25th week of 2014 patent applcation highlights part 55 |
Patent application number | Title | Published |
20140170656 | Methods of Detecting Target Nucleic Acids - The present disclosure relates to methods of identifying target nucleic acids by using coded molecules and its analysis by translocation through a nanopore. Generally, coded molecules are subject to a target polynucleotide dependent modification. The modified coded molecule is detected by isolating the modified coded molecules from the unmodified coded molecules prior to analysis through the nanopore or by detecting a change in the signal pattern of the coded molecule when analyzed through the nanopore. | 2014-06-19 |
20140170657 | Probe for Detection of Polymorphism in EGFR Gene, Amplification Primer, and Use Thereof - A polymorphism-detecting probe, an amplification primer and the use thereof are provided to enable simple and highly reliable determination of different polymorphisms in an EGFR gene. | 2014-06-19 |
20140170658 | MAMMALIAN CYTOKINES; RELATED REAGENTS - Purified genes encoding a cytokine or composite cytokine from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding these molecules are provided. Methods of using said reagents and diagnostic kits are also provided. | 2014-06-19 |
20140170659 | EXPRESSION OF ISOFORM 202 OF ERCC1 FOR PREDICTING RESPONSE TO CANCER CHEMOTHERAPY - An in vitro method for detecting the susceptibility of a tumor cell to a chemotherapy is disclosed. The method includes the step of measuring the expression level of the isoform 202 of the ERCC1 protein. | 2014-06-19 |
20140170660 | METHODS AND COMPOSITIONS FOR PREDICTING UNOBSERVED PHENOTYPES (PUP) - Methods for predicting unobserved phenotypes are provided. In some embodiments, the methods include (a) determining marker effects for a plurality of markers in a genotyped and phenotyped reference population with respect to a phenotype, wherein the reference population includes an F | 2014-06-19 |
20140170661 | NUCLEIC ACID AMPLIFICATION - A method of monitoring amplification of a nucleic acid by providing a nucleic acid and an amplification mixture using the kit of isothermal reagents to a pH sensor or pH indicator, amplifying the nucleic acid using isothermal amplification, and detecting a change in pH due to the amplification using the pH sensor or pH indicator. The kit of reagents comprises a magnesium salt, a quaternary ammonium salt, and an alkali base. | 2014-06-19 |
20140170662 | MUTATIONS IN THE EPIDERMAL GROWTH FACTOR RECEPTOR GENE - The invention relates to a new identified mutation in the epidermal growth factor receptor gene, leading to an amino acidic change which highly correlates with the resistance to a therapy regimen comprising cetuximab and the sensitivity to a therapy regimen comprising panitumumab. The invention includes peptide sequences, primers and probes to detect such a mutation, as well as kits for predicting the response of a subject to a therapy regime comprising cetuximab and/or panitumumab. In particular, the invention is useful in the therapy regimen applicable to metastasic colorectal cancer and to head and neck cancer. | 2014-06-19 |
20140170663 | METHYLATION SIGNATURE FOR REPLICATIVE SENESCENCE OF CELLS IN CULTURE - A method for determining a replicative senescence status of a cell includes determining a methylation status of at least one CpG-dinucleotide within a region of at least one of about 50,000 bp upstream and downstream of at least one CpG-dinucleotide selected from the group consisting of GRM7-CpG-site #1, CASR-CpG-site #1, PRAMEF2-CpG-site #1, SELP-CpG-site #1, CASP14-CpG-site #1, and KRTAP13-3-CpG-site #1. The methylation status of each of the at least one CpG-dinucleotide determined is compared with a reference methylation status of the respective CpG-dinucleotide. | 2014-06-19 |
20140170664 | HEATING MECHANISM FOR DNA AMPLIFICATION, EXTRACTION OR STERILIZATION USING PHOTO-THERMAL NANOPARTICLES - A heating mechanism for use in DNA applications such as DNA amplification, extraction and sterilization is provided. Nanoparticles having photo-thermal properties are put in contact with a reaction mixture and irradiated with an activation light beam to activate these photo-thermal properties, thereby releasing heat. Nanoparticles of several types may be used. Use of the same nanoparticles or of different one to monitor the reaction using a different light beam is also presented. | 2014-06-19 |
20140170665 | SYSTEMS AND METHODS FOR STABILIZING DROPLETS - This disclosure provides systems, devices, and methods for sample preparation and/or analysis comprising a droplet generator having a first channel in fluid communication with a carrier fluid reservoir and a second channel in fluid communication with a sample reservoir. The first channel and second channel may meet at an intersection that receives a sample from the sample reservoir and a carrier fluid from the carrier fluid reservoir and generates one or more droplets that flow along a droplet channel to a droplet reservoir. An energy application member in thermal communication with the intersection, at least a portion of the droplet channel, the sample reservoir and/or the carrier fluid reservoir may provide energy to an individual droplet of the one or more droplets, such as at the intersection and/or as the droplet moves along the droplet channel to the droplet reservoir. | 2014-06-19 |
20140170666 | METHODS, COMPOSITIONS AND KITS FOR THE IMPROVED DETECTION OF SMALL RNA MOLECULES - The present invention provides compositions, methods and kits for use in the detection of small RNA sequences, which allow for rapid and robust amplification and detection. The methods provide improved sensitivity and efficiency in the amplification-based detection of small RNA sequences by incorporating one or more base-modified duplex-stabilizing dNTPs during reverse transcription and/or amplification. | 2014-06-19 |
20140170667 | METHODS FOR AMPLIFYING NUCLEIC ACID FROM A TARGET - The invention generally relates to methods for amplifying nucleic acid from a target. In certain aspects, methods of the invention involve obtaining a sample including a target, conducting an assay that isolates the target from the sample, lysing the target to release the nucleic acid, and amplifying the released nucleic acid such that the amplifying occurs in the presence of debris from the lysing step. | 2014-06-19 |
20140170668 | MONOCLONAL AND OLIGOCLONAL ANTI-EGFR ANTIBODIES FOR USE IN THE TREATMENT OF TUMORS EXPRESSING PREDOMINANTLY HIGH AFFINITY EGFR LIGANDS OR TUMORS EXPRESSING PREDOMINANTLY LOW AFFINITY EGFR LIGANDS - Disclosed are pharmaceutical preparations for, and methods for determining, appropriate and effective treatment with therapeutic agents comprising a single species of anti-EGFR monoclonal antibody or therapeutic agents comprising a plurality of species of such antibodies, as well as kits useful for making such determinations. | 2014-06-19 |
20140170669 | DEVICES FOR TARGET DETECTION AND METHODS OF USE THEREOF - The invention generally relates to devices for target detection and methods of use thereof. | 2014-06-19 |
20140170670 | Methods and Compositions for Antibody Therapy - Methods and materials are provided for determining the responsiveness of a subject to a therapy, such as an antibody therapy and for selecting and/or for treating a subject based on an FcγRIIA polymorphism, or an FcγRIIIA polymorphism, or both an FcγRIIA polymorphism and an FcγRIIIA polymorphism, including where the treatment is an therapy, such as rituximab. Methods and materials are also provided for designing, making, screening, testing and/or administering antibodies as well as for optimizing antibody therapies based upon a subject's FcγRIIA polymorphism, or FcγRIIIA polymorphism, or both the FcγRIIA polymorphism and the FcγRIIIA polymorphism. | 2014-06-19 |
20140170671 | APPARATUS AND METHODS FOR THREE-DIMENSIONAL TISSUE MEASUREMENTS BASED ON CONTROLLED MEDIA FLOW - An apparatus including a plurality of wells for conducting analysis of three-dimensional cell samples (e.g., tissue samples) and methods for experimenting with a three-dimensional sample. A removable insert for use with the apparatus enables plunger-driven perfusion of the three-dimensional sample. | 2014-06-19 |
20140170672 | Portable Clinical Analysis System for Immunometric Measurement - The present invention covers the integration and utility of accelerometer features into a clinical analysis system. For example, measurement of dynamic acceleration and orientation of a blood-testing instrument with respect to Earth's gravitational field may be used to determine reliability of a test procedure and optionally to provide corrective elements thereof. | 2014-06-19 |
20140170673 | UNITARY CARTRIDGE FOR PARTICLE PROCESSING - A single disposable cartridge for performing a process on a particle, such as particle sorting, encapsulates all fluid contact surfaces in the cartridge for use with microfluidic particle processing technology. The cartridge interfaces with an operating system for effecting particle processing. The encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The cartridge may employ any suitable technique for processing particles. | 2014-06-19 |
20140170674 | Membraine-Based Assay Devices Utilizing Time-Resolved Up-Converting Luminescence - This invention discloses an immunochromatographic assay device to detect and quantify analytes. The device utilizes up-converting luminescent probes to detect time-resolved luminescence signals. Because the unconverting luminescent probes can have relatively long emission lifetime, background interference from sample autofluorescence and light scattering from excitation source can be easily eliminated through delayed luminescence detection. Furthermore, the up-converting luminescent probes can be excited by near Infrared (IR) or IR light sources. In comparing with UV and visible lights, the near IR and IR lights can penetrate deeper into sample matrices and more effectively excite the probes, but not the sample matrices, resulting in less background and higher detection sensitivity. A simple and low cost reader can be designed to measure the delayed up-converting luminescence of long lifetime that does not use expensive optical filters and mirrors. | 2014-06-19 |
20140170675 | PROTEIN DETECTOR BASED ON MOLECULAR CONTROLLED SEMICONDUCTOR RESISTOR - The invention provides a semiconductor device for the detection of an active site-containing protein or a ligand thereof in a solution, said device comprising at least one insulating or semi-insulating layer; at least one conducting semiconductor layer, two conducting pads on top of the upper layer making electrical contact with said at least one conducting semi-conductor layer, such that electrical current can flow between them at a finite distance from the surface of the device; a protective molecular layer fabricated on top of said upper layer and protecting said layer from corrosion; and said ligand or active site-containing protein linked to said protective molecular layer. Exposure of said ligand or active site-containing protein to a solution containing said active site-containing protein or ligand, respectively, causes a current change through the device when a constant electric potential is applied between the two conducting pads. The semi-conductor device can be seen as a molecularly controlled semiconductor resistor (MOCSER) protein sensor based on doped and undoped GaAs stack structure. The GaAs is protected against etching in aqueous environments by the protective molecular layer. | 2014-06-19 |
20140170676 | RBM3 in Bladder Cancer - The present disclosure relates, in one of its aspects, to a method for determining whether a mammalian subject having a bladder cancer belongs to a first or a second group, wherein the prognosis of subjects of the first group is better than the prognosis of subjects of the second group. The method comprises the steps of: a) evaluating an amount of RBM3 in at least part of a sample earlier obtained from the subject and determining a sample value corresponding to the evaluated amount; b) comparing said sample value with a predetermined reference value; and if said sample value is higher than said reference value, c1) concluding that the subject belongs to the first group; and if said sample value is lower than or equal to said reference value, c2) concluding that the subject belongs to the second group. | 2014-06-19 |
20140170677 | USE OF THE ANTIBODY I-3859 FOR THE DETECTION AND DIAGNOSIS OF CANCER - The present invention relates to the use of a novel, isolated anti-CXCR4 antibody in the diagnosis of cancer. In particular, methods for diagnosing and/or prognosing an oncogenic disorder associated with CXCR4 expression, are disclosed. | 2014-06-19 |
20140170678 | KITS, COMPOSITIONS AND METHODS FOR DETECTING A BIOLOGICAL CONDITION - The present invention provides kits, apparatus and methods for determining a biological condition in a mammalian subject, the method includes incubating a specimen from a patient with at least one composition in a kit for a predetermined period of time to form at least one reaction product, when the subject has said biological condition, and receiving an indication of the at least one reaction product responsive to at least one reporter element in the kit thereby providing the indication of the biological condition in the subject. | 2014-06-19 |
20140170679 | METHOD AND SYSTEM FOR CELL DETECTION AND ANALYSIS - The present invention is a method and a system of cell detection and analysis. The present invention may incorporate at least an optical source, a fluidic chip and a detection module. Cells may be caused to flow within the fluidic chip and specifically past a detection window section accessible by the optical source. The flowing cells may be identified and/or analyzed. The detection module may specifically count the cells of interest as they flow past the detection window section of the chip. The detection module may further be operable to generate or otherwise capture images of the cells as they flow past the window and to use these images collectively for the purpose of analyzing the cells. The present invention may be portable and operable in remote locations. | 2014-06-19 |
20140170680 | SYSTEMS, COMPOSITIONS AND METHODS FOR DETECTING A BIOLOGICAL CONDITION - Determining a biological condition in a mammal by using a cartridge, having fluidic open channels, sealable after receiving a fluid specimen, passing any of the specimen through any of the channels, contacting any reagent stored in a chamber with the specimen in a reaction chamber inducing a reaction and forming a reaction product, a mechanical controller including first urging means applying a force externally onto the chamber to release the reagent, second urging means applying a removable force onto the channels thereby inducing fluidic movement in a first direction in the channels and upon removal of the force causing fluidic movement in an opposite direction, alignment means aligning a reading channel on the cartridge for a detection to take place, an optical reader detecting the reaction product in the reading channel, and a processor receiving data from the optical reader and processing the data to determine the biological condition. | 2014-06-19 |
20140170681 | NOVEL ANTI-CXCR4 ANTIBODY AND ITS USE FOR THE DETECTION AND DIAGNOSIS OF CANCER - The present invention provides a novel, isolated anti-CXCR4 antibody for use in the diagnosis of cancer. In particular, the antibody of the invention recognizes monomeric and homodimeric CXCR4, but not heterodimeric CXCR4 | 2014-06-19 |
20140170682 | BIOMARKERS OF IONIZING RADIATION - The present invention provides novel radiation associated markers. The radiation associated markers may be one or more of albumin, LTGF-β, or any protein or peptide listed in any one of Tables 1, 2, 3, 4, 5, and 6 provided herein. The present invention also provides methods of assessing exposure to ionizing radiation by determining the presence of one or more radiation associated markers. The methods may optionally include quantifying one or more of the radiation associated markers. The methods may further include comparing the amount of one or more radiation associated markers in the sample determined to be present in the sample with either (i) the amount determined for temporally matched, normal samples or (ii) the amount determined for samples obtained from individuals or subjects that have not been exposed to an elevated level of ionizing radiation. | 2014-06-19 |
20140170683 | CITRULLINATED PEPTIDES FOR DIAGNOSING AND PROGNOSING RHEUMATOID ARTHRITIS - The present invention provides novel citrullinated peptides, their use in methods for aiding, assisting, improving, or facilitating the diagnosis or prognosis of rheumatic diseases such as rheumatoid arthritis (RA), and methods for identifying novel citrullinated peptides that are immunoreactive with anti-citrullinated protein antibodies (ACPAs). The present invention also provides methods for detecting rheumatoid factor (RF) using novel RF detection reagents as a means to aid, assist, improve, or facilitate the diagnosis or prognosis of rheumatic diseases such as RA. Kits comprising at least one of the novel citrullinated peptides and/or RF detection reagents of the present invention are also provided. | 2014-06-19 |
20140170684 | Methods, Devices, Kits and Compositions for Detecting Roundworm - Methods, devices, kits and compositions for detecting the presence or absence of roundworm in a fecal sample are disclosed herein. The methods, devices, kits and compositions of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm. Confirmation of the presence or absence of roundworm in the mammal may be made, for example, for the purpose of selecting an optimal course of treating the mammal and/or for the purpose of determining whether the mammal has been rid of the infection after treatment has been initiated. | 2014-06-19 |
20140170685 | BIOMARKER OF ASBESTOS EXPOSURE AND MESOTHELIOMA - Several embodiments provided herein relate to methods of diagnosing asbestos exposure or mesothelioma. Several embodiments also relate to methods of differentiating whether a tumor of the lung is lung cancer or mesothelioma. | 2014-06-19 |
20140170686 | MUTANT LUCIFERASE - An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from | 2014-06-19 |
20140170687 | MUTANT LUCIFERASE - An isolated recombinant luciferase having luciferase activity. The recombinant luciferase has an amino acid sequence which differs from the wild-type luciferase from | 2014-06-19 |
20140170688 | RAPID, LOW-SAMPLE-VOLUME CHOLESTEROL AND TRIGLYCERIDE ASSAYS - Reagents, assays, methods, kits, devices, and systems for rapid measurement of cholesterol and cholesterol sub-fractions from a blood sample are provided. Total cholesterol, low density lipoprotein cholesterol, and high density lipoprotein cholesterol can be measured in a single assay using kinetic measurements, under conditions in which cholesterol sub-species are converted to a detectable product at distinct rates. The detectable product is measured at different times after assay initiation. A lipase, cholesterol esterase, cholesterol oxidase and a peroxidase may be used together to produce colored product in amounts directly proportional to the quantity of cholesterol converted. Methods for calculating very-low density lipoprotein cholesterol levels by further including triglyceride measurements are disclosed. Assays may be performed in a single reaction mixture, allowing more accurate and precise cholesterol determinations, including ratios of cholesterol sub-fractions to total cholesterol, at less expense, than would be expected by performing several different assays in different reaction mixtures. | 2014-06-19 |
20140170689 | Modified Diguanylate Cyclase-Phosphodiesterase and Method for Enzymatic Production of Cyclic-diGMP - The present invention provides a recombinant polypeptide comprising a first portion and a second portion, wherein the sequence of the first portion is fully identical to amino acids 1 to 248 of the sequence set forth as SEQ ID NO:1 and the sequence of the second portion is other than amino acids 249 to 511 of the sequence set forth as SEQ ID NO:1. | 2014-06-19 |
20140170690 | RADIATION MICRODOSIMETERS CORRELATED WITH BIOLOGICAL CELLS AND CELL COMPONENTS - One feature pertains to a radiation dosimeter comprising a microdosimeter cell array that includes a first microdosimeter cell having a first semiconductor volume configured to generate a first current in response to incident radiation. The first semiconductor volume may have at least one of a first size, a first shape, a first semiconductor type, and/or a first semiconductor doping type and concentration that is associated with a first biological cell type or a first biological cell component type. The dosimeter may further comprise a processing circuit communicatively coupled to the microdosimeter cell array and configured to generate a signal based on the first current. The signal generated may be indicative of an amount of radiation absorbed by the microdosimeter cell array. A display may be utilized by the dosimeter to show a radiation level reading based on the signal generated. | 2014-06-19 |
20140170691 | System for Conducting the Identification of Bacteria in Biological Samples - The present invention relates to a system for conducting the identification and quantification of micro-organisms, e.g., bacteria in biological samples. More particularly, the invention relates to a system comprising a disposable cartridge and an optical cup or cuvette having a tapered surface; an optics system including an optical reader and a thermal controller; an optical analyzer; a cooling system; and an improved spectrometer. The system may utilize the disposable cartridge in the sample processor and the optical cup or cuvette in the optical analyzer. | 2014-06-19 |
20140170692 | QUANTUM NANOSTRUCTURES, COMPOSITIONS THEREOF, AND METHODS OF MAKING AND USING SAME - Provided are methods for making quantum nanostructures based on use of a combination of nucleation and growth precursors. The methods can be used to provide quantum nanostructures of a selected size. Also provided are quantum nanostructures, compositions comprising the quantum nanostructures, and uses of the quantum nanostructures. The quantum nanostructures can be used, for example, in imaging applications. | 2014-06-19 |
20140170693 | COMPOSITIONS AND METHODS FOR CULTURING CELLS FROM NORMAL HUMAN TUBO-OVARIAN EPITHELIUM AND HUMAN TUBO-OVARIAN TUMORS - Described herein are cell culture media, kits and methods for preparing cell culture media, and methods for culturing cells, for example, cells of the female reproductive tract, and tumor cells. | 2014-06-19 |
20140170694 | Spatial Orientation Determination In Portable Clinical Analysis Systems - The present invention covers the integration and utility of accelerometer features into a clinical analysis system. For example, measurement of dynamic acceleration and orientation of a blood-testing instrument with respect to Earth's gravitational field may be used to determine reliability of a test procedure and optionally to provide corrective elements thereof. | 2014-06-19 |
20140170695 | Biological cell nanocavity probes - An optical fiber is combined with a photonic crystal structure (PCS) that is optically coupled to the optical fiber. The fiber has an exposed fiber surface, and the PCS is affixed to the optical fiber and disposed on or in proximity to the exposed fiber surface. The PCS includes an elongate probe member configured for biological probing. The elongate probe member includes an optical resonant cavity. In an experiment, this was accomplished using an optical fiber tip with a semiconductor template attached to its side face. The semiconductor structure had a thin, needle-like tip (including a nanobeam cavity) which can be suitably inserted inside (or broken off inside) a biological cell without causing cytotoxicity. | 2014-06-19 |
20140170696 | FLOW CYTOMETER REMOTE MONITORING SYSTEM - Generally, a computer implemented remote monitoring system which generates a viewable reduced byte data representation for each one of a plurality of analyzed instrument signals. Specifically, a flow cytometer remote monitoring system which generates a viewable reduced byte data representation for each one of a plurality analyzed flow cytometer signals. | 2014-06-19 |
20140170697 | FLOW CELL - A flow cell for particle processing such as particle sorting, may be operatively engaged to a particle processing apparatus. The fluid contact surfaces of the flow cell may be fully enclosed. Further, the flow cell may encapsulate all fluid contact surfaces in the particle processing apparatus. The enclosing or encapsulation of the fluid contact surfaces insures, improves or promotes operator isolation and/or product isolation. The flow cell may employ any suitable technique for processing particles. The flow cell may be disposable and suitable for use in droplet sorting. The flow cell may include an operatively sealed sort chamber having a particle stream focusing region, an orifice, an interrogation zone and a sorting region. | 2014-06-19 |
20140170698 | Microbead Kit and Method for Quantitative Calibration and Performance Monitoring of a Fluorescence Instrument - Fluorescently labeled microsphere calibration and quality control particles for use in establishing standardized fluorescence detector setup, monitoring daily QC of flow cytometers and quantitative calibration of the fluorescence detectors in traceable units of measure (molecules equivalent soluble fluorescence; MESF). | 2014-06-19 |
20140170699 | TEST FOR DETECTING SPOILAGE IN A FLEXIBLE PACKET - An apparatus for detecting spoilage of the contents of a packet is disclosed. The apparatus includes a pair of holding panels ( | 2014-06-19 |
20140170700 | Method For Producing Carotenoids Each Having 50 Carbon Atoms - The present invention provides a method of producing a carotenoid having 50 carbon atoms which comprises culturing, in a medium, a cell transformed with a mutant phytoene desaturase gene and obtaining the carotenoid having 50 carbon atoms from the culture. The mutant phytoene desaturase gene has an introduced mutation to encode a mutant phytoene desaturase having an enhanced activity to desaturate a carotenoid backbone compound of 50 carbon atoms. | 2014-06-19 |
20140170701 | METHOD FOR PRODUCING A RECOMBINANT PROTEIN OF INTEREST - Disclosed is a method for producing a recombinant protein of interest which is characterised by the following steps:
| 2014-06-19 |
20140170702 | METHOD FOR PRODUCING A RECOMBINANT PROTEIN OF INTEREST - Disclosed is a method for producing a recombinant protein of interest, the method being characterised in by the following steps:
| 2014-06-19 |
20140170703 | RECOMBINANT MICROORGANISM - A recombinant microorganism obtained by transferring, into a host microorganism capable of producing protein or polypeptide with increased productivity, a gene encoding a protein or polypeptide, and a method for producing a protein or polypeptide by use of the recombinant microorganism. The recombinant microorganism is prepared by transferring, to a mutant strain of microorganism from which any of | 2014-06-19 |
20140170704 | Production of Carrier-Peptide Conjugates Using Chemically Reactive Unnatural Amino Acids - Provided are methods of making carrier polypeptide that include incorporating a first unnatural amino acid into a carrier polypeptide variant, incorporating a second unnatural amino acid into a target polypeptide variant, and reacting the first and second unnatural amino acids to produce the conjugate. Conjugates produced using the provided methods are also provided. In addition, orthogonal translation systems in methylotrophic yeast and methods of using these systems to produce carrier and target polypeptide variants comprising unnatural amino acids are provided. | 2014-06-19 |
20140170705 | METHODS OF GENERATING LIBRARIES AND USES THEREOF - This invention relates to methods for the generation of humanized antibodies, particularly a humanized antibody heavy chain protein and a humanized antibody light chain protein. The method comprises using cells that express or can be induced to express Activation Induced Cytidine Deaminase (AID). | 2014-06-19 |
20140170706 | LOW-MASS SAMPLE BLOCK WITH RAPID RESPONSE TO TEMPERATURE CHANGE - A sample block for use in the polymerase chain reaction, DNA sequencing, and other procedures that involve the performance of simultaneous reactions in multiple samples with temperature control by heating or cooling elements contacting the bottom surface of the block is improved by the inclusion of hollows in the block that are positioned to decrease the mass of the block in the immediate vicinity of the wells. | 2014-06-19 |
20140170707 | METHOD AND APPARATUS FOR AMPLIFICATION OF NUCLEIC ACID SEQUENCES BY USING THERMAL CONVECTION - The present invention provides a nucleic acid sequence amplification method and apparatuses thereof that are simple in the design and easy to miniaturize and integrate into complex apparatuses, with capability of using DNA polymerases that are not thermostable. In the present invention, a plurality of heat sources are combined to supply or remove heat from specific regions of the sample such that a specific spatial temperature distribution is maintained inside the sample by locating a relatively high temperature region lower in height than a relatively low temperature region. | 2014-06-19 |
20140170708 | COMPOSITIONS AND METHODS FOR CREATING ALTERED AND IMPROVED CELLS AND ORGANISMS - The present invention provides compositions comprising randomized in-frame fusion polynucleotides and methods for introducing them into a host organism to identify desirable phenotypic changes that disrupt or alter existing genetic or biochemical mechanisms or pathways, thus creating novel characteristics of the transformed organism. Methods for using the compositions for increasing diversity within populations of organisms are also presented. | 2014-06-19 |
20140170709 | VECTOR FOR GENE THERAPY - The present invention provides a retroviral vector containing a transcription unit comprising a transcription regulatory sequence and a gene encoding a polypeptide having single-stranded RNA-specific endoribonuclease activity which is placed so that its expression can be controlled by the regulatory sequence, wherein the unit is placed so that the direction of the transcription of mRNA from the unit is opposite to the direction of transcription of the RNA genome of the retroviral vector. By using the vector constructed as described above, viral supernatant showing high gene transfer efficiency can be prepared. The retroviral vector of the present invention is useful for the treatment and/or prevention of cancers and virus infections. | 2014-06-19 |
20140170710 | METHODS AND COMPOSITIONS FOR SEAMLESS CLONING OF NUCLEIC ACID MOLECULES - The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention. | 2014-06-19 |
20140170711 | NUCLEOTIDE ANALOGS AND PROCESS FOR MAKING SAME ENZYME - A process for making a nucleotide analog includes combining a first substrate that includes a linker and a base with a second substrate to form a substrate composition. An enzyme contacts the substrate composition and catalyzes formation of the nucleotide analog from the first substrate and the second substrate. Additionally, a composition includes the first substrate, second substrate, the enzyme, the nucleotide analog, and optional additives. | 2014-06-19 |
20140170712 | Mashing Process - The present invention relates to a mashing and filtration step in a brewing process and to a composition useful in the mashing and filtration step of a brewing process. | 2014-06-19 |
20140170713 | BIOMASS FRACTIONATION PROCESSES, APPARATUS, AND PRODUCTS PRODUCED THEREFROM - Aspects of the AVAP® and Green Power+® technologies may be integrated, as disclosed herein. The present invention, in some variations, couples a first step of steam or hot-water extraction of biomass, with fractionation of the resulting solids using sulfur dioxide (or other acid), an alcohol (or other solvent), and water. In other variations, a first step of fractionation with sulfur dioxide (or other acid), an alcohol (or other solvent), and water is followed by treatment with steam or hot water to reduce hemicellulose content of the final solids. Some embodiments provide cellulose materials with low lignin and low hemicellulose content. Such cellulose materials are useful for making glucose by hydrolysis, as a pulp product, or as purified cellulose for making cellulose derivatives. | 2014-06-19 |
20140170714 | POST PROCESS PURIFICATION FOR GAMMA-BUTYROLACTONE PRODUCTION - Post purification processes and methods for making pure biobased gamma-butyrolactone from renewable carbon resources comprising filtration and/or distillation and/or peroxide treatment are described herein. | 2014-06-19 |
20140170715 | Polypeptides Having Peroxygenase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having peroxygenase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2014-06-19 |
20140170716 | Renewable Diesel and Jet Fuel from Microbial Sources - The invention provides methods of manufacturing alkanes from triglyceride oils produced through fermentation of oil-bearing microbes. The processes provided herein can utilize a variety of carbohydrate feedstocks including cane bagasse, sugar beet pulp, corn stover, glycerol, corn starch, sorghum, molasses, waste glycerol, and other renewable materials. These processes further comprise hydrotreating, hydrocracking, isomerization, distillation, and other petrochemical processes for use with oil-bearing microbes and products derived therefrom to manufacture fuels. Particular embodiments include the manufacture of ASTM D975 and ASTM D1655 compliant fuels. Genetically engineered microbes provided herein can be used in the manufacture of renewable diesel and renewable jet fuel. | 2014-06-19 |
20140170717 | Ester Synthesis - The present invention relates to a process for preparing esters from fatty alcohols, in which fatty alcohols and fatty acids are reacted in the presence of an enzyme at a temperature in the range of 30 to 50° C., the water which forms is removed and the reaction is completed under reduced pressure at a temperature of 50 to 80° C. | 2014-06-19 |
20140170718 | MULTIMERIC OXIDOREDUCTASES - The present invention concerns multimeric oxidoreductase complexes which function in the enzymatic conversion of a carbon substrate, said complexes having a dehydrogenase subunit and a cytochrome C subunit. The invention further relates to polynucleotides coding for the multimeric complexes and methods of use thereof. | 2014-06-19 |
20140170719 | MICROORGANISM AND METHOD FOR LACTIC ACID PRODUCTION - The present invention relates generally to compositions and methods for producing lactic acid using a lactic acid producing microorganism. More specifically, the present invention relates to methods for producing lactic acid with high yield, high concentration and high volumetric productivity through biological fermentation by | 2014-06-19 |
20140170720 | METHOD FOR PRODUCING RETINOID FROM MICROORGANISM - The present invention relates to a method for producing retinoid from a microorganism, and more specifically, to a method for effectively obtaining retinoid, which lacks stability, from a microorganism by cultivating the microorganism capable of producing retinoid in a medium containing a lipophilic substance, and separating retinoid from the lipophilic substance. | 2014-06-19 |
20140170721 | METHODS FOR PRODUCING SUGARS FROM BIOMASS - The present invention provides methods for producing a mixture of sugars from a solid biomass feedstock using a counter current extraction apparatus and a dilute acidic solution. | 2014-06-19 |
20140170722 | METHOD FOR PRODUCING ISOBUTANOL AND RECOMBINANT MICROORGANISM CAPABLE OF PRODUCING ISOBUTANOL - This invention is intended to produce isobutanol with excellent productivity via a fermentation process. A reaction in which NADP-dependent isocitrate dehydrogenase generates NADPH from NADP is used as a source of NADPH for the reaction of converting 2-acetolactate into 2,3-dihydroxy-isovalerate in the isobutanol biosynthesis pathway. | 2014-06-19 |
20140170723 | Sequential Fermentation of Hydrolsate and Solids from a Dilute Acid Hydrolysis of Biomass to Produce Fermentation Products - A method of producing renewable material comprising (a) converting biologically a hemicellulose-derived material to form a first mixture comprising a first renewable material, and (b) convening a substantial amount of a material comprising cellulose and lignin in the presence of at least a portion of the first mixture to form a second mixture comprising second renewable material. | 2014-06-19 |
20140170724 | REPLICATING EXPRESSION VECTOR AND METHODS - This disclosure provides a shuttle vector for transferring genetic material between | 2014-06-19 |
20140170725 | PROCESS AND APPARATUS FOR CAPTURING GASEOUS AMMONIA - A method and system for collecting gaseous nitrogen compounds into an aqueous solution are provided. The method enables the combination of gaseous sulfur and nitrogen compounds in the aqueous solution to generate ammonium compound components, to include ammonium sulfate. Sulfur may be pressure injected into the solution as gaseous sulfur dioxide. Optionally, carbon may be introduced into the solution as gaseous carbon dioxide. The sulfur may be earlier sourced by a burning of a sulfurous solid. The pH of the solution may be monitored and the introduction of ammonia, carbon and/or sulfur may be halted or constrained while the pH of the solution is measured outside of specified range. The solution may be allowed to age to permit a mix of compounds of ammonium carbonate, ammonium bicarbonate and ammonium carbomate to restabilize and thereby encourage a renewed surge of ammonium sulfate generation. | 2014-06-19 |
20140170726 | PHOTOBIOREACTOR - In the present invention, a photobioreactor and process for producing and harvesting microalgae involves a vessel for cultivating microalgae that is at least partially transparent to admit light into the vessel. At least a portion of the transparent part of the vessel is coated with a transparent conductive oxide (TCO) layer. The TCO layer is transparent to visible light necessary for algae growth, but is opaque to infrared light thereby reducing thermal heating load in the photobioreactor. The TCO layer also acts as an electrode, which when combined with a counter-electrode can provide a potential difference across at least a portion of the interior of the vessel between the TCO layer and the counter-electrode. The electrode arrangement can be utilized in an electrochemical process (e.g. electrodeposition and/or electroflotation) to dewater and harvest the microalgae in the same apparatus as the microalgae was cultivated. | 2014-06-19 |
20140170727 | AFFINITY MEDIUM USING FIXED WHOLE CELLS - Separation media comprising a support and crosslinked whole cells fixed to the support. The separation media are useful in affinity columns for the separation of the antibodies from a solution, particularly for the separation of polyclonal antibodies that have been raised against the same type of whole cells. In an embodiment, the whole cells are whole bacterial cells. | 2014-06-19 |
20140170728 | Factor IX: Remodeling and Glycoconjugation of Factor IX - The invention includes methods and compositions for remodeling a peptide molecule, including the addition or deletion of one or more glycosyl groups to a peptide, and/or the addition of a modifying group to a peptide. | 2014-06-19 |
20140170729 | SSO7-POLYMERASE CONJUGATES WITH DECREASED NON-SPECIFIC ACTIVITY - Improved Sso7-polymerase conjugate proteins are provided. | 2014-06-19 |
20140170730 | DNA POLYMERASES WITH IMPROVED ACTIVITY - Disclosed are DNA polymerases having increased reverse transcriptase efficiency relative to a corresponding, unmodified polymerase. The polymerases are useful in a variety of disclosed primer extension methods. Also disclosed are related compositions, including recombinant nucleic acids, vectors, and host cells, which are useful, e.g., for production of the DNA polymerases. | 2014-06-19 |
20140170731 | METHODS, SYSTEMS AND COMPOSITIONS RELATED TO MICROBIAL BIO-PRODUCTION OF BUTANOL AND/OR ISOBUTANOL - Embodiments herein generally relate to methods, compositions, systems and uses for enabling bio-production of or increasing bio-production of alcohol molecules by microorganisms. Certain embodiments relate to compositions and methods enabling or increasing the bio-production of 4-carbon alcohol molecules by bacteria. In some embodiments, compositions and methods relate to introducing isobutyryl-CoA isomerase to a culture of microorganisms to enable or increase the bio-production of four-carbon alcohols. Variations of biosynthesis pathways for microbial bio-production of butanol and/or isobutanol are provided. | 2014-06-19 |
20140170732 | Butanol Dehydrogenase Enzyme from the Bacterium Achromobacter xylosoxidans - From a bacterial strain isolated from an environmental sample, after enrichment in medium containing 1-butanol as the carbon source, a new enzyme with butanol dehydrogenase activity was identified. The enzyme can convert butyraldehyde to 1-butanol, isobutyraldehyde to isobutanol, as well as 2-butanone to 2-butanol and thus is useful for biosynthesis of butanol in recombinant microbial hosts producing these substrates. The encoding gene, named sadB, was isolated from the strain identified as an isolate of | 2014-06-19 |
20140170733 | ALGAE CULTIVATION METHOD AND ALGAE CULTIVATION EQUIPMENT - As a simple and convenient method for promoting the proliferation of algae, a method for cultivating the algae while conducting a procedure S | 2014-06-19 |
20140170734 | NUCLEIC ACID TEST APPARATUS - The nucleic acid amplification device is configured to amplify the nucleic acid in the reaction solution obtained by mixing a specimen and a reagent and includes a plurality of temperature regulation block configured to hold at least a reaction container provided on a holder base. The order and timings in which the reaction containers are continuously installed on the temperature regulation blocks and in which the temperature regulations of the temperature regulation blocks are started are controlled to minimize the temperature gradient in the holder base. | 2014-06-19 |
20140170735 | SYSTEMS AND METHODS FOR MULTI-ANALYSIS - Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample. | 2014-06-19 |
20140170736 | METHODS AND COMPOSITIONS FOR USING OILS FOR ANALYSIS AND DETECTION OF MOLECULES - Oil compositions and methods for use in droplet formation or in spacing of droplets are disclosed. The oil compositions may include a combination, such as a silicone oil and a fluorine-containing compound. The droplets can contain analytes (e.g., nucleic acids) and may be used for molecular reactions (e.g., digital PCR) and detection. | 2014-06-19 |
20140170737 | STACKABLE MICRO-FLUIDIC CELLS - An assay assembly that includes an assay bar having a plurality flow cells is disclosed. Each of the flow cells includes an inlet, an outlet, and an inside surface defining an inner volume. The outlet includes a valve that is configured to retain liquid within the inner volume of the flow cell. Each assay bar is configured to be reversibly stacked upon another assay bar, such that the flow cells of the stacked assay bars are in fluid communication with each other. This way, the outlet of a first flow cell of a first assay bar is in fluid communication with the inlet of a second flow cell of a second assay bar. The assay assembly may include a multitude of assay bars to form a composite assay assembly, with the flow cells of the stacked assay bars being in fluid communication with each other. | 2014-06-19 |
20140170738 | CONCENTRATION MEASURING DEVICE - A concentration measuring device with which the concentration measurement of a test substance can be facilitated as compared to a conventional technique is provided. The concentration measuring device includes: first to third pipes together forming a circulation path through which a gas can circulate; a pump for flowing the gas in a circulating direction in the circulation path; a sample disposed in the circulation path; and a concentration measuring mechanism for measuring a concentration of a target substance from the sample in the circulation path. The concentration measuring mechanism includes, for example, a sample heater, a catalyst, a catalyst heater, a detection chamber, a concentration sensor, and a control unit. | 2014-06-19 |
20140170739 | INLINE SAMPLE FILTER FOR A FLOW CYTOMETER - An inline sample filter for a flow cytometer. | 2014-06-19 |
20140170740 | Optics Cup With Curved Bottom - The present invention relates to a system for conducting the identification and quantification of micro-organisms, e.g., bacteria, in biological samples. More particularly, the invention relates to a system comprising a disposable cartridge and an optics cup or cuvette having a tapered surface; wherein the walls are angled to allow for better coating and better striations of the light. The system may utilize the disposable cartridge in the sample processor and the optics cup or cuvette in the optical analyzer, wherein the optics cup also has a floor in the shape of an inverted arch. | 2014-06-19 |
20140170741 | HEPATIC FIBROSIS DETECTION APPARATUS AND SYSTEM - A hepatic fibrosis detection apparatus and system include an input device, for receiving age and serum biochemical variables, the serum biochemical variables at least including blood platelet, hyaluronic acid, serum direct bilirubin, pro-thrombin time, serum glutamic pyruvic transaminase and serum glutamic oxaloacetic transaminase; a classifier, for performing hepatic fibrosis staging according to the age and serum biochemical variables received by the input device and transient elastography imaging data; and an output device, for outputting a result of the hepatic fibrosis staging of the classifier. The system provides various benefits such as non-invasiveness, high practicability, simple method, low cost and high safety. | 2014-06-19 |
20140170742 | MULTILAYERED STRUCTURE INCLUDING CYCLIC OLEFIN POLYMER OR COPOLYMER, AND PHOTOBIOREACTOR INCLUDING THE SAME - The present invention relates to multilayered structures. In various embodiments, the present invention provides an at least partially transparent multilayered structure that includes at least one first layer including at least one polyolefin, and at least one second layer including at least one of a cyclic olefin copolymer and a cyclic olefin polymer. In various embodiments, the present invention provides methods of making such multilayered structures, and photobioreactors having compartments including such multilayered structures. | 2014-06-19 |
20140170743 | PHOTOBIOREACTOR FOR CULTURING MICROALGAE - Disclosed is a structure of a photobioreactor for culturing microalgae capable of enhancing carbon dioxide (CO | 2014-06-19 |
20140170744 | CELL CULTURE DEVICE - There is provided a cell culture device including a cell chip accommodation unit accommodating a cell chip therein, a drug storage unit storing a drug and having a closed structure, a first pipe connecting the drug storage unit and the cell chip accommodation unit and transferring the drug from the drug storage unit to the cell chip accommodation unit, a second pipe connecting the drug storage unit and the cell chip accommodation unit and transferring the drug from the cell chip accommodation unit to the drug storage unit, and a pump circulating the drug between the cell chip accommodation unit and the drug storage unit. | 2014-06-19 |
20140170745 | Cell-Matrix Microspheres, Methods for Preparation and Applications - A method has been developed to produce stable cell-matrix microspheres with up to 100% encapsulation efficiency and high cell viability, using matrix or biomaterial systems with poor shape and mechanical stability for applications including cell therapeutics via microinjection or surgical implantation, 3D culture for in vitro expansion without repeated cell splitting using enzymatic digestion or mechanical dissociation and for enhanced production of therapeutic biomolecules, and in vitro modeling for morphogenesis studies. The modified droplet generation method is simple and scalable and enables the production of cell-matrix microspheres when the matrix or biomaterial system used has low concentration, with slow phase transition, with poor shape and mechanical stability. | 2014-06-19 |
20140170746 | METHOD FOR PREPARING INDUCED PLURIPOTENT STEM CELLS USING MICROVESICLES DERIVED FROM EMBRYONIC STEM CELLS - Provided is a method of dedifferentiating somatic cells using embryonic stem cell-derived microvesicles. Particularly, a method of preparing induced pluripotent stem cells by treating a composition including embryonic stem cell-derived microvesicles to the somatic cells. According to the method of preparing induced pluripotent stem cells, the dedifferentiation of the somatic cells may be efficiently performed without side effects using the embryonic stem cell-derived microvesicles, and moreover, the method is expected to be very useful in developing a cell therapy product having immunocompatibilities by individuals. | 2014-06-19 |
20140170747 | CAP FILTRATION TOOL AND TRANSFER SYSTEM - A cap has recess containing a porous frit and a graded porosity filter located so fluid from a container to which the cap is connected passes through the filter and out a spout on the cap. The filter porosity is selected to pass product expressed from cellular organisms grown in growth media inside the container while filtering out debris. A cap vent allows positive pressure in the container ullage to expedite filtering and reduce agitation of the container contents. This is not just a cap with a filter but an enabling tool. This tool enables total design change in many processes from previous thinking, allowing for the maximization of purified material, and reduction of multiple steps. | 2014-06-19 |
20140170748 | Nutrient Enriched Media for hUTC Growth - This invention provides for methods of growing anchorage-dependent cells (e.g. hUTC) in culture medium comprising amino acids, vitamins, salts nucleosides, insulin, transferrin, ethanolamine and sodium selenium, wherein the culture medium is supplemented with serum. The method further comprises addition of a serum-free nutrient solution comprising amino acids, vitamins, salts nucleosides, insulin, transferrin, ethanolamine and sodium selenium. The invention also provides for culture media and serum-free nutrient solutions for growing anchorage-dependent cells. | 2014-06-19 |
20140170749 | MULTIPOTENT STEM CELLS AND USES THEREOF - The invention provides a quiescent stem cell having the capacity to differentiate into ectoderm, mesoderm and endoderm, and which does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CD34, CD49c, CD73, CD105, CD90, CD66A, CD66E, CXCR4, CD133 or an SSEA. The invention further provides a proliferative stem cell, which expresses genes including Oct-4, Nanog, Sox2, GDF3, P16INK4, BMI, Notch, HDAC4, TERT, Rex-1, TWIST, KLF-4 and Stella but does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CF34, CF49c, CD73, CD105, CD90, CD66A, CD66E, CXCR4, CD133 or an SSEA. The cells of the invention can be isolated from adult mammals, have embryonic cell characteristics, and can form embryoid bodies. Methods for obtaining the stem cells, as well as methods of treating diseases and differentiated stem cells, are also provided. | 2014-06-19 |
20140170750 | MODULATING REGULATORY T CELL ACTIVITY VIA INTERLEUKIN 35 - Methods for regulating T cell function in a subject, particularly regulatory T cell activity are provided. Methods of the invention include administering to a subject a therapeutically effective amount of an Interleukin 35-specific binding agent, such as an antibody or small molecule inhibitor. The invention further provides methods for enhancing the immunogenicity of a vaccine or overcoming a suppressed immune response to a vaccine in a subject, including administering to the subject a therapeutically effective amount of an IL35-specific binding agent and administering to the subject a vaccine. In one embodiment the vaccine is a cancer vaccine. | 2014-06-19 |
20140170751 | METHOD FOR PREPARING CORNEAL ENDOTHELIAL CELL - The present invention relates to a method for preparing corneal endothelial progenitor cells by adherent culturing a cell population isolated from corneal endothelial cell tissue at a low density by using a serum-free medium. The present invention also relates to a method for preparing corneal endothelial cells by differentiation-inducing the corneal endothelial progenitor cells obtained by the aforementioned method. According to the present invention, corneal endothelial progenitor cells can be selectively grown from a corneal tissue-derived cell population, and corneal endothelial cells obtained by inducing the corneal endothelial progenitor cells can be applied to treatment of corneal endothelial diseases. As a result, problems of corneal transplantation such as shortage of donors and occurrence of rejection can be solved. | 2014-06-19 |
20140170752 | GENERATION OF iPS CELLS AND ASSOCIATED METHODS - Systems, constructs, and methods for reprogramming cells are provided. In one aspect, for example, a transformation construct for generating iPS cells can include an expression vector having a plurality of reprogramming factors, each reprogramming factor being under control of a separate promoter. | 2014-06-19 |
20140170753 | CRISPR-CAS SYSTEMS AND METHODS FOR ALTERING EXPRESSION OF GENE PRODUCTS - The invention provides for systems, methods, and compositions for altering expression of target gene sequences and related gene products. Provided are vectors and vector systems, some of which encode one or more components of a CRISPR complex, as well as methods for the design and use of such vectors. Also provided are methods of directing CRISPR complex formation in eukaryotic cells and methods for utilizing the CRISPR-Cas system. | 2014-06-19 |
20140170754 | METHOD FOR DIAGNOSING CORROSION OF UNDERGROUND STORAGE TANK SYSTEM - A method for diagnosing corrosion of an underground storage tank system is provided. The method includes the following steps. A sample from the underground storage tank system is collected, wherein the sample comprises at lease one metal ion. The species and the concentration of the metal ion in the sample are detected by an analysis instrument. A concentration threshold value is determined from a database according to the species of the metal ion. A mapping step is performed, wherein the concentration of the metal ion and the concentration threshold value are compared to diagnose if the underground storage tank system is corroded. | 2014-06-19 |
20140170755 | METHOD TO EVALUATE THE QUANTITY OF METHANE PRODUCED BY A DAIRY RUMINANT AND METHOD TO REDUCE AND CONTROL THIS QUANTITY - The present invention relates in particular to a method to evaluate the quantity of methane produced by a dairy ruminant. The method comprises determination of the ratio between the quantity of fatty acids with 16 carbon atoms or less and the sum of the total fatty acids of the milk, said quantity of methane being defined by the following equation: | 2014-06-19 |