17th week of 2015 patent applcation highlights part 43 |
Patent application number | Title | Published |
20150111195 | Cell Washing Device Using a Wave - A system is disclosed for washing a selected component and removing the selected component from a wash material. The selected component may include red blood cells that are washed to remove a rejuvenating solution. The red blood cells may then be removed with various systems, such as a standing acoustic wave system from the wash solution. Pumps and flow restrictors that provide steady flow from pumps that generate pulsed flow are also disclosed. | 2015-04-23 |
20150111196 | MICROFLUIDIC CHIP - A microfluidic chip orients and isolates components in a sample fluid mixture by two-step focusing, where sheath fluids compress the sample fluid mixture in a sample input channel in one direction, such that the sample fluid mixture becomes a narrower stream bounded by the sheath fluids, and by having the sheath fluids compress the sample fluid mixture in a second direction further downstream, such that the components are compressed and oriented in a selected direction to pass through an interrogation chamber in single file formation for identification and separation by various methods. The isolation mechanism utilizes external, stacked piezoelectric actuator assemblies disposed on a microfluidic chip holder, or piezoelectric actuator assemblies on-chip, so that the actuator assemblies are triggered by an electronic signal to actuate jet chambers on either side of the sample input channel, to jet selected components in the sample input channel into one of the output channels. | 2015-04-23 |
20150111197 | NOVEL ISOLATED AND PURIFIED STRAINS OF CHIKUNGUNYA VIRUS AND POLYNUCLEOTIDES AND POLYPEPTIDES SEQUENCES, DIAGNOSTIC AND IMMUNOGENICAL USES THEREOF - The present invention concerns wild-strains of Chikungunya virus isolated from patients exhibiting severe forms of infection and stemming from a human arbovirosis epidemy. The present invention also concerns polypeptide sequences and fragment thereof derived from their genome, the polynucleotide encoding same and their use as diagnostic products, as vaccine and/or as immunogenic compositions. | 2015-04-23 |
20150111198 | MEDICAL ANALYSIS METHOD - A medical analysis method uses a medical analysis machine provided with a poly-articulated robot ( | 2015-04-23 |
20150111199 | Methods of Using Near Field Optical Forces - Methods of studying, interrogating, analyzing, and detecting particles, substances, and the like with near field light are described. Methods of identifying binding partners, modulators, inhibitors, and the like of particles, substances, and the like with near field light are described. In certain embodiments, the methods comprise immobilizing or trapping the particle, substance, and the like. | 2015-04-23 |
20150111200 | Luminescence Reference Standards - The present teachings provide for systems, and components thereof, for detecting and/or analyzing light. These systems can include, among others, optical reference standards utilizing luminophores, such as nanocrystals, for calibrating, validating, and/or monitoring light-detection systems, before, during, and/or after sample analysis. | 2015-04-23 |
20150111201 | PORTABLE RAPID DIAGNOSTIC TEST READER AND METHODS OF USING THE SAME - A portable rapid diagnostic test reader system includes a mobile phone having a camera and one or more processors contained within the mobile phone and a modular housing configured to mount to the mobile phone. The modular housing including a receptacle configured to receive a sample tray holding a rapid diagnostic test. At least one illumination source is disposed in the modular housing and located on one side of the rapid diagnostic test. An optical demagnifier is disposed in the modular housing interposed between the rapid diagnostic test and the mobile phone camera. | 2015-04-23 |
20150111202 | AUTOMATED STAINING SYSTEM AND REACTION CHAMBER - An apparatus including a reagent cartridge and a reaction chamber, the reagent cartridge having a reagent capsule removably positioned therein for dispensing of a reagent onto the reaction chamber. A system including a linearly translatable mounting assembly having a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge, a linearly translatable bulk reagent dispensing assembly having a plurality of bulk reagent dispensing nozzles coupled thereto and a receiving assembly positioned beneath the mounting assembly and the bulk reagent dispensing assembly, the receiving assembly including a plurality of reaction stations. A method including determining an inventory of an automated sample processing system, downloading a processing protocol from a central controller to the automated sample processing system, operating the automated sample processing system based on the processing protocol and independently of the central controller and dispensing a reagent from the automated sample processing system. | 2015-04-23 |
20150111203 | METHODS FOR DETERMINING CARRIER STATUS - The invention generally relates to methods for determining carrier status with respect to a condition or disease. In certain embodiments, the method involves exposing a sample to a plurality of molecular inversion probes capable of capturing DNA from at least one genomic region suspected of having an altered copy number and at least one internal control DNA known or suspected to have a stable copy number, capturing and sequencing DNA that binds to the molecular inversion probes, and determining a copy number state of the at least one genomic region based on the sequence results. | 2015-04-23 |
20150111204 | Single Molecule Sequencing of Captured Nucleic Acids - The invention provides methods and devices for detecting, enumerating or identifying target nucleic acid molecules using immobilized capture probes and single molecule sequencing techniques. | 2015-04-23 |
20150111205 | Methods for Mapping Bar-Coded Molecules for Structural Variation Detection and Sequencing - The invention includes methods for optimally designing probes and analyzing data from sequence-by-hybridization and related methods of stretched molecules or other experimental approaches that provide local information. | 2015-04-23 |
20150111206 | DETECTION METHOD FOR MYOCOBACTERIUM AVIUM SPP. PARATUBERCULOSIS - The invention relates to a method for specifically detecting and optionally for quantifying | 2015-04-23 |
20150111207 | Detection of Mycoplasma in Cell Cultures and Cell Culture derived Biologicals - Sequences having specificity for | 2015-04-23 |
20150111208 | METHODS FOR ASSESSING A GENOMIC REGION OF A SUBJECT - The invention generally relates to method for assessing a genomic region of a subject. In certain embodiments, methods of the invention involve obtaining a sample including nucleic acid from a subject. The nucleic acid includes a target sequence from a target genomic region and a paralogous sequence from a non-target genomic region. The target sequence and the paralogous sequence are isolated from the sample. The target sequence and the paralogous sequence are sequenced to obtain sequence reads that include target sequence reads and paralogous sequence reads. The paralogous sequence reads are excluded, and the genomic region of the subject are assessed based on the target sequence reads. | 2015-04-23 |
20150111209 | Gene Amplification of Coactivator COAA and Uses Thereof - It has been discovered that amplifications in the gene coactivator activator (CoAA) blocks stem cell differentiation and induces cancer stem cells. One embodiment provides compositions and methods for treating or alleviating one or more symptoms associated with cancer due to gene amplifications in CoAA. Another embodiment provides methods and compositions for detecting cancer due to gene amplifications in CoAA. Still another embodiment provides methods for identifying compounds, antibodies and nucleic acid molecules that are useful for treating cancer due to gene amplifications in CoAA. Preferably the disclosed compositions antagonize or interfere with the CoAA amplicons and the biological activity of CoAA and or splice variants thereof. | 2015-04-23 |
20150111210 | DETECTION OF SHIGA TOXIN GENES IN BACTERIA - The disclosed invention is related to methods, compositions and kits for targeting nucleic acid derived from Shiga toxin-producing bacteria such as | 2015-04-23 |
20150111211 | PREDICTING RESPONSE TO A HER INHIBITOR - The present application describes the use of low HER3 as a selection criterion for treating patients with a HER inhibitor, such as pertuzumab. | 2015-04-23 |
20150111212 | COMPOSITIONS, KITS AND RELATED METHODS FOR THE DETECTION AND/OR MONITORING OF PSEUDOMONAS AERUGINOSA - The present invention provides compositions, methods and kits for the species-specific detection of | 2015-04-23 |
20150111213 | RAD51C AS A HUMAN CANCER SUSCEPTIBILITY GENE - The invention discloses in vitro methods and a system for determining a predisposition of a subject for developing a cancer on the basis of analyzing a sample of the subject for an alteration of at least one allele of the RAD51C gene. Further disclosed are in vitro methods for assessing clinical features or a pathological progression of a cancer and for assessing at least one RAD51 C gene alteration in a cell. In addition a kit for determining a predisposition of a subject for developing a cancer, and certain uses of oligonucleotides for determining the presence of at least one mono-allelic germ-line mutation of the RAD51C gene. | 2015-04-23 |
20150111214 | DIRECT QUANTIFICATION OF UNPROCESSED NUCLEIC ACID SAMPLES - A workflow for direct qPCR quantification of unprocessed forensic casework samples is disclosed herein. 13 pg of DNA has been detected by direct amplification from a paper substrate. Direct qPCR quantification of unprocessed forensic casework samples and direct STR amplification of unprocessed forensic casework samples collected on the same PE-swab will greatly increase forensic laboratory's efficiency and capability. | 2015-04-23 |
20150111215 | SAMPLE HANDLING - The invention provides containers and methods of use for the storage, transportation and preparation of samples, such as DNA samples for analysis. The container is pre-provided with the reagents in sealed chambers. The sample can be introduced and the container manipulated to release the reagents, provide the necessary conditions and give a fully prepared sample. The container can then be engaged with an analysis device to identify characteristics of the sample or perform other operations thereon. | 2015-04-23 |
20150111216 | DEVICES AND METHODS FOR STAINING AND MICROSCOPY - Devices and methods for preparing and assessing a liquid sample are described which include: a microfluidic microscopy device including an absorbent structure including at least one dry dye for a liquid and configured to convey a polar liquid from a first end of the absorbent structure through the at least one dry dye for a liquid to a second end of the absorbent structure, a support structure positioned adjacent to a first surface of the absorbent structure, a lid structure positioned adjacent to a second surface of the absorbent structure, and a clamping structure positioned to maintain a relative position of the support structure and the lid structure to form a tapered internal chamber adjacent to the second end of the absorbent structure. | 2015-04-23 |
20150111217 | MARKER QUANTITATION IN SINGLE CELLS IN TISSUE SECTIONS - Improved assays incorporating single-cell based image analyses that enable quantitation of expression of individual cellular proteins and heterogeneity in terms of individual cellular protein molecule numbers per cell at the single cell level and mapped across sections of clinical tissue samples are disclosed. | 2015-04-23 |
20150111218 | CELL DIFFERENTIATION ASSAY METHOD, CELL ISOLATION METHOD, METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS, AND METHOD FOR PRODUCING DIFFERENTIATED CELLS - Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell. (A) A protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc;” and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc.” | 2015-04-23 |
20150111219 | LIPOSOMAL COMPOSITION COMPRISING A STEROL-MODIFIED LIPID AND A PURIFIED MYCOBACTERIAL LIPID CELL WALL COMPONENT AND ITS USE IN THE DIAGNOSIS OF TUBERCULOSIS - A liposomal composition comprising a sterol-modified lipid and a purified mycobacterial lipid cell wall component or analog or derivative thereof is described. The composition is useful as a lipid antigen-presenting vehicle for the detection of lipid antigen specific biomarker antibodies in antibody containing biological samples in the diagnosis of active tuberculosis. The purified lipid cell wall component is typically a purified mycolic acid or a mixture of mycolic acids from a | 2015-04-23 |
20150111220 | METHOD FOR EVALUATION OF PRESENCE OF OR RISK OF COLON TUMORS - The disclosed methods are used to predict or assess colon tumor status in a patient. They can be used to determine nature of tumor, recurrence, or patient response to treatments. Some embodiments of the methods include generating a report for clinical management. The methodology provided herein is intended to detect technical variations and to allow for data normalization and enhance signal detection and build predictive proteins profiles of disease status and response. | 2015-04-23 |
20150111221 | METHOD FOR EVALUATION OF PRESENCE OF OR RISK OF COLON TUMORS - The disclosed methods are used to predict or assess colon tumor status in a patient. They can be used to determine nature of tumor, recurrence, or patient response to treatments. Some embodiments of the methods include generating a report for clinical management. The methodology provided herein is intended to detect technical variations and to allow for data normalization and enhance signal detection and build predictive proteins profiles of disease status and response. | 2015-04-23 |
20150111222 | GENETICALLY ENCODED BIOSENSORS - The present disclosure provides, inter alia, genetically encoded recombinant peptide biosensors comprising analyte-binding framework portions and signaling portions, wherein the signaling portions are present within the framework portions at sites or amino acid positions that undergo a conformational change upon interaction of the framework portion with an analyte. | 2015-04-23 |
20150111223 | METHOD FOR EVALUATION OF PRESENCE OF OR RISK OF COLON TUMORS - The disclosed methods are used to predict or assess colon tumor status in a patient. They can be used to determine nature of tumor, recurrence, or patient response to treatments. Some embodiments of the methods include generating a report for clinical management. The methodology provided herein is intended to detect technical variations and to allow for data normalization and enhance signal detection and build predictive proteins profiles of disease status and response. | 2015-04-23 |
20150111224 | BIOMARKERS FOR ADVERSE CARDIAC REMODELING - The present invention provides for certain biomarkers for adverse cardiac remodeling. The biomarkers have predictive value in assessing the risk of a subject developing heart failure and other conditions related to adverse cardiac remodeling, as well as diagnosing adverse cardiac remodeling, and determining response to therapy addressing adverse cardiac remodeling. | 2015-04-23 |
20150111225 | METHOD FOR IN VITRO DIFFERENTIATION OF BLOOD CIRCULATING CELLS INTO NEURONAL-LIKE CELLS AND APPLICATIONS THEREOF - The present invention relates to a method for in vitro differentiation of a population of blood circulating cells, such as monocytes and preferably pluripotent macrophages derived therefrom, into cells displaying functional and phenotypic neuronal characteristics. The invention further encompasses neuronal-like cells obtainable according to the present method, compositions comprising said cells, and applications thereof. | 2015-04-23 |
20150111226 | METHOD FOR PEPTIDE HISTOCHEMICAL DIAGNOSIS - The present invention provides a method of peptide histochemical diagnosis to detect the peptide binding protein in the cancer tissue. This peptide binding specifically to tumor cells is linked to the dextran coated iron oxide nanoparticle. The peptide linked dextran coated iron oxide nanoparticle can be used to bind to the formalin-fixed and paraffin-embedded tumor surgical specimens, and the method of present invention can be used to evaluate the efficacy of peptide-targeted chemotherapy for treatment of cancer patients. | 2015-04-23 |
20150111227 | PEPTIDE SPECIFIC FOR OVARIAN CANCER AND APPLICATIONS - The present invention provides a novel peptide with high specificity for recognition of ovarian cancer. The peptide is selected using M13 phage display library and epithelial-enrich-conjugated magnetic beads. Only a small amount of the peptide is required to establish over 80% binding activity to ovarian cancer comparing to low binding activities to other cancers, showing high specificity for ovarian cancer. Thus the peptide of the present invention can be effective in early detection of ovarian cancer. | 2015-04-23 |
20150111228 | OPTICAL BIOSENSOR - The present disclosure provides an optical biosensor including a complex including a porous support and an enzyme supported inside the pores of the porous support and a method for preparing the same. Since a dye does not flow but is concentrated in one place, the optical biosensor of the present disclosure has remarkably improved sensitivity. In addition, it is possible to carry out quantitative determination and qualitative analysis since color intensity increases with time. | 2015-04-23 |
20150111229 | Method for Searching for Inhibitor of Odor Cause By Furaneol - It is intended to identify a substance inhibiting an odor caused by 2,5-dimethyl-4-hydroxy-3(2H)-furanone. The present invention provides a method for searching for an inhibitor of an odor caused by 2,5-dimethyl-4-hydroxy-3(2H)-furanone, comprising: adding a test substance and 2,5-dimethyl-4-hydroxy-3(2H)-furanone to an olfactory receptor OR5K1 or a polypeptide having at least 80% amino acid sequence identity thereto; measuring the response of the olfactory receptor or the polypeptide to 2,5-dimethyl-4-hydroxy-3(2H)-furanone; and identifying a test substance inhibiting the response of the olfactory receptor or the polypeptide, on the basis of the measured response. | 2015-04-23 |
20150111230 | METHOD FOR EVALUATION OF PRESENCE OF OR RISK OF COLON TUMORS - The disclosed methods are used to predict or assess colon tumor status in a patient. They can be used to determine nature of tumor, recurrence, or patient response to treatments. Some embodiments of the methods include generating a report for clinical management. The methodology provided herein is intended to detect technical variations and to allow for data normalization and enhance signal detection and build predictive proteins profiles of disease status and response. | 2015-04-23 |
20150111231 | ANTI-sAPPbeta ANTIBODY - Provided are a monoclonal antibody capable of specifically recognizing a specific region of sAPP β or a fragment of said antibody and an assay method and an assay kit using the same, in order to accurately assay sAPPβ contained in a biological sample. | 2015-04-23 |
20150111232 | Soluble B7-H1 - This document features methods of evaluating mammals by assessing expression of B7-H1 in a body fluid. | 2015-04-23 |
20150111233 | MUTATED GENES FOR THE CATALYTIC PROTEIN OF OPLOPHORUS LUCIFERASE AND USE THEREOF - Luciferases which are different from those known heretofore have been desired. A luciferase mutant comprising an amino acid sequence in which at least one amino acid selected from the group consisting of valine at the position of 44, alanine at the position of 54 and tyrosine at the position of 138 is substituted with other amino acid(s) in the amino acid sequence of SEQ ID NO: 2. | 2015-04-23 |
20150111234 | SENSOR STORAGE AND DELIVERY SYSTEM WHERE THE TEST SENSORS ARE INDIVIDUALLY FOILED AND ARRANGED IN A STACK - A portable, hand-held glucose testing device includes a housing configured to accommodate a plurality of test sensors in a stacked arrangement and having a wall with an opening defined therein. A plurality of packaged test sensors is stacked in alignment with one another within the housing. Each of the test sensors is packaged within a blister package. The blister package includes a blister package housing and a cover foil overlying a surface of the blister package housing and the test sensor. A drive slide is configured to displace one of the plurality of packaged test sensors out of alignment with other packaged test sensors. A knife mechanism is configured to pierce through the cover foil, and to engage and urge the test sensor to extend through the opening for receiving a sample. A meter contact is configured to engage the test sensor when the test sensor extends through the opening. | 2015-04-23 |
20150111235 | METHOD FOR DETERMINING CAUSE OF THE PROLONGATION OF BLOOD COAGULATION TIME - Provided is a method of accurately and easily determining a cause of the prolongation of blood coagulation time. | 2015-04-23 |
20150111236 | Method and Device for Detecting an Analyte in a Body Fluid - A method for detecting at least one analyte in at least one sample of a body fluid is disclosed. Therein, at least one test element ( | 2015-04-23 |
20150111237 | BIOTINIDASE ASSAYS - The invention relates to methods for enzymatic detection of biotinidase activity. In certain aspects, the invention provides methods for bench-based enzymatic detection of biotinidase activity. In other aspects, the invention provides methods for bench-based enzymatic detection in droplets in oil. The droplet-based methods may be performed in an automated manner using digital microfluidics technology on a droplet actuator device. The enzymatic assays for biotinidase activity may be used for newborn testing for biotinidase deficiency, and may be combined with other droplet-based enzymatic assays in a panel of tests for newborn testing. | 2015-04-23 |
20150111238 | METHOD FOR DETECTING DISEASE BIOMARKERS - There is presently provided a method of detecting a bio-marker for a genetic disease in an individual. The method involves comprising identifying the presence of a proteolytic peptide in a polypeptide fraction obtained from a cellular extract of cells from the individual. The cells are cells by the disease to be diagnosed, and the proteolytic peptide contains a mutation associated with the genetic disease or directly related to the disease condition. | 2015-04-23 |
20150111239 | MICROFLUIDIC DEVICE AND RELATED METHODS - A combinatorial microenvironment generator is configured for the generation of arbitrary, user-defined, steady-state, concentration gradients with negligible to no flow through the growth medium to perturb diffusion gradients or cellular growth. More importantly, the absolute concentrations and/or gradients can be dynamically altered upon request both spatially and temporally to impose tailored concentration fields for in-situ stimulus studies. Here, diffusion occurs via an array of ports, each of which can be an independently controlled source/sink. Together, the array of ports establishes a user-defined, 3D concentration profile. Useful methods related to this device are also provided. | 2015-04-23 |
20150111240 | IN VITRO MODEL FOR A TUMOR MICROENVIRONMENT - Methods for mimicking a tumor microenvironment in vitro are provided. The methods comprise indirectly applying a shear stress upon at least one tumor cell type plated on a surface within a cell culture container. Methods for mimicking tumor metastasis and methods for testing drugs or compounds in such systems are also provided. | 2015-04-23 |
20150111241 | INLET AND OUTLET GEOMETRIES FOR A VERTICAL THREE-STREAM MICROFLUIDIC DEVICE - An example includes an apparatus for separating cells from a fluid sample, including a plenum, defining: a centrally located top inlet, and respective side outlets disposed below the inlet and to the side of the inlet; and a bottom outlet disposed below the top inlet, to the side of the two or more outlets, wherein the plenum is configured to receive a fluid and cells suspension through the inlet, and direct it to the bottom outlet, against respective side-walls extending between the bottom outlet and the two or more side outlets, and wherein the distance between each of the two or more side outlets and the bottom outlet is selected to encourage cells to exit through the bottom outlet under a force. Lateral/vertical distance between the inlet and side outlets can provide lateral fluid travel, thereby allowing time for gravity to bias cells toward an outlet. | 2015-04-23 |
20150111242 | CELL COLLECTING APPARATUS AND METHOD OF USE - The present invention provides an apparatus and method for microdissecting a biological sample. In particular, apparatuses of the invention include a cell collecting device | 2015-04-23 |
20150111243 | WOUND DRESSING - The invention provides vesicles that may be used in diagnosing infection, especially with infection by bacteria such as | 2015-04-23 |
20150111244 | DETECTION OF MICROORGANISMS INVOLVED IN METABOLISM OF ORGANIC ENVIRONMENTAL POLLUTANTS - A method and point of use kit to determine under field conditions the presence and/or amount of a selected | 2015-04-23 |
20150111245 | GLYCOPEGYLATED FOLLICLE STIMULATING HORMONE - The invention provides conjugates between follicle stimulating hormone and PEG moieties. The conjugates are linked via an intact glycosyl linking group that is interposed between and covalently attached to the peptide and the modifying group. The conjugates are formed from both glycosylated and unglycosylated peptides by the action of a glycosyltransferase. The glycosyltransferase ligates a modified sugar moiety onto either an amino acid or glycosyl residue on the peptide. Also provided are pharmaceutical formulations including the conjugates. Methods for preparing the conjugates are also within the scope of the invention. | 2015-04-23 |
20150111246 | SITE-SPECIFIC ENZYMATIC MODIFICATION OF EXENDINS AND ANALOGS THEREOF - There is provided inter alia a method of covalently attaching an amine derivatizing agent to a glutamine of a first polypeptide. The method includes adding an amine derivatizing agent, a transglutaminase a first polypeptide which includes a glutamine, and a co-solvent to a reaction mixture. The method further includes allowing the amine derivatizing agent to react with the glutamine of the first polypeptide in the reaction mixture to form an amide bond, thereby covalently attaching the amine derivatizing agent to the glutamine of the first polypeptide. | 2015-04-23 |
20150111247 | METHOD OF DOUBLE-COATING LACTIC ACID BACTERIA - The present disclosure relates to production of double-coated lactic acid bacteria using peptides and polysaccharide. Double-coated lactic acid bacteria show improved heat-resistance, acid-resistance, bile-resistance, storage stability and excellent survival rate when reaching intestine. | 2015-04-23 |
20150111248 | IN VIVO PRODUCTION OF PROTEINS - The invention relates to compositions and methods for the preparation, manufacture and therapeutic use of polynucleotides, primary transcripts and mmRNA molecules. | 2015-04-23 |
20150111249 | Recombinant Bacterial Host Cell for Protein Expression - The present disclosure relates to a recombinant gram-negative bacterial cell comprising: a.) a mutant spr gene encoding a spr protein having a mutation at one or more amino acids selected from D133, H145, H157, N31, R62, I70, Q73, C94, S95, V98, Q99, R100, L108, Y115, V135, L136, G140, R144 and G147 and b.) a gene capable of expressing or overexpressing one or more proteins capable of facilitating protein folding, such as FkpA, Skp, SurA, PPiA and PPiD, wherein the cell has reduced Tsp protein activity compared to a wild-type cell, methods employing the cells, use of the cells in the expression of proteins in particular antibodies, such as anti Fc Rn antibodies and proteins made by the methods described herein. | 2015-04-23 |
20150111250 | METHODS AND COMPOSITIONS FOR MODULATING TUMOR CELL ACTIVITY - Antibodies which target clusterin, a protein involved in the epithelial-to-mesenchymal transition of carcinoma cells, are identified and characterized. The antibodies may be used to modulate tumour cell activity through binding to clusterin. | 2015-04-23 |
20150111251 | TNF-ALPHA BINDING PROTEINS - TNF-α binding proteins, including chimeric, CDR-grafted, and humanized antibodies that bind TNF-α are provided. Binding proteins have high affinity for TNF-α and neutralize TNF-α activity. A binding protein can be a full-length antibody or a TNF-α-binding portion thereof. Methods of making and methods of using the binding proteins are also described. The TNF-α binding proteins are useful for detecting TNF-α and for inhibiting TNF-α activity, including in a human subject suffering from a disease or disorder in which TNF-α activity is detrimental. | 2015-04-23 |
20150111252 | METHOD AND APPARATUS FOR ANTIBODY PRODUCTION AND PURIFICATION - The subject invention pertains to methods and apparatus for the production and purification of cell products, such as immunoglobulins. One aspect of the invention is an integrated cell culture and purification apparatus for the growth and maintenance of cells and the harvest and purification of cell products, such as immunoglobulins. Thus, the apparatus integrates a cell culture function with a purification function. Other aspects of the invention pertain to an automated method for producing immunogenic compositions such as vaccines. | 2015-04-23 |
20150111253 | ENZYMATIC OMEGA-OXIDATION AND OMEGA-AMINATION OF FATTY ACIDS - The invention relates to a method for oxidizing a fatty acid or an ester thereof of formula (I) H3C—(CH2)n-COOR, wherein R is selected from the group that comprises H, methyl, ethyl, propyl, and butyl, wherein n is 0 to 30, preferably 6 to 24, comprising the step of oxidizing the fatty acid or the ester thereof by contacting the fatty acid or the ester thereof with a cytochrome P450 monooxygenase of the CYP153 family in the presence of molecular oxygen and NAD(P)H and a whole-cell catalyst that expresses a recombinant cytochrome P450 monooxygenase of the CYP153 family, a recombinant alcohol dehydrogenase, a recombinant transaminase, and optionally one or more than one recombinant enzyme from the group comprising alanine dehydrogenase, ferredoxin, and ferredoxin reductase, and the use of said whole-cell catalyst to oxidize a fatty acid or an ester thereof. | 2015-04-23 |
20150111254 | METHOD FOR AEROBICALLY PRODUCING ALANINE OR A COMPOUND PRODUCED USING ALANINE - The problem addressed by the invention is solved in a first aspect by a method for producing alanine or a compound produced using alanine, having the steps of (a) providing a cell which expresses a recombinant alanine dehydrogenase or a variant thereof, (b) cultivating the cell under aerobic conditions in the presence of an inorganic nitrogen source in an aqueous phase, and (c) bringing the cell into contact with an organic phase, said cell being a prokaryotic or a lower eukaryotic cell. | 2015-04-23 |
20150111255 | PROCESS FOR MAKING CHITIN DERIVATIVES - Disclosed is a process comprising (1) forming an aqueous mixture comprising a microbial composition and solid chitin, wherein said microbial composition comprises one or more microbes that produce chitin digesting enzymes; and (2) fermenting the mixture for a time sufficient to enzymatically digest all or part of the chitin to form a fermented mixture comprising chitosan and glucosamine. In some embodiments, the chitin is derived from the biodegradation of chitin containing marine Arthropods. In other embodiments, the chitin is obtained from chitin containing fungi, filamentous fungi and yeast which is extracted via a chemical process. In yet another embodiment, the chitin is obtained by the biodegradation of chitin containing fungi, filamentous fungi, yeast and/or insects, preferably using HQE for the digestion. In some embodiments, the process is carried out with a solution that already contains chitosan and/or glucosamine such as HYTb, the aqueous fraction obtained from the biodegradation of chitin containing organisms. | 2015-04-23 |
20150111256 | Assembly of Nucleic Acid Sequences in Emulsions - Methods and compositions for synthesizing nucleic acid sequences in an emulsion are provided. | 2015-04-23 |
20150111257 | THERMAL CYCLING APPARATUS AND METHOD - A thermal cycling apparatus having a sample interfacing wall extending from a mounting wall. The sample interfacing wall can accept and apply thermal cycles to samples. An air source can direct an air stream to cool the sample. Another source can direct heated air away from the sample. | 2015-04-23 |
20150111258 | PROCESS FOR THE PRODUCTION OF ORGANIC COMPOUNDS FROM PLANT SPECIES - This invention relates to a process for the production of fermentable C5-C6 sugars from oleaginous herbaceous plants comprising the steps of: a) mechanically separating the seeds from the above-ground lignocellulose biomass and breaking-up said lignocellulose biomass; b) placing the above-ground lignocellulose biomass in contact with a basic aqueous solution so as to obtain a paste containing from 10 to 50% by weight of the said lignocellulose biomass at temperature of between 10 and 95° C. for a time of between 1 minute and 24 hours; c) separating out the paste obtained in step b) in a solid fraction containing essentially hemicellulose and cellulose; in a liquid fraction containing lignin and extractables; d) subjecting the said solid fraction containing essentially hemicellulose and cellulose to enzyme hydrolysis. | 2015-04-23 |
20150111259 | Method for Making High Maltose Syrup - The present teachings provide direct conversion of granular starch into a soluble sugar composition comprising a high, very high, and/or ultra high maltose content. The method involves contacting an aqueous slurry of granular starch with an enzyme composition comprising an appropriate ratio of an alpha-amylase and a maltogenic enzyme to produce a soluble starch substrate that is enzymatically converted to a sugar composition containing higher maltose. The process may use more than one temperature to achieve the same. | 2015-04-23 |
20150111260 | ALDOLASES, NUCLEIC ACIDS ENCODING THEM AND METHODS FOR MAKING AND USING THEM - This invention relates to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. In some embodiments, the invention is directed to polypeptides having aldolase activity, including pyruvate activity such as, without limitation, HMG and/or KHG aldolase activity, including thermostable and thermotolerant activity, and polynucleotides encoding these enzymes, and making and using these polynucleotides and polypeptides. The polypeptides in accordance with the invention can be used in a variety of pharmaceutical, agricultural and industrial contexts. In some embodiments, the invention provides polypeptides and biosynthetic pathways that are useful in the production of R-2-hydroxy 2-(indol-3ylmethyl)-4-keto glutaric acid (R-MP) and certain stereoisomers of monatin, such as R,R and S,R monatin, and salts thereof, as well as certain stereoisomers of monatin derivatives, such as the R,R and S,R configurations, and salts thereof. | 2015-04-23 |
20150111261 | L-THREONINE-PRODUCING ESCHERICHIA COLI AND METHOD FOR PRODUCING L-THREONINE USING THE SAME - An L-threonine-producing | 2015-04-23 |
20150111262 | METHODS AND MATERIALS FOR PRODUCING FIVE CARBON BUILDING BLOCKS FROM PROLINE - This document describes biochemical pathways for producing glutaric acid, 5-aminopentanoic acid, 5-hydroxypentanoic acid, cadaverine or 1,5-pentanediol by forming one or two terminal functional groups, comprised of carboxyl, amine or hydroxyl group, in a C5 backbone substrate such as D-proline. | 2015-04-23 |
20150111263 | METHODS AND PRODUCTS FOR GENERATING OILS - Compositions and methods for enhancing secretion of oils from oil producing cells are described herein. In particular, secretion of oils is enhanced by treating the cells with herbicides. The oils generated according to the invention may be useful, for example, in the production of biofuels. | 2015-04-23 |
20150111264 | Method for Producing Lipid - Provided is a method for producing a lipid, by which lipid including a high ratio of medium-chain fatty acids can be produced efficiently. | 2015-04-23 |
20150111265 | MICROBIAL CONVERSION OF METHANE - This invention relates to a process for producing lipids and amino acids from a gaseous substrate comprising methane and oxygen. The process uses a culture of a methanotrophic microorganism in a liquid nutrient medium. The methanotrophic microorganism can be a | 2015-04-23 |
20150111266 | CARBON CAPTURE IN FERMENTATION - The invention provides processes and methods for utilization of carbon dioxide (CO | 2015-04-23 |
20150111267 | METHOD OF INITIATING ACETIC FERMENTATION UNDER INDUSTIRAL CONDITIONS - The subject of the present invention is a method of initiating acetic fermentation in a production fermenter using a starter culture cultured in a pilot fermenter which constitutes about 1-3% of the working volume of the production fermenter, and the production fermenter is inoculated in a continuous fashion with acetic fermentation bacteria cultured in the pilot fermenter. | 2015-04-23 |
20150111268 | ENZYME USED IN BIOSYNTHESIS OF 1, 4-BDO AND SCREENING METHOD OF THE SAME - Recombinant butyraldehyde dehydrogenases (Blds) with improved production of 1,4-BDO, as well as recombinant microorganisms comprising polynucleotides encoding the recombinant Blds, and methods of producing 1,4-BDO by using the recombinant microorganisms. | 2015-04-23 |
20150111269 | KETOL-ACID REDUCTOISOMERASE USING NADH - Methods for the evolution of NADPH specific ketol-acid reductoisomerase enzymes to acquire NADH specificity are provided. Specific mutant ketol-acid reductoisomerase enzymes isolated from | 2015-04-23 |
20150111270 | METHOD FOR PRODUCING SUGAR AND ETHANOL BY SELECTIVE FERMENTATION - A method for producing sugar and ethanol including the steps of: heating and cleaning a sugar liquid derived from a plant; fermenting the cleaned liquid which has been adjusted to suitable temperature to selectively convert sugar components other than sucrose in the cleaned liquid into ethanol; and concentrating the obtained fermented liquid. | 2015-04-23 |
20150111271 | MULTIPLE TANK HIGH SOLIDS ANAEROBIC DIGESTER - A multi-stage anaerobic digester is designed to treat a high solids, stackable feedstock. The system may also receive a pumpable feedstock such as a slurry or sludge. In a first stage, the digestate circulates in one direction around a raceway such that the digestate may pass a feed inlet multiple times before leaving the first tank. An optional side stream loop withdraws fibrous material from near the top of the reaceway and return digestate with chopped fibers, preferably lower and further along the raceway. An outlet from the raceway located near, but upstream of the feed inlet discharges partially digested substrate to a second stage, which is operated as a stirred tank reactor. The two stages may be provided in a single tank with an internal wall separating a ring shaped outer portion from a cylindrical inner portion. The digester may be operated in a thermophilic temperature range. | 2015-04-23 |
20150111272 | System and Method for Converting Organic Waste Into Methane and Other Useful Products - An organic waste processing system and method for producing an organic waste slurry for the production of bio-gas, transportation fuels or chemical products, wherein the system and method produces an organic waste slurry having substantially ten to fifteen percent in total solids and high levels of COD. | 2015-04-23 |
20150111273 | BIOHYDROGEN PRODUCTION METHOD AND REACTOR - A method for producing H | 2015-04-23 |
20150111274 | METHODS FOR PREPARING TE(O) FROM TE(IV) USING METAL-REDUCING BACTERIA AND IRON ION - Disclosed is a method for preparing Te(O) in a low toxic form from toxic Te(IV) using metal-reducing bacteria and iron ions. According to the present invention, extracellular tellurium nanorods can be prepared through an environmentally friendly process and are able to provide tellurium utilizable in petroleum refining, electronic devices, batteries, and sensors. | 2015-04-23 |
20150111275 | OPTICAL REGULATION OF GENE EXPRESSION IN THE RETINA - The present invention provides methods and compositions for regulating transgene expression in a subject using heat generated by electro-magnetic radiation in a target cell. The heat is generated by a thermo generator resided in the target cell upon application the electromagnetic radiation. The amount of the heat is controlled by the energy delivered by the electromagnetic radiation. | 2015-04-23 |
20150111276 | BLOOD CELL SORTING METHODS AND SYSTEMS - The invention relates to methods of isolating white blood cells (WBCs) from a sample, e.g., whole blood, using magnetic particles that specifically bind to WBCs and a series of specific steps and conditions. The methods can include one or more of decreasing the viscosity of the sample prior to WBC isolation, agitating the sample at specified frequencies, and/or using a sample container arranged such that all of the sample is placed in close proximity (e.g., within 5, 2, 1, or 0.5 mm) to the source of the magnetic field. The new methods provide for isolation of WBC preparations with high yield, purity, and viability. The methods are designed for compatibility with automation protocols for rapid processing of multiple samples. | 2015-04-23 |
20150111277 | Cell Washing Device Using a Wave - A system is disclosed for washing a selected component and removing the selected component from a wash material. The selected component may include red blood cells that are washed to remove a rejuvenating solution. The red blood cells may then be removed with various systems, such as a standing acoustic wave system from the wash solution. Pumps and flow restrictors that provide steady flow from pumps that generate pulsed flow are also disclosed. | 2015-04-23 |
20150111278 | Heat-Stable Carbonic Anhydrases and Their Use - The present invention relates to use of | 2015-04-23 |
20150111279 | Class I Anti-CEA Antibodies and Uses Thereof - The present invention provides compositions and methods of use of humanized, chimeric or human Class I anti-CEA antibodies or fragments thereof, preferably comprising the light chain variable region CDR sequences SASSRVSYIH (SEQ ID NO:1); GTSTLAS (SEQ ID NO:2); and QQWSYNPPT (SEQ ID NO:3); and the heavy chain variable region CDR sequences DYYMS (SEQ ID NO:4); FIANKANGHTTDYSPSVKG (SEQ ID NO:5); and DMGIRWNFDV (SEQ ID NO:6). The Class I anti-CEA antibodies or fragments are useful for treating diseases, such as cancer, wherein the diseased cells express CEACAM5 and/or CEACAM6 antigens. The Class I anti-CEA antibodies or fragments are also of use for interfering with specific processes, such as metastasis, invasiveness and/or adhesion of cancer cells, or for enhancing sensitivity of cancer cells to cytotoxic agents and have favorable effects on the survival of subjects with cancer. | 2015-04-23 |
20150111280 | NOVEL GLUCOSE DEHYDROGENASE - The present invention provides a novel glucose dehydrogenase that has excellent substrate specificity, specific activity, thermal stability, and the like, and that is suitable for use in SMBG sensors. The present invention provides a purified polypeptide comprising an amino acid sequence having at least 80% identity to the sequence of SEQ ID NO: 1 and having glucose dehydrogenase activity. | 2015-04-23 |
20150111281 | Production of Porphyrin-Containing Polypeptides in the Presence of Formate - The present compositions and methods relate to the production of polypeptides in submerged culture, and specifically the large-scale production of porphyrin-containing polypeptides, e.g. catalase, by microbial fermentation in the presence of formate. | 2015-04-23 |
20150111282 | HEXON ISOLATED FROM SIMIAN ADENOVIRUS SEROTYPE 19, HYPERVARIABLE REGION THEREOF AND CHIMERIC ADENOVIRUS USING THE SAME - Novel hexon isolated from simian adenovirus serotype 19 encoded in the polynucleotide defined as SEQ ID NO: 3, hepervariable region thereof, chimeric adenovirus comprising the same, and therapeutic use thereof provides a solution to the problem of safety and effective systemic treatment for developing gene therapeutic agents using adenovirus. | 2015-04-23 |
20150111283 | CULTURE MEDIUM, METHOD FOR CULTURING LISTERIA, AND METHOD FOR DETECTING LISTERIA - There are disclosed a culture media and culture supplements for cultivating microorganisms, including | 2015-04-23 |
20150111284 | METHOD FOR THE ARTIFICIAL AGING OF FABRICS AND READY-MADE GARMENTS - A method for the artificial aging of fabrics by applying an enzyme compound without using an aqueous bath. | 2015-04-23 |
20150111285 | DETERGENT FORMULATION FOR DISHWASHING MACHINE - The objective of the present invention is in the field of cleaning agent in particular detergents. In particular, it relates to a novel detergent formulation for an automatic dishwashing. The formulation provides excellent cleaning and finishing; it is environmentally friendlier than traditional compositions and allows for a more energy efficient automatic dishwashing process. | 2015-04-23 |
20150111286 | Method and System for Reducing Foam Formation in Slurry Pits of Swine Rearing Facilities with Slatted Floors - The present invention relates to a method and a system for reducing foam formation in slurry pits of swine rearing facilities with slatted floors. | 2015-04-23 |
20150111287 | PORTABLE DEVICE FOR DETECTING MOLECULE(S) - An optical assembly | 2015-04-23 |
20150111288 | SYSTEM AND METHOD INCLUDING ANALYTICAL UNITS - Systems and methods for processing and analyzing samples are disclosed. The system may process samples, such as biological fluids, using assay cartridges which can be processed at different processing locations. In some cases, the system can be used for PCR processing. The different processing locations may include a preparation location where samples can be prepared and an analysis location where samples can be analyzed. To assist with the preparation of samples, the system may also include a number of processing stations which may include processing lanes. During the analysis of samples, in some cases, thermal cycler modules and an appropriate optical detection system can be used to detect the presence or absence of certain nucleic acid sequences in the samples. The system can be used to accurately and rapidly process samples. | 2015-04-23 |
20150111289 | FLUID INTERFACE CARTRIDGE FOR A MICROFLUIDIC CHIP - An interface cartridge for a microfluidic chip, with microfluidic process channels and fluidic connection holes at opposed ends of the process channels, provides ancillary fluid structure, including fluid flow channels and input and/or waste wells, which mix and/or convey reaction fluids to the fluidic connection holes and into the process channels of the microfluidic chip. | 2015-04-23 |
20150111290 | OPTICAL DETECTOR - Disclosed herein is an optical detector at least including: a first substrate in which a plurality of wells are formed; a second substrate in which a heating section is provided to heat the wells; a third substrate in which a plurality of photoirradiation sections are provided in alignment with the wells; and a fourth substrate in which a plurality of photodetection sections are provided in alignment with the wells. | 2015-04-23 |
20150111291 | CELL DIVISION TRACKING APPARATUS AND METHOD OF THE SAME, AND NON-TRANSITORY COMPUTER READABLE STORAGE MEDIUM TO STORE A CELL DIVISION TRACKING PROGRAM - A cell division tracking apparatus includes a control unit, a search range setting unit, a daughter cell judgment unit. The control unit tracks division processes of the cells based on a cell image group composed of the cell images. The search range setting unit sets a search range to search for daughter cell regions corresponding to daughter cells resulting from a division of a mother cell based on the mother cell region detected by the mother cell detection unit. The daughter cell judgment unit judges whether the cell regions are the daughter cell regions based on a region in which regions of the cell overlap the search range regarding the cell images collected at and after a detection of the mother cell region. | 2015-04-23 |
20150111292 | PROCESSES AND SYSTEMS FOR DISCHARGING AMINE BYPRODUCTS FORMED IN AN AMINE-BASED SOLVENT - Embodiments of a process for discharging amine byproducts formed in an amine-based solvent are provided. The process comprises the steps of contacting the amine-based solvent with flue gas comprising carbon dioxide, oxygen, nitrogen, NO | 2015-04-23 |
20150111293 | CANCER CELL-TRAPPING METAL FILTER, CANCER CELL-TRAPPING METAL FILTER SHEET, CANCER CELL-TRAPPING DEVICE, AND MANUFACTURING METHODS THEREFOR - Provided are a cancer cell-trapping metal filter which has a high opening ratio, a cancer cell-trapping metal filter sheet, a cancer cell-trapping device using the cancer cell-trapping filter, and manufacturing methods therefor. | 2015-04-23 |
20150111294 | METHODS AND COMPOSITIONS FOR GENERATING AN IMMUNE RESPONSE BY INDUCING CD40 AND PATTERN RECOGNITION RECEPTOR ADAPTERS - Provided are methods for activating an antigen-presenting cell and eliciting an immune response by inducing an inducible pattern recognition receptor adapter, or adapter fragment, and CD40 activity. Also provided are nucleic acid compositions comprising sequences coding for chimeric proteins that include an inducible CD40 peptide and an inducible pattern recognition receptor adapter or adapter fragment. | 2015-04-23 |