14th week of 2022 patent applcation highlights part 22 |
Patent application number | Title | Published |
20220106536 | Thermally Conductive Lubricant - A method of lubricating a bearing, a bearing, and a lubricant with high thermal conductivity including a base oil, a polymeric thickener, and thermally conductive powder particles. The lubricant is, at atmospheric pressure, liquid above a transition temperature and a gel below said transition temperature. | 2022-04-07 |
20220106537 | REJUVENATION AND/OR EXTENSION OF THE LIFETIME OF FRICTIONAL PERFORMANCE IN TRANSMISSION FLUIDS - A booster additive concentrate may advantageously contain: (a) an anti-wear mixture of two or more phosphite/phosphate compounds and one or more ether/thioether compounds; (b) an ashless dispersant; (c) a calcium-containing detergent, such as an overbased calcium phenate; (d) ≥2 friction modifiers, at least one of which comprises a polyalkylene polyamine succinimide derivative; (e) optionally a corrosion inhibitor; and (f) a lubricating oil basestock. Based on these additive components, the booster additive concentrate may exhibit specific contents of B/Ca/P and may contain minimal or substantially no additional antioxidants. Lubricant compositions can be made from the booster additive concentrates and a fresh/used lubricant oil composition “diluent,” which can rejuvenate the diluent. Such lubricant compositions can have advantageous anti-shudder durability (ASD) lifetimes and other frictional properties. In particular, such concentrates/compositions can offer superior lubrication when used in vehicles with continuously variable transmissions (CVTs). | 2022-04-07 |
20220106538 | REFRIGERATOR OIL AND REFRIGERATOR WORKING FLUID COMPOSITION - One aspect of the present invention is a refrigerating machine oil comprising a base oil, a dithiophosphoric acid ester, and a phosphonate ester. | 2022-04-07 |
20220106539 | WASH REAGENT CONTAINING ALKOXYLATED FATTY ALCOHOL AND METHODS OF PRODUCTION AND USE THEREOF - Wash reagents for use with a hematology analyzer are disclosed. The wash reagents are capable of removing buildups from a vacuum shuttle chamber (VSC) of a unified fluid circuit (UFC) of the hematology analyzer without substantially damaging the UFC. The wash reagents contain at least one non-ionic surfactant that includes an ethoxylated fatty alcohol group. Also disclosed are kits containing the wash reagents (or at least the non-ionic surfactant component thereof), as well as methods of using the wash reagents to clean at least a portion of an analyzer. | 2022-04-07 |
20220106540 | CARE PRODUCT COMPOSITIONS HAVING IMPROVED OLFACTORY PROPERTIES - The present invention relates to a composition comprising at least one composition for home care, and at least one solid microcapsule having an average diameter of between 1 μm and 30 μm, comprising a core consisting of a composition C | 2022-04-07 |
20220106541 | Microelectronic Device Cleaning Composition - A composition for cleaning a microelectronic device substrate is provided. The composition is useful for cleaning in-process microelectronic device substrates possessing exposed cobalt, molybdenum, copper, molybdenum, tungsten, and dielectric surfaces. Also provided is a method for cleaning such devices and a kit comprising one or more of the components of the composition. | 2022-04-07 |
20220106542 | Post CMP Cleaning Compositions - In general, the invention provides high pH cleaning compositions for dielectric surfaces such as PETEOS, SiO | 2022-04-07 |
20220106543 | WATER-SOLUBLE UNIT DOSE ARTICLE COMPRISING A FIRST NON-IONIC SURFACTANT AND A SECOND NON-IONIC SURFACTANT - A water-soluble unit dose article containing a non-soap anionic surfactant, a non-ionic surfactant and a method of use thereof. | 2022-04-07 |
20220106544 | METHOD OF MAKING CONCENTRATE FOR BEVERAGES - One embodiment of the present invention is a nested fermentation method for making a beverage, such as an alcoholic or non-alcoholic beer, a wine, or the like, the method comprising the steps of: creating a fermented wort solution, wherein creating the fermented wort solution comprises performing a first fermentation process on a first wort; creating a distilled wort, wherein creating the distilled wort comprises removing an initial portion of alcohol and an initial portion of water from the fermented wort solution; and creating a concentrated fermented wort solution, wherein creating the concentrated fermented wort solution comprises adding additional fermentation ingredients to the distilled wort and performing a second fermentation process on the additional fermentation ingredients to the distilled wort. | 2022-04-07 |
20220106545 | Method of Turning Food Waste into Wine - A method for preparing an alcoholic fruit wine from food waste is described. The method includes: inspecting and selecting raw fruit, boiling the raw fruit, crushing the raw fruit, transferring a crushed raw fruit pulp to a fermentation vessel, inoculating the crushed raw fruit pulp with a yeast to allow an initial fermentation to proceed within the fermentation vessel, adjusting a dissolved sugar content based on specific gravity to produce the alcoholic fruit wine having an alcohol by volume (ABV) in a range between about 7% to about 20%, separating fermented wine liquid from a solid fruit residue, allowing the fermented wine liquid to undergo a secondary fermentation in a new fermentation vessel to create an alcoholic fruit wine, inspecting the alcoholic fruit wine, and bottling the alcoholic fruit wine. | 2022-04-07 |
20220106546 | ADVANCED TISSUE ENGINEERING SYSTEM - The invention is an automated advanced tissue engineering system that comprises a housing in which one or more tissue engineering modules are accommodated together with a central microprocessor that controls functioning of the tissue engineering modules. In one embodiment, the tissue engineering module comprises a housing supporting one or more bioreactor chamber assemblies and a fluid reservoir operationally engageable with the housing. The bioreactor chamber assemblies may be selected depending on the end product option desired and may include, for example, a cell therapy bioreactor chamber, a single implant bioreactor chamber and a multiple (mosaic) implant bioreactor chamber. | 2022-04-07 |
20220106547 | COMPOSITIONS AND METHODS OF USING PARTIAL GEL LAYERS IN A MICROFLUIDIC DEVICE - The present invention relates to the use of gels for cell cultures, including but not limited to microfluidic devices and transwell devices, for culturing cells, such as organ cells, e.g. airway cells, intestinal cells, etc., and co-culturing cells, (e.g. parenchymal cells and endothelial cells, etc). As one example, the use of gels results in improved lung cell cultures, such as when using transwells and microfluidic devices, (e.g. for culturing healthy airway epithelial cells, culturing diseased airway epithelial cells, e.g., CF epithelial cells that are ciliated). The present invention relates to fluidic devices, methods and systems for use with gel layers within a microfluidic device. In particular, a partial gel layer is disposed within a microchannel of a microfluidic device. For example, a partial gel layer has a thickness ranging between approximately 20-100 μm. A dilute partial gel layer of less than 100 μm may be formed from a polymer solution of 0.5 mg/ml. A cell-permeable partial gel layer having a thickness ranging between approximately 20-50 μm may be formed from a polymer solution of 1-3 mg/ml. A partial gel layer may be formed by a hydrodynamic shearing technique. Such thin gel layers can support a variety of cell cultures, including but not limited to single cells, cell populations, cell layers, differentiated cell layers, and/or primary tissues. The present invention is related to the field of imaging and image processing. In particular, the invention is related to imaging that supports the determination of cell membrane cilia beating frequency. For example, methods described herein encompass cilia beat frequency in the context of membrane region and/or distances between regions. Alternatively, the methods described here encompass cilia beat synchrony and correlation of beat frequency between cell membrane regions. | 2022-04-07 |
20220106548 | HIGH-THROUGHPUT ACOUSTOFLUIDIC FABRICATION OF CELL SPEROIDS - Disclosed is an apparatus, such as an acoustofluidic device, for high-throughput fabrication of multicellular spheroids. The device has a chamber substrate having at least one cell assembly channel; an acoustic transducer; and, a coupling layer disposed between the chamber substrate and the acoustic transducer. | 2022-04-07 |
20220106549 | CELL CULTURE CASSETTES AND INCUBATOR - The invention relates to cassettes for culturing cells. The invention also relates to analytical and preparative incubators for housing the cassettes. The invention also relates to methods of using the cassettes, for example, to culture cells wherein the processes of feeding and passaging cells are automated. | 2022-04-07 |
20220106550 | CELL PROCESSING UNIT, CELL PROCESSING SYSTEM AND METHODS OF USE THEREOF - The disclosure relates to a cell processing unit for cell and gene therapy manufacture comprising a housing defining an enclosure into which a cell processing platform can be mounted, a platform mounting bracket within the housing and configured and arranged to receive and retain a cell processing platform, a drive apparatus configured and arranged to operatively engage and act upon a cell processing platform to move same with respect to the platform mounting bracket, and an actuator configured and arranged to exert a force on a container mounted into the cell processing platform to expel a contents from the container. | 2022-04-07 |
20220106551 | CELL CULTURE SUBSTRATE - A cell culture substrate having surface S including cell culture part, wherein the cell culture part includes non-cell-adhesive part, and cell-adhesive part extending continuously or intermittently along periphery P of the non-cell-adhesive part and surrounding the non-cell-adhesive part. The present disclosure also relates to a kit for cell culture comprising this cell culture substrate. | 2022-04-07 |
20220106552 | INCUBATOR WITH ORBITAL SHAKER - It is described an orbital incubator shaker, which comprises an incubator housing ( | 2022-04-07 |
20220106553 | MICROFLUIDIC SORTING USING HIGH GRADIENT MAGNETIC FIELDS - Microfluidic devices are described that include a microfluidic channel, a first array of one or more magnets above the microfluidic channel, each magnet in the first array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the first array, and a second array of one or more magnets beneath the microfluidic channel, each magnet in the second array having a magnetic pole orientation opposite to a magnetic pole orientation of an adjacent magnet in the second array. The first array is aligned with respect to the second array such that magnetic fields emitted by the first array and second array generate a magnetic flux gradient profile extending through the channel. An absolute value of the profile includes a first maximum and a second maximum that bound a local minimum. The local minimum is located within the microfluidic channel or less than 5 mm away from a wall of the microfluidic channel. Methods of using the new devices are also described. | 2022-04-07 |
20220106554 | METHOD OF USING TRACK ETCHED MEMBRANES FOR THE FILTRATION OF BIOLOGICAL FLUIDS - Track-etched membranes for filtration are provided. Filtration methods utilizing such membranes and cell culture methods are also provided. | 2022-04-07 |
20220106555 | SYSTEMS AND METHODS FOR MEDICAL DEVICE CONTROL - Systems, devices, and methods of the present disclosure assist with management of tubes and hoses during surgical procedures. The systems, devices, and methods provide for the proper opening and closing of tubes to facilitate performance of steps in a surgical procedure. Systems, devices, and methods of the present disclosure control fluid delivery to and from a medical device, including devices for tissue processing and cleaning. | 2022-04-07 |
20220106556 | Antifouling Composition and Process for Production Thereof - This invention concerns a method for preparing a bacterial supernatant comprising culturing a cell of | 2022-04-07 |
20220106557 | Methods for culturing filamentous fungi in fermentation media - Methods for culturing filamentous fungi, in which the filamentous fungi are grown in a colloid of air and a fermentation medium, are provided. The methods result in more rapid and prolific growth of the filamentous fungus than has been achieved by previous methods. Biomats produced by the methods and air-medium colloids for use in the methods are also provided. | 2022-04-07 |
20220106558 | METHODS FOR CULTURING FILAMENTOUS FUNGI IN FERMENTATION MEDIA - Methods for culturing filamentous fungi, in which the filamentous fungi are grown in a colloid of air and a fermentation medium, are provided. The methods result in more rapid and prolific growth of the filamentous fungus than has been achieved by previous methods. Biomats produced by the methods and air-medium colloids for use in the methods are also provided. | 2022-04-07 |
20220106559 | NOVEL KAZACHSTANIA TURICENSIS CAU Y1706 AND COMPOSITION USING SAME - The present invention relates to a novel | 2022-04-07 |
20220106560 | SYSTEM AND METHOD FOR SORTING PARTICLES - A multi-channel system for classifying particles in a mixture of particles according to one or more characteristics including a common source of electromagnetic radiation for producing a beam of electromagnetic radiation and a beam splitter for producing multiple beams of electromagnetic radiation for directing multiple beams of electromagnetic radiation to each interrogation location associated with each flow channel of the multi-channel system. | 2022-04-07 |
20220106561 | NERVE BUNDLE AND PRODUCTION METHOD OF NERVE BUNDLE | 2022-04-07 |
20220106562 | DIFFERENTIATED AND NONDIFFERENTIATED MSC COMPOSITIONS AND USE THEREOF - Cells with a mixed mesenchymal stem cell (MSC) and astrocyte phenotype are provided. Pharmaceutical compositions comprising these cells, extracellular vesicles from these cells as well as methods of production and methods of use are also provided. | 2022-04-07 |
20220106563 | MICROGLIA-SUFFICIENT BRAIN ORGANOIDS - A method for generating a microglia-sufficient brain organoid comprising the step of incubating primitive-like macrophage cells with a brain organoid that is between about 15 to about 30 days old in cerebral organoid medium comprising CSF-1 in a low attachment cell culture vessel to generate microglia cells. The present invention also relates to a microglia-sufficient brain organoid obtained by the method as described herein. | 2022-04-07 |
20220106564 | COMPOSITION AND KIT FOR DIFFERENTIATION OF STEM CELLS INTO NEURAL CREST STEM CELLS, INCLUDING INHIBITOR OF TGF-BETA I RECEPTOR AND BMP INHIBITOR, AND METHOD USING SAME - Provided are a composition and a kit for differentiation of stem cells into neural crest stem cells (NCSCs), each including a transforming growth factor beta (TGF-β) I receptor inhibitor and 0.001 μM to 2.5 μM of a bone morphogenetic protein (BMP) inhibitor, and a method using the same. Accordingly, stem cells may be efficiently differentiated into neural crest stem cells using the simple, low-cost composition. | 2022-04-07 |
20220106565 | MULTICELL CONJUGATES FOR ACTIVATING ANTIGEN-SPECIFIC T CELL RESPONSES - The present invention provides in vitro derived a multicell conjugate comprising an iNKT cell and a dendritic cell (DC). The invention also provides methods of making the multicell conjugate and methods of using the multicell conjugate and compositions comprising the same to treat one or more conditions associated with an antigen or methods of activating an immune response. | 2022-04-07 |
20220106566 | Antigen-Presenting Neutrophil-Derived Dendritic Cells and Methods of Use Thereof - Methods and compositions for use in generating or promoting an immune response to cancer or an infection, comprising promoting differentiation of neutrophils into dendritic cells using a combination of GMCSF and (i) an immune complex comprising an antigen and an antibody comprising an Fc region that binds to FcγRIIA or FcγRIIIB, (ii) a conjugate comprising an antigen and an anti-FcγRIIIB antibody, or (iii) an anti-FcγRIIIB antibody. | 2022-04-07 |
20220106567 | AMNIOTIC FLUID CELL-DERIVED EXTRACELLULAR MATRIX AND USES THEREOF - Disclosed is a cell-derived extracellular matrix (ECM) derived in vitro from cells isolated from amniotic fluid, and methods of use for the isolation, maintenance, and proliferation of adherent cells including stem cells, as well as for the differentiation of stem cells. | 2022-04-07 |
20220106568 | STEM CELL GENERATOR AND CONSTRUCTION METHOD THEREFOR - Disclosed are a stem cell generator, a construction method therefor and the use thereof. The stem cell generator is formed by implanting a biomaterial loaded with active substances and/or cells, or a biomaterial with an osteogenic induction capability into animal or human bodies and producing organoids upon development. | 2022-04-07 |
20220106569 | METHODS FOR PROMOTING PROLIFERATION AND PROPAGATION OF STEM CELLS - The present disclosure provides a method for proliferating or propagating undifferentiated stem cells, comprising contacting a population of stem cells with an effective amount of | 2022-04-07 |
20220106570 | DISEASE MODEL - A method for producing a disease model, including a step of introducing a cancer cell or fibroblast into a recellularized organ or tissue is provided by the present invention. | 2022-04-07 |
20220106571 | CHOROID PLEXUS ORGANOIDS AND METHODS FOR PRODUCTION THEREOF - Methods and materials relating to cultured choroid plexus organoids comprising: (a) an epithelium comprising a tight epithelial barrier; and/or (b) one or more cysts surrounded by an epithelium, plus other authentic features and markers. | 2022-04-07 |
20220106572 | BLOOD BRAIN BARRIER MODEL AND METHODS OF MAKING AND USING THE SAME - Provided herein is an in vitro model of the blood brain barrier. In some embodiments, the model includes: an endothelial cell layer, and brain tissue layer comprising neuronal cells, and optionally one or more of astrocytes, pericytes, oligodendrocytes, and microglia. In some embodiments, the model further comprises a porous membrane between said endothelial cell layer and the neuronal cell layer. A microfluidic device comprising the same and methods of use thereof are also provided. | 2022-04-07 |
20220106573 | ALTERNATE DETERGENTS FOR VIRAL INACTIVATION - Detergents for use to inactivate enveloped viruses, including detergents that are considered eco-friendly that can be used in biomanufacturing operations. | 2022-04-07 |
20220106574 | PRODUCTION OF VIRUSES IN CELL CULTURE - The present invention relates to methods of replicating viruses in vitro. In particular, the invention relates to a genetically modified population of cells, and/or a population of cells treated with an exogenous compound, wherein the cells are capable of producing more virus than cells lacking the genetic modification and/or lacking treatment with the exogenous compound. The invention also relates to methods of producing populations of such cells, as well as the use of the viruses obtained to prepare vaccine compositions. | 2022-04-07 |
20220106575 | KETOREDUCTASE POLYPEPTIDES FOR THE SYNTHESIS OF CHIRAL COMPOUNDS - The present disclosure provides engineered ketoreductase enzymes having improved properties as compared to a naturally occurring wild-type ketoreductase enzyme. Also provided are polynucleotides encoding the engineered ketoreductase enzymes, host cells capable of expressing the engineered ketoreductase enzymes, and methods of using the engineered ketoreductase enzymes to synthesize a variety of chiral compounds. | 2022-04-07 |
20220106576 | Taq DNA Polymerase Mutants for Probe qPCR - Disclosed are Taq DNA polymerase mutants which exhibit enhanced efficiency in qPCR compared to the wild type Taq DNA polymerase. Such mutants include: V62S, V64S, A70F, F73A, A77F, P253G, E255K, D257R, A259F, A271F, L288S, E289K, S357I, L361S, L376S, P382G, T385I, G418P, R419D, E421K, L461S, A472F, E497K, L498S, E524K, D551R, R556D, S679I, L789S, E189K/E507K/E742K (See Sequence Listing Guide for the mutants' amino acid and protein sequences). | 2022-04-07 |
20220106577 | MODIFIED PLANTS CONTAINING COMBINATION OF APYRASE GENES AND METHOD FOR MAKING MODIFIED PLANTS WITH COMBINATION OF APYRASE GENES - The present disclosure is directed to a modified plant cell, plant tissue, plantlet, plant part, plant, and progeny thereof containing a combination of apyrase genes, including at least one modified gene and/or at least one additional apyrase gene. The disclosure is also directed to a method of making a modified plant with a modified apyrase gene and/or an additional apyrase gene. The modified plant generally exhibits at least one improved characteristic over the plant line from which it was derived. The disclosure also relates to a recombinant DNA molecule comprising a nucleotide sequence encoding an apyrase enzyme as disclosed. | 2022-04-07 |
20220106578 | MODIFIED DNASE AND USES THEREOF - Modified DNase I protein in which one or more amino acids of a DNase I protein are modified non-cellularly, are provided. The modified DNase I protein exhibits a DNA hydrolytic activity in the presence of actin and an improved DNA hydrolytic activity compared to a homologous non-modified DNase I protein. Processes of preparing the modified DNase I protein and uses thereof in, for example, reducing a DNA content in sputum and/or in treating a disease or condition associated with excess extracellular DNA in a fluid, secretion or tissue of a subject, are also provided. | 2022-04-07 |
20220106579 | Method of Treating Glycogen Storage Disease - The disclosure relates, in general, to Glycogen Storage Disease and, in particular, to a method of treating Glycogen Storage Disease and to compounds and compositions suitable for use in such a method. | 2022-04-07 |
20220106580 | TARGETED CHONDROITINASE ABC FUSION PROTEINS AND COMPLEXES THEREOF - Described herein are targeted ChABC fusion proteins, complexes thereof, and uses thereof. The targeted ChABC fusion proteins can include a ChABC polypeptide that can be linked to a Gal-3 polypeptide. Monomer targeted ChABC fusion proteins can form homogeneous or heterogeneous complexes. The targeted ChABC fusion proteins and complexes thereof can be formulated as pharmaceutical formulations. The targeted ChABC fusion proteins, complexes thereof, and formulations thereof can be administered to a subject in need thereof. | 2022-04-07 |
20220106581 | ANTI-CD137 ANTIBODIES AND METHODS OF USE THEREOF - The instant disclosure provides antibodies that specifically bind to CD137 (e.g., human CD137) and increases CD137 function. Also provided are pharmaceutical compositions comprising these antibodies, nucleic acids encoding these antibodies, expression vectors and host cells for making these antibodies, and methods of treating a subject using these antibodies. | 2022-04-07 |
20220106582 | EXOSOMAL NUCLEIC ACID EXTRACTION METHOD - An exosomal nucleic acid extraction method is provided. A substrate is provided, on which antibodies and silicon nanoparticles are disposed, and the antibodies have binding specificity to exosomal surface antigens. After that, based on the binding specificity between the antibodies and the exosomal surface antigens, exosomes in a specimen are separated. Next, the nucleic acids in the separated exosomes are adsorbed by the silicon nanoparticles. | 2022-04-07 |
20220106583 | VIRUS DETECTION IN WASTEWATER - A method is provided to extract genetic material of a virus, for example the SARS-CoV-2 virus, from a wastewater sample. The wastewater sample is in the range of 0.5 to 5 mL in volume. The wastewater sample may be prefiltered, but is not separated to concentrate the virus in a solids fraction. A lysis buffer is added to the sample, followed by a waiting period of at least one minute, followed by the addition of an alcohol. 1.5-4.5 mg of magnetic beads are added per mL of wastewater sample. The magnetic beads are later separated from the sample. Genetic material is eluted from the magnetic beads, optionally at a temperature of at least 45° C. A test kit is provided for use in performing the method. | 2022-04-07 |
20220106584 | METHODS AND KITS FOR GENERATING AND SELECTING A VARIANT OF A BINDING PROTEIN WITH INCREASED BINDING AFFINITY AND/OR SPECIFICITY - Somatic hypermutation promotes affinity maturation of antibodies by targeting the cytidine deaminase AID to antibody genes, followed by antigen-based selection of matured antibodies. Given the importance of antibodies in medicine and research, developing approaches to reproduce this natural phenomenon in cell culture is of some interest. The inventors use here the CRISPR-Cas 9 based CRISPR-X approach to target AID to antibody genes carried by expression vectors in HEK 293 cells. This directed mutagenesis approach, combined with a highly sensitive antigen-associated magnetic enrichment process, allowed rapid progressive evolution of a human antibody against the Human Leucocyte Antigen A*0201 allele. Starting from a low affinity monoclonal antibody expressed on Ag-specific naïve blood circulating B cells, they obtained in approximately 6 weeks antibodies with a two log increase in affinity and which retained their specificity. The strategy for in vitro affinity maturation of antibodies is applicable to virtually any antigen. It not only allows to tap into the vast naive B cell repertoire but could also be useful when dealing with antigens that only elicit low affinity antibodies after immunization. Accordingly as defined by the claims, the present invention relates to methods and kits for generating and selecting a variant of antibody binding protein with increased binding affinity and/or specificity. | 2022-04-07 |
20220106585 | METHOD OF SEQUENCING NUCLEIC ACID WITH UNNATURAL BASE PAIRS - Disclosed is a method of sequencing a nucleic acid containing an unnatural base pair (UBP), comprising performing two or more replacement replication reactions wherein the nucleic acid is replicated using two or more intermediate of the unnatural base pair; sequencing the nucleic acid resulting from the replacement replication reactions; clustering the sequenced nucleic acid and identifying a candidate position of the unnatural base pair; determining a ratio of conversion of the intermediate to each one of a natural base pair at the candidate position of the unnatural base pair; comparing the ratio of conversion of the intermediate to a library of pre-determined conversion rate based on the sequences of one or more natural base pair adjacent to the candidate position of the unnatural base pair; wherein a substantial match of the ratio of conversion of the intermediate to a value in the library of the pre-determined conversion rate confirms the position of the unnatural base pair, thereby determining the sequence of the nucleic acid containing the unnatural base pair. Also disclosed is an apparatus for performing the method as disclosed herein. | 2022-04-07 |
20220106586 | COMPOSITIONS AND METHODS FOR LIBRARY SEQUENCING - Provided herein are compositions and methods for next generation sequencing using universal polynucleotide adapters. Further provided are universal adapters which provide for a large number of samples to be sequenced in parallel. Further provided are methods for high-throughput testing of samples for disease testing. | 2022-04-07 |
20220106587 | METHODS OF SEQUENCING NUCLEIC ACIDS IN MIXTURES AND COMPOSITIONS RELATED THERETO - This disclosure relates to analyzing the end-to-end sequence and the relative distributions in heterogeneous mixtures of polynucleotides and methods and enabling reagents related thereto. In certain embodiments this method relates to the complete full length sequencing and quantitative profiling of mRNAs present in the transcriptomes of cells or tissues of, but not limited to, higher multicellular organisms that possess interrupted genes subject to complex post-transcriptional RNA processing | 2022-04-07 |
20220106588 | DNA SEQUENCING USING HYDROGEL BEADS - Systems, methods, and compositions provided herein relate to preparation of beads encapsulating long DNA fragments for high-throughput spatial indexing. Some embodiments include preparation of nucleic acid libraries within the bead, wherein the bead includes pores that allow diffusion of reagents while retaining genetic material. | 2022-04-07 |
20220106589 | METHODS AND SYSTEMS FOR MODELING OF DESIGN REPRESENTATION IN A LIBRARY OF EDITING CASSETTES - Disclosed systems and methods relate to predicting the relative representation of genomic variants in an edited cell population, based on the editing cassette design representation in an editing cassette design library used to generate the edited cell population. A library of editing cassette designs is generated, and a feature vector, or sequence embedding, is developed for each design using natural language processing techniques. The feature vector may be based upon sequence attributes and editing kinetics of each cassette design as well as attributes that describe the library context. Features may include sequence embeddings generated from a neural network, linguistic-type distances, and statistical distance summaries thereof. The feature vectors are classified using one or more machine learning models, and the classified feature vectors are used to predict the representation of each design an edited cell population. | 2022-04-07 |
20220106590 | HYBRIDIZATION METHODS AND REAGENTS - Provided herein are compositions and methods for improving hybridization reactions. Further provided herein are synthetic blocking libraries. Further provided herein are methods for designing synthetic blocking libraries, and application towards methylome analysis. | 2022-04-07 |
20220106591 | Methods and Compositions for the Single Tube Preparation of Sequencing Libraries Using Cas9 - Methods and compositions of single tube preparation of sequencing libraries from a target DNA are provided. The methods include contacting the DNA with a composition comprising Cas9 endonuclease, a first and a second guide RNAs, a ligase, and sequencing adapters, subjecting the composition to thermal cycling to cleave the DNA at the sites flanking the regions of interest by the RNA guided endonuclease, and subjecting the composition to a temperature to allow ligation of the cleaved DNA fragments including the regions of interest with the sequencing adapters to generate the sequencing libraries. | 2022-04-07 |
20220106592 | DUX4 RNA Silencing Using RNA Targeting CRISPR-CAS13b - RNA interference-based products and methods for inhibiting the expression of the double homeobox 4 (DUX4) gene on human chromosome 4q35 are disclosed. The disclosure includes the Cas13 protein silencing of RNA, wherein Cas13 is specifically targeted to a DUX4 region of interest using a sequence- specific guide RNA (gRNA). Recombinant adeno-associated viruses of the disclosure deliver DNAs encoding inhibitory gRNAs that are constructed with Cas13 direct repeats to knock down the expression of DUX4. The methods have application in the treatment of muscular dystrophies including, but not limited to, facioscapulohumeral muscular dystrophy (FSHD), and other disorders associated with elevated DUX4 expression, including cancer. | 2022-04-07 |
20220106593 | MULTI-TARGETED SINGLE ENTITY CONJUGATES - The present invention relates, in general to, compounds, compositions and methods useful for modulating gene expression of multiple target nucleic acids by a single chemical entity. | 2022-04-07 |
20220106594 | Methods for Treating Oculopharyngeal Muscular Dystrophy (OPMD) - The present disclosure relates to methods of administering a gene therapy construct comprising RNA interference (RNAi) reagents, such as short hairpin microRNA (shmiR), in combination with PABPN1 replacement reagents, such as polynucleotides which encode functional PABPN1 protein which are not targeted by the RNAi reagents, for treatment of oculopharyngeal muscular dystrophy (OPMD) in individuals suffering from OPMD or which are predisposed thereto. In certain aspects the method comprises direct injection to a subject's pharyngeal muscles. | 2022-04-07 |
20220106595 | ANTIBACTERIAL ANTISENSE AGENTS - The invention relates to improved ANTISENSE agents for the treatment of gram-negative bacterial infections. Compounds of the invention utilise an Antibiotic-Assisted Translocation; AAT′ platform to improve influx into bacterial cells through enhanced permeability, providing improved intracellular exposure of the ANTISENSE AGENT and superior treatment of the infection. | 2022-04-07 |
20220106596 | A METHOD OF GENE EDITING - Disclosed herein are methods of editing a gene in a cell that involve contacting the cell with a replication fork modulator, as well as edited cells and their methods of use. | 2022-04-07 |
20220106597 | Methods For Preventing Titration Of Bimolecular Templated Assembly Reactions By Structurally-Determined Differential Hybridizations - The present disclosure provides nucleic acid molecules, and kits comprising the same, for producing templated assembly products for a cell. | 2022-04-07 |
20220106598 | HOMOLOGOUS RECOMBINATION DIRECTED GENOME EDITING IN EUKARYOTES - Disclosed herein are synthetic nucleic acids comprising a nucleic acid sequence that encodes an ANAGO that is a species-specific to a eukaryote, such as a human, and compositions comprising ANAGO, such as a human ANAGO, and donor molecules for use in homologous recombination directed targeted gene editing in the eukaryote, such as in human cells. | 2022-04-07 |
20220106599 | ANTIVIRAL AGENTS - An antiviral agent is provided, having a phosphorodiamidate morpholino oligomer with an antisense sequence to a portion of a genome of a strain of Zika virus (ZIKV). The antiviral agent finds many uses, such as in a pharmaceutical composition, a method of treating ZIKV-mediated disease, a method of preventing ZIKV-mediated disease, a method of reducing or preventing the replication of ZIKV in a host cell, a method of controlling the spread of ZIKV in donated tissue, a treated tissue sample, and in the manufacture of a medicament for the treatment or prevention or ZIKV-mediated disease. | 2022-04-07 |
20220106600 | CRISPR-CAS-RELATED METHODS, COMPOSITIONS AND COMPONENTS FOR CANCER IMMUNOTHERAPY - CRISPR/CAS-related compositions and methods for treatment of cancer. | 2022-04-07 |
20220106601 | Antisense Drug Against the Human Intercellular Adhesion Molecule 1 (ICAM-1) - The present invention relates to a specific antisense oligonucleotide which inhibits the expression of the adhesion molecule ICAM-1 in human cells. The present invention further relates to a vector containing said oligonucleotide and a host cell containing said vector or oligonucleotide and a pharmaceutical composition containing said oligonucleotide as well as uses thereof, in particular in the treatment of an inflammatory disease or condition in humans. | 2022-04-07 |
20220106602 | NUCLEIC ACID CONSTRUCT - The present invention provides a nucleic acid construct comprising: a first nucleotide sequence of interest (NOI1); a frame-slip motif or a translational readthrough motif (FSM/TRM); and a second nucleotide sequence of interest (NOI2). The invention also provides vectors and cells expressing such a construct. The invention also provides a method for modulating the relative expression of two transgenes in a nucleic acid construct which comprises the step of including a frame-slip motif or a translational readthrough motif between the two transgenes in order to reduce the expression of the downstream transgene. | 2022-04-07 |
20220106603 | ENGINEERED DYSBIOSIS-SENSING PROBIOTIC FOR CLOSTRIDIUM DIFFICILE INFECTIONS AND RECURRING INFECTIONS MANAGEMENT - The present invention relates to methods of metabolic engineering bacteria cells to produce bile salt hydrolase to inhibit the germination of | 2022-04-07 |
20220106604 | METHOD FOR INCREASING CANNABIS YIELD VIA GENE EDITING - The present invention discloses a method for increasing flower yield in | 2022-04-07 |
20220106605 | METHOD FOR IMPROVING RICE YIELD AND/OR RICE BLAST RESISTANCE AND PROTEIN USED THEREOF - The invention discloses a method for improving rice yield and a protein used thereof. The invention provides a method for cultivating the target rice, comprising the following steps of inhibiting the activity of RAY1 protein in original rice to obtain target rice; compared with the original rice, the target rice shows higher yield and/or larger grain size and/or stronger resistance to rice blast and/or higher plant height and/or longer stem internode length; the RAY1 protein is a protein composed of an amino acid sequence shown as SEQ ID No. 1 in a sequence list. The invention uses CRISPR/Cas9 technology to realize site-directed editing rice RAY1 gene, through knocking out rice RAY1 gene by frameshift mutation, the protein RAY1 is inactivated, and a new generation of rice germplasm with significantly improved yield is obtained. | 2022-04-07 |
20220106606 | TRANSGENIC PLANTS WITH ENHANCED TRAITS - This disclosure provides recombinant DNA constructs and transgenic plants having enhanced traits such as increased yield, increased nitrogen use efficiency and enhanced drought tolerance; propagules, progeny and field crops of such transgenic plants; and methods of making and using such transgenic plants. This disclosure also provides methods of producing seed from such transgenic plants, growing such seed and selecting progeny plants with enhanced traits. Also disclosed are transgenic plants with altered phenotypes which are useful for screening and selecting transgenic events for the desired enhanced trait. | 2022-04-07 |
20220106607 | GENE FOR PARTHENOGENESIS - The invention provides the nucleotide sequence and amino acid sequences of the parthenogenesis gene of | 2022-04-07 |
20220106608 | FUSOGENIC LIPID NANOPARTICLES FOR THE TARGET CELL-SPECIFIC PRODUCTION OF RAPAMYCIN INDUCIBLE THERAPEUTIC PROTEINS - Provided nucleic acid-based expression construct for the target cell-specific production of a therapeutic protein, such as a pro-apoptotic protein, within a target cell, including a target cell that is associated with aging, disease, or other condition, in particular a target cell that is a senescent cell or a cancer cell. Also provided are formulations and systems, including fusogenic lipid nanoparticle (LNP) formulations and systems, for the delivery of nucleic acid-based expression constructs as well as methods for making and using such nucleic acid-based expression constructs, formulations, and systems for reducing, preventing, and/or eliminating the growth and/or survival of a cell, such as a senescent cell and/or a cancer cell, which is associated with aging, disease, or other condition as well as methods for the treatment of aging, disease, or other conditions by the in vivo administration of a formulation, such as a fusogenic LPN formulation, comprising an expression construct for the target cell-specific production of a therapeutic protein, such as a pro-apoptotic protein, in a target cell that is associated with aging, disease, or other condition, in particular a target cell that is a senescent cell or a cancer cell. | 2022-04-07 |
20220106609 | PARAVOVIRAL VECTORS AND METHODS OF MAKING AND USE THEREOF - A recombination parvovirus vector that comprises a parvovirus capsid and a double-stranded vector genome having a sense-strand and an antisense-strand. The sense-strand comprises in the 5′ to 3′ direction: a parvovirus terminal repeat at the 5′ end a coding sequence of a gene of interest (GOI); and a parvovirus terminal repeat at the 3′ end. The vector genome further comprises a RNA destabilization/destruction domain (RDDD). | 2022-04-07 |
20220106610 | METHODS AND SYSTEMS FOR MANUFACTURING VIRAL VECTORS - Disclosed herein are methods and systems for manufacturing viral vectors (e.g., lentiviral vectors) using a static light scattering device in-line with a manufacturing process. | 2022-04-07 |
20220106611 | VECTORIZED ANTIBODIES AND USES THEREOF - Provided are recombinant adeno-associated virus (rAAV) compositions for the expression of antibodies (e.g., anti-complement component 5 (C5) antibodies) in cells, and methods of treating disorders with the same (e.g., disorders associated with C5 activity (e.g., Paroxysmal Nocturnal Hemoglobinuria)). Also provided are compositions, systems and methods for making the rAAV compositions. | 2022-04-07 |
20220106612 | ADENO-ASSOCIATED VIRUS VECTOR - Disclosed herein is a recombinant adeno-associated virus (AAV) vector comprising (a) a variant AAV2 capsid protein, wherein the variant AAV2 capsid protein comprises at least four amino acid substitutions with respect to a wild type AAV2 capsid protein; wherein the at least four amino acid substitutions are present at the following positions in an AAV2 capsid protein sequence: 457, 492, 499 and 533; and (b) a heterologous nucleic acid comprising a nucleotide sequence encoding a gene product. | 2022-04-07 |
20220106613 | NEUROD1 VECTOR - The present disclosure relates to AAV vectors, compositions, and methods related to converting glial cells to neurons by the use of a NeuroD1 coding sequence in an AAV vector. | 2022-04-07 |
20220106614 | DLX2 VECTOR - The present disclosure relates to AAV vectors, compositions, and methods related to converting glial cells to neurons by the use of a Dlx2 coding sequence in an AAV vector. | 2022-04-07 |
20220106615 | METHODS, COMPOSITIONS AND KITS FOR INCREASING GENOME EDITING EFFICIENCY - Methods of editing a target genomic region(s), methods of repairing of a DNA break via a HDR pathway, methods of inhibiting or suppressing repair of a DNA break via a NHEJ pathway, and methods of modifying expression of a gene(s) or protein(s) comprise administering to one or more cells that include one or more target genomic regions, a genome editing system and a DNA protein-kinase (DNA-PK) inhibitor disclosed herein. Kits and compositions for editing a target gene comprise a genome editing system and a DNA-PK inhibitor disclosed herein. | 2022-04-07 |
20220106616 | PRODUCTION OF CANNABINOIDS IN FILAMENTOUS FUNGI - The present invention relates to genetically modified ascomycetous filamentous fungi, particularly of the species | 2022-04-07 |
20220106617 | METHOD OF PRODUCING HIGHLY UNSATURATED FATTY ACID CONTAINING GLYCERIDES USING LIPASE HYDROLYSIS REACTION - The present invention provides a method of producing a composition containing docosahexaenoic acid as a constituent fatty acid of glycerides, comprising hydrolyzing a feedstock oil containing glycerides comprising docosahexaenoic acid as a constituent fatty acid by action of a first lipase and a second lipase, thereby increasing the proportion of docosahexaenoic acid in glyceride fractions, wherein the first lipase is at least one lipase selected from the group consisting of a lipase derived from a microorganism of the genus | 2022-04-07 |
20220106618 | BIOSYNTHETIC PRODUCTION OF GAMMA- AND DELTA-LACTONES USING CYTOCHROME P450 ENZYMES HAVING SUBTERMINAL HYDROXYLASE ACTIVITY - Provided herein are methods for making lactones comprising incubating a cellular system expressing a heterologous cytochrome P450 (CYP450) protein with a medium comprising straight chain fatty acids to produce subterminal hydroxy fatty acids, and incubating a yeast cell culture with the subterminal hydroxy fatty acids to produce lactones. | 2022-04-07 |
20220106619 | ABC TRANSPORTERS FOR THE HIGH EFFICIENCY PRODUCTION OF REBAUDIOSIDES - Provided herein are genetically modified host cells, compositions, and methods for improved production of steviol glycosides. In some embodiments, the host cell is genetically modified to comprise a heterologous nucleic acid expression cassette that expresses an ABC-transporter capable of transporting steviol glycosides to the extracellular space or to the luminal space of an intracellular organelle. In some embodiments, the host cell further comprises one or more heterologous nucleotide sequence encoding further enzymes of a pathway capable of producing one or more steviol glycosides in the host cell. The host cells, compositions, and methods described herein provide an efficient route for the heterologous production of steviol glycosides, including but not limited to, rebaudioside D and rebaudioside M. | 2022-04-07 |
20220106620 | DESIGNING CUSTOMIZED PROTEIN-SPECIFIC-BUFFER SYSTEM - The present invention is related to the field of protein chemistry. In particular, mixed buffer compositions are formulated that allow an accurate identification of agent-induced changes in protein melting point temperatures. Such buffer compositions provide for methods that determine the specific effects of exogenous agents on protein stability, cryoprotective effects and/or protein quality control (e.g., synthesis and/or extraction purity validations). | 2022-04-07 |
20220106621 | SYSTEM AND METHOD FOR DETECTING A TARGET ENZYME - The present invention relates to a system for detecting a target enzyme in a sample, the system including a substrate, a first enzyme immobilized on the substrate via a first linker, and a second enzyme immobilized on the substrate via a second linker, wherein the first linker is cleavable by the target enzyme or the second enzyme to release the first enzyme for cleaving the second linker, thereby releasing the second enzyme; and a method for detecting a target enzyme in a sample including applying the sample to said system. | 2022-04-07 |
20220106622 | METHOD FOR PATHOGEN IDENTIFICATION - The present invention relates to a method for detecting a pathogen in cellular lysate by measuring pathogen-specific enzyme activity. The method comprises contacting the cellular lysate with a substrate the pathogen of interest recognizes and modifies, and obtaining a measurable, recordable, signal. The method may comprise detection of SARS-CoV viruses using the activity of SARS PLpro enzyme in tongue scrape lysate as a readout. | 2022-04-07 |
20220106623 | SPATIAL MAPPING OF CELLS AND CELL TYPES IN COMPLEX TISSUES - Systems and methods to tag cells in a tissue sample with spatial identifiers, so that spatial reconstruction of cell locations within a tissue can be achieved after tissue disaggregation are described. The systems and methods allow the control or directing of specific spatial identifiers spatially within or throughout a tissue to individual cells or to a small population of cells so that cell position can be determined by computational deconvolution of the spatial identifiers after tissue disaggregation. The systems and methods can be combined with single cell expression analysis to correlate cell types with cell location within a structure, such as a tumor. | 2022-04-07 |
20220106624 | ASYMMETRIC TEMPLATES AND ASYMMETRIC METHOD OF NUCLEIC ACID SEQUENCING - The invention is a novel method of making and using a template for nucleic acid sequencing. The templates include circular and linear templates with symmetric and asymmetric adaptors. The methods include utilizing the templates in an asymmetric fashion. | 2022-04-07 |
20220106625 | COMPOSITIONS AND METHODS FOR ISOLATION OF CELL-FREE DNA - Provided herein are compositions and methods for isolating cell-free nucleic acid, e.g., cell-free DNA, from a sample. In particular embodiments, provided herein are compositions and methods using anti-dsDNA antibodies for isolating cell-free DNA from a sample, and for providing a sample of isolated cell-free DNA, e.g., for a nucleic acid assay. In particular embodiments, the technology relates to providing cell-free DNA from a maternal sample that is enriched for fetal cell-free fetal DNA. | 2022-04-07 |
20220106626 | NOVEL METHOD FOR PERFORMING PCR REACTION USING COMPREHENSIVE PCR REACTION SYSTEM - Provided are a method for performing a PCR reaction using a PCR reaction system, a PCR reaction system, as well as a composition, a freeze-dried powder, and a diluent. The PCR reaction method includes: subjecting the piston to the first and second movement processings, to subject the diluent entering the injection chamber, the pyrolysis freeze-dried powder, and the sample to a first mixing processing; subjecting the first mixture to a pyrolysis processing; subjecting the piston to the third and fourth movement processings, to subject the pyrolysis liquid entering the injection chamber and the remaining diluent to a second mixing processing; subjecting the piston to the fifth and sixth movement processings, to allow the second mixture to enter the injection chamber and undergone a third mixing processing with the freeze-dried powder of the reverse transcriptase and the PCR raw materials; and subjecting the third mixture to a PCR amplification processing. | 2022-04-07 |
20220106627 | METHODS OF DIAGNOSING TUBERCULOSIS AND DIFFERENTIATING BETWEEN ACTIVE AND LATENT TUBERCULOSIS - Compositions and methods for detecting | 2022-04-07 |
20220106628 | METHODS AND COMPOSITIONS FOR CAPPING NUCLEIC ACIDS - A method for identifying a nucleic acid template that include (a) providing a plurality of primer-template hybrids, wherein a first hybrid of the plurality includes a first template hybridized to a first primer, and wherein a second hybrid of the plurality includes a second template hybridized to a second primer, the second primer having a ternary complex inhibitor moiety at the 3′ end; (b) delivering polymerases and nucleotides to the plurality, whereby the first hybrid binds a polymerase and nucleotide to form a stabilized ternary complex and whereby the second hybrid does not bind a polymerase and nucleotide to form a stabilized ternary complex; and (c) detecting the stabilized ternary complex to identify the first template. | 2022-04-07 |
20220106629 | METHOD OF TRANSLOCATING NUCLEIC ACIDS THROUGH NANOPORES - The invention provides methods for analyzing polynucleotides using nanopores that allow passage of single stranded polynucleotides but not double stranded polynucleotides. In accordance with some embodiments, a double-stranded product is produced that comprises a labeled strand with a single stranded tail or overhang. The double stranded product is exposed to one or more nanopores in the presence of an electric field across the one or more nanopores such that the single stranded tail may be captured and the labeled strand translocated by unzipping from the double stranded product. The ionic composition of the reaction mixture and electric field strength are selected so that nucleotides translocate a nanopore at a rate of less than 1000 nucleotides per second. | 2022-04-07 |
20220106630 | Nanopore Platform for DNA/RNA Oligo Detection Using an Osmium Tagged Complementary Probe - Provided for herein is a method for detecting the presence of a nucleic acid target molecule in a biological sample. In certain aspects, the method comprises contacting a test sample that comprises (i) a biological sample comprising a nucleic acid target molecule and (ii) an osmylated single-stranded oligonucleotide probe comprising at least one pyrimidine residue covalently bonded to a substituted or unsubstituted Osmium tetroxide (OsO | 2022-04-07 |
20220106631 | BARCODING IN DROPLETS FOR CELL-CELL INTERACTION AND SECRETED PROTEIN DETECTION AND ANALYSIS - The present invention provides compositions, systems, and methods for barcoding cells, beads, and secreted proteins in discrete entities (e.g. droplets) to allow sequencing data from such components that are separated during processing to be associated via the common barcodes. In some embodiments, the barcodes are tethered to the cell surface via a lipid, cholesterol, or antibody, or are attached to a surface molecule that moves from one cell to another via trogocytosis. In certain embodiments, such methods allow cell-cell interactions or secreted proteins in the discrete entity to be monitored. | 2022-04-07 |
20220106632 | SPATIAL ANALYSIS TO DETECT SEQUENCE VARIANTS - Provided herein are methods of detecting an analyte of interest to interrogate spatial gene expression in a sample. | 2022-04-07 |
20220106633 | SPATIAL TRANSCRIPTOMICS FOR ANTIGEN-RECEPTORS - Provided herein are methods, compositions, and kits for the detection of immune cell clonotypes and immune cell analytes within a biological sample. | 2022-04-07 |
20220106634 | DRY REAGENT, REAGENT KIT, METHOD OF PRODUCING DRY REAGENT, ANALYTICAL METHOD AND NUCLEIC ACID AMPLIFICATION METHOD - According to one embodiment, a dry reagent includes an insoluble fiber and a dried material containing a biological material, which is retained in the fiber. | 2022-04-07 |
20220106635 | METHOD FOR MEASURING TELOMERE ASSOCIATED VARIABLES AND USES THEREOF FOR THE DIAGNOSIS AND/OR PROGNOSIS OF TELOMERIC-ASSOCIATED DISEASES - The invention provided methods in vitro for diagnosis and/or prognosis of a clinical outcome in a subject with a telomere-associated disease comprising determining specific telomere associated variables (TAVs) of a test sample cell from the subject, wherein the value of the TAVs obtained for the test sample compared to a control sample is indicative of the telomerase-associated disease and/or the clinical outcome of the subject. | 2022-04-07 |