKERS - During the growth and study of NSCs, a range of molecules present on the surface of multipotent neural stem and progenitor cells (NSCs) were identified. These markers were identified using a number of human and murine neural stem cell lines, including retinal stem cells (RSCs). The NSC-specific markers identified included gene products as well as non-protein molecules and sugar epitopes not directly coded in the genome. Together with surface markers which were determined to be absent from the surface of hNSCs, the molecules described herein provide a means to enrich for neural stem cells, or neural progenitor subpopulations, particularly using combinatorial cell sorting strategies. These same molecules also represent targets for pharmacological manipulation of NSC populations and subpopulations, both in vivo and ex vivo. Furthermore, these molecules provide potential targets for therapeutic manipulation of other neural precursor-related cell types including malignant conditions as well as other diseases originating from, or preferentially affecting, various uncommitted or replication-competent cell types. | 2008-09-04 |
| 20080213894 | Disposable Tubing Set for Use with a Cell Expansion Apparatus and Method for Sterile Sampling - A disposable apparatus for cell expansion, having at least one bioreactor. The bioreactor has a cellular growth area and a supply area, the cellular growth area being separated from said supply area by a membrane. A fluid recirculation path in fluid communication with the cellular growth area allows for hermetically removing a sample containing cellular matter. This may comprise an elongated tube, or a plurality of parallel tube segments. The parallel tube segments have inflow ends and outflow ends, and the inflow ends are joined at a first common juncture and the outflow ends are joined at a second common juncture. The common junctures may comprise valves. | 2008-09-04 |
| 20080213895 | Method for culturing dendritic cells (DC) and cytokine-induced killer cells (D-CIK) and applications thereof - A mass production of dendritic cells (DC) and cytokine-induced killer cells (D-CIK) cells from the umbilical cord blood or peripheral blood via the activation with a cellular activator “Koyo” derived from the purified tumor cells during the aggregation and proliferation of the purified tumor cells is provided. A preparation of a vaccine with the dendritic cells (DC) and D-CIK cells is used to improve the symptoms of infectious diseases, cancers, metastasised cancers and auto-immune disorders. | 2008-09-04 |
| 20080213896 | Methods for storing conifer somatic embryo germinants - In one aspect, the present invention provides methods of storing conifer somatic embryo germinants germinated oil a sterile germination medium for delayed transplanting into a growth medium. The methods of the invention comprise the steps of: (a) placing the germinants while still on sterile germination medium into a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months, said germinants comprising a visible, well-defined epicotyl and radical; and (b) placing the germinants in water for a time greater than about 1 hour at a temperature below about 24° C. prior to transplantation into growth medium. | 2008-09-04 |
| 20080213897 | Methods for storing conifer somatic embryo germinants - In one aspect, the present invention provides methods of storing conifer somatic embryo germinants germinated on a sterile germination medium for delayed transplanting into a growth medium. The methods of the invention comprise the steps of: (a) placing the germinants while still on sterile germination medium into a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months, said germinants comprising a visible, well-defined epicotyl and radicle; and (b) placing the germinants in water for a time greater than about 1 hour at a temperature below about 24° C. prior to transplantation into growth medium. | 2008-09-04 |
| 20080213898 | COMPOSITIONS AND METHODS FOR NUCLEIC ACID DELIVERY - Compositions and methods are described for non-viral nucleic acid delivery. A targeting peptide capable of mediating targeting to a cell or subcellular compartment is derivatized with a photoaffinity label. Following an ionic interaction with a polynucleotide, such as DNA, and photolysis, the bioactive peptide becomes covalently attached to the DNA. Upon contact with a cell, the peptide facilities uptake of the peptide-polynucleotide conjugate into the cell or subcellular compartment. Methods for using this system for delivery of structural genes, including reporter genes, and detection of expression using bioluminescence are also described. | 2008-09-04 |
| 20080213899 | Rotationally Oscillating Injector - A microinjection device is provided that includes an injection element defining a longitudinal axis, and that further includes a motor. The injection element is rotatable about the longitudinal axis by the rotational motor. The injection element is for penetrating a target, such as a cell. A microinjection system is provided that includes the microinjection device and a control unit. The control unit is for controlling a rotational amplitude and a frequency of oscillation of the injection element. A method for penetrating a target to facilitate injecting material therein is provided that includes providing the material to an injection element, contacting the target with a distal end of the injection element, rotating the injection element about a longitudinal axis to form a hole in the target, and penetrating the target with the injection element via the hole formed in the target. A method for performing intra-cytoplasmic sperm injection is provided that includes providing a solution comprising sperm to an injection element, contacting an oocyte with a distal end of the injection element, rotating the injection element alternately clockwise and counterclockwise about a longitudinal axis to form a hole in the oocyte, penetrating the oocyte with the distal end of the injection element via the hole formed in the oocyte, and expelling the solution comprising sperm into the penetrated oocyte. | 2008-09-04 |
| 20080213900 | Engineered Protein Kinases Which Can Utilize Modified Nucleotide Triphosphate Substrates - Engineered protein kinases which can utilize modified nucleotide triphosphate substrates that are not as readily utilized by the wild-type forms of those enzymes, and methods of making and using them. Modified nucleotide triphosphate substrates and methods of making and using them. Methods for using such engineered kinases and such modified substrates to identify which protein substrates the kinases act upon, to measure the extent of such action, and to determine if test compounds can modulate such action. Also Engineered forms of multi-substrate enzymes which covalently attach part or all of at least one (donor) substrate to at least one other (recipient) substrate, which engineered forms will accept modified substrates that are not as readily utilized by the wild-type forms of those enzymes. Methods for making and using such engineered enzymes. Modified substrates and methods of making and using them. Methods for using such engineered enzymes and such modified substrates to identify the recipient substrates the enzymes act upon, to measure the extent of such action, and to measure whether test compounds modulate such action. | 2008-09-04 |
| 20080213901 | Methods for producing heterologous polypeptides in trichothecene-deficient filamentous fungal mutant cells - The present invention relates to methods for producing a polypeptide, comprising: (a) cultivating a mutant of a parent filamentous fungal cell under conditions conducive for the production of the polypeptide, wherein (i) the mutant cell comprises a first nucleic acid sequence encoding the polypeptide and a second nucleic acid sequence comprising a modification of at least one of the genes involved in the production of a trichothecene and (ii) the mutant produces less of the trichothecene than the parent filamentous fungal cell when cultured under the same conditions; and (b) isolating the polypeptide from the cultivation medium. The present invention also relates to mutants of filamentous fungal cells and methods for obtaining the mutant cells. The present invention also relates to isolated trichodiene synthases and isolated nucleic acid sequences encoding the trichodiene synthases. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the nucleic acid sequences as well as methods for producing the trichodiene synthases. The present invention further relates to mutants cells comprising a marker-free modification of a gene, and methods for obtaining and using such mutant cells. | 2008-09-04 |
| 20080213902 | Slide Holder for Staining Procedures - Improvements in FISH staining employing a microscope slide holder which allows for batch processing of slides in the hybridization process. | 2008-09-04 |
| 20080213903 | AUTOMATIC ANALYZER AND THE ANALYZING METHOD USING THE SAME - An automatic analyzer which assures uniformity in mixing effects regardless of sample quantity and test item and thus produces analysis results with high repeatability. The analyzer includes a device for adding a conditioning liquid into a reaction chamber so that the quantity of liquid in the reaction chamber becomes a predetermined quantity at latest just before mixing. The conditioning liquid may be a diluent or physiological saline as used for dilution of a sample or any other special liquid that adjusts the properties such as viscosity, surface tension, etc. of liquid to be mixed. | 2008-09-04 |
| 20080213904 | Monitoring drug compliance, food-intake or toxin-intake using non-invasively-read labels - A system is disclosed for monitoring a property of an ingested or in-taken drug, food, drink or toxic substance, non-invasively or minimally invasively, which can also identify the subject person being monitored, if desired. The system comprises: a means of labeling the substance with a labeling media to have a useful signature indicative of, or bearing a relation to the property; a means to allow the signature to be read non-invasively or minimally invasively; and a means to identify, in any manner, who is being monitored. | 2008-09-04 |
| 20080213905 | Methods of assessing the need for and the effectiveness of therapy with antioxidants - The invention relates to diagnostic methods for assessing the need of a subject for treatment with an anti-oxidant, or alternatively, for determining the utilization efficiency and ultimate effectiveness of anti-oxidant therapy in subjects having been treated with antioxidants. More specifically, the methods of the present invention are particularly useful in prophylactic assessment of individuals at risk for developing diseases or conditions in which oxidative stress plays a role, such that an appropriate therapeutic regimen can be prescribed for that individual, thus leading to alternative therapies and/or life style changes. The invention further relates to methods for assessing the need for, the utilization efficiency and the effectiveness of therapy in subjects having received therapy with specific antioxidant and immune enhancing formulations. Kits are also provided for measuring the levels of markers of oxidative stress and immune cell numbers. | 2008-09-04 |
| 20080213906 | COMPOSITIONS AND METHODS FOR COMBINING PROTEIN PRECIPITATION AND SOLID PHASE EXTRACTION - A composition, method and device for the preparation of biological samples for subsequent LC-MS analysis using a combined and concurrent protein precipitation and solid phase extraction (SPE) process is described. Through an integrated combination of protein precipitation, filtration, and SPE using a novel zirconia-coated chromatographic media, interfering compounds, such as proteins and phosphate-containing compounds, are eliminated from the biological samples, affording a higher degree of analyte response during LC-MS analysis. | 2008-09-04 |
| 20080213907 | Protein markers for the diagnosis and prognosis of ovarian and breast cancer - Plasma samples of ovarian and breast cancer patients were used to search for markers of cancer, using two-dimensional gel electrophoresis and MALDI TOF mass spectrometry. Truncated forms of cytosolic serine hydroxymethyl transferase (cSHMT), T-box transcription factor 3 (Tbx3) and utrophin were aberrantly expressed in samples from cancer patients, as compared to samples from noncancer cases. Aberrant expression of proteins was validated by immunoblotting of plasma samples with specific antibodies to cSHMT, Tbx3 and utrophin. A cohort of 79 breast and 39 ovarian cancer patients, and 31 individuals who were either healthy or had noncancerous conditions was studied. We observed increased expression of truncated cSHMT, Tbx3 and utrophin in plasma samples obtained from patients at early stages of disease. The results indicate that cSHMT, Tbx3, utrophin and truncated forms thereof can be used as components of multiparameter monitoring of ovarian and breast cancer. | 2008-09-04 |
| 20080213908 | FLAME DETECTOR - Improved operating modes of a micro counter-current flame ionization detector (μFID) are demonstrated. By operating the flame inside the end of a capillary gas chromatography (GC) column, the effective cell volume enclosing the flame is considerably reduced and results in significantly lower gas flows being required to produce optimal sensitivity from the stable flame. In a post-column μFID arrangement, a very lean flame is now situated on the end of a stainless steel capillary delivering 10 mL/min of hydrogen, which is opposed by a counter-current flow of only 20 mL/min of oxygen. The μFID detection limit obtained in this stable, oxygen-rich counter-current flame mode is 7×10 | 2008-09-04 |
| 20080213909 | PHARMACEUTICAL PACKAGING ASSAY - A pharmaceutical packaging assay for the determination of drug adsorption to the packaging surface(s) is described. The assay described utilizes fluorescent labeling of drugs in various formulations to determine the best packaging surface for drug stability as a function of adsorption. | 2008-09-04 |
| 20080213910 | METHOD FOR BLOCKING NON-SPECIFIC PROTEIN BINDING ON A FUNCTIONALIZED SURFACE - A method of blocking non-specific protein binding on surfaces, such as protein-coated biosensor surfaces. | 2008-09-04 |
| 20080213911 | Method of Analyzing C-Terminal Amino Acid Sequence of Peptide - An analyte peptide is selectively degraded sequentially by using an alkanoic anhydride (S | 2008-09-04 |
| 20080213912 | System and Methods for Stretching Polynucleotides - Apparatus and methods are described for achieving uniform stretching of polynucleotides in hybrid electrophoretic-gel, micro-constricting microfluidic channels. Polynucleotides in normally relaxed configurations are driven by an electric field along a microfluidic channel. The polynucleotides thread through a porous gel barrier formed in the channel and extend into a constriction where an electric field gradient exists. The combined action of the gel and field gradient acts to extend the polynucleotide configuration fully for direct linear analysis of the molecule. | 2008-09-04 |
| 20080213913 | COMBUSTION ANALYSIS APPARATUS AND METHOD - A method and apparatus for combustion analysing a sample in a combustion analyzer ( | 2008-09-04 |
| 20080213914 | METHOD AND SYSTEM FOR MEASURING RON AND MON VALUES FOR LIGHT DISTILLATES - A method and a system is disclosed for determining RON and/or MON values from constant volume combustion chamber apparatuses capable of producing pressure versus time combustion profiles having a fast combustion region and a slow combustion region, where data from the two regions is used to compute RON and/or MON values for light distillate fluid samples using a series expansion equation. | 2008-09-04 |
| 20080213915 | SYSTEM AND METHOD FOR THE MEASUREMENT OF MULTIPLE FLUORESCENCE EMISSIONS IN A FLOW CYTOMETRY SYSTEM - A system and method for the measurement of multiple fluorescence emissions in a flow cytometry system is disclosed where each excitation light source is modulated with a different frequency. A single detector is used to collect the fluorescent emissions excited by all light sources, and the emissions are segregated using Fourier Transform techniques. Systems and methods for the correction of inter-beam coincidence are also disclosed. | 2008-09-04 |
| 20080213916 | Allosterically Catalyzed Signal Amplification in Chemical and Biological Sensing - Coordination complexes having at least two structural conformations are disclosed. The coordination complexes contain at least one metal center and at least one hemi-labile ligand, and change structural conformations due to the presence or absence of allosteric effectors. Methods of detecting an analyte using the coordination complexes are also disclosed. | 2008-09-04 |
| 20080213917 | LUMINESCENT MACROCYCLIC LANTHANIDE COMPLEXES - The present invention provides a novel class of macrocyclic compounds as well as complexes formed between a metal (e.g., lanthanide) ion and the compounds of the invention. Preferred complexes exhibit high stability as well as high quantum yields of lanthanide ion luminescence in aqueous media without the need for secondary activating agents. Preferred compounds incorporate hydroxy-isophthalamide moieties within their macrocyclic structure and are characterized by surprisingly low, non-specific binding to a variety of polypeptides such as antibodies and proteins as well as high kinetic stability. These characteristics distinguish them from known, open-structured ligands. | 2008-09-04 |
