34th week of 2010 patent applcation highlights part 40 |
Patent application number | Title | Published |
20100216114 | METHOD AND APPARATUS FOR DETERMINATION OF COMPONENTS IN ROOT CROPS - The invention relates to a process for the determination of components in root crops as well as a device for performing the process. Root crops of a plot are prepared in such a way that becomes possible, using near-infrared spectroscopy, to determine the content of substances with a high analytical accuracy and short analysis time and wherein the recorded data concerning the identity and quantity of ingredients are representative of all root crops of a plot. | 2010-08-26 |
20100216115 | METHODS AND COMPOSITIONS FOR DIAGNOSTIC USE IN CANCER PATIENTS - Disclosed herein are methods and compositions useful for identifying therapies likely to confer optimal clinical benefit for patients with cancer. | 2010-08-26 |
20100216116 | VIRAL LATENCY MODEL - The invention relates generally to the field of virology. More particularly, the present invention relates to in vitro models for viral latency. In particular to latently infected cultures of primary and continuous cell lines, and to the use thereof in methods to identify anti-viral compounds. More in particular to identify compounds which are either able to modulate the induction of viral latency in the aforementioned cell cultures, or which are able to retain the viruses in the aforementioned cells in their latent form. Other aspects of the invention are directed to antiviral compounds identified using the models and methods of the present invention, as well as to the use thereof in treating latent infections such as for example latent Herpes Simplex Virus (HSV) infections. | 2010-08-26 |
20100216117 | OLIGONUCLEOTIDES, USE, METHOD OF DETECTION AND KIT FOR DIAGNOSING THE PRESENCE OF THE CHIKUNGUNYA VIRUS E1 GENE - The present invention concerns oligonucleotides intended to enable the amplification and the detection of a target sequence located in the E1 gene of the Chikungunya virus. These oligonucleotides are between 10 and 50 nucleotides in length and comprise at least one fragment of 10 consecutive nucleotides derived from the following sequences: SEQ ID No. 1: 5′-CTCTTACCGGGTTTGTTGC-3′ or SEQ ID No. 2: 5′-GCCTGGACACCTTTCGAC-3′, or the sequence complementary thereto. The invention also concerns the oligonucleotide which enables detection of the amplicons, the use of these oligonucleotides, a method of detection and a kit for diagnosing the presence of the E1 gene of the Chikungunya virus. The invention has a preferred use in the diagnostics field. | 2010-08-26 |
20100216118 | Method of Detection/Extraction, and Related Detection/Extraction Device - A method that uses an L-α-dipalmitoleoyl-phosphatidylcholine (DPPC) surfactant based device that reacts with a substance in a known manner, to detect a substance of interest or to extract a substance of interest from a material is provided. The principles of the present invention are particularly useful in detecting/measuring a substance that is harmful to a human, and also to extracting NACL from saltwater. | 2010-08-26 |
20100216119 | Diagnostic Methods for HIV Infection - The invention relates to a method of aiding the diagnosis of a human immunodeficiency virus infection in a subject, said method comprising (i) providing a sample from the subject (ii) determining the level of ps20 in said sample (iii) comparing the level of ps20 of (ii) with the level of ps20 in an uninfected reference sample, wherein a higher level of ps20 in the sample from the subject compared to the uninfected reference sample indicates an increased likelihood of human immunodeficiency virus infection in said subject. The invention also relates to methods for assessing susceptibility of a subject to human immunodeficiency virus infection. Most suitably the ps20 level is determined via binding by an anti-ps20 antibody such as the 107 antibody. The invention also relates to kits for use in said methods. | 2010-08-26 |
20100216120 | Rapid infectious virus assay - An assay to detect or quantify HIV infectious virus from clinically relevant cellular compartments, or reservoirs, in anti-retrovirally treated patients whose viral levels are low to undetectable is described. The method detects infectious virus in patients whose plasma viral loads are considered to be below the limit of current PCR based detection methods and thereby is more relevant for guiding treatment. A further advantage is that the method allows viral tropism to be directly determined in the presence of specific inhibitors of CCR5 or CXCR4. Drug sensitivity can also be directly determined without the need to laboriously recover patient virus by culture for extended time periods, a method that allows for viral selection or evolution, which is not desirable. Patient cells, like the blood mononuclear cells, or monocytes, are isolated and cultured in the presence of cytokines like CSF-1/M-CSF or GM-CSF. to promote their differentiation. Cells are activated with lectins, mitogenic antibodies, phorbol esters, Toll Receptor stimulation or inducers of NfKb or NFAT, followed by agents that induce viral release, like ATP or stimulation of autophagy with LiCl, spermidine, or rapamycin. A key aspect of the invention relates to the timing of the addition of these agents for optimal viral release. A further aspect of the invention relates to sensitive detection of released virus which can be accomplished by adding so-called reporter cells which are under control of the HIV TAT protein so that upon infection these cells synthesize proteins or enzymes that allow for the measurement of infectious particles. | 2010-08-26 |
20100216121 | Method for the detection of predisposition to high altitude pulmonary edema - The present invention encompasses methods, compositions and kits for the detection of susceptibility to high altitude pulmonary edema in a human subject. The present invention further encompasses isolated polynucleotides for the detection of susceptibility to high altitude pulmonary edema in a human subject. | 2010-08-26 |
20100216122 | ENZYMATIC NUCLEIC ACID SYNTHESIS: METHODS FOR DIRECT DETECTION OF TAGGED MONOMERS - Nucleotide triphosphate probes containing a molecular and/or atomic tag on a γ and/or β phosphate group and/or a base moiety having a detectable property are disclosed, and kits and method for using the tagged nucleotides in sequencing reactions and various assay. Also, phosphate and polyphosphate molecular fidelity altering agents are disclosed. | 2010-08-26 |
20100216123 | METHOD OF DETECTING MUTATION AND KIT USED IN THE SAME - A method of detecting a mutation is provided that uses Tm analysis and is excellent in detection sensitivity. A detection probe consisting of a polynucleotide complementary to a sequence to be detected containing a detection site that has been mutated and an inhibitory polynucleotide complementary to a sequence not to be detected containing the detection site that is unmutated are added to a sample containing a DNA to be detected in which the detection site has been mutated and a DNA not to be detected in which the detection site is unmutated, so that the detection probe is hybridized with the DNA. Then while the hybridization product between the DNA and the detection probe is heated, a signal variation associated with an increase in temperature is measured, then the signal variation is analyzed, and thereby a Tm value is determined, based on which the presence of the mutation is determined. | 2010-08-26 |
20100216124 | GENETIC MARKERS OF THE RISK OF DEVELOPING RESTENOSIS - Methods and kits for diagnosing the risk of developing restenosis after revascularization by implantation of stents based on the detection of single-nucleotide polymorphisms (SNPs). | 2010-08-26 |
20100216125 | NUCLEIC ACID ANALYSIS USING SEQUENCE TOKENS - The present invention provides methods and compositions for tagging nucleic acid sequence fragments, e.g., a set of nucleic acid sequence fragments from a single genome, with one or more unique members of a collection of oligonucleotide tags, or sequence tokens, which, in turn, can be identified using a variety of readout platforms. As a general rule, a given sequence token is used once and only once in any tag sequence. In addition, the present invention also provides methods for using the sequence tokens to efficiently determine variations in nucleotide sequences in the associated nucleic acid sequence fragments. | 2010-08-26 |
20100216126 | MICROFLUIDIC DEVICE - A microfluidic device comprising; i) an inlet; ii) a first layer comprising at least first and second current carrying structures, wherein the at least first and second current carrying structures each comprise a plurality of teeth, and wherein the teeth of the first and second current carrying structures are optionally offset such that the teeth of the first current carrying structure are positioned between the teeth of the second current carrying structure; iii) a second layer comprising a first microfluidic chamber in fluid communication with the inlet positioned above the at least first and second current carrying structures of the first layer; and iv) a third layer comprising at least third and fourth current carrying structures wherein the at least third and fourth current carrying structures each comprise a plurality of teeth, and wherein the teeth of the third and fourth current carrying structures are optionally offset such that the teeth of the third current carrying structure are positioned between the teeth of the fourth current carrying structure; and wherein the at least third and fourth current carrying structures are positioned in the third layer so as to be above the first microfluidic chamber and such that the teeth of the third current carrying structure are positioned substantially vertically above or offset from the teeth of the first current carrying structure and the teeth of the fourth current carrying structure are positioned substantially vertically above or offset from the teeth of the second current carrying structure; wherein the teeth have a stem having substantially elliptical tip. | 2010-08-26 |
20100216127 | PRIMER SET FOR USE IN DETECTION OF YEAST OF GENUS SACCHAROMYCES - An object of the present invention is to provide a primer set, which can accurately, rapidly and simply identify yeast species of genus | 2010-08-26 |
20100216128 | METHODS FOR ANALYZING AGRICULTURAL AND ENVIRONMENTAL SAMPLES - The present invention generally relates methods for analyzing agricultural and/or environmental samples using liquid bridges. In certain embodiments, the invention provides a method for analyzing an agricultural sample for a desired trait including obtaining a gene or gene product from an agricultural sample, in which the gene or gene product is in a first fluid; providing a liquid bridge for mixing the gene or gene product with at least one reagent to form a mixed droplet that is wrapped in an immiscible second fluid; and analyzing the mixed droplet to detect a desired trait of the agricultural sample. | 2010-08-26 |
20100216129 | GENETIC LOCI ASSOCIATED WITH FUSARIUM SOLANI TOLERANCE IN SOYBEAN - The invention relates to methods and compositions for identifying soybean plants that are tolerant, have improved tolerance or are susceptible to | 2010-08-26 |
20100216130 | SINGLE NUCLEOTIDE POLYMORPHISMS ASSOCIATED WITH AMYOTROPHIC LATERAL SCLEROSIS - Methods for determining the genetic predisposition of a human subject to developing ALS are provided herein. These methods include methods for determining the genetic predisposition to any form of ALS, as well as specific methods for determining the genetic predisposition to early onset, late onset, bulbar onset and limb onset ALS. The method can detect amyotrophic lateral sclerosis in a human subject or a specific form of ALS in the subject (early onset, late onset, bulbar onset or limb onset). The method can also detect the risk of developing amyotrophic lateral sclerosis (ALS) in a human subject. The methods utilize the detection of one or more haplotype bocks comprising tag single nucleotide polymorphisms (SNPs). In several embodiments, the methods including detecting the presence of one or more tag SNPs. | 2010-08-26 |
20100216131 | GENE EXPRESSION PROFILING OF ESOPHAGEAL CARCINOMAS - The present invention generally regards gene expression profiling of esophageal cancers, including localized esophageal cancers. In particular, gene expression for a particular group of genes identifies individuals that are either going to be responsive to cancer therapy, for example chemotherapy and/or radiation, or that are not going to be responsive to cancer therapy. Exemplary genes having such expression profiles include, for example, PERP, S100A2, and SPRR3. | 2010-08-26 |
20100216132 | METHODS AND COMPOSITIONS FOR DIRECT DETECTION OF DNA DAMAGE - The present invention is a method for detecting the extent of DNA damage in a subject suspected of having DNA damage wherein the damage results in the formation of aldehyde moieties in DNA comprising, obtaining a DNA sample from the subject, combining the DNA sample with a fluorescent, chromogenic, pro-fluorescent or pro-chromogenic hydrazine compound to from a fluorescent DNA, detecting the presence of the fluorescent DNA by monitoring the fluorescent emission and quantitating the fluorescent emission thereby determining the extent of DNA damage in the subject. | 2010-08-26 |
20100216133 | POLYNUCLEOTIDES FOR USE AS TAGS AND TAG COMPLEMENTS, MANUFACTURE AND USE THEREOF - A family of minimally cross-hybridizing nucleotide sequences, methods of use, etc. A specific family of 210 24 mers is described. | 2010-08-26 |
20100216134 | POLYNUCLEOTIDES FOR USE AS TAGS AND TAG COMPLEMENTS, MANUFACTURE AND USE THEREOF - A family of minimally cross-hybridizing nucleotide sequences, methods of use, etc. A specific family of 210 24mers is described. | 2010-08-26 |
20100216135 | POLYNUCLEOTIDES FOR USE AS TAGS AND TAG COMPLEMENTS, MANUFACTURE AND USE THEREOF - A family of minimally cross-hybridizing nucleotide sequences, methods of use, etc. A specific family of 210 24mers is described. | 2010-08-26 |
20100216136 | METHOD FOR IDENTIFYING A PORK CONTENT IN A FOOD - Pork-specific PCR assay is performed for Halal authentication, by detecting porcine DNA in food products. DNA from raw meat samples is extracted. The extracted DNA is tested using primers that react by amplifying pork DNA but not beef and chicken DNA. The real-time PCR assay is sensitive with a low detection limit when using samples that can be obtained from food products. The methods described herein can have a sensitivity threshold as low as 0.001 ng pork DNA or lower, whereas convention techniques typically do not have a detection limit lower than 0.1 ng pork DNA. | 2010-08-26 |
20100216137 | Gene Expression Profiling for Identification, Monitoring and Treatment of Ovarian Cancer - A method is provided in various embodiments for determining a profile data set for a subject with ovarian cancer or conditions related to ovarian cancer based on a sample from the subject, wherein the sample provides a source of RNAs. The method includes using amplification for measuring the amount of RNA corresponding to at least 1 constituent from Tables 1-5. The profile data set comprises the measure of each constituent, and amplification is performed under measurement conditions that are substantially repeatable. | 2010-08-26 |
20100216138 | METHOD FOR DNA BREAKPOINT ANALYSIS - The present invention relates to a method for identifying a DNA breakpoint and agents for use therein. More particularly, the present invention provides a method for identifying a gene translocation breakpoint based on the application of a novel multiplex DNA amplification technique. The method of the present invention facilitates not only the identification of the break-point position but, further, enables the isolation of the DNA segment across which the breakpoint occurs. This provides a valuable opportunity to conduct further analysis of the breakpoint region, such as to sequence across this region. The method of the present invention is useful in a range of applications including, but not limited to, providing a routine means to characterise the gene break-point associated with disease onset in a patient and thereby enable the design of patient specific probes and primers for ongoing monitoring of the subject disease condition. In addition to monitoring the progression of a condition characterised by the existence of the breakpoint, there is also enabled assessment of the effectiveness of existing therapeutic drugs and/or new therapeutic drugs and, to the extent that the condition is a neoplasm, prediction of the likelihood of a subject's relapse from a remissive state. | 2010-08-26 |
20100216139 | METHODS, COMPOSITIONS, AND DEVICES UTILIZING MicroRNA TO DETERMINE PHYSIOLOGICAL CONDITIONS - Methods, compositions, and devices are disclosed which use microRNA to detect, predict, treat, and monitor physiological conditions such as disease or injury. microRNA are isolated and their differential expression is measured to provide diagnostic information. This information may then be utilized for evaluation and/or treatment purposes. | 2010-08-26 |
20100216140 | Novel Human Dickkopf-Related Protein and Nucleic Acid Molecules and uses Therefor - Novel Dkk and Dkk-related polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length Dkk and Dkk-related proteins, the invention further provides isolated fusion proteins, antigenic peptides and antibodies. The invention also provides Dkk and Dkk-related nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced and non-human transgenic animals in which a Dkk and Dkk-related gene has been introduced or disrupted. Diagnostic, screening and therapeutic methods utilizing compositions of the invention are also provided. | 2010-08-26 |
20100216141 | COMPOSITIONS AND METHODS TO DETECT LEGIONELLA PNEUMOPHILA NUCLEIC ACID - Compositions are disclosed as nucleic acid sequences that may be used as amplification oligomers, including primers, capture probes for sample preparation, and detection probes specific for | 2010-08-26 |
20100216142 | MICRORNA BIOMARKERS IN LUPUS - The present invention provides methods of screening a subject for systemic lupus erythematosus (SLE), comprising detecting an increase in an amount of one or more markers associated with SLE in a biological sample from the subject, wherein the one or more markers is selected from the group consisting of miR-16-1, miR-16-2, miR-223, let7a-1, let7a-2, let7a-3, let 7c, let7g, and any combination thereof, whereby detection of the increase in the amount of the one or more markers identifies the subject as having SLE. The invention further provides methods of screening a subject for SLE comprising detecting a decrease in miR-95 in a biological sample from the subject, whereby detection of the decrease in the amount of miR-95 identifies the subject as having SLE. | 2010-08-26 |
20100216143 | Apparatus and Method for Differentiating Multiple Fluorescence Signals by Excitation Wavelength - An apparatus and method are provided for differentiating multiple detectable signals by excitation wavelength. The apparatus can include a light source that can emit respective excitation light wavelengths or wavelength ranges towards a sample in a sample retaining region, for example, in a well. The sample can contain two or more detectable markers, for example, fluorescent dyes, each of which can be capable of generating increased detectable emissions when excited in the presence of a target component. The detectable markers can have excitation wavelength ranges and/or emission wavelength ranges that overlap with the ranges of the other detectable markers. A detector can be arranged for detecting an emission wavelength or wavelength range emitted from a first marker within the overlapping wavelength range of at least one of the other markers. | 2010-08-26 |
20100216144 | ANALYSIS OF DNA SAMPLES - The invention provides an improved method for obtaining information about DNA analysis of samples of uncertain origin by establishing the likelihood that they arose in certain manners compared with other possible manners. In this way all of the analysis information is taken into account and likelihood ratios are provided to express the results. The invention is particularly useful in analysing small DNA samples or DNA samples where the contribution from one or more sources is small. | 2010-08-26 |
20100216145 | Assay for Detecting Circulating Free Nucleic Acids - This invention is directed, inter alia, to methods and kits for rapid, easy and cost-effective methods of all free nucleic acid quantification in inter alia, biological fluid samples. | 2010-08-26 |
20100216146 | Methods and Kits for Hybridizing Multiple PNA Probe Panels to Nucleic Acid Samples - Described herein are methods and kits that employ multiple probe sets in combination with sequential steps of hybridization analysis for multiplex analysis and/or detection of nucleic acids having one or more distinguishable target sequences. | 2010-08-26 |
20100216147 | SEQUENCE-SPECIFIC LARGE VOLUME SAMPLE PREPARATION METHOD AND ASSAY - Methods of selectively and rapidly identifying target nucleic acid molecules in large volumes of collection media where the target is present in a low concentration are disclosed. The methods can be used to identify, isolate, purify, or enrich a nucleic acid molecule containing a specific target sequence from a sample of nucleic acid molecules that do not contain the specific target sequence. Once isolated, the nucleic acid molecule containing a specific target sequence may be amplified or used in a variety of detection assays. | 2010-08-26 |
20100216148 | NOVEL TUMOR MARKER - The present invention concerns a gene product largely homologous to the epithelial growth factor receptor (EGFR). It further refers to mRNA coding for such epithelial growth factor receptor. The present invention provides such an epithelial growth factor receptor which is characterized in that either exons 12 to 14 or exons 12 to 15 are deleted. These novel variants of the epithelial growth factor receptor can be used for a diagnosis, stratification, therapy guidance of a tumor or therapy guidance of tumor surgery. | 2010-08-26 |
20100216149 | SUSCEPTIBILITY GENES FOR AGE-RELATED MACULOPATHY (ARM) ON CHROMOSOME 10q26 - Allelic variations in the genes PLEKHA1 and LOC387715 are identified herein as risk factor for Age Related Maculopathy (ARM). A method is therefore provided for identifying a risk of development of ARM in an individual that comprises identification of allelic variations in PLEKHA1 and/or LOC387715. Related apparatus, such as an array, are identified as being useful in implementing those methods. | 2010-08-26 |
20100216150 | Method for Identifying a Compound That Modulates Telomerase Activity - The present invention embraces methods for identifying compounds that modulate the activity of telomerase. Compounds of the invention are identified by designing or screening for a compound which binds to at least one amino acid residue of the TRBD, “thumb,” “finger,” and/or “palm” domain; or FP-pocket, PT-pocket or Th-pocket of telomerase and testing the compound for its ability to modulate the activity of telomerase. | 2010-08-26 |
20100216151 | METHODS FOR DETECTING FETAL NUCLEIC ACIDS AND DIAGNOSING FETAL ABNORMALITIES - The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus. | 2010-08-26 |
20100216152 | Method for the Carry-Over Protection in DNA Amplification Systems Targeting Methylation Analysis Achieved by a Modified Pre-Treatment of Nucleic Acids - Particular aspects provide methods for specific amplification of template DNA in the presence of potentially contaminating PCR products from previous amplification experiments. Particular embodiments comprise, in a first step, contacting DNA with a bisulfite solution, which sulfonates unmethylated (but not methylated) cytosines, resulting in cytosine deamination and generation of sulfonated uracil. Such sulfonation protects the template nucleic acid from being a target for the enzyme uracil-DNA-glycosylase (UNG), whereas any contaminating DNA, which contains unprotected unsulfonated or desulfonated uracils, is degraded enzymatically while the UNG is active. After UNG treatment and inactivation thereof, the sulfonated uracil bases are converted into uracil by desulfonation. Such aspects have substantial utility for decontamination of nucleic acid samples; e.g., for avoiding amplification of ‘carry over products’ in the context of DNA methylation analysis. In further aspects, the inventive methods can be generally used as simplified methods of bisulfite treatment. | 2010-08-26 |
20100216153 | METHODS FOR DETECTING FETAL NUCLEIC ACIDS AND DIAGNOSING FETAL ABNORMALITIES - The invention generally relates to methods for detecting fetal nucleic acids and methods for diagnosing fetal abnormalities. In certain embodiments, the invention provides methods for determining whether fetal nucleic acid is present in a maternal sample including obtaining a maternal sample suspected to include fetal nucleic acids, and performing a sequencing reaction on the sample to determine presence of at least a portion of a Y chromosome in the sample, thereby determining that fetal nucleic acid is present in the sample. In other embodiments, the invention provides methods for quantitative or qualitative analysis to detect fetal nucleic acid in a maternal sample, regardless of the ability to detect the Y chromosome, particularly for samples including normal nucleic acids from a female fetus. | 2010-08-26 |
20100216154 | GENETIC POLYMORPHISMS ASSOCIATED WITH LIVER FIBROSIS, METHODS OF DETECTION AND USES THEREOF - The present invention is based on the discovery of genetic polymorphisms that are associated with liver fibrosis and related pathologies. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, including groups of nucleic acid molecules that may be used as a signature marker set, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 2010-08-26 |
20100216155 | Method and Kit for Identifying Antibiotic-Resistant Microorganisms - The invention provides a rapid sample-processing method for preparing hybridization reaction mixtures substantially depleted of RNA, and a method of identifying the methicillin-resistance status and vancomycin-resistance status of an organism. | 2010-08-26 |
20100216156 | CANCER MARKERS - Provided are previously uncharacterized markers of cancers, for example colorectal cancers, and uses of these as diagnostic and prognostic markers of cancers, and in particular colorectal cancers. The markers are SEQ ID NO:1—hnRNP-K; SEQ ID NO:2—HMG-1; SEQ ID NO:3—proteasome subunit alpha type 1; SEQ ID NO:4—bifunctional purine biosynthesis protein; SEQ ID NO:5—STI1; SEQ ID NO:6—annex in IV; SEQ ID NO:7—60 kDa heat shock protein; SEQ ID NO:8—T complex protein 1 beta subunit; SEQ ID NO:9—T complex protein 1 epsilon subunit; SEQ ID NO:10—mortalin; and SEQ ID NO:11—TER-ATPase. The invention further provides related methods and materials for the use of the markers in therapeutic intervention in colorectal and other cancers e.g. to specifically target neoplastic cells without causing significant toxicity in healthy tissues, and to provide methods for the evaluation of the ability of candidate therapeutic compounds to modulate the biological activity of cancerous cells from the colon, rectum and other tissues. | 2010-08-26 |
20100216157 | IMMUNOGLOBULIN PEPTIDES AGAINST HEATED BOVINE BLOOD - The present invention is related to immunoglobulin peptides that recognize a thermostable antigen from bovine blood. The invention also provides methods for determining the presence of bovine blood in a food sample or an animal feed sample. | 2010-08-26 |
20100216158 | DIAGNOSTIC TEST FOR HEAD AND FACIAL PAIN - A diagnostic test for a head and facial pain disorder in a human patient includes providing test strip containing one or more antibodies corresponding one or more biological markers associated with the disorder or with multiple disorders. A saliva sample is collected from the human patient and applied to the test strip. The test strip is subsequently evaluated for evidence of binding of one or more of the antibodies with any biological markers present in the saliva sample. The progression of the head and facial pain disorder in the patient may be measured by collecting and evaluating additional saliva samples over time and comparing any changes in the amount of binding activity between or among samples. | 2010-08-26 |
20100216159 | SWITCHABLE AFFINITY BINDERS - Methods and kits for binding and releasing biological targets, comprising, a binder comprising an environmentally reactive molecular switch that can switch between a high affinity state, to bind the target, to a low affinity state, to release the target. | 2010-08-26 |
20100216160 | METHOD FOR THE SCREENING PROTEASOME OF ACTIVITY-MODULATING AGENTS AND MEANS FOR CARRYIING OUT SAID METHOD - An in cellulo method for the screening of proteasome activity-modulating agents, includes the following steps of:
| 2010-08-26 |
20100216161 | Method for identifying protease inhibitors - The invention describes a method for assaying HCV NS3 protease activity using an NS3•4A protease molecule. The invention also provides a method for screening and identifying modulators of NS3 protease. | 2010-08-26 |
20100216162 | Immunological Method, Test Kit and Device for the Determination of the Analyte Content of a Sample - The present invention relates to immunological methods for the determination of the analyte content in samples as well as to test kits and devices for performing the methods of the invention. In particular, the present invention relates to the analysis of samples of breeding animals, such as raw milk and serum, for an assessment of desired characteristics of the animal(s) tested, such as pregnancy, optimum point of insemination or diseases, wherein said analysis is easy to handle, time efficient and can be carried out on-site. | 2010-08-26 |
20100216163 | LECTIN-BASED GLYCAN ASSAY - The invention relates to the use of deglycosylated detectors, especially secondary antibodies, for the determination of sugar structures of proteins, especially recombinant proteins. It further relates to the use of deglycosylated enzymes in the determination of sugar structures with the aid of an enzyme-substrate reaction; the invention further relates to a method of determining the sugar structures of proteins, a sugar determination kit and the use of said kit for the determination of sugar structures, especially of recombinant therapeutic proteins, preferably immunoglobulins. | 2010-08-26 |
20100216164 | MAMMALIAN PRICKLE GENE - The present invention provides the mPrickle gene which encodes a protein present in mammalian PSD fractions. The mPrickle protein is localized in synapses, and binds to the scaffold protein PSD-95. Precipitation of endogenous mPrickle using an anti-mPrickle antibody results in the coprecipitation of NMDA receptors, and thus, mPrickle can be used in drug delivery systems that target NMDA receptors. NMDA receptors are closely related to learning and memory, and are also suggested to be involved in mental disorders. Thus, in the future, mPrickle is expected to be applicable to the diagnosis and/or treatment of neurodegenerative diseases associated with learning/memory, such as mental deterioration and dementia. | 2010-08-26 |
20100216165 | USE OF SOLUBLE FORMS OF THE DESMOGLEIN I PROTEIN FOR THE PURPOSES OF SCREENING FOR ANTI-AGEING ACTIVE AGENTS - The invention relates to the use of one or more complexed or noncomplexed, soluble peptide form(s) of Desmoglein I, as a marker for evaluating the effectiveness of active agents and/or of treatments, in particular anti-ageing active agents and/or treatments, with regard to an epidermis. | 2010-08-26 |
20100216166 | TARGET ACTIVATED MICROTRANSFER - A device for performing target activated transfer that includes a mounting surface for mounting a tissue sample; and a light source positioned to substantially uniformly irradiate both stained and unstained regions of the tissue sample with light energy that activates the reagent to selectively adhere the stained regions to a transfer surface. Also described is an automated system for transferring tissue from a tissue sample to a transfer substrate. The system includes means for holding a tissue section that includes targets specifically stained with an absorptive stain thereby resulting in a stained tissue surface, and a flexible transfer film that includes a lower thermoplastic layer in sufficient thermal contact with the stained tissue surface; an irradiating assembly configured to provide a predetermined uniform light dose to the entire tissue section; and means for applying a constant pressure to the transfer film during irradiation. | 2010-08-26 |
20100216167 | METHODS FOR RELIEVING NEUROPATHIC PAIN BY MODULATING ALPHA 1G T-TYPE CALCIUM CHANNELS AND MICE LACKING ALPHA 1G T-TYPE CALCIUM CHANNELS - The present invention relates to a novel use of a transgenic mouse deficient in α1G T-type calcium channel as an animal model for the study of neuropathic diseases, more precisely, a novel use of a transgenic mouse having resistance against neuripathic pain as an animal model for the development of a therapeutic agent and a treatment method for human neuropathic diseases. The transgenic mouse deficient in α1G T-type calcium channel having resistance against neuropathic pain, provided by the present invention, can be effectively used for the development of a therapeutic agent and a treatment method for human neuropathic diseases. | 2010-08-26 |
20100216168 | METHODS FOR EVALUATING ANGIOGENIC POTENTIAL IN CULTURE - The present invention provides a method of evaluating the angiogenic potential of a tumor, and for predicting the efficacy of anti-angiogenic therapies on an individualized basis. The method of the invention involves preparing an angiogenic signature for malignant cells in culture by assaying for the presence or level of one or more angiogenesis-related factors selected from VEGF/VPF, IL8/CXCL8, TGF-β1, TGF-β2, TGF-β3, bFGF/FGF-2, EGF, PDGF-AA, PDGF-AA/BB, IP-10, and Flt-3 ligand. The angiogenic signature may be prepared from cultures maintained under normoxic and/or hypoxic environments. The invention may be used in conjunction with chemoresponse testing of anti-tumor agents, to predict or suggest a combination therapy for cancer patients. | 2010-08-26 |
20100216169 | Method for Detecting and Prognosing Pre-Cancerous Cells Using 5, 10, 15, 20-Tetrakis (Carboxyphenyl) Porphine - Presented is a method of prognosing a patient's response to a cancer therapy wherein prior to the therapy contacting a sample of cells from the patient's tissue or organ being treated for the cancer with a solution of TCPP to permit binding of the TCPP to components of the abnormal dysplastic or carcinomic cells, if any are present; detecting TCPP fluorescence in the sample, the presence of TCPP fluorescence being indicative that the sample contains dysplastic or carcinomic cells; at intervals during the therapy and subsequent to the therapy performing steps a-c on another sample of cells from the patient's tissue or organ being treated for the cancer; and determining if the percentage of abnormal pre-cancerous cells in the samples tested during and subsequent to the therapy are reduced as compared with the sample tested prior to the therapy, the reduction being prognostic of the patients response to the cancer therapy. | 2010-08-26 |
20100216170 | METHODS FOR IDENTIFYING IMMUNOBINDERS OF CELL-SURFACE ANTIGENS - The invention provides methods for identifying immunobinders, such as scFv antibodies, capable of specifically binding to cell surface antigens, and compositions identified according to said methods. | 2010-08-26 |
20100216171 | Reducing Time to Result for Blood Bank Diagnostic Testing - Methods for reducing time to result in blood bank diagnostic testing with an agitation device and a low ionic strength solution are disclosed. Specifically provided are methods for reducing incubation time for antigen-antibody reactions in an immunohematologic assay by subjecting the assay reactants to incubation with agitation and optionally additionally a low ionic strength diluent. | 2010-08-26 |
20100216172 | FLOW-BASED ENHANCEMENT OF SPECIFICITY FOR LABEL-FREE BIOCHEMICAL ASSAYS - A label-free biochemical assay, in which label-free interrogation of a target-receptor layer is performed while the target-receptor layer is subjected to a relatively strong flow of an analyte-containing fluid. The volumetric flow rate for the assay is selected based on calibration data corresponding to the target substance, which advantageously results in fewer and/or smaller false-positive signals corresponding to non-target substances compared to those produced with the fluid being stationary. In various embodiments, the label-free interrogation method can be electro-mechanical and/or optical. | 2010-08-26 |
20100216173 | CITRULLINATED FIBRIN-FILAGGRIN CHIMERIC POLYPEPTIDE CAPABLE OF DETECTING THE ANTIBODIES GENERATED IN RHEUMATOID ARTHRITIS - The present invention concerns a chimeric polypeptide, capable of detecting the antibodies generated in rheumatoid arthritis, comprising at least two citrulinated peptide subunits: (i) one derived from the α or β chain of the fibrin and (ii) a second derived from the filaggrin. In addition, the invention comprises an antigenic composition, a method and a kit for the diagnosis of rheumatoid arthritis, from the detection of the autoantibodies generated during the course of said disease. | 2010-08-26 |
20100216174 | METHOD OF EVALUATING THROMBOGENIC MICROPARTICLES - A method and a kit are disclosed for evaluating thrombogenic microparticles (MP). The principle is based on the evaluation of thrombin generation in platelet poor plasma (PPP) and in plasma made microparticle free (MPFP) by centrifugation or filtration. The difference in thrombin generation between PPP and MPFP correlates with number and activity of thrombogenic microparticles. Evaluating thrombin generation of different standardized amounts of MPs in standard MPFP allows to calculate the number of thrombogenic MP from the difference in thrombin generation between PPP and MPFP. | 2010-08-26 |
20100216175 | Condensate Glucose Analyzer - Systems and methods for analyzing glucose present in exhaled breath condensate (EBC). In certain embodiments, electrochemical- or coulometnc-based sensing technologies are used to analyze EBC for the presence and/or concentration of glucose. Based on the detected glucose m EBC, the subject invention provides systems and methods for non-invasive, accurate assessment of blood glucose levels. | 2010-08-26 |
20100216176 | NOVEL METHOD FOR SEQUENCE DETERMINATION USING NMR - The invention relates to methods for analyzing polysaccharides. In particular, compositional and sequence information about the polysaccharides are derived. Some methods use NMR in conjunction with another experimental method, such as, capillary electrophoretic techniques for the analysis. | 2010-08-26 |
20100216177 | METHOD AND KIT FOR UNIVERSAL VERIFICATION OF ENZYME ACTIVITY AND PROTEIN DIGESTION - A method of assessing enzyme function or protein stability is disclosed. A sample having a protein therein is contacted with an enzyme that is suspected of being capable of catalyzing digestion of the protein in order to form a mixture. First and second aliquots are removed from the mixture at first and second times, respectively, and protein digestion is evaluated using a detection reagent to calculate a change in absorbance between first and second aliquots. The change in absorbance provides an index of enzyme activity, enzyme capability, and protein stability. | 2010-08-26 |
20100216178 | METHOD OF STABILIZING HEME PROTEIN AND STORAGE SOLUTION THEREFOR - A method of stabilizing a hem protein which is effective against the denaturation and degradation of a hem protein typified by hemoglobin and a storage solution therefor. A method of stabilizing a hem protein and a storage solution therefor characterized in that an iminocarboxylic acid or its salt is made to coexist in a sample containing the hem protein, wherein the above-described iminocarboxylic acid is a compound represented by the following general formula (1) wherein R represents a hydrogen atom or a hydroxyl group; and X's represent each a hydrogen atom, an alkali metal or an ammonium group. | 2010-08-26 |
20100216179 | Method for detecting cancer and reagents for use therein - A method for detecting cancer includes providing a biomedical sample, performing a first adsorption step, performing a first desorption step, and performing a first discrimination step. The first adsorbing step includes immersing the biomedical sample into a first detection reagent having a first adsorbent that adsorbs onto the biomedical sample. The first desorption step includes immersing the biomedical sample into a first desorbing agent for a first period of time. The first discrimination step includes measuring an amount residual of first adsorbent adsorbed on the biomedical sample for identifying distribution of cancer cells within the biomedical sample. The present invention could be widely applied for detecting various cancers based on the differential physisorption of adsorbent. The present invention can provide a method for rapidly and non-invasively detecting cancers. | 2010-08-26 |
20100216180 | METHODS OF USING HALOGENATED PEPTIDES AS INTERNAL STANDARDS FOR LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY - Methods of using halogenated peptides as internal standards for liquid chromatography-mass spectrometry, and novel halogenated peptides useful for the same, are disclosed. In particular, methods of using halogenated peptides as internal standards in proteomic analyses, as well as methods of using halogenated peptides to conduct quality control assessments of and/or to calibrate liquid chromatography-mass spectrometry systems are disclosed. | 2010-08-26 |
20100216181 | METHODS AND COMPOSITIONS FOR THE DIFFERENTIATION OF STEM CELLS - The present invention provides methods and compositions for the production of hematopoietic progenitor cells or endothelial progenitor cells from human pluripotent stem cells using a defined cell culture medium without the need to utilize feeder cells or serum. In some embodiments, differentiation is accomplished using hypoxic atmospheric conditions. The defined medium of the present invention may contain growth factors and a matrix component. The hematopoietic progenitor cells may be further differentiated into cell lineages including red blood cells, macrophages, granulocytes, and megakaryocytes. The endothelial progenitor cells may be further differentiated into endothelial cells. Also disclosed are screening assays for identification of candidate substances that affect differentiation of pluripotent stem cells into progenitor cells. | 2010-08-26 |
20100216182 | Cyanine-Based Probe\Tag-Peptide Pair Fluorescence Protein Imaging and Fluorescence Protein Imaging Methods - A molecular probe comprises two arsenic atoms and at least one cyanine based moiety. A method of producing a molecular probe includes providing a molecule having a first formula, treating the molecule with HgOAc, and subsequently transmetallizing with AsCl | 2010-08-26 |
20100216183 | MICROBIAL DETECTION APPARATUS, MICROBIAL DETECTION METHOD, AND SAMPLE CONTAINER USED THEREIN - The sample container has a two-layer membrane filter comprising a first layer as an upper layer serving as a hydrophilic membrane filter and a hydrophobic membrane filter as an underlying second layer capable of filtering an aqueous solution without the use of a wetting agent and by means of a formed negative pressure. Using this sample container, a large amount of an aqueous sample solution is filtered by means of a negative pressure formed by a suction portion to capture microbes in the aqueous sample solution by the hydrophilic membrane filter. Then, the negative pressure is restored to normal pressure, and a microbial dissolution solution is then added to the membrane filter to retain the microbial dissolution solution for a given time on the hydrophobic membrane filter. Then, the microbial dissolution solution is dispensed to a reaction container containing a luminescent reagent, and luminescence is detected to detect the microbes. | 2010-08-26 |
20100216184 | PREPARATION OF CELL EXTRACT AND ITS APPLICATION FOR CELL-FREE PROTEIN SYSTHESIS - Disclosed is a process for simply preparing cell extracts for use as a catalyst of cell-free protein synthesis by centrifugation, which improves cost effectiveness and productivity of cell-free protein synthesis. Specifically, a conventional process for preparing cell extracts comprises the complicated steps, i.e. cell culture, cell lysis, high-speed centrifugation, pre-incubation, dialysis and the like. In comparison, the cell lysate just obtained by centrifugation is directly applied to protein synthesis, thereby providing higher producibility and more consistent productivity of protein than the conventional process. Further, the cell extracts are prepared by the simple process to reduce the protein production cost and time by about 60% and about 80%, respectively. | 2010-08-26 |
20100216185 | ENGINEERED VERSIONS OF POLYSIALYLTRANSFERASES WITH ENHANCED ENZYMATIC PROPERTIES - The invention relates to poly-sialyltransferse polypeptides with enhanced solubility and activity and methods of using the poly-sialyltransferases for production of poly-sialylated end products, e.g., oligosaccharides, glycoproteins and glycolipids. | 2010-08-26 |
20100216186 | NOVEL SESQUITERPENE SYNTHASE GENE AND PROTEIN - The invention relates to sesquiterpene synthases and methods for their production and use. Particularly, the invention provides nucleic acids comprising the nucleotide sequence of citrus valencene synthase (CVS) which codes for at least one CVS. The invention further provides nucleic acids comprising the nucleotide sequence coding for amino acid residues forming the tier 1 and tier 2 domains of CVS. The invention also provides for methods of making and using the nucleic acids and amino acids of the current invention. | 2010-08-26 |
20100216187 | AMINO ACID SEQUENCES THAT BIND TO A DESIRED MOLECULE IN A CONDITIONAL MANNER - The present invention relates to amino acid sequences that bind to serum proteins such as serum albumin; to compounds, proteins and polypeptides comprising or essentially consisting of such amino acid sequences; to nucleic acids that encode such amino acid sequences, proteins or polypeptides; to compositions, and in particular pharmaceutical compositions, that comprise such amino acid sequences, proteins and polypeptides; and to uses of such amino acid sequences, proteins and polypeptides, is essentially conditional on different physiological situations, e.g. is different under acidic condition than under pH-neutral condition. | 2010-08-26 |
20100216188 | USE OF CHICK BETA ACTIN GENE INTRON-1 - A method to use chick beta actin gene intron-1 or functional equivalent as a gene expression enhancer element or a gene expression “hot spot” sequence for constructing or reconstructing a mammalian expression vector for extremely high expression of recombinant proteins is disclosed. Composition of a set of extremely strong gene expression vectors is also disclosed. | 2010-08-26 |
20100216189 | SYNTHETIC REPETITIVE PROTEINS, THE PRODUCTION AND USE THEREOF - A repetitive protein having repetition units comprising the consensus sequence (I) | 2010-08-26 |
20100216190 | Novel Selection System - The present invention relates to a method of producing a recombinant protein comprising using a selection method other than antibiotics. In particular, it relates to a stable host/vector system based on the pyrC gene complementation designed to produce high level of heterologous recombinant protein in | 2010-08-26 |
20100216191 | Method for Manufacturing a Modified Peptide - The present invention relates to a method for manufacturing a modified polypeptide from a first polypeptide, said modified polypeptide exhibiting altered binding properties to a target molecule and/or having a different amino acid sequence compared to a first polypeptide comprising the steps of a) providing a first cell comprising a nucleic acid molecule encoding for a first fusion polypeptide, said first fusion polypeptide comprising at least one first polypeptide and a transcriptional activation domain, and comprising optionally a nucleic acid molecule encoding for a second fusion polypeptide, said second fusion polypeptide comprising the target molecule or a polypeptide domain binding the target molecule and a DNA binding domain, whereby the cell further comprises a reporter gene encoding a reporter polypeptide operably linked to an upstream transcriptional regulatory sequence comprising a DNA binding site as target for the at least one first polypeptide or optionally a DNA binding site for the DNA binding domain of the second fusion polypeptide, b) cultivating the cells of step a), c) identifying at least one cell expressing the reporter polypeptide, d) isolating at least one nucleic acid molecule encoding for at least one first polypeptide of the at least one cell identified in step c), e) modifying the at least one nucleic acid molecule of step d) by introducing at least one mutation thus obtaining at least one modified nucleic acid molecule encoding for at least one modified polypeptide, f) introducing the at least one modified nucleic acid molecule of step e) into at least one second cell comprising optionally a nucleic acid molecule encoding for a second fusion polypeptide, said second fusion polypeptide comprising the target molecule or a polypeptide domain binding the target molecule and a DNA binding domain, and g) repeating steps a) to f) at least twice until a nucleic acid molecule encoding for a modified polypeptide is obtained and isolated in step d) exhibiting predetermined altered binding properties to the target molecule compared to the at least one first polypeptide and/or having a different amino acid sequence compared to the first polypeptide, wherein in the repeating steps a) to d) the first polypeptide is exchanged with the modified polypeptide of step e). | 2010-08-26 |
20100216192 | TAILORED MULTI-SITE COMBINATORIAL ASSEMBLY - The present invention provides a novel method of producing a plurality of modified polynucleotides having different combinations of various mutations at multiple sites by a tailored multi-site combinatorial assembly, comprising adding at least two or at least three primers to a double stranded template polynucleotide in a single reaction mixture, wherein the primers are not overlapping, and wherein each of the primers comprise at least one mutation different from the other primers, wherein at least one primer is a forward primer that can anneal to a minus strand of the template and at least one primer is a reverse primer that can anneal to a plus strand of the template, and subjecting the reaction mixture to a polymerase extension reaction to yield a plurality of extended modified polynucleotides from the at least three primers. The method can be performed without employing a ligation step prior to transforming the extended modified polynucleotides into a cell. The plurality of extended modified polynucleotides can be treated with an enzyme for destroying the template polynucleotide prior to transforming in to the cell. | 2010-08-26 |
20100216193 | Reaction chip, reaction method, temperature controlling unit for gene treating apparatus and gene treating apparatus - The reaction chip of the present invention has a plurality of recesses | 2010-08-26 |
20100216194 | SINGLE-CELL MRNA QUANTIFICATION WITH REAL-TIME RT-PCR - The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA comprising the steps of a) lysis of a cellular sample which is supposed to contain the target RNA with a lysis buffer comprising between 0.2 M and 1 M guanidine thiocyanate, b) diluting the sample to an extend such that guanidine thiocyanate is present in a concentration of about 30 to 50 mM, c) reverse transcribing in the presence of a mixture of first strand cDNA synthesis primers, the mixture consisting of oligo dT primers and random primers, and d) subjecting the sample to multiple cycles of a thermocycling protocol and monitoring amplification of the first strand cDNA in real time. | 2010-08-26 |
20100216195 | Enzymatic Production of Sucrose-6-Ester, an Intermediate for the Manufacturing of Halo Sugars... - A novel process is described for production of 6-acyl-sucrose comprising enzymatic acylation of sucrose by an esterifying agent including an organic acid in presence of a lipase or an esterase in a solvent in which the enzyme used is stable. Chlorinated sucrose, the high intensity sweetener trichlorogalactosucrose can be prepared by chlorination and deacylation of 6-acyl sucrose prepared by the process of this invention. | 2010-08-26 |
20100216196 | L-CYSTEINE-PRODUCING BACTERIUM AND A METHOD FOR PRODUCING L-CYSTEINE - The present invention provides a bacterium belonging to the family Enterobacteriaceae, which is able to produce L-cysteine and has been modified to increase the activity of the protein encoded by the dsbA gene. This bacterium is cultured in a medium, and L-cysteine, L-cystine, a derivative or precursor thereof or a mixture thereof can be collected from the medium. | 2010-08-26 |
20100216197 | MICROBIAL FERMENTATION-BASED PRODUCTION OF PHOSPHOLIPIDS CONTAINING LONG-CHAIN POLYUNSATURATED FATTY ACIDS AS THEIR CONSTITUENTS - The present invention relates to a method for producing a phospholipid containing a long-chain polyunsaturated fatty acid as a constituent (LCPUFA-PL), which comprises: (i) culturing a microorganism belonging to | 2010-08-26 |
20100216198 | METHOD FOR THE SYNTHESIS OF OMEGA-UNSATURATED FATTY ACIDS - The invention relates to a method for the synthesis of short-chain ω-unsaturated fatty acids of general formula CH | 2010-08-26 |
20100216199 | Production of long chain unbranched beta-poly(L-malic acid) by large scale Physarum cultivation and high-grade purification of the same - The invention is a large-scale production and purification of beta-poly(L-malic acid), biodegradable natural polyester of L-malic acid of molecular weight 30,000 to 300,000 from plasmodia of the Physarum family, especially Physarum polycephalum. This will replace previous laborious and uncontrolled production by bioreactor methods of high productivity and quality, especially to obtain pure polymer of high molecular weight. The described 20-liter bioreactor method can be scaled up for industrial purpose at optimal production and minimum growth time to prevent degradation The invention includes the method of isolation of polymalic acid of 100,000 Mn (number-averaged molecular weight) from the culture broth optimized towards high yields of extremely pure polymalic acid. The non-hygroscopic, water and organic solvent-soluble polymer is endotoxin and agglutinin free and ready to use in chemical syntheses. Because of its high molecular weight and purity it offers a wide spectrum of applications in pharmacy and medicine. | 2010-08-26 |
20100216200 | SYSTEMS AND METHODS FOR PRODUCING BIOFUELS AND RELATED MATERIALS - cells (American Type Culture Collection 700394 | 2010-08-26 |
20100216201 | Processes of Producing Fermentation Products - The invention relates to a process of fermenting plant material in a fermentation medium into a fermentation product using a fermenting organism, wherein one or more carbonic anhydrases are present in the fermentation medium. | 2010-08-26 |
20100216202 | Method And A System For The Pretreatment Of Lignocellulosic Material - The invention relates to a method and a system for the pretreatment of lignocellulosic material by thermal hydrolysis, in particular with a view to producing bioethenol, wherein the material is first admixed with water and is then passed to a reactor, in which the soaked material is subjected to a high temperature and a high pressure to such an extent that is it accessible for a subsequent treatment with enzymes, following which the treated material is separated into liquid part and a solid part, where the solid component is subsequently used for the production of bioethenol and enzymation and fermentation. Exclusively steam is used in the method and the corresponding system for heating, stirring and transport, as e.g. the transport through the various elements of the system is provided by a pressure difference between the compartments of the elements. The material ( | 2010-08-26 |
20100216203 | Algae bioreactor using submerged enclosures with semi-permeable membranes - Methods for producing hydrocarbons, including oil, by processing algae and/or other micro-organisms in an aquatic environment. Flexible bags (e.g., plastic) with CO | 2010-08-26 |
20100216204 | NUCLEIC ACID SEQUENCES TO PROTEINS INVOLVED IN TOCOPHEROL SYNTHESIS - Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell. | 2010-08-26 |
20100216205 | Reagents and Methods for Cyanobacterial Production of Bioplastics and Biomaterials - The present invention provides reagents and methods for biomaterial production from cyanobacteria. | 2010-08-26 |
20100216206 | Method for Preparing an Acellular Organic Tissue for Revitalisation and Device for Implementing Said Method - The invention concerns a method for preparing an acellular organic tissue for revitalisation by means of the reimplantation of living cells, said method involving the following stages: preparing the acellular tissue on an essentially flat surface, creating a plurality of holes on the surface of the tissue, distributed all over said surface and positioned so that they penetrate at least through a portion of the thickness of said tissue. The holes are suitable for containing the living cells when they are reimplanted. The invention also includes a device for implementing said method, characterized in that it comprises—one or more needles ( | 2010-08-26 |
20100216207 | Apparatus and method for growing algae by ionizing radiation - An apparatus is disclosed for growing algae by ionizing radiation. The apparatus includes a container for containing medium for the growth of algae, a filter disposed in the container, a 3-dimensional rack disposed in the container, an adherent element wound on the 3-dimension rack so that spores of the algae can be planted in the adherent element, a radiation element disposed in the container for irradiating the spores and a timepiece connected to the radiation element. | 2010-08-26 |
20100216208 | Multiple laminar flow-based particle and cellular separation with laser steering - The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage. | 2010-08-26 |
20100216209 | IMMOBILIZED LIPASE AND METHOD FOR PRODUCING THE SAME - Disclosed is an immobilized lipase which is improved in transesterification activity when compared with conventional immobilized lipases. Specifically disclosed is an immobilized lipase remarkably improved in transesterification activity, which is obtained by immobilizing an oil-in-water emulsion containing an enzyme having a lipase activity, a polymer emulsifier and a fat/oil to a carrier. | 2010-08-26 |
20100216210 | METHODS AND COMPOSITIONS FOR REPAIR OF CARTILAGE USING AN IN VIVO BIOREACTOR - Methods and compositions for the biological repair of cartilage using a hybrid construct combining both an inert structure and living core are described. The inert structure is intended to act not only as a delivery system to feed and grow a living core component, but also as an inducer of cell differentiation. The inert structure comprises concentric internal and external and inflatable/expandable balloon-like bio-polymers. The living core comprises the cell-matrix construct comprised of HDFs, for example, seeded in a scaffold. The method comprises surgically removing a damaged cartilage from a patient and inserting the hybrid construct into the cavity generated after the foregoing surgical intervention. The balloons of the inert structure are successively inflated within the target area, such as a joint, for example. Also disclosed herein are methods for growing and differentiating human fibroblasts into chondrocyte-like cells via mechanical strain. | 2010-08-26 |
20100216211 | FIBROUS MATS CONTAINING CHITOSAN NANOFIBERS - The invention relate to fibrous mats comprising chitosan nanofibers and, optionally, at least one filler material, at least one additive, or both. The invention also relates to methods of making same, and devices that include a fibrous mat comprising chitosan nanofibers. | 2010-08-26 |
20100216212 | ANTI-OBESITY AGENT AND ANTI-OBESITY FOOD - An anti-obesity agent containing, as an active ingredient, a microorganism which belongs to the species | 2010-08-26 |
20100216213 | Polypeptides Having Glucoamylase Activity and Polynucleotides Encoding Same - The present invention relates to polypeptides having glucoamylase activity and isolated polynucleotides encoding said polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. The invention also relates to the composition comprising a glucoamylase of the invention as well as the use such compositions for starch conversion processes, brewing, including processes for producing fermentation products or syrups. | 2010-08-26 |