| 23rd week of 2015 patent applcation highlights part 23 |
| Patent application number | Title | Published |
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| 20150152391 | Host Cells with Artificial Endosymbionts - The present invention is directed generally to eukaryotic host cells comprising artificial endosymbionts and methods of introducing artificial endosymbionts into eukaryotic host cells. The invention provides artificial endosymbionts that introduce a phenotype to host cells that is maintained in daughter cells. The invention additionally provides eukaryotic host cells containing magnetotactic bacteria. | 2015-06-04 |
| 20150152392 | HCV FULL-LENGTH INFECTIOUS CELL CULTURE SYSTEMS AND APPLICATIONS THEREOF - The present invention relates to nucleic acid sequences that encode hepatitis C viruses (HCV) that are useful in the fundamental research of HCV as well as in the search of a vaccine against HCV. In particular the present invention relates to nucleic acid sequences that comprises HCVs which are capable of expressing said virus when transfected into cells and are capable of infectivity in vivo. | 2015-06-04 |
| 20150152393 | Avian Adenoassociated Virus and Uses Thereof - The present invention provides an Avian adeno-associated virus (AAAV) virus and vectors and particles derived therefrom. In addition, the present invention provides methods of delivering a nucleic acid to a cell using the AAAV vectors and particles. Methods of isolating the AAAV are provided | 2015-06-04 |
| 20150152394 | FLAVIN-CONJUGATED GLUCOSE DEHYDROGENASE AND POLYNUCLEOTIDE ENCODING THE SAME - The purpose of the invention is to provide flavin-conjugated glucose dehydrogenase having little variation in activity in the typical biosensor measurement temperature range (10-40° C.) and a method for measuring glucose using same. The present invention relates to flavin-conjugated glucose dehydrogenase having the following properties (1)-(3) and the like: (1) action: shows glucose dehydrogenase activity in the presence of an electron acceptor; (2) substrate specificity: the activity value is 10% or less relative to maltose, D-galactose, D-fructose, sorbitol, lactose, and sucrose when the activity value relative to D-glucose is taken to be 100%; and (3) temperature characteristics: the range of the activity value at 10-40° C. is 20-150% when the activity value at 30° C. is taken to be 100%. | 2015-06-04 |
| 20150152395 | CHIMERIC LUCIFERASES - Described herein are novel chimeric luciferase molecules with enhanced properties, and methods of using these chimeric luciferase molecules. | 2015-06-04 |
| 20150152396 | Novel DNA Polymerases - Novel proteins having DNA polymerase are described which have utility in amplification reactions and have improved properties over Bst polymerase such as for example enhanced reverse transcriptase activity. | 2015-06-04 |
| 20150152397 | POLYPEPTIDES HAVING ORGANOPHOSPHOROUS HYDROLASE ACTIVITY - The present invention relates to isolated polypeptides having organophosphorous hydrolase activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2015-06-04 |
| 20150152398 | Endoribonuclease and Methods of Use Thereof - The present disclosure provides variant Cas endoribonucleases, nucleic acids encoding the variant Cas endoribonucleases, and host cells genetically modified with the nucleic acids. The variant Cas endoribonucleases find use in a variety of applications, which are also provided. The present disclosure also provides methods of detecting a specific sequence in a target polyribonucleotide; and methods of regulating production of a target RNA in a eukaryotic cell. | 2015-06-04 |
| 20150152399 | Therapeutic Nuclease Compositions and Methods - Hybrid nuclease molecules and methods for treating an immune-related disease or disorder in a mammal, and a pharmaceutical composition for treating an immune-related disease in a mammal. | 2015-06-04 |
| 20150152400 | USE OF CO2 TO DEACTIVATE A CELLULOLYTIC MICROORGANISM USED IN THE BIOCHEMICAL CONVERSION OF LIGNOCELLULOSIC MATERIALS - A process for the production of an enzymatic cocktail with a cellulolytic microorganism comprises two phases: | 2015-06-04 |
| 20150152401 | ALPHA AMYLASE VARIANTS DERIVED FROM THE ALPHA AMYLASE OF CYTOPHAGA SP. AMYLASE (CSPAMY2) - Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing. | 2015-06-04 |
| 20150152402 | METHOD OF PRODUCING RECOMBINANT PROTEINS WITH MANNOSE-TERMINATED N-GLYCANS - We describe a method of expressing a recombinant protein comprising mannose-terminated N-glycans from a host cell, the method comprising: (a) introducing a nucleic acid encoding a recombinant protein into a Chinese Hamster Ovary (CHO) cell comprising a mutation in the GnT 1 gene (GenBank Accession Number AF343963) leading to loss of GnT 1 function; and (c) expressing the recombinant protein from the host cell, in which the expressed recombinant protein comprises a mannose-terminated glycan structure, and in which the method does not include a step of introducing functional GnT-I into the host cell. The method may be used for producing recombinant glucocerebrosidase with a mannose-terminated glycan structure, suitable for treatment or prevention of Gaucher's Disease. | 2015-06-04 |
| 20150152403 | Mutant Proteinase with Reduced Self-Cleavage Activity and Method of Purification - The present invention provides a mutant 27 kDa NIa proteinase having reduced self-cleavage activity relative to the self-cleavage activity of its wild-type proteinase. The mutant has the same substrate cleavage activity as the wild-type proteinase but is more stable than the wild-type proteinase. The present invention also provides a method of obtaining large quantities of active 27 kDa NIa proteinase for use as a tool for purification of other proteins. | 2015-06-04 |
| 20150152404 | PROTEASES PRODUCING AN ALTERED IMMUNOLOGICAL RESPONSE AND METHODS OF MAKING AND USING THE SAME - The present invention provides novel protein variants that exhibit reduced immunogenic responses, as compared to the parental proteins. The present invention further provides DNA molecules that encode novel variants, host cells comprising DNA encoding novel variants, as well as methods for making proteins less allergenic. In addition, the present invention provides various compositions that comprise these proteins that are less immunogenic than the wild-type proteins. | 2015-06-04 |
| 20150152405 | SUSPENSION CONTAINER FOR BINDING PARTICLES FOR THE ISOLATION OF BIOLOGICAL MATERIAL - A device, method and system is provided for binding particles for the separation and/or isolation of biological materials. In particular, a container is provided including a suspension of binding particles for the isolation of biological material, inner walls forming an elongate groove at the bottom of said container, and a cover having openings and/or being penetrable for a linear arrangement of multiple pipets or pipet tips, said openings being located above and parallel to said elongate groove. | 2015-06-04 |
| 20150152406 | TRANSPOSITION-MEDIATED IDENTIFICATION OF SPECIFIC BINDING OR FUNCTIONAL PROTEINS - The method disclosed herein describes a novel technology offering unparalleled efficiency, flexibility, utility and speed for the discovery and optimization of polypeptides having desired binding specificity and/or functionality, including antigen-binding molecules such as antibodies and fragments thereof, for desired functional and/or binding phenotypes. The novel method is based on transposable constructs and diverse DNA libraries cloned into transposable vectors and their transfection into host cells by concomitant transient expression of a functional transposase enzyme. This ensures an efficient, stable introduction of the transposon-based expression vectors into vertebrate host cells in one step, which can then be screened for a desired functional or binding phenotype of the expressed proteins, after which the relevant coding sequences for the expressed proteins, including antibodies and fragments thereof, can be identified by standard cloning and DNA sequencing techniques. | 2015-06-04 |
| 20150152407 | Method of Normalizing Biological Samples - The present disclosure relates to normalization of biological samples, particularly samples comprising nucleic acids to be sequenced. The normalization protocols described herein may be utilized across multiple samples to cap total stoichiometric input and minimize variations in transcript abundance on a per-sample basis in a multiplexed fashion to dramatically increase the accuracy, capacity and efficiency of nucleic acid sequencing. | 2015-06-04 |
| 20150152408 | COMPOSITIONS AND METHODS FOR PROTEIN PRODUCTION - The present invention provides a method of selecting a mRNA for production of a polypeptide of interest comprising: a) producing an array of individual mRNA sequences comprising different nucleotide sequences encoding the polypeptide of interest; b) determining one or more or two or more of the following parameters for each individual mRNA sequence of (a): (i) minimum free energy (MFE) RNA secondary structure; (ii) ensemble free energy (EFE); (iii) frequency of the minimum free energy (FMFE) RNA secondary structure in a thermodynamic ensemble; and (iv) ensemble diversity (ED); c) ranking the individual mRNA sequences of the array according the parameters determined in step (b); and d) selecting a mRNA sequence from the ranked array of step (c), wherein the selected mRNA produces the polypeptide of interest. The present invention further provides a method of selecting a mRNA for enhanced and reduced production of a polypeptide of interest. | 2015-06-04 |
| 20150152409 | NUCLEIC ACID TRANSCRIPTION METHOD - The present invention relates to methods for generating an amplified nucleic acid portion of a template nucleic acid, comprising obtaining said template nucleic acid, annealing at least one oligonucleotide primer to said template nucleic acid, annealing at least one oligonucleotide stopper to said template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner until the elongating product nucleic acid reaches the position of an annealed oligonucleotide stopper, whereby the elongation reaction is stopped, wherein in said elongation reaction said oligonucleotide stopper is not elongated, and wherein the elongated product nucleic acid is ligated to the 3′ end of said oligonucleotide stopper—said stopper may also be a primer itself and uses and kits for performing said method. | 2015-06-04 |
| 20150152410 | COMPOSITIONS AND METHODS FOR MODULATING MECP2 EXPRESSION - Aspects of the invention provide single stranded oligonucleotides for activating or enhancing expression of MECP2. Further aspects provide compositions and kits comprising single stranded oligonucleotides for activating or enhancing expression of MECP2. Methods for modulating expression of MECP2 using the single stranded oligonucleotides are also provided. Further aspects of the invention provide methods for selecting a candidate oligonucleotide for activating or enhancing expression of MECP2. | 2015-06-04 |
| 20150152411 | METHODS AND COMPOSITIONS FOR MODULATING APOLIPOPROTEIN (A) EXPRESSION - Disclosed herein are antisense compounds and methods for decreasing apo(a) to treat, prevent, ameliorate diseases, disorders or conditions related to apo(a) or Lp(a). Certain diseases, disorders or conditions related to apo(a) or Lp(a) include inflammatory, cardiovascular and/or metabolic diseases, disorders or conditions. The antisense compounds disclosed herein can be used to treat such diseases, disorders or conditions in an individual in need thereof. | 2015-06-04 |
| 20150152412 | SIRNA COMPOUNDS COMPRISING TERMINAL SUBSTITUTIONS - The invention relates to modified siRNA compounds which down-regulate target gene expression, to pharmaceutical compositions comprising such compounds and to methods of treating and/or preventing the incidence or severity of various diseases or conditions associated with the genes and/or symptoms associated with such diseases or conditions. | 2015-06-04 |
| 20150152413 | Composition and Method for In Vivo and In Vitro Attenuation of Gene Expression Using Double Stranded RNA - Introduction of double stranded RNA into cells, cell culture, organs and tissues, and whole organisms, particularly vertebrates, specifically attenuates gene expression. | 2015-06-04 |
| 20150152414 | CHEMICAL MODIFICATION OF SHORT SMALL HAIRPIN RNAS FOR INHIBITION OF GENE EXPRESSION - Aspects of the present invention include the production and use of chemically modified RNAi agents (e.g., shRNAs) in gene silencing applications. The chemically modified RNAi agents disclosed herein have reduced immunostimulatory activity, increased serum stability, or both, as compared to a corresponding RNAi agent not having the chemical modification. Compositions containing chemically modified RNAi agents according to aspects of the present invention (including pharmaceutical compositions) and kits containing the same are also provided. | 2015-06-04 |
| 20150152415 | MULTIPLE EXON SKIPPING COMPOSITIONS FOR DMD - Provided are antisense molecules capable of binding to a selected target site in the human dystrophin gene to induce exon skipping, and methods of use thereof to treat muscular dystrophy. | 2015-06-04 |
| 20150152416 | NUCLEIC ACID MOLECULES AND COLLECTIONS THEREOF, THEIR APPLICATION AND MODIFICATION - The invention provides a method for characterising a sample comprising nucleic acid derived from a cell. The method comprises determining whether a sample comprises at least a minimal sequence of at least one new microRNA (miRNA) according to the invention or a mammalian ortholog thereof and characterizing the sample on the basis of the presence or absence of the miRNA. The invention further provides nucleic acid molecules and collections thereof and their use in therapeutic and diagnostic applications. The invention furthermore provides a method for identifying a miRNA molecule or a precursor molecule thereof. | 2015-06-04 |
| 20150152417 | TREATMENT OF FILAGGRIN (FLG) RELATED DISEASES BY MODULATION OF FLG EXPRESSION AND ACTIVITY - The present invention relates to antisense oligonucleotides and/or compounds that modulate the expression of and/or function of Filaggrin (FLG), in particular, by targeting natural antisense polynucleotides of Filaggrin (FLG). The invention also relates to the identification of these antisense oligonucleotides and/or compounds and their use in treating diseases and disorders associated with the expression of FLG. | 2015-06-04 |
| 20150152418 | METHODS OF TREATING VASCULAR INFLAMMATORY DISORDERS - Provided are methods of treating or delaying the onset of a vascular inflammatory disease (e.g., acute lung injury) in a subject including administering to the subject a therapeutically effective amount of a nucleic acid containing all or a part of the sequence of mature miR-181b (SEQ ID NO: 1). Also provided are methods of decreasing nuclear factor-κβ (NF-κβ) signaling in an endothelial cell including administering to the subject a nucleic acid containing all or a part of the sequence of mature miR-181b (SEQ ID NO: 1). | 2015-06-04 |
| 20150152419 | TREATMENT OF COLONY-STIMULATING FACTOR 3 (CSF3) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO CSF3 - The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Colony-stimulating factor 3 (CSF3), in particular, by targeting natural antisense polynucleotides of Colony-stimulating factor 3 (CSF3). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of CSF3. | 2015-06-04 |
| 20150152420 | COMPOUNDS AND METHODS FOR ALTERING RSV REPLICATION RATE - Provided herein are methods for altering respiratory syncytial virus (RSV) replication in a cell using oligonucleotides derived from tRNAs, also referred to as tRFs (tRNA-derived RNA Fragments). The oligonucleotides may be used to decrease or increase replication of RSV. Also provided herein are methods for treating a subject having or at risk of having an RSV infection, and animal models for evaluating viral and host factors in RSV pathogenesis. | 2015-06-04 |
| 20150152421 | HEXIM-1 AS A TARGET OF LEPTIN SIGNALING TO REGULATE OBESITY AND DIABETES - Provided are Hexim-1 inhibitor compositions, and methods and kits using said compositions for the treating and regulating obesity and/or diabetes, comprising administering an effective amount of a Hexim-1 inhibitor. Additionally provided are methods for screening for inhibitors of Hexim-1. | 2015-06-04 |
| 20150152422 | MIRNAS AS THERAPEUTIC TARGETS IN CANCER - MicroRNAs (miRNAs) are a class of non-coding small RNA molecules that regulate gene expression at the post-transcriptional level by interacting with 3′ untranslated regions (UTRs) of their target mRNAs. The invention relates to the application of miR-192 and miR-215. Both of these miRNAs impact cellular proliferation through the p53-miRNA circuit, and interact with dihydrofolate reductase (DHFR) and thymidylate synthase (TS). Particularly, upregulation of these miRNAs reduces cellular proliferation. The invention relates to this discovery. For example, inhibiting miR-192 and/or miR-215 sensitizes a neoplasm or a subject with a neoplasm to chemotherapeutic agents. Furthermore, measuring the levels of miR-192 and/or miR-215 provides one with information regarding whether the neoplasm or subject will respond to chemotherapeutic agents. Accordingly, the invention relates to composition and methods relating to the identification, characterization and modulation of the expression of miR-192 and miR-215. | 2015-06-04 |
| 20150152423 | METHOD FOR ENHANCED FERMENTATION THROUGH THE DESTRUCTION OF MITOCHONDRIAL DNA IN YEAST - Methods for enhanced yeast fermentation of plant material through the genetic modification of yeast comprising stably integrating into a yeast an inducible promoter operably linked to a mitochondrial targeting signal as well as at least one restriction enzyme and inducing the expression of the at least one restriction enzyme, wherein the restriction enzyme targets and destroys the mitochondrial DNA of the yeast. DNA constructs comprising at least an inducible promoter operably linked to a mitochondrial targeting signal as well as at least one restriction enzyme for induced expression of at least one restriction enzyme for destruction of mitochondrial DNA as well as transgenic yeast expressing a DNA construct for destruction of mitochondrial DNA and enhanced fermentation, are also disclosed. | 2015-06-04 |
| 20150152424 | TREATMENT OF PYRROLINE-5-CARBOXYLATE REDUCTASE 1 (PYCR1) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO PYCR1 - The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Pyrroline-5-carboxylate reductase 1 (PYCR1), in particular, by targeting natural antisense polynucleotides of Pyrroline-5-carboxylate reductase 1 (PYCR1). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of PYCR1. | 2015-06-04 |
| 20150152425 | TREATMENT OF METHIONINE SULFOXIDE REDUCTASE A (MSRA) RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO MSRA - The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of Methionine Sulfoxide Reductase A (MSRA), in particular, by targeting natural antisense polynucleotides of Methionine Sulfoxide Reductase A (MSRA). The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of MSRA. | 2015-06-04 |
| 20150152426 | TREATMENT OF PAR4 RELATED DISEASES BY INHIBITION OF NATURAL ANTISENSE TRANSCRIPT TO PAR4 - The present invention relates to antisense oligonucleotides that modulate the expression of and/or function of PAR4, in particular, by targeting natural antisense polynucleotides of PAR4. The invention also relates to the identification of these antisense oligonucleotides and their use in treating diseases and disorders associated with the expression of PAR4. | 2015-06-04 |
| 20150152427 | METHOD TO ENGINEER MAMMANLIAN-TYPE CARBOHYDRATE STRUCTURES - The present invention relates to host cells having modified lipid-linked oligosaccharides which may be modified further by heterologous expression of a set of glycosyltransferases, sugar transporters and mannosidases to become host-strains for the production of mammalian, e.g., human therapeutic glycoproteins. The process provides an engineered host cell which can be used to express and target any desirable gene(s) involved in glycosylation. Host cells with modified lipid-linked oligosaccharides are created or selected. N-glycans made in the engineered host cells have a GlcNAcMan | 2015-06-04 |
| 20150152428 | UNIQUE MODULAR VECTOR DESIGN - The current invention provides a modular vector system that enables the insertion of gene expression cassettes in a recursive directional stacking fashion by rare restriction sites which requires only one type of vector. The invention also provides DNA molecules, compositions, and transgenic organisms, plants, plant tissues, plant seeds, and cells comprising recombined restriction sites for rare restriction enzymes. | 2015-06-04 |
| 20150152429 | Genetic reduction of male fertility in plants - A nuclear maize gene is mutated to result in dominant male sterility. Two mutants are provided which impact signal peptide processing. Restoration constructs and methods are provided for use of the dominant male-sterile mutants in producing hybrid seed for male-sterile hybrid plants. Other signal-peptide modifications are provided. Orthologs of the wild type or mutated gene in various species are provided. | 2015-06-04 |
| 20150152430 | COMPOSITIONS AND METHODS FOR THE EXPRESSION OF A SEQUENCE IN A REPRODUCTIVE TISSUE OF A PLANT - Compositions and methods for regulating expression of heterologous nucleotide sequences in a plant are provided. Compositions include promoter sequences with direct expression in an egg cell or embryonic cell-preferred manner. Such compositions find use in, for example, a method for expressing a heterologous nucleotide sequence in a plant; detection of specific cell types in the ovule and targeted ablation of specific cell types. | 2015-06-04 |
| 20150152431 | ISOLATED POLYNUCLEOTIDES AND POLYPEPTIDES, AND METHODS OF USING SAME FOR INCREASING NITROGEN USE EFFICIENCY, YIELD, GROWTH RATE, VIGOR, BIOMASS, OIL CONTENT, AND/OR ABIOTIC STRESS TOLERANCE - Provided are methods of increasing nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant by expressing within the plant an exogenous polynucleotide comprising a nucleic acid sequence at least 80% identical to SEQ ID NO: 387, 1-386, 388-469, 763-3704 and 3705; or an exogenous polynucleotide encoding a polypeptide at least 80% identical to SEQ ID NO: 602, 470-601, 603-762, 3706-5858, 5860-5910, 5912, 5914-5923, 5925-6046 or 6047. Also provided is an isolated polynucleotide comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs: 387, 1-386, 388-469, 763-3704 and 3705, which can be used to increase the nitrogen use efficiency, yield, biomass, growth rate, vigor, oil content, fiber yield, fiber quality and/or abiotic stress tolerance of a plant. | 2015-06-04 |
| 20150152432 | PLANTS WITH INCREASED YIELD AND A METHOD FOR MAKING THE SAME - The present invention provides a method for producing a plant with increased yield as compared to a corresponding wild type plant comprising increasing or generating one or more protein activities in a plant or a part thereof. The present invention further relates to nucleic acids enhancing or improving one or more traits of a transgenic plant, and cells, progenies, seeds and pollen derived from such plants or parts, as well as methods of making and methods of using such plant cell(s) or plant(s), progenies, seed(s) or pollen. Particularly, the improved trait(s) are manifested as increased yield, preferably by improving one or more yield-related trait(s). | 2015-06-04 |
| 20150152433 | TOMATO HYBRID PX 02461513 AND PARENTS THEREOF - The invention provides seed and plants of tomato hybrid PX 02461513 and the parent lines thereof. The invention thus relates to the plants, seeds and tissue cultures of tomato hybrid PX 02461513 and the parent lines thereof, and to methods for producing a tomato plant produced by crossing such plants with themselves or with another tomato plant, such as a plant of another genotype. The invention further relates to seeds and plants produced by such crossing. The invention further relates to parts of such plants, including the fruit and gametes of such plants. | 2015-06-04 |
| 20150152434 | SIMIAN ADENOVIRUSES SAdV-43, -45, -46, -47, -48, -49, AND -50, AND USES THEREOF - A recombinant vector comprises simian adenovirus 43, 45, 46, 47, 48, 49 or 50 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus 43, 45, 46, 47, 48, 49 or 50 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided. | 2015-06-04 |
| 20150152435 | METHOD FOR EXPRESSION OF SMALL RNA MOLECULES WITHIN A CELL - The invention provides methods and compositions for the expression of small RNA molecules within a cell using a lentiviral vector. The methods can be used to express doubles stranded RNA complexes. Small interfering RNA (siRNA) can be expressed using the methods of the invention within a cell, which are capable of down regulating the expression of a target gene through RNA interference. A variety of cells can be treated according to the methods of the invention including embryos, embryogenic stem cells, allowing for the generation of transgenic animals or animals constituted partly by the transduced cells that have a specific gene or a group of genes down regulated. | 2015-06-04 |
| 20150152436 | THERAPEUTIC USES OF GENOME EDITING WITH CRISPR/Cas SYSTEMS - Disclosed herein are methods, compositions, and kits for high efficiency, site-specific genomic editing of cells for treating or preventing genetic blood disorders. | 2015-06-04 |
| 20150152437 | Chromosomal Landing Pads and Related Uses - Provided herein are methods for stable integration and/or expression of one or more recombinant polynucleotides in a host cell. The recombinant polynucleotides are typically integrated into the host genome at some native chromosomal integration sites. The integration can be mediated by homologous recombination or by using a hybrid recombinase targeting the specific chromosomal locations. The native chromosomal integration sites in the host cells, which support stable integration and strong transcription activities of foreign genes, are present within or adjacent to specific genes in the CHO genome, ankyrin 2 gene (Ank2), cleavage and polyadenylation specific factor 4 gene (Cpsf4), C-Mos gene, and Nephrocystin-1/Mal gene. Also provided are methods and nucleic acid molecules for inserting site-specific recombination sequences (chromosomal landing pads) into these specific chromosomal locations. Further provided are engineered host cells which contain chromosomal landing pads or transgenes integrated into the native chromosomal integration sites. Further provided are methods and compositions (e.g., kits) for integrating and expressing heterologous polynucleotides in host cells bearing inserted chromosomal landing pads. | 2015-06-04 |
| 20150152438 | Recombinant Synthesis of Alkanes - The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently produce alkanes, and in particular the use of such organisms for the commercial production of alkanes and related molecules. Other materials, methods, and compositions are also described. | 2015-06-04 |
| 20150152439 | MODIFIED MICROORGANISMS AND METHODS OF MAKING BUTADIENE USING SAME - The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom. | 2015-06-04 |
| 20150152440 | MODIFIED MICROORGANISMS AND METHODS OF CO-PRODUCING BUTADIENE WITH 1-PROPANOL AND/OR 1,2-PROPANEDIOL - The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in a pathway that catalyzes a conversion of a fermentable carbon source to butadiene and/or one or more polynucleotides coding for enzymes in a pathway that catalyzes a conversion of a fermentable carbon source to 1-propanol and/or 1,2-propanadiol. Also provided are methods of using the microorganisms to produce butadiene and co-products such 1-propanol and/or 1,2-propanediol. | 2015-06-04 |
| 20150152441 | METHODS AND SYSTEMS FOR IMPROVING FERMENTATION EFFICIENCY - Processes, as well as associated systems, are disclosed for biological conversion of CO into desired end products such as ethanol and 2,3-butanediol. The pre-treatment of industrial gas streams comprising CO, such that the level of contaminants selected from the group comprising benzene, toluene, xylene and ethylbenzene, are maintained below a predetermined liquid level in the fermentation broth, has been shown to have positive effects on the fermentation process. | 2015-06-04 |
| 20150152442 | METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS CLAVATUS FOR SACCHARIFICATION - A fungal α-amylase is provided from | 2015-06-04 |
| 20150152443 | Methods of Increasing Dihydroxy Acid Dehydratase Activity to Improve Production of Fuels, Chemicals, and Amino Acids - The present invention is directed to recombinant microorganisms comprising one or more dihydroxyacid dehydratase (DHAD)-requiring biosynthetic pathways and methods of using said recombinant microorganisms to produce beneficial metabolites derived from said DHAD-requiring biosynthetic pathways. In various aspects of the invention, the recombinant microorganisms may be engineered to overexpress one or more polynucleotides encoding one or more Aft proteins or homologs thereof. In some embodiments, the recombinant microorganisms may comprise a cytosolically localized DHAD enzyme. In additional embodiments, the recombinant microorganisms may comprise a mitochondrially localized DHAD enzyme. In various embodiments described herein, the recombinant microorganisms may be microorganisms of the | 2015-06-04 |
| 20150152444 | METABOLICALLY ENGINEERED CELLS FOR THE PRODUCTION OF RESVERATROL OR AN OLIGOMERIC OR GLYCOSIDICALLY-BOUND DERIVATIVE THEREOF - A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including | 2015-06-04 |
| 20150152445 | MICROORGANISMS AND METHODS FOR THE PRODUCTION OF KETONES - The invention provides a recombinant, acetogenic, carboxydotrophic bacterium that lacks secondary alcohol dehydrogenase or comprises an inactivated secondary alcohol dehydrogenase. The inactivated secondary alcohol dehydrogenase may be encoded by a secondary alcohol dehydrogenase gene comprising an inactivating mutation that reduces the ability of the bacterium to convert acetone to isopropanol and to convert methyl ethyl ketone to 2-butanol. Since a bacterium that lacks secondary alcohol dehydrogenase or comprises an inactivated secondary alcohol dehydrogenase accumulates carbonyl-containing compounds, the invention also provides a method of producing carbonyl-containing compounds, such as acetone and methyl ethyl ketone. | 2015-06-04 |
| 20150152446 | MICROBIAL ENGINEERING FOR THE PRODUCTION OF CHEMICAL AND PHARMACEUTICAL PRODUCTS FROM THE ISOPRENOID PATHWAY - The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids. | 2015-06-04 |
| 20150152447 | YEAST CELL WITH INACTIVATED GLYCEROL-3-PHOSPHATE DEHYDROGENASE AND ACTIVATED GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE AND METHOD OF PRODUCING LACTATE USING THE SAME - A genetically modified yeast cell comprising increased glyceraldehyde-3-phosphate dehydrogenase activity converting glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate as compared to a parent yeast cell of the same type, and reduced glycerol-3-phosphate dehydrogenase activity converting dihydroxyacetone phosphate to glycerol-3-phosphate compared to a parent yeast cell of the same type, and related compositions and methods. | 2015-06-04 |
| 20150152448 | D-Glucaric Acid Producing Bacterium, and Method for Manufacturing D-Glucaric Acid - The present invention provides a D-glucaric acid-producing bacterium and a method for producing D-glucaric acid. The present invention is characterized in that D-glucaric acid or a salt thereof is produced from one or more saccharides selected from the group consisting of D-glucose, D-gluconic acid and D-glucuronic acid with catalytic action of a specific alcohol dehydrogenase PQQ-ADH (1) and a specific aldehyde dehydrogenase PQQ-ALDH (2), and that D-glucaric acid or a salt thereof is produced by using a microorganism having the PQQ-ADH (1) and the PQQ-ALDH (2) or a processed product thereof in the presence of the one or more saccharides. The present invention can provide a microorganism having improved productivity of D-glucaric acid to be used for production of D-glucaric acid and a method for efficiently producing D-glucaric acid. | 2015-06-04 |
| 20150152449 | Lactate Production Process - A process for producing alkyl R-lactate from an initial compound comprising substructure (I) is provided. The process involves producing an intermediate comprising R,R- and S,S-lactide from the initial compound, wherein the initial compound is subjected to stereoisomerisation conditions; and reacting at least a portion of the intermediate with an alkyl alcohol to produce a product comprising alkyl R-lactate, wherein alkyl R-lactate is produced in the presence of an enzyme. Also provided are processes for the production of R-lactic acid, oligomeric R-lactic acid, R,R-lactide, poly-R-lactic acid and stereocomplex lactic acid. | 2015-06-04 |
| 20150152450 | POLYUNSATURATED FATTY ACID SYNTHASE NUCLEIC ACID MOLECULES AND POLYPEPTIDES, COMPOSITIONS, AND METHODS OF MAKING AND USES THEREOF - The present invention is directed to isolated nucleic acid molecules and polypeptides of thraustochytrid polyunsaturated fatty acid (PUFA) synthases involved in the production of PUFAs, including PUFAs enriched in docosahexaenoic acid (DHA), eicosapentaenoic acid (EPA), or a combination thereof. The present invention is directed to vectors and host cells comprising the nucleic acid molecules, polypeptides encoded by the nucleic acid molecules, compositions comprising the nucleic acid molecules or polypeptides, and methods of making and uses thereof. | 2015-06-04 |
| 20150152451 | PROCESS FOR THE ENANTIOSELECTIVE ENZYMATIC REDUCTION OF HYDROXY KETO COMPOUNDS - In a process for the enantioselective enzymatic reduction of a hydroxy ketone of general formula I | 2015-06-04 |
| 20150152452 | Enzymatic Oxidation of 5-Hydroxymethylfurfural and Derivatives Thereof - Provided herein are enzymatic methods for oxidation of 5-hydroxymethylfurfural (HMF) and HMF derivatives. | 2015-06-04 |
| 20150152453 | GENETICALLY MODIFIED MICROORGANISMS CAPABLE OF PRODUCING BETA-GLUCANS AND METHODS FOR PRODUCING BETA-GLUCANS - The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans. | 2015-06-04 |
| 20150152454 | METHOD FOR PRODUCING BETA-GLUCAN - It is an object of the present invention to provide a method for producing β-glucan having excellent immunopotentiating effects at a lower cost. Black yeast ( | 2015-06-04 |
| 20150152455 | Polypeptides Having Berberine Bridge Enzyme Like Activity and Polynucleotides Encoding Same - Provided are isolated polypeptides having Berberine Bridge Enzyme Like activity, catalytic domains, FAD binding domains and polynucleotides encoding the polypeptides, catalytic domains or FAD binding domains. The nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or FAD binding domains are also provided. | 2015-06-04 |
| 20150152456 | Polypeptides Having Glucuronyl Esterase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having glucuronyl esterase activity, catalytic domains and polynucleotides encoding the polypeptides or catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides or catalytic domains. | 2015-06-04 |
| 20150152457 | DIRECT STARCH TO FERMENTABLE SUGAR - Provided herein are compositions and methods related to the direct conversion of the starch in a ground or fractionated grain into a fermentable sugar feedstock capable of serving as a carbon source for the industrial production of one or more products by a fermenting organism. Such conversions may be performed at temperatures at or below the initial gelatinization temperature of the starch present in the grain and may utilize one or more isolatable endogenous enzymes present in certain unrefined grains. | 2015-06-04 |
| 20150152458 | LOW TEMPERATURE METHOD FOR MAKING HIGH GLUCOSE SYRUP - The present teachings provide a method for making a high glucose syrup at a low temperature. In some embodiments, the syrup contains reduced reversion reaction products. The method comprises contacting a starch substrate at a temperature below the starch gelatinization temperature with an enzyme blend comprising a high dose of alpha-amylase and a low dose of glucoamylase. In some embodiments, a blend of two glucoamylases is employed. In some embodiments, a debranching enzyme such as a pullulanase is employed. In some embodiments, the enzymes are staged by adding at different times, or at different temperatures. The present teachings provide for high glucose syrups with fewer reversion products, and allow for higher starch solubilization. | 2015-06-04 |
| 20150152459 | Production of Highly Purified Sodium Hyaluronate (HANA) with Controlled Molecular Weight - Disclosed is a process for the production of sodium hyaluronate with a molecular weight of between 60 and 2400 kDa and low polydispersity (1.4 Mw/Mn), which comprises: a) a step of fermentation of | 2015-06-04 |
| 20150152460 | METHODS AND COMPOSITIONS FOR SEAMLESS CLONING OF NUCLEIC ACID MOLECULES - The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention. | 2015-06-04 |
| 20150152461 | Genetically Encoded Initiator For Polymer Growth From Proteins - This invention pertains to methods for producing homogeneous recombinant proteins that contain polymer initiators at defined sites. The unnatural amino acid, 4-(2′-bromoisobutyramido)phenylalanine of formula 1, was designed and synthesized as a molecule comprising a functional group further comprising an initiator for an atom-transfer radical polymerization (‘ATRP”) that additionally would provide a stable linkage between the protein and growing polymer. We evolved a | 2015-06-04 |
| 20150152462 | Carbohydrate Esters as Inducers for Gene Expression - The invention provides novel carbohydrate esters, in particular disaccharide esters, and the methods of their preparation. These compounds find use as microbial media components for the induction of gene expression in microbial fermentation processes. | 2015-06-04 |
| 20150152463 | CELLS DEFICIENT IN CMP-N-ACETYLNEURAMINIC ACID HYDROXYLASE AND/OR GLYCOPROTEIN ALPHA-1,3-GALACTOSYLTRANSFERASE - The present invention provides non-human mammalian cell lines that are deficient in CMP-Neu5Ac hydroxylase (Cmah) and/or glycoprotein alpha-1,3-galactosyltransferase (Ggta1). Also provided are methods for using the cells disclosed herein for producing recombinant proteins with human-like patterns of glycosylation. | 2015-06-04 |
| 20150152464 | PROCESS TO CONTROL CO CONCENTRATIONS IN FERMENTATIONS - A process for controlling concentration of CO in a bioreactor provides a direct and real time measurement of dissolved CO in a fermentation medium. The process for controlling concentrations of CO in a bioreactor includes contacting an aliquot of fermentation medium with at least one CO binding ligand and at least one microbial inactivator. | 2015-06-04 |
| 20150152465 | DEVICE FOR STANDARDISING THE IN-VITRO SYNERGY TESTING OF TWO ANTIBIOTICS THROUGH THE METHOD CROSSING THE GRADIENT STRIPS - A device for microbiological analyses is provided. More specifically, a device is provided for standardising the crossing and allowing a perfect angle of 90° between two graduated paper strips impregnated with a predefined concentration gradient of an antimicrobial agent, for evaluating their synergistic effect on the minimum inhibitory concentration (MIC) on a bacterial culture medium. | 2015-06-04 |
| 20150152466 | CATALASE IN GROWTH MEDIA - The present invention relates to the use of a secreted fungal catalase, for hydrogen-peroxide neutralization in growth media for the detection of microorganisms as well as to a method for detecting microorganisms in hydrogen peroxide-bearing aerosol or on a hydrogen peroxide bearing surface, said method comprising contacting said aerosol or surface with a growth medium comprising a secreted fungal catalase, and detecting growth of microorganisms in said medium. | 2015-06-04 |
| 20150152467 | Filter Arrangement with Slider Valve and Method for Using the Same - A filter arrangement with a top element and a bottom element and a filter element therebetween captures oversized particles on the upper surface of the filter element and tangentially rinses these particles using an elution fluid to provide a concentration of particles in a relatively low volume of fluid for further analysis. A configuration using a slider valve may also be utilized. Additionally, an arrangement of supply and receiving containers may be used to minimize the number of containers required. A mass flow meter may be incorporated to measure the flow of elution fluid. Finally, a wash stage of the filtering process may be used to introduce stain onto the particles for further analysis, such as that associated with Gram staining and these stained particles may be further analyzed. | 2015-06-04 |
| 20150152468 | DISPOSABLE TEST STRIP DEVICE FOR ANALYTE DETECTION IN A BODY LIQUID SAMPLE - A disposable, enzymatic assay based test strip device for colorimetric detection of an analyte compound, such as ethanol, in a body liquid sample, such as saliva, is provided. The test strip device includes at least one reactive test zone configured to develop a visual indication when the concentration of the analyte compound in the body liquid sample exceeds a predetermined limit, and a reactive control zone configured to develop a visual indication upon being supplied with the body liquid sample. The test strip device further includes a compound buffer, preferably selected from non-volatile alcohols and disposed to closely adjoin the reactive control zone such, that the content of the compound buffer admixes with the reagents provided within the reactive control zone, while the control zone is being supplied with the body liquid sample. Deposition of the reactive zones is preferably implemented by conventional printing methods utilizing biological inks. | 2015-06-04 |
| 20150152469 | FLUORESCENT PROBE FOR HIGH-SENSITIVITY PANCREATIC FLUID DETECTION, AND METHOD FOR DETECTING PANCREATIC FLUID | 2015-06-04 |
| 20150152470 | THE USE OF ENDOTOXIN NEUTRALIZATION AS A BIOMARKER FOR INTESTINAL WALL DAMAGE - Provided herein are methods for detecting endotoxin neutralization in a subject. Also provided are methods for determining the effectiveness of a therapeutic agent for treating intestinal wall damage. Further provided are methods of treating intestinal wall damage are also provided. | 2015-06-04 |
| 20150152471 | PROTEASE DETECTION - A polypeptide comprising a chromogenic amino acid. The chromogenic amino acid is flanked by at least one amino acid to the N and C termini thereof. The amine group of the chromogenic amino acid has a pKa of less than 5. The chromogenic amino acid is capable of reacting with a conjugated aldehyde. The polypeptide comprises a target sequence for a target protease which is capable of cleaving the peptide bond comprising the amino group of the chromogenic amino acid. | 2015-06-04 |
| 20150152472 | REACTION DETECTION METHOD - A chemical reaction rate with respect to a target substance in a sample is increased. | 2015-06-04 |
| 20150152473 | IMMUNO-AMPLIFICATION - A high-sensitivity, low-background immuno-amplification assay is provided, which offers a streamlined workflow suitable for high-throughput assays of clinically relevant samples, such as blood and other bodily fluids. The assay comprises the use of two proximity members that each comprise an analyte-specific binding component conjugated to an oligonucleotide. Binding an analyte brings the oligonucleotide moieties of the proximity members in sufficiently close contact that the oligonucleotides form an amplicon. The presence of the analyte then is detected through amplification of the amplicon and detection of the amplified nucleic acids. The sensitivity of the assay of the present invention is improved by preventing spurious or non-specific amplicon formation by proximity members that are not complexed with an analyte. | 2015-06-04 |
| 20150152474 | BIOMARKER COMPOSITIONS AND METHODS - Biomarkers can be assessed for diagnostic, therapy-related or prognostic methods to identify phenotypes, such as a condition or disease, or the stage or progression of a disease, select candidate treatment regimens for diseases, conditions, disease stages, and stages of a condition, and to determine treatment efficacy. Circulating biomarkers from a bodily fluid can be used in profiling of physiological states or determining phenotypes. These include nucleic acids, protein, and circulating structures such as vesicles, and nucleic acid-protein complexes. | 2015-06-04 |
| 20150152475 | SYSTEMS AND METHODS FOR FLUORESCENCE DETECTION WITH A MOVABLE DETECTION MODULE - A fluorescence detection apparatus for analyzing samples located in a plurality of wells in a thermal cycler and methods of use are provided. In one embodiment, the apparatus includes a support structure attachable to the thermal cycler and a detection module movably mountable on the support structure. The detection module includes one or more channels, each having an excitation light generator and an emission light detector both disposed within the detection module. When the support structure is attached to the thermal cycler and the detection module is mounted on the support structure, the detection module is movable so as to be positioned in optical communication with different ones of the plurality of wells. The detection module is removable from the support structure to allow easy replacement. | 2015-06-04 |
| 20150152476 | REAGENTS AND METHODS FOR DIRECT LABELING OF NUCLEOTIDES - The present invention provides systems and methods for production of activatable diazo-derivatives for use in labeling nucleotides. Labeling nucleotides is accomplished by contacting a stable hydrazide derivative of a detectable moiety with an activating polymer reagent which is used to directly label the nucleotide sample. Labeling occurs on the phosphate backbone of the nucleotide which does not perturb hybridization of the labeled nucleotide with its anti-sense strand. Since the method involves direct labeling, all types of nucleotides can be labeled without prior amplification or alteration. | 2015-06-04 |
| 20150152477 | SYSTEMS AND METHODS FOR THERMAL ACTUATION OF MICROFLUIDIC DEVICES - A microfluidic processing device includes a substrate defining a microfluidic network. The substrate is in thermal communication with a plurality of N independently controllable components and a plurality of input output contacts for connecting the substrate to an external controller. Each component has at least two terminals. Each terminal is in electrical communication with at least one contact. The number of contacts required to independently control the N components is substantially less than the total number of terminals. Upon actuation, the components typically heat a portion of the microfluidic network and/or sense a temperature thereof. | 2015-06-04 |
| 20150152478 | GENE EXPRESSION PROFILING FROM FFPE SAMPLES - Methods and compositions relating to the generation and use of gene expression data from tissue samples that have been fixed and embedded are provided. The data can electronically stored and implemented as well as used to augment diagnosis and treatment of diseases. | 2015-06-04 |
| 20150152479 | CARTRIDGE FOR NUCLEIC ACID AMPLIFICATION REACTION AND CARTRIDGE KIT FOR NUCLEIC ACID AMPLIFICATION REACTION - A cartridge for nucleic acid amplification reaction includes a tube that is internally provided with, in the following order, in a longitudinal direction, a first plug composed of oil, a second plug composed of a first washing liquid, a third plug composed of oil, a fourth plug composed of an eluent, and a fifth plug composed of oil, and a container for nucleic acid amplification reaction that is disposed to communicate with an end portion of the tube on a side on which the fifth plug is disposed and contains oil, in which a freeze-dried nucleic acid amplification reaction reagent does not dissolve in the oil of the fifth plug and is held in the oil. | 2015-06-04 |
| 20150152480 | CONTAINER FOR NUCLEIC ACID AMPLIFICATION REACTION, CARTRIDGE FOR NUCLEIC ACID AMPLIFICATION REACTION, AND CARTRIDGE KIT FOR NUCLEIC ACID AMPLIFICATION REACTION - A container for nucleic acid amplification reaction includes a freeze-dried nucleic acid amplification reaction reagent, an oil, and a container that contains the freeze-dried nucleic acid amplification reaction reagent and the oil. The freeze-dried nucleic acid amplification reaction reagent is held in the oil. A cartridge for nucleic acid amplification reaction and a cartridge kit for nucleic acid amplification reaction are configured using the same. | 2015-06-04 |
| 20150152481 | COMPOSITIONS AND METHODS FOR DEHYDRATED STORAGE OF ON-BOARD REAGENTS IN MICROFLUIDIC DEVICES - Manufacturing methods and compositions are described for production of self-contained microfluidic cartridge devices with on-board reagents for molecular biological testing. Sensitive reagents are stored in dry form without lyophilization or freezing, and reconstituted at the point of use with either a biological sample or a sample eluate at the point of use. Manufacturing methods include sheet and roll fabrication processes where the reagents are printed in place and sealed within individual microfluidic cartridges before gel vitrification. | 2015-06-04 |
| 20150152482 | Multiplex Targeted Amplification Using Flap Nuclease - Methods for multiplex amplification of a plurality of targets of distinct sequence from a complex mixture are disclosed. In one aspect targets are circularized using a single circularization probe that is complementary to two regions in the target that flank a region to be amplified. The targets may hybridize to the circularization probe so that 5′ or 3′ flaps are generated and methods for removing flaps and circularizing the resulting product are disclosed. In another aspect targets are hybridized to dU probes so that 5′ and 3′ flaps are generated. The flaps are cleaved using 5′ or 3′ flap endonucleases or 3′ to 5′ exonucleases. The target sequences are then ligated to common primers, the dU probes digested and the ligated targets amplified. | 2015-06-04 |
| 20150152483 | FAST AND RELIABLE WINE LACTIC BACTERIA IDENTIFICATION METHOD - The invention depicts a method to identify the | 2015-06-04 |
| 20150152484 | MICROBIOME MARKERS AND THERAPIES FOR AUTISM SPECTRUM DISORDERS - The present disclosure provides for characterization of normal flora and identifying biomarkers in the gut of healthy, neruotypical subjects. Aspect of the disclosure provide for the characterization of the gut microbiome in ADS subjects, characterized by reduced richness and significant loss of the ‘ | 2015-06-04 |
| 20150152485 | MYCOBACTERIUM TUBERCULOSIS DETECTION USING TRANSRENAL DNA - The present invention relates to the field of | 2015-06-04 |
| 20150152486 | METHOD OF IN VITRO DIAGNOSING A PELLICULAR STATE IN A SUBJECT AND RELATED APPLICATIONS - The present invention relates to a method of diagnosing a pellicular state in a subject, to a screening method of identifying an antidandruff agent, and to kits and nucleic acid arrays useful for said methods. | 2015-06-04 |
| 20150152487 | Lactobacillus Mutant, Nucleotide Sequence for Lactobacillus Mutant and Primers for Nucleotide Sequence of Lactobacillus Mutant - The present invention relates to a lactobacillus mutant, a nucleotide sequence for | 2015-06-04 |
| 20150152488 | Method for Identification of Sensitivity of a Patient to Telomerase Inhibition Therapy - The invention provides methods for determining the susceptibility of cancer patients to developing adverse reactions if treated with a telomerase inhibitor drug by measurement of telomere length in appropriate cells of the patient prior to initiation of the telomerase inhibitor treatment. | 2015-06-04 |
| 20150152489 | MICROBEAD AGGLUTINATION BASED ASSAYS - A method for detecting the presence of an analyte in a sample can include adding a plurality of microparticles of a first-type to the sample, where each microparticle of the first-type includes a first binding partner configured to interact with at least a first portion of the analyte, adding a plurality of microparticles of a second-type to the sample, where each microparticle of the second-type includes a second binding partner configured to interact with at least a second portion of the analyte, the first portion of the analyte being different from the second portion of the analyte, and identifying an aggregate including at least one microparticle of the first-type, at least one microparticle of the second-type and the analyte, where the aggregate indicates the presence of the analyte. | 2015-06-04 |
| 20150152490 | FLUORESCENT NANOPARTICLE COMPOSITES THEMSELVES, PROCESS FOR THE PREPARATION OF SUCH COMPOSITES, AND USE IN RAPID DIAGNOSIS SYSTEMS WITH AFFINITY TO BIOLOGICAL MOLECULES - The present invention provides fluorescent nanoparticle composites themselves, the process of preparing such composites, to systems for rapid diagnosis (as “kits”) containing such composites, and to the use of such composites. In a preferential embodiment, the composites of the present invention have an affinity for biological molecules, such as DNA. The present invention also comprises the preparation of probes containing biological material, upon which are added fluorescent nanoparticle composites, making viable a rapid and economic biological diagnosis of, for example, diseases and genetic traits, notably in the medical and veterinarian fields. There is yet the fact that the absorption of radiation in the ultraviolet and visible regions provides advantageous use of its fluorescent properties in photovoltaic or electroluminescent devices, such as organic LEDs, or for the increase in luminous gain of fluorescent lamps, which represents another characteristic of the invention. | 2015-06-04 |
