25th week of 2019 patent applcation highlights part 29 |
Patent application number | Title | Published |
20190185840 | Thermophilic L-asparaginase Mutant and Screening and Fermentation Methods Thereof - The present disclosure discloses a thermophilic L-asparaginase mutant and screening and fermentation methods thereof, and belongs to the field of gene engineering, enzyme engineering and fermentation engineering. In | 2019-06-20 |
20190185841 | XANTHAN LYASE VARIANTS AND POLYNUCLEOTIDES ENCODING SAME - The present invention relates to xanthan lyase variants and methods for obtaining xanthan lyase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2019-06-20 |
20190185842 | METHOD FOR OBTAINING ACTIVE INSOLUBLE XYLOSE ISOMERASE - The invention is in the field of biotechnology and involves recombinant DNA technology. It provides means and methods for obtaining an insoluble active fusion protein comprising xylose isomerase activity. More in particular, the invention relates to a method for obtaining active insoluble xylose isomerase, comprising the expression in a host organism of a recombinant gene encoding a fusion protein comprising a xylose isomerase in combination with a PPIase, thereby obtaining the active insoluble xylose isomerase. It also provides recombinant fusion proteins comprising xylose isomerase activity as well as their use in converting xylose to xylulose and glucose to fructose. | 2019-06-20 |
20190185843 | AN ASSEMBLY SYSTEM FOR A EUKARYOTIC CELL - The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. | 2019-06-20 |
20190185844 | Tuning CRISPR/CAS9 Activity with Chemically Modified Nucleotide Substitutions - The present disclosure provides CRISPR/Cas9 ribonucleoprotein compositions comprising chemically modified CRISPR RNA (crRNA) guide and trans-acting CRISPR RNA (tracrRNA) components. Methods of using the disclosed CRISPR/Cas9 ribonucleoprotein compositions are also provided. | 2019-06-20 |
20190185845 | APTAMER METHODS AND COMPOSITIONS - Methods of selecting an aptamer that specifically binds to a target molecule complexed with a derivatization agent. Also disclosed are specific aptamers and methods of use thereof. | 2019-06-20 |
20190185846 | AUTOMATED CELL PROCESSING METHODS, MODULES, INSTRUMENTS, AND SYSTEMS - In an illustrative embodiment, automated multi-module cell editing instruments are provided to automate multiple edits into nucleic acid sequences inside one or more cells. | 2019-06-20 |
20190185847 | Improving a Microorganism by CRISPR-Inhibition - The present invention relates to methods for improving at least one property of a microorganism host cell by repressing the expression of one or more genome target sequence of interest by CRISPR-inhibition as well as the resulting host cells and methods of production emplying said host cells. | 2019-06-20 |
20190185848 | MEANS AND METHODS FOR MODIFYING MULTIPLE ALLELES - The present invention relates to a method of modifying at least one gene in a cell via CRISPR/Cas, wherein the at least one gene has at least three alleles. The present invention further relates to cells obtainable by the method of the invention. Additionally, the present invention provides a method of producing a protein in a cell obtainable by the method of modifying at least one gene of the invention. Moreover, the invention relates to proteins obtainable by the method of producing a protein and use thereof, for example in therapy. | 2019-06-20 |
20190185849 | COMPOSITIONS AND METHODS FOR GENE EDITING - The present application provides materials and methods for treating a patient with one or more conditions associated with ANGPTL4 whether ex vivo or in vivo. In addition, the present application provides materials and methods for editing and/or modulating the expression of ANGPTL4 gene in a cell by genome editing. | 2019-06-20 |
20190185850 | SINGLE GUIDE RNA/CRISPR/CAS9 SYSTEMS, AND METHODS OF USE THEREOF - The present disclosure relates to single guide RNA (sgRNA), Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associate protein 9 (Cas9) system, and methods of use thereof for preventing, ameliorating or treating corneal dystrophies. | 2019-06-20 |
20190185851 | EXOSOME SECRETION INHIBITOR - An exosome secretion inhibitor containing a substance suppressing expression of NAPG, HINT3 or GXYLT1 gene, or a substance suppressing activity of NAPG, HINT3 or GXYLT1 protein. It is possible to suppress secretion of exosome, thereby making it possible to suppress proliferation or metastasis of cancer cells, so that the substance suppressing expression of the gene or activity of the protein can be used as an active ingredient of a pharmaceutical composition suitably used in treatment of cancer or suppression of cancer metastasis, and also the substance can provide a carcinostatic agent having a new action mechanism which specifically suppresses expression of the gene or activity of the protein. | 2019-06-20 |
20190185852 | THERAPEUTICALLY MODULATING APOB AND APOAI - MicroRNAs can be used to decrease expression of apolipoprotein B (apoB), increase expression of apolipoprotein A (apoA), and decrease expression of NCOR1. Use of these microRNAs can simultaneously reduce LDL and increase HDL in circulation and have applications in prevention and treatment of atherosclerosis, hyperlipidemia, and cardiovascular disease as well as other disorders associated with high apoB and/or low apoAI levels. | 2019-06-20 |
20190185853 | AAV-BASED TREATMENT OF CHOLESTEROL-RELATED DISORDERS - The invention in some aspects relates to methods and compositions for assessing the effectiveness of miRNA inhibitors. In other aspects of the invention, methods and compositions for treating cholesterol related disorders are provided. In one aspect of the invention, miRNA inhibitors against miR-122 and rAAV-based compositions comprising the same are provided. | 2019-06-20 |
20190185854 | Micro-RNA Delivery Compositions, Devices, and Methods - Provided herein are compositions that include a metal nanoparticle functionalized with a miRNA and a targeting molecule. The compositions may be used to prevent or reduce the rate of metastasis of cancer cells. The compositions also may include a drug, such as a chemotherapeutic agent. The compositions also may include a hydrogel in which the metal nanoparticles are dispersed. Methods of miRNA and/or drug delivery and kits also are provided. | 2019-06-20 |
20190185855 | CONJUGATED OLIGONUCLEOTIDES - Provided herein are conjugated oligonucleotides that are characterized by efficient and specific tissue distribution. | 2019-06-20 |
20190185856 | Synthetic Near-Threshold Translational Repressors - Provided herein are synthetic nucleic acid molecules and methods of using such synthetic nucleic acid molecules for strong repression of target gene expression. In particular, provided herein are methods for altering expression of a protein in a cell, where the method comprises introducing into a cell a protein coding sequence operably linked to a near-threshold translational repressor having first and second trigger recognition sequences that are fully or partially complementary to a repressing trigger RNA; and introducing into a cell the repressing trigger RNA. | 2019-06-20 |
20190185857 | NOVEL PROMOTER AND USE THEREOF - The present invention relates to a novel promoter, a vector comprising the promoter, a microorganism comprising the promoter or the vector, and a method for producing a target product using the microorganism. | 2019-06-20 |
20190185858 | APTAMER THERAPEUTICS USEFUL IN THE TREATMENT OF COMPLEMENT-RELATED DISORDERS - The invention provides nucleic acid therapeutics and methods for using these nucleic acid therapeutics in the treatment of complement-related disorders. | 2019-06-20 |
20190185859 | RNA FOR CANCER THERAPY - The present invention relates to RNA, particularly an immunostimulatory RNA (isRNA), a coding RNA or a combination thereof, for use in the treatment or prophylaxis of a disease, in particular a tumor and/or cancer disease. The present invention also provides pharmaceutical compositions, and a kit comprising the RNA(s). Further, the invention also comprises medical uses of the RNA(s) and compositions comprising the RNA(s). | 2019-06-20 |
20190185860 | Manipulated Immunoregulatory Element And Immunity Altered Thereby - The present invention relates to an artificially manipulated immune system having an improved immune effect. More particularly, the present invention relates to an immune system having functions artificially altered which comprises artificially manipulated immunoregulatory elements and cells containing the same. Contemplated according to a particular embodiment is an immune system comprising artificially manipulated immunoregulatory genes such as PD-1, CTLA-4, A20, DGKα, DGKζ, FAS, EGR2, PPP2R2D, PSGL-1, KDM6A, and TET2, and/or expression products thereof. | 2019-06-20 |
20190185861 | MULTI-CONJUGATE OF SIRNA AND PREPARING METHOD THEREOF - The present invention relates to a multi-conjugate of small interfering RNA (siRNA) and a preparing method of the same, more precisely a multi-conjugate of siRNA prepared by direct binding of double stranded sense/antisense siRNA monomers or indirect covalent bonding mediated by a cross -linking agent or a polymer, and a preparing method of the same. The preparing method of a siRNA multi-conjugate of the present invention is characterized by simple and efficient reaction and thereby the prepared siRNA multi -conjugate of the present invention has high molecular weight multiple times the conventional siRNA, so that it has high negative charge density, suggesting that it has excellent ionic interaction with a cationic gene carrier and high gene delivery efficiency. | 2019-06-20 |
20190185862 | CCR3 MODULATION IN THE TREATMENT OF AGING-ASSOCIATED IMPAIRMENTS, AND COMPOSITIONS FOR PRACTICING THE SAME - Methods of treating an adult mammal for an aging-associated impairment are provided. Aspects of the methods include modulating CCR3, e.g., by modulating eotaxin-1/CCR3 interaction, in the mammal in a manner sufficient to treat the mammal for the aging-associated impairment. A variety of aging-associated impairments may be treated by practice of the methods, which impairments include cognitive impairments. | 2019-06-20 |
20190185863 | EXPRESSION FROM TRANSPOSON-BASED VECTORS AND USES - Recombinant expression vectors are disclosed that include a control sequence for recombinant expression of proteins of interest; the control sequence combines a mCMV enhancer sequence with a rat EF-1alpha intron sequence. Some of the vectors are useful for tetracycline-inducible expression. Some of the vectors contain a 5′ PiggyBac ITR and a 3′ PiggyBac ITR to promote genomic integration into a host cell chromosome. A method of selecting a stable production cell line for manufacturing a protein of interest is also disclosed. Also disclosed are mammalian host cells comprising the inventive recombinant expression vectors and a method of producing a protein of interest, in vitro, involving the mammalian host cell. | 2019-06-20 |
20190185864 | COMPOSITIONS AND METHODS FOR TREATING NON-AGE-ASSOCIATED HEARING IMPAIRMENT IN A HUMAN SUBJECT - Provided herein are compositions that include at least two different nucleic acid vectors, where each of the at least two different vectors includes a coding sequence that encodes a different portion of an otoferlin protein, and the use of these compositions to treat hearing loss in a subject. | 2019-06-20 |
20190185865 | USE OF PROKARYOTIC TRANSCRIPTIONAL ACTIVATORS AS METABOLITE BIOSENSORS IN EUKARYOTIC CELLS - The present invention relates to the use of transcriptional activators from prokaryotic organisms for use in eukaryotic cells, such as yeast as sensors of intracellular and extracellular accumulation of a ligand or metabolite specifically activating this transcriptional activator in a eukaryot, such as yeast cell, such as a cell engineered to produce this ligand. The transcriptional activator controls a promoter upstream of one or more gene, which may include e.g. a reporter gene that may be a fluorescence marker, such as luciferase, green fluorescent protein or a gnee encoding antibiotic resistance. | 2019-06-20 |
20190185866 | REGULATABLE PROMOTER - A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system. | 2019-06-20 |
20190185867 | COMPOSITIONS AND METHODS TO CONTROL INSECT PESTS - The present invention relates generally to methods of molecular biology and gene silencing to control pests. | 2019-06-20 |
20190185868 | CONSTRUCTS AND METHODS FOR BIOSYNTHESIS OF CYCLOPAMINE - The present disclosure relates generally to the identification of enzymes within the cyclopamine biosynthesis pathway as well as to engineering transgenic plants or organisms for the production of cyclopamine. | 2019-06-20 |
20190185869 | P. SOMNIFERUM PLANTS FOR THE PRODUCTION OF CODEINE - There are provided plants of | 2019-06-20 |
20190185870 | ENGINEERED NUCLEASES TO GENERATE DELETION MUTANTS IN PLANTS - Methods are provided to mutate, in a targeted manner, the genome of a plant cell using a double stranded DNA break inducing enzyme. Also provided are plants, in particular | 2019-06-20 |
20190185871 | PLANTS WITH MODIFIED TRAITS - The present invention relates, inter alia, to vegetative plant parts, such as from a | 2019-06-20 |
20190185872 | METHOD FOR IMPROVING STEM VOLUME GROWTH AND BIOMASS PRODUCTION IN TREES - The present invention relates to a genetic construct comprising a nucleic acid sequence encoding cytokinin biosynthetic isopentenyl-transferase enzyme (IPT) operable linked to a promoter allowing expression of said nucleic acid sequence in cambial cells. The invention relates also a method for producing a transgenic plant capable of increased biomass production and/or increased stem volume growth compared to wild type plant and a method for improving the production of biomass and/or increased stem volume growth in trees, as well as to a tree that over expresses an endogenous or exogenous nucleic acid sequence encoding IPT in cambial cells and a wood product obtainable from the transgenic tree. | 2019-06-20 |
20190185873 | Methods and Compositions for PPO Herbicide Tolerance - The invention relates to biotechnology and provides novel recombinant DNA molecules and engineered proteins for conferring tolerance to protoporphyrinogen oxidase-inhibitor herbicides. The invention also provides herbicide tolerant transgenic plants, seeds, cells, and plant parts containing the recombinant DNA molecules, as well as methods of using the same. | 2019-06-20 |
20190185874 | LATE SEASON APPLICATION OF GLYPHOSATE FOR WEED CONTROL IN BRASSICA - The invention provides plants comprising transgenic event MON 88302 that exhibit tolerance to glyphosate herbicide. The invention also provides seeds, plant parts, cells, commodity products, and methods related to the event. The invention also provides DNA molecules that are unique to the event and were created by the insertion of transgenic DNA into the genome of a | 2019-06-20 |
20190185875 | METHODS AND COMPOSITIONS TO ENHANCE PLANT BREEDING - The present invention provides breeding methods and compositions to enhance the germplasm of a plant. The methods describe the identification and accumulation of transgenes and favorable haplotype genomic regions in the germplasm of a breeding population of crop plants. | 2019-06-20 |
20190185876 | METHODS AND COMPOSITIONS TO ENHANCE PLANT BREEDING - The present invention provides breeding methods and compositions to enhance the germplasm of a plant. The methods describe the identification and accumulation of transgenes and favorable haplotype genomic regions in the germplasm of a breeding population of crop plants. | 2019-06-20 |
20190185877 | ANTI-MICROBIAL PROTEINS - The disclosure provides polynucleotide molecules encoding novel defensins conferring increased pest tolerance and/or pesticidal activity when expressed in a plant, and recombinant DNA constructs and vectors comprising these molecules. Methods of making transgenic plants comprising recombinant defensin-encoding polynucleotide molecules and constructs, and transgenic plants, plant parts and seeds produced by these methods are provided. Compositions comprising one or more novel defensins of the disclosure are also provided having pesticidal and/or anti-microbial activity, as well as methods of their use. | 2019-06-20 |
20190185878 | SPINACH PLANTS THAT ARE RESISTANT TO DOWNY MILDEW - The present invention relates to the field of spinach breeding, in particular to a new dominant resistance gene, designated RPF12, which confers resistance against all races of | 2019-06-20 |
20190185879 | PLANTS COMPRISING WHEAT G-TYPE CYTOPLASMIC MALE STERILITY RESTORER GENES, MOLECULAR MARKERS AND USES THEREOF - Methods are described for selecting or producing a cereal plant comprising a functional restorer gene for wheat G-type cytoplasmic male sterility and nucleic acids for use therein. | 2019-06-20 |
20190185880 | TRANSPOSON SYSTEM AND METHODS OF USE - Disclosed are methods for the ex-vivo genetic modification of an immune cell comprising delivering to the immune cell, (a) a nucleic acid or amino acid sequence comprising a sequence encoding a transposase enzyme and (b) a recombinant and non-naturally occurring DNA sequence comprising a DNA sequence encoding a transposon. | 2019-06-20 |
20190185881 | INDUCIBLE EXPRESSION FROM TRANSPOSON-BASED VECTORS AND USES - Recombinant expression vectors are disclosed that include a control sequence for recombinant expression of proteins of interest; the control sequence combines a mCMV enhancer sequence with a rat EF-1alpha intron sequence. Some of the vectors are useful for tetracycline-inducible expression. Some of the vectors contain a 5′ PiggyBac ITR and a 3′ PiggyBac ITR to promote genomic integration into a host cell chromosome. A method of selecting a stable production cell line for manufacturing a protein of interest is also disclosed. Also disclosed are mammalian host cells comprising the inventive recombinant expression vectors and a method of producing a protein of interest, in vitro, involving the mammalian host cell. | 2019-06-20 |
20190185882 | Compositions and Methods of Delivering Treatments for Latent Viral Infections - The invention provides delivery methods and compositions for antiviral therapeutics. Methods and compositions are provided for targeted delivery of antiviral therapeutics into cells of interest using, for example, viral vectors such as adenovirus, AAV, and replication incompetent HSV. These and other delivery systems can be used as vehicles to deliver DNA vectors encoding a nuclease or a cell-killing gene. These delivery methods can also be used to deliver naked DNA or RNA, protein products, plasmids containing a promoter that is active only in a latent viral state which drives a cell-killing gene, or other therapeutic agents. | 2019-06-20 |
20190185883 | CAS9 PROTEINS INCLUDING LIGAND-DEPENDENT INTEINS - Some aspects of this disclosure provide compositions, methods, systems, and kits for controlling the activity of RNA-programmable endonucleases, such as Cas9, or for controlling the activity of proteins comprising a Cas9 variant fused to a functional effector domain, such as a nuclease, nickase, recombinase, deaminase, transcriptional activator, transcriptional repressor, or epigenetic modifying domain. For example, the inventive proteins provided comprise a ligand-dependent intein, the presence of which inhibits one or more activities of the protein (e.g., gRNA binding, enzymatic activity, target DNA binding). The binding of a ligand to the intein results in self-excision of the intein, restoring the activity of the protein. | 2019-06-20 |
20190185884 | PRODUCTION OF PRODUCTS WITH FAVOURABLE GHG EMISSION REDUCTIONS FROM CELLULOSIC FEEDSTOCKS - The present invention provides a process for producing one or more products for use as a transportation or heating fuel. In various embodiments the process comprises treating a cellulosic feedstock in one or more processing steps that release extractives from the feedstock. A solids-liquid separation is subsequently conducted on the process stream comprising the extractives and solids. An aqueous stream comprising one or more of the extractives may be fed to an anaerobic digester to produce crude biogas from which one or more impurities may optionally be removed. In various embodiments the process further comprises providing a solids stream to a thermal process. A product produced or derived from the thermal process may displace a product made from fossil fuel. One or more products obtained or derived from at least one of the foregoing process steps are provided for use as a transportation or heating fuel. In various embodiments the process enables advantaged fuel credit generation. | 2019-06-20 |
20190185885 | DRY-MILLING PROCESS - A dry-milling process for the production of dried distiller's grains with solubles (“DDGS”) includes the steps of dry-milling corn kernels to form a corn flour comprising corn fiber; combining the corn flour with water to form a mash; separating the corn fiber from the mash; treating the separated corn fiber with a composition; combining the treated corn fiber with the mash having the corn fiber separated therefrom to form a slurry; fermenting the slurry to produce beer and carbon dioxide; distilling the beer to produce ethanol and whole stillage; and processing the whole stillage to produce DDGS. The composition includes an alkanesulfonic acid, water, an enzyme, and optionally a surfactant. | 2019-06-20 |
20190185886 | METHODS & SYSTEMS FOR PROPAGATING MICROORGANISMS ON STILLAGE COMPOSITIONS - Provided are methods and systems of propagating a microorganism on a stillage composition. The methods involve growing microorganisms in a propagation medium formed from a polysaccharide-containing stillage composition with the majority of the of the polysaccharides in the propagation medium coming from the stillage composition. The propagation medium also includes cellulases and/or amylases to form monosaccharides from the polysaccharides. A first cell mass is grown in the propagation medium to form a second cell mass which is greater than the first cell mass. | 2019-06-20 |
20190185887 | PROCESS FOR USING BIOGENIC CARBON DIOXIDE DERIVED FROM NON-FOSSIL ORGANIC MATERIAL - The present disclosure provides a process for forming a biogenic carbon-based fuel or a fuel intermediate from biogenic carbon dioxide and hydrogen. At least a portion of the biogenic carbon dioxide and hydrogen is subjected to a reverse water gas shift reaction that produces at least carbon monoxide. The carbon monoxide so produced, the biogenic carbon dioxide and the hydrogen are introduced, together or separately, to a biologic or chemical conversion process to produce the fuel or fuel intermediate. | 2019-06-20 |
20190185888 | MICROORGANISMS AND METHODS FOR THE BIOLOGICAL PRODUCTION OF ETHYLENE GLYCOL - The invention provides genetically engineered microorganisms and methods for the biological production of ethylene glycol and precursors of ethylene glycol. In particular, the microorganism of the invention produces ethylene glycol or a precursor of ethylene glycol through one or more of 5,10-methylenetetrahydrofolate, oxaloacetate, citrate, malate, and glycine. The invention further provides compositions comprising ethylene glycol or polymers of ethylene glycol such as polyethylene terephthalate. | 2019-06-20 |
20190185889 | Methods for Making L-Glufosinate - Methods for the production of L-glufosinate (also known as phosphinothricin or (S)-2-amino-4-(hydroxy(methyl)phosphonoyl)butanoic acid) are provided. The methods comprise a two-step process. The first step involves the oxidative deamination of D-glufosinate to PPO (2-oxo-4-(hydroxy(methyl)phosphinoyl)butyric acid). The second step involves the specific amination of PPO to L-glufosinate, using an amine group from one or more amine donors. By combining these two reactions, the proportion of L-glufosinate in a mixture of L-glufosinate and D-glufosinate can be substantially increased. | 2019-06-20 |
20190185890 | METHOD FOR THE FERMENTATIVE PRODUCTION OF L-LYSINE - The present invention makes available novel L-lysine excreting bacteria of the species | 2019-06-20 |
20190185891 | METHOD FOR PRODUCING FRUCTOSE FROM GLUCOSE OBTAINED FROM LIGNOCELLULOSIC BIOMASS - The invention is in the field of enzymology. More in particular, it provides a method for the isomerization of glucose into fructose wherein the glucose is derived from lignocellulosic material. More in particular, it provides a method for converting glucose into fructose comprising the steps of: providing a composition comprising water, glucose and lignin, enzymatically converting the glucose to fructose in the presence of a glucose isomerase, and optionally purifying the fructose from the solution, wherein the glucose isomerase comprises an amino acid sequence that is at least 90% identical with the sequence according to SEQ ID NO: 1 or SEQ ID NO: 2. | 2019-06-20 |
20190185892 | METHOD FOR PRODUCING XYLULOSE FROM XYLOSE OBTAINED FROM LIGNOCELLULOSIC BIOMASS - The invention is in the field of enzymology. More in particular, it provides a method for the isomerization of xylose into xylulose wherein the xylose is derived from lignocellulosic material. More in particular, it provides a method for converting xylose into xylulose comprising the steps of: providing a composition comprising water, xylose and lignin, enzymatically converting the xylose to xylulose in the presence of a xylose isomerase, and optionally purifying the xylulose from the solution, wherein the xylose isomerase comprises an amino acid sequence that is at least 90% identical with the sequence according to SEQ ID NO: 1 or SEQ ID NO: 2 | 2019-06-20 |
20190185893 | ALPHA-1,3-GLUCAN GRAFT COPOLYMERS - Compositions are disclosed herein comprising a graft copolymer that comprises: (i) a backbone comprising dextran that has been modified with about 1%-25% alpha-1,2 branches, and (ii) one or more alpha-1,3-glucan side chains comprising at least about 50% alpha-1,3 glycosidic linkages. Further disclosed are reactions for producing such graft copolymers, as well as their use in derivatives, films and various other applications. | 2019-06-20 |
20190185894 | PROCESS FOR SUGAR MODULATION - The present invention relates to a method for producing a food product comprising hydrolysed starch, as well as to products obtainable by the method. The method has the advantage of reducing the amount of sugar (i.e. maltose) produced by hydrolysis as compared to conventional methods of starch hydrolysis and present the additional advantage of providing good processability for the food product. | 2019-06-20 |
20190185895 | DNA-MN HYBRID PARTICLES AND METHOD OF MANUFACTURING THE SAME - This invention relates to DNA-Mn hybrid particles and a method of manufacturing the same, the method including producing a circular DNA template for replication and forming particles in which DNA and Mn are bound to each other using Mn during the synthesis of a new strand of DNA from the circular DNA template for replication using a DNA polymerase, thus promoting the activity of the DNA polymerase using the coenzyme function of Mn and broadening the range of application fields of DNA as a biomaterial. | 2019-06-20 |
20190185896 | Stimulation of Regenerative Processes and Factors by Noble Gases - The invention provides compositions, treatment means and protocols for induction of regenerative processes by administration of noble gases. In one particular embodiment the invention provides the stimulation of production of factors associated with augmentation of hematopoiesis, angiogenesis, and wound healing by exposure of cells, organs, or mammals to a noble gas. In a particular embodiment the invention provides the administration of argon as a noble gas capable of upregulating production of VEGF and angiopoietin. | 2019-06-20 |
20190185897 | METHODS FOR MODULATING PROTEIN GLYCOSYLATION PROFILES OF RECOMBINANT PROTEIN THERAPEUTICS USING MONOSACCHARIDES AND OLIGOSACCHARIDES - The present invention relates to the field of protein production, and in particular to methods and compositions for modulating glycosylation of proteins expressed in host cells. | 2019-06-20 |
20190185898 | DESIGNER alpha 6-FUCOSIDASE MUTANTS ENABLE DIRECT CORE FUCOSYLATION OF INTACT N-GLYCOPEPTIDES AND N-GLYCOPROTEINS - The present invention provides for novel fucosidase mutants that server as fuco-ligases for core fucosylation of a range of biological glycopeptides and glycoproteins including intact therapeutic antibodies. Several mutants with mutation at the general acid/base residue E274 of the | 2019-06-20 |
20190185899 | Method for Producing 9alpha-Hydroxy Androstane-4-Alkene-3,17-Diketone by Enzymatic Conversion - The present disclosure discloses a method for producing 9α-hydroxy androstane-4-alkene-3,17-diketone by enzymatic conversion, and belongs to the fields of gene engineering and enzyme engineering. According to the present disclosure, oxidation subunit KshA, reduction subunit KshB and unknown active subunit KshC of 3-ketosteroid-9α-hydroxylase sourcing from | 2019-06-20 |
20190185900 | IN VITRO GLYCOSYLATION OF PROTEINS AND ENZYMES - The present invention is broadly concerned with new in vitro glycosylation methods that provide rational approaches for producing glycosylated proteins, and the use of glycosylated proteins. In more detail, the present invention comprises methods of glycosylating a starting protein having an amino sidechain with a nucleophilic moiety, comprising the step of reacting the protein with a carbohydrate having an oxazoline moiety on the reducing end thereof, to covalently bond the amino sidechain of the starting protein with the oxazoline moiety, wherein the glycosylated protein substantially retains the structure and function of the starting protein. Target proteins include oxidase, oxidoreductase and dehydrogenase enzymes. The glycosylated proteins advantageously have molecular weights of at least about 7500 Daltons. In a further embodiment, the present invention concerns the use of glycosylated proteins, fabricated by the methods disclosed herein, in the assembly of amperometric biosensors. | 2019-06-20 |
20190185901 | SAMPLE PROCESSING METHOD AND SAMPLE CULTURING METHOD - Provided is a sample processing method that liquefies a medium solution by making a liquid that liquefies the medium solution act on a sample formed by gelating or solidifying the medium solution that is supported by a substrate while an observation subject is included therein, while maintaining a state in which the medium solution is supported by the substrate while the observation subject is included therein. | 2019-06-20 |
20190185902 | BACTERIAL SYSTEMS FOR ANALYZING UBIQUITYLATED POLYPEPTIDES - Bacterial systems for analyzing ubiquitination of proteins is disclosed herein. Kits for analyzing the ubiquitination and methods for carrying out the analysis are also disclosed. | 2019-06-20 |
20190185903 | METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS IN VITRO - The purpose of the present invention is to provide a cell creation method that enables a reduction in cost and time, that is highly safe, and that has great potential for being industrially applied. Provided by the present invention is a method for inducing differentiation of pluripotent cells in vitro into cells having a same phenotype and function as information-presentation cells, the method comprising co-culturing pluripotent cells or a cellular fraction having said pluripotent cells concentrated therein, together with damaged cells or dead cells derived from information-presentation cells, or together with a portion of the damaged cells or dead cells derived from information-presentation cells. | 2019-06-20 |
20190185904 | Tryptophan Oxidase and Use Thereof - A mutated tryptophan oxidase suitable for practical implementation is described herein. Specifically, a mutated tryptophan oxidase wherein at least one amino acid residue of a wild-type tryptophan oxidase is mutated and, as a result, has higher tryptophan oxidase activity and/or stability as compared to the wild-type tryptophan oxidase. The mutated tryptophan oxidase can be derived from a wild-type tryptophan oxidase having at least one of Motifs (2), (3), (5), (7), (9), (11), (13), and (14), and at least one amino acid residue in any of these motifs can have mutation. The mutated tryptophan oxidase also can have a mutation of one or more amino acid residues in an amino acid sequence represented by SEQ ID NO: 2 and a sequence homologous thereto. | 2019-06-20 |
20190185905 | POLYPEPTIDE SUBSTRATE FOR THE DETECTION OF VON WILLIEBRAND FACTOR CLEAVING PROTEASE ADAMTS13 - In a first aspect, there is provided an isolated polypeptide substrate for a disintegrin-like and metallopeptidase with thrombospondin type-1 motif, 13 (ADAMTS13) that is from 45 to 70 amino acids in length and has an amino acid sequence that is substantially similar to part of the von Willebrand factor A2 domain sequence set forth in SEQ ID NO: 2, with one or more of the following modifications: (i) the amino acid corresponding to position 1599 of SEQ ID NO: 2 is mutated from Q to K; (ii) the amino acid corresponding to position 1610 of SEQ ID NO: 2 is mutated from N to C; and (iii) the amino acids corresponding to Q1624 to R1641 of SEQ ID NO: 2 are deleted. In another aspect, there is provided an ADAMTS13 polypeptide substrate that is from 50 to 75 amino acids in length and has an amino acid sequence that is substantially similar to part of the von Willebrand factor A2 domain sequence set forth in SEQ ID NO: 2, with one or more of the following modifications: (i) the amino acid corresponding to position 1599 of SEQ ID NO: 2 is mutated from Q to K; (ii) the amino acid corresponding to position 1610 of SEQ ID NO: 2 is mutated from N to C; (iii) the amino acid corresponding to position 1629 of SEQ ID NO: 2 is mutated from G to E; and (iv) the amino acids corresponding to G1631 to R1641 of SEQ ID NO: 2 are deleted. | 2019-06-20 |
20190185906 | IN SITU VISUALIZATION OF KINASE ACTIVITY - Kinases can be engineered to utilize an ATP analog that is not readily utilized by wild-type kinases by introducing a mutation in the ATP-binding pocket. However, application of this method has been limited by the membrane impermeability of the ATP analog. Provided herein are methods for in situ visualization of substrates of an analog-sensitive kinase, the method comprising a mild fixation step. Also provided herein are kits comprising a fixative, an ATP analog, and an agent for detecting the substrates modified by the ATP analog. | 2019-06-20 |
20190185907 | CONTINUOUS GLUCOSE MONITORING USING AN FAD-DEPENDENT GLUCOSE DEHYDROGENASE - Provided is a method of continuous glucose monitoring (CGM) comprising using an FAD-GDH. The FAD-GDH is capable of retaining initial activity over a certain period of time. Also provided is a method for screening for an FAD-GDH suitable for use in CGM as well as a CGM device comprising an FAD-GDH. | 2019-06-20 |
20190185908 | SYNTHETIC OPLOPHORUS LUCIFERASES WITH ENHANCED LIGHT OUTPUT - A polynucleotide encoding a modified luciferase polypeptide. The modified luciferase polypeptide has at least 60% amino acid sequence identity to a wild-type | 2019-06-20 |
20190185909 | METHODS AND SYSTEMS FOR ANALYZING NUCLEIC ACIDS - The present disclosure provides methods and systems for amplifying and analyzing nucleic acid samples. | 2019-06-20 |
20190185910 | MULTIPLEX PCR METHODS FOR DETECTING GENE FUSIONS, KITS AND COMPOSITIONS - Methods for detecting presence or absence of at least two known gene fusions in isolated genomic DNA comprise subjecting isolated genomic DNA to multiplex PCR. For each known gene fusion, the multiplex PCR employs one or a plurality of forward primers which hybridize to a first gene adjacent its fusion breakpoint location, and one or a plurality of reverse primers which hybridize to a second gene adjacent its fusion breakpoint location. The primers hybridize to the respective gene at consecutive respective positions separated from one another by a plurality of base pairs. Amplified products are detected and respectively represent the presence of a gene fusion. Amplified products may be Sanger sequenced to determine the fusion breakpoints. The identified specific fusion is monitored by a designed fusion PCR. Drug-resistant mutations are detected using multiplex mutation real-time PCR in patient plasma cell-free DNA during targeted therapy, for example, tyrosine kinase inhibitor-targeted therapy. | 2019-06-20 |
20190185911 | MULTIPHASE NUCLEIC ACID AMPLIFICATION - Improved methods for use in nucleic acid amplification, including multiplex amplification, where the amplification is carried out in two or more distinct phases are disclosed. The first phase amplification reaction preferably lacks one or more components required for exponential amplification. The lacking component is subsequently provided in a second, third or further phase(s) of amplification, resulting in a rapid exponential amplification reaction. The multiphase protocol results in faster and more sensitive detection and lower variability at low analyte concentrations. Compositions for carrying out the claimed methods are also disclosed. | 2019-06-20 |
20190185912 | ADAMTS5 Gene Quantification Method, Primer Pair, Combination of Primer Pair and Probe, and Kit - A method for quantifying an ADAMTS5 gene, the method includes: measuring an amount of the ADAMTS5 gene in a sample through a quantitative PCR, wherein a primer consisting of a nucleotide sequence set forth in SEQ ID NO: 2 and a primer consisting of a nucleotide sequence set forth in SEQ ID NO: 3 are used as an amplification primer pair for amplifying a region including the ADAMTS5 gene. | 2019-06-20 |
20190185913 | COMPOSITIONS AND METHODS FOR DETECTION OF NUCLEIC ACID MUTATIONS - The invention provides methods and compositions for detecting a mutation in a target gene in a sample of blood or a fraction thereof, including in certain examples, a fraction that includes circulating tumor DNA. The methods can include a tiling PCR reaction, for example a one-sided multiplex tiling reaction. Virtually any type of mutation can be detected with the methods and compositions. In certain embodiments, gene fusions are detected. Improved PCR methods, especially for performing nested multiplex PCR reactions are provided. | 2019-06-20 |
20190185914 | PROCESS FOR THE AMPLIFICATION OF NUCLEIC ACIDS AND USE OF A KIT FOR CARRYING OUT THE SAME - It is disclosed a method for amplification of nucleic acids in which substantially use is made of the fact that a pre-defined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target sequence-specific activator oligonucleotide. The target sequence-specific activator oligonucleotide causes the separation of re-synthesized complementary primer extension products by means of strand displacement, so that a new primer oligonucleotide can attach to the respective template strand. The thus formed complex of a primer oligonucleotide and a template strand can initiate a new primer extension reaction. The thus formed primer extension products in turn function as templates, so that an exponential amplification reaction results. | 2019-06-20 |
20190185915 | ELECTROCHEMICAL DETECTION OF BACTERIAL AND/OR FUNGAL INFECTIONS - The present disclosure relates to methods and devices for amplifying a plurality of targets in a single PCR run while distinguishing between clinically relevant amplification and amplification from other sources such as from background contamination. The methods and devices further enable discrimination between gram-positive, gram-negative and fungal infections as wells as identify antimicrobial resistance genes. When applying the methods and devices of the invention, the species or genus of an infection(s), and genus of a fungal co-infection(s) or category of bacterial (gram-positive or negative) co-infection(s) are identified. Species identification of co-infections can also be achieved. Further, when applying the methods and devices of the invention, organisms which are likely to be contaminating organisms from a blood draw are identified. | 2019-06-20 |
20190185916 | METHOD FOR ISOLATING TARGET NUCLEIC ACID USING HETERODUPLEX BINDING PROTEINS - The invention includes methods and apparatus for separating mutations, especially rare and unknown mutations, using heteroduplex binding proteins. Nucleic acids may optionally be nicked at or near the mutation in order to promote heteroduplex binding protein recognition and binding. In particular, using the disclosed methods, it is possible to separate heteroduplexed nucleic acid strand pair from homoduplexed nucleic acid strand pairs having similar sequences and being at a much higher concentration. Once the heteroduplexed nucleic acids are isolated and recovered, it is straightforward to analyze the sequences of the heteroduplexed nucleic acids, e.g., using sequencing or hybrid assays. | 2019-06-20 |
20190185917 | CLOSED NUCLEIC ACID STRUCTURES - The invention provides compositions and methods for making closed nucleic acid structures in which one or both strands are continuous. The closed nucleic acid structures can be used as sequencing templates among other applications. | 2019-06-20 |
20190185918 | COUMARIN COMPOUNDS AND THEIR USES AS FLUORESCENT LABELS - The present application relates to new coumarin compounds and their uses as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications. | 2019-06-20 |
20190185919 | Compositions and Methods for Analyzing Modified Nucleotides - Methods and compositions are provided for identifying any of the presence, location and phasing of methylated and/or hydroxymethylated cytosines in nucleic acids including long stretches of DNA. In some embodiments, the method may comprise reacting a first portion (aliquot) of a nucleic acid sample with a dioxygenase and optionally a glucosyltransferase in a reaction mixture containing the nucleic acid followed by a reaction with a cytidine deaminase to detect and optionally map | 2019-06-20 |
20190185920 | METHODS AND SYSTEMS THAT DETECT NUCLEIC-ACID TARGETS - The current document relates generally to the field of nucleic-acid detection and, in particular, to a highly sensitive and specific nucleic-acid-detection method that includes hybridization of a specific nucleic-acid target to a recognition probe, subsequent specific cleavage of the double-stranded target-probe helix at a specific restriction site, and exponential amplification of the enzymatic cleavage accompanied by release of a molecular marker. | 2019-06-20 |
20190185921 | POLYNUCLEOTIDE SEPARATION METHOD - Various method of isolating a modified polynucleotide having the formula A-P-B from a crude mixture of modified polynucleotides also including those having the formulae A-P-A and B-P-B, wherein P is a polynucleotide region and A and B are different modifying moieties or nucleotide sequences are provided. The methods include the steps of (a) reacting the crude mixture concurrently or consecutively with beads of one type capable of binding to moiety A but not B, and beads of a second type capable of binding to moiety B but not A; (b) fractionating the intermediate product based on the properties of each type of bead and of pairs of different types of bead conjoined by A-P-B polynucleotides, such that only or predominantly conjoined pairs of the two different types of bead are retained and (c) optionally, releasing one or both beads from such conjoined pairs such that the polynucleotide may be recovered. | 2019-06-20 |
20190185922 | LUMINOPHORE-LABELED MOLECULES COUPLED WITH PARTICLES FOR MICROARRAY-BASED ASSAYS - A method for labeling target molecules coupled to particles for the detection of the target molecules using a microarray chip, comprises: providing a functionalized microparticle, wherein the microparticle is coated with one or more functional group; providing a modification group on each of the target molecules to be detected to form modified target molecules; contacting the functionalized microparticle with the modified target molecules; coupling a luminophore to the complex between the functionalized microparticle and the modified target molecules, thereby directly or indirectly labeling each modified target molecules with the luminophore. By directly or indirectly labeling the target molecules with the luminophore, the method reduces the cost of fluorescence detection, and avoids PCR inhibition derived from traditional fluorescence labeling molecules. | 2019-06-20 |
20190185923 | CYSTIC FIBROSIS SERUM BIOMARKERS - Systems, methods, devices, and kits are described that can be used to distinguish cystic fibrosis patients from healthy individuals and from lung cancer patients. The systems, methods, devices, and kits utilize one or more serum biomarkers. | 2019-06-20 |
20190185924 | CLOSED LINEAR DNA PRODUCTION - The present invention relates to improved processes for production of closed linear deoxyribonucleic acid (DNA), in particular cell-free enzymatic production of closed linear DNA molecules, preferably using a closed linear DNA as a template for DNA synthesis. The invention further relates to a novel closed linear DNA species, suitable for use as a template in the improved processes for production of closed linear DNA. Further, the invention pertains to the intermediate products of the processes, since this enables the production of larger quantities of closed linear DNA from the template than with methods known in the art. | 2019-06-20 |
20190185925 | GENETIC TEST FOR DETECTING CONGENITAL ADRENAL HYPERPLASIA - A method for amplifying a CYP21A2 gene and/or a CYP21A2 gene chimera from a sample is provided. In some embodiments, the method may comprise amplifying a product from a sample comprising human genomic DNA by PCR using a forward primer that is complementary to a sequence that is duplicated in a bimodular human RCCX locus and a reverse primer that is complementary to a sequence that occurs only once in the bimodular human RCCX locus at a position that is downstream of the CYP21A2 gene. Methods for analyzing the amplification product are also provided. | 2019-06-20 |
20190185926 | MODULAR NUCLEOTIDE COMPOSITIONS AND USES THEREFOR - Nucleic acid compositions, methods of making and using such compositions that comprise modular functional groups that can be configured to provide desired functionality to different nucleotide types through a swappable and preferably non-covalent linkage component. Such compositions are useful in a variety of applications including nucleic acid analyses. | 2019-06-20 |
20190185927 | METHODS FOR FORMING LIPID BILAYERS ON BIOCHIPS - This disclosure provides a biochip comprising a plurality of wells. The biochip includes a membrane that is disposed in or adjacent to an individual well of the plurality of wells. The membrane comprises a nanopore, and the individual well comprises an electrode that detects a signal upon ionic flow through the pore in response to a species passing through or adjacent to the nanopore. The electrode can be a non-sacrificial electrode. A lipid bilayer can be formed over the plurality of wells using a bubble. | 2019-06-20 |
20190185928 | PROSTATE CANCER ASSOCIATED CIRCULATING NUCLEIC ACID BIOMARKERS - The invention provides methods and reagents for diagnosing prostate cancer that are based on the detection of biomarkers in the circulating nucleic acids from a patient to be evaluated. | 2019-06-20 |
20190185929 | SINGLE-CELL NUCLEIC ACIDS FOR HIGH-THROUGHPUT STUDIES - Described herein are cell-based analytic methods, including a method of incorporating nucleic acid sequences into reaction products from a cell population, wherein the nucleic acid sequences are incorporated into the reaction products of each cell individually or in small groups of cells individually. Also described herein is a matrix-type microfluidic device that permits at least two reagents to be delivered separately to each cell or group of cells, as well as primer combinations useful in the method and device. | 2019-06-20 |
20190185930 | METHODS OF PREPARING A SEQUENCING LIBRARY ENRICHED FOR DUPLEX DNA MOLECULES - Methods for preparing sequencing libraries from a DNA-containing test sample, as well as methods for correcting sequencing-derived errors in sequence reads, and methods for identifying rare variants in a test sample, are provided. | 2019-06-20 |
20190185931 | METHODS FOR ANALYZING NUCLEIC ACIDS FROM SINGLE CELLS - Aspects of the present invention include analyzing nucleic acids from single cells using methods that include using tagged polynucleotides containing multiplex identifier sequences. | 2019-06-20 |
20190185932 | METHOD FOR PREPARING LIBRARIES FOR MASSIVELY PARALLEL SEQUENCING BASED ON MOLECULAR BARCODING AND USE OF LIBRARIES PREPARED BY THE METHOD - Provided is a method for preparing libraries for massively parallel sequencing. The method includes: providing two or more double-stranded nucleic acid molecules; ligating adaptors to both ends of each of the nucleic acid molecules; providing a pair of primers for amplifying each nucleic acid molecule wherein each of the paired primers includes i) a 3′-end having a nucleotide sequence complementary to the corresponding adaptor, ii) a 5′-end having a common primer sequence for massively parallel sequencing, and iii) an index sequence located between the 3′-end and the 5′-end, one of the two index sequences is a sequence specific to the corresponding nucleic acid molecule, and the other index sequence is a sequence indexing a sample from which the nucleic acid molecule is derived; and performing amplification using the paired primers to obtain amplification products of the nucleic acid molecules including the molecule-specific sequences and the sample indexing sequences. | 2019-06-20 |
20190185933 | DETECTION AND QUANTIFICATION OF RARE VARIANTS WITH LOW-DEPTH SEQUENCING VIA SELECTIVE ALLELE ENRICHMENT OR DEPLETION - This disclosure describes methods for enabling accurate detection and quantitation of rare alleles within a DNA sample using low-depth sequencing, through the use of allele-specific enrichment and/or depletion hybridization probes. For example, methods are provided for using competitive probes to apply allele-specific enrichment or depletion to amplicons from multiplex PCR on a biological DNA sample. | 2019-06-20 |
20190185934 | METHODS FOR RNA DETECTION AND QUANTIFICATION - The present invention relates to novel nucleic acid molecules, called aptamers, that bind specifically to a small molecule fluorophore and thereby enhance the fluorescence signal of the fluorophore upon exposure to radiation of suitable wavelength. Molecular complexes formed between the novel fluorophores, novel nucleic acid molecules, and their target molecules are described, and the use of multivalent aptamer constructs as fluorescent sensors for target molecules of interest are also described. | 2019-06-20 |
20190185935 | COMPOSITIONS AND METHOD FOR TREATING COMPLEMENT-ASSOCIATED CONDITIONS - The invention provides methods and compositions for treating various degenerative diseases (e.g., AMD) with a factor D inhibitor (e.g., anti-factor D antibody or antigen-binding fragment thereof). Also provided are methods of selecting or identifying patients for treatment with a factor D inhibitor. Methods include the use of prognostic and/or predictive biomarkers. | 2019-06-20 |
20190185936 | METHODS FOR SIMULTANEOUS AMPLIFICATION OF TARGET LOCI - The invention provides methods for simultaneously amplifying multiple nucleic acid regions of interest in one reaction volume as well as methods for selecting a library of primers for use in such amplification methods. The invention also provides library of primers with desirable characteristics, such as minimal formation of amplified primer dimers or other non-target amplicons. | 2019-06-20 |
20190185937 | RNA EDITING AS BIOMARKERS FOR MOOD DISORDERS TEST - The present invention is drawn to a method for in vitro predicting the risk for a patient to present a pathology or to identify whether a patient is at risk to develop a pathology, such as psychiatric disorder, associated to an alteration of A-to-I editing on PDE8A transcripts, from body fluids such as a blood, urine or saliva sample of said patient. The present invention also relates to kits for the implementation of the method. | 2019-06-20 |
20190185938 | DNA METHYLATION BASED PREDICTOR OF MORTALITY - A method for determining the epigenetic age acceleration of an individual comprising measuring a methylation level of a set of methylation markers in genomic DNA of an individual. An epigenetic age of the individual is determined based on the measured methylation level. An epigenetic age of the individual is further determined based on a methylation derived weighted average cell count of naive cytotoxic T cells and exhausted cytotoxic T cells in the individual. The determined epigenetic age is then compared to a chronological age of the individual to determine an epigenetic age acceleration of the individual. Instances wherein the epigenetic age is greater than the chronological age of the individual is an indication of an increased risk of all-cause mortality. | 2019-06-20 |
20190185939 | METHODS AND MATERIALS FOR ASSESSING HOMOLOGOUS RECOMBINATION DEFICIENCY - This document provides methods and materials involved in assessing samples (e.g., cancer cells) for the presence of homologous recombination deficiency (HRD) or an HRD signature. For example, methods and materials for determining whether or not a cell (e.g., a cancer cell) contains an HRD signature are provided. Materials and methods for identifying cells (e.g., cancer cells) having a deficiency in homology directed repair (HDR) as well as materials and methods for identifying cancer patients likely to respond to a particular cancer treatment regimen also are provided. | 2019-06-20 |