31st week of 2014 patent applcation highlights part 45 |
Patent application number | Title | Published |
20140212878 | Mutation Detection Assay - A method of sample analysis is provided. In certain embodiments, the method involves: a) amplifying a product from a sample that comprises both wild type copies of a genomic locus and mutant copies of the genomic locus that have a point mutation relative to said wild type copies of the genomic locus, to produce an amplified sample, where: i. the amplifying is done using a first primer and a second primer; and ii. the first primer comprises a 3′ terminal nucleotide that base pairs with the point mutation and also comprises a nucleotide sequence that is fully complementary to a sequence in the locus with the exception of a single base mismatch within 6 bases of the 3′ terminal nucleotide; and b) detecting the presence of said product in said amplified sample using a flap assay that employs an invasive oligonucleotide. A kit for performing the method is also provided. | 2014-07-31 |
20140212879 | METHOD FOR DETECTING TOXIN-PRODUCING CLOSTRIDIUM DIFFICILE - Provided are an oligonucleotide which realizes specific and highly sensitive detection of toxigenic | 2014-07-31 |
20140212880 | SOLID MEDIUM FOR THE STORAGE OF BIOLOGICAL MATERIAL - This invention relates to flat solid media for the storage of samples of biological materials and methods of analysing biomolecules contained within the samples following storage. In particular, the invention relates to the storage and further analysis of biomolecules present in the biological materials, such as proteins, enzymes and nucleic acids. The invention finds particular utility in the dry, room temperature storage of biological materials. | 2014-07-31 |
20140212881 | SYSTEM AND METHOD FOR CAPTURING AND ANALYZING CELLS - A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells. | 2014-07-31 |
20140212882 | PROCESSING PARTICLE-CONTAINING SAMPLES - A microfluidic device includes an input port for inputting a particle-containing liquidic samples into the device, a retention member, and a pressure actuator. The retention member is in communication with the input port and is configured to spatially separate particles of the particle-containing liquidic sample from a first portion of the liquid of the particle containing fluidic sample. The pressure actuator recombines at least some of the separated particles with a subset of the first portion of the liquid separated from the particles. The device can also include a lysing chamber that receives the particles and liquid from the retention member. The lysing chamber thermally lyses the particles to release contents thereof. | 2014-07-31 |
20140212883 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 2014-07-31 |
20140212884 | COMPOSITION AND METHODS FOR RT-PCR COMPRISING AN ANIONIC POLYMER - The present invention is in the fields of molecular biology. The present invention is directed to novel compositions, methods and kits useful for the generation of nucleic acids from an RNA template and further nucleic acid replication. Specifically, the invention is directed to the generation and amplification of nucleic acids by reverse transcriptase-polymerase chain reaction. | 2014-07-31 |
20140212885 | ENDOGENOUS DNASE ACTIVITY TO REDUCE DNA CONTENT - The application provides a method of reducing the DNA content of a protein preparation or a culture broth from a filamentous fungal host cell using an endogenous filamentous fungal host DNase activity. | 2014-07-31 |
20140212886 | SHUTTLE VECTORS FOR CORYNEBACTERIUM AND ESCHERICHIA COLI - A vector that allows for easy accomplishment of a variety of cloning, and use of the vector for measurement of the transcription-inducing activity of a promoter or production of a desired gene product in | 2014-07-31 |
20140212887 | SCREENING METHOD FOR THERAPEUTIC AGENT FOR CHONDROPATHY AND MODIFIED CHONDROCYTE FOR TREATMENT OF CHONDROPATHY - The use of salt-inducible kinase 3 allows screening for a substance having an effect of increasing cartilage volume and/or an effect of normalizing chondrocyte differentiation. A modified chondrocyte in which the expression or function of salt-inducible kinase 3 gene is inhibited or enhanced can be used to ameliorate a disease associated with reduced cartilage volume and/or a disease associated with impaired cartilage growth. | 2014-07-31 |
20140212888 | T CELL RECEPTOR AND USES THEREOF - A method has been developed to efficiently proliferate and culture a CTL specific to WT1 peptides under limiting dilution conditions. Utilizing this method, CTLs capable of recognizing both a state where a wildtype WT1 specific peptide is presented by HLA-A*24:02 and a state where a mutant WT1 specific peptide is presented by HLA-A*24:02 have been successfully obtained. | 2014-07-31 |
20140212889 | METHOD FOR STAINING TISSUE - It is an object of the present invention to provide a tissue staining method that makes it possible to observe both information on the morphology of a tissue and information on a biological substance such as an antigen molecule to be detected on a single section and in a single view field. The present invention provides a tissue staining method, including carrying out (A) a HE (hematoxylin-eosin) staining, and (B) a histochemical staining, serially on a single tissue section, wherein the histochemical staining is defined as a histochemical technique for detecting a biological substance to be detected in a tissue in a visible manner by use of a binding reaction between the biological substance to be detected and a probe biological substance capable of binding specifically to the biological substance to be detected. | 2014-07-31 |
20140212890 | METHODS, APPARATUSES, AND SYSTEMS FOR DETECTING AND QUANTIFYING PHOSPHOPROTEINS - Embodiments herein provide methods, apparatuses, and systems for detecting, monitoring, measuring, and/or characterizing the activity of phosphoproteins, such as tyrosine kinases (TKs) and downstream proteins in TK signal transduction pathways (e.g., TK pathway proteins). In various embodiments, the methods, apparatuses, and systems may use nanoparticles, such as quantum dots (QD), to detect and/or characterize the abnormally overactive TK signaling pathways that underlie tumorgenesis and tumor progression. In various embodiments, the QD-based methods, apparatuses, and systems may have a sufficiently high degree of sensitivity to enable the identification of new TK signaling pathway markers, for example for use in diagnosing, staging, monitoring, and/or prognosing cancers, or in evaluating the efficacy of cancer therapeutics. | 2014-07-31 |
20140212891 | Compositions and Methods Using NOX5 - Certain embodiments include methods of assessing male fertility. | 2014-07-31 |
20140212892 | Method For Automated Tissue Analysis - The invention provides an improved method for identifying and interpreting tissue specimens and/or cells derived from tissue specimens. A panel of cell-based reagents provides a number of readouts of cellular states or biomarkers that together define a profile of a diversity of cellular states or biomarkers in a tissue specimen representing the ‘systems” nature of biology. This cellular profile is interpreted using informatics tools, to identify similarities between specimens, in vivo medical conditions, and suggest options for treating medical conditions. | 2014-07-31 |
20140212893 | L-SERINE COMPOSITIONS, METHODS AND USES FOR TREATING NEURODEGENERATIVE DISEASES AND DISORDERS - L-serine, L-serine precursors, L-serine derivatives and L-serine conjugates for treatment, amelioration and/or prevention of protein aggregation/tangles/plaques and diseases associated with protein aggregation/tangles/plaques. In particular, treatments and uses for L-serine, L-serine precursors, L-serine derivatives and L-serine conjugates include Alzheimer's disease (AD), Parkinson's disease, Amyotrophic Lateral Sclerosis (ALS), and Huntington disease (HD). | 2014-07-31 |
20140212894 | METHOD OF DIAGNOSING SURGICAL SITE INFECTIONS - A marker useful in diagnosing surgical site infections is provided. In the method of the present invention for detecting surgical site infections, sCD14-ST in a sample is measured. | 2014-07-31 |
20140212895 | Cancer Analysis System - The invention provides for detecting target subpopulations of cells that have high proliferative and renewal properties in animals, including circulating tumor cells, cancer stem cells, hematopoietic stem cells and endothelial progenitor cells. The invention utilizes a defined substrate and media of known property to enrich target cell subpopulations which can be used for future genetic, proteomic and morphological analyses. The method can use image-capture and analysis software to characterize cells based on physical properties, such as size, morphology and kinetic properties. | 2014-07-31 |
20140212896 | METHODS AND COMPOSITIONS FOR SINGLE CHAIN VARIABLE REGION ENOX2 ANTIBODIES FOR CANCER DETECTION AND DIAGNOSIS - All neoplastic cells express one or more members of a unique family of cell surface ubiquinone (NADH) oxidase proteins with protein disulfide-thiol interchange activity (ECTO-NOX proteins) that are characteristically inhibited by quinone site inhibitors with anti-cancer activity and a common amino acid sequence disclosed herein that allows for cancer-specific antibody recognition as well as for detection of certain critical reference proteins that provide loading controls. Cancers of different cellular or tissue origins express different ENOX2 cancer isoforms or combinations of isoforms and shed these proteins into the circulation. Herein are disclosed methods both for cancer detection and diagnosis of particular origin, based on the patterns and molecular weights of the isoforms which allow the identification of the cell type and or tissue of origin of the neoplasm. Relative ENOX2 amounts are proportional to tumor burden and provide a reliable measure of response to therapy and disease progression. Also provided is the amino acid sequence to which the scFv antibodies bind as the molecular basis for the specificity of the test. | 2014-07-31 |
20140212897 | METHODS FOR PREDICTING PREGNANCY OUTCOME IN A SUBJECT BY HCG ASSAY - The present invention provides a method of predicting pregnancy outcome in a subject by determining the amount of an early pregnancy associated molecular isoform of hCG in a sample. The present invention further provides a method for determining the amount of early pregnancy associated molecular isoforms of human chorionic gonadotropin (hCG) in a sample. The present invention also provides a diagnostic kit for determining the amount of early pregnancy associated hCG in a sample. The present invention additionally provides an antibody which specifically binds to an early pregnancy associated molecular isoform of human chorionic gonadotropin. Finally, the present invention provides methods for detecting trophoblast or non-trophoblast malignancy in a sample. | 2014-07-31 |
20140212898 | PEPTIDES, DEVICES, AND METHODS FOR THE DETECTION OF EHRLICHIA ANTIBODIES - The invention provides peptide compositions and mixtures useful for the detection of antibodies that bind to | 2014-07-31 |
20140212899 | METHOD FOR NONINVASIVE PREDICTION OR DIAGNOSIS OF INFLAMMATION AND INFECTION IN AMNIOTIC FLUID OF PATIENTS WITH PREMATURE RUPTURE OF MEMBRANES - Provided is a method for noninvasive prediction or diagnosis of inflammation and/or infection in amniotic fluid leaked through the cervix into the vagina to predict the concentration of inflammatory markers in the amniotic fluid inside uterus by measuring the concentration of inflammatory markers (various cytokines). Further provided is a method for prediction or diagnosis of inflammation and/or infection in amniotic fluid by measuring the concentration of markers (IL-6, IL-1β IL-8, MCP-1, GRO-α) in the amniotic fluid leaked through the cervix into the vagina in patients with premature rupture of membranes. The method can be performed more stably on pregnant women, as compared to the conventional method for prediction or diagnosis of inflammation and/or infection using invasively collected amniotic fluid. | 2014-07-31 |
20140212901 | NMR METHODS FOR MONITORING BLOOD CLOT FORMATION - The invention features a method of monitoring a clotting process by measuring a signal characteristic of the NMR relaxation of water in a sample undergoing clotting to produce NMR relaxation data and determining from the NMR relaxation data a magnetic resonance parameter of water in the sample characteristic of the clots being formed. | 2014-07-31 |
20140212902 | HEAT-TREATED LIMULUS AMEBOCYTE LYSATES - The application provides heat-treated | 2014-07-31 |
20140212903 | AZO MEDIATORS AND METHODS OF USE THEREOF - Azo compounds are disclosed that do not form azoxy dimers and that do not have any reactive nitroso groups. Also disclosed are use of the azo compounds as mediators in optical and electrochemical diagnostic methods, as well as detection reagents, kits and test elements that include such azo compounds and that can be used in the diagnostic methods. | 2014-07-31 |
20140212904 | Fluorescence Assay For Ghrelin O-Acyltransferase Activity - An assay to detect ghrelin O-acyltransferase activity using an acrylodan-labeled peptide mimic of ghrelin that provides for high-throughput screening for ghrelin O-acyltransferase inhibitors and detection via high performance liquid chromatography. Alternatively, the assay for ghrelin acylation may be based on a synthetic peptide substrate that mimics the N-terminal sequence of ghrelin and has an environmentally-sensitive fluorophore attached to its C-terminal amino acid through chemoselective ligation. | 2014-07-31 |
20140212905 | Novel Method for Detection of Beta-D-Glucuronidase - A method for detection of beta-D-glucuronidase activity in a sample that comprises the steps of (i) providing a glucuronic acid ester compound of the general formula (I) wherein R1 is a C1-4 alkyl group, OR2 is a dye moiety, which is liberated after cleavage of the glycosidic bond; (ii) contacting said glucuronic acid ester compound with a material of said sample exhibiting hydrolytic activity towards glucuronic acid esters, thereby removing R1 and thus forming a sample containing an indicator compound suitable for the detection and/or measurement of beta-D-glucuronidase activity, and (iii) using said indicator to perform an assay requiring detection or measurement of beta-D-glucuronidase activity. | 2014-07-31 |
20140212906 | Screening Method - A method for screening for variant peptides uses mass spectrometry (MS). A system and a kit may be used for performing the method. Proteins in a sample are digested to form a defined series of peptides. The defined series of peptides are ionized. The ionized species are subjected to collision induced dissociation. Species of known mass/charge ratio are detected to confirm the presence of the protein variant in the original sample. | 2014-07-31 |
20140212907 | Methods and Kits for Measuring Toxicity and Oxidative Stress in Live Cells - Compositions and methods for the measuring toxicity, antioxidant capacity, and oxidative stress are provided. | 2014-07-31 |
20140212908 | Fetal Pulmonary Cells and Uses Thereof - Isolated mixed populations of fetal pulmonary cells, engineered three-dimensional tissue constructs of these cells, and uses thereof in identifying therapeutic agents which augment, repair, and/or replace dysfunctional native lung and to perform in vitro studies such as pharmaceutical screening, models for lung development and disease and characterization of chemical or mechanical injury are provided. | 2014-07-31 |
20140212909 | Monolayer Stress Microscopy - Disclosed are systems, apparatus, devices and methods, including a method that includes determining traction forces exerted by a cellular monolayer on a substrate on which the monolayer is placed, and determining internal forces within and between cells of the monolayer based on the determined traction forces. In some embodiments, determining the internal forces of the cellular monolayer may include determining internal stresses within the cellular monolayer that act to balance the determined traction forces over at least part of the cellular monolayer. In some embodiments, determining of the internal stresses may also include setting boundary conditions at a boundary determined based on an optical field of view of an observed section of the monolayer. | 2014-07-31 |
20140212910 | MICROGELS AND MICROTISSUES FOR USE IN TISSUE ENGINEERING - The present invention features microgels and microtissues for use in tissue engineering. Featured is a microencapsulation device for making microgels and/or microtissues via an emulsion technology. Also featured are methods of making higher ordered structures that mimic in vivo tissue structures. Methods of us are also featured. | 2014-07-31 |
20140212911 | ONE TO ONE IDENTIFICATION SYSTEM - The present invention relates to a system arranged for assisting secure handling of cells in order to minimize the risk of handling mistakes resulting in mix-up of cell samples. The system allows for one to one identification of cells during a whole culturing period. This is obtained by providing a culturing system with loading/exit control means which ensures that only one device is capable of being loaded/removed at a time. | 2014-07-31 |
20140212912 | Purified Cell Population Isolated from Whole Blood - A purified solution of human plasma, MNCs and platelets isolated from whole blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue wherein said purified solution comprises at least 90% by number of the MNCs in said whole blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue, said purified solution comprises at most 10% by number of the red blood cells and granulocytes in said whole blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue, and said purified solution is isolated and separated from said whole blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue during a single centrifugation run. | 2014-07-31 |
20140212913 | EMBEDDING RESIN COMPOSITION FOR ELECTRON MICROSCOPE, AND METHOD FOR OBSERVING SAMPLE ON ELECTRON MICROSCOPE USING SAID COMPOSITION - The present invention provides an embedding resin composition for electron microscopy having satisfactory performance as an embedding medium, including embedding performance and sectioning quality, and exhibiting excellent antistatic performance; and a method for observing a sample with an electron microscope using the composition. The embedding resin composition for electron microscopy of the present invention having antistatic performance comprises an ionic liquid and an embedding medium comprising an epoxy-based resin, a methacrylate resin or an unsaturated polyester resin. Preferably, the ionic liquid comprising
| 2014-07-31 |
20140212914 | Method for Purifying Certain Cell Populations in Blood or Bone marrow by Depleting Others - A method for purifying and harvesting certain cell populations in blood or bone marrow by depleting at least one of red blood cells, granulocytes, or platelets from a sample comprising blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue is disclosed. The apparatus comprises a sterile, single use rigid, self-supporting cartridge within which the automated depletion, purification and harvesting of target cell populations occurs and all components may be distributed. | 2014-07-31 |
20140212915 | METHOD FOR PREPARING HUMAN NEOPLASTICALLY TRANSFORMED CELLS - A method for preparing neoplastically transformed cells from human-derived cells, including the step of introducing human telomerase catalytic subunit (hTERT) gene, SV40 small T antigen (SV40ST) gene, and an antisense oligonucleotide derived from human 28S rRNA into the human-derived cells. The method for preparing neoplastically transformed cells from human-derived cells can be utilized when a variety of human normal cells are induced to be neoplastically transformed in order to elucidate cancer onset mechanisms, so that the method can be effectively utilized in search of target molecules for a new medicament. | 2014-07-31 |
20140212916 | RAPID TEST FOR DETECTION OF INFECTION AND THERAPEUTIC RESPONSE - The present invention is directed to the use of isotopically labeled citrulline for gnosing the presence or absence of a bacterial infection in the lungs of a patient. | 2014-07-31 |
20140212917 | COMPENSATION OF MOTION-RELATED ERROR IN A STREAM OF MOVING MICRO-ENTITIES - Apparatus and methods for detecting, characterizing, and compensating motion-related error of moving micro-entities are described. Motion-related error may occur in streams of moving micro-entities, and may represent a deviation in and expected arrival time or an uncertainty in position of a micro-entity within the stream. Motion-related error of micro-entities is observed in a flow cytometer, e.g., as pulse jitter, and is found to have a functional dependence on a parameter of the system. The pulse jitter can be compensated, according to one embodiment, by adjusting data acquisition observation windows. For the flow cytometer, a reduction of pulse jitter can improve measurement accuracy, resolution of doublets, system throughput, and enable an increase in an interrogation region for probing the micro-entities. | 2014-07-31 |
20140212918 | IN VITRO MODEL OF MACROSTEATOTIC (FATTY) LIVER - The present invention relates to a system and methods for identifying a compound for de-fatting and functional recovery of macrosteatotic hepatocytes. | 2014-07-31 |
20140212919 | PROTEIN SYNTHESIS KIT, AND METHOD FOR EXPRESSING AND EXTRACTING PROTEINS USING AUTOMATIC EXTRACTION EQUIPMENT - Provided is a method of protein synthesis. The method of protein synthesis according to the present invention uses an automatic biological material purification apparatus including: a well plate kit; a heating part; and a magnetic field applying part, such that a plurality of target proteins may be more quickly and simply obtained as compared to target proteins obtained by using the existing method for expressing/purifying proteins through conventional cell culture, and a reproducible synthesis efficiency on the same proteins may be obtained due to no deviation between reaction wells. | 2014-07-31 |
20140212920 | MUTANT GAMMA-GLUTAMYLTRANSFERASE, AND A METHOD FOR PRODUCING GAMMA-GLUTAMYLVALYLGLYCINE OR A SALT THEREOF - A method for producing γ-Glu-Val-Gly comprising the step of reacting Val-Gly with a γ-glutamyl group donor in the presence of a γ-glutamyltransferase, a microorganism containing the enzyme, or a processed product thereof to generate γ-Glu-Val-Gly, wherein the γ-glutamyltransferase consists of a large subunit and a small subunit, and the small subunit has a specific mutation. | 2014-07-31 |
20140212921 | CELL CULTURES AND METHODS OF HUMAN RECOMBINANT GROWTH AND DIFFERENTIATON FACTOR-5 (RHGDF-5) - A cell cuture media for plasmid production is provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided herein is a method of making a cell culture media for plasmid production and a method for growing a plasmid vector. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed are cost-effective, time-saving and are of manufacturing quality. | 2014-07-31 |
20140212922 | TWO PLASMID MAMMALIAN EXPRESSION SYSTEM - Reverse engineering has offered new ways of studying the pathology of RNA viral infections, new more efficient devices of synthesizing recombinant viruses and developing vaccines and also demonstrated the versatility and efficiency of RNA dependent RNA polymerase RDRP system as an expression system. However, the currently used methods require a repertoire of complex, difficult-to-use tools. Present invention describes, a simpler plasmid based mammalian expression system that uses the RDRP enzyme activity for expression of recombinant proteins or RNA from viral minigenomes and rescue of recombinant viruses from cDNAs encoding entire genome(s) of negative stranded RNA viruses. This system will be useful for expression of recombinant proteins, therapeutic RNA molecules including anti-sense and/or selecting interfering RNA and Ribozymes. This system can also be used for gene therapy and producing recombinant viruses for production of new vaccines. | 2014-07-31 |
20140212923 | Protein Expression - The present invention relates to a genetically modified yeast cell comprising: —at least one recombinant promoter operably linked to at least one gene encoding a polypeptide or protein supporting the biosynthesis of polypeptides or proteins within said cell, said at least one gene being located at the native genomic locus of the genetically unmodified wild-type yeast cell, wherein the naturally occurring promoter of the at least one gene encoding the biosynthesis supporting polypeptide or protein is inactivated by at least one mutation within said naturally occurring promoter and, —a secretion cassette comprising a recombinant nucleic acid molecule encoding a protein or polypeptide of interest and a method for producing a recombinant protein or polypeptide of interest using such a cell. | 2014-07-31 |
20140212924 | HUMAN RECOMBINANT GROWTH AND DIFFERENTIATON FACTOR-5 (RHGDF-5) - Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for production and high expression of rhGDF-5 protein are disclosed herein. The methods of enhancing production and protein expression of rhGDF-5 protein as disclosed are cost-effective, time-saving and are of manufacturing quality. | 2014-07-31 |
20140212925 | IL-1 BINDING PROTEINS - Proteins that bind IL-1α and IL-1β are described along with their use in compositions and methods for treating, preventing, and diagnosing IL-1-related disorders and for detecting IL-1α and IL-1β in cells, tissues, samples, and compositions. | 2014-07-31 |
20140212926 | Improving the production of foreign proteins - The present invention concerns a method for improving the expression levels of proteins, wherein hydrophobin fusion proteins are expressed, thereby inducing the formation of protein bodies, and wherein said hydrophobin fusions are co-expressed with a target protein or with a further fusion of the target protein. | 2014-07-31 |
20140212927 | COMPOSITIONS COMPRISING CELLOBIOSE DEHYDROGENASE FROM PYCNOPORUS CINNABARINUS AND THEIR USE FOR THE DEGRADATION OF LIGNOCELLULOSIC BIOMASS - Enzyme compositions including at least a) a cocktail of cellulases from | 2014-07-31 |
20140212928 | METHODS AND COMPOSITIONS FOR TARGETED SINGLE-STRANDED CLEAVAGE AND TARGETED INTEGRATION - Disclosed herein are methods and compositions for generating a single-stranded break in a target sequence, which facilitates targeted integration of one or more exogenous sequences. | 2014-07-31 |
20140212929 | DNA POLYMERASES WITH INCREASED 3'-MISMATCH DISCRIMINATION - Disclosed are mutant DNA polymerases having increased 3′-mismatch discrimination relative to a corresponding, unmodified polymerase. The mutant polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the mutant DNA polymerases. | 2014-07-31 |
20140212930 | DIVERSIFICATION OF HUMAN MILK OLIGOSACCHARIDES (HMOs) OR PRECURSORS THEREOF - A method of diversification of human milk oligosaccharides (HMOs) or precursors thereof, compounds obtainable by the method, and uses and compositions involving such compounds. The method comprises a) providing at least one compound or a mixture of the compounds selected from the group consisting of: optionally sialylated and/or fucosylated lactose derivatives of general formula 2 and salts thereof; b) adding at least one enzyme comprising a transglycosidase activity to the at least one compound or a mixture of compounds provided according to step a); and c) incubating the mixture obtained according to step b). | 2014-07-31 |
20140212931 | Bacterial Iodoperoxidases from Zobellia Galactanivorans, Methods of Preparation and Uses Thereof - The present invention concerns iodoperoxidases from | 2014-07-31 |
20140212932 | Methods and compositions for degrading cellulosic material - The present invention relates to enzyme compositions comprising a polypeptide having cellobiohydrolase II activity, a polypeptide having xylanase activity, and one or more cellulolytic proteins and their use in the degradation or conversion of cellulosic material. | 2014-07-31 |
20140212933 | OVER-EXPRESSION OF A PUTATIVE OXIDOREDUCTASE (UCPA) FOR INCREASING FURFURAL OR 5-HYDROXYMETHYLFURFURAL TOLERANCE - The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural. | 2014-07-31 |
20140212934 | BIOLOGICAL SYNTHESIS OF DIFUNCTIONAL HEXANES AND PENTANES FROM CARBOHYDRATE FEEDSTOCKS - Provided herein are methods for the production of difunctional alkanes in microorganisms. Also provided are enzymes and nucleic acids encoding such enzymes, associated with the difunctional alkane production from carbohydrates feedstocks in microorganisms. The invention also provides recombinant microorganisms and metabolic pathways for the production of difunctional alkanes. | 2014-07-31 |
20140212935 | SHORT CHAIN FATTY ACIDS FROM BACTERIA - The present invention relates to an engineered bacteria for producing short chain fatty acid with the overexpression of a long chain (>C12) acyl-ACP thioesterases (long-TE) and a short chain (≦C12) acyl-ACP thioesterases (short-TE). | 2014-07-31 |
20140212936 | Solventless Extraction Process - The present invention provides a method for extracting lipids from microorganisms without using organic solvent as an extraction solvent. In particular, the present invention provides a method for extracting lipids from microorganisms by lysing cells and removing water soluble compound and/or materials by washing the lysed cell mixtures with aqueous washing solutions until a substantially non-emulsified lipid is obtained. | 2014-07-31 |
20140212937 | System and Method for Improved Gas dissolution - The invention provides a microbubble generation system with increased efficiency and flexibility compared to known systems. Further, the invention provides a method of microbubble generation. In particular, invention relates to increasing the efficiency of a fermentation reaction by reducing bubble size and increasing gas absorption into a liquid fermentation broth. | 2014-07-31 |
20140212938 | Dehydrogenase Variants and Polynucleotides Encoding Same - The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2014-07-31 |
20140212939 | CYTOCHROME P450S AND USES THEREOF - The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds. | 2014-07-31 |
20140212940 | MEMBRANES - Mixed matrix pervaporation membranes are described which include i) a matrix phase comprising a polymeric material, and ii) a zeolitic imidazolate framework (ZIF) dispersed in the matrix phase. In membranes described, the thickness of the membrane is greater than 0.5 μm. The membranes may in examples be used in a process for separating an organic compound from an aqueous liquid mixture. An example process includes contacting the liquid mixture on one side of a mixed matrix pervaporation membrane to cause the organic compound to permeate the mixed matrix membrane, and removing from the other side of the membrane a permeate composition comprising a portion of the organic compound which permeated the membrane. Example membranes described have relatively good selectivity for separation of the organic compound from the liquid mixture. | 2014-07-31 |
20140212941 | Designer Organisms for Photobiological Butanol Production from Carbon Dioxide and Water - The present invention provides a biosafety-guarded photobiological butanol production technology based on designer transgenic plants, designer algae, designer blue-green algae (cyanobacteria and oxychlorobacteria), or designer plant cells. The designer photosynthetic organisms are created such that the endogenous photobiological regulation mechanism is tamed, and the reducing power (NADPH) and energy (ATP) acquired from the photosynthetic process are used for synthesis of butanol (CH | 2014-07-31 |
20140212942 | PRODUCTION OF VOLATILE DIENES BY ENZYMATIC DEHYDRATION OF LIGHT ALKENOLS - Described is a method for generating conjugated dienes through a biological process. More specifically, the application describes a method for producing conjugated dienes (for example butadiene, isoprene or dimethylbutadiene) from light alkenols via enzymatic dehydration, in particular by making use of an alkenol dehydratase. | 2014-07-31 |
20140212943 | METHOD FOR IN-VITRO TREATMENT OF DIFFERENTIATED OR UNDIFFERENTIATED CELLS BY APPLICATION ELECTROMAGNETIC FIELDS - A process is described for the treatment of stem cells or differentiated cells by application of radiofrequency electro-magnetic fields, by means of an electromagnetic field generator ( | 2014-07-31 |
20140212944 | MOF-based hierarchical porous materials, methods for preparation, methods for pore regulation and uses thereof - A series of MOF-based hierarchical porous material, namely IPD-mesoMOF-1˜9, based on nanoscale MOFs of MIL-100(Al, Fe, Cr, Sc and In), MIL-53(Al), HKUST-1, DUT-5, DUT-4, MIL-101(Cr), MIL-101NDC(Cr), MIL-101BPDC(Cr) and MIL-110 respectively, forming the permanent interparticle porosities by using close (or relatively close) packing, and preparation methods thereof. Modulated or functionalized IPD-mesoMOFs can be applied for gas adsorption and molecule separation (such as CH4- and CO2-adsorption, gasoline/diesel desulfurization and purification), catalyst loadings and molecular recognition/immobilization of biological macromolecules and enzymes. | 2014-07-31 |
20140212945 | RNA TARGETING COMPOUNDS AND METHODS FOR MAKING AND USING SAME - Disclosed are RNA targeting compounds, methods for using the subject RNA targeting compounds to treat myotonic dystrophy and other diseases are also disclosed. | 2014-07-31 |
20140212946 | NON-NATURAL RIBONUCLEASE CONJUGATES AS CYTOTOXIC AGENTS - The present invention is directed toward the delivery of a toxic protein to pathogenic cells, particularly cancer cells. In preferred embodiments, the toxic protein is a ribonuclease that has been modified to make it toxic to target cells and that can be conjugated to a target cell-specific delivery vector, such as an antibody, for delivery to pathogenic cells. | 2014-07-31 |
20140212947 | ELECTRODE INCLUDING A SELF-ASSEMBLING POLYMER HAVING AN ORGANOMETAL, AND METHOD FOR MANUFACTURING SAME - The present invention relates to a bioelectrode including a cross-linkable organometallic polymer, and to a method for manufacturing same, and more particularly, to an electrode in which a nanostructure of the organometallic polymer is controlled to be used in bio fuel cells, biosensors, and the like. The electrode according to the present invention includes an organometal and further includes a self-assembling block copolymer and enzyme, and provides usages in bio fuel cells and biosensors. | 2014-07-31 |
20140212948 | XYLANASE COMPOSITION WITH INCREASED STABILITY - A xylanase composition and a method for manufacturing the xylanase composition are provided, wherein the xylanase composition comprises a xylanase and a stabilizer, and the xylanase is from an anaerobic fungus, the stabilizer comprises a polyol, and the content of the polyol is at least 40 wt %, based on the total weight of the xylanase composition. | 2014-07-31 |
20140212949 | METHOD FOR PREPARING MICROVESICULAR ADAM15 - The present invention relates to a method for preparing microvesicular ADAM15, comprising: (a) a step of activating protein kinase C (PKC) of separated mammalian cells; and (b) a step of separating microvesicle-containing ADAM15 from the mammalian cells. According to the present invention, a method for preparing microvesicular ADAM15 from mammalian cells is provided, and a novel cell regulation mechanism mediated by ADAM proteins is provided by the study of the function of the microvesicular ADAM15. Further, the present invention may provide a novel anti-cancer drug using microvesicular ADAM15, which inhibits adhesion, proliferation and migration of tumor cells. | 2014-07-31 |
20140212950 | METHOD TO INCREASE THE INFECTIVITY OF VIRUS PARTICLES - A method for enhancing infectivity of HCMV virus particles is provided. | 2014-07-31 |
20140212951 | EXPRESSION CONDITIONS AND METHODS OF HUMAN RECOMBINANT GROWTH AND DIFFERENTIATON FACTOR-5 (RHGDF-5) - A cell culture media for growing transformant pGDF-5-Trc-transformed cells is provided for increased production of transformant pGDF-5-Trc-transformed cells. Also provided herein are methods of growing the transformant pGDF-5-Trc-transformed cells. The methods of growing the transformant pGDF-5-Trc-transformed cells as disclosed are cost-effective, time-saving and are of manufacturing quality. | 2014-07-31 |
20140212952 | METHODS AND COMPOSITIONS OF HUMAN RECOMBINANT GROWTH AND DIFFERENTIATON FACTOR-5 (RHGDF-5) ISOLATED FROM INCLUSION BODIES - Expression vector systems are provided for increased production of a recombinant GDF-5 (rhGDF-5) protein. Also provided are transformed host cells that were engineered to produce and express high levels of rhGDF-5 protein. Methods for isolating recombinant GDF-5 protein from an inclusion body of a cell are disclosed herein. The methods as disclosed are cost-effective, time-saving and are of manufacturing quality. | 2014-07-31 |
20140212953 | YEAST MICROORGANISMS WITH REDUCED BY-PRODUCT ACCUMULATION FOR IMPROVED PRODUCTION OF FUELS, CHEMICALS, AND AMINO ACIDS - The present invention relates to recombinant microorganisms comprising biosynthetic pathways and methods of using said recombinant microorganisms to produce various beneficial metabolites. In various aspects of the invention, the recombinant microorganisms may further comprise one or more modifications resulting in the reduction or elimination of 3 keto-acid (e.g., acetolactate and 2-aceto-2-hydroxybutyrate) and/or aldehyde-derived by-products. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 2014-07-31 |
20140212954 | PHOTOBIOREACTOR - A photobioreactor system includes vessel for containing a biomass and a plurality of illuminators which may have solid state devices embedded therein or coupled thereto. The light energy is distributed in the vessel volume by the illuminators so as to reach substantially all of the biomass being circulated in the vessel. Biomass may exit the top of the vessel and be refreshed with nutrients and liquid before being reintroduced at the bottom of the vessel. Carbon dioxide is introduced at the bottom of the vessel. The biomass may be agitated either ultrasonically or by motion of the illuminators. A source of energy for the light sources may be solar panels with battery storage and the carbon dioxide may be a byproduct of thermal power generation. | 2014-07-31 |
20140212955 | CONSTRUCTION MATERIAL MADE OF ALGAE AND METHOD FOR PRODUCING THEREOF - The present disclosure describes use of filamentous algae to form insulating construction materials which provide thermal and noise insulation. Algae from the order Zygnematales, the Cladophorales, or the Ulotrichales can be dried and formed for use as insulating material. Algae mass can be combined into several layers, using a binder to attach the layers to each other. A composite material of algae mass and an additive can be used and form the body of insulation panels having honeycomb-shaped chambers, which are sealed by a foil that is laminated onto the body. | 2014-07-31 |
20140212956 | METHOD TO STIMULATE AND SUSTAIN THE ANAEROBIC BIODEGRADATION OF LIGHT NON-AQUEOUS PHASE LIQUID - A method for treating LNAPL source zones using a cost effective LNAPL source zone technology to degrade residual LNAPL, by introducing bioremediation amendments comprising nutritional supplements in quantities, locations, and depths required to stimulate and sustain the anaerobic biodegradation of an LNAPL source zone; monitoring the LNAPL source zone for adverse conditions that decrease anaerobic LNAPL biodegradation; eliminating any identified adverse conditions to sustain LNAPL biodegradation; and maintaining the water with nutritional supplements in quantities, locations, and depths required to stimulate and sustain the anaerobic biodegradation of LNAPL. | 2014-07-31 |
20140212957 | PRODUCTION OF OPTICALLY PURE PROPANE-1,2-DIOL - A method for producing optically pure propane-1,2-diol, including the method steps: a. hydrogenation of lactides, metal-catalysed heterogenous catalysis being carried out in the presence of hydrogen, a crude product containing propane-1,2-diol being produced, and b. dynamic, kinetic racemate resolution, propane-1,2-diol of an optical purity in the range of ≧99% e.e. being produced. | 2014-07-31 |
20140212958 | LYSIS AND REVERSE TRANSCRIPTION FOR MRNA QUANTIFICATION - The present invention is directed to a method for performing an RT-PCR for amplifying a target RNA including the steps of (i) cultivation of a population of adherent cells in a cell culture vessel (ii) lysis of the population of adherent cells which is supposed to contain the target RNA in the sample vessel with a lysis buffer comprising between 0.05 M and 1 M of a chaotropic agent (iii) adding reagents to the sample vessel which are necessary to perform a reverse transcription reaction such that the chaotropic agent is present in a concentration of about 10 to 60 mM in the sample vessel, and reverse transcribing the target RNA and (iv) amplifying the first strand cDNA by means of subjecting the sample to multiple cycles of a thermocycling protocol. | 2014-07-31 |
20140212960 | LEUKOCYTE MEASUREMENT DEVICE AND REAGENT KIT - A leukocyte measurement device is provided with a spread inspection window at a position separated from an opening part along a spreading direction of a blood-derived sample in a carrier, for making the carrier visible from outside. When the blood-derived sample is dropped from the opening part, leukocytes contained in the blood-derived sample are captured by the carrier in the vicinity of a dropping position, while erythrocytes and/or hemoglobin and the like spread through the carrier from the dropping position into a predetermined direction. Therefore, in the leukocyte measurement device, seeing through the spread inspection window the color of the erythrocytes and/or hemoglobin spread from the dropping position can determine whether it has already been used or not, whereby a simple structure can prevent measurers from erroneously reusing it. | 2014-07-31 |
20140212961 | Centrifuge For Separating A Sample Into At Least Two Components - The invention relates to a centrifuge for separating a sample into at least two components, comprising a chamber for receiving a sample to be centrifuged. According to the invention, the centrifuge further comprises a means for controlling the progress of the sample separation is located at the chamber. | 2014-07-31 |
20140212962 | LIVING CELL CRYOPRESERVATION TOOL - A living cell cryopreservation tool has a body part formed of a cold-resistant material and a living cell holding part formed of the cold-resistant material. The living cell holding part has a long and narrow living cell attaching and holding portion. The living cell attaching and holding portion has a plurality of living cell accommodation concave portions formed in a longitudinal direction thereof and a plurality of excess cryopreservation liquid discharge passages communicating with the living cell accommodation concave portions. | 2014-07-31 |
20140212963 | Device For Isolating A Fraction In A Biological Sample - A device and a method are provided for isolating a fraction in a biological sample. The fraction is bound to solid phase substrate to define a fraction-bound solid phase substrate. The device includes an input zone for receiving the biological sample therein and a second zone for receiving an isolation fluid therein. A force is provided that is generally perpendicular to gravity. The force is movable between a first position adjacent the input zone and a second position adjacent the isolation zone. The force captures the fraction-bound solid phase substrate and the fraction-bound solid phase substrate moves from the input zone to the isolation zone in response to the force moving from the first position to the second position. | 2014-07-31 |
20140212964 | MODULAR PLATFORM FOR MULTI-TISSUE INTEGRATED CELL CULTURE - The systems and methods disclosed herein are generally related to a cell culture system. More particularly, the systems and methods enable the culturing and interconnecting of a plurality of tissue types in a biomimetic environment. By culturing organ specific tissue types within a biomimetic environment and interconnecting each of the organ systems in a physiologically meaningful way, experiments can be conducted on in vitro cells that substantially mimic the responses of in vivo cell populations. In some implementations, the organ systems are fluidically connected with a constant-volume pump. | 2014-07-31 |
20140212965 | ISOLATED ADULT PLURIPOTENT STEM CELLS AND METHODS FOR ISOLATING AND CULTIVATING THEREOF - This invention relates to isolated pluripotent adult stem cells which are obtained from exocrine glandular tissue as well as methods of isolating and culturing these pluripotent stem cells. | 2014-07-31 |
20140212966 | PASSAGING AND HARVESTING FORMULATION AND METHOD FOR HUMAN PLURIPOTENT STEM CELLS - Formulations and methods are disclosed for the harvesting and subsequent passaging of human pluripotent stem cells without the use of enzymes and/or scraping to dislodge cells from cell culture vessels. The formulations and methods permit the harvesting of cells as large clusters from the surface of various cell culture vessels including multilayer cell culture vessels. Further, the formulations and methods provide high yields of harvested cells for subsequent passaging and high post-harvest cell viability. Pluripotent stem cells passaged with the formulations according to the methods remain undifferentiated and express typical stem cell markers, while, at the same time, they retain the differentiation capability and are able to differentiate into the cells in all three germ layers and generate teratomas, even after numerous rounds of harvesting and passaging. These hPSCs also maintain normal karyo-type after passaged with the formulations for extended period of time. | 2014-07-31 |
20140212967 | MICROFABRICATED POLYMERIC VESSEL MIMETICS - The present disclosure is directed to embodiments of microstructured membranes, methods of fabricating microstructured membranes, bioreactors housing microstructured membranes, and methods of using bioreactors and microstructured membranes. In some embodiments, the present disclosure allows culturing of cellular tissues in an environment which more accurately resembles a native environment. In some more specific embodiments, the present disclosure allows culturing of tumor cells on a membrane having a microfabricated pattern which mimics a native vasculature system. | 2014-07-31 |
20140212968 | SYNTHETIC MATRICES FOR LONG TERM EXPANSION OF PLURIPOTENT HUMAN EMBRYONIC STEM CELLS - Provided herein is a synthetic polymer-based hydrogel for the self-renewal and expansion of human stem cells such as embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Also provided are methods of making and using the same. | 2014-07-31 |
20140212969 | Nucleic Acids, Polypetides, and Methods for Modulating Apoptosis - The present invention relates to isolated and/or purified rat apoptosis-specific eucaryotic initiation Factor-5A (eIF-5A) and deoxyhypusine synthase (DHS) nucleic acids and polypeptides. The present invention also relates to methods of modulating apoptosis using apoptosis-specific eIF-5A and DHS, and antisense oligonucleotides and expression vectors of apoptosis-specific and DHS useful in such methods. | 2014-07-31 |
20140212970 | METHOD AND KIT FOR CULTURING STEM CELLS - A method is provided, including adding a phthalide to a stem cell medium to provide a phthalide-containing medium, and then culturing a stem cell using the phthalide-containing medium. The use of a phthalide in a medium for culturing stem cells optionally maintains the pluripotency of stem cells. The phthalide also enhances the generation efficiency of induced pluripotent stem cells to decrease the culture cost. | 2014-07-31 |
20140212971 | Mast Cell Culture and Methods to Produce the Culture - The present invention relates to mast cell cultures that are derived from hematopoietic progenitors and the use thereof. The invention describes a method for generating in-vitro cultures of human mast cells with functional phenotype of connective tissue-type mast cells. By monitoring the levels of chemokines released into the medium, such mast cell cultures can be used as a cell-based assay to assess regulation of mast cell functions and pharmacological activities of tryptase inhibitors. | 2014-07-31 |
20140212972 | PROCESS FOR PRODUCING CYTOTOXIC LYMPHOCYTE - The present invention relates to a method for preparing a cytotoxic lymphocyte characterized in that the method comprises the step of carrying out at least one of induction, maintenance and expansion of a cytotoxic lymphocyte in the presence of fibronectin, a fragment thereof or a mixture thereof. | 2014-07-31 |
20140212973 | TEMPERATURE-RESPONSIVE SUBSTRATE FOR CELL CULTURE AND PRODUCTION METHOD THEREOF - To form a temperature-responsive surface for cell culture by simple processes, said temperature-responsive surface for cell culture being capable of efficiently culturing cells. Cultured cells or a cell sheet can be efficiently removed from the temperature-responsive surface for cell culture by merely changing the temperature of the substrate surface. To coat the substrate surface with a block copolymer, in which a water insoluble polymer segment is coupled with a temperature-responsive polymer segment, in an amount of 0.8 to 3.0 μg/cm | 2014-07-31 |
20140212974 | CELL CULTURE MEMBRANE, CELL CULTURE SUBSTRATE, AND METHOD FOR MANUFACTURING CELL CULTURE SUBSTRATE - Provided is: a cell culture membrane, which is free from materials derived from living organisms, can easily be industrially mass-produced, exhibits superior long-term storage properties and chemical resistance, has excellent cell adhesion properties and long-term culture properties and is capable of replicating a cell adhesion morphology that is similar to that of collagen derived from living organisms and being used for conventional cell cultivation. Also provided are a cell culture substrate, and a method for manufacturing the cell culture substrate. In the present invention, as a cell adhesion layer, a polymer membrane represented by formula (I) is formed on the base of a cell culture substrate so as to have a membrane thickness equal to or greater than 0.2 μm (in the formula, R1 and R2 represent a —(CH | 2014-07-31 |
20140212975 | COMPOSITIONS AND METHODS FOR BIOCULTURE OF WASABIA JAPONICA - The present invention provides media, kits, systems, and methods for achieving large scale wasabi production within a short time via bioculture. The present invention for wasabi production results in shorter tuber development phase and higher yield. | 2014-07-31 |
20140212976 | Recombinant microorganisms comprising NADPH dependent enzymes and methods of production therefor - The invention provides a recombinant carboxydotrophic Clostridia microorganism with increased overall utilization of NADPH relative to a parent microorganism. Further, the invention provides a method of producing a recombinant carboxydotrophic Clostridia microorganism which exhibits increased NADPH utilization relative to a parental microorganism. In particular, the invention relates to increasing the overall utilization of NADPH in a recombinant carboxydotrophic Clostridia microorganism in order to increase the production of at least one fermentation product by the microorganism. | 2014-07-31 |
20140212977 | Methods For Producing Multiple Recombinant Polypeptides In A Filamentous Fungal Host Cell - The present invention relates to methods for constructing a filamentous fungal strain for production of multiple recombinant polypeptides having biological activity. The present invention also relates to methods for producing multiple recombinant polypeptides having biological activity in a filamentous fungal strain. The present invention also relates to filamentous fungal strains expressing multiple recombinant polypeptides having biological activity. | 2014-07-31 |
20140212978 | SYSTEMS AND METHODS TO MONITOR OPERATING PROCESSES - Example methods, systems, and computer readable media are provided for monitoring operating processes. An example method includes monitoring an equipment parameter associated with an asset in an operating process unit and monitoring a process parameter associated with the asset. The example method includes determining an asset health corresponding to the asset. The asset health is determined based on a potential state of corrosion associated with the asset by comparing a corrosivity index to a corrosion threshold. The corrosivity index is a function of at least one of current values or changes in the monitored equipment parameter or the monitored process parameter over time. | 2014-07-31 |
20140212979 | DIFFUSION BASED METAL OXIDE GAS SENSOR - A measuring device is provided for determining the type and/or concentration a gaseous analyte from a set of analytes in a gaseous carrier. It comprises a housing having a passage to a cavity. A gas sensor with a heated metal-oxide sensing layer is arranged in the cavity. In order to gain a better understanding of the type of the analyte, diffusion effects are exploited by taking into account that the diffusion process through the passage as well as the catalytic reaction rate at the metal-oxide sensing layer depend on the type of the analyte. These material parameters can be determined by taking several measurements in a non-steady state of the concentration of the analyte within the cavity or while varying the reaction rate. | 2014-07-31 |