| 19th week of 2013 patent applcation highlights part 40 |
| Patent application number | Title | Published |
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| 20130115592 | MODIFIED HUMAN HEPATITIS C VIRUS GENOMIC RNA THAT CAN BE AUTONOMOUSLY REPLICATED - The present invention provides modified hepatitis C virus genomic RNA, comprising nucleotide sequences of genomic RNA portions of two or more types of hepatitis C viruses, which comprises a 5′ untranslated region, a core protein coding sequence, an E1 protein coding sequence, a p7 protein coding sequence, an E2 protein coding sequence, an NS2 protein coding sequence, an NS3 protein coding sequence, an NS4A protein coding sequence, an NS4B protein coding sequence, an NS5A protein coding sequence, an NS5B protein coding sequence, and a 3′ untranslated region, and which can be autonomously replicated. In particular, the present invention relates to modified hepatitis C virus genomic RNA, which can be autonomously replicated by substitution of the RNA sequence portion encoding NS3, NS4, NS5A, and NS5B proteins of hepatitis C virus genomic RNA with a partial RNA sequence encoding NS3, NS4, NS5A, and NS5B proteins of a JFH1 strain shown in SEQ ID NO: 1. | 2013-05-09 |
| 20130115593 | SUBSTRATES FOR CHROMOGENIC DETECTION AND METHODS OF USE IN DETECTION ASSAYS AND KITS - Embodiments of substrates and processes for chromogenic detection, and in particular pyrazolyl dihydrogen phosphate compounds, are disclosed. | 2013-05-09 |
| 20130115594 | HIGH SPECIFICITY AND HIGH SENSITIVITY DETECTION BASED ON STERIC HINDRANCE & ENZYME-RELATED SIGNAL AMPLIFICATION - The present invention relates to a molecular probe capable of high sensitivity and high specificity detection of a target nucleic acid in a sample. Also disclosed is a detection method using this probe. | 2013-05-09 |
| 20130115595 | METHOD TO DETECT REPEAT SEQUENCE MOTIFS IN NUCLEIC ACID - Methods for determining the presence or absence of expansion of CGG repeat sequence in the FMR1 gene presence or absence of expansion of CCG repeat sequence in the FMR2 gene are provided. The methods are useful in identifying an individual with normal/intermediate, versus premutation or full mutation allele of FMR1 gene and FMR2 gene due to the expansion of CGG repeats and CCG repeats in the 5′-untranslated region respectively. The methods are also useful for screening newborns for fragile X syndrome or for screening women to determine heterozygosity status with full premutation of the CCG repeat tract. The methods are also useful in estimating the premutation and full mutation carrier frequency and estimating the prevalence of FXTAS AND FXPOI in a population. The methods are simple, rapid and require small amount of sample. | 2013-05-09 |
| 20130115596 | DNA POLYMORPHISMS AS MOLECULAR MARKERS IN CATTLE - A method of predicting the phenotype of cattle through the analysis of one or more single nucleotide polymorphisms (SNPs) is described. More particularly, a method for predicting cattle temperament and behavior through the analysis of one or more single nucleotide polymorphisms (SNPs) mapped at specific regions of the bovine genome is described. | 2013-05-09 |
| 20130115597 | METHOD FOR DETECTING SPECIFIC NUCLEIC ACID SEQUENCES - The present invention relates to a method and test kit for detecting specific nucleic acid sequences, comprising the steps of: 1. matrix-dependent new synthesis of the target nucleic acid; 2. target-specific probe hybridization; and 3. detection of the hybridization event. The invention is characterized in that, in the first step, an oligonucleotide 1, which is marked by a marker 1 and is entirely or partially complementary to the target sequence, acts as a primer in the matrix-dependent new synthesis of the target nucleic acid and, in the second step, an oligonucleotide 2, which is marked by a marker 2 and, owing to its melting temperature being lower than that of the oligonucleotide 1, is not involved in the first step, partially or completely hybridizes with the DNA new synthesis product of oligonucleotide 1.The detection of the hybridization reaction can take place both fluorometrically in the form of a homogeneous assay and, for verification of the result, subsequently immunologically. The detection reaction always takes place in time after the matrix-dependent new synthesis has been carried out. | 2013-05-09 |
| 20130115598 | OLIGONUCLEOTIDE PROBE RETRIEVAL ASSAY FOR DNA TRANSACTIONS IN MAMMALIAN CELLS - Methods to measure a variety of DNA synthetic processes in live human cells by introducing and retrieving exogenous DNA probes are provided herein. Using fragments of bacterial plasmid or phage DNA, a wide array of DNA constructs may be assembled to mimic the intermediates of DNA transactions, including replication, translation synthesis, and end-joining. These DNA probes may be transfected into human cells and retrieved for mutational analysis using a modified Random Mutation Capture assay or NextGen DNA sequencing. These assays require only a small number of cells, such as might be available from biopsy material. Thus, the methods described herein may be applied to the early detection of cancer, predicting the responsiveness of individual cancers to chemotherapy, and measuring the DNA repair capacity of individuals to environmental DNA damaging agents. This approach may be automated and used for screening human populations for variations in DNA synthetic and repair activities. | 2013-05-09 |
| 20130115599 | INCREASED CIP2A EXPRESSION AND BLADDER CANCER IN HUMANS - The present invention provides a method of detecting CIP2A protein in a bladder tissue. Methods and compositions are provided herein for detecting and diagnosing bladder cancer by obtaining a bladder tissue from a human subject suspected of bladder cancer, followed by detecting CIP2A protein or mRNA levels in the bladder tissue using Western blot analysis or ELISA to specifically detect CIP2A protein or qRT-PCR to specifically detect CIP2A mRNA. The present method permits specific detection of CIP2A protein or mRNA in bladder tissue as a biomarker for bladder cancer in humans. | 2013-05-09 |
| 20130115600 | SEQUENCES AND THEIR USE FOR DETECTION OF SALMONELLA - This invention relates to a rapid method for detection of | 2013-05-09 |
| 20130115601 | TISSUE TYPING ASSAYS AND KITS - The present invention relates generally to compositions of lyophilised reagents suitable for nucleic acid amplification use in in-vitro diagnostics. More particularly, the invention relates to lyophilised PCR reagent compositions and methods for genotyping including HLA and/or ABO and/or HFE typing. | 2013-05-09 |
| 20130115602 | ENDOGENETIC RETROVIRAL SEQUENCES, ASSOCIATED WITH AUTOIMMUNE DISEASES OR WITH PREGNANCY DISORDERS - A genomic retroviral nucleic material, in an isolated or purified state, at least partially functional or non-functional, wherein the genome comprises a reference nucleotide sequence selected from the group including sequences of SEQ ID NOs: 1-15, their complementary sequences, and their equivalent sequences, in particular, nucleotide sequences having, for every series of 100 contiguous monomers, at least 70% and preferably at least 90% homology with the sequences of SEQ ID NOs: 1-15. | 2013-05-09 |
| 20130115603 | NUCLEOTIDE REPEAT EXPANSION-ASSOCIATED POLYPEPTIDES AND USES THEREOF - Isolated polypeptides that are endogenously expressed from nucleotide repeat expansions are disclosed. In some cases, the polypeptides include polypeptide repeats. In some cases, the polypeptide repeats include at least five contiguous repeats of a single amino acid. In other cases, the repeats include at least six contiguous amino acids of a tetra- or penta-amino acid repeat block. | 2013-05-09 |
| 20130115604 | METHODS AND MATERIALS FOR THE DIAGNOSIS OF PROSTATE CANCERS - Methods for diagnosing the presence of prostate cancer in a subject are provided, such methods including detecting the levels of expression of multiple polypeptide biomarkers in a biological sample obtained from the subject and comparing the levels of expression with predetermined threshold levels. Levels of expression of at least two of the polypeptide markers that are above the predetermined threshold levels are indicative of the presence of prostate cancer in the subject. Determination of the expression levels of specific combinations of biomarkers can also be used to determine the type and/or stage of prostate cancer. | 2013-05-09 |
| 20130115605 | METHOD FOR PREPARATIVE PRODUCTION OF LONG NUCLEIC ACIDS BY PCR - The invention relates to a method for preparative production of long nucleic acids by PCR. The method involves the following hybridization steps: a) a nucleic acid base sequence is hybridized on the 3′ and 5′ ends with an adapter primer; b) the product from step a) is hybridized on the 3′ and 5′ ends with an extension primer containing an extension sequence, wherein a nucleic acid with extension sequences amplified and enlarged in the 3′ and 5′ ends of the nucleic acid base sequence is then formed from the nucleic acid base sequence. The invention also relates to different applications of the inventive method. | 2013-05-09 |
| 20130115606 | SYSTEM AND METHOD FOR MICROFLUIDIC CELL CULTURE - Microfluidic devices and methods for perfusing a cell with perfusion fluid are provided herein, wherein the gravitational forces acting on the cell to keep the cell at or near a retainer or a retaining position exceed the hydrodynamic forces acting on the cell to move it toward an outlet. Also provided, are methods for assaying cell products within the microfluidic device. | 2013-05-09 |
| 20130115607 | Sample Preparation, Processing and Analysis Systems - This disclosure provides an integrated and automated sample-to-answer system that, starting from a sample comprising biological material, generates a genetic profile in less than two hours. In certain embodiments, the biological material is DNA and the genetic profile involves determining alleles at one or a plurality of loci (e.g., genetic loci) of a subject, for example, an STR (short tandem repeat) profile, for example as used in the CODIS system. The system can perform several operations, including (a) extraction and isolation of nucleic acid; (b) amplification of nucleotide sequences at selected loci (e.g., genetic loci); and (c) detection and analysis of amplification product. These operations can be carried out in a system that comprises several integrated modules, including an analyte preparation module; a detection and analysis module and a control module. | 2013-05-09 |
| 20130115608 | SCREENING METHODS FOR OCULAR IRRITATION AND TOXICITY - Methods of determining a level of ocular irritation and/or toxicity for a chemical compound are described. Kits for use in methods of determining a level of ocular irritation and/or toxicity for a chemical compound are also described. | 2013-05-09 |
| 20130115609 | Methods and Kits for Detecting Circulating Cancer Stem Cells - Disclosed herein is the use of LIN28B gene or a variant thereof as a cancer stem cell marker gene for the diagnosis, treatment, or prognosis of a malignant tumor such as hepatocellular carcinoma. Also included herein are methods and kits for detecting circulating cancer stem cells in a subject. According to various embodiments of the disclosure, the methods and kits use the LIN28B gene or a variant thereof as the cancer stem cell marker gene. | 2013-05-09 |
| 20130115610 | METHOD OF IDENTIFYING PREBIOTICS AND COMPOSITIONS CONTAINING THE SAME - A method for identifying test agents that exhibit prebiotic activity on human skin commensal microorganisms and compositions that include such agents. The method includes providing a test culture of a test agent, a human skin commensal microorganism and a minimal carbon medium. The method provides a time efficient and cost effective way to predict in vivo prebiotic activity of a test agent on skin commensal microorganisms. | 2013-05-09 |
| 20130115611 | Systems and Methods for Calibration Using Dye Signal Amplification - The present teachings relate to a method of generating calibration information during a real-time polymerase chain reaction (RT-PCR) or other amplification reaction. A sample well plate or other support can contain one or more dyes or other reference materials that are subjected to the same RT-PCR thermal cycles or other conditions used to conduct amplification or other reactions on a biological sample. A set of maxima values and a set of minimum values, and/or other calibration information useful for adjusting emission data for sample dyes can be recorded, for example, for 10 cycles, 20 cycles, or each cycle of a complete RT-PCR run. Such testing of dye response under realistic operating conditions can enable more accurate characterization of plate, dye, filter, or instrument response and therefore more accurate calibration corrections and other and/or adjustments. | 2013-05-09 |
| 20130115612 | METHOD FOR ANALYZING MUCIN 1 HAVING SIAALPHA2-8SIAALPHA2-3GALBETA GLYCANS - The object of the present invention is to provide a clinical marker capable of distinguishing breast cancer from interstitial pneumonia; and a clinical marker for detecting malignancy or progress level of breast cancer, and for monitoring effects of the treatment of breast cancer. The object can be solved by a method for analyzing mucin 1 having Siaα2-8Siaα2-3Galβ-R, characterized by comprising the step of bringing a first probe specifically binding to a mucin 1 having Siaα2-8Siaα2-3Galβ-R into contact with a sample to be tested. | 2013-05-09 |
| 20130115613 | SCREENING ASSAYS BASED ON MAG AND/OR ABHD6 FOR SELECTING INSULIN SECRETION PROMOTING AGENT - The present application relates to a method of characterizing an agent's ability to increase insulin secretion in a subject. The method comprises determining whether the agent is able to modulate MAG level at the inner surface of the cytoplasmic membrane of a cell and/or ABHD6 activity. The agent is characterized as having the ability to increase insulin secretion in the subject when it is capable of upregulating MAG level at the inner surface of the cytoplasmic membrane and/or downregulating ABHD6 activity. | 2013-05-09 |
| 20130115614 | METHOD FOR DETECTING AND QUANTIFYING ENDOGENOUS WHEAT DNA SEQUENCE - A primer pair is provided capable of amplifying partial sequences of endogenous wheat DNA which are single copies and which allow wheat to be specifically detected without cross-reacting with other plants in a polymerase chain reaction. Also provided is a kit for detecting or assaying an endogenous wheat DNA sequence in a test sample by a polymerase chain reaction. | 2013-05-09 |
| 20130115615 | METHOD OF CLASSIFYING HUMAN SUBJECTS HAVING ADOLESCENT IDIOPATHIC SCOLIOSIS (AIS) AND METHOD FOR SCREENING FOR A COMPOUND USEFUL IN THE TREATMENT OF AIS AND RELATED SYNDROMES CAUSING SPINAL DEFORMITIES - A method of classifying a human subject having adolescent idiopathic scoliosis (AIS) comprising: providing a cell sample isolated from the subject; detecting an impairment in melatonin-signaling pathway in the sample in the presence and in the absence of a known melatonin-signaling pathway agonist, whereby the results of the detecting step enables the classification of the subject having AIS in one AIS subgroup; and a method of screening for a compound useful in the treatment of a disease characterized by a dysfunctional melatonin-signaling pathway, said method comprising the steps of contacting a candidate compound with at least one cell expressing at least one melatonin-signaling pathway impairment, wherein the candidate compound is selected if said melatonin-signaling pathway impairment is reduced in the presence of the candidate compound as compared to that in the absence thereof. | 2013-05-09 |
| 20130115616 | DETECTION OF NUCLEIC ACIDS BY AGGLUTINATION - Embodiments of the invention relate generally to methods detecting, quantifying, or purifying nucleic acids by way of agglutination reactions. Several embodiments amplify target nucleic acids while incorporating a label such as 5-methyl-cytosine into amplified product and detecting, quantifying, or purifying the product with latex beads coupled to antibody reactive to the 5-methyl-cytosine labeled nucleic acid. Several embodiments incorporate DNA labels into specifically designed primers in order to detect, quantify, or purify a product after agglutination. | 2013-05-09 |
| 20130115617 | METHODS OF CELL CULTURE FOR ADOPTIVE CELL THERAPY - Production and use of novel therapeutic cells, called T-Vehicles, in the allogeneic Adoptive Cell Therapy setting allows a wide range of therapeutic benefits to accrue with minimal or no risk of GVHD. T-Vehicles are created from donor T cells that are altered to contain therapeutic attributes that do not include their native antigen receptors and can deliver therapeutic benefits irrelevant of their native antigen specificity. T-Vehicles can possess highly restricted native antigen specificity that renders them unable to recognize antigens present on normal cells and incapable of initiating GVHD, making them ideal transport vehicles to deliver various therapeutic attributes in vivo. In essence, production and use of T-Vehicles is a paradigm shift that opens the door to therapeutic application of T cells in ways not previously contemplated, independent of whether or not there is an HLA match between the donor and the recipient. | 2013-05-09 |
| 20130115618 | PHOSPHOLIPID PROFILING AND CANCER - In general, the present invention provides prognostic and predictive methods and kits for determining the lipogenicity of a tumor in a subject, by making use of phospholipid profiling, whereby a relative increase in mono-unsaturated phospholipid species in combination with a relative decrease in poly-unsaturated phospholipid species is indicative for a more resistant and aggressive lipogenic cancer phenotype. | 2013-05-09 |
| 20130115619 | UBIQUITINATION ASSAY - The present invention related to a method of assaying ubiquitination in a sample by combining ubiquitin and two or more of E1, E2, E3 and a substrate protein in a sample under conditions suitable for ubiquitination to take place, exposing the sample with a labelled binding partner which is specific for the ubiquitin and measuring the amount of labelled ubiquitin bound to any one of the components in the sample, wherein one or more of the components in the sample comprises an immobilisation tag which facilitates its immobilisation onto a solid surface. | 2013-05-09 |
| 20130115620 | Novel Hemopoietin Receptor Protein, NR10 - The inventors succeeded in isolating a novel hemopoietin receptor gene (NR10) using a sequence predicted from the extracted motif conserved in the amino acid sequences of known hemopoietin receptors. It was expected that two forms of NR10 exists, a transmembrane type and soluble form. Expression of the former type was detected in tissues containing hematopoietic cells. Thus, NR10 is a novel hemopoietin receptor molecule implicated in the regulation of the immune system and hematopoiesis in vivo. These novel receptors are useful in screening for novel hematopoietic factors capable of functionally binding to the receptor, or developing medicines to treat diseases related with the immune system or hematopoietic system. | 2013-05-09 |
| 20130115621 | HIGHLY SENSITIVE IMMUNOASSAYS AND ANTIBODIES FOR DETECTION OF BLOOD FACTOR VIII - Disclosed are antibodies that selectively bind to blood coagulation factor FVIII, and highly sensitive immunological assays comprising these antibodies. Preferred assays can detect FVIII at about 3500-fold below the normal physiological levels, and have a wide array of applications including accurate monitoring of FVIII concentration in pharmaceutical products for treatment of blood coagulation disorders, and determination of FVIII levels in plasma of human patients, including those with blood coagulation disorders such as hemophilia. | 2013-05-09 |
| 20130115622 | PHARMACEUTICAL COMPOSITION FOR PREVENTION AND TREATMENT OF AMYOTROPHIC LATERAL SCLEROSIS - Provided are a prophylactic and therapeutic agent for amyotrophic lateral sclerosis containing an HMG-CoA reductase inhibitor and a method of screening for a prophylactic and therapeutic drug for amyotrophic lateral sclerosis using an induced pluripotent stem cell derived from a patient with amyotrophic lateral sclerosis. | 2013-05-09 |
| 20130115623 | USE OF MARKERS OF UNDIFFERENTIATED PLURIPOTENT STEM CELLS - The disclosure relates to methods of binding and identifying undifferentiated pluripotent stem cells and particularly, although not exclusively, to use of binding moieties which bind to PHB on the surface of undifferentiated pluripotent stem cells, such as PHB-binding peptides, and to methods for depleting undifferentiated stem cells from a sample. | 2013-05-09 |
| 20130115624 | Methods To Identify Modulators - Disclosed are compounds that activate the human G-protein coupled receptor TAS2R41, and methods of using these compounds for identifying compounds that modulate the response of the TAS2R41 receptor. Compounds identified as modulators of the response of the receptor TAS2R41 may be used to decrease or mask the bitter taste of foods or drugs. | 2013-05-09 |
| 20130115625 | NOVEL PROTEIN AND USE THEREOF - A protein being the following (A), (B), or (C): (A) a protein represented by the amino acid sequence of SEQ ID NO: 1; (B) a protein represented by an amino acid sequence in which one or a plurality of amino acid is substituted, deleted, inserted or added in the amino acid sequence of SEQ ID NO: 1, the protein having binding activity specific for a mixture of a sphingolipid and cholesterol; or (C) a protein represented by an amino acid sequence being at least 70% identical to the amino acid sequence of SEQ ID NO: 1. | 2013-05-09 |
| 20130115626 | ANTI-NEUROPILIN ANTIBODIES AND METHODS OF USE - The invention provides anti-NRP1 antibodies and methods of using the same. | 2013-05-09 |
| 20130115627 | METHODS FOR IDENTIFYING CANDIDATE CYTOTOXIC ANTIBODY MOLECULES - The disclosure relates to methods for screening candidate antibody molecules which bind to podocalyxin-like protein (PODXL) and/or to undifferentiated pluripotent stem cells and particularly, although not exclusively, to methods for identifying candidate cytotoxic antibody molecules. | 2013-05-09 |
| 20130115628 | METHOD FOR SELECTION OF CHEMOTHERAPEUTIC AGENTS FOR ADENOCARCINOMA CANCER - The subject invention relates to determining the presence and level of hENT1 expression in tumor tissue that is appropriate for gemcitabine therapy, and more importantly, the level of hENT1 expression that signifies that treatment with a gemcitabine derivative is a more appropriate strategy. | 2013-05-09 |
| 20130115629 | METHODS OF BREAST CANCER DETECTION - Methods of diagnosing breast cancers are provided. The methods include analyzing the expression patterns of biomarkers in a plurality of cells to distinguish between invasive and non-invasive cancers, usual and atypical hyperplasias, and basal-like breast cancers. Breast cancers are diagnosed based upon different combinations of the biomarkers found in nipple aspirate fluid. | 2013-05-09 |
| 20130115630 | AUTOMATED MULTIPLEX IMMUNOASSAY - Embodiments relate generally to an automated multiplex immunoassay for qualitatively and/or quantitatively detecting a plurality of targets on a tissue sample. Kits and apparatuses for practicing such assays are also provided. | 2013-05-09 |
| 20130115631 | Methods For Measuring High Molecular Weight Complexes Of Fibrinogen With Fibronectin And Fibulin-1 - A method of detecting MSDX Complex-1, the method introducing a first antibody to a sample to create an antibody-sample mixture, wherein the first antibody is specific for one of fibrinogen, fibronectin, or fibulin-1, the first antibody having a label molecule; providing a well coated with a second antibody, the second antibody is specific for one of fibrinogen, fibronectin, or fibulin-1; introducing the antibody-sample mixture to the well; and introducing a substrate to the antibody-sample mixture in the well, wherein the label molecule and the substrate interact to provide a signal, wherein when the signal is detected then MSDX Complex-1 is detected. | 2013-05-09 |
| 20130115632 | HIGHLY SENSITIVE METHOD FOR ASSAYING TROPONIN I - A highly sensitive method of assaying an analyte present in a sample using protamine is provided, as well as an immunoassay reagent and biosensor that reduces non-specific binding by electrostatic interaction of protamine with fibrinogen present in the sample. | 2013-05-09 |
| 20130115633 | Methods & Devices for Diagnosing Cardiac Disorders - A method for diagnosing a cardiac disorder by detecting levels of cardiac-specific membrane polypeptides in tissue samples. | 2013-05-09 |
| 20130115634 | PEPTIDES AND METHODS FOR THE DETECTION OF LYME DISEASE ANTIBODIES - The invention provides compositions (e.g., peptide compositions) useful for the detection of antibodies that bind to | 2013-05-09 |
| 20130115635 | EPITOPE TAG FOR AFFINITY-BASED APPLICATIONS - Described is an epitope tag useful in affinity-based applications. The invention further includes fusion proteins, methods for preparing fusion proteins, nucleic acid molecules encoding these fusion proteins and recombinant host cells that contain these nucleic acid molecules. The invention also relates to nanobodies and other affinity ligands specifically recognizing the epitope tag, and uses thereof in affinity-based applications. | 2013-05-09 |
| 20130115636 | HISTONE CITRULLINATED PEPTIDES AND USES THEREOF - The present invention refers to citrullinated synthetic peptides derived from the histone H4 protein and their use in the diagnosis of autoimmune diseases,particularly Rheumatoid Arthritis (RA). | 2013-05-09 |
| 20130115637 | Efficiency of Prion Conversion in vitro and Sensitivity of Prion Detection - The present invention relates to improved methods and kits for the amplification detection of pathogenic prion proteins in samples. In some aspects of the invention, the method comprises: i) contacting the sample with a source of PrP | 2013-05-09 |
| 20130115639 | METHOD FOR DETECTING A SALMONELLA INFECTION - The invention relates to a diagnostic method to detect a salmonellae infection and/or salmonellae contamination. Fields of use include medicine, veterinary medicine and various branches of industry. | 2013-05-09 |
| 20130115640 | ACTH for Treatment of Kidney Disease - Provided herein are methods for prophylactic treatment of Diabetes Mellitus comprising administration of adrenocorticotropic hormone (ACTH), or fragment, analog, complex or aggregate thereof, or any combination thereof, to an individual suspected of having, predisposed to, or at risk of developing Diabetes Mellitus. Also provided herein are methods for monitoring treatment of Diabetes Mellitus by measuring and monitoring levels of MCP-1, TGF-β, VEGF A and VEGF B. | 2013-05-09 |
| 20130115641 | ISOLATED LUCIFERASES AND THE USE THEREOF - The invention relates to the nucleotide and amino acid sequences, and to the activity and use, of the luciferases LuAL, Lu164, Lu16, Lu39, Lu45, Lu52 and Lu22. | 2013-05-09 |
| 20130115642 | METHOD FOR SCREENING DIABETES TREATING AGENT - The present invention provides a method for screening a diabetes treating agent comprising the following steps: (a) preparing cells inside a culture medium; (b) treating the culture medium with a test substance; (c) treating the result of step (b) with a glucose derivative which is not metabolized; and (d) measuring the glucose derivative which has flowed into the cells, wherein the test substance is determined to be a diabetes treating agent if the test substance accelerates uptake of the glucose derivative in the cells. Therefore, the described pharmaceutical composition, including a triazin-based compound as an active ingredient, can be useful for application in preventing or treating diabetes. | 2013-05-09 |
| 20130115643 | Colorimetric Device for Detecting, in an Aqueous Solution of Interest, Hydrolytic Enzymatic Activity with Regard to at Least One Polymer of Interest - This invention concerns a colorimetric device for detecting, in an aqueous solution of interest, a hydrolytic enzymatic activity with regard to at least one polymer of interest. | 2013-05-09 |
| 20130115644 | MASS SPECTROMETRY METHOD FOR MEASURING THIAMINE IN BODY FLUID - Provided are methods for determining the amount of total thiamine in a body fluid sample using liquid chromatography and mass spectrometry. Total thiamine is converted to free thiamine by treatment with an acid phosphatase prior to thiamine separation and quantification. | 2013-05-09 |
| 20130115645 | FGL-2 PROTHROMBINASE AS A DIAGNOSTIC TOOL FOR MALIGNANCY - The present: invention reveals a strong correlation between FGL-2 prothrombinase activity levels and the presence of a malignant proliferative disorder in a subject. Thus, the present invention provides FGL-2 prothrombinase activity as a diagnostic tool for malignancy. | 2013-05-09 |
| 20130115646 | METHOD FOR MEASURING GLYCATED HEMOGLOBIN - It is to provide a method for measuring glycated hemoglobin in a hemoglobin-containing sample, comprising: reacting glycated hemoglobin in the hemoglobin-containing sample with a proteolytic enzyme in the presence of at least one salt selected from the group consisting of a pyridinium salt, a phosphonium salt, an imidazolium salt, and an isoquinolinium salt; reacting the obtained reaction product with fructosyl peptide oxidase; and measuring the generated hydrogen peroxide. The present invention provides a method for accurately measuring glycated hemoglobin in a hemoglobin-containing sample. | 2013-05-09 |
| 20130115647 | Method to Increase the Number of Detectable Photons During the Imaging of a Biological Marker - The present invention relates a method to determine the presence of a photon producing biological marker in a cell, tissue or organism of interest. The method is based on Fluorescence by Unbound Excitation from Luminescence (FUEL) and comprises the steps of a) providing conditions suitable for the biological marker to produce at least one first photon by luminescence; b) providing a FUEL probe pair-upper (FPP-U) disposed in proximity to the cell, tissue or organism, wherein the at least one first photon of step a) excites the FPP-U, which emits at least one second photon. The FPP-U may be selected from the group of quantum dots, carbon nanotubes, fluorescent proteins, diamond nanocrystals and metalloporphyrins. This method is charcterized in that said biological marker and said FPP-U are not bound and in that each of the at least one second photon(s) are of a longer wavelength than each of the at least one first photon(s). | 2013-05-09 |
| 20130115648 | METHOD OF IDENTIFYING PREBIOTICS AND COMPOSITIONS CONTAINING THE SAME - A high-throughput, tiered screening method of indentifying test agents that exhibit prebiotic activity on human skin commensal microorganisms and cosmetic compositions that include such agents. The method includes determining the metabolite level of a culture and comparing the metabolite level to a control value. The assay further comprises performing a plate count using the culture when the metabolite level of the culture is greater than its corresponding control value. The plate count results are compared to another control value, and the test agent is identified as a prebiotic agent when the number of colonies present in the plate count assay of the culture is greater than the second control value. | 2013-05-09 |
| 20130115649 | Methods and Reagents for Metabolomics and Histology in a Biological Sample and a Kit for the Same - A method of extracting and measuring one or more biochemicals from a biological sample, comprises immersing the biological sample in an organic solvent, whereby one or more biochemicals present in the biological sample are extracted into the organic solvent; separating the biological sample from the free organic solvent; and measuring the level(s) of the one or more biochemicals extracted into the organic solvent, wherein the biological sample remains analyzable by histological analysis. | 2013-05-09 |
| 20130115650 | Isotopic Biomarkers for Rapid Assessment of Bone Mineral Balance in Biomedical Applications - The present invention relates to the use of natural isotopes | 2013-05-09 |
| 20130115651 | FLUORESCENT DYES - The present invention provides dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes provided in this invention can comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been substituted with specific groups to provide beneficial properties. | 2013-05-09 |
| 20130115652 | TISSUE ARRAY PRODUCTION METHOD - A tissue array production method which enables even roll-shaped tissue pieces having various diameters to be steadily fixed to a substrate block is provided. | 2013-05-09 |
| 20130115653 | METHOD FOR PRODUCING BIOBASED CHEMICALS FROM WOODY BIOMASS - A method for utilizing woody biomass components, namely cellulose, hemicellose, and lignin, and converting them to value-added biobased chemical products is described herein. The present method provides treatments to obtain a plurality of component streams from woody biomass for producing derivative products while minimizing waste products. | 2013-05-09 |
| 20130115654 | METHOD FOR PRODUCING BIOBASED CHEMICALS FROM AGRICULTURAL BIOMASS - A method for utilizing agricultural biomass components, namely cellulose, hemicellose, and lignin, and converting them to value-added biobased chemical products is described herein. The present method provides treatments to obtain a plurality of component streams from agricultural biomass for producing derivative products while minimizing waste products. | 2013-05-09 |
| 20130115655 | METHOD FOR THE PRODUCTION OF A LYSATE USED FOR CELL-FREE PROTEIN BIOSYNTHESES - The invention relates to a method for producing a lysate used for cell-fee protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-fee protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also discussed are said lysate and the use thereof. | 2013-05-09 |
| 20130115656 | ENGINEERED YEAST CELLS AND USES THEREOF - The present application provides engineered yeast cells and uses thereof. In specific embodiments, the yeast cells have a mutation in the GAL2 gene. In specific embodiments, the yeast cells can be used for producing a protein or compound of interest. | 2013-05-09 |
| 20130115657 | HUMANIZED ANTI-CD19 ANTIBODIES AND THEIR USE IN TREATMENT OF ONCOLOGY, TRANSPLANTATION AND AUTOIMMUNE DISEASE - The present invention provides chimeric and humanized versions of anti-CD19 mouse monoclonal antibodies. The invention further relates to pharmaceutical compositions, immunotherapeutic compositions, and methods using therapeutic antibodies that bind to the human CD19 antigen and that may mediate ADCC, CDC, and/or apoptosis for the treatment of B cell diseases and disorders, such as, but not limited to, B cell malignancies, for the treatment and prevention of autoimmune disease, and for the treatment and prevention of graft-versus-host disease (GVHD), humoral rejection, and post-transplantation lymphoproliferative disorder in human transplant recipients. | 2013-05-09 |
| 20130115658 | REVERSIBLE, PARALLEL AND MULTITASK CLONING METHOD AND KIT - The present invention is related to integrated method and tools to construct recombinant DNA molecules (to be used as DNA vaccine or gene therapy) without requiring the use of antibiotic(s) resistance gene(s) and without requiring the addition of one or more antibiotic(s) to the culture medium of cells submitted to this recombinant DNA method. The present invention allows to obtain the selection of recombinant host cell(s) transformed by a (exogenous) nucleic acid sequence of interest (extra-chromosomal vector containing the insert) and simultaneously stabilization (stable inheritance) of this (exogenous) nucleic acid sequence of interest into the transformed host cell(s) descendants (maintenance of the nucleic acid sequence of interest in the host cells population). | 2013-05-09 |
| 20130115659 | COMPOSITIONS AND METHODS FOR GRAIN PROCESSING WITHOUT PH ADJUSTMENT - Described are compositions and methods relating to starch processing without a phytase pretreatment step and without adjustment of the slurry pH adjustment. | 2013-05-09 |
| 20130115660 | METHOD OF PRODUCING SUGAR - Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention. | 2013-05-09 |
| 20130115661 | METHOD FOR PRODUCING BIOBASED CHEMICALS FROM CULTIVATED PLANT BIOMASS - A method for utilizing cultivated plant biomass components, namely cellulose, hemicellose, and lignin, and converting them to value-added biobased chemical products is described herein. The present method provides treatments to obtain a plurality of component streams from cultivated plant biomass for producing derivative products while minimizing waste products. | 2013-05-09 |
| 20130115662 | FUNGAL PEROXYGENASES AND METHODS OF APPLICATION - The invention relates to polypeptides having peroxygenase activity and compositions comprising such polypeptides, their encoding polynucleotides, expression vectors and recombinant host cells comprising such polynucleotides or vectors, methods of producing the polypeptides, as well as methods of application and uses thereof, including a process for enzymatic, regioselective oxygenation of N-heterocycles of the general formula (I) to the corresponding N-oxides of the formula (II), by converting N-heterocycles of the formula (I) with a peroxidase polypeptide in the presence of at least one oxidizing agent in a one-stage reaction process. | 2013-05-09 |
| 20130115663 | ENONE REDUCTASES - The disclosure relates to engineered enone reductase polypeptides having improved properties, polynucleotides encoding the engineered polypeptides, related vectors, host cells, and methods for making the engineered enone reductase polypeptides. The disclosure also provides methods of using the engineered enone reductase polypeptides for chemical transformations. | 2013-05-09 |
| 20130115664 | PROCESS AND SYSTEM FOR PRODUCING ALGAL OIL - A method for producing an algal oil is provided. The method includes continuously providing a growth medium and an algal strain to a bioreactor at a predetermined fluid flow rate; illuminating the growth medium and algal strain contained within the bioreactor by a first artificial light source for a time sufficient to effect lipid production by the algal strain; continuously withdrawing a portion of the growth medium and algal strain contained within the bioreactor at the predetermined fluid flow rate; and treating the withdrawn portion of the growth medium and algal strain to produce and isolate a lipid produced by the algal strain. | 2013-05-09 |
| 20130115665 | METHODS AND COMPOSITIONS FOR PRODUCING FATTY ALCOHOLS - Methods and compositions, including nucleotide sequences, amino acid sequences, and host cells, for producing fatty alcohols are described. | 2013-05-09 |
| 20130115666 | METHOD FOR PRODUCTION OF EUGLENA CONTAINING WAX ESTER AT HIGH COTENT, AND METHOD FOR PRODUCTION OF WAX ESTER - Provided is a method for producing a | 2013-05-09 |
| 20130115667 | Amycolatopsis sp. Strain and Methods of Using the Same for Vanillin Production - This invention provides an | 2013-05-09 |
| 20130115668 | HOST CELLS AND METHODS FOR PRODUCING FATTY ACID DERIVED COMPOUNDS - The present invention provides for a method of producing one or more fatty acid derived compounds in a genetically modified host cell which does not naturally produce the one or more derived fatty acid derived compounds. The invention provides for the biosynthesis of fatty acid derived compounds such as C18 aldehydes, C18 alcohols, C18 alkanes, and C17 alkanes from C18-CoA which in turn is synthesized from butyryl-CoA. The host cell can be further modified to increase fatty acid production or export of the desired fatty acid derived compound, and/or decrease fatty acid storage or metabolism. | 2013-05-09 |
| 20130115669 | BIOPROCESSING LIGNO-CELLULOSE INTO ETHANOL WITH RECOMBINANT CLOSTRIDIUM - The present invention relates, inter alia, to recombinant Gram-positive | 2013-05-09 |
| 20130115670 | CELL DISRUPTION - A process for the disruption of a biological cell comprising freezing, boiling or perhaps alternately freezing and boiling the material containing the biological cell using a thermoelectric cell a base face whereof is contiguous with a heat sink/source held at a substantially constant temperature and a working face . Apparatus for carrying out the disruption process comprises a peltier cell a base face of which is flexibly attached to a heat sink arranged to be kept at a constant temperature of around 50° C. and a working face of which is contiguous with a reaction vessel or a reaction vessel holder. Reversal of the voltage in the peltier cell enables the working face alternately to reach below freezing and above boiling temperatures, and/or with use of a resistive wire on the vessel holder for heating with the TEC used purely for cooling The peltier cell base face is constructed of materials which tend to inhibit disintegration of the peltier cell brought about by expansion and contraction under heat. | 2013-05-09 |
| 20130115671 | ENZYMES FROM CONIDIOBOLUS BREFELDIANUS AND PROCESS FOR PREPARATION THEREOF - The instant invention relates to a fungus of the | 2013-05-09 |
| 20130115672 | BIOLOGICAL COMPOSITE AND METHOD FOR REDUCING H2S - A biological composite and method for reducing H | 2013-05-09 |
| 20130115673 | Methods of Screening Embryonic Progenitor Cell Lines - Aspects of the present invention include methods and compositions related to the production and use of numerous clonal lineages of embryonic progenitor cell lines derived from differentiating cultures of primordial stem cells with diverse molecular markers and having been cultured for >21 doublings of clonal expansion. The robustness of these clonally-purified lines, their ability to expand for >40 passages while maintaining their pattern of gene expression, lack of tumorigenicity, and their embryonic pattern of gene expression offers novel compositions and methods for modeling numerous differentiation pathways for the first time in vitro, and for the manufacture of purified product not existing in such a purified state in nature or using other manufacturing modalities. Representative progenitor cell lines described herein are capable of development into cutaneous adipocytes, blood-brain barrier cells, neuronal cells, or smooth muscle cells each with therapeutic potential. | 2013-05-09 |
| 20130115674 | Human Monoclonal Antibodies to Human Nucleolin - The present invention provides for methods of producing human monoclonal antibodies to human nucleolin, cells producing such antibodies, and the antibodies themselves. Also provided are methods of using the antibodies in diagnosing and treating malignant and non-malignant diseases wherein cells that express nucleolin on the cell surface contribute to the pathophysi-ology of the disease. | 2013-05-09 |
| 20130115675 | Dehalogenases, Nucleic Acids Encoding Them and Methods for Making and Using Them - The invention relates to haloalkane dehalogenases and to polynucleotides encoding the haloalkane dehalogenases. In addition methods of designing new dehalogenases and method of use thereof are also provided. The dehalogenases have increased activity and stability at increased pH and temperature. | 2013-05-09 |
| 20130115676 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 2013-05-09 |
| 20130115677 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 2013-05-09 |
| 20130115678 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 2013-05-09 |
| 20130115679 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 2013-05-09 |
| 20130115680 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 2013-05-09 |
| 20130115681 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 2013-05-09 |
| 20130115682 | FUSION PROTEINS - The invention provides a single chain, polypeptide fusion protein, comprising: a non-cytotoxic protease, or a fragment thereof, which protease or protease fragment is capable of cleaving a protein of the exocytic fusion apparatus of a target cell; a Targeting Moiety that is capable of binding to a Binding Site on the target cell, which Binding Site is capable of undergoing endocytosis to be incorporated into an endocome within the target cell; a protease cleaving site at which site the fusion protein is cleavable by the protease, wherein the protease cleavage site is located between the non-cytotoxic protease or fragment thereof and the Targeting Moiety; and the translocation domain that is capable of translocating the protease or protease fragment from within an endosome, across the endosomal membrane and into the cytosol of the target cell. | 2013-05-09 |
| 20130115683 | METHOD FOR THE PREPARATION OF A PHARMACEUTICAL COMPOSITION - The present invention relates to a method for the preparation of a pharmaceutical composition for the prevention or/and treatment of an influenza virus infection. | 2013-05-09 |
| 20130115684 | METHODS AND SYSTEMS FOR REDUCTION OF HALOGENATED COMPOUNDS - Methods and systems for dehalogenating organohalides are disclosed. In one respect, the systems can generate hydrogen in an electrolysis cell and supply the hydrogen to anaerobic dehalogenating bacteria to decontaminate organohalides at a contamination site. | 2013-05-09 |
| 20130115685 | POINT-OF-CARE FLUIDIC SYSTEMS AND USES THEREOF - This invention is in the field of medical devices. Specifically, the present invention provides portable medical devices that allow real-time detection of analytes from a biological fluid. The methods and devices are particularly useful for providing point-of-care testing for a variety of medical applications. | 2013-05-09 |
| 20130115686 | METHOD OF MANUFACTURING MICRO CHAMBER PLATE WITH BUILT-IN SAMPLE AND ANALYTIC MICRO CHAMBER PLATE, ANALYTIC MICRO CHAMBER PLATE AND APPARATUS SET FOR MANUFACTURING ANALYTIC MICRO CHAMBER PLATE WITH BUILT-IN SAMPLE - The present invention relates to a micro chamber plate, and more particularly, to an analytic micro chamber plate in which a plurality of reaction solutions including a primer or probe selectively reacting with a nucleic acid react with each other without cross-contamination to measure and analyze a fluorescence level in real-time so as to analyze biological sample solution including a large amount of nucleic acids. Also, the present invention relates to a method of manufacturing the analytic chamber plate. Also, the present invention relates to a method of manufacturing a micro chamber plate with a built-in sample used for manufacturing the analytic chamber plate. Also, the present invention relates to an apparatus set for manufacturing the micro chamber plate with a built-in sample. | 2013-05-09 |
| 20130115687 | POST PROTEIN HYDROLYSIS REMOVAL OF A POTENT RIBONUCLEASE INHIBITOR AND THE ENZYMATIC CAPTURE OF DNA - The present invention concerns compositions and methods of extracting infectious pathogens from a volume of blood. In one embodiment, the method includes the steps of creating a fibrin aggregate confining the pathogens and introducing a fibrin lysis reagent to expose the pathogens for analysis. The present invention also concerns materials and methods for removing aurintricarboxylic acid (ATA) from a sample. | 2013-05-09 |
| 20130115688 | LAMINAR PHOTOBIOREACTOR FOR THE PRODUCTION OF MICROALGAE - A modular photobioreactor for the production of microalgae particularly suited for absorber emission gases with a high carbon dioxide (CO | 2013-05-09 |
| 20130115689 | PHOTOBIOREACTOR - Provided herein is a transgenic bacteria engineered to accumulate carbohydrates, for example disaccharides. Also provided is a photobioreactor for cultivating photosynthetic microorganisms comprising a non-gelatinous, solid cultivation support suitable for providing nutrients and moisture to photosynthetic microorganisms and a physical barrier covering at least a portion of the surface of the cultivation support. Devices for the large scale and continuous cultivation of photosynthetic microorganisms incorporating photobioreactors and methods of use are disclosed. Also disclosed are methods of producing fermentable sugar from photosynthetic microorganisms using a photobioreactor of the invention. | 2013-05-09 |
| 20130115690 | Cell Culture Vessel and Cell Culture Device - Disclosed is a cell culture vessel that can prevent liquid leakage from the cell culture space and entry of particles containing microorganisms from outside the cell culture space, and that can prepare regenerated tissue in a manner that is safe and results in peace of mind. The cell culture vessel is mounted to a cell culture device, holds/cultures cells, and is characterized by being provided with: a culture solution holding section that holds a culture solution, a protruding structure section that is for supplying and discharging the aforementioned culture solution; and a culture solution duct that passes from the aforementioned protruding structure section to the aforementioned culture solution holding section. | 2013-05-09 |
| 20130115691 | THERMO-CONDUCTIVE CELL CULTURE DISH HOLDER - The present invention describes various devices for holding cell culture dishes in a secure manner and to ensure rapid and uniform heat transfer to and from the cell culture dish. | 2013-05-09 |
| 20130115692 | METHODS AND COMPOSITIONS RELATING TO IMPROVED LENTIVIRAL VECTORS AND THEIR APPLICATIONS - The present invention provides HIV-derived lentivectors which are safe, highly efficient, and very potent for expressing transgenes for human gene therapy, especially, in human hematopoietic progenitor cells as well as in all other blood cell derivatives. The lentiviral vectors comprise a self-inactivating configuration for biosafety and promoters such as the EF1α promoter as one example. Additional promoters are also described. The vectors can also comprise additional transcription enhancing elements such as the wood chuck hepatitis virus post-transcriptional regulatory element. These vectors therefore provide useful tools for genetic treatments such as inherited and acquired lympho-hematological disorders, gene-therapies for cancers especially the hematological cancers, as well as for the study of hematopoiesis via lentivector-mediated modification of human HSCs. | 2013-05-09 |
