32nd week of 2012 patent applcation highlights part 37 |
Patent application number | Title | Published |
20120202197 | EFFICIENT PROLIFERATION METHOD FOR A KUPFFER CELL AND USE THEREOF - The present invention has successfully established a mixed culture system capable of actively proliferating a Kupffer cell in a primary culture of a cell population derived from a liver. Additionally, the present invention has successfully established a novel production method for efficiently producing a large amount of highly purified Kupffer cells using this mixed culture system. | 2012-08-09 |
20120202198 | PROCESS FOR THE SYNTHESIS OF A CDNA IN A SAMPLE IN AN ENZYMATIC REACTION - This invention relates to a process for synthesis of a cDNA in a sample, in an enzymatic reaction, whereby the process comprises the steps: simultaneous preparation of a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, a buffer, at least one ribonucleotide, at least one deoxyribonucleotide, an anchor oligonucleotide; addition of a sample that comprises a ribonucleic acid; and incubation of the agents of the previous steps in one or more temperature steps, which are selected such that the first enzyme and the second enzyme show activity. The invention further relates to a reaction mixture that comprises a first enzyme with polyadenylation activity, a second enzyme with reverse transcriptase activity, optionally a buffer, optionally at least one ribonucleotide, optionally at least one deoxyribonucleotide, and optionally an anchor oligonucleotide. Moreover, the invention relates to a kit that comprises a corresponding reaction mixture. | 2012-08-09 |
20120202199 | METHOD FOR ADJUSTING AMPLIFICATION EFFICIENCY OF TARGET POLYNUCLEOTIDE - Disclosed is a method for adjusting the amplification efficiency of a target polynucleotide in the amplification of the target polynucleotide by PCR using primers (i) to (iii) below, the method comprising adjusting the amplification efficiency of the target polynucleotide by changing the quantity ratio of the primers (i) to (iii) below:
| 2012-08-09 |
20120202200 | METHODS FOR DETECTING HUMAN PAPILLOMA VIRUS-ASSOCIATED CANCERS - The present invention provides probes and methods of use thereof in the diagnosis and/or prognosis of certain types of cancers, particularly human papillomavirus (HPV)-associated cancers. The probes are designed for hybridization with genomic material in a manner indicative of one or more aberrations in the genetic material present in the test sample. The identified aberrations are biomarkers of HPV-associated cancer. The methods of the invention comprise contacting a sample to one or more probes, allowing any genetic material in the sample to hybridize to the genomic regions provided in the probes, analyzing the hybridizations, and analyzing the hybridizations to identify detected aberrations as biomarkers indicative of HPV-associated cancer progression. | 2012-08-09 |
20120202201 | DEVICE AND METHOD FOR ANALYSIS OF KIDNEY FAILURE - The invention provides a method for analysis of the status of kidney function in a sample of body fluid obtained from a patient who has not been diagnosed with acute kidney injury (AKI, also referred to as acute kidney failure) or in a sample of body fluid obtained from a patient who has been diagnosed with AKI, which analysis is suitable for evaluating the prospects for long-term survival, which method for analysis comprises or consists of determining the concentration of the microRNA-210 | 2012-08-09 |
20120202202 | METHODS FOR DETECTING RARE CIRCULATING CANCER CELLS USING DNA METHYLATION BIOMARKERS - Provided are new and improved methods for detecting circulating tumor cells and tumor cell DNA in patient blood or other biofluid samples. Particular aspects comprise three steps: DNA extraction from patient samples, DNA digestion with multiple selected methylation-sensitive enzymes, and target amplification by a conventional or a real-time PCR with specific probe and/or primers. Also provided are a total of 40 tumor-specific DNA methylation loci as biomarkers having substantial utility and specificity in major types of human malignancies including hematopoietic and solid tumors. | 2012-08-09 |
20120202203 | SINGLE PROBE, MULTIPLE TEMPERATURE, NUCLEIC ACID DETECTION METHODS, KITS, AND COMPOSITIONS - Provided herein are methods, kits, and compositions related to nucleic acid detection assays that allow discrimination of multiple target sequences with a single probe. In particular, provided herein are methods kits, and compositions that include single-probe target sequence discrimination where different target amplicons may have identical probe hybridization sequences by employing multiple temperature end-point signal probe detection. Also provided herein are methods, kits, and compositions for distinguishing between two or more target amplicons using multiple-temperature end-point probe detection. In certain embodiments, asymmetric PCR amplification methods are employed (e.g., LATE-PCR amplification). | 2012-08-09 |
20120202204 | Assay for Determining a Molecular Risk Assessment of a Complex Polymicrobial Sample Suspected to Contain an EHEC - The present invention relates to a process to perform a molecular risk assessment (MRA) upon a sample suspected to contain an enterohemorrhagic | 2012-08-09 |
20120202205 | DIAGNOSTIC METHODS - This invention relates to a method of determining the susceptibility of an individual to statin-induced myopathy, comprising detecting the presence or absence of one or more polymorphisms in the SLCO1B1 gene in a biological sample from an individual, whereby the presence of one or more polymorphisms indicates that the individual has altered susceptibility to statin-induced myopathy. | 2012-08-09 |
20120202206 | PHARMACEUTICAL COMPOSITIONS AND METHODS USEFUL FOR MODULATING ANGIOGENESIS, INHIBITING METASTASIS AND TUMOR FIBROSIS, AND ASSESSING THE MALIGNANCY OF COLON CANCER TUMORS - Methods and compositions suitable for modulating angiogenesis in a mammalian tissue are provided. Further provided are methods suitable for inhibiting metastasis and fibrosis in a mammalian tissue and for assessing the malignancy of colon cancer tumors. | 2012-08-09 |
20120202207 | GENETIC ALTERATIONS IN ISOCITRATE DEHYDROGENASE AND OTHER GENES IN MALIGNANT GLIOMA - We found mutations of the R132 residue of isocitrate dehydrogenase 1 (IDH1) in the majority of grade II and III astrocytomas and oligodendrogliomas as well as in glioblastomas that develop from these lower grade lesions. Those tumors without mutations in IDH1 often had mutations at the analogous R172 residue of the closely related IDH2 gene. These findings have important implications for the pathogenesis and diagnosis of malignant gliomas. | 2012-08-09 |
20120202208 | NUCLEIC ACID AND CORRESPONDING PROTEIN ENTITLED 193P1E1B USEFUL IN TREATMENT AND DETECTION OF CANCER - A novel gene 0193P1E1B (also designated 193P1E1B) and its encoded protein, and variants thereof, are described wherein 193P1E1B exhibits tissue specific expression in normal adult tissue, and is aberrantly expressed in the cancers listed in Table I. Consequently, 193P1E1B provides a diagnostic, prognostic, prophylactic and/or therapeutic target for cancer. The 193P1E1B gene or fragment thereof, or its encoded protein, or variants thereof, or a fragment thereof, can be used to elicit a humoral or cellular immune response; antibodies or T cells reactive with 193P1E1B can be used in active or passive immunization. | 2012-08-09 |
20120202209 | CELL IMAGING METHOD FOR VIEWING MICRORNA BIOGENESIS IN THE CELLS - The present invention relates to a method for viewing the biogenesis of at least one microRNA, preferably a microRNA group, in a living cell, characterised in that said method includes the following steps: transforming said cell by an encoding vector for a protein selected among the DGCR8 protein (DiGeorge syndrome critical region gene 8), the Drosha protein and derivatives thereof, said protein being coupled with a marker; expressing said protein coupled with said marker; and detecting said marker. | 2012-08-09 |
20120202210 | Biohazard detection system with exhaust stream recycling - A system capable of detecting low-level releases of bio-agents and other harmful substances contained in air concentrates and recycles exhaust air from a collector in order to entrain and retain more particles from an aerosol release event. The system ensures that particles that were not initially entrained or subsequently retained will have successive opportunities to be entrained within the collection solution. The system also optionally utilizes concentrators in series and a collector to ensure that the particle content of air entering the collector is maximized. | 2012-08-09 |
20120202211 | APPARATUS AND METHOD FOR BIOLOGIC SAMPLE RAPID COLLECTION AND RECOVERY DEVICE, AND CONVENIENT STORAGE - In accordance with exemplary embodiments, apparatus and method for biologic sample rapid collection and recovery device, and convenient storage are provided. An exemplary embodiment includes an apparatus comprising a lateral flow technology device including at least a membrane configured to bind an analyte from a sample that flows through the lateral flow technology device, in which a selected portion of the membrane bound to the analyte when placed directly in an analysis system does not substantially inhibit analysis of the analyte. An elution protocol is not required to extract the analyte bound from the selected portion before placed directly in the analysis system. | 2012-08-09 |
20120202212 | METHODS FOR GENETIC COMPOSITION ANALYSIS OF NATURAL PRODUCTS - Provided herein are novel methods for identifying component species in a natural product using genetic testing methods. | 2012-08-09 |
20120202213 | OLIGONUCLEOTIDES FOR AMPLIFYING CHLAMYDOPHILA PNEUMONIAE NUCLEIC ACID - Amplification oligonucleotides for use in amplifying a sequence contained in nucleic acid derived from | 2012-08-09 |
20120202214 | METHOD FOR DETECTING FUSION GENE - Disclosed is a means which enables simple and rapid detection of the presence of any fusion genes including even unknown fusion genes. The method for measuring a fusion gene(s) according to the present invention is applied to a sample separated from a living body, and comprises: measuring expressions of a 5′-region and a 3′-region of one of the component genes of the fusion gene(s) which may be present in the living body, wherein said 5′-region is upstream of and said 3′-region is downstream of a fusion point in said one of the component genes; and comparing the expression of the 5′-region with the expression of the 3′-region. The fusion gene to be measured is e.g. a fusion gene between ALK gene and another gene. | 2012-08-09 |
20120202215 | MITOCHONDRIAL FUNCTION OF PROHIBITIN 2 (PHB2) - The present invention relates to a PHB2 gene regulator and a therapeutic drug for mitochondrial-function-related disease containing the same, for example. | 2012-08-09 |
20120202216 | NOVEL PHOTOACTIVABLE FLUORESCENT DYES FOR OPTICAL MICROSCOPY AND IMAGING TECHNIQUES - The present invention relates to novel photoactivable rhodamine or carbopyronine derivatives of the following general formulae G1-G4 (I), G1: A1=O, A2=N, A3=C; G2: A1=S, A2=N, A3=C; G3: A1=O, A2=O, A3=N; G4: A1=S, A2=O, A3=N; comprising UV light absorbing chromophores which after photolysis by irradiation at 254-490 nm (preferably at 375-420 nm) generate fluorescent rhodamine or carbopyronine derivatives and small non-toxic fragments such as N | 2012-08-09 |
20120202217 | AUTOANTIBODY ENHANCED IMMUNOASSAYS AND KITS - The present disclosure provides immunoassays and kits for detection or quantification of an analyte of interest in a test sample that potentially contains endogenously produced autoantibodies reactive with the analyte | 2012-08-09 |
20120202218 | DETECTION METHOD AND DEVICE BASED ON NANOPARTICLE AGGREGATION - A method for determining the presence of a compound in a liquid solution, by admixing the liquid solution with a plurality of nanoparticles; providing conditions effective to cause aggregation of the nanoparticles in the liquid solution in the absence of said compound in the liquid solution; and observing a detectable signal reflecting the amount of aggregation of nanoparticles in the liquid solution, wherein the presence of the compound in the liquid solution results in a detectable signal reflecting a reduced amount of aggregation of nanoparticles in the liquid solution, in comparison to the amount of aggregation of nanoparticles obtained in the liquid solution in the absence of the compound therein. A nanoparticle, a composition, a kit and a for multi-well plate for use in the method are also disclosed. In some embodiments the association is cation-, anion and/or PH induced e.g. by using helix-loop-helix polypeptides as first and second molecules attached to the nanoparticles. The first molecules are directed to the target compound, the second molecules allow for aggregation of the nanoparticles. | 2012-08-09 |
20120202219 | PSA CAPTURE AGENTS, COMPOSITIONS, METHODS AND PREPARATION THEREOF - Disclosed herein are novel synthetic prostate specific antigen (PSA)-targeted capture agents that specifically bind PSA. In certain embodiments, these PSA capture agents are biligand or triligand capture agents containing two or three target-binding moieties, respectively. | 2012-08-09 |
20120202220 | MULTIVALENT FLUORESCENT PROBES - Multivalent fluorescent probes and methods of using these multivalent fluorescent probes for in vitro and in vivo imaging are described. | 2012-08-09 |
20120202221 | NATURAL PEPTIDE AND DERIVATIVES AS MODULATORS OF GPCR GPR1 AND USES THEREOF - The invention relates to the identification of Humanin and derivatives thereof as ligands of the GPR1 GPCR (G-protein coupled receptor). The invention encompasses the use of the interaction of GPR1 polypeptides and Humanin polypeptides as the basis of screening assays for agents that modulate the activity of the GPR1 receptor. The invention also encompasses diagnostic assays based upon the GPR1/Humanin polypeptide interaction, as well as kits for performing diagnostic and screening assays. | 2012-08-09 |
20120202222 | ENZYME FRAGMENT COMPLEMENTATION ASSAYS FOR MONITORING THE ACTIVATION OF THE VOLTAGE-GATED POTASSIUM ION CHANNEL HERG - The present invention provides methods and cell based assays for testing for the binding of a ligand to a human Ether-a-go-go-related (hERG) voltage-gated potassium ion channel protein in an enzyme complementation assay. The invention is of particular use in toxicological and drug screening, particularly for high throughput screening. | 2012-08-09 |
20120202223 | Ki-67 ASSAY FOR PATIENT IMMUNE SYSTEM STATUS - Novel diagnostic assay based on the detection of Ki-67 expression to anticipate the response to anti-HIV therapy | 2012-08-09 |
20120202224 | POLYPEPTIDE VARIANTS WITH ALTERED EFFECTOR FUNCTION - The present invention concerns polypeptides comprising a variant Fc region. More particularly, the present invention concerns Fc region-containing polypeptides that have altered effector function as a consequence of one or more amino acid modifications in the Fc region thereof. | 2012-08-09 |
20120202225 | METHODS AND DEVICES FOR IMMUNODIAGNOSTIC APPLICATIONS - Methods and devices for evaluating a sample from a subject for detecting a target red blood cell protein or antibody are disclosed. In one embodiment, optimized antibody screening methods and devices significantly reduce the level of non-specific binding to a surface (e.g., a test surface bound with a red blood cell preparation), thus allowing for more efficient detection and reduced test time. In other embodiments, the invention provides methods and devices for target capturing that include a substantially planar surface, optionally having an optimized angle, for capture. Alternative solid phase geometries for capture are disclosed. Optimized methods for cell deposition are also disclosed. Thus, optimized methods, devices, kits, assays for evaluating a sample are disclosed. | 2012-08-09 |
20120202226 | METHOD AND SYSTEM FOR THE DETECTION OF CANCER - Disclosed are a method and kit of diagnosis of cancer in a body sample of a subject, comprising contacting the sample with at least two different suitable antigens to form at least two different complexes with antibodies present in the sample, determining the actual levels of each of said antigen-antibody complexes in said sample and establishing the ratio between the levels of the different complexes in said subject; and comparing the ratio to a predetermined ratio between antigen-antibody complexes levels formed between the same at least two antigens and samples from healthy subjects, whereby if said ratio determined in step higher or lower than a predetermined cutoff point pre-established for healthy subjects, said subject is diagnosed with cancer. The method and kit can be used for diagnosing various types of cancer, including breast, ovary, lung, prostate and colon cancer. | 2012-08-09 |
20120202227 | Babesia microti Genomic Clones Containing Novel Antigens Useful in the Diagnosis of Babesiosis - Disclosed are the cloning and expression of novel antigens in | 2012-08-09 |
20120202228 | Antibody Profiles Characteristic of Tuberculosis State - Serum antibody assays capable of distinguishing cases of inactive TB from cases of active TB include a combination at least three | 2012-08-09 |
20120202229 | Immunoassay tracer reagents and methods for measuring the amount of native human bioactive hepcidin - The invention provides compositions and methods for measuring human serum hepcidin levels. The invention provides methods for the oxidative refolding of a hepcidin polypeptide to a form that is mature, bioactive and folded as in the native configuration and molecular mass; a method for measuring the level of native, bioactive hepcidin in a vertebrate animal. | 2012-08-09 |
20120202230 | Liquid Reagent of Thyroid Hormone-Immobilized Carrier and Use Thereof - A liquid reagent of a stabilized thyroid hormone-immobilized carrier, by which a thyroid hormone can be measured easily in a short time and at low cost, is provided. The liquid reagent of a thyroid hormone-immobilized carrier according to the present invention includes: a thyroid hormone-immobilized carrier; and a solvent, and a pH of the solvent containing the thyroid hormone-immobilized carrier is in a range from 8.7 to 11.5. The detection of a thyroid hormone using the liquid reagent of the present invention can be carried out by competitively binding a thyroid hormone in a sample and the thyroid hormone-immobilized carrier in the liquid reagent with an anti-thyroid hormone antibody and detecting a composite of the thyroid hormone-immobilized carrier and the anti-thyroid hormone antibody. | 2012-08-09 |
20120202231 | SYNERGISTIC BIOMARKER ASSAY OF NEUROLOGICAL CONDITION USING S-100B - Processes and assays are provided for detecting and determining the magnitude of traumatic brain injury such as that from impact or percussive trauma or stroke. The inventive assays and processes recognize a synergistic correlation between detection of S-IOOb and one or more other injury specific biomarkers. | 2012-08-09 |
20120202232 | Heparin-insensitive Method for Determining Direct Coagulation Factor Inhibitors - The invention is in the field of coagulation diagnostics and relates to a heparin-insensitive method for determining direct coagulation factor inhibitors in a sample, in particular direct thrombin and factor Xa inhibitors. | 2012-08-09 |
20120202233 | Methods and uses of KSR kinase, and mutations thereof - Mutant KSR proteins are disclosed. The mutants include single amino acid substitutions, leading to either a loss of kinase activity or a loss of scaffolding activity. Also disclosed are methods of screening compounds for inhibitors of KSR kinase activity or KSR scaffolding activity. In some embodiments, the screening methods include protein complementation assays in which nucleic acids encoding fusion constructs comprising enzyme portions and kinase dimerization domains are expressed in cells. Inhibitors of dimerization can be indicated by loss of enzyme activity. | 2012-08-09 |
20120202234 | METHODS AND COMPOSITION FOR ISOPRENOID DIPHOSPHATE SYNTHESIS - Provided herein are methods and compositions relating to the synthesis of isoprenoid diphosphates using a mutated isopentenyl phosphate kinase. | 2012-08-09 |
20120202235 | NOVEL FRUCTOSYL PEPTIDYL OXIDASE - In one form, a fructosyl peptidyl oxidase derived from a budding yeast | 2012-08-09 |
20120202236 | COMPOSITE ANIMAL LITTER MATERIAL AND METHODS - A composite litter material for detecting a disease, such as urinary tract disease, when contacted by feline urine includes an absorptive polymer material forming a solid matrix, exfoliated clay embedded and dispersed within the solid matrix, a chromogenic indicator provided within the solid matrix, and an oxidizing agent distributed and stabilized within the solid matrix to be responsive to peroxidase activity in the urine to activate the chromogenic indicator. A method for detecting or diagnosing a feline disease includes contacting urine with the material and detecting a color change. A method of manufacturing the composite material includes preparing a mixture comprising the absorptive polymer and water and distributing the exfoliated clay, chromogenic indicator and oxidizing agent within the mixture, preferably by extrusion and addition of the components at particular points in processing the polymer mixture. | 2012-08-09 |
20120202237 | PARTICLE SORTING APPARATUS AND METHOD - A particle analyzing and/or sorting apparatus and the associated methods. One aspect of the described embodiments relates to an analyzer, or a sorter, having acquisition and sort electronics in the form of a field programmable gate array for processing detected signals. Another aspect relates to a droplet based approach of analyzing and sorting particles and may further include a dynamic element, such a dynamic drop delay. In still another broad aspect, an apparatus and method for dynamically varying other sorting parameters. | 2012-08-09 |
20120202238 | DETERMINING CONDITIONS IN CENTRIFUGED BLOOD USING MEASURED PRESSURE - A method for determining a condition in a blood sample includes: providing a sample of blood; providing a metering probe having a pump for aspirating and dispensing; inserting the metering probe a selected distance into the blood sample; measuring the pressure between the sample and pump during sample aspiration or sample dispense; comparing the measured pressure with a reference value; and signaling the presence or absence of the condition. A method for confirming or detecting the presence of a selected layer of blood component in a centrifuged blood sample includes: measuring a pressure of a suspected selected layer in a metering probe during aspiration or dispense; comparing the measured pressure with a reference value, wherein if the measured pressure and the reference value are substantially identical then the selected layer of the blood component is confirmed. In a preferred embodiment the reference value is a pre-selected pressure range. | 2012-08-09 |
20120202239 | MATERIALS, MONITORING, AND CONTROLLING TISSUE GROWTH USING MAGNETIC NANOPARTICLES - Systems and method for releasing a biological factor in a tissue or organ are disclosed. The system includes one or more nanoparticles distributed in the tissue or organ, the nanoparticles including the biological factor; and a magnetic field generator configured to generate a magnetic field at a first frequency and to apply to the tissue or organ the magnetic field at the first frequency thereby causing at least some of the biological factor to be released from each of the nanoparticles into the tissue or organ. | 2012-08-09 |
20120202240 | Method for Predicting the likelihood of an Onset of an Inflammation Associated Organ Failure - The present invention relates to a reliable and statistically significant method for predicting the likelihood of an onset of an inflammation associated organ failure from a biological sample of a mammalian subject in vitro, by means of a subject's quantitative metabolomics profile comprising a plurality of endogenous metabolites, and comparing it with a quantitative reference metabolomics profile of a plurality of endogenous organ failure predictive target metabolites in order to predict whether the subject is likely or unlikely to develop an organ failure. Furthermore, the invention relates to the usefulness of endogenous organ failure predictive target metabolites in such a method. | 2012-08-09 |
20120202241 | Histological specimen treatment apparatus and method - Method and apparatus for processing tissue specimens against decomposition, putrefaction and autolysis which use a simple three step procedure in a single vessel or container. First, the specimens are saturated with a solvent mixture of a ketone and a hydrocarbon, e.g., an Acetone/Hexane or an Acetone/Xylene mixture, to dissolve lipids and other cellular solutes. The container is then flooded with melted Paraffin. In a last step, the solvent mixture is vaporized and evacuated from the container, allowing the melted Paraffin to replace the vaporized solvent and impregnate the specimens. Raw, i.e., non-processed and non-burred specimens up to 5 mm thick can be processed in about 60 minutes. A solvent regenerator distills the evacuated solvent; and converts vent waste gases into carbon dioxide and water through a thermocatalytic oxidizer. | 2012-08-09 |
20120202242 | System and Method for Non-Sterile Heterotrophic Algae Growth - A system and method for non-sterile heterotrophic algae growth is provided. The system includes a first Continuous Stirred Tank Reactor (CSTR), kept under sterile conditions, for mixing a full load nutrient with algae cells to create a culture. After being transferred to a first Plug Flow Reactor (PFR), a nutrient is depleted from the culture to create an effluent. By depleting the nutrient, algae cells are able to grow more rapidly without having to compete with other microbes for available nutrients. Once the effluent is created, it is transferred to a second PFR where organic carbon is added. By adding organic carbon, the algae cells grow intracellular oil rapidly. Algae cells are then removed for processing into biofuel. | 2012-08-09 |
20120202243 | IN Vivo Incorporation of Unnatural Amino Acids - The invention provides methods and compositions for in vivo incorporation of unnatural amino acids. Also provided are compositions including proteins with unnatural amino acids. | 2012-08-09 |
20120202244 | Antibodies Capable of Specifically Binding to a Specific Amino Acid Sequence - The present invention provides for an antibody or fragment thereof capable of specifically binding to an epitope of the amino acid sequence CDPAFLYKVVD (SEQ ID NO:1) or a fragment of at least 5, 6, or 7 amino acids thereof. | 2012-08-09 |
20120202245 | Method for Producing Natively Folded Proteins in a Prokaryotic Host - The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds. | 2012-08-09 |
20120202246 | Vector Comprising Mannose Promoter and Mannose Promoter - The present invention relates to a vector expressible in a prokaryotic host and a nucleic acid sequence comprising a mannose-inducible promoter of the mannose operon of | 2012-08-09 |
20120202247 | METHOD, COMPUTING ROUTINE, DEVICE FOR PREDICTING PROPERTIES OF MHC/PEPTIDE COMPLEXES, AND DATA AND PEPTIDES PRODUCED THEREFROM - The present invention relates to a method for structure-based prediction of properties of peptides and peptide analogs in complex with major histocompatibility (MHC) class I and class II molecules. The said properties mainly relate to the three-dimensional structure of an MHC/peptide complex and the binding affinity of a peptide for an MHC receptor. The invention further relates to a computer program and a device therefor. The invention further relates to data produced by a method of the invention. The invention further relates to peptides and peptide analogs predicted to bind to target-MHC molecules. The present invention thus relates to the field of immunology, with possible applications in manufacture of vaccinates, de-immunization of proteins, and manufacture of therapeutic agents, especially immuno-therapeutic agents. | 2012-08-09 |
20120202248 | NOVEL HANSENULA POLYMORPHA GENE CODING FOR DOLICHYL-PHOSPHATE- MANNOSE DEPENDENT ALPHA-1,3 MANNOSYLTRANSFERASE AND PROCESS FOR THE PRODUCTION OF RECOMBINANT GLYCOPROTEINS WITH HANSENULA POLYMORPHA MUTANT STRAIN DEFICIENT IN THE SAME GENE - The present invention relates to a process for producing a human-type glycoprotein having reduced glycosylation by genetically manipulating an enzyme involved in glycosylation using a | 2012-08-09 |
20120202249 | PRODUCTION OF AN INTACT VIRUS IN A MAMMALIAN (NON-HOST) CELL SYSTEM USING A SECONDARY NON-HOST VIRAL CONSTRUCT - The present invention relates to constructs and methods for the production of recombinant proteins, viruses, and viral vaccines in heterologous culture systems by expressing intact genes or viral genomes under the control of a pantropic promoter in a culture system that is not considered a host to the virus so produced. The promoter/viral genome constructs are inserted into a baculovirus and expressed in mammalian non-host cells for the baculovirus. | 2012-08-09 |
20120202250 | Method for Exponential Amplification of RNA Using Thermostable RNA-dependent RNA Polymerase - The present invention relates to a method for exponential amplification of RNA in vitro by using a thermostable RNA-dependent RNA polymerase (RdRp) of a | 2012-08-09 |
20120202251 | IN VIVO ASSEMBLY OF DNA VIA HOMOLOGOUS RECOMBINATION - According to the present invention, a DNA construct of interest is assembled from overlapping subfragments via an acceptor module which comprises the distal end of the construct at a position downstream from a promoter. The construct is assembled distal to proximal via homologous recombination events occurring in the span between that distal end of the construct and the upstream end of the promoter. These recombination events occur iteratively between the acceptor module and alternative donor modules. Successful recombination places one of at least two marker genes under the transcriptional control of an active form of the promoter. As a result of alternating use of two varieties of donor modules, as few as two selection markers may be used to produce a complex DNA construct. | 2012-08-09 |
20120202252 | EFFECT OF RADIATION ON CELLULASE ENZYMES - A method for recycling cellulase enzymes. Also provided is a method for producing fermentable carbohydrates, plant leaf protein, and lignin, by adding a cellulase enzyme complex expressed from and on irradiated cellulase complex-producing organisms with sufficient radiation to kill biological activity without destroying all cellulase enzyme complex activity to biomass. The fermentable carbohydrates produced by the method. Also provided are irradiated cellulase-producing organisms for use in converting biomass to fermentable sugars, plant leaf protein, and lignin. A method for producing cellulase enzymes for glucose and other sugar production and protein and lignin extraction by irradiating cellulase-producing organisms, thereby producing the cellulase enzymes is also provided. A system for producing fermentable carbohydrates, plant protein, and lignin, said system comprising irradiated cellulase-producing organisms and biomass is provided. | 2012-08-09 |
20120202253 | ALCOHOL SULFITE BIOREFINERY PROCESS - A biorefinery process to fractionate lignocellulosic materials into cellulose, hemicelluloses and lignin using a pretreatment with mixture of alcohol, sulfur dioxide and water. Further treatment with enzymes, micro-organisms, and optionally bisulfite ion, are used to convert intermediate products to alcohol and lignin derivatives. | 2012-08-09 |
20120202254 | METHOD FOR PRODUCING LACTIC ACID BY NON-STERILE FERMENTATION - The present invention relates to a method for producing lactic acid from plant biomass without requiring sterilization, and specifically relates to a method for producing lactic acid comprising culturing alkaliphilic lactic acid bacteria under non-sterile condition and at a pH of 9 to 11 in a medium containing a cellulose glycosylation solution and then further culturing the bacteria at a pH of 5 to 9. | 2012-08-09 |
20120202255 | METHOD FOR PRODUCING AN L-AMINO ACID - A method for producing an L-amino acid by preparing a processed product of a microalgae, which promotes production and accumulation of the L-amino acid by a bacterium having an ability to produce the L-amino acid, by culturing the microalgae in a medium, and processing the resulting culture at a midtemperature; culturing the bacterium in a medium containing the processed product of the microalgae to produce and accumulate the L-amino acid in culture; and collecting the L-amino acid from the culture. | 2012-08-09 |
20120202256 | NOVEL PREPARATION METHOD OF HUMAN METABOLITES OF SIMVASTATIN OR LOVASTATIN USING BACTERIAL CYTOCHROME P450 AND COMPOSITION THEREFOR - A method of producing human metabolites of simvastatin or lovastatin, and more particularly, a method of producing human metabolites of simvastatin and lovastatin by using bacterial cytochrome P450 BM3(CYP102A1) or mutants thereof, and a composition and kit therefor. | 2012-08-09 |
20120202257 | LIGE-TYPE ENZYMES FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 2012-08-09 |
20120202258 | LIGE-TYPE TRANSFORMANTS FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 2012-08-09 |
20120202259 | METABOLIC EVOLUTION OF ESCHERICHIS COLI STRAINS THAT PRODUCE ORGANIC ACIDS - This invention relates to the metabolic evolution of a microbial organism previously optimized for producing an organic acid in commercially significant quantities under fermentative conditions using a hexose sugar as sole source of carbon in a minimal mineral medium. As a result of this metabolic evolution, the microbial organism acquires the ability to use pentose sugars derived from cellulosic materials for its growth while retaining the original growth kinetics, the rate of organic acid production and the ability to use hexose sugars as a source of carbon. This invention also discloses the genetic change in the microorganism that confers the ability to use both the hexose and pentose sugars simultaneously in the production of commercially significant quantities of organic acids. | 2012-08-09 |
20120202260 | HYBRID BIOREFINING AND GASIFICATION OF LIGNOCELLULOSIC FEEDSTOCKS - Processes and systems for concurrent recovery of lignin derivatives and syngas from a lignocellulosic feedstock. The processes and systems therefor generally comprise the steps of: (a) perfusing and cooking the lignocellulosic feedstock with a suitable organic solvent for a suitable period of time thereby producing a cellulosic solids output stream and a spent liquid solvent stream, said spent liquid solvent stream comprising solubilized lignin derivatives and other organic compounds; (b) separating said cellulosic solids output stream and said spent liquid solvent stream; (c) recovering lignin derivatives from the spent liquid solvent stream thereby producing at least a partially de-lignified spent liquid solvent stream; (d) recovering a portion of the organic solvent from the at least partially de-lignified spent liquid solvent stream thereby producing a stillage; and (e) gasifying the cellulosic solids output stream thereby producing a combustible syngas. | 2012-08-09 |
20120202261 | 3D TROPHOBLAST MATRIX FOR PREPARING ORGAN-SPECIFIC STEM CELLS - This disclosure relates generally to methods, apparatuses, and cellular compositions and cellular products that use a unique trophoblast-containing 3D matrix as a compartmental chamber for growing embryoid bodies that can be induced to differentiate into organ-specific cell types. | 2012-08-09 |
20120202262 | PROCESS FOR PRODUCTION OF AN ENZYME PRODUCT - The invention relates to a process for production of an enzyme product having a plurality of enzyme activities obtained by fermentation of an | 2012-08-09 |
20120202263 | Bioactive Macromers and Hydrogels and Methods for Producing Same - The invention concerns macromers, having a molecular weight of at least 2 kDa, comprising at least one unit of the formula P-(protein-P) | 2012-08-09 |
20120202264 | INHIBITORS OF IL2-INDUCIBLE T-CELL KINASE - Described herein are irreversible kinase inhibitor compounds, methods for synthesizing such irreversible inhibitors, and methods for using such irreversible inhibitors in the treatment of diseases. Further described herein are methods, assays and systems for determining an appropriate irreversible inhibitor of a protein, including a kinase. | 2012-08-09 |
20120202265 | METHODS FOR REDUCING BLUE SACCHARIDE - The present disclosure relates to a | 2012-08-09 |
20120202266 | POLYPEPTIDES HAVING ENDOGLUCANASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to isolated polypeptides having endoglucanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides. | 2012-08-09 |
20120202267 | METHOD FOR THE PURIFICATION OF ADENOVIRUS PARTICLES - The invention provides methods for large-scale adenovirus purification from high cell density suspensions, using host cell DNA precipitation followed by a clarification step. | 2012-08-09 |
20120202268 | PROCESS FOR ADENOVIRUS PURIFICATION FROM HIGH CELL DENSITY CULTURES - The invention provides methods for large-scale adenovirus purification from high cell density suspensions, using host cell DNA fragmentation and/or precipitation followed by a clarification step with tangential flow filtration. | 2012-08-09 |
20120202269 | GELATINOUS SUBSTANCE BASED GROWTH MEDIUM FILTRATION DEVICE AND METHOD - Growth medium filtration devices are provided which are particularly well adapted to sterilize gelatinous substance based growth mediums including agar, agarose and other polysaccharide derived growth mediums for use in agar plates and the like for culturing microorganisms. The devices may include one or more filtration units and at least one heat source for filtering the growth medium in a heated filtration process. Methods of filtering a growth medium, such as an agar growth medium, and methods of preparing a growth medium having low gelling point and lower molecular weight characteristics are also provided. | 2012-08-09 |
20120202270 | Producing an immune response for reducing the risk of developing brucellosis - This invention relates to unique antigens in a brucellosis vaccine which Provide an immune response against bacteria which cause brucellosis in cattle and other animals. The vaccine specifically uses L7L12, 85A, Bp26 and/or LeuB and may or may not include GFP with any combination of polypeptides | 2012-08-09 |
20120202271 | Method producing an immune response for reducing the risk of developing brucellosis - This invention relates to unique antigens in a brucellosis vaccine which Provide an immune response against bacteria which cause brucellosis in cattle and Other animals. The vaccine specifically uses Bp26 and LeuB expressed to create the immunity. | 2012-08-09 |
20120202272 | LIGE-TYPE SYSTEMS FOR BIOCONVERSION OF LIGNIN-DERIVED COMPOUNDS - The teachings provided herein are generally directed to a method of converting lignin-derived compounds to valuable aromatic chemicals using an enzymatic, bioconversion process. The teachings provide a selection of (i) host cells that are tolerant to the toxic compounds present in lignin fractions; (ii) polypeptides that can be used as enzymes in the bioconversion of the lignin fractions to the aromatic chemical products; (iii) polynucleotides that can be used to transform the host cells to express the selection of polypeptides as enzymes in the bioconversion of the lignin fractions; and (iv) the transformants that express the enzymes. | 2012-08-09 |
20120202273 | Enzymatic Conversion Of Blood Group A, B, And AB Red Blood Cells Using Alpha-N-Acetylgalactosaminidases and Alpha-Galactosidases With Unique Substrate Specificities And Kinetic Properties - This invention relates to enzymatic removal of type A and B antigens from blood group A, B, and AB reactive cells in blood products, and thereby converting these to non-A and non-B reactive cells. The invention further relates to using unique α-N-acetylgalactosaminidases and α-galactosidases with superior kinetic properties for removing the immunodominant monosaccharides of the blood group A and B antigens and improved performance in enzymatic conversion of red blood cells. The preferred unique α-N-acetylgalactosaminidases and α-galactosidases exhibit the following characteristics: (i) exclusive, preferred or no less than 10% substrate specificity for the type A and B branched polysaccharide structures relative to measurable activity with simple mono- and disaccharide structures and aglycon derivatives hereof; (ii) optimal performance at neutral pH with blood group oligosaccharides and in enzymatic conversion of cells; and (iii) a favorable kinetic constant K | 2012-08-09 |
20120202274 | Systems and Methods for Cultivating, Harvesting and Processing Biomass - Combining controlled open-ocean iron enrichment with a system for collecting the ensuing biological growth can lead to a fundamental shift towards using marine biomass feedstock for large-scale global biodiesel production. The literature review reveals that open-ocean enrichment effectively reduces both the atmospheric carbon dioxide partial pressure and ocean acidity. A semi-closed ocean system is provided that allows for the efficient cultivation and harvesting of a high tonnage biomass feedstock generated by iron fertilization. The concept methodically capitalizes on the ocean's free nutrients, kinetic/potential energy, and expansive surface area to ensure that the mass, energy, and cost balance equations favor our system while taking care to preserve the ocean's ecosystem. The system is modular, portable, easily scalable system, and minimizes waste. In addition to the above benefits, our concept allows continued adherence to the NEPA and London Protocol by culling the biomass produced by fixing carbon dioxide and limiting iron exposure to the vessel's interior. | 2012-08-09 |
20120202275 | SYSTEM AND METHOD FOR REMOVING AIRBORNE CONTAMINANTS FROM EXHAUST GAS - The present invention provides a system and method for purifying and/or filtering gases. The system and method remove undesired species from a gas through the use of bioorganisms which reside in and on a filtration media. Water and nutrients can be supplied to the filtration media to promote the growth and biological activity of the bioorganisms. | 2012-08-09 |
20120202276 | Modified Proteins and Methods of Making and Using Same - Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions. | 2012-08-09 |
20120202277 | MINIMALLY INVASIVE CYTOMETRY SYSTEM WITH QCL INSPECTION OF SINGLE CELLS FOR CANCER DETECTION - This disclosure concerns a minimally invasive cytometry system including a handling system that presents single cells to at least one QCL laser source. The QCL laser source is configured to deliver light to a cell within the cells in order to induce vibrational bond absorption in one or more analytes within the cell. The cytometry system also includes a detection facility that detects the mid-infrared wavelength light transmitted by the cell and identifies the cell as either cancerous or non-cancerous. | 2012-08-09 |
20120202278 | SYSTEM AND METHOD FOR REPEAT MEASUREMENT OF SINGLE CELLS USING VIBRATIONAL SPECTROSCOPY - This disclosure concerns a high-throughput cytometry system including a handling system that presents single cells to at least one quantum cascade laser (QCL) source. The QCL laser source is configured to deliver light successively to single cells over a time period in order to make successive measurements of induced resonant mid-infrared absorption by one or more analytes of the cells. The cytometry system also includes a mid-infrared detection facility that detects the successively transmitted mid-infrared wavelength light, wherein the transmitted mid-infrared wavelength light is used to identify a changing cell characteristic over the time period. | 2012-08-09 |
20120202279 | ONSITE INTEGRATED PRODUCTION FACTORY - An onsite integrated production factory having an electrolytic plant, an ethanol plant, a vegetable plant, a culturing plant, and a power generation unit. The onsite integrated production facility is arranged so that fresh water, sulfuric acid or caustic soda produced by and received from the electrolytic plant is used in the ethanol plant to produce and output oxalic acid, sodium oxalate, fuel bioethanol. The oxalic acid or sodium oxalate produced in and received from the ethanol plant is used for removing calcium contained in the sea water in the electrolytic plant. The vegetable plant produces vegetables for generation and outputting of oxygen by receiving the carbon dioxide generated during the fermentation process in the ethanol plant and fresh water from the electrolytic plant. The culturing plant has a fishery farm or reef for using the oxygen generated in the vegetable plant. | 2012-08-09 |
20120202280 | TREATMENT SYSTEM OF WET ORGANIC WASTE - Provided is a treatment system whereby a wet organic waste can be effectively treated and CO | 2012-08-09 |
20120202281 | LIGHT ENERGY SUPPLY FOR PHOTOBIOREACTOR SYSTEM - Photobioreactor systems including a supply material processing sub-system, a reactor sub-system, a product material processing sub-system, and a solar energy supply sub-system are provided. The reactor sub-system includes a photobioreactor configured for containing a reaction mixture that is operative for effecting photosynthesis upon exposure to photosynthetically active light radiation. The supply material processing sub-system is configured for supplying the reactor with supply material. The product material processing sub-system is configured for receiving reaction zone product discharged from the reactor and effecting separation of a liquid component from the received reaction zone product. The solar energy supply sub-system includes at least one solar collector mounted to the photobioreactor and includes a solar collector surface configured for receiving incident solar radiation and configured for transmitting energy derived from received incident solar radiation and supplying energy to at least one of the other sub-systems. | 2012-08-09 |
20120202282 | GRAVITY FLOW TUBULAR PHOTOBIOREACTOR AND PHOTOBIOREACTOR FARM - A gravity flow photobioreactor core ( | 2012-08-09 |
20120202283 | FREEZE-DRIED PRODUCT FOR INTRODUCING NUCLEIC ACID, OLIGONUCLEIC ACID OR DERIVATIVE THEREOF - A freeze-dried product of a complex containing (i) a nucleic acid, an oligonucleic acid or a derivative thereof, (ii) polyethyleneimine and (iii) hyaluronic acid or chondroitin sulfate. The freeze-dried product can be used to introduce a nucleic acid or an oligonucleotide into a cell. | 2012-08-09 |
20120202284 | CELL SORTING APPARATUS AND METHOD - Apparatus and method for sorting cells is provided in which two or more cells are delivered along a flow path, the two or more cells including a first type of cells having a first mechanical stiffness and at least a second type of cells having a second mechanical stiffness different from the first mechanical stiffness. A reflection surface is provided across the flow path at an oblique angle relative to the flow path and configured to reflect the cells at different reflection angles relative to the flow path dependent on the mechanical stiffness of the cells. | 2012-08-09 |
20120202285 | ANTIBODIES TO IL-6 AND THEIR USES - Antibodies and antigen-binding portions thereof that bind to human IL-6 are provided. Also provided are nucleic acids encoding such antibodies and antigen binding portions, methods of making such antibodies and antigen binding portions, compositions comprising such antibodies or antigen binding portions, and uses of such antibodies or antigen binding portions. | 2012-08-09 |
20120202286 | rbLIF PROTEIN FOR USE IN EMBRYONIC STEM CELL CULTURES - Provided herein methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell on a rabbit feeder cell layer, wherein the rabbit feeder layer expresses a leukemia inhibitory factor (LIF) protein. Also provided are methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell in a culture medium comprising rabbit leukemia inhibitory factor (rbLIF) protein. Also provided are polynucleotide and amino acid sequences of a rabbit leukemia inhibitory factor protein (rbLIF). | 2012-08-09 |
20120202287 | Stem Cell Culture Methods - The invention provides methods for reversibly inhibiting stem cell differentiation wherein a compound of formula (I) is contacted with a stem cell. The invention further provides a method for preparing a culture medium, a culture medium supplement and a composition comprising a compound of formula (I). | 2012-08-09 |
20120202288 | COMPOSITIONS COMPRISING CYCLIC AMP ENHANCERS AND/OR EP LIGANDS, AND METHODS OF PREPARING AND USING THE SAME - Provided are improved pharmaceutical compositions that comprise ligands or agonists to prostaglandin EP receptors, and/or cyclic AMP enhancers, and suitable organic solvents that are substantially free of methyl acetate, the compositions being provided for storage and/or use in an endotoxin-free vessel, such as a tube or PE bag. The compositions are suitable for in vitro, ex vivo, and in vivo use, and in particular for ex vivo therapeutic use, such as in hematopoietic stem cell transplants. Also provided are methods of using the compositions in ex vivo therapeutic applications, and methods of preparing the compositions. Kits with instructions on use are also provided. | 2012-08-09 |
20120202289 | SEPARATOR DEVICE, DEPOSITION DEVICE AND SYSTEM FOR HANDLING OF SOMATIC PLANT EMBRYOS - Methods and devices for separating fluid-suspended plant somatic embryos and embryogenic tissue based on differences in their fluid drag properties are disclosed. Deposition method and device for depositing plant somatic embryos into embryo receiver comprising growth substrate by means of a fluid jet is disclosed. An automated system for processing plant somatic embryos from the bioreactor to the growth substrate is also disclosed. | 2012-08-09 |
20120202290 | TUBULAR PHOTOBIOREACTOR - The invention relates to a tubular photobioreactor that has a core structure in the shape of a truncated cone and one or more transparent or translucent tubes which are helically wound around the outer surface and/or inner surface of the core structure. Said tubular photobioreactor is characterized in that the transparent or translucent tube has at least two chambers, through at least one of which the cultivation medium flows and through at least one of which a heat transfer medium flows. | 2012-08-09 |
20120202291 | SIMPLIFIED BASIC MEDIA FOR HUMAN PLURIPOTENT CELL CULTURE - Fully defined media that support pluripotent cell viability, proliferation, cloning, and derivation, as well as methods and compositions including these media are described. Methods for deriving iPS cells from adult individuals under defined, xeno-free conditions are also described. | 2012-08-09 |
20120202292 | Novel method to generate nutritional compounds from microalgae - The present invention relates to the application of genomics technology to generate nutritional compounds in algae, such as microalgae. The present invention discloses a novel method to modify algae to produce a variety of nutritional compounds. Specifically, this method utilizes microalgae genomics technology to manipulate the existing metabolic pathways in the genomes of microalgae and therefore induce the algae to modify secondary metabolic pathways to increase the level of existing nutritional compounds or produce novel nutritional compounds with a variety of commercial applications for improving human and animal nutrition and health. | 2012-08-09 |
20120202293 | Highly Multiplexed Particle-Based Assays - Methods are provided for detecting and optionally quantitating multiple analytes, including nucleic acid and/or polypeptide analytes, in particle-based assays that can be highly multiplexed. Compositions, systems, and kits related to the methods are also featured. | 2012-08-09 |
20120202294 | SYSTEMS AND METHODS FOR WAVELENGTH SPECTRUM ANALYSIS FOR DETECTION OF VARIOUS GASES USING A TREATED TAPE - An apparatus including a treated tape, an adjustable color source, a photodiode, and a processor is provided. The adjustable color source emits a first radiation toward the treated tape and operates at each of a plurality of different target wavelengths in a spectrum. The photodiode measures a second radiation reflected from the treated tape, and the processor analyzes measurements from the photodiode to determine a peak wavelength from the plurality of target wavelengths. Based on the peak wavelength, the processor determines a color and darkness of a color stain on the treated tape. Based on the determined color and darkness of the color stain, the processor also determines a type and concentration of gas to which the treated tape is exposed. | 2012-08-09 |
20120202295 | MATERIALS AND METHODS FOR ASSAY OF ANTI-HEPATITIS C VIRUS (HCV) ANTIBODIES - A polypeptide comprising the contiguous amino acids 1-198 of SEQ ID NO: 2; a polypeptide, which comprises a contiguous amino acid sequence that is at least about 95% identical to the contiguous amino acids 1-198 of SEQ ID NO: 2, an epitope that is immunoreactive with an antibody that specifically binds to the core protein of hepatitis C virus (HCV), and an epitope that is immunoreactive with an antibody that specifically binds to the NS4 region of HCV; a nucleic acid encoding such a polypeptide; a host cell comprising such a nucleic acid; an immunodiagnostic reagent comprising such a polypeptide; a kit comprising such an immunodiagnostic reagent; and a method of determining the presence, amount, or concentration of anti-HCV antibodies in a test sample. | 2012-08-09 |
20120202296 | SYSTEM, METHOD, AND APPARATUS FOR MEASURING AFFINITY CONSTANTS - Nonlinear spectroscopic systems, methods, and apparatuses for measuring affinity constants of molecule-molecule reactions and/or for determining a maximum number of molecule-molecule complexes. Second harmonic generation (“SHG”) or sum-frequency generation is used to probe a sample. A magnitude of a net electric charge at a solid/water interface can be detected and used to determine characteristics of the molecule-molecule complex. | 2012-08-09 |