| 13th week of 2009 patent applcation highlights part 41 |
| Patent application number | Title | Published |
|---|
| 20090081709 | Protein markers of responsiveness to type III receptor tyrosine kinase inhibitors - The invention discloses ten (10) protein markers predictive of cancer resistance or responsiveness to Type III Receptor Tyrosine Kinase (RTK) inhibitors, and provides methods for identifying a cancer that is likely to be resistant to a Type III RTK-inhibiting therapeutic by examining expression and/or activity of one or more of the disclosed biomarkers in a biological sample from the cancer. Methods for identifying a compound that inhibits a cancer resistant to a Type III RTK-inhibiting therapeutic by determining the effect of the compound on one or more of the disclosed marker proteins are also provided. | 2009-03-26 |
| 20090081710 | Method for Cancer Prognosis Using Cellular Folate Vitamin Receptor Quantification - The invention relates to a method for determining a prognosis for a cancer by quantifying vitamin receptor expression on the cancer cells. The method comprises the steps of quantifying vitamin receptor expression on the cancer cells, and determining a prognosis for the cancer. The invention also relates to methods and kits for determining the presence of vitamin receptors on cancer cells to select patients that should be treated with a therapy that utilizes vitamin receptor targeting and to develop a treatment regimen for such patients. The invention further relates to kits for performing the methods. | 2009-03-26 |
| 20090081711 | ADDRESSABLE ANTIBODY ARRAYS AND METHODS OF USE - Systems and assay methods are disclosed for detecting an autoantibody in a sample. In certain instances, the systems and methods employ a mass tag releasably connected to an antigen. The tag is thereafter released for detection. A tag can be detected by mass spectrometry or in certain instances the tag is fluorescent. Methods for diagnosing a disease or disorder in a subject are also disclosed. | 2009-03-26 |
| 20090081712 | ATM-Dependent Phosphorylation of Sp1 Is Involved in the Cellular Response to DNA Damage and Enhances Cellular Survival After DNA Damage - The present invention is related to the discovery that phosphorylation of SP1 (SEQ ID NO.: 2) at serine residue 101 (known herein as phosphoserine | 2009-03-26 |
| 20090081713 | PEPTIDE BIOMARKERS PREDICTIVE OF RENAL FUNCTION DECLINE AND KIDNEY DISEASE - The presently-disclosed subject matter provides methods for diagnosing a kidney disease, or the risk thereof, in a subject. The methods comprise determining an amount of at least one peptide biomarker disclosed herein in a biological sample from the subject and comparing the amount of the at least one peptide in the sample with a control level, wherein if the amount determined is different than the control level, the subject is diagnosed as having, or at an increased risk of developing, the kidney disease. | 2009-03-26 |
| 20090081714 | Assays - Methods and assays for monitoring the cardiac health of a subject are provided. The method involves the detection of a urotensin surrogate in a sample leading to a more accurate and reliable diagnosis of early or late stages of decompensated heart failure, heart failure, risk of a heart failur in a subject than the measurement of the cyclic peptide of urotensin alone. | 2009-03-26 |
| 20090081715 | Engineered Light-Emitting Reporter Genes - Compositions and methods are provided for enhanced expression of light emitting reporters. Such reporters are used in methods for monitoring cultures for production of target compounds. | 2009-03-26 |
| 20090081716 | Method for Monitoring the Immune Response and Predicting Clinical Outcomes in transplant recipients - Methods for monitoring the immune response and predicting clinical outcomes for patients on immunosuppressive drugs (such as transplant patients) are provided. The methods are based on the measurement of an intracellular metabolic marker in lymphocytes (such as ATP) as an indicator of a patient's immune response. | 2009-03-26 |
| 20090081717 | Compositions and Methods For Signal Generation - Improved assay systems comprise a substrate that is modified to include a solubilizing group and/or an at least binary solvent system to provide significantly enhanced signal strength and signal-to-noise ratio. In especially preferred aspects, the assay system is a chromogenic, electrochemical, and/or luminogenic assay system in which an indoxyl-type substrate has a solubilizing group covalently attached to the substrate and/or in which a polar and non-protic solvent is used as a co-solvent. | 2009-03-26 |
| 20090081718 | Protein cleavage method and thereof - The present invention provides a method for cleaving a glycated protein to obtain an amino acid or a peptide efficiently with a protease. By treating the glycated protein with the protease in the presence of a compound represented by R—X, the amino acid or the peptide is obtained by the cleavage. The R represents an alkyl compound with a carbon number of 9 or more, and preferably is straight-chain alkyl or straight-chain acyl with a carbon number of 9 to 16, branched-chain alkyl or branched-chain acyl with a carbon number of 10 to 40 and a main-chain carbon number of 9 to 16, or straight-chain alkyl that is substituted by cycloalkyl (a carbon number of the cycloalkyl ranges from 3 to 8, and a carbon number of the straight chain ranges from 4 to 13), where X is a sugar residue. Moreover, the glycated protein is, for example, glycated hemoglobin, and preferably β-chain N-terminal amino acid or a β-chain N-terminal peptide is cleaved by the protease treatment. | 2009-03-26 |
| 20090081719 | BIOCHEMICAL MARKERS FOR ACUTE PULMONARY EMBOLISM - The present invention relates to a method of differentiating between a singular and a multiple lung embolism in a subject suspected to suffer from acute lung embolism comprising determining the amount of NT-proBNP in a sample of a subject suspected to suffer from acute lung embolism and comparing the amount to a reference amount. Further, the present invention also relates to a method of differentiating between acute and chronic lung embolism in a subject comprising determining the amount of NT-proANP at a first and a second time point and comparing the determined amounts with each other. The present invention also encompasses devices and kits for carrying out the aforementioned methods. | 2009-03-26 |
| 20090081720 | Methods for identifying stem cells based on nuclear morphotypes - Methods for identifying stem cells and other cells specific to embryogenesis and carcinogenesis, classifying tissue samples, diagnosing precancerous and cancerous or atherosclerotic lesions, testing the value of anticancer agents, discovering macromolecules specifically expressed in particular cell types, using stem cells in restorative tissue therapy as well as methods for preparing tissue samples so heteromorphic nuclear morphotypes remain intact are disclosed. | 2009-03-26 |
| 20090081721 | HIGH-THROUGHPUT CELL ASSAYS - This invention provides high-through put methods and systems to identify and/or classify cells present in a sample. In one aspect, the method identifies the cell by determining the amount of thermal energy required to disrupt cell membranes. In another aspect, a method is for determining if an agent such as a drug will inhibit the growth of a cell by monitoring the amount of energy required to maintain a substantially constant temperature in a sample containing the cell grown in the presence of an agent or drug is provided. | 2009-03-26 |
| 20090081722 | SITE-SPECIFIC LABELING OF AFFINITY TAGS IN FUSION PROTEINS - The present invention provides methods and fluorescent compounds that facilitate detecting and labeling of a fusion protein by being capable of selectively binding to an affinity tag. The fluorescent compounds have the general formula A(B)n, wherein A is a fluorophore, B is a binding domain that is a charged chemical moiety, a protein or fragment thereof and n is an integer from 1-6 with the proviso that the protein or fragment thereof not be an antibody or generated from an antibody. The present invention provides specific fluorescent compounds and methods used to detect and label fusion proteins that contain a poly-histidine affinity tag. These compounds have the general formula A(L)m(B)n wherein A is a fluorophore, L is a linker, B is an acetic acid binding domain, m is an integer from 1 to 4 and n is an integer from 1 to 6. The acetic acid groups interact directly with the positively charged histidine residues of the affinity tag to effectively label and detect a fusion protein containing such an affinity tag when present in an acidic or neutral environment. | 2009-03-26 |
| 20090081723 | BIOREACTOR, CELL CULTURE METHOD, AND SUBSTANCE PRODUCTION METHOD - The present invention achieves a smaller difference between a dissolved oxygen concentration in an upper part of a culture tank and a dissolved oxygen concentration in a lower part of the culture tank. The present invention comprises: a culture tank; a sparger means arranged in a lower part of the culture tank; and multiple impellers being arranged in multiple stages in a vertical direction of the culture tank and having a larger mass transfer capacity coefficient K | 2009-03-26 |
| 20090081724 | Mutations in Ion Channels - A method of identifying a subject predisposed to a disorder associated with ion channel dysfunction, comprising ascertaining whether at least one of the genes encoding ion channel subunits in said subject has undergone a mutation event as set forth in one of SEQ ID Numbers: 1-72. | 2009-03-26 |
| 20090081725 | Compositions of pamps and Listeria monocytogenes and methods of use - A composition comprising a pathogen associated molecular pattern (PAMP) protein that activates toll-like receptor 2 (TLR2) or toll-like receptor 5 (TLR5) signaling and at least two distinct antigens of | 2009-03-26 |
| 20090081726 | Recombinant Microorganism - A host microorganism capable of increasing productivity of a protein or polypeptide, a recombinant microorganism obtained by introducing a gene encoding a protein or polypeptide into the host microorganism, and a method for producing a protein or polypeptide using the recombinant microorganism are provided. | 2009-03-26 |
| 20090081727 | CD33-Like Protein - The present invention concerns a novel CD33-like protein. In particular, isolated nucleic acid molecules are provided encoding the CD33-like protein. Recombinant CD33-like polypeptides are also provided as are recombinant vectors and host cells. The invention further provides methods useful during tumor or inflammatory disease diagnosis or prognosis and therapeutic treatments targeting cells expressing CD33-like polypeptides. | 2009-03-26 |
| 20090081728 | PRODUCTION OF ATTENUATED NEGATIVE STRANDED RNA VIRUS VACCINES FROM CLONED NUCLEOTIDE SEQUENCES - Attenuated, recombinant negative stranded RNA viruses suitable for vaccine use are produced from one or more isolated polynucleotide molecules encoding the virus. A recombinant genome or antigenome of the subject virus is modified to encode a mutation within a recombinant protein of the virus at one or more amino acid positions(s) corresponding to a site of an attenuating mutation in a heretologous, mutant negative stranded RNA virus. A similar attenuating mutation as identified in the heterologous negative stranded RNA virus is thus incorporated at a corresponding site within the recombinant virus to confer an attenuated phenotype on the recombinant virus. The attenuating mutation incorporated in the recombinant virus may be identical or conservative in relation to the attenuating mutation identified in the heterologous, mutant virus. By the transfer of mutations into recombinant negative stranded RNA viruses in this matter, candidate vaccine viruses are engineered to elicit a desired immune response against a subject virus in a host susceptible to infection thereby. | 2009-03-26 |
| 20090081729 | Compositions and Methods Comprising a Ligand of ChemerinR - The present invention relates to a G-protein coupled receptor and a novel ligand therefor. The invention provides screening assays for the identification of candidate compounds which modulate the activity of the G-protein coupled receptor, as well as assays useful for the diagnosis and treatment of a disease or disorder related to the dysregulation of G-protein coupled receptor signaling. | 2009-03-26 |
| 20090081730 | ACTIVATABLE RECOMBINANT NEUROTOXINS - Compositions comprising activatable recombinant neurotoxins and polypeptides derived therefrom. The invention also comprises nucleic acids encoding such polypeptides, and methods of making such polypeptides and nucleic acids. | 2009-03-26 |
| 20090081731 | Method of producing catalytically active BACE2 enzymes - The invention provides methods and compositions for the efficient expression and isolation of BACE2 polypeptides from inclusion bodies and its refolding to an active enzyme. | 2009-03-26 |
| 20090081732 | ANTIBODIES TO HUMAN IL-1BETA - An IL-1β binding molecule, in particular an antibody to human IL-1β, especially a human antibody to human IL-1β is provided, wherein the CDRs of the heavy and light chains have amino acid sequences as defined, for use in the treatment of an IL-1 mediated disease or disorder, e.g. osteoarthritis, osteoporosis and other inflammatory arthritides. | 2009-03-26 |
| 20090081733 | METHODS FOR PRODUCING AND USING POLYMYXINS - The present invention provides methods for producing a polymyxin from an isolated | 2009-03-26 |
| 20090081734 | Arabinitol Dehydrogenases from Neurospora crassa - Stable and active arabinitol dehydrogenases (LAD) from | 2009-03-26 |
| 20090081735 | NOVEL PROCESS - Novel organisms, including DNA construct host cell combinations, are disclosed. The organisms comprise a transcription unit (e.g. operon) comprising DNA sequences encoding for enzymes which promote the supply of single carbon units for the conversion of dUMP to dTMP. Examples include: dihydrofolate reductase genes e.g. T4 frd; Serine Hydroxymethyltransferase genes e.g. glyA; 3-phosphoglycerate dehydrogenase genes e.g. serA; and THF synthase genes e.g. ADE3. The organisms are used in a biological method of producing thymidine with significantly reduced levels of uridine. | 2009-03-26 |
| 20090081736 | Methods For Using Mutant RNA Polymerases Wtih Reduced Discrimination Between Non-Canonical Nucleoside Triphosphates - A method for synthesizing a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate using a mutant polymerase having a reduced discrimination between canonical and non-canonical substrates is disclosed. The method comprises incubating a template nucleic acid in a reaction mixture comprising the mutant nucleic acid polymerase and the appropriate canonical and non-canonical nucleoside triphosphates which are desired substrates for the mutant nucleic acid polymerase. The present invention is also a method of determining the sequence of a nucleic acid molecule using the mutant polymerase to create a nucleic acid molecule comprising at least one non-canonical nucleoside triphosphate. | 2009-03-26 |
| 20090081737 | METHODS AND COMPOSITIONS FOR REDUCING THE COMPLEXITY OF A NUCLEIC ACID SAMPLE - Aspects of the present invention are drawn to methods and compositions for sorting nucleic acid molecules into physically separate compartments according to the identity of a nucleotide base or sequence of bases at a specific location, resulting in the production of reduced complexity samples that find use in any number of downstream genetic analyses. Aspects of the methods of the invention include fragmenting a nucleic acid sample, e.g., with a restriction enzyme, ligating an adaptor (or adaptors), and sorting the fragments based on the identity of the nucleotide base(s) positioned adjacent to the fragmentation site (e.g., the restriction enzyme cut site/or recognition site). Each round of sorting produces binned samples having reduced complexity over the parent sample. | 2009-03-26 |
| 20090081738 | Method for Producing an L-Amino Acid Using a Bacterium of the Enterobacteriaceae Family Having Expression of the bolA Gene Attenuated - The present invention provides a method for producing an L-amino acid using a bacterium of the Enterobacteriaceae family, particularly a bacterium belonging to the genus | 2009-03-26 |
| 20090081739 | PROCESS FOR PRODUCING AMINO ACIDS - The present invention provides: a process for producing an amino acid which comprises adding crystals of the amino acid having an average particle size of 1 to 120 μm to a medium so that the concentration of the crystals of the amino acid becomes 0.5 g/l or more, culturing a microorganism having the ability to produce the amino acid in the medium, allowing crystals of the amino acid to form and accumulate in the medium, and recovering the crystals of the amino acid from the culture; and a process for producing an amino acid which comprises adding crystals of the amino acid to a medium so that the total surface area of the crystals of the amino acid in the medium becomes 0.02 m | 2009-03-26 |
| 20090081740 | PRODUCTION OF AMINO ACIDS FROM SUCROSE IN CORYNEBACTERIUM GLUTAMICUM - Methods and compositions for increased production of amino acids from | 2009-03-26 |
| 20090081741 | SINGLE COLONIES OF MYXOBACTERIA CELLS - A single colony of myxobacterium cells, a process for its production and its use. A process for the production of a myxobacterium | 2009-03-26 |
| 20090081742 | HIGH EFFICIENCY SEPARATIONS TO RECOVER OIL FROM MICROALGAE - A system and method for processing algae cells to create biofuel are disclosed. Specifically, the system and method utilize steam to rupture algae cells in order to utilize intracellular oil therein. The system includes a conduit for growing algae cells and a generator for creating steam. Further, the system includes a lysing device that mixes the algae cells and the steam to rupture the algae cells. In order to maximize the efficiency of the lysing process, the system may further include a heat exchanger for preheating the algae cells with the lysed cells. In addition, the system includes a bioreactor to synthesize biofuel from the unbound oil. | 2009-03-26 |
| 20090081743 | TRANSPORTABLE ALGAE BIODIESEL SYSTEM - A portable system and method for producing biofuel from algae are disclosed. In the portable system, a chemostat and a plug flow reactor formed from plastic bladders are interconnected. Further, an algae separator is in fluid communication with the plug flow reactor for removing algae cells. Also, the system includes a device for processing biofuel from the algae cells. Importantly, the system includes a temperature controller to maintain desired temperatures in the chemostat and plug flow reactor for algae growth and intracellular algae production. In order to further support algae cell growth, the system includes a device for capturing carbon dioxide and delivering the carbon dioxide to the chemostat. | 2009-03-26 |
| 20090081744 | Method of processing slops - A method of processing slops including production of biogas characterized in that slops are exposed to anaerobic fermentation in a anaerobic fermenter under generation of biogas and at least a portion of the biogas containing methane and carbon dioxide is fed into a first photo-bioreactor, where the carbon dioxide or a part thereof is consumed during a photosynthesis process producing micro-algae and methane and the residual carbon dioxide are hen withdrawn for further processing. | 2009-03-26 |
| 20090081745 | METHOD FOR PRODUCING 1,3-PROPANEDIOL AND 2,3-BUTANEDIOL FROM RAW STARCH MATERIAL - The invention discloses a method for producing 1,3-propanediol and 2,3-butanediol from raw starch materials, including the following steps: 1) Candida krusei or Hansenula Arabitolgens Fang are inoculated into a fermentation medium with the saccharifying liquid of the raw starches as a carbon source; the yeast cells are cultured on an aerobic condition until glucose-consuming-rate is significantly reduced, and then fermented anaerobically to a glucose concentration from 5 to 10 g/L; the fermentation broth is collected and filtered to remove the yeast cells in the broth, and the resultant filtrate is glycerin fermentation broth; 2) Klebsiella, Clostridium butyricum, or Clostridium pasteurianum are inoculated into a fermentation medium in which the glycerin fermentation broth obtained from step 1) serves as a carbon source; the bacteria are fermented anaerobically for 30-32 hours, and then fermented aerobically when the production rate of 1,3-propanediol decreased obviously, and the fermentation was stopped when the concentration of glycerin is reduced to a level below 10 g/L, and finally 1,3-propanediol and 2,3-butanediol are obtained. The method of the present invention can effectively reduce production cost and increase productivity. | 2009-03-26 |
| 20090081746 | BIOFUEL PRODUCTION BY RECOMBINANT MICROORGANISMS - Provided herein are metabolically-modified microorganisms useful for producing biofuels. More specifically, provided herein are methods of producing high alcohols including isobutanol, 1-butanol, 1-propanol, 2-methyl-1-butanol, 3-methyl-1-butanol and 2-phenylethanol from a suitable substrate. | 2009-03-26 |
| 20090081747 | ETHANOL FROM CORN AND USE OF DISTILLER GRAINS BY-PRODUCT TO PRODUCE FUEL GAS - A process for substantially increasing the production of ethanol from corn and other such biomass feedstocks. Ethanol, carbon dioxide and distiller grains are typically produced during the fermentation process. The carbon dioxide is used as a feed for additional ethanol make and the distiller grains can be used as a livestock feed or to produce a synthetic fuel gas that is used to run a boiler to produce steam used in the fermentation process. | 2009-03-26 |
| 20090081748 | INTEGRATED PROCESSES AND SYSTEMS FOR PRODUCTION OF BIOFUELS USING ALGAE - A process for production of biofuels from biomass can include depolymerizing a biomass to form a feed. The feed can be formed by autotrophically growing algal biomass and extracting the feed therefrom. The algal feed can be converted to an algal oil by introducing the algal feed to an oil-producing algae under growth conditions sufficient to encourage formation of algal oil within the oil-producing algae. The algal oil can be extracted from the oil-producing algae and can further be converted to biodiesel. | 2009-03-26 |
| 20090081749 | PROCESS FOR PRODUCING ETHANOL FROM CORN DRY MILLING - A process for producing ethanol by the conversion of carbohydrates from a corn dry milling process in which the bottoms fraction from distillation of ethanol in a conventional yeast fermentation is used in a process including a combination of biochemical and synthetic conversions. The process results in high yield ethanol production with concurrent production of high value coproducts. An acetic acid intermediate is produced from bottoms fraction, followed by conversion of the acetic acid into ethanol using esterification and hydrogenation reactions. Coproducts of the process include a high protein content solids fraction produced in the fermentation. | 2009-03-26 |
| 20090081750 | TRANSFECTION IN MAGNETICALLY DRIVEN CONTINUOUS FLOW - Biological cells and other membranous structures are transfected in a flow-through system by first rendering the structures magnetically active such that they respond to a magnetic field, suspending the structures in a solution of an exogenous species with which the structures are to be transfected, then placing the suspension in a channel and using a moving magnetization pattern along the channel wall to cause the structures to travel through the channel. Along their path of travel, the structures pass a transmitter that emits transfection energy sufficient to cause the exogenous species in the suspension to permeate the structure membranes and enter the interiors of the structures. | 2009-03-26 |
| 20090081751 | NATIVELY GLYCOSYLATED MAMMALIAN BIOLOGICAL MOLECULES PRODUCED BY ELECTROMAGNETICALLY STIMULATING LIVING MAMMALIAN CELLS - A composition is disclosed with the composition comprising a mixture of natively glycosylated mammalian biological molecules produced by electromagnetically stimulating living mammalian cells. | 2009-03-26 |
| 20090081752 | BIOREACTOR, KIT AND METHOD OF USING SAME - A bioreactor, kit, a method of using the device to promoting growing and/or culture of a cell, and a method for regenerating and/or improving the function of mammalian cells. The bioreactor includes a controlling circuit coupled to magnetic field emitter that emits relatively steep and short-lived magnetic field pulses during these an active ephemeral period. The bioreactor also provides a relatively long-term inactive phase in which no magnetic field pulses are imposed. The kit includes the unassembled components of the bioreactor. The method of using the bioreactor and of regenerating and/or improving the function of mammalian cells includes the step of applying a time variant magnetic field through the cell to promote growing and/or culturing, and then introducing the cells to a mammal to regenerate cells. | 2009-03-26 |
| 20090081753 | METHOD FOR FORMULATING AND IMMOBILIZING A MATRIX PROTEIN AND A MATRIX PROTEIN FOR USE IN A SENSOR - A method for formulating and immobilizing a protein and a protein matrix formed by the method. The protein matrix preparation method results in a physically and chemically stable protein matrix that has low swelling, non-leaching, high activity, and high mechanical strength properties. The method includes cross-linking and hardening the protein mixture and using a mold to form a protein into a desired shape and size. | 2009-03-26 |
| 20090081754 | GENE OF ENZYME HAVING ACTIVITY TO GENERATE LACHRYMATORY FACTOR - It is an object of the present invention to provide isozymes of the lachrymatory factor producing enzyme, the amino acid sequences of these isozymes and a gene that codes for these amino acid sequences, and the present invention relates to three types of isozymes of the lachrymatory factor producing enzyme that contributes to the production of the lachrymatory factor that is present in onions and the like, amino acid sequences indicated by SEQ ID Nos. 1 to 3 which constitute the proteins or polypeptides of these isozymes, DNA indicated by SEQ ID Nos. 4 and 5 which contains base sequences that code for the abovementioned proteins or polypeptides, a method of producing the abovementioned isozymes, a recombinant vector which contains the abovementioned DNA, a transformant formed by transforming a host cell with the abovementioned recombinant vector, a method of producing proteins or polypeptides that have lachrymatory factor producing enzyme activity by culturing the abovementioned host cell, and anti-sense RNA which has a base sequence that is complementary to that of the mRNA corresponding to the abovementioned DNA. | 2009-03-26 |
| 20090081755 | FRAGRANT CONSUMER PRODUCTS COMPRISING OXIDIZING AGENTS - Fragrant consumer products comprising oxidizing agents are described, which may be, for example, detergents or cleaners or, for example, also cosmetics. These consumer products comprise certain minimum amounts of fragrances of certain classes of substances. They are characterized by very good (storage) stability, both with regard to the fragrance of the product, and also with regard to the potency of the oxidizing agent. | 2009-03-26 |
| 20090081756 | Nucleic acid-enzyme complex - The present invention provides a method for controlling cleavage of a target RNA by deoxyribozyme. | 2009-03-26 |
| 20090081757 | Adsorbents Comprising Anthraquinone Dye-Ligends for the Separation of Biological Materials - A process for the separation of biological materials, such as dye-ligand affinity chromatography, wherein an adsorbent is used, which comprises a reaction product of certain reactive anthroquinone compounds and a substrate having a group capable of reaction with a reactive group in said reactive anthroquinone compounds to form a covalent bond. | 2009-03-26 |
| 20090081758 | Chimeric Cytochrome P450 Proteins and Methods of Use - The present invention provides chimeric cytochrome P450 enzymes fused to heterologous reductase domains to generate single-component, self-sufficient, more cost-effective and catalytically more active biosynthetic P450 monooxygenases. | 2009-03-26 |
| 20090081759 | NUCLEIC ACID ENCODING A NOVEL RIBONUCLEASE HAVING AN AMINO ACID SEQUENCE MADE UP OF THE AMINO ACID SEQUENCE OF A KNOWN RIBONUCLEASE AND AN N-TERMINAL LEADER SEQUENCE THAT IS AT LEAST ONE RESIDUE LONG - A nucleic acid encodes a novel RNase. The RNase has an amino acid sequence in which an amino acid sequence disclosed in United States Patent U.S. Pat. No. 6,239,257 B1 is preceded by a different N-terminal residue or leader sequence. | 2009-03-26 |
| 20090081760 | DMO METHODS AND COMPOSITIONS - The invention provides for identification and use of crystal structures of Dicamba monooxygenase (DMO) that may be complexed with iron or cobalt cofactor and substrate (dicamba), or product (DCSA) in order to define residues important for enzymatic structure and function. Methods of using such structures are described. Data storage media comprising the crystal structural coordinate information are also described. | 2009-03-26 |
| 20090081761 | Modified hydrogenase, enzymatic electrode made of modified hydrogenase, and hydrogenase modification method - The invention provides: (1) a modified hydrogenase obtained by removing electron-transfer sites from a hydrogenase constituted of: active subunits including active sites having a hydrogen oxidization-reduction activity; and electron-transfer subunits having the electron-transfer sites through which electrons are transferred between the active sites and the outside of the hydrogenase; (2) a modified hydrogenase obtained from a hydrogenase, wherein when the hydrogenase is isolated from bacteria that produces the hydrogenase, a process for exposing the hydrogenase to an oxygen atmosphere is executed; (3) an enzymatic electrode made of at least one of the foregoing modified hydrogenases; and (4) a hydrogenase modification method including: a step of isolating from hydrogenase-producing bacteria; and a step of removing the electron-transfer sites of the electron-transfer subunits from the hydrogenase by exposing the hydrogenase to an oxygen atmosphere. | 2009-03-26 |
| 20090081762 | Superactive Cellulase Formulation Using Cellobiohydrolase-1 from Penicillium Funiculosum - Purified cellobiohydrolase I (glycosyl hydrolase family 7 (Cel7A)) enzymes from | 2009-03-26 |
| 20090081763 | Novel protein-deamidating enzyme, microorganism producing the same, gene encoding the same, production process therefor, and use thereof - A method for the production of an enzyme, which comprises culturing in a medium a strain that belongs to a bacterium classified into Cytophagales or Actinomycetes, or a new bacterium | 2009-03-26 |
| 20090081764 | Heterologous G protein coupled receptors expressed in yeast, their fusion with G proteins and use thereof in bioassay - The present invention is directed to expression vectors and yeast cells transformed therewith containing a first heterologous nucleotide sequence which codes for a G protein-coupled receptor, for example, the somatostatin receptor, and a second nucleotide sequence which codes for all or a portion of a G protein αβγ complex. Said heterologous protein is physically expressed in a host cell membrane in proper orientation for both stereoselective binding of ligands, as well as functional interaction with G proteins on the cytoplasmic side of the cell membrane. In some embodiments, a nucleotide sequence encoding a heterologous or chimeric Gα protein is expressed in conjunction with nucleotide sequences from the yeast G protein βγ subunits. A second aspect of the present invention provides expression vectors and yeast cells transformed therewith encoding chimeric yeast/heterologous G protein coupled receptors. A third aspect of the present invention is directed to methods of assaying compounds using such expression constructs and yeast cell expression systems to determine the effects of ligand binding to the heterologous receptors expressed in the systems. | 2009-03-26 |
| 20090081765 | CELL ELECTROPHYSIOLOGICAL SENSOR AND METHOD FOR MANUFACTURING THE SAME - In a cell electrophysiological sensor having a thin plate with a through hole, a support plate with a through hole and a container plate with a through hole stuck to an upper portion of this support plate, the support plate and the container plate are stuck to each other through fusion with a portion of the outer shape of a first electrode in a ring shape intervening in a portion of the interface. In this configuration, a cell electrophysiological sensor which allows for measurement with high precision can be attained, and a manufacturing method which is excellent in terms of mass production can be provided. | 2009-03-26 |
| 20090081766 | MICRO BIO SENSOR AND METHOD FOR MANUFACTURING THE MICRO BIO SENSOR - A micro bio sensor which detects a microbe existing in a specimen is provided. The microbial sensor includes a base, a detector formed on the base, and a reaction layer formed on the detector, wherein the reaction layer is comprised of a self-assembled monolayer which is formed on the detector and an antibiotic which is immobilized through the self-assembled monolayer on the detector. By using the micro bio sensor, it is possible to detect species of the microbe concurrently and improve sensitivity for detecting the species of the microbe. Further, a method for manufacturing such a micro bio sensor is also provided. | 2009-03-26 |
| 20090081767 | ENDOSCOPE STERILIZATION EVALUATION DEVICE - An endoscope sterilization evaluation device is provided with an elongated hollow body having a hollow portion opened at opposite ends, the elongated hollow body simulating an endoscope having a tubular passage, an exterior member having a first housing portion in which a biological indicator for performing sterilization evaluation on the hollow body is housed, and a second housing portion in which a culture solution for cultivating germs attached to the biological indicator is housed, the exterior member also having at a predetermined position a communication passage for communication between the first housing portion and the outside, and a mount device for holding the communication passage in a state of communicating with the hollow portion, and mounting the exterior member to the hollow body. | 2009-03-26 |
| 20090081768 | Devices and Methods for Thermally Isolating Chambers of an Assay Card - An assay card and devices and methods for isolating chambers on the assay card are described. The assay card comprises a substrate formed of one or more materials, e.g., plastic, having a softening temperature, the substrate defining channels communicating with respective reaction chambers. The assay card may be heated in a region of the channels to at least the softening temperature. The softened plastic may be deformed, e.g., with a tool which may or may not also provide the heat for softening the substrate. In this manner, the plastic of the substrate may be caused to at least partially obstruct the channels, thereby isolating the reaction chambers. The invention also relates to a method of manufacturing a tool device that includes pins for heating and deforming an assay card. | 2009-03-26 |
| 20090081769 | Apparatus for judging cell detachment, method of judging cell detachment, and cell culture apparatus - An apparatus for judging cell detachment that judges a state of detachment of cells that have been cultured within a cell culture container (cultured cells), includes: an image-capturing unit that captures an image of the cultured cells; and a detachment state judging unit that determines luminance information within the cell culture container based upon image capture data from the image-capturing unit, and judges that the culture cells are detached when the luminance information exceeds a predetermined luminance level. | 2009-03-26 |
| 20090081770 | HIGH THROUGHPUT BIOREACTOR - A continuous flow bioreactor system that includes a bioreactor, an optional post-bioreactor preparation chamber, a cell sorter, and an optional pre-bioreactor preparation chamber in a closed loop, useful for enriching a heterogeneous cell population growing in the bioreactor with an isolated subpopulation of cells. | 2009-03-26 |
| 20090081771 | SYSTEM AND METHOD FOR HEATING, COOLING AND HEAT CYCLING ON MICROFLUIDIC DEVICE - An integrated heat exchange system on a microfluidic card. According to one aspect of the invention, the portable microfluidic card has a heating, cooling and heat cycling system on-board such that the card can be used portably. The microfluidic card includes one or more reservoirs containing exothermic or endothermic material. Once the chemical process of the reservoir material is activated, the reservoir provides heat or cooling to specific locations of the microfluidic card. Multiple reservoirs may be included on a single card to provide varying temperatures. The assay chemicals can be moved to the various reservoirs to create a thermal cycle useful in many biological reactions, for example, Polymerase Chain Reaction (PCR) or rtPCR. According to another aspect of the invention, the integrated heat exchanger is an adjacent microfluidic circuit containing fluid that is either independently heated or cooled, or is an exothermic or endothermic material, such that the fluid in the adjacent circuit imparts a change in temperature to the assay fluid in an independent circuit. According to yet another aspect of the invention, a thermal electric cooler (TEC) is used for thermocycling the amplification chamber of a disposable microfluidic card. | 2009-03-26 |
| 20090081772 | Method and device for collecting cellular material from cells isolated on a filter - Method for collecting cellular material of particular cells present in a liquid includes: | 2009-03-26 |
| 20090081773 | MICROFLUIDIC APPARATUS FOR MANIPULATING IMAGING AND ANALYZING CELLS OF A CYTOLOGICAL SPECIMEN - A microfluidic apparatus for isolating and imaging or analyzing cells of a cytological specimen includes a substrate and a microfluidic cellular isolation element that includes an outer wall, a channel, a partition member and a receptacle. The partition member is positioned within the isolation element interior, and the receptacle is positioned within the partition member interior. The isolation element is configured such that fluid introduced through the outer wall inlet flows through the channel in a first direction, and the partition member is situated such that fluid flows from the channel into the partition member interior through the partition member inlet aperture in a second direction different than the first direction. The receptacle positioned relative to the partition member inlet to catch and retain a cell carried by the fluid. | 2009-03-26 |
| 20090081774 | Automated Staining and Decolorization of Biological Material - An improved method and apparatus for staining samples of biological material for accurate analysis of the sample. Biological material is applied to a substrate, such as a microscope slide. The biological specimen is then stained with a selected staining composition, which may be gentian violet for a Gram's Stain analysis. The stained biological material is at least partially decolorized and the level of decolorization is analyzed optically. If necessary, the decolorizing step and the optical analysis steps are repeated until a selected level of decolorization is obtained. | 2009-03-26 |
| 20090081775 | Microscope system and screening method for drugs, physical therapies and biohazards - Method and device for automated cell analysis and determination of transport and communication between living cells by analyzing the formation of tunneling nanotubes (TNTs) between cells. This method comprising the steps of singularizing cells in a culture medium and staining the cells with a fluorescent or luminescent dyes for staining of cytoplasm and membranes as well as TNTs, flagella and other cell particles for 3-D cell microscopy. The method comprises further an image analysis system. | 2009-03-26 |
| 20090081776 | NUCLEIC ACID ENCODING A NOVEL RIBONUCLEASE HAVING AN AMINO ACID SEQUENCE MADE UP OF THE AMINO ACID SEQUENCE OF A KNOWN RIBONUCLEASE AND AN N-TERMINAL LEADER SEQUENCE THAT IS AT LEAST ONE RESIDUE LONG - A nucleic acid encodes a novel RNase. The RNase has an amino acid sequence in which an amino acid sequence disclosed in U.S. Pat. No. 6,239,257 B1 is preceded by a different N-terminal residue or leader sequence. | 2009-03-26 |
| 20090081777 | NUCLEIC ACID ENCODING CYSTEINIZED RIBONUCLEASE TO WHICH A TARGETING MOIETY CAN BE CONJUGATED - A nucleic acid encodes a cysteinized RNase to which a targeting moiety can be conjugated. | 2009-03-26 |
| 20090081778 | NUCLEIC ACID ENCODING A FUSION PROTEIN INCORPORATING A CYSTEINIZED RIBONUCLEASE - A nucleic acid encodes a novel fusion protein. The fusion protein contains a cysteinized variant of an RNase disclosed in U.S. Pat. No. 6,239,257 B1. | 2009-03-26 |
| 20090081779 | TUMOR SUPPRESSOR GENE, P28ING5 - This disclosure provides a novel tumor suppressor, referred to as p28ING5, nucleic acid molecules encoding this protein, and methods of making and using these molecules. Also provided are methods of ameliorating, treating, detecting, prognosing, and diagnosing diseases and conditions associated with abnormal p28ING5 expression, such as neoplasia. Kits are also provided. | 2009-03-26 |
| 20090081780 | CANINE RESPIRATORY CORONAVIRUS (CRCV) SPIKE PROTEIN, POLYMERASE AND HEMAGGLUTININ/ESTERASE - A canine respiratory coronavirus (CRCV) that is present in the respiratory tract of dogs with canine infectious respiratory disease and which has a low level of homology to the enteric canine coronavirus, but which has a high level of homology to all bovine coronavirus strains (e.g., Quebec and LY138) and human coronavirus strain OC43. | 2009-03-26 |
| 20090081781 | Synthetic and standardized prion infectuous material, and uses thereof as an injecting inoculum - The invention concerns a novel, synthetic, standardised, soluble, reproducible and easy-to-handle infectious material of the prion type, consisting of a cell lysate or culture supernatant from stable transgenic cells expressing a prion protein PrP and supporting replication of the pathogenic form, PrPsc, of the said Prp. | 2009-03-26 |
| 20090081782 | Methods for vitrification of human oocytes - Provided are methods for the vitrification of human oocytes, which comprises: (a) placing human oocytes on a transfer instrument; and (b) placing the transfer instrument and the human oocytes directly into a slushed nitrogen (N | 2009-03-26 |
| 20090081783 | Method for producing langerhans cells or interstitial dendritic cells or both from CD14+ monocytes - The present invention relates to a method for preparing Langerhans cells or interstitial dendritic cells, or both, from CD14 | 2009-03-26 |
| 20090081784 | GENERATION OF CLONAL MESENCHYMAL PROGENITORS AND MESENCHYMAL STEM CELL LINES UNDER SERUM-FREE CONDITIONS - Methods for obtaining multipotent mesenchymal stem cells under serum-free conditions and methods for identifying multipotent mesenchymal progenitor cells are disclosed. | 2009-03-26 |
| 20090081785 | Desiccated Biologics And Methods Of Preparing The Same - The present invention provides compositions comprising desiccated biologics comprising a cell, protein, virus, nucleic acid, carbohydrate, or lipid, or any combination thereof, along with at least one membrane penetrable sugar, and at least one membrane impenetrable sugar, wherein the moisture content is from 5% to 95%, and to methods of preparing the same, and to methods of treating animals using the same. | 2009-03-26 |
| 20090081786 | Peptide sequences for modulation of protein kinase C - Peptides found within an annexin protein can be used to modulate the activity of a protein kinase C are described. More particularly, peptides within annexin I, V, and VI, as well as variants and conservatively modified variants thereof, are described, and use of these peptides to regulate cellular responses mediated by β-protein kinase C, δ-protein kinase C, and α-protein kinase C, respectively, are described. | 2009-03-26 |
| 20090081787 | AGENT FOR PROMOTING HEPATIC CELL REPLICATION AND AGENT FOR IMPROVING INSULIN RESISTANCE - The present invention aims at providing a medicament and a method for promoting replication of hepatocytes and a medicament and a method for ameliorating insulin resistance. An effective amount of a neutralizing agent for CXCL10 belonging to a subfamily of chemokines which are heparin-binding proteins is administered to the hepatocytes to promote the replication of the hepatocytes. As the neutralizing agent for CXCL10, a factor which is specifically bound to CXCL10 and inhibits an activity of CXCL10 or a factor which inhibits CXCL10 expression is suitably used. By administering the neutralizing agent to impaired hepatic tissue, it is possible to restore and regenerate the hepatic tissue. Meanwhile, by administering an effective amount of the neutralizing agent for CXCL10 to the hepatocytes, the insulin resistance in type II diabetes and metabolic syndrome is ameliorated. | 2009-03-26 |
| 20090081788 | Nucleotide sequence encoding the enzyme I-SceI and the uses thereof - An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes. | 2009-03-26 |
| 20090081789 | Activation of nuclear factor kappa B - The present invention describes a method for targeting a tumor cell comprising contacting the tumor cell with a composition comprising a macrophage and a factor that upregulates nuclear factor-kappa B (NFκB) activity. | 2009-03-26 |
| 20090081790 | Polynucleotide for Target Gene - The present invention provides a single strand polynucleotide sequence comprising a target gene, a complementary strand nucleic acid sequence, and a component sequence. | 2009-03-26 |
| 20090081791 | INTRACELLULAR DELIVERY OF SMALL MOLECULES, PROTEINS, AND NUCLEIC ACIDS - An amino acid sequence Arg-Lys-Met-Leu-Lys-Ser-Thr-Arg-Arg-Gln-Arg-Arg (SEQ ID NO:1) functions as a protein transduction domain (PTD) and is capable of delivering small molecules, proteins, and nucleic acids to an intracellular compartment of a cell. An amino terminal lysine linker improves the efficiency of the PTD. A nuclear localization signal can be used to target the PTD to a cell's nucleus. The PTD can be used in PTD-cargo moiety complexes that can reversibly immortalize cells and increase cell viability in culture. | 2009-03-26 |
| 20090081792 | IMMUNE POTENTIATING COMPOSITIONS OF CANCER CELLS - A composition of matter is disclosed, comprising an immunostimulatory molecule and animal cells cultured in the presence of at least one interferon (IFN) for a time and under conditions sufficient to enhance the antigen presenting function of said cells. Also disclosed are immunopotentiating compositions and their use for treatment and/or prophylaxis of a disease or condition. | 2009-03-26 |
| 20090081793 | Method for improving acid and low pH tolerance in yeast - A method for increasing tolerance in yeast to organic acids and low pH comprising functionally transforming a yeast with at least one copy of a nucleotide sequence encoding a plasma membrane H | 2009-03-26 |
| 20090081794 | Sample analyzer and method for analyzing samples - An sample analyzer, which enables to confirm an analysis remaining time for each sample and a total analysis remaining time for all samples set in the analyzer, so that time management for both each sample and all samples can be easily performed, is disclosed. Specifically, an analyzing unit analyzes a sample by executing an analysis sequence including a predetermined number of analyzing steps, and an control device calculates an analysis remaining time for each sample based on the number of analyzing steps. Control device acquires a total analysis remaining time by calculating the analysis remaining time for the sample in which sample information is lastly inputted. The display member displays the analysis remaining time and the total analysis remaining time. | 2009-03-26 |
| 20090081795 | Identification of Humans Through Characteristic Compounds Detected in Human Scent - The present invention is directed to methods of identifying and/or comparing humans. More particularly, the present invention is directed to methods of collecting human scent compounds from a subject, extracting the compounds, analyzing the compounds, and correlating the compounds to a unique compound profile for the subject. These unique compound profiles can be used to distinguish one subject from another, or to identify a specific subject based upon a sample. | 2009-03-26 |
| 20090081796 | METHOD FOR THE DETERMINATION OF PHOSPHOLIPIDS IN AMNIOTIC FLUID SAMPLES - The present invention refers to a fast method for the determination of phospholipids in amniotic fluid samples by HPTLC (high performance thin layer chromatography). This innovative method presents high sensitivity, reproducibility and resolution. It has also the advantage of being of easy laboratorial interpretation, allowing the elimination of false results due to the contamination of the sample with blood or meconium. | 2009-03-26 |
| 20090081797 | REACTIVE SURFACE ON A POLYMERIC SUBSTRATE - Plasma treated cyclic polyolefin copolymer surfaces having enhanced binding density for binding biologically active agents and cells are provided. These plasma treated cyclic polyolefin copolymer surfaces may be further enhanced for binding biologically active agents or cells by the application of conjugates. Methods of making and characterizing treated polymer surfaces are also provided. | 2009-03-26 |
| 20090081798 | Protein S Functional Assay - The invention relates generally to a new functional protein S assay and kit that is based on the ability of endogenous protein S to prolong clotting time. In the assay procedure, a test plasma sample is diluted with protein S deficient plasma, followed by the addition of purified or recombinant tissue factor (pTF or rTF), purified natural or synthetic phospholipid (pPL or sPL) and activated protein C (APC) or protein C activator (PCA). The clotting time is then measured and compared to a standard curve or a normal control. | 2009-03-26 |
| 20090081799 | ANALYTE EVALUATION DEVICE AND ANALYTE EVALUATION METHOD - An analyte evaluation device includes: a light irradiator for inducing fluorescence emission from an analyte; a carrier for positioning the analyte; and a fluorescence detector for receiving the fluorescence, wherein the light irradiator and the fluorescence detector are situated on mutually opposing sides of the carrier, light irradiated from the light irradiator can be passed through to a side where the fluorescence detector is located, and fluorescence emission from the analyte can be induced by the transmitted light while keeping the transmitted light from directly irradiating a fluorescence detecting element of the fluorescence detector. | 2009-03-26 |
| 20090081800 | Selective resonance of chemical structures - Chemical compositions may be selectively or preferentially excited by the application of scores comprising a series of energy inputs. | 2009-03-26 |
| 20090081801 | Process for synthesis of pyrrole derivative, an intermediate for atorvastatin - The present invention also relates to a novel impurity, (6-{2-[2-(6-{2-[2-(4-fluorophenyl)-5-isopropyl-3-phenyl-4-phenylcarbamoyl-pyrrol-1-yl]-ethyl}-2,2-dimethyl-[1,3]-dioxan-4-yl)-acetylamino]-ethyl}-2,2-dimethyl-[1,3]-dioxan-4-yl)-acetic acid tert-butyl ester, the compound of formula IV, having the following structure: | 2009-03-26 |
| 20090081802 | METHOD FOR ENRICHING SHORT-CHAIN NUCLEIC ACIDS - The present invention relates to a method for enriching nucleic acids with a length of not more than 300 nucleotides | 2009-03-26 |
| 20090081803 | POLYVIOLOGEN BORONIC ACID QUENCHERS FOR USE IN ANALYTE SENSORS - The invention relates to a class of glucose-responsive, polyviologen boronic acid quenchers that may be used in combination with fluophores to achieve real-time measurement of glucose levels in vivo. | 2009-03-26 |
| 20090081804 | Monitor and methods for characterizing airborne particulates - A dust monitor is disclosed that is suitably deployed in dusty environments and capable of providing near real-time indications of exposure to airborne particulates. The monitor includes a filter and filter assembly made of materials that do not interfere with subsequent instrumental (such as spectrometric) analysis for detecting and/or quantitating an analyte. In some disclosed embodiments, the filter is made of nylon or other material that is readily subjected to thermal destruction prior to spectrometric analysis. The dust monitor also includes a humidity correction feature that permits the filter to be made of ashable organic materials even if those materials are not highly hydrophobic. Transport devices are provided for shipment of the filter and/or filter assembly to an analytical laboratory which prevent loss of particulate matter and which facilitate an accurate analysis procedure. | 2009-03-26 |
| 20090081805 | Method for finding active ingredients from chemical and biological systems - The present invention relates to a method for finding active ingredients/groups of constitutes from a multi-component system that are active as indicated by specific or a group of activity test. The method involves the use of a hyphenated instrument for determining the active ingredients. | 2009-03-26 |
| 20090081806 | Methods and Compositions for pH Control - The present invention comprises methods and compositions for controlling the pH of a confined body of water, such as a swimming pool, spas, and hot tubs, using an organic acid specifically citric acid alone or in combination with other organic acids, borates or boric acid or other chelants or clarifiers. The amount of organic acid added to the confined body of water is determined relative to the pH of the water prior to such addition and the volume of water being treated. The amount of maintenance doses of organic acid is based upon the volume of water being treated and the magnitude of pH change required for adjustment to desired pH. The organic acid can be dispensed automatically or manually and can be dispensed as a solid, including a dispersable powder form, a capsule, or tablet, or as a liquid concentrate to be diluted in the confined body of water. | 2009-03-26 |
| 20090081807 | METHOD FOR RAPID AND QUANTITATIVE ASSAY USING PRIMARY COLOR PRINCIPLE - A method for rapid and quantitative assay of fluid specimen is disclosed. This method utilizes the principle of primary colors: any color can be discomposed into three primary colors or multiple primary colors (for example, red, green and blue). The molecules of the chemical being measured are bound to three colored substrates at different binding constant. Thus different concentration of the chemical will result in different final colors chromatography imaging. This makes the quantitative assay possible. This method can also be used to detect qualitatively the existence of multiple chemicals simultaneously. | 2009-03-26 |
| 20090081808 | DEVICE AND METHOD FOR IDENTIFYING MYCOTOXINS - The invention relates to an apparatus and a process for detection of mycotoxins and to kits suitable for carrying out said process. | 2009-03-26 |
