| 14th week of 2009 patent applcation highlights part 38 |
| Patent application number | Title | Published |
|---|
| 20090087829 | PRODUCTION OF MONATIN ENANTIOMERS - Methods for preferentially hydrolyzing one stereoisomer of an isoxazoline diester over another, as well as an enzyme for facilitating the preferential hydrolysis are provided. Also provided are methods for providing mixtures of (RR) and (RS) monatin as well as (SS) and (SR) monatin, which methods can include the step of stereoselectively hydrolyzing an isoxazoline diester. | 2009-04-02 |
| 20090087830 | SYSTEMS AND METHODS FOR HARVESTING, STORING, AND IMPLANTING HAIR GRAFTS - A system and method for harvesting, storing, and implanting biological units, in particular hair follicular units (FUs). The system is particularly useful to facilitate hair transplant procedures. FUs are harvested from a body surface, either attached to a patient or in a strip of removed tissue, and shuttled into a cartridge having a plurality of receptacles. The receptacles are open in a distal direction toward a removal tool, but a cover over the proximal ends of the receptacles prevents the FUs from continuing out of the cartridge. The cover is made of a permissible medium, which may be fluid permeable and/or puncturable. One way to shuttle the FUs is to provide a pressure differential, such as by applying suction to the proximal end of a receptacle. The shuttle subsystem may be incorporated within an overall automated or robotic system, or the shuttle subsystem may form part of a semi-automated or even manual apparatus. | 2009-04-02 |
| 20090087831 | METHODS FOR EVALUATING A BACTERIOPHAGE PREPARATION - A quality assurance/quality control paradigm for bacteriophage is provided. | 2009-04-02 |
| 20090087832 | Nucleic acids and new polypeptides associated with and/or overlapping with hepatitis C virus core gene products - DNA encoding core+1 polypeptides of hepatitis C virus (HCV), nucleotides encoding the polypeptides, and methods for using the nucleotides and the encoded polypeptides are disclosed. | 2009-04-02 |
| 20090087833 | ADIPOGENIC ADENOVIRUSES AS A BIOMARKER FOR DISEASE - This invention relates to the relationship between infection with an adipogenic adenovirus, such as adenovirus-36, and obesity-related disease. In particular, this invention relates to assaying a subject to determine the adipogenic adenovirus infection status and then determining the subject's predisposition to developing an obesity-related disease based on the adipogenic adenovirus infection status. | 2009-04-02 |
| 20090087834 | Method for characterising polynucleotides - The method of the invention is used to identify specific characteristics of a target polynucleotide in a sample, and comprises the steps of: i) attaching to one end of each target polynucleotide in the sample a polynucleotide signal sequence that is specific for the characteristic under study; ii) contacting the target polynucleotides with a molecule that interacts with the target polynucleotide if the characteristic is present; iii) attaching a polynucleotide adapter sequence to those targets polynucleotides that interact with the molecule of step (ii), the adapter being attached at the end of the target polynucleotide opposite that at which the signal sequence is attached; iv) carrying out a polynucleotide amplification reaction on those target polynucleotides that comprise both the adapter and signal sequence, optionally repeating steps (i) to (iv) for other characteristics; and (v) identifying which signal sequences are present on the amplified products, and in which order, to thereby determine the characteristics of each target polynucleotide. | 2009-04-02 |
| 20090087835 | Method of Identifying Hairpin DNA Probes by Partial Fold Analysis - Method of identifying molecular beacons in which a secondary structure prediction algorithm is employed to identify oligonucleotide sequences within a target gene having the requisite hairpin structure. Isolated oligonucleotides, molecular beacons prepared from those oligonucleotides, and their use are also disclosed. | 2009-04-02 |
| 20090087836 | IL-B30 ANTIBODIES - Purified genes encoding cytokine from a mammal, reagents related thereto including purified proteins, specific antibodies, and nucleic acids encoding this molecule are provided. Methods of using said reagents and diagnostic kits are also provided. | 2009-04-02 |
| 20090087837 | Method Of Detecting Streptococcus Pneumoniae, Primer Set For The Detection And Kit For The Detection - A method of detecting | 2009-04-02 |
| 20090087838 | ANALYTE DETECTION USING AUTOCATALYTIC CHAIN REACTIONS - Compositions and methods for detecting the presence of analytes employing autocatalytic chain reactions (ACR) having super linear kinetics for amplification of signal are disclosed. | 2009-04-02 |
| 20090087839 | LONG DISTANCE POLYMERASE CHAIN REACTION-BASED ASSAY FOR DETECTING CHROMOSOMAL REARRANGEMENTS - Methods are presented for determining the presence of an inversion in the factor VIII gene which cause hemophilia A. The methods encompass long distance, multiplex PCR (including overlapping PCR). The use of deaza-dGTP, high levels of DNA polymerases and high levels of DMSO aid in successfully performing the PCR. The use of a novel technique called subcycling PCR can also be applied as part of the methods. The technique allows for the determination of whether a person is homozygous or hemizygous for the inversion and has hemophilia A or whether a person is heterozygous for the inversion and is a carrier. The technique of long distance, multiplex PCR including use of deaza-dGTP, high levels of DNA polymerases and high levels of DMSO are applicable to the determination of the presence of other gross chromosomal aberrations such as deletions/inversions, translocations and inversions. The use of subcycling PCR can achieve efficient and more even amplification than normal two or three temperature PCR and is applicable to long distance, multiplex PCR. | 2009-04-02 |
| 20090087840 | Combined extension and ligation for nucleic acid assembly - Certain aspects of the present invention provide methods for assembling nucleic acid molecules. Some embodiments involve analyzing nucleic acid sequences and determining appropriate assembly strategies based on the presence or absence of sequence features that are known or predicted to interfere with extension-based and/or ligation-based assembly techniques. Aspects of the invention also provide kits, compositions, devices, and systems for assembling synthetic nucleic acids using polymerase-based techniques, ligase-based techniques, or combinations thereof. | 2009-04-02 |
| 20090087841 | METHODS AND COMPOSITIONS FOR DETERMINING RESISTANCE OF HIV-1 TO PROTEASE INHIBITORS - This invention relates to methods for determining resistance of HIV-I viruses to protease inhibitors (PIs) based on the viral genotypes. The methods generally comprise detecting, in a gene encoding protease of the HIV-I, the presence of a mutation in at least one of codon 22, 69, 74, or 83 alone or in combination with one or more mutations at certain other codons, or, in a gene encoding gag of the HIV-I, the present of a mutation in at least one of codon 418 or 482 alone or in combination with one or more mutations at certain other codons. Combinations of mutations associated with resistance to PIs are also disclosed. | 2009-04-02 |
| 20090087842 | Presynaptic protein cast - The present invention enabled the detection and quantification of CAST, which is localized to synapses and tightly bound to the cytomatrix, and of the mRNA encoding the CAST. Furthermore, it was revealed that CAST functions as a protein scaffold for localizing RIM1 to synapses, contributing as a molecular basis for active zone formation. | 2009-04-02 |
| 20090087843 | MOLECULAR MARKERS - The present invention relates to genetic sequences exhibiting differential expression patterns in cancer tissue relative to normal tissue. The identification of such sequences permits their use as molecular markers for cancer, as early indicators of cancer progression and/or as predictive markers for a propensity or likelihood of a cancer to develop. The present invention relates particularly to genetic sequences exhibiting expression patterns up-regulated in cervical cells or associated with pre-, early- or late-onset cervical cancer relative to normal cervical cells, such sequences serving as biomarkers. The biomarkers of the present invention provide targets for the development of therapeutic protocols for the treatment or prophylaxis of cervical or related cancer. Such therapeutic protocols are directed to inhibiting expression of the marker or inhibiting the expression product of the marker. The invention is further directed to a method for identifying molecular markers which are useful indicators of cervical cancer and/or its progression. | 2009-04-02 |
| 20090087844 | METHODS AND COMPOSITIONS FOR CORRELATING GENETIC MARKERS WITH CARDIOVASCULAR DISEASE - The present invention provides methods of identifying a subject having an increased or decreased risk of developing cardiovascular disease, comprising:
| 2009-04-02 |
| 20090087845 | Genetic Markers Of True Low Birth Weight - The present application provides for a method of diagnosing TLBW via measurement of expression of various TLBW-related genes. The present application also provides kits for the diagnosis of TLBW in a subject. The present application also provides a method for determining the basis for appropriate therapy for a subject suffering from TLBW. | 2009-04-02 |
| 20090087846 | Method for Detecting Large Mutations and Duplications Using Control Amplification Comparisons to Paralogous Genes - Methods for querying biological samples to detect genetic mutations, particularly insertions and deletions, by co-amplification of a gene of interest in conjunction with a paralogous gene. When the gene of interest and the corresponding paralogous gene are selected from the CYP450 family, the resulting ratios may predict how a particular patient metabolizes certain prescription drugs. | 2009-04-02 |
| 20090087847 | DETERMINING A NUCLEIC ACID SEQUENCE IMBALANCE - Methods, systems, and apparatus are provided for determining whether a nucleic acid sequence imbalance exists within a biological sample. One or more cutoff values for determining an imbalance of, for example, the ratio of the two sequences (or sets of sequences) are chosen. The cutoff value may be determined based at least in part on the percentage of fetal DNA in a sample, such as maternal plasma, containing a background of maternal nucleic acid sequences. The cutoff value may also be determined based on an average concentration of a sequence per reaction. In one aspect, the cutoff value is determined from a proportion of informative wells that are estimated to contain a particular nucleic acid sequence, where the proportion is determined based on the above-mentioned percentage and/or average concentration. The cutoff value may be determined using many different types of methods, such as sequential probability ratio testing (SPRT). | 2009-04-02 |
| 20090087848 | DETERMINING SEGMENTAL ANEUSOMY IN LARGE TARGET ARRAYS USING A COMPUTER SYSTEM - A method and/or system for making determinations regarding samples from biologic sources including statistical methods for making meaning grouping of observed data and/or for pre-selecting endpoints. | 2009-04-02 |
| 20090087849 | NUCLEIC ACID-BASED METHODS AND COMPOSITIONS FOR THE DETECTION OF OVARIAN CANCER - Methods and compositions for identifying ovarian cancer in a patient sample are provided. The methods of the invention comprise detecting overexpression or underexpression of at least one nucleic acid biomarker in a body sample, wherein the biomarker is selectively overexpressed or underexpressed in ovarian cancer. The body sample may be, for example, an ovarian tissue sample. The biomarkers of the invention include any nucleic acid molecule that is selectively overexpressed in ovarian cancer, including, for example, MMP-7, PAEP, CA125, HE4, PLAUR, MUC-1, SLPI, SSP1, MSLN, SPON1, interleukin-7, folate receptor 1, claudin 3, inhibin A, inhibin BB, inhibin BA, and PAI-1. Overexpression or underexpression of a biomarker of interest is detected at the nucleic acid level using such methods as real-time PCR and various nucleic acid hybridization techniques. Kits for practicing the methods of the invention are further provided. | 2009-04-02 |
| 20090087850 | NUCLEIC ACID SEQUENCING METHODS AND SYSTEMS - Sequencing methods that use an exonuclease that comprises template dependent nucleobase binding activity are provided. Related compositions and sequencing systems are also provided. | 2009-04-02 |
| 20090087851 | Lineage-Restricted Neuronal Precursors - A self-renewing restricted stem cell population has been identified in developing (embryonic day 13.5) spinal cords that can differentiate into multiple neuronal phenotypes, but cannot differentiate into glial phenotypes. This neuronal-restricted precursor (NRP) expresses highly polysialated or embryonic neural cell adhesion molecule (E-NCAM) and is morphologically distinct from neuroepithelial stem cells (NEP cells) and spinal glial progenitors derived from embryonic day 10.5 spinal cord. NRP cells self renew over multiple passages in the presence of fibroblast growth factor (FGF) and neurotrophin 3 (NT-3) and express a characteristic subset of neuronal epitopes. When cultured in the presence of RA and the absence of FGF, NRP cells differentiate into GABAergic, glutaminergic, and cholinergic immunoreactive neurons. NRP cells can also be generated from multipotent NEP cells cultured from embryonic day 10.5 neural tubes. Clonal analysis shows that E-NCAM immunoreactive NRP cells arise from an NEP progenitor cell that generates other restricted CNS precursors. The NEP-derived E-NCAM immunoreactive cells undergo self renewal in defined medium and differentiate into multiple neuronal phenotypes in mass and clonal culture. Thus, a direct lineal relationship exists between multipotential NEP cells and more restricted neuronal precursor cells present in vivo at embryonic day 13.5 in the spinal cord. Methods for treating neurological diseases are also disclosed. | 2009-04-02 |
| 20090087852 | MAMMALIAN SELENOPROTEIN DIFFERENTIALLY EXPRESSED IN TUMOR CELLS - A 15 kDa selenium-containing protein (“selenoprotein”) is disclosed. The protein is shown to be differentially expressed in cancer cells, such as prostate cancer cells. There is a correlation between the presence of a polymorphism at nucleotide positions 811 and 1125 of the 15 kDa selenoprotein gene, and the presence of cancer. This polymorphism is more prevalent in the African American population. The determination of an individual's genotype may be used as an indicator of the need for dietary selenium supplementation to inhibit tumor development. Compositions including the isolated protein, specific binding agents that recognize the protein, as well as underlying nucleic acid sequences are presented, as are methods of using such compositions. | 2009-04-02 |
| 20090087853 | HERBICIDE TOLERANT COTTON PLANTS AND METHODS FOR PRODUCING AND IDENTIFYING SAME - The invention pertains to transgenic cotton plants, plant material and seeds, characterized by harboring a specific transformation event, particularly by the presence of a gene encoding a protein that confers herbicide tolerance, at a specific location in the cotton genome. The cotton plants of the invention combine the herbicide tolerant phenotype with optimal agronomic performance. | 2009-04-02 |
| 20090087854 | METHODS FOR GENETIC ANALYSIS - Methods of treating an individual exhibiting a medical condition are disclosed. The methods involve determining a score of an individual based on the individual's genotypic information, comparing the score to at least one threshold value, wherein the result of the comparison is indicative of a beneficial response to a treatment, and providing a suitable treatment to the individual. | 2009-04-02 |
| 20090087855 | MARKERS OF ALTERATIONS IN THE Y CHROMOSOME AND USES THEREFOR - Novel sequence tagged sites (STSs), probes and primers useful, e.g., for detecting the presence or absence of an STS in a sample, and methods of using these STSs, probes and primers, e.g., in methods of detecting alterations in the Y chromosome are disclosed. These compositions are also useful in methods of diagnosing or aiding in the diagnosis and/or cause of reduced sperm count and in methods of predicting or aiding in the prediction of the likelihood of success of infertility treatments. | 2009-04-02 |
| 20090087856 | METHOD FOR ADMINISTERING ANTICOAGULATION THERAPY - The present invention provides a method for use in treating a patient with an anticoagulant to optimize drug therapy and/or to prevent an adverse drug response. More particularly, the present invention relates to a method and system for use in treating a patient with Coumadin® or a substance containing warfarin. Methods of the present invention utilize variables that include the patient's CYP4F2 genotype. | 2009-04-02 |
| 20090087857 | Molecular Beacons for DNA-Photography - The present invention refers to a detection method for analytes using the principle of black-and-white photography and to reagent kits for performing the method, furthermore applied this new technology to detect a biologically relevant sequence in the nanomolar range (femtomoles) in an application circumventing the necessity of a PCR. There are still numerous ways to optimize this methodology that is suitable for a large variety of applications in the genomic diagnostics and proteomics areas. | 2009-04-02 |
| 20090087858 | Two-color Real-time/End-point Quantitation of MicroRNAs (miRNAs) - The present invention is directed to methods, reagents, kits, and compositions for detecting target polynucleotide sequences, especially small target polynucleotides such as miRNAs, between two samples. A pair of linker probes can be employed in two different reactions to query a particular species of target polynucleotide. A pair of detector probes, a single forward primer specific for the target polynucleotide, and a reverse primer can be employed in an amplification reaction to query the difference in expression level of the target polynucleotide between the two samples. In some embodiments a plurality of small miRNAs are queried with a plurality of linker probes. The plurality of queried miRNAs can then be decoded in a plurality of amplification reactions. | 2009-04-02 |
| 20090087859 | Microfluidic device for detecting soluble molecules - The present disclosure provides a microfluidic device that is compatible with standard centrifuges and may be used for point-of-care disease detection. The detection methodology may be based on microELISA. | 2009-04-02 |
| 20090087860 | HIGHLY SENSITIVE SYSTEM AND METHODS FOR ANALYSIS OF PROSTATE SPECIFIC ANTIGEN (PSA) - The invention described herein provides methods, compositions, kits, and systems for the sensitive detection of prostate specific antigen. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of prostate specific antigen. | 2009-04-02 |
| 20090087861 | Chimeric Tymovirus-like Particles and Process Thereof - The present disclosure relates to chimeric tymovirus-like particles (TVLPs) comprising a fusion protein that further comprises of a first protein that is a truncated tymovirus coat protein and a second protein. These chimeric TVLPs are useful as antigens. The present disclosure provides a highly efficient means for differentiating Foot and Mouth Disease Virus (FMDV) infected animals from vaccinated animals. The present disclosure further provides a process for the production of chimeric TVLPs and a diagnostic kit for the determination of specific antibodies of FMDV to differentiate FMDV infected from vaccinated animals. The present disclosure also provides the use of the chimeric TVLPs for diagnostic purposes. | 2009-04-02 |
| 20090087862 | Chemical Sensing Device - The present invention relates to chemical sensing and in particular to a method for detecting an analyte in a sample of whole blood. The method comprise, in summary, the steps of: exposing the sample to a transducer having a tethered reagent; introducing a labelled reagent; irradiating the sample with a series of pulses of electromagnetic radiation at a wavelength of 600 run or above; and transducing and detecting the electrical signal and the time delay between each pulse. The label on the labelled reagent absorbs the electromagnetic radiation at a level which is at least equal to the absorption of the sample of whole blood at the wavelength of the electromagnetic radiation used. | 2009-04-02 |
| 20090087863 | Method For Detecting Polypeptide Toxic to Diabrotica Insects - Disclosed is a novel Lepidopteran- and Coleopteran-active δ-endotoxin polypeptide, and compositions comprising the polypeptide, peptide fragments thereof, and antibodies specific therefor. Also disclosed are vectors, transformed host cells, and transgenic plants that comprise nucleic acid segments encoding the polypeptide. Also disclosed are methods of identifying related polypeptides and polynucleotides, methods of making and using transgenic cells comprising the novel sequences of the invention, as well as methods for controlling an insect population, such as the Western Corn Rootworm and Colorado potato beetle, and for conferring to a plant population resistance to the target insect species. | 2009-04-02 |
| 20090087864 | Procedure for the determination of the activity of the protease which activates factor VII from protein solutions - A procedure for the determination of the activity of the protease which activates blood clotting factor VII from protein solutions is describes, in which
| 2009-04-02 |
| 20090087865 | Monoclonal Antibodies to Tacrolimus and Immunoassays Methods for Tacrolimus | 2009-04-02 |
| 20090087866 | HUMAN T2R RECEPTORS FOR RANITIDINE, STRYCHNINE AND DENATONIUM AND RELATED ASSAYS FOR IDENTIFYING HUMAN BITTER TASTE MODULATORS - The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter ligands, i.e., acetaminophen, ranitidine, strychnine and denatonium. The present invention further relates to the use of these receptors in assays for identifying ligands that modulate the activation of these taste receptors and which may be used as additives in foods, beverages and medicinals for modifying (blocking) T2R-associated bitter taste. | 2009-04-02 |
| 20090087867 | BIOSENSOR - The invention provides a biosensor comprising a microbe-binding aptamer(s) in the substrate recognition element. It is possible to obtain a stabilized biosensor wherein the detection sensitivity for target microbe (target bacterium) is not impaired depending on the storage condition or measuring sample, and target bacterium in a body fluid can be directly measured by insertion of the substrate recognition element of the biosensor. | 2009-04-02 |
| 20090087868 | Neural Proteins as Biomarkers for Nervous System Injury and Other Neural Disorders - The present invention identifies biomarkers that are diagnostic of nerve cell injury and/or neuronal disorders. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of nerve injury, the cell(s) involved in the injury, and the subcellular localization of the injury. | 2009-04-02 |
| 20090087869 | SANDWICH IMMUNOASSAY AND METHOD OF DETECTING AN ANTIGEN BY USING THE SAME - The invention provides a method of sandwich immunoassay comprising steps of, (a) forming a complex of an antigen and a first antibody by contacting the antigen with the first antibody which recognizes the antigen and which is labeled by a detectable labeling substance; and (b) fixing the complex formed in the step (a) to a solid phase by using a second antibody which recognizes the antigen and which is capable of binding to the solid phase, as well as a method for detecting an antigen in an analyte by using a method of sandwich immunoassay. | 2009-04-02 |
| 20090087870 | HEMATOLOGICAL ASSAY AND KIT - The present invention is directed to a kit and a method for a fast and direct determination of the coagulation potential of a sample of blood or plasma utilising a thrombin substrate. The kit comprises at least one activator of the plasmatic coagulation system and a thrombin substrate with a K | 2009-04-02 |
| 20090087871 | Method for Identifying PDE5-Modulators - The invention relates to a novel polypeptide containing the GAF | 2009-04-02 |
| 20090087872 | ASSAY FOR IN VITRO TESTING OF ENZYME CATALYTIC ACTIVITY - The invention provides a method of evaluating metabolism-based drug interactions. The method involves selecting time points for the determination of the inactivation rate constant of a time-dependent enzyme inhibitor based on the results of a multi-time point IC50 test. Advantageously, with the subject invention, the determination and use of the multi-time point IC50 test provides an indication of the inactivation rate of a test compound and eliminates trial and error tests associated with the selection of appropriate assay conditions for the second assay conducted to determine the inactivation rate constant of the test compound. | 2009-04-02 |
| 20090087873 | SHARPLY RESOLVING LABELED PROTEIN MOLECULAR WEIGHT STANDARDS - Pre-labeled protein standards useful in electrophoresis that have sharp, consistent separation characteristics that are substantially the same as those of their unlabeled counterparts are provided. The invention provides pre-labeled protein standard sets that include a plurality of labeled proteins that are labeled on a first amino acid, in which side reactions of the label with amino acids not targeted for labeling are reduced. | 2009-04-02 |
| 20090087874 | GLUCOSE DEHYDROGENASE AND PRODUCTION THEREOF - The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) from | 2009-04-02 |
| 20090087875 | Alzheimer's related proteins and methods of use - Disclosed is a method for identifying substances that alter the interaction of a presenilin protein with a presenilin-binding protein, including contacting at least the interacting domain of a presenilin protein to a presenilin-binding protein in the presence of a test substance, and measuring the interaction of the presenilin protein and the presenilin-binding protein. Also disclosed is method for identifying substances that modulate the nuclear translocation of an armadillo protein, including providing a culture of cells that express the armadillo protein and a mutant presenilin protein, or a functional fragment thereof that binds an armadillo protein; contacting the culture with a test substance; inducing nuclear translocation of the armadillo protein in the cells; and measuring levels of nuclear armadillo protein as compared to a control as an indication of modulatory activity of the test substance. Further disclosed is method for screening individuals for presenilin alleles associated with Alzheimer's Disease or related disorders, including obtaining cells from an individual to be tested for Alzheimer's Disease or a related disorder; inducing nuclear translocation of an armadillo protein in the cells; and measuring levels of the nuclear armadillo protein as compared to a control as an indication of the presence or absence of presenilin alleles associated with Alzheimer's Disease or a related disorder. | 2009-04-02 |
| 20090087876 | METHODS AND COMPOSITIONS USEFUL FOR MODULATING DRUG-INDUCED IMPAIRMENT - The present invention provides isolated nucleic acids, polypeptides, oligonucleotides, vectors, host cells, antibodies, compositions, and kits relating to happyhour. Also provided are methods of screening for agents capable of modulating happyhour activity. | 2009-04-02 |
| 20090087877 | INFECTIVITY ASSAY - There is provided a method for infecting a target cell with a TSE agent, comprising: i) contacting said target cell with a membrane preparation, wherein the membrane preparation comprises the TSE agent and a donor membrane; and ii) infecting said target cell with the TSE agent. There is also provided a contiguous membrane, comprising a donor membrane and a membrane containing a TSE agent, wherein the TSE agent is selected from the group consisting of CJD, vCJD, familial CJD (e.g. FFI or CSS), iatrogenic CJD, BSE, ovine BSE, and CWD. | 2009-04-02 |
| 20090087878 | Nucleic acid molecules associated with plants - Polynucleotides useful for improvement of plants are provided. In particular, polynucleotide sequences are provided from plant sources. Polypeptides encoded by the polynucleotide sequences are also provided. The disclosed polynucleotides and polypeptides find use in production of transgenic plants to produce plants having improved properties. | 2009-04-02 |
| 20090087879 | Method for the production of a lysate used for cell-free protein biosynthesis - The invention relates to a method for producing a lysate used for cell-free protein biosynthesis, comprising the following steps: a) a genomic sequence in an organism, which codes for an essential translation product that reduces the yield of cell-free protein biosynthesis, is replaced by the foreign DNA located under a suitable regulatory element, said foreign DNA coding for the essential translation product that additionally contains a marker sequence; b) the organism cloned according to step a) is cultivated; c) the organisms from the culture obtained in step b) are lysed; and d) the essential translation product is eliminated by means of a separation process that is selective for the marker sequence. Also disclosed are said lysate and the use thereof. | 2009-04-02 |
| 20090087880 | PROCESS AND GENES FOR EXPRESSION ANDOVEREXPRESSION OF ACTIVE [FeFe] HYDROGENASES - A process for expression of active [FeFe]-hydrogenase in a host organism that does not contain either the structural gene(s) for [FeFe]-hydrogenases and/or homologues for the maturation genes HydE, HydF and HyG, comprising: cloning the structural hydrogenase gene(s) and/or the maturation genes HydE, HydF and HydG from an organisms that contains these genes into expression plasmids; transferring the plasmids into an organism that lacks a native [FeFe]-hydrogenase or that has a disrupted [FeFe]-hydrogenase and culturing it aerobically; and inducing anaerobiosis to provide [FeFe] hydrogenase biosynthesis and H?2#191 production. | 2009-04-02 |
| 20090087881 | Oncokinase fusion polypeptides associated with hyperproliferative and related disorders, nucleic acids encoding the same and methods for detecting and identifying the same - Oncokinase fusion polypeptides associated with hyperproliferative disorders and the polynucleotides encoding for such fusion polypeptides are provided. The fusion polypeptides have a C-terminal tyrosine kinase domain fused to an N-terminal domain that is not normally fused to the C-terminal tyrosine kinase domain and they possess constitutively activated tyrosine kinase activity. Also provided are methods for detecting and identifying the fusion polypeptides and polynucleotides and methods of diagnosing disease conditions associated with the fusion polypeptides and polynucleotides. In addition, screening assays for identifying agents useful for treating disease conditions associated with such fusion polypeptides and polynucleotides are provided. Furthermore, methods of treating disease conditions associated with the presence of the fusion polypeptides are provided. | 2009-04-02 |
| 20090087882 | Protein secretion in eukaryotic cells - The invention relates to the use of a glucosidase II mutation to increase protein secretion in yeast cells. The invention relates further to the use of yeast cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant α-1,2-mannosidase gene and/or a recombinant N-acetylglucosaminyl-transferase gene, as a host for protein secretion. | 2009-04-02 |
| 20090087883 | Novel expression vector with enhanced gene expression capacity and method for using the same - The present invention provides a novel expression vector which comprises a gene of interest, a nuclear anchoring element, and at least one inverted repeat element, preferably two inverted repeat elements. The expression vector is an episomal vector capable of transfecting a mammalian cell. The present invention further provides a method for enhancing gene expression by transfecting the expression vector to a mammalian cell, preferably a human cell. | 2009-04-02 |
| 20090087884 | Microfluidic nucleic acid amplification and separation - Microfluidic devices designed for assaying biochemical molecules are disclosed. The microfluidic devices are capable of assaying nucleic acids for identification of nucleic acid species. The microfluidic devices are adapted to carry out an amplification of the nucleic acid and subsequent separation of amplified nucleic acid species. Also disclosed is a method for amplifying and separating a nucleic acid sample on a microfluidic device. | 2009-04-02 |
| 20090087885 | Process For Preparing Optically Active Amino Acids Using a Whole-Cell Catalyst - The present invention relates to a process for preparing, in particular, enantiomerically enriched L-α-amino acids, in particular those of the general formula (I). In this connection, the process according to the invention uses 2-ketocarboxylic acids which are converted into the desired products using a whole-cell catalyst which comprises an amino acid dehydrogenase and a cofactor-regenerating enzyme. | 2009-04-02 |
| 20090087886 | METHOD FOR PRODUCING AN L-AMINO ACID USING A BACTERIUM OF THE ENTEROBACTERIACEAE FAMILY WITH ATTENUATED EXPRESSION OF THE sfmACDFH-fimZ CLUSTER OR THE fimZ GENE - The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to genus | 2009-04-02 |
| 20090087887 | METHOD FOR PRODUCING L-AMINO ACID - An L-amino acid is produced by culturing an L-amino acid-producing bacterium which belongs to the | 2009-04-02 |
| 20090087888 | PRODUCTION OF MONATIN STEREOISOMERS - Methods and materials for the production of the high intensity sweetener, monatin, in stereoisomerically-pure or stereoisomerically-enriched form are disclosed. For example, methods using stereoisoselective hydrolysis and separation of a monatin-derived lactone ester are disclosed. | 2009-04-02 |
| 20090087889 | Methods and compositions for growth hydrocarbons in botryococcus sp. - Bottom dwelling colonies of | 2009-04-02 |
| 20090087890 | METHODS OF PRODUCING ORGANIC PRODUCTS WITH PHOTOSYNTHETIC ORGANISMS AND PRODUCTS AND COMPOSITIONS THEREOF - Provided herein are compositions and methods for producing products by photosynthetic organisms. The photosynthetic organisms can be genetically modified to effect production, expression, or both, of products. The methods and compositions are particularly useful in the petrochemical industry. | 2009-04-02 |
| 20090087891 | METHODS AND DEVICE FOR PRODUCING HYDROGEN FROM BIOMASS - Disclosed herein are methods of producing molecular hydrogen, where the methods comprise contacting a solution comprising urea with a urease to produce ammonia, and contacting the ammonia with a first catalyst to produce a first gaseous mixture comprising molecular hydrogen. | 2009-04-02 |
| 20090087892 | METHODS FOR PRODUCING MUTANT MICROBES USEFUL FOR PRECIOUS METAL AND BIOENERGY PRODUCTION - A mutant microbe that generates trace amounts of gold on silver, and uses of the mutant microbe for producing and recovering precious metals and for producing biofuels and oil products from biomass and sedimentary organic matter are described. According to an exemplary embodiment, the mutant microbe is produced by placing metallic silver in an aqueous solution, and adding a species of | 2009-04-02 |
| 20090087893 | Modified Expandase Enzyme and its use - The present invention relates to a mutant penicillin expandase which comprises an amino acid substitution at one or more residue positions corresponding to those of a wild-type expandase selected from the group consisting of threonine at position 42, isoleucine at position 50, histidine at position 57, threonine at position 67, valine at position 133, threonine at position 143, proline at position 145, glycine at position 148, phenyl alanine at position 152, proline at position 196, alanine at position 240, cysteine at position 281, Serine at position 309, provided that the amino acid substitution at the residue position of cysteine at position 281 is not tyrosine. | 2009-04-02 |
| 20090087894 | Method for improving enzymatic activity of glycosyltransferases - The present invention provides an inexpensive and simple method which allows efficient glycosylation with glycosyltransferases derived from microorganisms of the Vibrionaceae family when compared to conventional enzymatic reaction systems. | 2009-04-02 |
| 20090087895 | EXPRESSION VECTORS FOR PRODUCING TAG-CLEAVABLE FUSION PROTEINS IN MULTIPLE EXPRESSION SYSTEMS - This invention features a kit containing multiple expression vectors for producing tag-cleavable fusion proteins in various expression systems, or for producing fusion proteins in | 2009-04-02 |
| 20090087896 | Live bacteria liquid product applicator and remote management system therefore - An applicator machine for applying a liquid carrier having live bacteria suspended therein, which are in a dormant state, to a target host comprising a pump having inlet and discharge sides, with the inlet side of the pump being in fluid communication with the liquid carrier. A first fluid conduit extends from the discharge side of the pump to an air induction nozzle which is in communication with a source of air under pressure. A flow control is imposed in the first conduit for adjusting the amount of liquid carrier passing therethrough. The pump and the air supply for the air induction nozzle are operatively connected to a power supply. The pump, when activated, causes the liquid carrier to be pumped to the air induction nozzle wherein the liquid carrier is mixed with air to create small droplets thereof for spraying onto the target host. A remote management system for the machine(s) is also provided. | 2009-04-02 |
| 20090087897 | Prevention of bacterial growth in fermentation process - A fermentation process for the production of ethanol from natural sources, such as corn, comprising introducing a fermentable sugar, an inoculant, and a stabilized chlorine dioxide into a fermentation system is disclosed. The stabilized chlorine dioxide is added preventatively to the fermentation system, at concentrations in the fermentation system of acetic acid no greater than 0.30% (weight/volume) and lactic acid no greater than 0.60% (weight/volume). The stabilized chlorine dioxide is added in an amount effective to substantially prevent growth of bacteria. | 2009-04-02 |
| 20090087898 | Methods, processes and apparatus of sequestering and environmentally coverting oxide(s) of carbon and nitrogen - The instant invention presents improved means for sequestering CO | 2009-04-02 |
| 20090087899 | Method and structure for extracting molecular species - The present invention in one embodiment provides a method for extracting molecular material including providing a probe comprising a penetration portion having a nanoscale surface for penetrating a biological compartment, a receptor present on the penetrating portion of the probe, wherein the receptor has an affinity for a target molecular material from the biological compartment; inserting the probe into the biological compartment, the receptor present on the penetrating portion of the probe engages the target molecular material; and extracting the probe and the target molecular material engaged to the inserting portion of the probe from the biological compartment. | 2009-04-02 |
| 20090087900 | Apparatus for Performing Electrodistention on Algae Cells - Two apparatuses capable of performing electroporation are disclosed. The first apparatus uses a Marx generator with a substantial change from its original waveform. The second apparatus does not use a Marx generator. | 2009-04-02 |
| 20090087901 | Analytical Test Element and Method for Blood Analyses - An analytical test element for blood analyses is provided having an application site and a microfluidic channel structure in fluid communication with the application site. The channel structure comprises at least first and second analytical channels for receiving first and second portions of a blood sample applied to the application site. The first analytical channel comprises a first analytical site to determine the total haemoglobin value (Hb) of the blood sample. The second analytical channel comprises a second analytical site to determine a glycohaemoglobin value (HbA1c) of the blood sample. | 2009-04-02 |
| 20090087902 | METHODS AND COMPOSITIONS FOR RAPIDLY DETECTING AND QUANTIFYING VIABLE LEGIONELLA - Methods and compositions detect and quantify viable | 2009-04-02 |
| 20090087903 | Temperature control device with a flexible temperature control surface - A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber. | 2009-04-02 |
| 20090087904 | Apparatus for handling and classifying microtomized tissue samples - The invention relates to an apparatus for handling microtomized tissue samples ( | 2009-04-02 |
| 20090087905 | VEGF-D polynucleotides and methods of use - VEGF-D, a new member of the PDGF family of growth factors, which among other things stimulates endothelial cell proliferation and angiogenesis and increases vascular permeability, as well as nucleotide sequences encoding it, methods for producing it, antibodies and other antagonists to it, transfected or transformed host cells for expressing it, pharmaceutical compositions containing it, and uses thereof in medical and diagnostic applications. | 2009-04-02 |
| 20090087906 | NOVEL CELL-BASED ASSAYS FOR G-PROTEIN-COUPLED RECEPTOR-MEDIATED ACTIVITIES - Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers. | 2009-04-02 |
| 20090087907 | Compositions and Methods for Growth of Pluripotent Cells - A method of propagating embryonic stem (ES) cells in an undifferentiated state, while maintaining both the pluripotency and the cells normal genotype is disclosed. The method comprises using recombinantly produced protein domains to attach human embryonic stem cells to the surface of a bioreactor. The ES cells are supplied with nutrients while they held in place by the recombinantly produced protein domains which may be chosen from Laminin G domain, Fibronectin domain 2, Fibronectin domain 3, Nidogen G2 domain, Nidogen G3 domain, Vitronectin somatomedin B domain, and Vitronectin somatomedin C terminal domain. Useful molecules are characterized by a high binding affinity for hES cells and a molecular weight of about 50 kDa±20%. | 2009-04-02 |
| 20090087908 | Methods for Stratification and Storage of Somatic Embryos - In one aspect, a method is provided for producing stratified cotyledonary conifer somatic embryos. The method comprises (a) incubating a culture comprising immature conifer somatic embryos in a culture vessel comprising a development medium having an osmolality in the range of from 300 mM/Kg to 450 mM/Kg at a temperature of from 22° C. to 25° C. for a first incubation period sufficient in length for at least a portion of the embryos to reach anatomical maturity; and (b) subjecting the embryos in the culture vessel in accordance with step (a) to a temperature of from 0° C. to 10° C. for a second incubation period of at least one week lo produce stratified cotyledonary somatic embryos. | 2009-04-02 |
| 20090087909 | Use of Trehalose in Conifer Somatic Embryogenesis to Increase Germination Vigor - In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) culturing a plurality of immature conifer somatic embryos for a first incubation period in, or on, a first development medium that comprises less than 0.1% trehalose; and (b) culturing the plurality of immature conifer somatic embryos treated in accordance with step (a) for a second incubation period in, or on, a second development medium that comprises at least 0.1% trehalose. | 2009-04-02 |
| 20090087910 | PRIMER-EXTENSION BASED METHOD FOR THE GENERATION OF siRNA/miRNA EXPRESSION VECTORS - Functional shRNA is produced from an expression vector prepared by selecting a two primer design in which the primers are less than about 50 nucleotides in length, annealing and extending the primers using primer extension, digesting the primer extension product and inserting the digestion product into a suitable vector. When the shRNA vectors are inserted into a cell, shRNA transcribed from the vectors modulates gene activity within the cell. | 2009-04-02 |
| 20090087911 | CODED OPTICAL EMISSION PARTICLES FOR SUBSURFACE USE - Tagging system and method including a plurality of particles, each particle having a miniature body and configured to provide a non-radioactive resolvable optical emission in a distinguishable pattern when selectively illuminated. The particles are set for selective release to a subsurface location. An apparatus having an elongated body configured for subsurface disposal and a chamber to house a plurality of particles therein. | 2009-04-02 |
| 20090087912 | TAGGED PARTICLES FOR DOWNHOLE APPLICATION - A tagged object includes a main body and a plurality of coded particles. Each coded particle may have a miniature body and be configured to provide a resolvable optical emission pattern when illuminate. The plurality of coded particles may be immobilized to the main body. A method for performing oilfield monitoring may include disposing of different types of tagged objects at different locations, wherein the different types of tagged objects each comprise a plurality of coded particles. Each of the coded particles may have a miniature body containing rare earth elements configured to produce a unique optical emission pattern when illuminated. The method may include allowing an event to trigger the release of one of the different types of tagged objects from one of the different locations. In addition, the method may include identifying the released tagged objects by unique optical emission patterns, in some cases in order to determining an occurrence location of the event. | 2009-04-02 |
| 20090087913 | Analysis of conjugated metabolites of alcohol consumption - A method, system, kit and uses for quantifying and normalizing at least one product of ethanol metabolism are provided. A method is provided for quantifying and normalizing at least one product of ethanol metabolism in a sample comprising creatinine. The method comprises adding a predetermined amount of at least one internal standard to the sample; adding deuterated creatinine to the sample; detecting and measuring at least one product of ethanol metabolism, the predetermined amount of at least one internal standard in the sample, the deuterated creatinine, and the creatinine. The method also comprises quantifying the amount of at least one product of ethanol metabolism in the sample using the measurement of the at least one internal standard; quantifying the amount of creatinine in the sample using the measurement of the deuterated creatinine; and normalizing the quantity of the at least one product of metabolism using the measurement of the creatinine. | 2009-04-02 |
| 20090087914 | AUTOMATIC ANALYZING APPARATUS AND QUALITY CONTROL METHOD FOR ANALYSIS SUPPORTING LIQUID IN THE SAME - An automatic analyzing apparatus can properly determine a quality of an analysis supporting liquid, after being supplied to the automatic analyzing apparatus, which supports an analysis performed in the automatic analyzing apparatus and can properly maintain an accuracy of the analysis. The analysis supporting liquid which supports an analysis of a specimen is dispensed into a reaction vessel by using a specimen dispensing unit which dispenses the specimen, a reagent dispensing unit which dispenses a reagent, a cleaning unit which cleans the reaction vessel, or a diluted solution dispensing unit which dispenses a diluted solution. An optical measurement is performed with respect to a liquid including the dispensed analysis supporting liquid in the reaction vessel. Analysis data of the analysis supporting liquid is generated by using the measured result. The quality of the analysis supporting liquid is determined by comparing the generated analysis data with reference data. | 2009-04-02 |
| 20090087915 | ANALYZER AND COMMUNICATION METHOD - An analyzer for analyzing a specimen includes a central control unit that instructs systems of the analyzer of a process operation; and a primary control unit that time-divisionally outputs an instruction by the central control unit. The analyzer also includes a plurality of secondary control units; a communication connection unit; and a plurality of connecting units. The secondary control units are connected to the systems, respectively, and control an operation of the systems according to the instruction by the central control unit. Each of the secondary control units has positional information set in advance. The communication connection unit connects the primary control unit and the secondary control units. The connecting units are provided on a fixed arrangement position, have arrangement positional information indicating the arrangement position, and are connected to the secondary control units, respectively. | 2009-04-02 |
| 20090087916 | Assay method for identifying drug candidate - The present invention provides a method of identifying a drug candidate capable of removing peptide, oligopeptide, polypeptide or protein from fibril or aggregate, which includes measuring, in the presence of a test compound, the concentration of a soluble peptide, a soluble oligopeptide, a soluble polypeptide or a soluble protein in an equilibrium state in a solvent. Moreover, the present invention provides a dissolution promoter to remove peptide, oligopeptide, polypeptide or protein from fibril or aggregate, which contains the compound obtained by the identification method as an active ingredient. | 2009-04-02 |
| 20090087917 | AUTOMATED PROTEIN ANALYZER - A protein analysis instrument and method are disclosed. The method includes mixing a binding dye composition and a protein sample using a homogenizer, measuring a parameter selected from the group consisting of the speed of the homogenizer and the resistance of the mixture to the homogenizer, adjusting the speed of the homogenizer based upon the measured parameter, pumping unreacted dye composition from the mixture and to a calorimeter, and measuring the absorbance of the dye composition in the calorimeter. Aspects of the invention also include inserting a spout into a sample cup at a position where the spout opening is positioned to avoid foam and precipitate generated by the mixing step and above the bottom of the sample cup and thereafter pumping the dye composition from the mixture in the sample cup through the spout and to a colorimeter. | 2009-04-02 |
| 20090087918 | DIFFERENTIATION OF ACUTE AND CHRONIC MYOCARDIAL NECROSIS IN SYMPTOMATIC PATIENTS - The present invention relates to a method for diagnosing an acute cardiovascular event comprising the steps of determining the amount of a cardiac troponin in a sample of a subject, determining the amount of a natriuretic peptide in a sample of said subject and diagnosing an acute cardiovascular event by comparing the amounts determined in the previous steps with reference amounts. Moreover, the present invention encompasses a method for differentiating between an acute cardiovascular event and chronic heart failure comprising the steps of determining the amount of a cardiac troponin in a sample of a subject, determining the amount of a natriuretic peptide in a sample of said subject and differentiating between an acute cardiovascular event and chronic heart failure by comparing the amounts determined in the previous steps with reference amounts. Also comprised by the present invention are devices and kits for carrying out such methods. | 2009-04-02 |
| 20090087919 | Method and Apparatus for Measuring Protein Post-Translational Modification - The present invention includes a method for analyzing reactions. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The solution further includes at least one controller chemical that affects the reaction between the donor chemical and the acceptor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The donor chemical is capable of donating a chemical moiety to the acceptor chemical. The donor chemical includes a functional group selected from ester, anhydride, imide, acyl halide, and amide. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. Yet another aspect of the present invention includes a method for analyzing protein function. The method includes the steps of providing a solution of at least one acceptor chemical and at least one donor chemical. The solution is then incubated so that a portion of the acceptor chemical reacts with the donor chemical to form an acceptor product. Unreacted donor chemical is separated from the acceptor product. The acceptor product or the donor chemical is then measured using X-ray fluorescence. An additional analytical method is also used to measure either the acceptor product or the donor chemical. | 2009-04-02 |
| 20090087920 | Method and apparatus for assessing blood-concentration of a volatile constituent - A method and apparatus for of assessing the blood concentration level in a human or animal subject, of a volatile blood constituent (preferably alcohol) is disclosed. The method comprises steps of positioning a sensor ( | 2009-04-02 |
| 20090087921 | VAPORIZED HYDROGEN PEROXIDE CONCENTRATION DETECTOR - A method of determining the presence of vaporized hydrogen peroxide (VHP) in a region, comprising the steps of providing a sealable region having an inlet port and an outlet port, creating a flow of a carrier gas into, through and out of the region, delivering vaporized hydrogen peroxide into the carrier gas flow upstream of the region inlet port, destroying the vaporized hydrogen peroxide at a first location downstream from the region outlet port, monitoring the temperature of the carrier gas before and after the first location, and determining a presence of vaporized hydrogen peroxide in the region based upon the temperature readings before and after the first location. | 2009-04-02 |
| 20090087922 | SINGLE NUCLEOTIDE POLYMORPHISM ANALYSIS OF HIGHLY POLYMORPHIC TARGET SEQUENCES - Methods and probes are provided for the analysis of target sequences having two or more polymorphisms wherein one of the polymorphisms is to be distinguished and another polymorphism is to be masked. | 2009-04-02 |
| 20090087923 | METHOD OF HIGH SENSITIVE IMMUNOASSAY - An object of the present invention is to provide a method of high sensitive immunoassay using immunoagglutination reaction by antigen-antibody reaction for quantification of thyroid stimulating hormone (TSH). The present invention provides a method of assaying a thyroid stimulating hormone (TSH) comprising assaying agglutination which is generated by contacting TSH with a carrier to which an anti-TSH antibody has been bound, wherein a plurality of types of anti-TSH antibodies that recognize different epitopes of TSH are independently supported on separate carriers, and each carrier on which a TSH antibody has been supported is brought into contact with a TSH-containing analyte with time intervals. | 2009-04-02 |
| 20090087924 | MICROFLUIDIC REVERSE AFFINITY-BLOT DEVICE - The microfluidic reverse affinity-blot device of the present disclosure combines affinity binding for isolation and/or enrichment of protein(s) from a sample, followed by separation/identification thereof. In general terms, a microfluidic reverse affinity-blot device is a closed system of interconnected components that is comprised of defined points of entry and exit, wherein the interconnected components include a capture region upstream from a protein separation region and subsequent detection region. Methods of use are also described. | 2009-04-02 |
| 20090087925 | DEVICES AND METHODS FOR ANALYSIS OF SAMPLES WITH DEPLETION OF ANALYTE CONTENT - A system and method for determining the presence and/or concentration of one or more analytes in a sample that comprises a fluid, the system comprising a solid substrate comprising a sample inlet or inlets and one or more analyte determination flow paths, each analyte determination flow path comprising a defined beginning and a defined terminus and comprising at least one capture zone containing a capture agent for an analyte, the capture agent or agents being immobilized along a portion of the flow path or paths, the flow path or paths being designed so that the one or more analytes are depleted from the sample and bound in a non-linear manner to the portion of the flow path or paths containing immobilized capture agent or agents, producing an analyte depletion end region for each analyte between the beginning and the terminus of the analyte determination flow path. | 2009-04-02 |
| 20090087926 | IMMUNOCHROMATOGRAPHIC TEST DEVICE - The invention provides an immunochromatographic test device for detecting a test substance in a sample, comprising a chromatographic membrane carrier; a sample developing member; and a labeling substance holding member; wherein a substance being capable of reacting to a human anti-mouse antibody is held in a member disposed at a position which is more upstream on the basis of the sample developing direction than the chromatographic membrane carrier, as well as an a method for detecting a test substance in a sample by using an immunochromatographic test device and a method for manufacturing an immunochromatographic test device. | 2009-04-02 |
| 20090087927 | IMMUNOCHROMATOGRAPHY METHOD USING FRAGMENTED ANTIBODY - An object of the present invention is to overcome the problem of nonspecific adsorption of a labeled antibody on the detection site and to provide an immunochromatography method by which the false positive signal can be suppressed, and wherein the detection sensitivity is high and the highly reliable measurement can be performed. The present invention provides an immunochromatography method, which comprises developing an analyte and a labeling substance modified with a first antibody against the analyte on a porous carrier in a state where the analyte and the labeling substance is mixed, and capturing the analyte and the labeling substance at the reaction site on the porous carrier having a second antibody against the analyte, so as to detect the analyte; wherein a fragmented antibody is used as the first antibody and/or the second antibody; an analyte is detected via amplification using an amplification solution which contains a compound containing silver and a reducing agent for silver ions; and the ratio of the non-specifically adsorbed labeling substance in the pre-amplification detection site and the non-detection site of the porous carrier is between 0.4 to 2.5 in a system where the density of the non-specifically adsorbed labeling substance is 10 | 2009-04-02 |
| 20090087928 | COPPER CONTAMINATION DETECTION METHOD AND SYSTEM FOR MONITORING COPPER CONTAMINATION - A method of monitoring copper contamination. The method includes method, comprising: (a) ion-implanting an N-type dopant into a region of single-crystal silicon substrate, the region abutting a top surface of the substrate; (c) activating the N-type dopant by annealing the substrate at a temperature of 500° C. or higher in an inert atmosphere; (c) submerging, for a present duration of time, the substrate into an aqueous solution, the aqueous solution to be monitored for copper contamination; and (d) determining an amount of copper adsorbed from the aqueous solution by the region of the substrate. | 2009-04-02 |
