21st week of 2013 patent applcation highlights part 51 |
Patent application number | Title | Published |
20130130285 | Markers for Renal Disease - This invention provides reagents and methods for diagnosing renal disease. Differential levels of inosine metabolite, and proteins: apolipoprotein C-I, apolipoprotein C-II, fibrinogen alpha chain, or fibrinogen A-alpha chain, kininogen, Inter-Alpha Inhibitor H4 (ITIH4), keratin Type I cytoskeletol 10 cystatin A, cystatin B and other polypeptides and fragments thereof provide biomarkers of renal disease and are described herein. | 2013-05-23 |
20130130286 | METHODS FOR ASSESSING THE IMMUNE SYSTEM IN A PATIENT - Methods of determining the onset or susceptibility of an immunological disease are provided herein. Also provided are immunoassay techniques for carrying out such methods. | 2013-05-23 |
20130130287 | MARKERS FOR ACUTE KIDNEY INJURY - Provided are methods and compositions for predicting the development of kidney disease, including acute kidney injury. In certain aspects and embodiments the provided methods and compositions are particularly useful for predicting kidney injury following an event likely to cause kidney injury and/or kidney failure in a patient, such as a cardiac surgery, e.g., a surgery involving a cardiopulmonary bypass (CPB), such as a coronary artery bypass graft surgery. In some embodiments, the higher the urinary hepcidin-to-urinary creatinine ratio (uHep/uCr) at 6-24 hours following initiation of CPB, the lower is the risk for development of AKI determined by RIFLE criteria in the ensuing four to five days. Conversely, the higher the urinary NGAL to urinary creatinine ratio (uNGAL/uCr) at 6-24 hours following initiation of CPB, the higher is the risk of developing CPB-mediated AKI over the same time period. | 2013-05-23 |
20130130288 | Antibodies, Kit and Method for Detecting Amyloid Beta Oligomers - This invention is a selective Aβ oligomer kit and immunoassay method capable of reliably and sensitively detecting Aβ oligomers in a biological sample of a patient. In one embodiment the inventive assay uses a pair of anti-Aβ oligomer antibodies, as capture and detection antibodies, to detect and quantify Aβ oligomers. The method can be used to differentiate Alzheimer's disease (AD) patients from non-AD patients and/or to stratify AD patients according to the severity of their disease. | 2013-05-23 |
20130130289 | COMPOUNDS AND METHODS FOR ASSAYING REDOX STATE OF METABOLICALLY ACTIVE CELLS AND METHODS FOR MEASURING NAD(P)/NAD(P)H - The present invention provides compounds and methods for assaying redox state of metabolically active cells and methods for assaying enzyme activity and/or metabolite level by coupling to redox defining co-factor NAD(P)/NAD(P)H measurement. | 2013-05-23 |
20130130290 | DISTINGUISHING CELLS IN A SAMPLE BY INACTIVATING EXTRACELLULAR ENZYME BEFORE RELEASING INTRACELLULAR ENZYME - A method for detecting the absence or presence of cells of interest in a liquid sample, wherein: (a) the sample: (i) comprises an extracellular medium containing an enzyme with a measurable activity; and (ii) is suspected of containing cells of interest that contain an enzyme with said measurable activity; and (b) the method comprises the steps of: (i) treating the liquid sample with a reagent that inactivates said measurable activity in the extracellular medium, but does not inactivate the measurable activity in said cells of interest; (ii) lysing the cells of interest to release the intracellular enzyme; and (iii) measuring said measurable activity. Thus the intracellular enzyme can be measured without interference from the extracellular enzyme. The invention is particularly useful for treatment of bacterially-infected blood using a detection assay based on adenylate kinase activity. | 2013-05-23 |
20130130291 | Labelling of Fusion Proteins with Synthetic Probes - The invention relates to new proteins called alkylcytosine transferases (ACTs) derived from O | 2013-05-23 |
20130130292 | Method for detecting replication or colonization of a biological therapeutic - Methods for detecting replication in or colonization of a host by a biological therapeutic, such as an oncolytic virus, cells administered for cell therapy and gene therapy vectors, are provided. In the methods, a product produced by the biological therapeutic is detected in a sample of tissue or body fluid distinct from the administered therapy or locus thereof, thereby permitting assessment of the therapy and/or monitoring its progress. | 2013-05-23 |
20130130293 | Method for Reducing Star Activity in Restriction Endonucleases - Methods are provided for making restriction endonucleases with reduced star activity by one or more targeted mutations to a catalytic site within the restriction endonuclease. Examples of modifications to restriction endonucleases with significant sequence identity with KpnI are provided and reduced star activity demonstrated. | 2013-05-23 |
20130130294 | NOVEL METHOD FOR CHARACTERIZING AND MULTI-DIMENSIONALLY REPRESENTING THE FOLDING PROCESS OF PROTEINS - The invention relates to a novel method for characterizing and multi-dimensionally representing the folding process of proteins (FIG. | 2013-05-23 |
20130130295 | CARBON NANOTUBE BASED IMAGING AGENTS - Compositions and methods related to carbon nanotubes are provided. More particularly, imaging agents comprising carbon nanotubes internally loaded with a contrast agent and associated methods are provided. One example of a method may involve a method for imaging comprising: providing an imaging agent comprising a carbon nanotube loaded with contrast agent; introducing the imaging agent into a cell; and imaging the cell to detect the presence of the imaging agent. | 2013-05-23 |
20130130296 | Modular Functional Peptides for Delivery of Nanoparticles - A peptide directs nanoparticles (such as quantum dots) to the plasma membrane of mammalian cells. A method of delivery of a nanoparticle to a plasma membrane of a cell includes providing to the cell a nanoparticle attached to a peptide configured to direct the nanoparticle the plasma membrane, and allowing the cell to take up the nanoparticle. The nanoparticle can be a FRET donor to an organic dye. | 2013-05-23 |
20130130297 | INDUCTION OF A MATURE HEPATOCYTE PHENOTYPE - The present invention relates to a method for producing cells having a mature hepatocyte phenotype, comprising (a) providing a cell population comprising precursor cells of mature hepatocytes, wherein said cell population is obtained from a subject or derived from a cell line; and (b) introducing into said precursor cells a group of differentiation factors and/or the nucleic acid sequences encoding said differentiation factors, said group consisting of (i) one or more member(s) of the Foxa subfamily, (ii) HNF-4α and (iii) C/EBPα, thereby differentiating said precursor cells into cells having a mature hepatocyte phenotype. | 2013-05-23 |
20130130298 | BLOOD PRODUCT MANAGEMENT METHOD USING RBC DEFORMABILITY-BASED METRICS - A method for using red blood cell deformability testing to improve management of blood product comprising RBC, the method comprising: generating deformability data for red blood cells corresponding to a respective unit of blood product; correlating the deformability data with red blood cell viability or efficacy based on any available direct or indirect in vivo performance data; obtaining a representation of quality for the respective unit of blood product; and based on the representation of quality assigning a relative rank to and/or timing a transfer of the respective unit in an inventory of stored blood product units. | 2013-05-23 |
20130130299 | ELECTROCHEMICAL FLOW CYTOMETRY - A method for triggering cellular exocytosis events and measuring quantal release of electroactive chemicals is disclosed. A method can include flowing a cell through a single cell channel having a pair of electrodes oriented to direct current through the cell at a stimulation location along the single cell channel. The pair of electrodes include a working electrode, a counter electrode and an optional reference electrode. The single cell channel has dimensions which provide direct contact of the cell with walls of the single cell channel sufficient to substantially reduce or eliminate shunt paths around the cell at the stimulation location. The cell can be exposed to an electric field at the stimulation location using the pair of electrodes sufficient to trigger exocytosis. Changes in current due to exocytosis processes appear as spikes that can be correlated with a quanta of the electroactive chemicals which are released during exocytosis. | 2013-05-23 |
20130130300 | METHOD AND APPARATUS FOR THE NON-INVASIVE MONITORING OF GAS EXCHANGE BY BIOLOGICAL MATERIAL - The invention provides an indirect pressure sensing system for non-invasive measurement of primary pressure in a sealed container, which communicates primary pressure changes from within the container, via a flexible diaphragm, to a secondary chamber wherein there is a defined relationship between the primary and secondary pressures, which enables a pressure sensor in the secondary chamber to generate a signal representing primary pressure in the sealed container, but to remain isolated from the contents of the sealed container. The pressure sensor can provide electrical outputs representing the pressure detected, and the outputs are fed to data processing means capable of producing a measurement of primary pressure. The system can have a liquid culture of cellular material (eg. micro organisms, plant tissue cells, animal cells etc) partially filling the container, whereby the metabolism and/or growth of cellular material causes gas exchanges between liquid and headspace, which can result in primary pressure changes. | 2013-05-23 |
20130130301 | MICROFLUIDIC PLATFORM FOR DISCRETE CELL ASSAY - A microfluidic chamber for use in individual cell assays. The microfluidic chamber includes a cell microchamber having an interior region and front and rear valves, each of which are separately controllable so that they can be selectively opened and closed to thereby permit the transference of an individual cell into and out of the interior region. Cell secretion and contact interaction studies can be carried out using the microchambers, with the valves permitting either complete isolation or perfusion media flow through the microchambers. An internal perfusion wall can be included to partition the microchamber for non-contact perfusion studies of secretion interactions between cells. | 2013-05-23 |
20130130302 | POST PROTEIN HYDROLYSIS REMOVAL OF A POTENT RIBONUCLEASE INHIBITOR AND THE ENZYMATIC CAPTURE OF DNA - The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample. | 2013-05-23 |
20130130303 | METHODS FOR SCREENING MICROBIAL REMEDIATION AGENTS - Disclosed are methods for determining the efficacy of antimicrobial agents used in the treatment of building materials after microbial contamination has occurred, for the purpose of killing existing microbial growth and reducing or inhibiting recurrent or subsequent microbial growth. The disclosed methods may be used to determine microbial growth at time points subsequent to antimicrobial treatment of the material surface. The disclosed invention also measures visible microbial growth in a semi-quantitatively analysis. In addition, the Inventors disclosure a method with reduced variability and a more accurate assessment of antimicrobial efficacy. | 2013-05-23 |
20130130304 | METHODS FOR SCREENING MICROBIAL GROWTH INHIBITION ACTIVITY ON MATERIALS - Disclosed are methods of determining the effectiveness of an antimicrobial agent in reducing or inhibiting microbial growth on a substrate. The disclosed methods may be used to determine microbial growth at time points subsequent to antimicrobial treatment of the material surface and exposure to microorganisms. The disclosed invention also measures visible microbial growth using a semi-quantitatively method that is more accurate and less subjective than estimates of growth used previously. The disclosed method that provides an accurate assessment of antimicrobial efficacy with reduced variability. | 2013-05-23 |
20130130305 | INDENTIFYING ANTIFUNGAL AGENTS THAT INHIBIT IAA OR A YAP FAMILY - The present invention relates to methods of screening for antifungal agents by identifying agents that bind to or otherwise inhibit indole-3-acetic acid (IAA) or that bind to or otherwise inhibit the expression or activity of a protein within the Yap family or a gene encoding a protein within the Yap family. | 2013-05-23 |
20130130306 | ZN (II) BASED COLORIMETRIC SENSOR AND PROCESS FOR THE PREPARATION THEREOF - A colorimetric chemosensor molecule having a aza-macrocycle Zn (II)-complex (L.Zn) (Scheme 1, Formula 1A) which can recognize selectively and efficiently ATP (Adenosine triphosphate), a biologically significant triphosphate in aqueous medium at pH 7.4 is described. Since ATP is the source of energy in living organisms, L.Zn (Scheme 1, Formula 1A) can also be used as a staining agent in the living cells through binding to ATP, generated in situ during the metabolic process. | 2013-05-23 |
20130130307 | CELL OBSERVATION DEVICE AND CELL OBSERVATION METHOD - A cell observation device is provided with a reflection interference shutter | 2013-05-23 |
20130130308 | PROCESS FOR DIRECTLY MEASURING MULTIPLE BIODEGRADABILITIES - A method for measuring the biodegradability of organic substrates by the fluorescent and/or colorimetric detection of the microbial activity generated by the addition of organic substrates to a mixture of microorganisms. | 2013-05-23 |
20130130309 | Radiopharmaceutical Production System and Quality Control System Utilizing High Performance Liquid Chromatography - HPLC-based quality control systems to perform quality control testing on a radiopharmaceutical solution shortly after synthesis. An HPLC-based quality control system makes efficient use of sample volume and is compatible with a variety of radioisotopes and radiopharmaceutical compounds. In several embodiments, the automated nature of an HPLC-based quality control system allows for quality control tests to be conducted quickly and with minimal impact on user workflow. When used as part of an integrated PET biomarker radiopharmaceutical production system, the present general inventive concept permits a manufacturer to produce product and conduct quality control tests with lower per dose costs. | 2013-05-23 |
20130130310 | ABSORBENT PAPER AND USE THEREOF FOR BREAST CANCER DETECTION - Biological samples of mammary fluid or components thereof are obtained using a breast pump device coupled with an absorbent paper or membrane, optionally facilitated by administering oxytocin to the subject. The breast pump device stimulates expression of mammary fluid and provides for collection of diagnostic samples on the absorbent paper or membrane to evaluate breast disease, including cancer. The biological sample may include fluid containing one or more of cells or cellular components, proteins, glycoproteins, peptides, nucleotides or other desired constituents comprising a breast disease marker. Absorbent paper or membrane, and methods relating to the paper or membrane, and a breast pump device are also provided. | 2013-05-23 |
20130130311 | METHODS AND SYSTEMS FOR ASSESSING CLONALITY OF CELL CULTURES - Methods of determining clonality of a cell culture are provided. Also provided are systems employing the above methods in high throughput sample screening. | 2013-05-23 |
20130130312 | METHODS AND STRAINS FOR THE PRODUCTION OF SARCINAXANTHIN AND DERIVATIVES THEREOF - The present invention relates to a new strain of | 2013-05-23 |
20130130313 | METHOD OF SYNTHESIZING A SUPPRESSOR tRNA, DNA CONSTRUCT AND USE THEREOF FOR PRODUCING A NON-NATURAL AMINO ACID-INCORPORATED PROTEIN - There are provided a DNA construct comprising a suppressor tRNA gene of a non-eukaryote containing no internal promoter functioning in a eukaryotic cell, and a eukaryotic or bacteriophage promoter linked at the 5′ end of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing protein incorporating a non-natural amino acid by using the same. | 2013-05-23 |
20130130314 | Expression of Proteins in Plants - The invention relates to a method of producing an influenza virus H5 polypeptide in a plant comprising the steps of cloning an influenza H5 gene or nucleic acid encoding its functional equivalent into a vector adapted to target components present in the plant, infiltrating at least a portion of the plant with the vector or transforming plant tissue with the vector so as to transiently express the influenza virus H5 polypeptide, and/or to create a transgenic plant; and recover the influenza virus H5 polypeptide expressed by the plant. The invention further relates to vectors, transgenic plants or parts thereof and the progeny of such plants used in or which come about as a result of the method. | 2013-05-23 |
20130130315 | FUSION PROTEIN - An isolated nucleic acid molecule selected from the group consisting of: vi. a nucleic acid molecule comprising a nucleotide sequence which is at least 85% identical to the nucleotide sequence of SEQ ID NO:1 or a complement thereof; vii. a nucleic acid molecule comprising a fragment of at least 1500 consecutive nucleotides of the nucleotide sequence of SEQ ID NO:1, or a complement thereof; viii. a nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence at least 85% identical to SEQ ID NO:2; ix. a nucleic acid molecule which encodes a fragment of a polypeptide comprising the amino acid sequence of SEQ ID NO:2, wherein the fragment comprises at least 500 contiguous amino acids of SEQ ID NO: 2; and x. a nucleic acid molecule encoding a polypeptide containing a humanized immunoglobulin or parts of an immunoglobulin having binding specificity for CD133 a nucleic acid molecule which encodes a variant of a polypeptide comprising the amino acid sequence of SEQ ID NO: 2, wherein the nucleic acid molecule hybridizes to a nucleic acid molecule comprising the entire SEQ ID NO: 1, or complement thereof under conditions of incubation at 45° C. in 6.0×SSC followed by washing in 0.2×SSC/0.1% SDS at 65° C. | 2013-05-23 |
20130130316 | CELL CULTIVATION PROCESS - This invention relates to a cell culture process for the production of polypeptides in mammalian CHO cells characterized by one or more temperature and pH shifts which are adjusted in respect to their timing and step size to reduce cell death, increase product yield and improve product quality. | 2013-05-23 |
20130130317 | METHOD FOR PRODUCING SUBSTANCE - The present invention provides a medium suitable for animal cell culture, a culture method using the same, and the like. The present invention relates to a method for culturing animal cells having an ability to produce a substance, which comprises culturing the animal cells in a medium supplemented with an oligopeptide having one or more L-cysteines and excluding glutathione, a method for producing a substance by culturing animal cells having the ability to produce the substance, which comprises culturing the animal cells in a medium supplemented with an oligopeptide having one or more L-cysteines and excluding glutathione to produce and accumulate the substance in the culture, and collecting the substance from the culture, and a culture medium comprising an oligopeptide having one or more L-cysteines and excluding glutathione. | 2013-05-23 |
20130130318 | PROCESS FOR OBTAINING BIOCHEMICALS IN A ZERO-LIQUID DISCHARGE PLANT - A method is presented for the production of cellulosic ethanol, acetic acid and derivatives from the extract containing fibers and hemicelluloses after steam cooking of biomass in a host plant. The process is integrated with the host plant process to minimize the effect of loss of heat value from the extracted hemicelluloses and eliminate the need for the waste water treatment plant. | 2013-05-23 |
20130130319 | CELLS AND METHODS FOR PRODUCING RHAMNOLIPIDS - The invention relates to cells and nucleic acids and also use thereof for producing rhamnolipids, and also methods for producing rhamnolipids. | 2013-05-23 |
20130130320 | ENZYMES - The invention relates to a nucleic acid polymerase capable of producing a non-DNA nucleotide polymer from a DNA nucleotide polymer template, said polymerase comprising amino acid sequence having at least 36% identity to the amino acid sequence of SEQ ID NO:1, wherein said amino acid sequence is mutated relative to the amino acid sequence of SEQ ID NO:1 at one or more residues of the thumb region, said residues selected from: amino acids 651 to 679 (patch 10A); wherein said amino acid sequence is mutated relative to the amino acid sequence of SEQ ID NO:1 at residue E664. In one embodiment said polymerase comprises the mutations Y409G and E664K. In one embodiment said polymerase comprises amino acid sequence corresponding to SEQ ID NO:12. The invention also relates to A nucleic acid polymerase capable of reverse transcribing a HNA nucleotide polymer into a DNA nucleotide polymer, said polymerase comprising amino acid sequence having at least 36% identity to the amino acid sequence of SEQ ID NO:1, wherein said amino acid sequence is mutated relative to the amino acid sequence of SEQ ID NO:1 at residue I521. | 2013-05-23 |
20130130321 | Programmable Oligonucleotide Synthesis - The invention relates to methods and devices for preparing synthetic nucleic acids. | 2013-05-23 |
20130130322 | Modified RNA Ligase for Efficient 3` Modification of RNA - The invention provides a novel truncated mutated T4 RNA ligase 2. In addition, methods are provided for ligating pre-adenlylated donor molecules to the 3′ hydroxyl group of RNA in the absence of ATP using the ligase. | 2013-05-23 |
20130130323 | PREVENTION AND ALLEVIATION OF STERIC HINDRANCE DURING SINGLE MOLECULE SYNTHESIS - The present invention provides compositions and methods for reducing steric hindrance in the product of nucleic acid polymerase reaction. Methods and compositions of the invention encompass application of exonucleases, endonucleases, and uracil-DNA glycosylases to a nucleic acid polymerase reaction such that newly formed nucleic acid strands are modified (e.g., cleaved) while the polymerase reaction continues to proceed. | 2013-05-23 |
20130130324 | GENETICALLY ENGINEERED STRAIN WSJ-IA FOR PRODUCING ISOVALERYL SPIRAMYCIN I - A genetically engineered strain WSJ-IA for producing isovaleryl spiramycin I. Also provided is a method for preparing the strain, comprising the steps of: (a) constructing a recombinant plasmid comprising a double gene ist-acyB2; (b) transforming the plasmid into an isovaleryl spiramycin I—producing strain to obtain the strain WSJ-IA. The level of isovaleryl spiramycin I produced by fermentation of the strain WSJ-IA is increased 1.7 times and the fermentation potency thereof increased 4.14 times in comparison with the strain exclusively comprising a single gene ist. | 2013-05-23 |
20130130325 | Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase and Beta-Xylosidase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2013-05-23 |
20130130326 | Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase and Beta-Xylosidase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2013-05-23 |
20130130327 | Polypeptides Having Beta-Glucosidase Activity, Beta-Xylosidase Activity, or Beta-Glucosidase and Beta-Xylosidase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2013-05-23 |
20130130328 | METHOD OF TREATING PLANT BIOMASS - Plant biomass is immersed in a solution that contains a polar solvent and an imidazolium salt that has a melting point of at least 100° C. As a result, the cellulose and hemicellulose present in the plant biomass are relaxed (decrystallized and depolymerized) and brought into an easy-to-degrade state. Reacting the immersed plant biomass with a cellulase produces saccharide at a high conversion efficiency. | 2013-05-23 |
20130130329 | METHOD FOR EXTRACTING SUBSTANCES FROM SOAPBERRY FRUIT AND ITS SEEDS - An exclusive method for extracting active interface saponin and organic substances from soapberry; organic elements and oleic alcohol products from soapberry seeds through fermenting process, and end products made therefrom. By this method, every part of the soapberry is processed to become the raw material of varies of products and daily necessaries. Said method is toxin free and biologically safe, produce no solid and liquid wastage, zero carbon emissions, zero chemical pollution, low energy consumption and ecologically friendly. The end products produced by present invention are variables which can be applied in cosmetics, medicals, cleaning products, skin caring products and so on, thus, are with excellent economic value and industrial viability in mass production. | 2013-05-23 |
20130130330 | NOVEL GLYCOSYL HYDROLASE WITH BETA-XYLOSIDASE AND BETA-GLUCOSIDASE ACTIVITIES AND USES THEREOF - A novel glycosyl hydrolase with activities of beta-xylosidase and beta-glucosidase is provided. Said glycosyl hydrolase can convert 7-xylosyltaxane compounds to 7-hydroxyltaxane compounds. | 2013-05-23 |
20130130331 | METHOD OF PRODUCING SUGARS USING A COMBINATION OF ACIDS TO SELECTIVELY HYDROLYZE HEMICELLULOSIC AND CELLULOSIC MATERIALS - A method is provided for producing sugars using a combination of acids to hydrolyze hemicellulosic and cellulosic materials in biomass, said combination of acids namely comprising a first, weak organic acid (such as acetic acid or formic acid) for providing a pentose product or stream from hydrolyzing hemicellulosic materials in the biomass on a batchwise, semi-continuous or continuous basis, and a second, strong mineral acid (such as sulfuric acid) for providing a hexose product or stream from hydrolyzing cellulosic materials in the biomass. | 2013-05-23 |
20130130332 | NOVEL METHOD FOR PREPARING PTEROCARPAN - The present invention relates to a novel method for preparing pterocarpan from isoflavan-4-ol. | 2013-05-23 |
20130130333 | Processes for Making (R)-Ethyl 4-Cyano-3 Hydroxybutyric Acid - The invention provides novel processes for making ethyl-4-cyano-3-hydroxybutyrate, e.g., (R)-ethyl 4-cyano-3-hydroxybutyric acid, and 4-cyano-3-hydroxybutyric acid. The invention provides protocols for making and 4-cyano-3-hydroxybutyric acid and ethyl-4-cyano-3-hydroxybutyrate by whole cell processes, cell lysate processes, “one pot processes” and “multi-pot” processes using a variety of parameters. | 2013-05-23 |
20130130334 | BIOFUEL AND ELECTRICITY PRODUCING FUEL CELLS AND SYSTEMS AND METHODS RELATED TO SAME - A fuel cell comprising an anode electrode, a cathode electrode and a reference electrode electronically connected to each other; a first biocatalyst comprising a consolidated bioprocessing organism (e.g., a cellulomonad or | 2013-05-23 |
20130130335 | Purification Methods and Systems Related to Renewable Materials and Biofuels Production - Methods of producing renewable materials may include consuming a fermentation feedstock with a fermentation organism to produce a renewable material in fermentation broth; water may then be separated from the feedstock or broth using one or more phase separations, or the renewable material may be concentrated from the feedstock or broth using one or more phase separations. Methods of producing biofuel components may include consuming a lignocellulosic or sugar fermentation feedstock with a fermentation organism to produce either ethanol or butanol in fermentation broth; cooling the feedstock or broth to solidify at least some water therein; and separating the solidified water from the feedstock or broth using a solid-liquid phase separation. | 2013-05-23 |
20130130336 | CHAIN-SELECTIVE SYNTHESIS OF FUEL COMPONENTS AND CHEMICAL FEEDSTOCKS - A method comprising providing a starting composition comprising a polyunsaturated fatty acid, a polyunsaturated fatty ester, a carboxylate salt of a polyunsaturated fatty acid, a polyunsaturated triglyceride, or a mixture thereof; self-metathesizing the starting composition or cross-metathesizing the starting composition with at least one short-chain olefin in the presence of a metathesis catalyst to form self-/cross-metathesis products comprising: cyclohexadiene; at least one olefin; and one or more acid-, ester-, or salt-functionalized alkene; and reacting cyclohexadiene to produce at least one cycloalkane or cycloalkane derivatives. A method for producing cycloalkanes for jet fuel by providing a starting composition comprising at least one selected from the group consisting of algal and polyunsaturated vegetable oils, subjecting the starting composition to metathesis to produce metathesis product comprising at least one olefin, cyclohexadiene, and at least one acid-, ester-, or salt-functionalized alkene, and reacting the at least one olefin and cyclohexadiene to form cycloalkane(s). | 2013-05-23 |
20130130337 | Enzymatic Acylation Method Using an Acylphosphonate Donor - The invention relates to an enzymatic acylation method including at least the following steps of: contacting at least one compound having at least one function selected from among the amine, alcohol, or thiol functions, at least one microorganism having an acyl transfer activity and/or an acyl transfer enzyme, and at least one acylphosphonate donor of formula (I), where: R is an alkyl, alkene, uikyne, aryl, or aralkyl radical, or is —ORa, —SRa, —NRaRb, where Ra and Rb are identical or different and are H, an alkyl, alkene, alkyne, aryl or aralkyl radical, the alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; X is O or S; Y and Z, which are identical or different, are —OR1, —OR2, —SR1, —SR2, —NR′1R″1, —NR′2R″2; R1, R2, R′1, R′2, R″1 and R″2, which are identical or different, are an alkyl, alkene, alkyne, aryl or aralkyl radical, said alkyl, alkene, alkyne, aryl or aralkyl radicals being optionally substituted; and recovering the compound including at least one acyl function, said function being selected from among the amine, alcohol, or thiol functions. | 2013-05-23 |
20130130338 | NOVEL HYDROLASE PROTEIN - It is an object of the present invention to provide a novel hydrolase, which is used when dialkyl 2-vinylcyclopropane-1,1-dicarboxylate is hydrolyzed with an enzyme, so as to efficiently obtain (1S,2S)-1-alkoxycarbonyl-2-vinylcyclopropanecarboxylic acid that is useful as an intermediate for synthesizing therapeutic agents for hepatitis C. According to the present invention, there is provided a hydrolase protein, which consists of the amino acid sequence shown in any one of SEQ ID NOS. 2 to 5 and which has activity of catalyzing, at higher selectivity than the protein consisting of the amino acid sequence shown in SEQ ID NO. 1, a reaction of producing (1S,2S)-1-ethoxycarbonyl-2-vinylcyclopropanecarboxylic acid from diethyl 2-vinylcyclopropane-1,1-dicarboxylate. | 2013-05-23 |
20130130339 | FERMENTATION PROCESS FOR THE PRODUCTION OF ORGANIC ACIDS - This invention relates to improvements in the fermentation process used in the production of organic acids from biological feedstock using bacterial catalysts. The improvements in the fermentation process involve providing a fermentation medium comprising an appropriate form of inorganic carbon, an appropriate amount of aeration and a biocatalyst with an enhanced ability to uptake and assimilate the inorganic carbon into the organic acids. This invention also provides, as a part of an integrated fermentation facility, a novel process for producing a solid source of inorganic carbon by sequestering carbon released from the fermentation in an alkali solution. | 2013-05-23 |
20130130340 | BIOSYNTHESIS OF CAFFEIC ACID AND CAFFEIC ACID DERIVATIVES BY RECOMBINANT MICROORGANISMS - Microorganisms are genetically engineered to synthesize caffeic acid from simple carbon sources via a tyrosine intermediate by means of a dual pathway that utilizes both endogenous and engineered enzymatic activities. | 2013-05-23 |
20130130341 | ELECTRO-AUTOTROPHIC SYNTHESIS OF HIGHER ALCOHOLS - The disclosure provides a process that converts CO | 2013-05-23 |
20130130342 | Yeast Strain for Production of Four Carbon Alcohols - Yeast cells with a reduced general control response to amino acid starvation were found to have increased tolerance to butanol in the growth medium. The reduced response was engineered by genetic modification of a gene involved in the response, a GCN gene, to eliminate activity of the encoded protein. Yeast strains with an engineered butanol biosynthetic pathway and a genetic modification in a gene involved in the general control response to amino acid starvation, which have increased butanol tolerance, are useful for production of butanol. | 2013-05-23 |
20130130343 | PROCESSES AND SYSTEMS FOR DRY-MILLED CORN ETHANOL AND CORN OIL PRODUCTION WITH IMPROVED CARBON FOOTPRINT - The present invention improves corn dry milling in several ways. Integrated corn biorefinery processes are disclosed which can produce ethanol, edible corn oil, DDGS, solvent-extracted meal, power, and optionally crude corn oil, starting from corn. Some variations employ corn fractionation and edible corn oil recovery using liquid carbon dioxide, avoiding hazardous hydrocarbon-based solvents to produce edible corn oil. Some variations employ integration of gas-fired co-generation into the dry-milled corn ethanol plant to significantly reduce energy usage and carbon footprint associated with the overall process. Counter-current drying is preferably employed to produce a high-quality DDGS product with high protein content, low mycotoxin content, and low residual ethanol content. | 2013-05-23 |
20130130344 | MICROORGANISM VARIANTS HAVING HYDROCARBON PRODUCING ABILITY AND METHOD FOR PRODUCING HYDROCARBON USING THE SAME - The present invention relates to a microorganism variant having the ability to produce hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same, and more particularly, to a microorganism variant obtained by introducing a gene encoding an enzyme converting fatty acyl-acp to free fatty acid, a gene encoding an enzyme converting free fatty acid to fatty acyl-CoA, a gene encoding an enzyme converting fatty acyl-CoA to fatty aldehyde and a gene encoding an enzyme converting fatty aldehyde to alkane into a microorganism improved so as to be suitable for the production of hydrocarbons, including alkane, and a method of producing hydrocarbons, including alkane, using the same. The microorganism variant of the present invention has high potential to be used to improve strains by additional metabolic flux engineering, and thus is useful for the industrial production of hydrocarbons, including alkane. | 2013-05-23 |
20130130345 | PRODUCTION OF RENEWABLE AROMATIC COMPOUNDS - The invention provides a process for producing a variety renewable aromatic compounds such as benzene, toluene, xylenes, and cumene, as well as compounds derived from these including, for example, aniline, benzoic acid, cresol, cyclohexane, cyclohexanone, phenol and bisphenol A, toluene di-isocyanate, isophthalic acid, phthalic anhydride, terephthalic acid and dimethyl terephthalate. The invention also provides for renewable forms of these aromatic compounds. | 2013-05-23 |
20130130346 | Human Waste Treatment System and Method - A human waste treatment system is disclosed that includes at least one waste receptacle such as an airline-style toilet, an anaerobic digester such as an induced bed reactor, and a gas conditioner. Human waste may be moved by a macerator pump. Inside the digester, bacteria digests organic solids to form biogas. The anaerobic digester may be operated at thermophilic temperatures to kill pathogenic bacteria in the waste and produce treated water. The gas conditioner purifies the biogas which may be used to power an electric generator. Treated water may be used to flush the system. The system may be mounted on a semi-truck trailer and transported. The system may be self-contained. | 2013-05-23 |
20130130347 | CONSTRUCTS AND METHODS FOR THE ASSEMBLY OF BIOLOGICAL PATHWAYS - The present invention is directed to a synthetic nucleic acid scaffold comprising one or more subunits, each subunit comprising two or more different protein-binding sequences coupled together. The present invention further relates to systems and methods for assembling a synthetic biological pathway and producing a biological pathway product or a precursor product using the synthetic nucleic acid scaffold. | 2013-05-23 |
20130130348 | Polymers for Functional Particles - A method includes producing libraries of nanoparticles having highly controlled properties, which can be formed by mixing together two or more macromolecules in different ratios. One or more of the macromolecules may be a polymeric conjugate of a moiety to a biocompatible polymer. The nanoparticle may contain a drug. The moiety may include a polypeptide or a polynucleotide, such as an aptamer. The moiety may be a targeting moiety, an imaging moiety, a chelating moiety, a charged moiety, or a therapeutic moiety. Another aspect is directed to systems and methods of producing such polymeric conjugates. In some embodiments, a solution containing a polymer is contacted with a liquid, such as an immiscible liquid, to form nanoparticles containing the polymeric conjugate. Other methods use such libraries, use or administer such polymeric conjugates, or promote the use of such polymeric conjugates. Kits involving such polymeric conjugates are also described. | 2013-05-23 |
20130130349 | COOPERATIVE AND DYNAMIC ASSEMBLY OF AFFINITY COMPLEXES - The invention generally relates to the field of immunochemistry including antibody therapy, diagnostics, and basic research and specifically relates to the area of alternatives to natural antibodies including artificial antibodies or antibody mimics. The invention relates particularly to the cooperative assembly of stable affinity complexes. | 2013-05-23 |
20130130350 | OBLIGATE HETERODIMER VARIANTS OF FOKI CLEAVAGE DOMAIN - Disclosed are methods of making and using engineered FokI cleavage domain variants. Also disclosed are methods, compositions and fusion proteins containing obligate heterodimers of engineered FokI cleavage domain variants and DNA binding domains, such as zinc finger protein (ZFP) domains and transcription activator-like effector (TALE) domains. | 2013-05-23 |
20130130351 | VARIANT LOVD POLYPEPTIDES AND THEIR USES - The present disclosure provides acyltransferases useful for synthesizing therapeutically important statin compound | 2013-05-23 |
20130130352 | CONTAMINATION-FREE REAGENTS FOR NUCLEIC ACID AMPLIFICATION - Methods and kits for generating contamination-free reagents and reagent solutions for use in nucleic acid amplification are provided. Methods include processing of polymerase solutions, nucleotide solutions and primer solutions to render contaminating nucleic acid inert. The methods employ the proofreading activity of the polymerase and/or exonucleases to de-contaminate the reagents and reagent solutions. Methods and kits for contamination-free nucleic acid amplification are provided. | 2013-05-23 |
20130130353 | Variants of Glycoside Hydrolases - The present invention relates to variants of a parent glycoside hydrolase, comprising a substitution at one or more positions corresponding to positions 21, 94, 157, 205, 206, 247, 337, 350, 373, 383, 438, 455, 467, and 486 of amino acids 1 to 513 of SEQ ID NO: 2, and optionally further comprising a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2 a substitution at one or more positions corresponding to positions 8, 22, 41, 49, 57, 113, 193, 196, 226, 227, 246, 251, 255, 259, 301, 356, 371, 411, and 462 of amino acids 1 to 513 of SEQ ID NO: 2, wherein the variants have glycoside hydrolase activity. The present invention also relates to nucleotide sequences encoding the variant glycoside hydrolases and to nucleic acid constructs, vectors, and host cells comprising the nucleotide sequences. | 2013-05-23 |
20130130354 | Novel genes and uses thereof, expression profile of colon, gastric and pancreatic cancer - This invention provides information on differentially expressed genes in malignant tissue of gastric, colon and pancreatic adenocarcinomas as compared to their corresponding adjacent non-malignant tissues. These genes or their products can be used as targets in developing new strategies for the treatment and diagnosis of these gastrointestinal cancers. | 2013-05-23 |
20130130355 | METHOD AND SYSTEM OF PARTICLE-PHAGE EPITOPE COMPLEX - The present disclosure provides compositions and methods for using phage epitopes to profile the immune response. The phage epitopes can be used to detect one or more antibodies from a sample. Furthermore, the present disclosure provides methods and compositions for detecting a cancer based on the detection of one or more antibodies. In one embodiment, the antibody is an autoantibody. The present disclosure also provides methods of producing antibody detecting complexes. | 2013-05-23 |
20130130356 | Serum-Free Growth Medium For Acholeplasma Laidlawii And Methods For Retention Testing Sterilizing Grade Filters - A method for retention testing sterilizing grade filters comprises: a) providing a stock of | 2013-05-23 |
20130130357 | Anaerobic Digestion Method - This anaerobic digestion method is a method to biologically treat precipitated sludge obtained by a precipitating operation to sewage or wastewater, sludge containing livestock waste sludge, or organic wastewater containing suspended solids of 5000 mg/L or more as a treatment object, by a fixed bed ( | 2013-05-23 |
20130130358 | Machine Dishwashing Compositions and Methods - A machine dishwashing main-wash detergent composition or a dishwashing machine cleaning composition comprising at least one cellulase enzyme, and at least one pectinase enzyme is provided. The compositions optionally also comprise at least one lipase, surfactant (especially non-ionic surfactant) and a pH buffering system. | 2013-05-23 |
20130130359 | SYSTEM FOR COLLECTING AND PRESERVING TISSUE CORES - A cooling system for preserving tissue is disclosed. The cooling system comprises a base member, a temperature control sleeve constructed of a thermally conductive material, and a selectively removable lid member. The base member defines a reservoir and receives the temperature control sleeve. The temperature control sleeve at least partially defines a tissue collector chamber that is configured to receive a tissue collector. The temperature control sleeve is in communication with the reservoir. The reservoir is configured to receive a cooling medium. A slit formed within the tissue collection chamber that is sized to receive a tubing connected to the tissue collector therethrough. The lid member is configured to be selectively attached to the base member, and permit access to a tube mount for the tissue collector when the lid is attached to the base member. | 2013-05-23 |
20130130360 | APPARATUS AND METHODS FOR MANIPULATION AND OPTIMIZATION OF BIOLOGICAL SYSTEMS - The invention provides systems and methods for manipulating biological systems, for example to elicit a more desired biological response from a biological sample, such as a tissue, organ, and/or a cell. In one aspect, the invention operates by efficiently searching through a large parametric space of stimuli and system parameters to manipulate, control, and optimize the response of biological samples sustained in the system In one aspect, the systems and methods of the invention use at least one optimization algorithm to modify the actuator's control inputs for stimulation, responsive to the sensor's output of response signals. The invention can be used, e.g., to optimize any biological system, e.g., bioreactors for proteins, and the like, small molecules, polysaccharides, lipids, and the like. Another use of the apparatus and methods includes is for the discovery of key parameters in complex biological systems. | 2013-05-23 |
20130130361 | PROCESSING SYSTEM FOR CELL CULTURES AND MODULE CONNECTING METHOD OF PROCESSING SYSTEM FOR CELL CULTURES - A processing system for cell cultures for carrying out cell/tissue culturing processes in the field of regenerative medicine, etc. which is a processing system for cell cultures which prevents viruses and human-derived cells, etc. other than a culturing processing target from entering the interior of a closed space from outside of the system to maintain sterility, maintains sealability of the closed spaces of modules, and couples or detaches the plurality of modules in accordance with a wide variety of cell culturing processing steps so that cell culturing processes can be carried out by combining the plurality of culturing processing modules is provided. | 2013-05-23 |
20130130362 | POWER SPECTRAL DENSITY CHEMICAL AND BIOLOGICAL SENSOR - A surface acoustic wave (SAW) based sensor device and system for detecting the presence of and measuring the concentration of chemical and biological analytes in vapor and liquid phase can include inherent temperature compensation and the capability to operate in a wired mode or in a wireless mode with the ability to measure the distance of the sensor from the wireless transceiver (in addition to measuring temperature and the chemical and/or biological analytes of interest). This device can also monitor changes in state of thin films, including but not limited to sensing glassy to rubbery transitions in polymers, and measurement of the kinetics of chemical and/or biological processes occurring at the surface of the device. Coding, time, and frequency diversity can be included in the device structure to enable production of groups of individually identifiable sensor devices capable of operating simultaneously within the field of view of a wireless transceiver. | 2013-05-23 |
20130130363 | SUBSTRATES, SYSTEMS AND METHODS FOR ANALYZING MATERIALS - Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses. | 2013-05-23 |
20130130364 | MICRODEVICE FOR PATHOGEN DETECTION - There is provided a microdevice for biomaterial detection, including a passive micromixer to mix a biomaterial, a first probe, and a second probe; a magnetic separation chamber connected with the passive micromixer; and a capillary electrophoresis channel connected with the magnetic separation chamber. | 2013-05-23 |
20130130365 | DEVICES - A method of analysis, instrument for analysis and device for use in such an instrument are provided, which perform a number of processes need to reach a useful result in the context of a wide variety of samples. The sequence of those processes being optimised. A device, instrument using the device and method of use are also provided which offer reliable performance of a heating based process, with minimal condensation and/or sample loss issues. | 2013-05-23 |
20130130366 | IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD - An automated in situ heat induced antigen recovery and staining method and apparatus for treating a plurality of microscope slides. The process of heat induced antigen recovery and the process of staining the biological sample on the microscope slide are conducted in the same apparatus, wherein the microscope slides do not need to be physically removed from one apparatus to another. Each treatment step occurs within the same reaction compartment. The reaction conditions of each reaction compartment for treating a slide can preferably be controlled independently, including the individualized application of reagents to each slide and the individualized treatment of each slide. | 2013-05-23 |
20130130367 | Method And Device For Combined Detection Of Viral And Bacterial Infections - A lateral flow assay detects and differentiates between viral and bacterial infections. A combined point of care diagnostic device tests markers for viral infection and markers for bacterial infection, to effectively assist in the rapid differentiation of viral and bacterial infections. In one preferred embodiment, the bacterial marker is CRP. In another preferred embodiment, the viral marker is MxA. In some embodiments, it is unnecessary to lyse the cells in the sample prior to applying it to the device. | 2013-05-23 |
20130130368 | INTEGRATED MICROBIAL COLLECTOR - A system for real-time sizing of fluid-borne particles is disclosed. The system further determines, in real time, whether the detected particles are biological or non-biological. As the fluid is being tested, it is exposed to a microbe collection filter which is cultured to determine the type of microbes present in the fluid being tested. | 2013-05-23 |
20130130369 | SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS - Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. | 2013-05-23 |
20130130370 | MicroRNA and Methods for Inhibiting Same - The invention relates to isolated DNA or RNA molecules comprising at least ten contiguous bases having a sequence in a pancreatic islet microRNA. In another embodiment, the invention relates to isolated single stranded pancreatic islet microRNA molecules or anti-pancreatic islet microRNA molecules. | 2013-05-23 |
20130130371 | Lipoaspirate stem cell separation system and methods thereof - A system and method for isolating and separating lipoaspirate particles. The system includes a generally cylindrical container having a lid and a bottom wherein the container includes at least one input port positioned to permit a lipoaspirate fluid to enter the container above the bottom; and a source of a vacuum coupled to the container to provide a partial vacuum during use of the system. | 2013-05-23 |
20130130372 | ENHANCED EXPRESSION AND STABILITY REGIONS - Expression-enhancing nucleotide sequences for expression in eukaryotic systems are provided that allow for enhanced and stable expression of recombinant proteins in eukaryotic cells. Enhanced expression and stability regions (EESYRs) are provided for expression of a gene of interest in a eukaryotic cell. Chromosomal loci, sequences, and vectors are provided for enhanced and stable expression of genes in eukaryotic cells. | 2013-05-23 |
20130130373 | Kit Comprising Serum Replacement and Labile Factors - The present disclosure relates, in general to a kit comprising a serum replacement and one or more labile factors, such as growth factors, packaged separately in the kit. It is contemplated that the kit provides advantages to improve cell growth in culture compared to cells cultured not using the kit described herein. | 2013-05-23 |
20130130374 | CELLULAR COMPOSITIONS AND METHODS FOR THEIR PREPARATION AND USE - Methods for preparing a variety of cell (e.g., hepatocyte) preparations and preparations so prepared are described. Uses of such preparations are described. In one embodiment, centrifugal elutriation is used to separate hepatocytes with preferred characteristics. Such hepatocyte preparations may be used for cryopreservation, multiple cryopreservations, in vitro assays, plating and other methods for which preparations of hepatocytes are useful. | 2013-05-23 |
20130130375 | USE OF FIBROBLAST GROWTH FACTOR FOR LINEAGE PRIMING AND DIFFERENTIATION OF PLURIPOTENT STEM CELLS - The present invention provides a method of inducing mesoderm derived cells from pluirpotent stem cells. In contrast to methods known in the art that are often designed to replicate in vivo events of mesoderm induction, the present invention provides a unique, yet simple, method whereby pluripotent stem cells are mesodermally primed in the presence of factors that concomitantly inhibit the spontaneous differentiation of endoderm and ectoderm during expansion and suspension steps. Exposure and/or adherence of primed aggregates to a extracellular matrix that promotes the commitment and survival of induced mesoderm progenitors, followed by exposure to various mesoderm associated factors, allows for the subsequent induction of such cells into terminally differentiated lineages, such as cardiomyocytes. End products of this induction system will ultimately provide an unlimited source of mesoderm-derived cell types for therapeutic and pharmacological purposes. | 2013-05-23 |
20130130376 | SCREENING METHODS, COMPOSITIONS IDENTIFIED THEREBY, TOOLS USEFUL FOR THE IDENTIFICATION THEREOF, AND CELL POPULATIONS PRODUCED THEREBY - In accordance with one aspect of the present invention, methods have been developed for identifying compositions which support the culture of defined cell populations. In accordance with another aspect of the present invention, methods have been developed for identifying compositions which promote differentiation of defined cell populations. In accordance with yet another aspect of the present invention, methods have been developed for identifying compositions which induce apoptosis of defined cell populations. In accordance with still another aspect of the present invention, methods have been developed for identifying compositions which promote cell senescence of defined cell populations. In accordance with still another aspect of the present invention, methods have been developed for identifying media which modulate the retardation of cell growth of defined cell subpopulation(s). In accordance with further aspects of the present invention, there are provided novel compositions identified by invention methods. Also provided are various uses of the novel compositions identified by invention methods, and novel cell populations produced employing same. In accordance with still another aspect of the present invention, methods have been developed for identifying compositions which support the culture of aberrant cell populations. In accordance with yet another aspect of the present invention, methods have been developed for identifying compositions which promote differentiation of aberrant cell populations. In accordance with still another aspect of the present invention, methods have been developed for identifying compositions which induce apoptosis of aberrant cell populations. | 2013-05-23 |
20130130377 | NOVEL SIRNA STRUCTURE FOR MINIMIZING OFF-TARGET EFFECTS CAUSED BY ANTISENSE STRANDS, AND USE THEREOF - The present invention relates to a novel siRNA structure and the use thereof, and more particularly to a double-stranded siRNA molecule comprising an antisense strand and a sense strand, wherein the siRNA molecule has at least one single nucleotide bulge formed by introducing a single nucleotide into the antisense strand, particularly at position 2 from the 5′ end, and to a method of using the same to silence a target gene. The siRNA molecule of the invention shows high target gene silencing efficiency while minimizing off-target effects caused by the antisense strand, and thus has improved target selectivity. Accordingly, the siRNA molecule of the invention can be substituted for conventional siRNA molecules and can be widely be used in siRNA-based gene silencing techniques, including gene therapy. | 2013-05-23 |
20130130378 | OLIGONUCLEOTIDES COMPRISING ACYCLIC AND ABASIC NUCLEOSIDES AND ANALOGS - This invention relates to acyclic and abasic nucleosides and oligonucleotides prepared therefrom. For instance, oligonucleotides can be prepared having one or more of the following formulas (I-III):, or isomers thereof. | 2013-05-23 |
20130130379 | AMINO ACID SEQUENCES DIRECTED AGAINST GPCRS AND POLYPEPTIDES COMPRISING THE SAME FOR THE TREATMENT OF GPCR-RELATED DISEASES AND DISORDERS - The present invention relates to amino acid sequences that are directed against G-protein coupled receptors (GPCRs), as well as to compounds or constructs, and in particular proteins and polypeptides, that comprise or essentially consist of one or more such amino acid sequences. The invention also relates to nucleic acids encoding such amino acid sequences and; to methods for preparing such amino acid sequences and polypeptides; to host cells expressing or capable of expressing such amino acid sequences or polypeptides; to compositions, and in particular to pharmaceutical compositions, that comprise such amino acid sequences, polypeptides, nucleic acids and/or host cells; and to uses of such amino acid sequences or polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes. | 2013-05-23 |
20130130380 | PLATFORM OF DENDRITIC CELL (DC)-BASED VACCINATION - The present invention discloses novel dendritic cell maturation-inducing cytokine cocktails, and methods for inducting type-1 polarized dendritic cells in serum-free conditions which enhance the desirable properties of DC1s generated in serum-supplemented cultures. The invention further discloses methods and systems using IFNγ and other ligands of the IFNγ receptor, in combination with IFNα (or other type I interferons), poly I:C, and other IFNα (and IFNβ) inducers to enhance the IL-12-producing properties of dendritic cells. More specifically, the present invention discloses type-1 polarized dendritic cells that have a unique combination of a fully-mature status and an elevated, instead of “exhausted”, ability to produce IL-12p70. allows for the generation of fully-mature DC1s in serum-free AIM-V medium. The invention discloses systems that use the foregoing products and methods to facilitate the clinical application of DC1-based vaccines and the identification of novel factors involved in the induction of Th1 and CTL responses by DC1. | 2013-05-23 |
20130130381 | Method for obtaining a population of stromal progenitor cells - The present invention relates to a technique for obtaining stromal progenitor cells (SPC) from adipose tissue (AT) using incubation of very small volumes of AT with an enzyme solution, that can obtain SPC for autologous SPC-based medical applications to a greater number of individuals, possibly having a lower Body Mass Index (B.M.I), i.e. lower than | 2013-05-23 |
20130130382 | AUTOSERUM-CONTAINING BONE MARROW CELL CULTURE SYSTEM, AUTOSERUM-CONTAINING BONE MARROW CELL CULTURE METHOD, AND METHOD FOR PRODUCING MEDICINAL COMPOSITION COMPRISING AUTOSERUM-CONTAINING CULTURED BONE MARROW CELLS AS ACTIVE INGREDIENT - To provide an autoserum-containing bone marrow cell culture system, whereby bone marrow cells, which are collected from a subject without using an anticoagulant, are subjected to an anticoagulation treatment using a medium in a liquid-tight state, cultured and then further cultured using the serum of said subject which is prepared in a liquid-tight state; an autoserum-containing bone marrow cell culture method; and a method for producing a medicinal composition which comprises, as the active ingredient, autoserum-containing cultured bone marrow cells. [Solution] An autoserum-containing bone marrow cell culture system for culturing bone marrow cells, which are collected from a subject without using an anticoagulant, using the serum of said subject, said system comprising a bone marrow cell suspension-storing device, a collected blood-storing device, an autoserum-acquiring device, and a bone marrow cell-culturing device. | 2013-05-23 |
20130130383 | ULTRAHIGH SURFACE AREA SUPPORTS FOR NANOMATERIAL ATTACHMENT - The present invention is directed to a hierarchical structure characterized by ultrahigh surface area comprising: a solid substrate; an intermediate layer; and at least one plurality of nanoscale attachments that are strongly bonded to the intermediate layer. Also disclosed is a method of fabricating a hierarchical structure comprising: selecting and preparing a parent substrate, wherein the preparing may optionally include cleaning or activation; modifying the substrate surface to form an intermediate layer; attaching at least one plurality of nanoscale attachments, wherein the nanoscale attachments are selected from nanotubes, nanoparticles, or combinations thereof, onto the intermediate layer; optionally attaching a second plurality of nanoscale attachments, wherein the nanoscale attachments are selected from nanotubes, nanoparticles, or combinations thereof, onto the first plurality of nanoscale attachments and intermediate layer. | 2013-05-23 |
20130130384 | TEMPERATURE RESPONSIVE SHEET THAT DISPLAYS REVERSIBLE PROPERTIES AND CELL SHEET PRODUCTION METHOD USING SAME - A temperature responsive sheet is disclosed that comprises a chemically modified water-soluble elastin obtained by N-acylating at least some of the primary amines and secondary amines contained in a high molecular weight water-soluble elastin molecule and coupling some or all of carboxyl groups contained in the molecule with a glycine alkyl ester. Also is disclosed a process for producing a cell sheet that comprises preparing above the temperature responsive sheet a film that functions as a scaffold for animal cells, culturing specific cells on the film so as to prepare a cell sheet, and subsequently separating the cell sheet and the temperature responsive sheet comprising the chemically modified water-soluble elastin under conditions of no greater than the culturing temperature for the cells. | 2013-05-23 |