22nd week of 2012 patent applcation highlights part 38 |
Patent application number | Title | Published |
20120135420 | Method for detecting the presence of target bacteria or a target component carbohydrate antigen thereof - A process is disclosed for separating a carbohydrate antigen from a Gram-positive or Gram-negative bacteria in a purified form that contains no more than 10% protein. The separated antigen is coupled to an affinity column, over which polyclonal antibodies to the same bacteria are chromatographed and recovered in a purified form that exhibits high specificity and sensitivity in immunoassays for the raw carbohydrate antigen corresponding to the purified antigen on the column. A particularly preferred form of rapid immunochromatographic assay employing the purified antibodies, which assay is very useful as an aid to rapid diagnosis of diseases caused by bacteria, is disclosed. | 2012-05-31 |
20120135421 | METHODS FOR THE IDENTIFICATION OF PHOSPHATIDYLINOSITOL KINASE INTERACTING MOLECULES AND FOR THE PURIFICATION OF PHOSPHATIDYLINOSITOL KINASE PROTEINS - The present invention relates to immobilization compounds of formula (I), immobilization products and preparations thereof as well as methods and uses for the identification of phosphatidylinositol kinase interacting compounds or for the purification or identification of phosphatidylinositol kinase proteins. | 2012-05-31 |
20120135422 | ANTI-GPCR ANTIBODY AND THE METHOD OF PRODUCING THE SAME - Disclosed is a discussion of a method for producing anti-mammalian GPCR antibody and of the antibody itself. Anti-mammalian GPCR antibody is produced through immunization involving exposure of fish to full-length or truncated mammalian GPCR, and anti-mammalian GPCR antibody that can be obtained by this method is also discussed. | 2012-05-31 |
20120135423 | Methods for GPCR signaling pathway determination using biosensor-cell assays - A system and method for GPCR signaling pathway analysis and elucidation using a biosensor, a live-cell, and a pathway active compound, as defined herein. | 2012-05-31 |
20120135424 | MER DIAGNOSTIC AND THERAPEUTIC AGENTS - Mer diagnostic and therapeutic agents are disclosed. The agents are useful in the diagnosis and treatment of a variety of diseases including leukemia, lymphoma, and clotting disorders. | 2012-05-31 |
20120135425 | ELISA for Haptoglobin-Matrix Metalloproteinase 9 Complex as a Diagnostic Test for Conditions Including Acute Inflammation - A method for detecting a haptoglobin-matrix metalloproteinase 9 (Hp-MMP 9) complex in a biological sample. The sample includes incubating the biological sample with a capture reagent immobilized on a solid support to bind Hp-MMP 9 to the capture reagent. The capture reagent includes a monoclonal antibody that binds MMP9. The method detects Hp-MMP 9 bound to the immobilized capture reagent by contacting the bound Hp-MMP 9 with a detectable antibody that binds to Hp. | 2012-05-31 |
20120135426 | Method for quantifying phosphokinase activity on proteins - The invention involves a method for measuring phosphorylation of proteins at specific sites and, as such, is an indicator of the protein kinase activity of enzymes capable of phosphorylating those sites. The method involves the in vitro or in vivo phosphorylation of a target protein at a specific serine, threonine or tyrosine residue, subjecting that protein (non-phosphorylated) to reaction mixture containing all reagents, including phosphokinase which allow the creation of a phosphorylated form of protein. The phosphorylated protein is measured by contacting it with an antibody specific for the phosphorylation site(s). The invention includes antibodies useful in practicing the methods of the invention. The invention particularly relates to all proteins modified by phosphorylation and dephosphorylation as illustrated by Tau, Rb and EGFR proteins and antibodies specific for the site of phosphorylation of the Tau, Rb or EGFR proteins. | 2012-05-31 |
20120135427 | METHODS FOR DETERMINING MATERNAL HEALTH RISKS - The present description relates to a method for determining the risk of a pregnant woman developing a hypertensive disorder, more specifically gestational hyper-tension or late onset preeclampsia. The present description provides methods useful for determining risk that a pregnant individual will develop a hypertensive disorder or condition of pregnancy, such as gestational hypertension, early preeclampsia, late preeclampsia and related disorders. Several useful combinations of biochemical markers and related clinical population studies are described herein. Additionally, it is proposed herein that certain sets of biochemical markers can be used to determine risk of multiple hypertensive disorders in a single screen. The biochemical markers are PlGF, Activin A and optionally P-Selectin. | 2012-05-31 |
20120135428 | NOVEL MARKERS FOR CHRONIC KIDNEY DISEASE - The present invention relates to a method for determining the progression of chronic kidney disease in a subject suspected of having chronic kidney disease, comprising of determining the expression levels of at least one marker selected from (a) FGF23; and (b) adiponectin in a biological sample. Furthermore, the present invention relates to a use of a specific detection molecule for FGF23 or adiponectin for the preparation of a diagnostic composition for the detection of chronic kidney disease or the progression of chronic kidney diseases in a subject suspected to suffer from said disease. In particular, the present invention also provides for use of FGF23 and/or of adiponectin as an in vitro marker for the presence, absence or progression of a chronic kidney disease and kits comprising a specific detection molecule for FGF23 or a specific detection molecule for adiponectin for use in the method of the present invention. | 2012-05-31 |
20120135429 | PRO-GRP AS A SURROGATE MARKER TO PREDICT AND MONITOR RESPONSE TO BCL-2 INHIBITOR THERAPY - A method for classifying cancer patients as eligible to receive cancer therapy with a Bcl-2 inhibitor comprising determination of the presence or absence in a patient tissue sample of levels of pro-GRP, as a surrogate marker for the presence of chromosomal copy number gain at chromosomal locus 18q21-q22. The classification of cancer patients based upon pro-GRP levels as a surrogate for the presence or absence of 18q21-q22 gain allows selection of patients to receive chemotherapy with a Bcl-2 family inhibitor, either as monotherapy or as part of combination therapy, and to monitor patient response to such therapy using a peripheral blood sample. | 2012-05-31 |
20120135430 | COMPLEMENT ASSAYS AND USES THEREOF - The present invention provides methods for assessing complement activation and methods for assessing the ability of an agent or condition of interest to modulate complement activation. The present invention provides methods for assessing whether a subject has or is at increased risk of developing a complement-mediated disorder, e.g., age-related macular degeneration (AMD). Also provided are kits containing materials useful for performing the methods. | 2012-05-31 |
20120135431 | TYPE IV COLLAGEN-LIKE IMMUNOREACTIVE PEPTIDE - A type IV collagen-like immunoreactive peptide and an antibody thereof which are useful for detecting nephritis, a method for selecting a type IV collagen-like immunoreactive peptide, a method for screening an immunoreactive antibody and an immunoreactive peptide, a nephritis model, a method for detecting chronic nephritis, a vaccine, and a therapeutic agent for nephritis are provided. | 2012-05-31 |
20120135432 | DIAGNOSTIC METHOD FOR DETERMINING THE SUSCEPTIBILITY TO DELIVERY AND REAGENT KIT FOR USE THEREOF - The invention relates to a diagnostic method for detecting susceptibility to delivery, and to a test kit for this purpose. A low, but higher than baseline level concentration of Insulin-like Growth Factor Binding Protein 1 (IGFBP-1), which is due to leakage from decidual cells, is detected by an immunological assay in a vaginal secretion sample. | 2012-05-31 |
20120135433 | PROGNOSIS DIAGNOSIS METHOD AND PROGNOSIS DIAGNOSIS KIT FOR SEPSIS OR MULTIPLE ORGAN FAILURE - Disclosed are: a prognosis diagnosis method which can diagnose the prognosis of a patient suffering from sepsis or sepsis-related multiple organ failure in a simple manner and with high accuracy and a prognosis diagnosis kit for use in the prognosis diagnosis method. The prognosis diagnosis method comprises: a first detection step of detecting a liver fatty acid-binding protein contained in urine collected from a subject with a specific antibody; a second detection step of treating the urine with a Redox reagent such as hemin and detecting a liver fatty acid-binding protein contained in the treated urine with the specific antibody; and a comparison step of comparing a detection value obtained in the first step with a detection value obtained in the second step. It is determined that the larger the detection value obtained in the second step compared to that in the first step, the worse the prognosis. | 2012-05-31 |
20120135434 | MCPP IMMUNOASSAY - The invention describes a practical and robust multi-antibody approach to the sensitive immunodetection and determination of the drug of abuse m-chlorophenyl piperazine (mCPP). The invention also describes methods and kits for mCPP detection in an in vitro sample. | 2012-05-31 |
20120135435 | Methods for Identifying Factors That Control the Folding of Amyloid Proteins of Diverse Origin - The present invention provides a yeast cell based system for determining factors that control the folding of amyloid proteins of diverse origins. Further the present invention provides methods of using such a system to screen for reagents that affect amyloid formation, a process that is integral to several devastating human disease including Creutzfeld-Jacob disease (CJD), fatal familial insomnia (FFI), Gertsmann-Straussler-Scheinker (GSS) syndrome, and kuru. The system of the present invention provides a rapid screening system to quickly and cheaply identify reagents that affect the folding and aggregation properties of the target protein. | 2012-05-31 |
20120135436 | Method of Determining Enzymatic Activity in Biological Media - A reliable, fast and convenient method is provided to determine the change in concentration of enzyme in time in a biological medium in the presence of a signal substrate that corrects for substrate consumption, color-dependency of the signal and non-linearity between the concentration of the leaving group of the signal substrate and the amount of signal. The method comprises the measurement of a calibrator curve in a suitable medium, such as a buffer, to determine the characteristics of the measured curve. These characteristics will then allow to obtain this whole curve or a sufficient part thereof again through a mathematical procedure based on a single-point measurement in a sample of a medium in which the enzyme generation takes place. This has the great advantage that there is no need to measure the whole calibrator curve in each individual medium, since a single-point measurement is adequate. | 2012-05-31 |
20120135437 | BIOSENSORS UTILIZING INK JET-PRINTED BIOMOLECULE COMPATIBLE SOL GEL INKS AND USES THEREOF - Novel solid-phase biosensors that utilize ink jet printing of biocompatible sol-gel based inks to create sensor strips are reported herein. Biomolecules and other reagents useful in bioassays to detect, for example, pathogenic microorganisms or toxic substances, are immobilized on a substrate, which can be paper based, by layering these substances between two layers of biomolecule compatible sol gel. The sol gel precursor solutions and solutions of the assay reagents are printed from separate nozzles in a layered approach which avoids clogging of the nozzles by the pre-mature gelling of the sol gel precursor solution. In certain embodiments of the application, a capture agent is used to concentrate a compound to be detected in specific areas on the substrate to facilitate detection. | 2012-05-31 |
20120135438 | DETECTING A MICROORGANISM STRAIN IN A LIQUID SAMPLE - The invention concerns a medium for detecting, identifying and differentiating a microorganism strain in a liquid medium by contacting said liquid sample with a combination of chromogens substrates of enzymes expressed or not by the strain to be detected, the final coloration of the mixture being detectable in the wavelengths of the visible light. | 2012-05-31 |
20120135439 | METHOD FOR PRODUCTION OF ACCUMULATED PRODUCT OF NANO-SUBSTANCE, ACCUMULATED PRODUCT OF NANO-SUBSTANCE, DEVICE COMPRISING THE ACCUMULATED PRODUCT, AND METHOD FOR ANALYSIS OF STRUCTURE OF NANO-SUBSTANCE - A method for producing an accumulated product of a nano-substance that enables the accumulated product of the nano-substance to be produced at low cost, by a simple process that requires few conditions to be controlled and requires minimal energy, and with good reproducibility. Specifically, a method for producing an accumulated product of a nano-substance, the method including crystallizing a protein in a state where the protein and the nano-substance co-exist within a solvent, thereby accumulating the nano-substance within pores of the protein crystals to obtain the accumulated product of the nano-substance. | 2012-05-31 |
20120135440 | CATIONIC ANTI-MICROBIAL PEPTIDES AND METHODS OF USE THEREOF - Described herein are methods of detecting a wound infection and for detecting the presence or absence of microorganisms, for example, wound pathogens in a sample, by contacting a sample with a cationic anti-microbial peptide that is degradable by an enzyme produced and/or secreted by a microorganism, and detecting degradation or the absence of degradation of the peptide, as an indicator of the presence or absence of the enzyme in the sample, and thus indicative of the presence or absence of a microorganism in the sample. The present invention also features a biosensor for detecting the presence or absence of a microorganism in a sample. | 2012-05-31 |
20120135441 | ENZYMES AND METHODS FOR RESOLVING AMINO VINYL CYCLOPROPANE CARBOXYLIC ACID DERIVATIVES - Preparation and isolation of amino vinyl cyclopropane carboxylic acid derivatives and salts thereof, methods of resolving enantiomers, and methods of identifying compositions and/or enzymes that are capable of resolving racemic or partially enantiomerically enriched mixtures. | 2012-05-31 |
20120135442 | PROTEIN CRYSTALLIZATION USING MOLECULARLY IMPRINTED POLYMERS - The invention relates to a method comprising: providing a molecularly imprinted polymer imprinted with a first peptide or protein; exposing said molecularly imprinted polymer to a supersaturated solution of a second peptide or protein; and forming a nucleus of and/or growing a crystal of said second peptide or protein on said molecularly imprinted polymer. | 2012-05-31 |
20120135443 | Rapid Bed-Side Measurement of Neutrophil Elastase Activity in Biological Fluids - The subject invention provides novel devices and methods for the detection and quantification of neutrophil elastase activity in biological samples. | 2012-05-31 |
20120135444 | SAMPLING DEVICES AND METHODS OF USE - The present disclosure provides devices and rapid methods to acquire a wound sample to detect the presence of NO | 2012-05-31 |
20120135445 | CELL TRANSPORT SYSTEM - The present invention relates to a system for cell transport Said system allows the transport of cells, assuring their integrity and viability during the entire transport process. It consists of a system suitable for a wide variety of formats which allows a broad range of technical applications of the system The system of the invention allows providing ready-to-use cells, without the cells having to be manipulated before they are used by technical experts in cell biology The invention particularly relates to an agarose plus agarase mixture covering or enveloping, depending on the format of the selected transport system, the cell culture, protecting it during the transport process, as well as to the methodology of cell recovery of the cells transported in the system. | 2012-05-31 |
20120135446 | Microfluidic Device and Related Methods - A combinatorial microenvironment generator is configured for the generation of arbitrary, user-defined, steady-state, concentration gradients with negligible to no flow through the growth medium to perturb diffusion gradients or cellular growth. More importantly, the absolute concentrations and/or gradients can be dynamically altered upon request both spatially and temporally to impose tailored concentration fields for in-situ stimulus studies. Here, diffusion occurs via an array of ports, each of which can be an independently controlled source/sink. Together, the array of ports establishes a user-defined, 3D concentration profile. Useful methods related to this device are also provided. | 2012-05-31 |
20120135447 | NUCLEIC ACIDS ENCODING T2R, A NOVEL FAMILY OF TASTE RECEPTORS - The invention provides isolated nucleic acid and amino acid sequences of taste cell specific G-protein coupled receptors, antibodies to such receptors, methods of detecting such nucleic acids and receptors, and methods of screening for modulators of taste cell specific G-protein coupled receptors. | 2012-05-31 |
20120135448 | METHODS AND DEVICES FOR THE FABRICATION OF 3D POLYMERIC FIBERS - The present invention provides methods and devices for the fabrication of 3D polymeric fibers having micron, sub-micron, and nanometer dimensions, as well as methods of use of these polymeric fibers. | 2012-05-31 |
20120135449 | ITERATIVE STAINING OF BIOLOGICAL SAMPLES - Automated methods and devices that facilitate iterative staining of biological samples from imaging applications are provided. The methods include the steps of providing a small volume flow cell containing a biological sample, applying a stain to the biological sample, combining at least two precursor reagents to form an activated destaining agent and wherein the activated destaining agent decomposition rate is greater than or similar to the destaining reaction rate, flowing the destaining agent over the biological sample at a flow rate that is greater than the decomposition rate of the activated destaining agent, and releasing the sample from the flow cell wherein the integrity of the sample is intact. The process of staining, combining and flowing may be iteratively repeated. Also disclosed herein are devices for iterative staining of biological samples comprising a flow cell, in fluid communication with a premixer, wherein the volume capacity of the premixer is smaller than about five times the volume capacity of the flow cell. | 2012-05-31 |
20120135450 | SUBSTRATES, DEVICES, AND METHODS FOR QUANTITATIVE LIQUID CRYSTAL ASSAYS - The present invention relates to the field of molecular diagnostics, and in particular to diagnostics based on a liquid crystal assay format. In particular, the present invention provided improved substrates and methods of using liquid crystal assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal assay format. | 2012-05-31 |
20120135451 | Method and Apparatus for Evaluation of Toxicity - The present invention pertains to a method for evaluation of the toxicity of compounds comprising the use of spermatozoa. The inventions also relates to a high throughput system for the toxicity evaluation. | 2012-05-31 |
20120135452 | MICROFLUIDIC DEVICE FOR PHARMACOKINETIC-PHARMACODYNAMIC STUDY OF DRUGS AND USES THEREOF - A microfluidic device for culturing cells, termed a microscale cell culture analog (μCCA), is provided. The microfluidic device allows multiple cell or tissue types to be cultured in a physiologically relevant environment, facilitates high-throughput operation and can be used for drug discovery. The microfluidic device uses gravity-induced fluidic flow, eliminating the need for a pump and preventing formation of air bubbles. Reciprocating motion between a pair of connected reservoirs is used to effect the gravity-induced flow in microfluidic channels. Bacterial contamination is reduced and high throughput enabled by eliminating a pump. The microfluidic device integrates a pharmacokinetic-pharmacodynamic (PK-PD) model to enable PK-PD analyses on-chip. This combined in vitro/in silico system enables prediction of drug toxicity in a more realistic manner than conventional in vitro systems. | 2012-05-31 |
20120135453 | Methods of Treatment Using CCA1 Inhibitors - Provided herein are methods for the treatment or prevention of a fungal infection in a host comprising the administration to the host a therapeutically or prophylactically effective amount of a CCA1 inhibitor. | 2012-05-31 |
20120135454 | METHODS FOR SEPARATION, CHARACTERIZATION AND/OR IDENTIFICATION OF MICROORGANISMS USING SPECTROSCOPY - The present invention is directed to a method for separating, characterizing and/or identifying microorganisms in a test sample. The method of the invention comprises an optional lysis step for lysing non-microorganism cells that may be present in a test sample, followed by a subsequent separation step. The method may be useful for the separation, characterization and/or identification of microorganisms from complex samples such as blood-containing culture media. The method may also be useful for the physical separation and/or enrichment of two or more different or individual microorganism species contained in a mixed test sample. The invention further provides for spectroscopic interrogation of the separated microorganism sample(s) to produce measurements of the microorganism and characterizing and/or identifying the microorganism(s) in the sample using said spectroscopic measurements. | 2012-05-31 |
20120135455 | Smart Packaging For Detecting Microorganisms - The present invention relates to novel smart packaging, designed using a novel material comprising a partially polar adsorbent solid base impregnated with a solution of vanillin, which allows the growth of microorganisms in different types of products to be detected visually without having to be in direct contact with the microorganism or with the medium containing same. | 2012-05-31 |
20120135456 | METHOD AND KIT FOR DETECTION OF GUAIACOL-PRODUCING BACTERIUM - Disclosed is a method for detecting guaiacol-producing bacteria in a specimen comprising culturing the specimen or a dilution thereof on a plate of a solid medium for acidophilic bacteria comprising a compound represented by the following formula, wherein R is —H, —OH, —C(O)H, —C(O)CH | 2012-05-31 |
20120135457 | APPARATUS AND METHOD FOR THE DETECTION AND ANALYSIS OF PARTICLES IN FLUIDS - The invention relates to apparatus for the detection of particles and for particle analysis. The apparatus comprises a sample holder comprising a base and a projection extending from the base. The base includes a contact region where, in use, the surface of a fluid sample may contact the projection. The surface of at least the contact region of the projection exhibits properties that allow the surface of the contact region to be substantially wetted by a fluid sample when the apparatus is in use so that the fluid sample forms a meniscus having its apex in contact with the contact region of the projection. The invention also relates to methods for using the apparatus. | 2012-05-31 |
20120135458 | CLOSED LOOP MONITORING OF AUTOMATED MOLECULAR PATHOLOGY SYSTEM - A closed loop automated method for staining of a biological sample is provided. The method comprises providing a biological sample, staining at least a portion of the biological sample by flowing in a reagent, monitoring one or more optical characteristics of the biological sample, and calculating a figure of merit based on at least one of the optical characteristics. An automated device for iterative staining of a biological sample is also provided. | 2012-05-31 |
20120135459 | NOVEL FLUORINATED RHODAMINES AS PHOTOSTABLE FLUORESCENT DYES FOR LABELLING AND IMAGING TECHNIQUES - The present invention relates to novel fluorinated | 2012-05-31 |
20120135460 | RECOMBINANT PORCINE CHYMOTRYPSIN - The present invention generally relates to the field of proteinases and more specifically to chymotrypsin. In particular, the present invention relates to recombinant porcine chymotrypsin and its use in food applications. | 2012-05-31 |
20120135461 | PRODUCTION OF GLYCOPROTEINS WITH REDUCED O-GLYCOSYLATION COMPRISING THE USE OF AN ALPHA-1,2-MANNOSIDASE - A method is described for producing protein compositions having reduced amounts of O-linked glycosylation. The method includes producing the protein in cells cultured in the presence of one or more α-1,2-mannosidases from | 2012-05-31 |
20120135462 | Polymer-Von Willebrand Factor-Conjugates - The present invention relates to a proteinaceous construct (also designated as polymer-VWF-conjugate) comprising plasmatic and/or recombinant von Willebrand factor (VWF), said VWF being bound to at least one physiologically acceptable polymer molecule, as well as to a complex between said proteinaceous construct and at least one factor VIII (FVIII) protein. The physiologically acceptable polymer molecule can be, for instance, polyethylene glycol (PEG) or polysialic acid (PSA). Further the present invention relates to methods for prolonging the in vivo-half-life of VWF or FVIII in the blood of a mammal having a bleeding disorder associated with functional defects of or deficiencies of at least one of FVIII or VWF. | 2012-05-31 |
20120135463 | Rhamnose promoter expression system - Vectors expressible in a host that is the rhaBAD promoter region of the L-rhamnose operon operably linked to a transcriptional unit that is:
| 2012-05-31 |
20120135464 | STIRRER SYSTEM - The present invention concerns a stirrer system for animal cell culture consisting of a combination of at least one radially-conveying stirrer element and at least one axially-conveying stirrer element, wherein at least three stirrer elements must be present and the uppermost stirrer element is an axially-conveying stirrer element. The stirrer elements are arranged at a certain distance above one another on a stirrer shaft. A particular embodiment is a multiple stirrer system consisting of two disk stirrers as radially-conveying stirrer elements and an inclined-blade stirrer as an axially-conveying stirrer element wherein the inclined-blade stirrer is arranged above the disk stirrer on the stirrer shaft. The stirrer system according to the invention achieves among others a gentler and better intermixing in the culture of shear-sensitive mammalian cells in cell cultures. | 2012-05-31 |
20120135465 | Endoglucanase For Reducing The Viscosity Of A Plant Materials Slurry - The present disclosure relates to composition comprising EG cellulase and methods of use, thereof. The compositions are useful, e.g., for reducing the viscosity of plant material slurry. | 2012-05-31 |
20120135466 | Production Of Maltotetraose Syrup Using A Pseudomonas Saccharophila Maltotetraohydrolase Variant And A Debranching Enzyme - The combination of a | 2012-05-31 |
20120135467 | HMO SYNTHESIS - The present invention relates to a cell to be stably cultured in a medium, which cell is adjusted for the production of oligosaccharides, the cell being transformed to comprise at least one nucleic acid sequence coding for an enzyme involved in oligosaccharide synthesis. In addition the cell is transformed to comprise at least one nucleic acid sequence coding for a protein of the sugar efflux transporter family, a functional homolog or derivative thereof. Further, the invention concerns a method for the production of oligosaccharides involving above cell. | 2012-05-31 |
20120135468 | BETA-GALACTOSIDASE DERIVED FROM BACILLUS CIRCULANS - Disclosed is a novel β-galactosidase. Specifically disclosed are a β-galactosidase derived from | 2012-05-31 |
20120135469 | NOVEL GLYCOSYLTRANSFERASE, NOVEL GLYCOSYLTRANSFERASE GENE, AND NOVEL SUGAR DONOR COMPOUND - An object of the present invention is to provide a sugar donating reagent comprising a sugar donor compound other than a sugar nucleotide and an enzyme capable of catalyzing a glycosyl transfer reaction using a sugar donor compound other than a sugar nucleotide. The present invention provides the following: a sugar donating reagent containing a compound of formula (A): | 2012-05-31 |
20120135470 | BIOTECHNOLOGICAL PRODUCTION OF CHONDROITIN - An innovative method is described for the production of chondroitin at high concentration, by fermentation of genetically mutated bacteria. | 2012-05-31 |
20120135471 | Method for Concentrating Sample Constituents and Amplifying Nucleic Acids - A method is disclosed for concentrating sample constituents and for multiplying nucleic acids from a biological sample which are containing in the sample constituents. The nucleic acids are amplified on the same filter on which the sample constituents are also separated off. | 2012-05-31 |
20120135472 | HOT-START PCR BASED ON THE PROTEIN TRANS-SPLICING OF NANOARCHAEUM EQUITANS DNA POLYMERASE - Disclosed is a hot-start PCR method, based on protein trans-splicing of intein-inserted large (Neq L) and small (Neq S) fragments of Neq DNA polymerase. The method comprises: preparing a PCR reaction mixture containing a sample DNA and primers; adding the Neq L fragment and the Neq S fragment together to the PCR reaction mixture, said Neq L fragment consisting of an amino acid sequence of SEQ ID NO: 2, with an intein amino acid sequence stretching from position 579 to 676 therein, said Neq S fragment consisting of an amino acid sequence of SEQ ID NO: 4 with an intein amino acid sequence stretching from position 1 to 30 therein; inducing the Neq L fragment and the Neq S fragment to undergo a protein trans-splicing process to form a polypeptide exhibiting Neq DNA polymerase activity; and performing a certain number of cycles of DNA denaturation, primer annealing and DNA extension. | 2012-05-31 |
20120135473 | PROCESSES USING DUAL SPECIFICITY OLIGONUCLEOTIDE AND DUAL SPECIFICITY OLIGONUCLEOTIDE - The present invention relates to various processes by a template-dependent extension reaction using a dual specificity oligonucleotide and a dual specificity oligonucleotide composed of three different Tm portions therefor. Demonstrated in the present invention are the features of the dual specificity oligonucleotide, which are high hybridization specificity and mismatch tolerance. | 2012-05-31 |
20120135474 | HEMICELLULOSE-DEGRADING ENZYMES - The present disclosure provides methods for the conversion of hemicellulose into fermentable sugars using enzymes isolated from | 2012-05-31 |
20120135475 | LIQUEFIED BIOMASS - The invention is directed to a liquefied sugar beet and/or sugar cane biomass material as well as production methods and uses thereof. The liquefied biomass is storage stable and can be used for the production of a product resulting from fermentation. | 2012-05-31 |
20120135476 | PROCESS FOR PREPARING N-HETEROCYCLIC OPTICALLY ACTIVE ALCOHOLS - Process for preparing N-heterocyclic optically active alcohols of the formula I | 2012-05-31 |
20120135477 | Biocatalytic Production of Ambroxan - The invention relates to a process for the biocatalytic production of ambroxan by means of a polypeptide with the activity of a homofarnesol-ambroxan cyclase, which are a novel class of enzymes. | 2012-05-31 |
20120135478 | ALGAL MEDIUM CHAIN LENGTH FATTY ACIDS AND HYDROCARBONS - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. More specifically, the invention relates to a | 2012-05-31 |
20120135479 | FRACTIONATION OF OIL-BEARING MICROBIAL BIOMASS - The invention generally relates to the production of hydrocarbon compositions, such as a lipid, in microorganisms. In particular, the invention provides methods for extracting, recovering, isolating and obtaining a lipid from a microorganism and compositions comprising the lipid. The invention also discloses methods for producing hydrocarbon compositions for use as biodiesel, renewable diesel, jet fuel, and other materials. | 2012-05-31 |
20120135480 | METHODS FOR PRODUCING POLYHYDROXYALKANOATES FROM BIODIESEL-GLYCEROL - Methods are provided for producing biodegradable polyhydroxyalkanoates (PHAs) with desired geometry, molecular mass, mechanical and/or physical-chemical properties from glycerol, an inexpensive carbon source and byproduct of the biodiesel industry. Microorganisms capable of converting carbon to PHA can be used to convert biodiesel-glycerol to poly-3-hydroxybutyrate (PHB) or other monomer or copolymer PHAs via fermentation. The microorganisms are cultured in a medium comprising glycerol as a primary carbon source and one or more low molecular mass organic acids as a secondary carbon source. Biomass can be harvested from the culture medium and crude PHA extracted and purified, thereby recovering purified PHA with the desired property. After PHA isolation, a nucleating agent can be added to improve certain physical-chemical properties of the PHA; e.g., crystallization temperature, to enhance performance of the PHA during injection molding. | 2012-05-31 |
20120135481 | Compositions and methods for 3-hydroxypropionic acid production - The present application discloses genetically modified yeast cells comprising an active 3-HP fermentation pathway, and the use of these cells to produce 3-HP. | 2012-05-31 |
20120135482 | DICARBOXYLIC ACID FERMENTATION PROCESS - The present invention relates to a process for producing a dicarboxylic acid, comprising fermenting a recombinant fungal cell in a suitable fermentation medium, in the presence of high carbon dioxide concentrations. | 2012-05-31 |
20120135483 | Preparation of Isomerically Pure Substituted Cyclohexanols - The present invention relates to the preparation of isomerically pure substituted cyclohexanols starting from a mixture of cis/trans substituted cyclohexanols which comprises reacting the cis/trans mixture of a substituted cyclohexanol with a dicarboxylic acid anhydride in the presence of a lipase, to give the trans semi-ester which is separated off from the unreacted substituted cyclohexanol cis isomer. | 2012-05-31 |
20120135484 | METHOD FOR PRODUCING MICROBIAL FERMENTATION PRODUCT - Disclosed is a method for producing 1-(2-hydroxyethyl) 2,5,5,8a-tetramethyldecahydronaphthalene-2-ol represented by formula (2), | 2012-05-31 |
20120135485 | SORBENT AND PROCESS FOR REMOVING FERMENTATION INHIBITORS - The invention pertains to, for example, an improved sorbent and process for removing fermentation inhibitors such as furfural and/or HMF in microbial processes utilizing fermentable sugars obtained from biomass including, for example, in the production of bioalcohols. The sorbent is capable of separating one or more inhibitors from monosaccharides and is characterized by: (1) a K | 2012-05-31 |
20120135486 | Methods and Compositions for the Extracellular Transport of Biosynthetic Hydrocarbons and Other Molecules - The present disclosure identifies methods and compositions for modifying photoautotrophic organisms as hosts, such that the organisms efficiently convert carbon dioxide and light into hydrocarbons, e.g., n-alkanes and n-alkenes, wherein the n-alkanes are secreted into the culture medium via recombinantly expressed transporter proteins. In particular, the use of such organisms for the commercial production of n-alkanes and related molecules is contemplated. | 2012-05-31 |
20120135487 | MUTANT GLYCEROL DEHYDROGENASE (GLYDH) FOR THE PRODUCTION OF A BIOCHEMICAL BY FERMENTATION - The present invention concerns a method for the production of 1,2-propanediol, comprising culturing a microorganism modified for an improved production of 1,2-propanediol in an appropriate culture medium and recovery of the 1,2-propanediol which may be further purified wherein the microorganism expresses a glycerol dehydrogenase (GlyDH) enzyme the inhibition of which activity by NAD+ and/or its substrate and/or its product is reduced. | 2012-05-31 |
20120135488 | METHODS FOR THE PREPARATION AND USE OF CELLULOSIC FEEDSTOCK FOR ETHANOL PRODUCTION - The instant invention provides methods for increasing the efficiency and yield of cellulosic ethanol production. | 2012-05-31 |
20120135489 | METHOD OF ALCOHOL PRODUCTION - The present invention relates to a process for producing alcohol from a cellulosic material, said process comprising: hydrolyzing said cellulosic material with an aqueous acid to produce a hydrolysate; extracting acid and water from said hydrolysate with a water-miscible organic extraction solvent to yield (a) a first aqueous acidic solution containing said extraction solvent and (b) a residue containing sugars; subjecting said residue to an oligosaccharide cleavage reaction to yield an aqueous solution of fermentable sugars; fermenting said fermentable sugars and distilling alcohol from the resulting fermented mixture; contacting said first aqueous acidic solution with a water-immiscible liquid lipophilic solvent to yield a second aqueous acid solution and a solvent mixture of said extraction solvent and said liquid solvent; separating said solvent mixture to yield extraction solvent for recycling; and separating from said second aqueous acid solution an aqueous acid for recycling. | 2012-05-31 |
20120135490 | ISOPRENE HYDROCARBON PRODUCTION USING GENETICALLY ENGINEERED CYANOBACTERIA - The present invention provides methods and compositions for producing isoprene hydrocarbons from cyanobacteria. | 2012-05-31 |
20120135491 | METHOD FOR PRODUCING BIOGAS OR SEWAGE GAS - The invention relates to a method for producing biogas or sewage gas by a multi-stage anaerobic reaction of biomass and/or sludge. Considering the disadvantages of the known prior art, a method is to be provided that leads to a higher yield of raw gas or biogas and a higher content of methane in the raw gas and enables an economically improved operating method. To this end, the reaction is carried out in the first fermentation stage (F | 2012-05-31 |
20120135492 | APPARATUS AND METHOD OF USING AN AGRICULTURAL WASTE DIGESTER AND BIOGAS GENERATION SYSTEM - An agricultural waste digester and biogas generation system. The system includes a digester assembly having a cylindrical body, a hollow interior, a center axis and a plurality of wheel segments within the interior of the digester assembly. A gas conduit extends from the interior of the digester assembly to a power generation device. Also included is a water vessel containing water, and each of the plurality of wheel segments have an acruate, contoured surface area which restrict biogas movement within the digester assembly to produce induced agitation of agricultural waste. | 2012-05-31 |
20120135493 | Methods for Phototransfecting Nucleic Acids Into Live Cells - The present invention includes methods for transferring a multigenic phenotype to a cell by transfecting, preferably by phototransfection, and locally transfecting a cell or a cellular process with a laser while the cell is bathed in a fluid medium comprising two or more nucleic acids, thereby introducing the nucleic acid into the interior of the cell. Expression of the nucleic acids results in a multigenic phenotype in the tranfected cell. | 2012-05-31 |
20120135494 | SYSTEMS AND METHODS FOR MAGNETIC SEPARATION OF BIOLOGICAL MATERIALS - A magnetic separator comprising a separation chamber is provided. The magnetic separator comprises an inlet and at least one outlet, and a magnetic source operatively coupled to the separation chamber and comprising a plurality of magnets that can be selectively turned off and on to create a dynamic magnetic field in the separation chamber. | 2012-05-31 |
20120135495 | NEUROTOXINS WITH ENHANCED TARGET SPECIFICITY - Modified neurotoxins that contain protease cleavage sites susceptible uniquely to proteases present in certain tissues are described. The toxins can be selectively activated by proteases in muscle or selectively inactivated by proteases in blood. | 2012-05-31 |
20120135496 | Protein Belonging to the TNF Superfamily Involved in Signal Transduction, Nucleic Acids Encoding Same and Methods of Use Thereof - A method of modulating immune response in an animal is disclosed. Such a method interacting the immature dendritic cells from the animal with an antigen ex vivo so that the immature dendritic cells present the antigen on their surfaces, inducing maturation of the immature dendritic cells ex vivo, and contacting the mature dendritic cells ex vivo with a modulator comprising TRANCE, conservative variants thereof, fragments thereof, analogs or derivatives thereof, or a fusion protein comprising the amino acid sequence of TRANCE, conservative variants thereof, or fragments thereof. After contacting the modulator ex vivo, the mature dendritic cells are introduced into the animal. As a result, immune response in the animal towards the antigen is modulated relative to the immune response against the antigen in an animal in which dendritic cells did not interact with the antigen ex vivo, and did not contact a modulator ex vivo. Preferably, the method of the present invention results in increasing immune response towards the antigen in the animal. | 2012-05-31 |
20120135497 | DIFFERENTIALLY FLUORESCENT YEAST BIOSENSORS FOR THE DETECTION AND BIODEGRADATION OF CHEMICAL AGENTS - The present disclosure provides methods, devices, systems and compositions for detecting and/or modifying chemical agents. In some embodiments, a biosensor may be configured to detect a chemical agent, modify that agent to a form with reduced toxicity, and/or detect the modified form of the chemical agent. The present disclosure also relates, in some embodiments, to variant organophosphorus hydrolase having one or more desirable amino acid substitutions. | 2012-05-31 |
20120135498 | Method for Producing Nucleases of a Gram Negative Bacterium While Using a Gram Positive Expression Host - A method for producing a nuclease of a gram negative bacterium or a nuclease preparation containing a nuclease of a gram negative bacterium including expression of the nuclease in a gram positive bacterium and subsequent secretion of the nuclease, as well as a nuclease or a nuclease preparation that can be obtained by this method. | 2012-05-31 |
20120135499 | EXO-ENDO CELLULASE FUSION PROTEIN - The present invention relates to a heterologous exo-endo cellulase fusion construct, which encodes a fusion protein having cellulolytic activity comprising a catalytic domain derived from a fungal exo-cellobiohydrolase and a catalytic domain derived from an endoglucanase. The invention also relates to vectors and fungal host cells comprising the heterologous exo-endo cellulase fusion construct as well as methods for producing a cellulase fusion protein and enzymatic cellulase compositions. | 2012-05-31 |
20120135500 | CELLULASE VARIANTS WITH IMPROVED EXPRESSION, ACTIVITY AND STABILITY, AND USE THEREOF - The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof. | 2012-05-31 |
20120135501 | Recombinant poxvirus expressing homologous genes inserted - The present invention relates to a recombinant poxvirus vector capable of expressing two or more homologous, foreign sequences, which derive from different variants of a microorganism, and which have a homology of 50% or above. The invention further relates to a method for preparing such recombinant poxvirus and the use of such recombinant poxvirus as medicament or vaccine. Additionally, a method for affecting preferably inducing, an immune response in a living animal, including a human, is provided. | 2012-05-31 |
20120135502 | NOVEL SELF-DYING RECOMBINANT MICROORGANISM FOR MEASURING SOIL AND WATER CONTAMINATION - Provided is | 2012-05-31 |
20120135503 | MINICELL BASED DELIVERY OF BIOLOGICALLY ACTIVE COMPOUNDS - Minicells are used to deliver biologically active compounds including radioisotopes, polypeptides, nucleic acids, small molecules, drug molecules, and chemotherapeutic agents. In some cases, the minicell displays ligands or binding moieties that target the minicell to a desired host cell. | 2012-05-31 |
20120135504 | Method for Producing Fungus Structures - A method for growing organically derived building materials in the form of a moldable substrate that can be engineered to serve a wide range of manufacturing and construction applications is presented. In particular, the embodiments consider a plurality of fungal molded shapes preferably grown from fungal inoculum and mechanically compressed at least once during the growing process as well as integration of structure support members to the fungal structure. The present invention provides a fungal substrate which could be molded, and easily and cheaply preprocessed to precise geometric specifications. The organically derived building materials also incorporate layers of structural reinforcements to improve load bearing and other structural capacities. | 2012-05-31 |
20120135505 | METHOD FOR PREPARING MICROCRYSTALLINE CELLULOSE - The present invention relates to a new method for preparing microcrystalline cellulose, comprising a prior step of compaction of the cellulose before degradation of the glucose chains and obtaining the suitable degree of polymerization. It is also described that the method of the invention allows a considerable reduction in the consumption of energy, water and possible chemicals which are used for reducing the degree of polymerization. The microcrystalline cellulose obtained can be used as a pharmaceutical excipient in tablets, and presents disintegration features comparable to those of the microcrystalline cellulose obtained by a spraying process. | 2012-05-31 |
20120135506 | PROCESS FOR PRODUCING MICROFIBRILLATED CELLULOSE - A process for treating cellulosic fibres comprising pre-treating the fibres with an enzyme in a first enzymatic treatment followed by mechanical pre-treating the fibres in a first mechanical treatment and a second enzymatic treatment followed by a second mechanical treatment of the fibres to form micofibrillated cellulose. In this way it is possible to produce mircofibrillated cellulose (MFC) in an improved and energy efficient way. | 2012-05-31 |
20120135507 | BIOCHIP MODULE WITH CERAMIC LAMINATE STRUCTURE AND METHOD OF MANUFACTURING THE SAME - The present disclosure provides a biochip module having a ceramic laminate structure which uses advantages of ceramic and enables a reduction in a chip area, and a method of manufacturing the same. The biochip module includes a first ceramic layer mixing bacterial water with magnetic beads to which ligands capturing bacteria are attached, a second ceramic layer separating the magnetic beads capturing bacteria from the water, and a third ceramic layer detecting the number of bacteria captured by the magnetic beads. | 2012-05-31 |
20120135508 | SYSTEM AND METHOD FOR ANALYTE MEASUREMENT - A method of measuring an analyte in a biological fluid comprises applying an excitation signal having a DC component and an AC component. The AC and DC responses are measured; a corrected DC response is determined using the AC response; and a concentration of the analyte is determined based upon the corrected DC response. Other methods and devices are disclosed. | 2012-05-31 |
20120135509 | SENSOR DEVICES - The present invention relates to a sensor device for measuring a level of an analyte of interest in a fluid, such as body fluid (for example blood, plasma, urine, interstitial fluid, saliva), a method of manufacturing a sensor device, a reagent film for use in a sensor, a method of manufacturing a reagent film for use in a sensor, a method of conducting an assay using a sensor, a method of calibrating a measurement from a sensor, a method of calibrating a batch of sensors, a meter for use with a sensor and a kit comprising a meter and a sensor according to the invention. A first aspect of the invention provides a sensor device for measuring a level of an analyte of interest In a fluid comprising: a flowpath for the fluid; on the flowpath, a reagent for the analyte of interest adjacent to an internal standard comprising a first predetermined amount of a first calibration analyte; and further wherein the reagent and the predetermined amount of a first calibration analyte are in dry form. A second aspect of the invention provides a device comprising: a first calibration electrode having the first predetermined amount of first calibration analyte and reagent for the analyte of interest located thereon; a first working electrode having reagent for the analyte of interest thereon, and further wherein the first calibration electrode lies upstream of the first working electrode. | 2012-05-31 |
20120135510 | Electrokinetic Device for Capturing Assayable Agents in a Dielectric Fluid - Electrokinetic devices and methods are described with the purpose of collecting assayable agents from a dielectric fluid medium. Electrokinetic flow may be induced by the use of plasma generation at high voltage electrodes and consequent transport of charged particles in an electric voltage gradient. In one embodiment, the agents are directed electrokinetically to the sample collection assay device with no intermediate transfer steps. The agents are directed by creation of an electrokinetic potential well, which will effect their capture on to an assay device. Environmental agents such as biowarfare agents, pathogens, allergens or pollutants are collected autonomously on to the assay device, without any human intervention. The dielectric fluid medium, such as air, is sampled by electrokinetic propulsion with no moving parts or optionally, by transporting the dielectric fluid by a fan, pump or by breath. A further embodiment for collection of pathogen samples entails breathing into a tube where the sample is exposed to an electric plasma in the neighborhood of a high voltage electrode or electrodes, further transported by the breath through a potential well created at a sample collection device, where charge particles are electroprecipitated. The dielectric fluid medium may further include non-conductive liquids, such as oils. Oils may be sampled for the presence of contaminants, contaminating organisms or bio-degrading organisms. | 2012-05-31 |
20120135511 | PORTABLE HIGH GAIN FLUORESCENCE DETECTION SYSTEM - Disclosed is a compact, microprocessor-controlled instrument for fluorometric assays in liquid samples, the instrument having a floating stage with docking bay for receiving a microfluidic cartridge and a scanning detector head with on-board embedded microprocessor for controlling source LEDs, emission signal amplification and filtering in an isolated, low noise, high gain environment within the detector head. Multiple optical channels may be incorporated in the scanning head. In a preferred configuration, the assay is validated using dual channel optics for monitoring a first fluorophore associated with a target analyte and a second fluorophore associated with a control. Applications include molecular biological assays based on PCR amplification of target nucleic acids and fluorometric assays in general, many of which require temperature control during detection. Sensitivity and resistance to bubble interference during scanning are shown to be improved by use of a heating block with reflective mirror face in intimate contact with a thermo-optical window enclosing the liquid sample. | 2012-05-31 |
20120135512 | LIGHT TRAPPING OPTICAL COVER - A light trapping optical cover employing an optically transparent layer with a plurality of light deflecting elements. The transparent layer is configured for an unimpeded light passage through its body and has a broad light input surface and an opposing broad light output surface. The light deflecting elements deflect light incident into the transparent layer at a sufficiently high bend angle with respect to a surface normal and direct the deflected light toward a light harvesting device adjacent to the light output surface. The deflected light is retained by means of at least TIR in the system formed by the optical cover and the light harvesting device which allows for longer light propagation paths through the photoabsorptive layer of the device and for an improved light absorption. The optical cover may further employ a focusing array of light collectors being pairwise associated with the respective light deflecting elements. | 2012-05-31 |
20120135513 | Bioreactor consisting of silicone materials - The invention relates to a bioreactor for cultivating phototrophic organisms in an aqueous culture medium, the reactor parts that come into contact with the culture medium being entirely or partially produced from silicone materials. The invention is characterized in that the silicone materials are produced from addition-crosslinked silicones, and the surface of the silicone materials has a contact angle to the water of at least 100°. | 2012-05-31 |
20120135514 | Bioreactor Comprising A Silicone Coating - The invention relates to a bioreactor for cultivating phototrophic organisms in an aqueous culture medium. The reactor parts and/or fittings that come into contact with the culture medium are entirely or partially coated with a silicone layer, and the surface of the silicone layer has a contact angle to the water of at least 100°. | 2012-05-31 |
20120135515 | METHODS FOR PRODUCING PREPARATIONS OF RECOMBINANT AAV VIRIONS SUBSTANTIALLY FREE OF EMPTY CAPSIDS - Methods for separating AAV empty capsids from mixtures of AAV vector particles and AAV empty capsids are described. The methods use column chromatography techniques and provide for commercially viable levels of recombinant AAV virions. | 2012-05-31 |
20120135516 | ARTIFICIAL ANTIBODY POLYPEPTIDES - A fibronectin type III (Fn3) polypeptide monobody, a nucleic acid molecule encoding said monobody, and a variegated nucleic acid library encoding said monobody, are provided by the invention. Also provided are methods of preparing a Fn3 polypeptide monobody, and kits to perform said methods. Further provided is a method of identifying the amino acid sequence of a polypeptide molecule capable of binding to a specific binding partner (SBP) so as to form a polypeptide:SSP complex, and a method of identifying the amino acid sequence of a polypeptide molecule capable of catalyzing a chemical reaction with a catalyzed rate constant, k | 2012-05-31 |
20120135517 | SYNTHETIC GENES AND GENETIC CONSTRUCTS - The present invention relates generally to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention provides novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto. | 2012-05-31 |
20120135518 | DEFINED SURFACES OF SELF-ASSEMBLED MONOLAYERS AND STEM CELLS - A method for the construction of arrays from self-assembling monolayers is described. The arrays have particular utility for the screening of peptides ligands that can foster the growth of cells in culture. This technique has been used to identify peptide ligands that foster the growth of human stem cells, which otherwise require an extracellular matrix in order to grow in an undifferentiated state. This also makes possible an assay to identify other such peptides. | 2012-05-31 |
20120135519 | INDUCED DERIVATION OF SPECIFIC ENDODERM FROM hPS CELL-DERIVED DEFINITIVE ENDODERM - The present invention relates to a method to control differentiation of human pluripotent stem cells, including human balstocyst derived stem (hBS) cells and to obtain specific endoderm cells. In particular, present invention relates to the use of FGF2 as the key factor in a specific concentration to control differentiation of definitive endoderm cells derived from hPS cells to specific endoderm cells. The invention also provides methods of obtaining endoderm cells comprising the use of FGFR and activation of the MAPK signalling pathway. | 2012-05-31 |