| 18th week of 2012 patent applcation highlights part 53 |
| Patent application number | Title | Published |
|---|
| 20120107816 | Primer Set for Detecting EGFR Exon 21 Polymorphism and Application Thereof - The invention provides a primer set for detecting a polymorphism in EGFR exon 21 L858R. The primer set has a P1 oligonucleotide and a P2 oligonucleotide and can performing amplification by using a region including the 172792nd base of SEQ ID NO: 1 as a template. As a base that is complementary to the 172792nd base of SEQ ID NO: 1, the P1 oligonucleotide has cytosine and the P2 oligonucleotide has adenine. The melting temperature of the P1 oligonucleotide is higher than the melting temperature of the P2 oligonucleotide, and/or the P1 oligonucleotide is one or more bases longer than the P2 oligonucleotide. The invention further provides a polymorphism detection primer, a polymorphism detection method using the primer set, a method of evaluating a EGFR tyrosine kinase inhibitor using the primer set, a primer used in the polymorphism detection method, and a kit including the primer set. | 2012-05-03 |
| 20120107817 | Probe for Detection of Polymorphism in EGFR Gene, Amplification Primer, and Use Thereof - A polymorphism-detecting probe, an amplification primer and the use thereof are provided to enable simple and highly reliable determination of different polymorphisms in an EGFR gene. | 2012-05-03 |
| 20120107818 | DETECTION OF MULTIPLE NUCLEIC ACID SEQUENCES IN A REACTION CARTRIDGE - The present invention relates to a method for amplifying and detecting nucleic acid sequences in a reaction cartridge comprising the following steps, (i) providing a sample comprising at least one nucleic acid molecule, (ii) in a first reaction chamber of the cartridge providing reagents for an amplification reaction, (iii) mixing the sample with the amplification reagents, (iv) amplifying the at least one nucleic acid in the first reaction chamber of the cartridge, (v) transferring at least parts of the amplification reaction into a second and third reaction chamber of the cartridge each comprising a probe set, wherein (a) each probe set consists of at least three probes, (b) each of the probes is specific for a nucleic acid sequence, (c) there are at least two probes in each set which carry an identical label, (d) each of the probes in a given probe set that carries an identical label has a melting temperature (T | 2012-05-03 |
| 20120107819 | Detection of Clostridium difficile - The invention provides methods to detect | 2012-05-03 |
| 20120107820 | Multiplexed Quantitative PCR End Point Analysis of Nucleic Acid Targets - Certain embodiments of the present invention are directed to one pot multiplexed quantitative PCR methods for end point analysis of a plurality of nucleic acid targets in a complex sample without user intervention, and to various encoded particles on which are immobilized one or more probes that hybridize with the plurality of targets. Certain other embodiments are directed to a new “multiple-color genetic variation detection method” that can detect SNPs and kit using one chamber multiplexed endpoint PCR and differentially labeled allele-specific primers (one recognizing only the wild type allele and one only the mutant allele). | 2012-05-03 |
| 20120107821 | METHODS FOR THE DIAGNOSIS AND PROGNOSIS OF LEUKEMIA AND OTHER CANCER TYPES - Methods for diagnosing leukemia and other cancer types, so that the malignancy thereof dependent on expression of ARTS at low levels or an absence of ARTS expression are disclosed. Moreover methods for prognosis of leukemia and other cancer types, which are prone for an effective treatment by restoring ARTS expression levels and/or restoring cellular ARTS activity are further disclosed. | 2012-05-03 |
| 20120107822 | METHOD OF DELIVERING PCR SOLUTION TO MICROFLUIDIC PCR CHAMBER - The present invention relates to systems and methods of performing in-line mixing of assay components and delivery of such mixed components into microfluidic channels. In one aspect, a method of delivering mixed assay components is provided which comprises causing an unmixed primer solution to flow into a first mixing channel, the unmixed primer solution comprising a common reagent and a primer, holding the unmixed primer solution in the first mixing channel for at least a threshold amount of time to allow the unmixed primer solution to transition into a mixed primer solution, causing a buffer to flow into a second mixing channel, the buffer comprising the common reagent but not including a primer, after holding the unmixed primer solution in the first mixing channel for at least the threshold amount of time, drawing, from the first mixing channel, the mixed primer solution into a common exit channel, and after drawing the mixed primer solution into the exit channel, drawing, from the second mixing channel, the buffer into the common exit channel. | 2012-05-03 |
| 20120107823 | MICRO-DEVICE AND METHODS FOR DISRUPTING CELLS - A micro-device for disrupting cells includes a first chamber in which the cells are disrupted, a second chamber which is pressurized and depressurized, a flexible membrane which separates the first chamber and the second chamber and is vibrated by pressuring and depressurizing the second chamber, and a micro-unit confined in the first chamber, where the micro-unit disrupts the cells in the first chamber | 2012-05-03 |
| 20120107824 | mPCR Methods for Analyzing Repeat Sequences - Methods are provided for determining the methylation status of GC-rich templates. The methods include use of GC reference standards that allow simultaneous characterization of methylation status and CGG repeat length. The methods are useful for detecting genotypes associated with GC-rich repeats, including Fragile X Syndrome. | 2012-05-03 |
| 20120107825 | METHODS AND COMPOSITIONS FOR ASSESSING PATIENTS WITH REPRODUCTIVE FAILURE USING IMMUNE CELL-DERIVED MICRORNA - The invention is directed to methods and compositions for collecting immune cells, preferably peripheral blood mononuclear cells (PBMCs), before or after an intervention, extracting microRNA-comprising RNA from said cells, quantifying microRNAs within the extracted RNA, determining one or more microRNAs that display a bimodal response amongst a statistically sufficient number of patient samples. Patients are then preferably segregated into groups according to their response. | 2012-05-03 |
| 20120107826 | ZNF217 A New Prognostic And Predictive Biomarker Of Recurrent Invasive And Metastatic Phenotypes In Breast Cancer - The present invention relates to methods for determining the prognosis of a cancer. The methods involve determining the level of expression of the ZNF217 gene in a cancer cell sample or in a tumor sample wherein over-expression of ZNF217 is correlated with likelihood of metastasis and with likelihood of relapse/recurrence of the cancer. | 2012-05-03 |
| 20120107827 | PSMCs As Modifiers of the RB Pathway and Methods of Use - Human PSMC genes are identified as modulators of the RB pathway, and thus are therapeutic targets for disorders associated with defective RB function. Methods for identifying modulators of RB, comprising screening for agents that modulate the activity of PSMC are provided. | 2012-05-03 |
| 20120107828 | METHOD FOR IDENTIFYING A SUBPOPULATION OF MAMMALIAN CELLS WITH DISTINCTIVE RIBOSOME TRANSLATION PROFILES - The present invention generally relates to subpopulations of mammalian cells with distinctive ribosome translational profiles, i.e. translational activities. The present invention further relates to methods for identifying and isolating such cells, kits comprising the same, or methods which utilize different translational activities of these subpopulations of mammalian cells. | 2012-05-03 |
| 20120107829 | STABILIZING COMPOSITIONS FOR IMMOBILIZED BIOMOLECULES - The present invention relates to the use of a composition comprising (a) at least three different amino acids, (b) at least two different amino acids and a saponin or (c) at least one dipeptide or tripeptide for stabilizing biomolecules immobilized on a solid carrier. The invention furthermore relates to a method for producing stabilized biomolecules, comprising embedding the biomolecules in the composition according to the invention and a method of producing a solid carrier having biomolecules attached thereto. The invention furthermore relates to a solid carrier producible or produced by the method of the invention and a method of diagnosing a disease using the carrier of the invention. | 2012-05-03 |
| 20120107830 | REAGENTS FOR BIOMOLECULAR LABELING, DETECTION AND QUANTIFICATION EMPLOYING RAMAN SPECTROSCOPY - The present disclosure provides isotopically substituted compounds of the formula (II): | 2012-05-03 |
| 20120107831 | METHOD FOR DETECTING AFFERENT LYMPH VESSEL INFLOW REGIONS AND METHOD FOR IDENTIFYING SPECIFIC CELLS - Regions where metastatic cancer cells can exist are detected with high accuracy in a sentinel lymph node. Quantum dots are injected into the vicinity of a cancer in a living body, thereby identifying the location of the sentinel lymph node by means of fluorescence. Subsequently, the sentinel lymph node is extracted. With respect to the sentinel lymph node extracted with quantum dots injected, structural analysis is conducted by means of precision fluorescence measurement which uses a confocal fluorescence microscope for monomolecular observation. Specifically, the fluorescence intensity is measured with respect to each of multiple areas in the sentinel lymph nodes, and out of the multiple areas measured, one or more areas are detected as afferent lymph vessel inflow regions in descending order of fluorescence intensity. | 2012-05-03 |
| 20120107832 | METHOD FOR THE DETECTION OF ORGAN OR TISSUE INJURY - This invention relates to methodology for detecting, assessing and/or diagnosing the presence of organ or tissue injury. More specifically, the present invention relates to methodology for detecting particular serum biomarkers that can be used in the detection, assessment and/or diagnosis of organ (e.g. liver) or tissue (e.g. skin) injury. | 2012-05-03 |
| 20120107833 | Antibody specific for a mammalian sphingosine kinase type 2 isoform protein and methods of use thereof - Polyclonal or monoclonal antibodies which are specific for a mammalian sphingosine kinase type 2 isoform protein and methods for detecting the presence of sphingosine kinase type 2 isoform using the antibodies. | 2012-05-03 |
| 20120107834 | ENCAPSULATED REAGENTS AND METHODS OF USE - The present invention contemplates use of encapsulated aqueous and non-aqueous reagents, solutions and solvents and their use in laboratory procedures. These encapsulated aqueous or non-aqueous reagents, solutions and solvents can be completely contained or encapsulated in microcapsules or nanocapsules that can be added to an aqueous or non-aqueous carrier solution or liquid required for medical and research laboratory testing of biological or non-biological specimens. | 2012-05-03 |
| 20120107835 | METHOD FOR DETECTING BETA-AMYLOID USING AMINO ACID DIMERS - A method for detecting β-amyloid using an amino acid dimer instead of a secondary antibody binding to the β-amyloid primary antibody in an immunoassay based on an antigen-antibody reaction, and a kit for detecting β-amyloid including the dimer. The method is advantageous in that the dimer has good properties in stability, availability, and storage compared to antibodies and that it can detect various β-amyloids by using one kind of antibody, and thus may be effectively used in early diagnosis and research of Alzheimer's disease. | 2012-05-03 |
| 20120107836 | DEVELOPMENT OF FLUORESCENTLY P-LOOP LABELLED KINASES FOR SCREENING OF INHIBITORS - The present invention relates to a kinase labelled at an amino acid naturally present or introduced in the P-loop of said kinase, wherein said labelling is effected at a free thiol or amino group of said amino acid and said label is (a) a thiol- or amino-reactive fluorophore sensitive to polarity changes in its environment; or (b) a thiol-reactive spin label, an isotope or an isotope-enriched thiol- or amino-reactive label, such that said fluorophore, spin label, isotope or isotope-enriched label does not inhibit the catalytic activity and does not interfere with the stability of the kinase. The invention furthermore relates to a method of screening for kinase inhibitors, a method of determining the kinetics of ligand binding and/or of dissociation of a kinase inhibitor and a method of generating mutated kinases suitable for the screening of kinase inhibitors using the kinase of the present invention. | 2012-05-03 |
| 20120107837 | METHOD FOR SCREENING ANTICANCER SUBSTANCES, SET OR KIT FOR IMPLEMENTING SAID METHOD - A method for screening anticancer substances including the following steps:
| 2012-05-03 |
| 20120107838 | Cancer Diagnostics - The invention concerns kits and methods for the diagnosis, prognosis and monitoring of cancer. In one aspect, there is provided a method for identifying whether or not a mammal is suffering from cancer, wherein the method comprises the steps of: (a) measuring a signal due to a non-IgG immunoglobulin binding to a carbohydrate-containing antigen in a sample from the mammal; and (b) comparing the signal measured in step (a) with a signal due to the non-IgG immunoglobulin binding to the carbohydrate-containing antigen in one or more samples from one or more mammals known to have cancer and/or with a signal due to the non-IgG immunoglobulin binding to the carbohydrate-containing antigen in one or more samples from one or more healthy mammals. | 2012-05-03 |
| 20120107839 | NMR DEVICE FOR DETECTION OF ANALYTES - This invention relates generally to detection devices having one or more small wells each surrounded by, or in close proximity to, an NMR micro coil, each well containing a liquid sample with magnetic nanoparticles that self-assemble or disperse in the presence of a target analyte, thereby altering the measured NMR properties of the liquid sample. The device may be used, for example, as a portable unit for point of care diagnosis and/or field use, or the device may be implanted for continuous or intermittent monitoring of one or more biological species of interest in a patient. | 2012-05-03 |
| 20120107840 | Methods for Label Free Testing of Cells - The invention provides methods of detecting activation of an immune cell, methods for detecting blocking or enhancing properties of a test reagent or stimuli on activation of one or more immune cells or platelets, methods for selecting hybridomas producing antibodies to an antigen for quality of strength of binding to the antigen, methods for determining if a subject has had an immune response to an immunogen, methods of isolating neutralizing antibodies for an immunogen, methods of classifying a B cell lymphoma, and methods for selecting activated B-cells expressing an antibody to one or more antigens. | 2012-05-03 |
| 20120107841 | Serum Diagnostic Method, Biomarker and Kit for Early Detection and Staging of Alzheimer's Disease - A laboratory method for screening, diagnosing, monitoring and/or staging early onset Alzheimer's disease which consists of mild cognitive impairment entails conducting a blood test after an oxidative exposure of serum to assay for the presence of an elevated level of redox-reactive autoantibodies. | 2012-05-03 |
| 20120107842 | METHOD FOR DETECTING ANTIBODY AGAINST SITH-1 IN BIOLOGICAL SAMPLE - The present invention provides a method for detecting an antibody against a small protein encoded by the intermediate stage transcript of HHV-6 (SITH-1) in a biological sample. | 2012-05-03 |
| 20120107843 | ANTI BONE-LOSS AND ANTI ATTACHMENT-LOSS EFFECTS OF AN ORAL COMPOSITION - Methods for identifying compounds useful for treating diseases and conditions of the oral cavity are described herein. | 2012-05-03 |
| 20120107844 | IMMUNOSORBENT ASSAY SUPPORT AND METHOD OF USE - Embodiments of the present invention provide an immunosorbent assay support immobilized with an intermediate binding antibody and their method of use in an improved immunoassay format. | 2012-05-03 |
| 20120107845 | Disrupting FCRN-Albumin Interactions - Provided herein are, inter alia, methods for identifying a candidate compound for treating the toxic effects of compounds or molecules that bind to albumin in a subject. The methods include identifying test compounds that inhibit the binding between FcRn and albumin. | 2012-05-03 |
| 20120107846 | MEASURING CIRCULATING THERAPEUTIC ANTIBODY, ANTIGEN AND ANTIGEN/ANTIBODY COMPLEXES USING ELISA ASSAYS - The present invention relates to the field of immunology and hyperproliferative diseases. More specifically, the present invention relates to a method of detecting and monitoring therapeutic antibody:antigen complex, soluble antigen and soluble therapeutic antibody, wherein a patient has undergone at least one course of immunotherapy. Yet further, levels of therapeutic antibody:antigen complexes, soluble antigens or soluble therapeutic antibodies may be measured and used to stage or monitor a hyperproliferative disease. | 2012-05-03 |
| 20120107847 | TESTING EFFICACY FOR CELIAC DISEASE - We describe a method to determine effectiveness of a compound or composition in treatment of celiac disease or gluten intolerance, the method comprising:
| 2012-05-03 |
| 20120107848 | POLYCLONAL-MONOCLONAL ELISA ASSAY FOR DETECTING N-TERMINUS PRO-BNP - A specific and sensitive in vitro ELISA assay and diagnostic test kit is disclosed for determining levels of NT-proBNP protein in a variety of bodily fluids, non-limiting examples of which are blood, serum, plasma, urine and the like. The NT-proBNP ELISA assay test employs the sandwich ELISA technique to measure circulating NT-proBNP in human plasma. In order to obtain antibodies with specific binding properties for targeted amino acid sequences within human proBNP, recombinant human proBNP (or rhproBNP) was expressed and purified for use as an immunogen. Polyclonal antibodies (PAb) to specific amino acid sequences were subsequently purified from goat serum by sequential affinity purification. Monoclonal antibodies were raised against specific polypeptides. Recombinant human NT-proBNP (or rhNT-proBNP) was expressed and purified in order to obtain material for use in calibration of a quantitative method for measurement of human NT-proBNP. | 2012-05-03 |
| 20120107849 | COELENTERAZINE DERIVATIVES AND METHODS OF USING SAME - The invention provides coelenterazine derivatives which are substrates for a non-luminescent enzyme and a pro-substrate for a luminescent enzyme. The invention also provides a method of using the derivatives. | 2012-05-03 |
| 20120107850 | ANALYSIS REAGENT AND ANALYSIS DEVICE HAVING THE ANALYSIS REAGENT CARRIED THEREIN - A reagent including a combination of a polyanionic compound and a bivalent cationic compound contains one substance selected from the group consisting of succinic acid, gluconic acid, alanine, glycine, valine, histidine, maltitol, and mannitol or at least one compound of the substance. A dry state of the reagent and deliquescence can be improved. | 2012-05-03 |
| 20120107851 | LATERAL FLOW ASSAY DEVICE FOR COAGULATION MONITORING AND METHOD THEREOF - The present invention is directed to a lateral flow assay device for the monitoring and measuring of coagulation and method thereof. Ideally, the invention is directed to a lateral capillary flow device for the monitoring and/or measurement of coagulation in a liquid sample wherein the device comprises a non-porous substrate with a zone for receiving a sample and a defined flow path zone wherein a clotting agent is deposited on at least part of the defined flow path zone to accelerate the coagulation of the liquid sample, enable the formation of an evenly distributed clot along the defined flow path zone and to result in the change in flow rate or cessation of flow of the liquid sample along the defined flow path zone. | 2012-05-03 |
| 20120107852 | METHOD FOR PREPARING MODIFIED POLYPEPTIDES - Methods for producing polypeptide with altered immunogenicity or improved stability properties are disclosed. The methods involve
| 2012-05-03 |
| 20120107853 | Use of Genetically Modified Organisms to Generate Biomass Degrading Enzymes - The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol. | 2012-05-03 |
| 20120107854 | REAGENT FOR MEASUREMENT OF CHOLINESTERASE ACTIVITY - An N-alkylpiperidine methanethiol ester derivative represented by any one of the general formulae (1) to (4) or a salt thereof has been discovered. The N-alkylpiperidine methanethiol ester derivative or the salt thereof has specificity for acetylcholinesterase or butyrylcholinesterase and is useful as a reagent for measurement of cholinesterase activity by the Ellman method. | 2012-05-03 |
| 20120107855 | CROSSLINKING REAGENT FOR ANALYSIS OF PROTEIN-PROTEIN INTERACTION - Crosslinking reagents and methods for using the same for analysis of protein-protein interactions, are provided. The crosslinking reagents include a trifunctional scaffold that links two protein linking groups to each other and branches to link an affinity tag, where the protein linking groups can be fragmented from the scaffold. The distance between the two protein linking groups can be selected to crosslink two proteins of a protein complex via accessible amino acid residues. Also provided are crosslinked polypeptide compounds and kits that include crosslinking reagents. These reagents and methods find use in a variety of applications in which crosslinking of proteins in desired. | 2012-05-03 |
| 20120107856 | New Fungal Production System - The present invention provides a new fungal production system comprising a fungal host strain of | 2012-05-03 |
| 20120107857 | Di- and Poly-Ubiquitin Deubiquitinase Substrates and Uses Thereof - Methods for detection of the activity of proteolytic enzymes, particularly isopeptidases, are disclosed. | 2012-05-03 |
| 20120107858 | CANCER DIAGNOSIS METHOD USING THE GLYCOSYLATION OF A GLYCOPROTEIN - The present invention relates to a cancer diagnosis method using peptides containing information on the glycosylation of a glycoprotein involving cancer development. More particularly, the present invention relates to a cancer diagnosis method which obtains peptides from the glycoprotein involving cancer development through a hydrolysis process using an enzyme, and quantitatively detects, from among the thus-obtained peptides, glycosylation-related specific peptides which are influenced by the glycosylation of proteins and show specific quantitative changes in the hydrolysis process, to thereby select glycosylation-related specific peptides which show specific quantitative changes in accordance with cancer development. The cancer diagnosis method of the present invention uses the thus-selected glycosylation-related specific peptides as a marker. | 2012-05-03 |
| 20120107859 | METHODS FOR EARLY DETECTION OF BLOOD DISORDERS - Disclosed herein are methods of detecting blood disorders, such as diabetes. In particular examples the method includes contacting a blood sample with an amine reagent, blocking an excess of the amine reagent with a blocking reagent, digesting the modified blood sample with trypsin to produce a digested blood sample containing a plurality of glycated N-terminal peptides and non-glycated N-terminal peptides, then analyzing the digested blood sample with MALDI MS. Also provided are reagents for use in such methods. | 2012-05-03 |
| 20120107860 | CELL SORTING APPARATUS, CELL SORTING CHIP, AND CELL SORTING METHOD - Disclosed herein is a cell sorting apparatus, including: a branch portion branching a flow path in which a fluid containing therein cells flows into a first branch flow path, and a second branch flow path; a coupling portion coupling the first branch flow path and the second branch flow path to each other; and a flowing-out portion causing liquids flowing in the first branch flow path and the second branch flow path coupled to each other by the coupling portion, respectively, to flow out to an outside. | 2012-05-03 |
| 20120107861 | METHOD OF DETERMINING RISK OF ARRHYTHMIA - The present invention relates to a method of determining the risk of drug induced arrhythmia using stem cell derived cardiomyocytes in a high-throughput impedance or multi-electrode array assay. | 2012-05-03 |
| 20120107862 | FACTORS - A method for determining a prognosis for benefit for a cancer patient receiving immunotherapy treatment involving (a) measuring a level of haematocrit and haemoglobin in a sample from the cancer patient, and (b) comparing the level of haematocrit in the sample to a reference level of platelets and comparing the level of haemoglobin in the sample to a reference level of haemoglobin, wherein a lower level of haematocrit and higher level of haemoglobin in the sample correlates with increased benefit to the patient. | 2012-05-03 |
| 20120107863 | MODULATION OF TELOMERE LENGTH IN TELOMERASE POSITIVE CELLS AND CANCER THERAPY - Induction of telomere shortening, G2 arrest and apoptosis in telomerase positive cancer cells using acyclic nucleoside analogs has been disclosed. In addition, methods for impairment or prevention of tumorigenic telomerase positive cells from having a chance to grow into a tumor and methods for promoting tumor regression (decrease in size of an established tumor) using acyclic nucleoside analogs has been disclosed. | 2012-05-03 |
| 20120107864 | MASS SPECTROMETRIC RAPID DETECTION OF SALMONELLA - The invention relates to the detection of specified, flagellated bacteria, particularly | 2012-05-03 |
| 20120107865 | DETECTION OF BACTERIA AND FUNGI - A method of detecting a ligase expressing micro-organism in a sample comprises steps of treating the sample under conditions that inhibit the activity of ATP-dependent ligase from mammalian cells but which do not inhibit the activity of the microbial ligases, contacting the sample or a portion of the sample with a nucleic acid molecule which acts as a substrate for ligase activity in the sample, incubating the thus contacted sample under conditions suitable for ligase activity; and specifically determining the presence and/or the amount of a ligated nucleic acid molecule resulting from the action of the ligase on the substrate nucleic acid molecule to indicate the presence of the ligase expressing micro-organism. The micro-organism may be a fungus or a bacterium or both. High pH conditions may be employed to inactivate mammalian ligases. Related kits are described. | 2012-05-03 |
| 20120107866 | METHODS TO INCREASE AND HARVEST DESIRED METABOLITE PRODUCTION IN ALGAE - Enhanced yields of photosynthetically fixed carbon produced by hypersaline photosynthetic algae are provided by co-culturing with a halophilic archaea. Further, methods are provided to control harvesting of desired metabolic products from hypersaline photosynthetic algae by controlling caspase activity. | 2012-05-03 |
| 20120107867 | GLYCOPEGYLATED ERYTHROPOIETIN - The present invention provides conjugates between erythropoietin and PEG moieties. The conjugates are linked via an intact glycosyl linking group interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from glycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto a glycosyl residue on the peptide. Also provided are methods for preparing the conjugates, methods for treating various disease conditions with the conjugates, and pharmaceutical formulations including the conjugates. | 2012-05-03 |
| 20120107868 | PROCESSES FOR MAKING PROTEIN HYDROLYSATES FROM ANIMAL PEPTONE AND FOR PRESERVING MUCOSA - A process for hydrolyzing products with enzymatic activity remaining in peptone solutions after mucosa hydrolysis is provided along with a process for preserving mucosa tissue. Broadly, the processes are carried out by hydrolyzing mucosa tissue according to conventional heparin manufacturing processes wherein an excess quantity of proteolytic enzymes is used. The resulting peptone solution is then contacted with proteins or protein-containing materials in order to hydrolyze the proteins. In another embodiment, mucosa tissue is preserved by mixing it with a preserving agent selected from the group consisting of hydrogen peroxide and phosphoric acid. The product preserved by hydrogen peroxide is low in ash, stable for at least a week, and has a reduced odor. | 2012-05-03 |
| 20120107869 | ORGANIC COMPOUNDS - Disclosed is a method for the production of a heterologous polypeptide of interest with a homogenous N-terminus, using a fusion polypeptide comprising the polypeptide of interest and N-terminally thereto a polypeptide exhibiting autoproteolytic function, said method comprising the steps of a) binding of the fusion polypeptide in a soluble, autoproteolytically inactive form by an affinity chromatography system, b) refolding of the fusion polypeptide, thereby activating the autoproteolytic function of the fusion polypeptide and causing cleavage of the heterologous polypeptide of interest, and c) subsequently eluting the heterologous polypeptide of interest, wherein said steps are conducted on one affinity chromatography system. | 2012-05-03 |
| 20120107870 | SELECTIVE ENZYMATIC AMIDATION OF C-TERMINAL ESTERS OR ACIDS OF PEPTIDES - The present invention relates to a process for the amidation of C-terminal esters or acids of peptide substrates in solution-phase synthesis of peptides, comprising amidating one or more peptide substrates comprising C-terminal esters or acids using the protease subtilisin in any suitable form in the presence of an ammonium salt derived from an acid having a pKa above 0. | 2012-05-03 |
| 20120107871 | METHOD OF PRODUCING HUMAN IGG ANTIBODIES WITH ENHANCED EFFECTOR FUNCTIONS - A method for generating human IgG | 2012-05-03 |
| 20120107872 | THERMOSTABLE TRICHODERMA CELLULASE - Described are compositions and methods relating to the thermostable fungal cellulase enzyme, EGV, and | 2012-05-03 |
| 20120107873 | Method of Producing a Sweet Protein - The present invention relates to a method of producing a recombinant sweet protein in a filamentous fungus. The invention also relates to isolated polynucleotides encoding the sweet protein and to nucleic acid constructs, vectors, and host cells comprising the polynucleotides, as well as methods of using the sweet protein produced by the method of the invention. | 2012-05-03 |
| 20120107874 | Modified Host Cells and Uses Thereof - The present disclosure provides compositions and methods comprising cells producing glycoproteins with variant glycosylation patterns. The methods and compositions may be used in producing antibodies and proteins of therapeutic value. | 2012-05-03 |
| 20120107875 | Expression Cassette, Recombinant Host Cell and Process for Producing a Target Protein - Disclosed herein is a process for producing a target protein, in which a recombinant polynucleotide is constructed to encode a fusion protein including: (i) an anchoring protein that includes a N-terminal amino acid sequence of an ice nucleation protein, so that the fusion protein, once expressed in the host cell, is directed by the anchoring protein to be anchored and exposed on the outer membrane of the host cell; (ii) the target protein; and (iii) a self-splicing protein that includes a first end fused with the anchoring protein and a second end fused with the target protein, wherein the self-splicing protein includes a N-terminal or C-terminal amino acid sequence of an intein protein at the second end thereof, such that upon an environmental stimulus, the self-splicing protein exerts a self-cleavage at the second end thereof to release the target protein from the fusion protein. | 2012-05-03 |
| 20120107876 | NOVEL FUSION TAG OFFERING SOLUBILITY TO INSOLUBLE RECOMBINANT PROTEIN - The invention relates to a fusion tag comprising Serine-aspartic acid repeats of the well conserved region of the | 2012-05-03 |
| 20120107877 | Methods and Compositions for Concentrating Secreted Recombinant Protein - Methods and compositions are described that relate to obtaining concentrated preparations of secreted recombinant proteins. These proteins are expressed in the form of fusion proteins with a chitin-binding domain (CBD). The fusion proteins are capable of being concentrated in the presence of chitin. Also described is: a shuttle vector that includes a modified LAC4 promoter; a chitinase-negative host cell; a CBD capable of eluting from chitin under non-denaturing conditions; and sterilized chitin, which can be optionally magnetized for facilitating recovery of recombinant protein. | 2012-05-03 |
| 20120107878 | MULTIPLEX ASSEMBLY OF HIGH FEDELITY DNA - The present invention relates to novel sequence normalization protocols and methods which utilize this protocol to provide a robust multiplexed assembly of high fidelity polynucleotides/genes | 2012-05-03 |
| 20120107879 | METHODS FOR PRODUCTION AND PURIFICATION OF NUCLEIC ACID MOLECULES - The present invention is directed to methods for the production and isolation of nucleic acid molecules. In particular, the invention concerns isolation of mRNA molecules and the production and isolation of nucleic acid molecules (e.g., cDNA molecules or libraries), which may be single- or double-stranded. Additionally, the invention concerns selection and isolation of particular nucleic acid molecules of interest from a sample which may contain a population of molecules. Specifically, the invention concerns affinity-labeled primer-adapter molecules which allow improved isolation and production of such nucleic acid molecules, increasing both product recovery and speed of isolation. | 2012-05-03 |
| 20120107880 | FUNGAL STRAINS - The present invention provides improved fungal strains. In some embodiments, the improved fungal strains find use in hydrolyzing cellulosic material to glucose. | 2012-05-03 |
| 20120107881 | COMPOSITIONS AND METHODS FOR PRODUCTION OF FERMENTABLE SUGARS - The present application provides genetically modified fungal organisms that produce enzyme mixtures exhibiting enhanced hydrolysis of cellulosic material to glucose, enzyme mixtures produced by the genetically modified fungal organisms, and processes for producing glucose from cellulose using such enzyme mixtures. | 2012-05-03 |
| 20120107882 | Recombinant Microorganism And Method For Producing L-Lysine - The present invention provides methods of producing L-lysine at a high yield using an | 2012-05-03 |
| 20120107883 | METHOD FOR PRODUCING 4-HYDROXY-L-ISOLEUCINE - A highly active L-isoleucine dioxygenase from | 2012-05-03 |
| 20120107884 | METHOD FOR PRODUCING 4-HYDROXY-L-ISOLEUCINE - A highly active L-isoleucine dioxygenase from | 2012-05-03 |
| 20120107885 | Anaerobic Fermentation of Glycerol - The invention relates to the development of appropriate cultivation conditions for a bacteria to grow anaerobically (fermentatively) on a glycerol substrate. The method requires culturing bacteria having a functional 1,2-propanediol pathway and a functional type II glycerol dehydrogenase-dihydroxyacetone kinase pathway in a culture medium containing high concentrations of glycerol, a neutral to mildly acidic pH, low levels of potassium and phosphate, and high levels of CO2, such that glycerol is thus converted into a desirable product, such as ethanol, hydrogen, formate, succinate, or 1,2-propanediol. | 2012-05-03 |
| 20120107886 | Lignin Oxidation and Products Thereof - Provided herein is a method of oxidizing lignin. Further disclosed herein are aromatic and non-aromatic compounds obtained from oxidized lignin. | 2012-05-03 |
| 20120107887 | PROCESS FOR THE PRODUCTION OF ALCOHOLS FROM BIOMASS - Alcohols useful as fuel compositions are produced from biomass by: contacting the biomass with a cooking liquor containing 1 to 20 wt %, based on the cooking liquor, of sodium hydroxide, and under conditions effective to provide a pulp stream containing pulp having a lignin content of 5% to 20% by weight, based on the pulp washing the pulp stream; hydrolyzing the washed pulp stream with an enzyme solution; fermenting the hydrolyzate in the presence of a microorganism, one alcohol having 2 to 18 carbon atoms. | 2012-05-03 |
| 20120107888 | MODULATION OF FERMENTATION PRODUCTS THROUGH VITAMIN SUPPLEMENTATION - Improved yields of biofuels and other chemicals are obtained by culturing a cellulolytic microorganism, such as | 2012-05-03 |
| 20120107889 | ENDOPHYTIC YEAST STRAINS, METHODS FOR ETHANOL AND XYLITOL PRODUCTION, METHODS FOR BIOLOGICAL NITROGEN FIXATION, AND A GENETIC SOURCE FOR IMPROVEMENT OF INDUSTRIAL STRAINS - The present invention provides novel endophytic yeast strains capable of metabolizing both pentose and hexose sugars. Methods of producing ethanol and xylitol using the novel endophytic yeast are provided herein. Also provided are methods of fixing nitrogen and fertilizing a crop using the novel endophytic yeast strains provided herein. | 2012-05-03 |
| 20120107890 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 2012-05-03 |
| 20120107891 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 2012-05-03 |
| 20120107892 | PRODUCTION OF ETHANOL FROM LIGNOCELLULOSIC BIOMASS - Described herein are methods for converting lignocellulosic biomass to ethanol, comprising the step of contacting the lignocellulosic biomass with a mixture for a period of time at an initial temperature and an initial pH, wherein the mixture comprises a first microorganism and a second microorganism, thereby producing an amount of ethanol. The first microorganism or the second microorganism may be a thermophilic or mesophilic microorganism. The first microorganism may be a native cellulolytic microorganism or a native xylanolytic microorganism; and the second microorganism may be a genetically engineered xylanolytic microorganism or a genetically engineered cellulolytic microorganism. The microorganisms may be | 2012-05-03 |
| 20120107893 | MICROBIAL ENGINEERING FOR THE PRODUCTION OF CHEMICAL AND PHARMACEUTICAL PRODUCTS FROM THE ISOPRENOID PATHWAY - The invention relates to the production of one or more terpenoids through microbial engineering, and relates to the manufacture of products comprising terpenoids. | 2012-05-03 |
| 20120107894 | Methods and Compositions for the Recombinant Biosynthesis of Terminal Olefins - The present disclosure identifies methods and compositions for modifying microbial cells, such that the organisms efficiently synthesize terminal olefins, and in particular the use of such organisms for the commercial production of propylene and related molecules. | 2012-05-03 |
| 20120107895 | Method and Apparatus for Membrane-Based, Two-Stage Gas Production from Solid Biomaterials - Embodiments of the present invention preferably relate to a method and apparatus for a two-stage membrane-based production of gas, preferably hydrogen gas or the like, from solid biological materials, preferably organic waste materials or the like, comprising anaerobic hydrolysis and fermentation and photofermentation using microorganisms. | 2012-05-03 |
| 20120107896 | Method for Treating a Biological Material Comprising Living Cells - A plasma containing at least one reactive species are used to deliver agents into living cells or an extracellular matrix of the living cells. The method and system allows for treatment of the living cells without causing irreversible damage to the cell membranes of the living cells. The plasma is produced by dielectric barrier discharge. | 2012-05-03 |
| 20120107897 | DOUBLE-STRANDED OLIGONUCLEOTIDES - Antisense sequences, including duplex RNAi compositions, which possess improved properties over those taught in the prior art are disclosed. The invention provides optimized antisense oligomer compositions and method for making and using the both in in vitro systems and therapeutically. The invention also provides methods of making and using the improved antisense oligomer compositions. | 2012-05-03 |
| 20120107898 | METHOD FOR OBTAINING HUMAN MICROGLIAL PRECURSOR CELLS FROM PLURIPOTENT STEM CELLS - A method for obtaining human microglial precursor cells, comprising: (a) providing a cell population comprising neural precursor cells, wherein the cell population is obtainable from embryoid bodies differentiated from human pluripotent stem cells; (b) differentiating the cell population comprising neural precursor cells into microglial precursor cells by culturing in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors; (c) expanding and enriching microglial precursor cells in medium comprising a growth factor selected from the group consisting of insulin and insulin-like growth factors and 10 to 150 ng/ml GM-CSF; and (d) isolating microglial precursor cells comprising CD45-positive cells. | 2012-05-03 |
| 20120107899 | Heat-Stable Carbonic Anhydrases and Their Use - The present invention relates to use of | 2012-05-03 |
| 20120107900 | Electrospun Polymer Fibers Comprising Particles of Bacteria-Containing Hydrogels - The present invention provides electrospun polymer fibers comprising bacteria-containing hydrogel particles. Bacteria-containing hydrogel particles are produced by crosslinking water-soluble polymers to form hydrogels and mixing them with a bacteria suspension. The crosslinking is suitable to be carried out either chemically before the addition of the bacteria suspension or physically before or after this addition. Subsequently, these hydrogel particles are electrospun together with an electrospinnable polymer solution to form fibers or fibre non-wovens. | 2012-05-03 |
| 20120107901 | METHOD OF TREATING THE SURFACE OF A MEDICAL DEVICE WITH A BIOMOLECULE - A method of treating the surface of a medical device with a biomolecule comprising the steps of: providing a polyolefin substrate forming a medical device; cleaning the polyolefin substrate; exposing the polyolefin substrate to a reactive gas containing acrylic acid and to plasma energy to yield a plasma-deposited polyacrylic acid coating on the polyolefin substrate; and attaching a biomolecule, such as heparin, to the polyolefin substrate following formation of the plasma-deposited polyacrylic acid coating on the polyolefin substrate. | 2012-05-03 |
| 20120107902 | Processes For Forming Amide Bonds And Compositions Related Thereto - The disclosure relates to methods for producing amide bonds and reagents related thereto. In some embodiments, the disclosure relates to methods of producing an amide comprising mixing an O-silylated thionoester and an amine under conditions such that an amide is formed. In another embodiment, the disclosure relates to mixing a thiolacid, a silylating agent, and an amine under conditions such that an amide is formed. | 2012-05-03 |
| 20120107903 | Mutant Glucose Dehydrogenase - A mutant glucose dehydrogenase having an amino acid sequence at least 80% identical to SEQ ID NO:3 and having glucose dehydrogenase activity, wherein amino acid residues corresponding to positions 326, 365 and 472 of said amino acid sequence are replaced with glutamine, tyrosine and tyrosine, respectively, and wherein said mutant glucose dehydrogenase shows an improved substrate specificity to glucose and a reduced reactivity to disaccharides. | 2012-05-03 |
| 20120107904 | CARBOXY TERMINAL RESIDUES AS PREDICTORS AND REGULATORS OF PROTEIN STABILITY - Compositions and methods are provided that are useful for predicting and controlling the stability of expressed polypeptides. The compositions and methods may be used to predict and as desired, increase or decrease the stability of proteins recombinantly expressed in mycobacteria, for example DesA3 expressed in | 2012-05-03 |
| 20120107905 | Variants of Fungal Serine Protease - The present invention relates to fungal serine protease variants, which comprise an amino acid substitution of valine at position 208 of the parent | 2012-05-03 |
| 20120107906 | NOVEL LIPOLYTIC ENZYME LIP1 - The present invention provides a novel nucleic acid sequence, designated LIP1, encoding a lipolytic enzyme and the corresponding encoded amino acid sequences. The invention also provides expression vectors and host cells comprising a nucleic acid sequence encoding at least one novel lipolytic enzyme, recombinant lipolytic enzyme proteins and methods for producing the same. | 2012-05-03 |
| 20120107907 | THERMOSTABLE NEUTRAL METALLOPROTEASES - The present invention provides methods and compositions comprising at least one neutral metalloprotease enzyme that has improved storage stability. In some embodiments, the neutral metalloprotease finds use in cleaning and other applications. In some particularly preferred embodiments, the present invention provides methods and compositions comprising neutral metalloprotease(s) obtained from | 2012-05-03 |
| 20120107908 | Enzymatic Formulation and Composition for CO2 Capture Reactions - A triphasic bioreactor and process for physico-chemically treating a gas includes a reaction chamber and its use with a liquid and biocatalysts in suspension, for catalyzing a reaction between the gas and the liquid to obtain a treated gas and a solution containing a reaction product. An enzymatic formulation for catalysis of the reaction CO | 2012-05-03 |
| 20120107909 | NOVEL HIV-2 ISOLATE - The invention provides a novel strain of HIV-2 capable of causing immunodeficiency. The invention also provides compositions comprising the nucleic acids and polypeptides characteristic of this HIV-2 virus, antibodies specific for this HIV-2 virus, methods of using these compositions, and methods of detecting HIV-2 virus. | 2012-05-03 |
| 20120107910 | RECOMBINANT ENVELOPE PROTEIN OF HUMAN IMMUNODEFICIENCY VIRUS (HIV) AND VACCINE CONTAINING THE SAME - The present invention provides a recombinant HIV Env antigenic protein, a virus-like particle and a recombinant HIV virus. The present invention further provides a vaccine comprising the recombinant HIV Env antigenic protein, the virus-like particle or recombinant HIV virus. | 2012-05-03 |
| 20120107911 | RECOMBINANT HEMAGGLUTININ PROTEIN OF INFLUENZA VIRUS AND VACCINE CONTAINING THE SAME - The present invention provides a recombinant hemagglutinin antigenic protein, a virus-like particle and a recombinant influenza virus. The present invention further provides a vaccine comprising the recombinant hemagglutinin antigenic protein, the virus-like particle or recombinant influenza virus. | 2012-05-03 |
| 20120107912 | CELL LYSIS DEVICE AND METHODS OF LYSING CELLS OR VIRUSES - A method of lysing at least one of a cell and a virus, the method including: contacting a sample, which includes at least one of a cell and a virus, with a plurality of beads which are disposed in a first chamber to obtain a combination of the sample and the beads; and agitating the combination of the sample and the beads to lyse the at least one of the cell and the virus, wherein in the first chamber a liquid volume fraction is 0.6 or less, and wherein the liquid volume fraction is a value obtained by dividing a liquid volume of the first chamber by a pure void volume equivalent to a sum of the liquid volume of the first chamber and a void volume of the first chamber. | 2012-05-03 |
| 20120107913 | MICROORGANISM CULTURE SHEET AND MANUFACTURING METHOD THEREFOR - A microorganism culture sheet is provided which can limit the use of a costly cultivation material to a necessary portion, which has simple production at the time of manufacture, and can be simply and stably used by a user. The microorganism culture sheet of the present invention is a microorganism culture sheet comprising a base sheet, a culture layer formed on top of the base sheet, and a cover sheet that covers the culture layer, and the culture layer is pattern-formed with an application liquid comprising a polyvinylpyrrolidone and at least on selected from the group consisting of a gelling agent, a nutrient component, a color indicator, a selective agent and a substrate. | 2012-05-03 |
| 20120107914 | MICROBIAL ACTIVITY IMPROVEMENT AGENT, MICROBIAL ACTIVITY IMPROVEMENT METHOD, AND BIOLOGICAL WASTE TREATMENT METHOD - A compound selected from the group consisting of 4-quinolone and derivatives thereof, homoserine lactone derivatives, and indole and derivatives thereof, and salts thereof have an action of a microbial activity improvement agent, and when it is used for a microbial activity improvement method and a biological waste treatment method, the compound or the salt thereof is useful to achieve the improvement in the treatment capacity that transcends the limit of improvement in the treatment capacity of conventional biological waste treatment facilities. | 2012-05-03 |
| 20120107915 | METHOD FOR HIGH-THROUGHPUT IDENTIFICATION OF MICROBIAL ANTAGONISTS AGAINST PATHOGENS - The present invention relates to high-throughput methods of screening biological samples to identify microorganisms having potential utilities as biocontrol agents. The methods include, for example, the use of multitest platforms for the simultaneous identification of microorganisms having biocontrol activity, including those useful in improving plant, animal, and human health. In particular, the present invention provides screening methods suitable for identification of microorganisms having potential applications in combating diseases caused by plant pathogens. The disclosure also provides microorganisms having biocontrol activity that are identified by the screening methods disclosed herein. | 2012-05-03 |
