20th week of 2015 patent applcation highlights part 45 |
Patent application number | Title | Published |
20150132727 | Set of Blocks and Method For Teaching Mathematics - A set of education blocks and method for teaching mathematics through equation checking includes a plurality of polygonal-shaped numeral blocks each having a plurality of faces, wherein a top face includes a numeral and a bottom face opposite the top face includes a dot pattern corresponding to the numeral on the top face. The set of blocks includes a plurality of polygonal-shaped operator blocks each including a plurality of faces, a top face presenting an arithmetic operation symbol. Selected numeral and operator blocks are selectively arranged to form an equation, an inversion of the numeral blocks revealing respective dot patterns to confirm the equation's correctness. The method of teaching mathematics includes each numeral block associating a numeral with its corresponding dot pattern representation, textual representation in one or more languages, and a color indicia indicative of the numeral being even, odd, or prime. | 2015-05-14 |
20150132728 | TRANSPORTABLE FIRE TRAINING APPARATUS AND METHOD - A transportable fire training apparatus includes a trailer frame having a rear raised section at a rear end of the trailer frame and an unraised section at a middle portion of the trailer frame, a shell attached to the trailer frame and partitioned into a plurality of training rooms, a rear multi-axle wheel set disposed below, and attached to, the rear raised section of the trailer frame via an air ride suspension system that raises the trailer frame relative to the a rear multi-axle wheel set in response to pressurization of the air ride suspension system. The unraised section of the trailer frame rides substantially above the ground when the air ride suspension system is in a pressurized state and rests on the ground when the air ride suspension system is in an unpressurized state. A method for deploying and using the transportable fire training apparatus is also disclosed herein. | 2015-05-14 |
20150132729 | System and method for a Reducing Recidivism - This invention describes a journaling and graphing application that easily collects thoughts, data and feelings and relates them to a support community and flexible calendar. The application then presents data relating to events, challenges, and attitudes as either conducive to recovery, or antithetical to recovery and encourages good behavior through feedback, notifications and game dynamics. The application is comprised of six primary feature sets—journaling, community networking, polling and alerting, gamification, care paradigm and feedback. The system uses and co-occurrence and correlation of events over time to establish a predictive analysis of outcomes and to flag good and bad influences. | 2015-05-14 |
20150132730 | SENSOR MEMORY KITS AND METHOD FOR IMPROVING MEMORY - A kit for improving or stimulating a human's memory comprises a set of sensible materials for the human to sense and a set of instructions for using the kit. The set of instructions comprises the steps of: (a) presenting the human with a material from a set of sensible materials; (b) instructing the human to sense the material; (c) instructing the human to identify the material; (d) recording the responses; (e) eliciting from the human at least a memory associated with the material; (f) recording the memory elicited in step (e); (g) instructing the human to sense the same material that the human is instructed to sense in the step (b); and (h) instructing the human to identify the material the human is instructed to sense in the step (g). | 2015-05-14 |
20150132731 | PHYSICAL THERAPY SYSTEM - A technique of attempting to help a person successfully complete physical therapy. The technique includes at least the following steps: providing, via a first computing device including at least tangible computing elements, options for one or more physical therapy exercises to the first person; providing, via the first computing device, instructions for performing the physical therapy exercises to the first person; receiving, by the first computing device, information from the first person about performance of the physical therapy exercises; and transmitting the information from the first computing device to a second computing device used by a second person. Also, systems that perform the technique. | 2015-05-14 |
20150132732 | ADVANCED SURGICAL SIMULATION - A system for training a clinician in energy-based surgical techniques that advantageously does not require the simulated tissue to be electrically conductive is provided. The simulated tissue comprises at least two materials. A heat generator is configured in the shape of a medical instrument typically encountered in energy-based surgical procedures such as electrosurgery or electrocautery. The instrument delivers sufficient heat to melt at least one of the materials in order to simulate energy-based surgical techniques such as excising target material. The at least two materials are configured in the simulated tissue such that their relative thermoplasticity defines a predetermined surgical pathway of a desired clinical outcome. | 2015-05-14 |
20150132733 | POSTPARTUM UTERUS MODEL - The invention provides a simulator for training of personnel for immediate postpartum medical treatment, distinctive in that the simulator comprises a representation of an immediate postpartum stage uterus, a cervix, a vagina and a resilience member, the uterus is arranged at an angle to the vagina and the resilience member is arranged for allowing said angle to be straightened out by pressing horizontally forward on the uterus. The invention also provides a simulator for training of personnel for immediate postpartum medical treatment, distinctive in that the simulator comprises a representation of an immediate postpartum stage uterus, a cervix and a vagina, the uterus comprises a transparent top or intermediate layer allowing visual observation, and preferably a removable or foldable non-transparent top layer over the transparent layer. | 2015-05-14 |
20150132734 | MOLECULAR MODELING SYSTEM INCLUDING MULTIPLE PI-BOND EXCLUSIONARY FEATURES - A molecular modeling kit provides a system for molecular modeling that represents exclusion between resonance structures in a molecule and permits their rotation, providing an informative tool for describing resonance in a classroom or other learning environment. The kit includes atom modeling components and bond connector components that are connected together to form models of molecules. The atom modeling components or the bond connector components include a mechanical exclusion feature that prevents simultaneous connection of two bond connector components to represent two pi-bonds formed with a single atom in a single plane. | 2015-05-14 |
20150132735 | PRESENTATION REHEARSAL - Some implementations may include a computing device to generate a presentation and enable rehearsing delivery of the presentation. The presentation may include multiple slides, with each slide having one or more visual elements, such as text or graphics. One or more notes sections may be created. Each of the one or notes sections may correspond to a visual element of the one or more visual elements. Time targets associated with each of the one or more visual elements may be received. During rehearsal, visual elements may be highlighted based on the time targets and/or a position of the visual elements on a slide may be identified based on the time targets. | 2015-05-14 |
20150132736 | TEACHING AID - A aid for reading a picture book aloud to a group of students, while showing the pictures to the students. A teaching aid showing information about the content of the pages in a book, is mounted on the open covers on the book, facing away from the pages, so that the teacher can show the contents of the pages of a book to the students, and, while being positioned behind the book, and can still know what is being shown. | 2015-05-14 |
20150132737 | SYSTEM FOR KEEPING ALIVE AND TRANSPORTING SKIN BIOPSIES AND APPLICATIONS OF SAID SYSTEM - Disclosed is an in vitro or ex vivo method for preserving and/or keeping alive mammalian, preferably human skin biopsies, enabling to transport it and, if applicable, to culture it. Also disclosed is a skin biopsy thus preserved and obtained by such a method and relates to its use as a model especially in a kit for screening or selecting cosmetic or therapeutic compounds. | 2015-05-14 |
20150132738 | Method For Identification Of Non-Hematogeneous Karocytes Enriched From Body Fluid Of Humans Or Animals - Disclosed is a method for identification of non-hematogenous karyocytes enriched from body fluid of humans or animals, wherein immunofluorescent staining of antibodies against human leukocytes and fluorescence in situ hybridizationof chromosome are carried out synchronously to distinguish hematogenous karyocytes and the interference produced by the hematogenous karyocytes during the judgement on tumour cells is removed effectively, thus achieving efficient and rapid enrichment and extraction from human body fluid of non-hematogenous rare cancer cells originating from the epithelium or not originating from the epithelium and effective identification. The method provided by the present invention does not need to lyse and break red blood cell and without influencing the targeted cell in human body fluid, thus the original cell shape is maintained very well, which is convenient for the subsequent identification and judgement of cells. | 2015-05-14 |
20150132739 | MULTILAYERED BLOOD PRODUCT - A blood product ( | 2015-05-14 |
20150132740 | LANTHANUM-BASED CONCENTRATION AGENTS FOR MICROORGANISMS - A concentration agent for microorganisms is provided that contains both lanthanum and carbonate. Additionally, articles that include the concentration agent and methods of concentrating a microorganism using the concentration agent are provided. | 2015-05-14 |
20150132741 | EXTRACTION CONTROL FOR DNA - The present invention relates to compounds for use in the control of extraction procedures, particular in connection with nucleic acid material for use in PCR and more preferably real time PCR (quantitative PCR), The extraction control according to the present invention is based on non-pathogenic bacterial material which can be produced at low cost, in large quantities and which has good stability. | 2015-05-14 |
20150132742 | Microfluidic Devices Formed From Hydrophobic Paper - Microfluidic devices fabricated from paper that has been covalently modified to increase its hydrophobicity, as well as methods of making and using thereof are provided herein. The devices are typically small, portable, flexible, and both easy and inexpensive to fabricate. Microfluidic devices contain a network of microfluidic components, including microfluidic channels, microfluidic chambers, microwells, or combinations thereof, designed to carry, store, mix, react, and/or analyze liquid samples. The microfluidic channels may be open channels, closed channels, or combinations thereof. The microfluidic devices may be used to detect and/or quantify an analyte, such as a small molecules, proteins, lipids polysaccharides, nucleic acids, prokaryotic cells, eukaryotic cells, particles, viruses, metal ions, and combinations thereof. | 2015-05-14 |
20150132743 | PROXIMITY ASSAYS FOR DETECTING NUCLEIC ACIDS AND PROTEINS IN A SINGLE CELL - Methods and reagents for detection and analysis of nucleic acids and proteins using proximity extension assays. | 2015-05-14 |
20150132744 | METHODS OF DETECTING CELLS LATENTLY INFECTED WITH HIV - Disclosed are methods of detecting and isolating CD4 | 2015-05-14 |
20150132745 | METHODS AND COMPOSITIONS FOR GENERATING REFERENCE MAPS FOR NANOPORE-BASED POLYMER ANALYSIS - The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis. | 2015-05-14 |
20150132746 | SAMPLING MEDIUM - Provided herein is technology relating to the collection of biological samples and particularly, but not exclusively, to compositions, methods, and uses related to using a biopolymer substrate to collect biological samples for analysis. | 2015-05-14 |
20150132747 | Treatment of Neurodegeneration and Neuroinflammation - Methods of treating a subject having a condition characterized by at least one of neurodegeneration and neuroinflammation are provided. Methods of reducing astrogliosis in a subject having a condition characterized by increased astrogliosis are also provided. Methods of providing neuroprotection to a subject in need thereof are also provided. | 2015-05-14 |
20150132748 | INTEGRATED MICROFLUIDIC AND SOLID STATE PYROSEQUENCING SYSTEMS - The invention provides for sequencing a nucleic acid molecule based on the detection of base incorporation by the release of pyrophosphate (PPi) using a new enzyme system comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and its substrate ADP-glucose. | 2015-05-14 |
20150132749 | METHODS AND KITS FOR ISOLATING NUCLEIC ACID FROM AN ORGANISM - The invention generally relates to methods and kits for isolating nucleic acids from an organism. In certain embodiments, methods of the invention involve contacting a plurality of lytic enzymes to an organism, thereby lysing a cell wall of the organism to release the nucleic acid, and introducing at least one agent to separate the nucleic acid from the lysed cells, thereby isolating the nucleic acid. | 2015-05-14 |
20150132750 | Compositions and Methods for Oxygenation of Nucleic Acids Containing 5-Methylpyrimidine - 5-methylpyrimidine oxygenases and their use in the modification of nucleic acids are described. | 2015-05-14 |
20150132751 | Detecting Single Nucleotide Polymorphism Using Overlapped Primer and Melting Probe - Methods for the detection of the presence or absence of a single nucleotide polymorphism (SNP) in a target nucleic acid in a biological or non-biological sample are described. The methods can include performing an amplifying step using primers, a hybridizing step utilizing a melting probe, and a detecting step, wherein a decreasing shift in the predefined melting temperature of the melting probe is indicative of the presence of the SNP in the sample and wherein the absence of a decreasing shift in the predefined melting temperature of the melting probe is indicative of the absence of the SNP in the sample. | 2015-05-14 |
20150132752 | METHOD FOR DETERMINING HLA-A*24 GROUP - The method of the present invention is a method for determining an HLA-A*24 group, which includes determining the HLA-A*24 group on the basis of the results of the typing of (a) a 211st base and (b) a 395th base, in which A (adenine) in the initiation codon for an HLA gene is defined as the 1st base in the genomic DNA of a test subject. | 2015-05-14 |
20150132753 | MICROFLUIDIC DEVICES FOR MULTI-INDEX BIOCHEMICAL DETECTION - In one aspect, a microfluidic device for multiple reactions is provided, which comprises a reaction channel comprising multiple reaction chambers connected to a closed chamber or an elastic balloon outside of the microfluidic device, wherein a wall of the closed chamber is an elastic membrane; and a control channel comprising an elastic side wall, wherein the intersections between the side wall of the control channel with the reaction channel form multiple pneumatic microvalves. In another aspect, a method for conducting multiple reactions using the microfluidic device is provided, which comprises: a) filling the reaction chambers with a sample; and b) applying pressure to the control channel to expand the elastic side wall of the control channel, wherein the expanded elastic side wall forms a pneumatic microvalve that separates the reaction chambers. | 2015-05-14 |
20150132754 | METHOD FOR INCREASING ACCURACY IN QUANTITATIVE DETECTION OF POLYNUCLEOTIDES - Disclosed is a method for improving the sensitivity and accuracy of quantitative detection of polynucleotides in a sample, such a clinical specimen, by a method that utilizes a two- or three-step process of tagging/labeling target molecules and adding an adapter sequence for adding a universal primer for efficient amplification of targets while decreasing target amplification bias. When combined with the step of statistically correcting for sequencing errors, the method can significantly increase the accuracy of quantitative detection of polynucleotides in a sample. | 2015-05-14 |
20150132755 | MULTIWELL PLATE - An assembly for processing a sample is provided. The assembly comprises a first body having a plurality of spaced-apart conduits and a second body having a plurality of chambers wherein each conduit is fluidically connected to a separate chamber. The assembly forms a plurality of liquid flow paths, each flow path comprising a conduit and a chamber. An analyte capture element is detachably attached to a conduit and is in fluidic communication with the liquid flow path of the conduit. Optionally, the assembly further may comprise a third body comprising a plurality of reservoirs. The assembly can be used to process a liquid sample for detecting an analyte. | 2015-05-14 |
20150132756 | POLYMERASE IDLING METHOD FOR SINGLE MOLECULE DNA SEQUENCING - A method for sequencing a nucleic acid is provided. In certain embodiments the method comprises obtaining a duplex comprising a nucleic acid and a primer, wherein the primer has a nuclease resistant 3′ end, combining the duplex with a chain terminator nucleotide and a proof-reading polymerase to produce a reaction in which the polymerase idles on the added chain terminator nucleotide, identifying the chain terminator nucleotide added to the end of the primer; and adding a nuclease-resistant nucleotide to the end of the primer after the polymerase has idled on and removed the added chain terminator nucleotide, thereby producing a duplex comprising the template and an extended primer that has a nuclease resistant 3′ end. | 2015-05-14 |
20150132757 | METHOD OF DETECTING AND IDENTIFYING CIRCULATING ANTIGENS IN HUMAN BIOLOGICAL SAMPLES - Disclosed herein is a method of detecting and identifying antigens that are shed into human bodily fluids during infection. The disclosed method allows circulating antigens associated with a particular infection to be detected within minutes or hours from testing as compared to days required with the current methods. Methods of identifying diagnostic indicators/targets for a given condition or disease are disclosed which include immunizing a verterinary subject with biological fluids obtained from a human infected with particular antigens to identify diagnostic targets for immunoassay. Also disclosed are methods of diagnosing and monitoring a | 2015-05-14 |
20150132758 | MAGNETIC NANOCOMPOSITE RETRIEVAL OF NUCLEOTIDE SEQUENCE - Disclosed is a process for retrieval of nucleotide sequence. The process includes mixing iron chloride tetrahydrate with iron (III) chloride hexahydrate in solution; adding ammonium hydroxide to the mixture and stirring to form maghemite nanoparticles; stirring the maghemite nanoparticles in a solution with an inorganic acid, a surfactant and a monomer precursor of a conducting polymer; initiating polymerization of the monomer by adding the inorganic acid and an oxidizing agent to the stirred solution and further stirring to yield Polyaniline/maghemite nanocomposites; adding the nanocomposites to an first aqueous solution of the nucleotide sequence and stirring so as to electrostatically interact the nanocomposites with the nucleotide sequence; and weakening the electrostatic interaction between the nanocomposite and the nucleotide sequence to recover the nanocomposite independently of the nucleotide sequence. | 2015-05-14 |
20150132759 | SOLID SUPPORT ASSAY SYSTEMS AND METHODS UTILIZING NON-STANDARD BASES - Solid support assays using non-standard bases are described. A capture oligonucleotide comprising a molecular recognition sequence is attached to a solid support and hybridized with a target. In some instances, the molecular recognition sequence includes one or more non-standard bases and hybridizes to a complementary tagging sequence of the target oligonucleotide. In other instances, incorporation of a non-standard base (e.g., via PCR or ligation) is used in the assay. | 2015-05-14 |
20150132760 | METHODS OF DETECTING CHARCOT-MARIE TOOTH DISEASE TYPE 2A - Methods are described for screening a subject for risk of Charcot-Marie-Tooth Disease Type 2A or for diagnosing Charcot-Marie-Tooth disease or a predisposition for developing Charcot-Marie-Tooth disease in a subject, by detecting the presence or absence of a mutation in the mitofusin gene in a biological sample collected from the subject. Methods are also described for detecting the presence of a genetic polymorphism associated with Charcot-Marie-Tooth Disease Type 2A in a sample of patient nucleic acid, by amplifying a mitofusin gene sequence in the patient nucleic acid to produce an amplification product; and identifying the presence of a Charcot-Marie-Tooth Disease Type 2A associated polymorphism in the amplification product. | 2015-05-14 |
20150132761 | METHOD FOR RAPIDLY EVALUATING PERFORMANCE OF SHORT INTERFERING RNA WITH NOVEL CHEMICAL MODIFICATIONS - It is an object of the instant invention to provide a method for the rapid evaluation of novel sugar modifications to be used in siRNA synthesis including the rapid evaluation of chemical modification patterns within the siRNA to effectuate increased stability and ultimately increased efficacy of a siRNA therapeutic. It is a further object of the instant invention to provide novel nucleosides useful for siRNA therapy. | 2015-05-14 |
20150132762 | METHOD AND APPARATUS FOR SHEARING OF GENOMIC MATERIAL USING ACOUSTIC PROCESSING - Method and apparatus for controlling acoustic treatment of a sample including a liquid. A processing volume in which the sample is acoustically treated may be controlled, e.g., by using a bead positioned in the sample that helps to enhance the shearing effects of acoustic energy on genomic fragments. | 2015-05-14 |
20150132763 | COMPOSITIONS AND METHODS FOR IDENTIFICATION OF A DUPLICATE SEQUENCING READ - The present invention provides methods, compositions and kits for detecting duplicate sequencing reads. In some embodiments, the duplicate sequencing reads are removed. | 2015-05-14 |
20150132764 | BAG3 AS BIOCHEMICAL SERUM AND TISSUE MARKER - The present invention concerns the field of diagnostic biological markers. Specifically the invention relates to BAG3 RNA or a fragment thereof for use as biological markers for the diagnosis of a pathological state. | 2015-05-14 |
20150132765 | SYSTEM AND METHOD FOR MEASURING FLOURESCENCE OF A SAMPLE - The present disclosure provides a system and a method for measuring fluorescence of a sample. The sample may be a polymerase-chain-reaction (PCR) array, a loop-mediated-isothermal amplification array, etc. LEDs are used to excite the sample, and a photodiode is used to collect the sample's fluorescence. An electronic offset signal is used to reduce the effects of background fluorescence and the noises from the measurement system. An integrator integrates the difference between the output of the photodiode and the electronic offset signal over a given period of time. The resulting integral is then converted into digital domain for further processing and storage. | 2015-05-14 |
20150132766 | IMAGING CELL SORTER - The present invention provides a cell enrichment/purification device having a function of continuously enriching cells, a function of locating the cells in a particular area of a flow path in a continuous array after the cell enrichment, a function of recognizing the shape of the cells and fluorescence emission from the cells at the same time in units of one cell based on an image, and a function of recognizing the cells based on the information on the shape and fluorescence emission to separate/purify the cells. | 2015-05-14 |
20150132767 | ERG/TFF3/HMWCK TRIPLE IMMUNOSTAIN FOR DETECTION OF PROSTATE CANCER - This invention relates to a triple immunostaining assay (ERG/TFF3/HMWCK) for sensitive and specific detection of prostate cancer. Positive staining for at least one of ERG or TFF3 combined with negative staining of HMWCK in a sample such as prostate tissue sample is indicative of cancer. | 2015-05-14 |
20150132768 | Anti-Pancreatic Cancer Antibodies - Described herein are compositions and methods of use of anti-pancreatic cancer antibodies or fragments thereof, such as murine, chimeric, humanized or human PAM4 antibodies. The subject antibodies show a number of novel and useful therapeutic characteristics, such as binding with high specificity to pancreatic and other cancers, but not to normal or benign pancreatic tissues and binding to a high percentage of early stage pancreatic cancers. In preferred embodiments, the antibodies bind to pancreatic cancer mucins. The antibodies and fragments are of use for the detection, diagnosis and/or treatment of cancer, such as pancreatic cancer. The antibodies, such as PAM4 antibodies, bind to a PAM4 antigen that shows unique cell and tissue distributions compared with other known antibodies such as CA19.9, DUPAN2, SPAN1, Nd2, B72.3, and Le | 2015-05-14 |
20150132769 | BIOMARKER FOR DETERMINING MITOCHONDRIAL DAMAGE IN FRIEDREICH'S ATAXIA - Compositions and methods for screening for a disease or a disorder associated with a deficiency in frataxin in a subject using biomarkers for diseases or disorders associated with a deficiency in frataxin are disclosed. The compositions and methods include determining the acetylation status of mito-chondrial proteins. Also disclosed are methods of detecting progression of a disease or a disorder associated with a deficiency in frataxin in a subject and methods of monitoring effectiveness of a therapy for diseases or disorders associated with a deficiency in frataxin. | 2015-05-14 |
20150132770 | PREGNANCY-ASSOCIATED PLASMA PROTEIN-A2 (PAPP-A2) POLYNUCLEOTIDES - The present invention provides pregnancy associated plasma protein A2 (PAPP-A2), its nucleotide and amino acid sequences, antisense molecules to the nucleotide sequences which encode PAPP-A2, expression vectors for the production of purified PAPP-A2, antibodies capable of binding specifically to PAPP-A2, hybridization probes or oligonucleotides for the detection of PAPP-A2-encoding nucleotide sequences, genetically engineered host cells for the expression of PAPP-A2, and methods for screening for pathologies in pregnant and non-pregnant patients. Methods for screening for altered focal proliferation states in pregnant and/or non-pregnant patients, which include detecting levels of PAPP-A2, are also described. | 2015-05-14 |
20150132771 | COMBINATION OF SPLA2 ACTIVITY AND LP(A) CARDIOVASCULAR RISK FACTORS FOR THE DIAGNOSIS/PROGNOSIS OF A CARDIOVASCULAR DISEASE/EVENT - The present invention related to a method of identifying a subject having or at risk of having or developing a cardiovascular disease and/or a cardiovascular event, comprising: —measuring, in a sample obtained from said subject, at least two cardiovascular risk factors: a) s PLA2 activity and b) Lipoprotein(a), —combining said measurements, the combined value of s PLA2 activity and Lp(a) being indicative of having or a risk of having or developing a cardiovascular disease and/or cardiovascular event. | 2015-05-14 |
20150132772 | Determination of Neutrophil Gelatinase-Associated Lipocalin (NGAL) as a Diagnostic Marker for Renal Disorders - Methods for diagnosing renal disorders by measuring human neutrophil gelatinase-associated lipocalin (NGAL) are provided. | 2015-05-14 |
20150132773 | IDENTIFICATION OF ATYPICAL ANTIBODIES IN HUMAN BLOOD AND BLOOD PRODUCTS - A method identifies atypical antibodies in blood or blood product manufacturing processes that may produce false positives in quality control testing on intermediate or final products. | 2015-05-14 |
20150132774 | FLUORESCENT PROTEIN VOLTAGE SENSORS FOR MEASURING MEMBRANE POTENTIAL AND IMAGING HIGH-FREQUENCY NEURONAL ELECTRICAL ACTIVITY - Fluorescent protein voltage sensors for measuring membrane potential and imaging high-frequency neuronal electrical activity are disclosed. In particular, the invention relates to engineered protein voltage sensors that comprise a voltage-sensing domain comprising four transmembrane domains linked to a circularly permuted fluorescent protein, which is inserted into the extracellular loop between the third (S3) and fourth (S4) transmembrane segments of the voltage-sensing domain. Such fluorescent protein voltage sensors can be used for measuring the electrical activity of neurons, including single action potentials, trains of action potentials, and subthreshold potential changes and, in particular, for imaging high-frequency neuronal electrical activity. Additionally, fluorescent protein voltage sensors can be used for a variety of other purposes, including measuring the membrane potential of any cell, including other excitable cells such as cardiac cells and endocrine cells, and for screening agents that target ion channels for their effects on membrane potential. | 2015-05-14 |
20150132775 | METHOD AND DEVICE FOR DETERMINING CONCENTRATION, CROSSTALK AND DISPLACEMENT FLUORESCENCE CROSS CORRELATION SPECTROSCOPY - The present invention provides a FCCS method for determining the concentration and/or the diffusion coefficient of at least a first labeled species, a second labeled species and/or a complex between said first and second labeled species, in a system, wherein the method comprises the steps of determining a cross-talk parameter K, wherein K is the ratio between the brightness of the first labeled species and the second labeled species at the centre of each focus, as detected for both species in the channel for detecting the second labeled species; using the cross talk parameter K for determining a displacement parameter r | 2015-05-14 |
20150132776 | KITS, COMPOSITIONS AND METHODS FOR DETECTING A BIOLOGICAL CONDITION - The present invention provides kits, apparatus and methods for determining a biological condition in a mammalian subject, the method includes incubating a specimen from a patient with at least one composition in a kit for a predetermined period of time to form at least one reaction product, when the subject has said biological condition, and receiving an indication of the at least one reaction product responsive to at least one reporter element in the kit thereby providing the indication of the biological condition in the subject. | 2015-05-14 |
20150132777 | NON-HEATING DETECTION METHOD FOR DERMATOPHYTE - The present invention provides a method of detecting dermatophyte, which does not require a complicated operation such as an enzyme treatment and a heat treatment. | 2015-05-14 |
20150132778 | METHODS AND KITS FOR DIAGNOSING HEPARIN-INDUCED THROMBOCYTOPENIA - Provided herein are methods for diagnosing heparin-induced thrombocytopenia (HIT) in a subject. The methods comprise (a) contacting a PF4-heparin complex with a biological sample from the subject; (b) contacting the composition of part (a) with an HIT-like antibody; and (c) measuring the amount of bound HIT-like antibody. In one embodiment, a significant decrease in the amount of bound HIT-like antibody in the composition of part (b) as compared to a control level is indicative of a diagnosis of HIT in the subject. Also provided are assay kits for performing the methods of the invention. | 2015-05-14 |
20150132779 | Method for Determining Ubiqutin Chain Length - Protein ubiquitylation, an essential post-translational modification, regulates almost every cellular process including protein degradation, protein trafficking, signal transduction, and DNA damage response in eukaryotic cells. The diverse functions of ubiquitylation are thought to be mediated by distinct chain topologies resulting from eight different ubiquitin linkages, chain lengths, and complexities. Currently, ubiquitin linkages are generally thought to be a critical determinant of ubiquitin signaling. However, ubiquitin chain lengths, another key element of ubiquitin signaling, have not been well documented especially in vivo situation during past three decades from the discovery of ubiquitin. The reason of this was simply because no method has been available for determination of ubiquitin chain length in endogenous ubiquitylated substrates. In the present invention, a practical technique for determining the actual length of substrate-attached polyubiquitin chains from biological samples is established. Using the method, the mean length of substrate-attached polyubiquitin chains was determined and the robustness of ubiquitin chain length regulation in cells is investigated. The following is a summary of findings in this invention: 1. A method for determining ubiquitin chain length was developed and this method was named ‘ubiquitin protection from trypsinization’ (Ub-ProT). 2. Using Ub-ProT, it was determined that the mean length of substrate-attached ubiquitin chains is in the dimer to decamer range. 3. By quantitative proteomics, it was found that the mean lengths of five major types of ubiquitin chains can be divided into two groups. 4. Proteasome-inhibition did not alter the mean length of substrate-attached polyubiquitin chains, indicating that cells have a robust system for regulating ubiquitin chain length. | 2015-05-14 |
20150132780 | MELITTIN PEPTIDE CONJUGATES AND METHODS EMPLOYING SAME - Methods and reagents are disclosed for conducting assays for IgE specific for honey bee venom allergen. A reagent comprises a conjugate of a small molecule linked to a terminal glycine amino acid of a synthetic 26 amino acid melittin peptide. In the method a combination is provided that comprises a sample and the aforementioned reagent. The combination is subjected to conditions for binding of IgE specific for honey bee venom allergen to the reagent to form a complex. One or both of the presence and amount of the complex is detected and related to one or both of the presence and amount in the sample of IgE specific for honey bee venom allergen. | 2015-05-14 |
20150132781 | MULTIPLEXED CHROMATOGRAPHY-IMMUNOASSAY METHOD FOR THE CHARACTERIZATION OF CIRCULATING IMMUNE COMPLEXES - Thus, herein is reported a method for analyzing/characterizing circulating immune complexes (CICs) formed in vivo comprising a size-exclusion chromatography of a sample obtained from a mammal to which the drug had been administered at least once for determining the weight/size of the immune complexes, optionally a second non-SEC chromatography, and at least one immunoassay, whereby the immune complex is characterized by the correlation of the immune complex size and the immunoassay result/read-out. Also reported herein is the use of a method as reported herein for determining a correlation to altered pharmacokinetics, for determining loss or reduction of efficacy, for determining neutralization of natural counterparts of the drug, for determining immune and hypersensitivity reactions, including serum sickness/type III hypersensitivity reaction/immune complex-mediated disease. | 2015-05-14 |
20150132782 | METHOD FOR DIAGNOSING CANCER BY DETECTING GPC3 - Provided is a method for diagnosing cancer by detecting a novel cancer marker. Cancer can be diagnosed by detecting soluble glypican 3 in a test sample. | 2015-05-14 |
20150132783 | Neuroprotective Chemicals and Methods for Identifying and Using Same - Provided herein are methods for identifying a compound having cell-protective (e.g., neuroprotective) activity. Compounds identified therefrom are also provided. These compounds can be used to treat various diseases, disorders, or conditions associated with, for example, unwanted cell death. | 2015-05-14 |
20150132784 | METHODS AND KITS FOR DETECTING A XEROPHILIC MICROORGANISM OR AN OSMOPHILIC MICROORGANISM - A method for detecting a xerophilic microorganism is provided. The method comprises providing a sample to be tested, an aqueous diluent comprising about 1.0 to about 10.2 weight percent glycerol, and a thin film culture device comprising a cold water-reconstitutable medium to facilitate the growth of a microorganism. The method further comprises mixing the sample with the aqueous diluent to form an inoculum, contacting a predefined amount of the inoculum with the reconstitutable medium to form an inoculated thin film culture device, incubating the inoculated thin film culture device for a period of time, and detecting a presence or an absence of colony of a microorganism. Kits for detecting a xerophilic microorganism according to the method are also provided. | 2015-05-14 |
20150132785 | PROTEASE ASSAY - The present invention provides a diagnostic reagent or assay for assessing the activity of a protease in vivo or in vitro and methods of detecting the presence of a cancerous or precancerous cell. The assays are comprised of two particles linked via an oligopeptide linkage that comprises a consensus sequence specific for the target protease. Cleavage of the sequence by the target protease can be detected visually or using various sensors, and the diagnostic results can be correlated with cancer prognosis. | 2015-05-14 |
20150132786 | METHOD FOR MEASURING GLYCOSYLATED HEMOGLOBIN, MEASUREMENT REAGENT, AND MEASUREMENT KIT - Described is a method for accurately and highly sensitively measuring glycated hemoglobin in a hemoglobin-containing sample without being influenced by hemoglobin. It is related to a method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising: reacting hemoglobin-containing sample with a protease in the presence of a surfactant; and then reacting the obtained reaction product with fructosyl peptide oxidase, wherein the latter reaction or both of the former reaction and the latter reaction are performed in the presence of a halogen oxide, and measuring the generated hydrogen peroxide. The method for measuring glycated hemoglobin in a hemoglobin-containing sample provided by the present invention is useful in, for example, the measurement of glycated hemoglobin useful in the diagnosis of diabetes mellitus. | 2015-05-14 |
20150132787 | METHOD FOR PRODUCING CILIARY MARGINAL ZONE-LIKE STRUCTURE - The invention provides a method for producing a cell aggregate containing a ciliary marginal zone-like structure by culturing a cell aggregate containing a retinal tissue in which Chx10 positive cells are present in a proportion of 20% or more of the tissue in a serum-free medium or serum-containing medium, each containing a substance acting on the Wnt signal pathway for only a period before the appearance of a RPE65 gene expressing cell, followed by culturing the “cell aggregate in which a RPE65 gene expressing cell does not appear” thus obtained in a serum-free medium or serum-containing medium, each not containing a substance acting on the Wnt signal pathway and so on. | 2015-05-14 |
20150132788 | ESTABLISHING THE VIABILITY OF BIOLOGICAL SAMPLES - The invention relates to a method, a use and a kit for establishing the viability (ability to live) of biological samples using ageladine A. The invention is advantageously suitable for establishing the viability of biological samples which can otherwise be dyed in an intra-cellular manner by fluorescence dyes other than ageladine A not at all or only with difficulty. The invention is found to be very particularly advantageous for establishing the viability of eggs (ovaries) of the pig whipworm which are intended to be used for the treatment or prophylaxis of specific (gastroenterological) autoimmune diseases. | 2015-05-14 |
20150132789 | Optical imaging system and methods for using the same - Aspects of the present disclosure include methods and systems for assaying a sample for an analyte. Methods according to certain embodiments include illuminating a sample with a slit-shaped beam of light, detecting light transmitted through the sample, determining absorbance of the transmitted light at one or more wavelengths and calculating concentration of the analyte based on the absorbance to assay the sample for the analyte. Systems for practicing the subject methods are also described. | 2015-05-14 |
20150132790 | SCREEN TO IDENTIFY AGENTS THAT CAN MODULATE HEME TRANSPORTER - Provided are compositions and methods related to heme transporters and high-throughput methods of identifying agents that can modulate heme transporters. An approach for identifying a modulator of a eukaryotic heme transporter involves adding a toxic heme analog and at least one test agent to a culture of cells, wherein the cells express a recombinant heterologous eukaryotic heme transporter. The cells are incubated with the toxic heme analog and the test agent for a period of time. A change in toxic effect of the toxic heme analog relative to a control is indicative that the test agent is a modulator of the eukaryotic heme transporter. Heme transporter agonists and antagonists can be identified. Also provided is a cell culture comprising a plurality of cells which express a recombinant heterologous eukaryotic heme transporter. The plurality of cells are divided into a plurality of reaction chambers, each of which may contain a test agent, and may further contain a toxic heme analog. | 2015-05-14 |
20150132791 | SAMPLE ANALYZER AND COMPUTER PROGRAM PRODUCT - A sample analyzer prepares a measurement sample from a blood sample or a body fluid sample which differs from the blood sample; measures the prepared measurement sample; obtains characteristic information representing characteristics of the components in the measurement sample; sets either a blood measurement mode for measuring the blood sample, or a body fluid measurement mode for measuring the body fluid sample as an operating mode; and measures the measurement sample prepared from the blood sample by executing operations in the blood measurement mode when the blood measurement mode has been set, and measuring the measurement sample prepared from the body fluid sample by executing operations in the body fluid measurement mode that differs from the operations in the blood measurement mode when the body fluid measurement mode has been set, is disclosed. A computer program product is also disclosed. | 2015-05-14 |
20150132792 | METHODS OF ASSAYING SENSITIVITY OF CANCER STEM CELLS TO THERAPEUTIC MODALITIES - The present invention is directed to methods of measuring the proliferative ability of individual patient cancer stem cells. The present invention provides a method for treating a cancer patient according to an assay of the individual patient's tumor's cancer stem cell sensitivity, by measuring the proliferative ability of cancer stem cells from the patient. By the methods of the present invention it is possible to treat individual cancer stem cells presented in tumor cells. Methods of detecting and enumerating cancer stem cells in hybrid spheroids comprised of fibroblasts and tumor cells are also provided by the present invention. The present invention also contemplates a method for drug and other treatment development, wherein the effects of a drug or combination of drugs or other treatments are determined on the individual patient's cancer stem cells. | 2015-05-14 |
20150132793 | METHOD FOR FILTERING A BIOLOGICAL SAMPLE - A method for filtering a sample comprising the steps of: (a) providing a sample with eukaryotic cells and containing or suspected to contain a micro-organism; (b) performing a selective lysis of the eukaryotic cells to obtain a lysed sample; (c) filtering the lysed sample obtained in step (b) through a filter arranged to retain the micro-organism; (d) washing the filter with a detergent based wash buffer to selectively solubilize proteins originating from the eukaryotic cells retained by the filter, by passing the detergent based wash buffer through the filter, to remove protein clogs from the filter in order to allow an additional step (c) of filtering said lysed sample. | 2015-05-14 |
20150132794 | METHOD OF MANUFACTURING SAMPLE CONTAINERS - A method of manufacturing a batch of sample containers optimized for culturing particular animal cells or binding particular proteins under particular growth conditions. When a customer requires a batch of sample containers, an appropriate surface is first selected by a testing process. A test container is provided which has a surface subdivided into a plurality of test areas, for example in a two-dimensional square array. Each test area has a pre-defined combination of surface properties including a micro- or nano-structure and these properties are varied from test area to test area. The animal cells or protein of interest are then tested under the particular conditions to be used. The test area that provides the ‘best’ conditions is then selected. The manufacture of a production batch of containers is then carried out, wherein the test areas in the production batch all copy the ‘best’ surface structure selected in the test. | 2015-05-14 |
20150132795 | SYSTEMS AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES - Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity. | 2015-05-14 |
20150132796 | KIT FOR DETECTING BIOFILMS - The invention relates to a kit for detecting biofilms, which is in particular compatible with the agri-food industry and comprises a biofilm staining solution containing a stain in solution in a dilution phase compatible with the agri-food industry, wherein said stain is Coomassie blue, and a cleaning solution comprising said dilution phase. | 2015-05-14 |
20150132797 | COMPOSITION, METHOD AND KIT FOR REDUCING BACKGROUND STAINING - Compositions, methods and kits are disclosed for improved staining of a cell or tissue with a dye-conjugate that binds specifically to a particular component of the cell or tissue. The compositions, methods and kits include a polymeric material that reduces non-specific binding of a dye-conjugate to components of the cell or tissue other than the particular component specifically bound by the dye-conjugate. In some embodiments, the polymeric material is a synthetic polymer or a naturally-occurring polymer that is substituted by multiple sulfate, sulfonate, phosphate and/or phosphonate groups. | 2015-05-14 |
20150132798 | INTEGRATED SEQUENTIAL SAMPLE PREPARATION SYSTEM - The invention provides an integrated sequential sample preparation system using a sequential centrifuge for preparing samples for analysis. Methods of more efficiently preparing discrete samples sequentially for subsequent analysis are also provided. The apparatus and methods for sequentially preparing discrete samples provide improved operating efficiencies over conventional preparation processes that use batch centrifugation systems. Such advantages include reducing dwell time, increasing system throughput, reducing sample preparation system footprint, and improving precision of the analytical process. The integrated sequential preparation system with the integrated sequential centrifuge further provides the capability of handling critical or STAT samples without compromising the operating efficiencies achieved by preparing discrete samples in a sequential manner. | 2015-05-14 |
20150132799 | METHODS TO INCREASE AND HARVEST DESIRED METABOLITE PRODUCTION IN ALGAE - Enhanced yields of photosynthetically fixed carbon produced by hypersaline photosynthetic algae are provided by co-culturing with a halophilic archaea. Further, methods are provided to control harvesting of desired metabolic products from hypersaline photosynthetic algae by controlling caspase activity. | 2015-05-14 |
20150132800 | Trichoderma Hydrophobin Production - The invention relates to a method for maximizing expression of a biosurfactant, such as hydrophobin, in a microorganism, in particular a | 2015-05-14 |
20150132801 | LOW ACIDIC SPECIES COMPOSITIONS AND METHODS FOR PRODUCING AND USING THE SAME - The instant invention relates to low acidic species (AR) compositions comprising a protein, e.g., an antibody, or antigen-binding portion thereof, and methods, e.g., cell culture and/or protein purification methods, for producing such low AR compositions. Methods for using such compositions to treat a disorder, e.g., a disorder in which TNFα is detrimental, are also provided. | 2015-05-14 |
20150132802 | ALKALINE FEED - A method for cultivating a bacterial cell comprising the addition of an amino acid in an alkaline solution used for pH regulation. Also an aspect is a method for producing a polypeptide comprising the steps of a) providing a bacterial cell comprising a nucleic acid encoding the polypeptide, b) cultivating the provided cell, c) adjusting the pH value during the cultivating with a basic solution comprising an amino acid, d) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide | 2015-05-14 |
20150132803 | POLYPEPTIDE FOR IMPROVING PROTEIN PRODUCTION IN MICROORGANISMS OF THE PHYLUM LABYRINTHULOMYCOTA - The present invention relates to recombinant cells and microorganisms of the phylum Labyrinthulomycota and their use in heterologous protein production. Novel promoter, terminator, and signal sequences for efficient production and, optionally, secretion of polypeptides from recombinant host cells and microorganisms are also encompassed by the present invention. | 2015-05-14 |
20150132804 | Instruments And Method Relating To Thermal Cycling - The present invention relates to a device for thermal cycling of biological samples, a heat sink used in such a device and a method. The heat sink comprises a base plate designed to fit in a good thermal contact against a generally planar thermoelectric element included in the device, and a plurality of heat transfer elements projecting away from the base plate. According to the invention, the heat transfer elements of the heat sink and arranged in a non-parallel configuration with respect to each other for keeping the temperature of the base plate of the heat sink spatially uniform during thermal cycling. | 2015-05-14 |
20150132805 | COMPOSITION FOR PRODUCING MICRORNA PRECURSORS AS DRUGS FOR ENHANCING WOUND HEALING AND PRODUCTION METHOD OF THE MICRORNA PRECURSORS - This invention generally relates to a composition and its production method useful for developing drugs and/or therapies for enhancing wound healing, in particular scarless wound healing. Particularly, the present invention teaches the essential processes necessary for producing and purifying embryonic stem cell (ESC)-specific RNA compositions, such as messenger RNAs (mRNA), microRNA precursors (pre-miRNA), and small hairpin RNAs (shRNA), which are useful for treating human diseases and lesions. | 2015-05-14 |
20150132806 | KETOREDUCTASE POLYPEPTIDES - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme including the capability of reducing 5-((4S)-2-oxo-4-phenyl(1,3-oxazolidin-3-yl))-1-(4-fluorophenyl)pentane-1,5-dione to (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize the intermediate (4S)-3-[(5S)-5-(4-fluorophenyl)-5-hydroxypentanoyl]-4-phenyl-1,3-oxazolidin-2-one in a process for making Ezetimibe. | 2015-05-14 |
20150132807 | ENGINEERED IMINE REDUCTASES AND METHODS FOR THE REDUCTIVE AMINATION OF KETONE AND AMINE COMPOUNDS - The present application provides engineered polypeptides having imine reductase activity, polynucleotides encoding the engineered imine reductases, host cells capable of expressing the engineered imine reductases, and methods of using these engineered polypeptides with a range of ketone and amine substrate compounds to prepare secondary and tertiary amine product compounds. | 2015-05-14 |
20150132808 | METHOD FOR PRODUCING 1,5-PENTAMETHYLENEDIAMINE, MUTANT LYSINE DECARBOXYLASE, METHOD FOR PRODUCING 1,5-PENTAMETHYLENE DIISOCYANATE AND METHOD FOR PRODUCING POLYISOCYANATE COMPOSITION - A mutant lysine decarboxylase is produced by replacing at least one of the amino-acids in the amino acid sequence shown in sequence ID No. 4 of the sequence listing with another amino-acid. | 2015-05-14 |
20150132809 | Systems and Methods for Enhancing Gene Expression - The present invention provides for a system for increasing the production of a compound using an artificial positive feedback loop (APFL). In some embodiments, the system diverts a compound produced in a first metabolic pathway into a second metabolic pathway in order to produce a compound of interest. | 2015-05-14 |
20150132810 | INTEGRATED PROCESSES FOR ANAEROBICALLY BIOCONVERTING HYDROGEN AND CARBON OXIDES TO OXYGENATED ORGANIC COMPOUNDS - Integrated processes are provided for the bioconversion of syngas to oxygenated organic compound with the ability to recover essential compounds for the fermentation and recycle the compounds to the fermentation. | 2015-05-14 |
20150132811 | DEACTIVATION OF URUSHIOL AND METHOD OF TREAMENT AND PREVENTION OF CONTACT DERMATITIS - The invention herein disclosed provides for compositions, methods for synthesizing said compositions, and methods for using said compositions, wherein the compositions and methods may be used to bind to and/or deactivate a poison oak oil, such as urushiol. The compositions and methods can be used to treat and/or reduce an inflammatory reaction and/or hypersensitivity to natural compounds found in poison oak, poison ivy, poison sumac, mango, lac tree, cashew nut, and Asian lacquer. | 2015-05-14 |
20150132812 | TWO-STAGE PROCESS FOR PRODUCING OIL FROM MICROALGAE - A process for production of biofuels from algae can include cultivating an oil-producing algae by promoting sequential photoautotrophic and heterotrophic growth. The method can further include producing oil by heterotrophic growth of algae wherein the heterotrophic algae growth is achieved by introducing a sugar feed to the oil-producing algae. An algal oil can be extracted from the oil-producing algae, and can be converted to form biodiesel. | 2015-05-14 |
20150132813 | BIOSYNTHETIC PATHWAYS, RECOMBINANT CELLS, AND METHODS - This disclosure describes, generally, recombinant cells modified to exhibit increased biosynthesis of pentanoic acid, methods of making such recombinant cells, and methods of inducing the cells to produce pentanoic acid. This disclosure also describes, generally, recombinant cells modified to exhibit increased biosynthesis of 2-methylbutyric acid, methods of making such recombinant cells, and methods of inducing the cells to produce 2-methylbutyric acid. | 2015-05-14 |
20150132814 | PROCESS FOR FERMENTING SUGARS CONTAINING OLIGOMERIC SACCHARIDES - Sugar mixtures containing nonfermentable oligomers are fermented in the presence of certain enzymes that depolymerise the oligomers simultaneously with the fermentation process. | 2015-05-14 |
20150132815 | PROCESSES FOR ENHANCING THE PERFORMANCE OF LARGE-SCALE, TANK ANAEROBIC FERMENTORS - Processes are disclosed for the low energy, anaerobic bioconversion of hydrogen and carbon monoxide in a gaseous substrate stream to oxygenated organic compounds such as ethanol by contact with microorganisms in a deep, tank fermentation system with high conversion efficiency of both hydrogen and carbon monoxide. Gas feed to the reactor is injected using a motive liquid to form a stable dispersion of microbubbles thereby reducing energy costs, and a portion of the off-gases from the reactor are recycled to (i) achieve a conversion of the total moles of carbon monoxide and hydrogen in the gas substrate to oxygenated organic compound of at least about 80 percent and (ii) attenuate the risk of carbon monoxide inhibition of the microorganism used for the bioconversion. | 2015-05-14 |
20150132816 | MICROBIAL PRODUCTION OF BRANCHED MEDIUM CHAIN ALCOHOLS, SUCH AS 4-METHYLPENTANOL - The invention relates to recombinant cells and their use in the production of branched medium-chain alcohols such as 4-methyl-1-pentanol. | 2015-05-14 |
20150132817 | ALCOHOLIC FERMENTATION YEAST AND METHOD FOR PRODUCING ETHANOL USING SAME - [Problem] To provide an alcoholic fermentation yeast having resistance to limonene, which is a fermentation inhibitor, and a method for producing ethanol using the alcoholic fermentation yeast. [Solution] An alcoholic fermentation yeast | 2015-05-14 |
20150132818 | GLYCOSYLATION METHOD OF ALGAE OR AGRICULTURAL BY-PRODUCTS COMPRISING HIGH-PRESSURE EXTRUSION PULVERIZATION STEP - Disclosed is a method of saccharifying biomass, such as algae or agricultural by-products by performing a high-pressure extrusion pulverization process for the biomass, such as algae or agricultural by-products, and more particularly to a method of saccharifying biomass, which includes homogenizing and crushing algae or agricultural by-products, and extruding the algae or agricultural by-products through a micro-diameter tube to pulverize the algae or agricultural by-products. Non-biodegradable polymers, such as cellulose, which is a polysaccharide included in biomass, such as algae or agricultural by-products, hemicelluloses, starch, and complex polysaccharide, are hydrolyzed at high glycosylation efficiency through an eco-friendly pretreatment process using water. | 2015-05-14 |
20150132819 | Thermococcus onnurineus MC02 and method of hydrogen production using thereof - The present invention provides | 2015-05-14 |
20150132820 | THERMOSTABLE CATALASE - An object is to efficiently produce thermostable catalase at low cost by expressing it as a recombinant protein in large quantity. | 2015-05-14 |
20150132821 | Systems and Methods for Improving Nuclease Specificity and Activity - Endonucleases play an essential role in genetic engineering and molecular biology. A major barrier to the clinical adoption of nucleases and engineered nucleases remain the overall lack of specificity and activity. Off-site cleavage, cleavage at loci other than the target loci, typically occurs resulting in mutations, unexpected gene-knockouts, or translocations. Provided herein are systems and methods for identifying the off-site cleavage loci and predicting the activity of engineered endonucleases for a given genome. It is expected that these tools and methods will be useful for designing nucleases and other related DNA binding domains (e.g. TAL effectors) for genomic therapy and engineering. | 2015-05-14 |
20150132822 | Polypeptides Having Xylanase Activity And Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2015-05-14 |
20150132823 | Alpha-Amylase Variants - The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased. | 2015-05-14 |
20150132824 | Polypeptides Having Xanthan Degrading Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xanthan degrading activity, catalytic domains and polynucleotides encoding the polypeptides and catalytic domains. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides and catalytic domains. | 2015-05-14 |
20150132825 | COMPOSITIONS AND METHODS COMPRISING SERINE PROTEASE VARIANTS - The present invention provides methods for protein engineering and serine protease variants produced there from. Specifically, the present invention provides serine protease variants having one or more substitutions as compared to a reference serine protease. In addition, the present invention provides compositions comprising these serine protease variants. In some embodiments, the present invention provides cleaning compositions comprising at least one of these serine protease variants. | 2015-05-14 |
20150132826 | CHIMERIC TRUNCATED TISSUE PLASMINOGEN ACTIVATOR (t-PA) RESIATANT TO PLASMINOGEN ACTIVATOR INHIBITOR-1 AND IMPROVED BIOCHEMICAL PROPERTIES - The present invention discloses a thrombolytic therapy for acute myocardial infarction by t-PA. A chimeric truncated form of t-PA is designed and expressed in | 2015-05-14 |