10th week of 2022 patent applcation highlights part 29 |
Patent application number | Title | Published |
20220073882 | EXPANSION CULTURE METHOD FOR CARTILAGE OR BONE PRECURSOR CELLS - Culturing a cartilage or bone progenitor cell in a medium containing a TGF-β signal inhibitor and FGF is effective for expansion culture of a cartilage or bone progenitor cell, and inducing differentiation of a cartilage or bone progenitor cell obtained by such a method is effective for producing a chondrocyte or osteocyte. | 2022-03-10 |
20220073883 | COMPOSITIONS AND METHODS FOR GENERATION OF HEART FIELD-SPECIFIC PROGENITOR CELLS - The present invention relates to compositions and methods for producing, identifying and isolating heart progenitor cells. | 2022-03-10 |
20220073884 | SYSTEMS AND METHODS FOR PRODUCING STEM CELLS AND DIFFERENTIATED CELLS - The present invention provides various improved systems and methods for obtaining, generating, culturing, and handling cells, such as stem cells (including induced pluripotent stem cells or iPSCs) and differentiated cells, as well as cells and cell panels produced using such systems and methods, and uses of such cells and cell panels. | 2022-03-10 |
20220073885 | THERAPEUTIC BACTERIOPHAGES - There is provided a bacteriophage of the Myoviridae family, comprising a genome of circularly permuted double-stranded DNA at a length of 200kbp or more; characterised in that the phage has any one or more of the following features: a) does not have a holin gene; and/or b) binds to the | 2022-03-10 |
20220073886 | PURIFICATION OF PROTEINS WITH CATIONIC SURFACTANT - The subject invention provides a method for purifying a target protein from a mixture comprising the target protein and contaminating protein, comprising the steps of exposing the mixture to an effective amount of a cationic surfactant such that the contaminating protein is preferentially precipitated and recovering the target protein. Proteins purified according to the method of the invention are also provided. | 2022-03-10 |
20220073887 | EVOLUTION OF SITE-SPECIFIC RECOMBINASES - Some aspects of the present disclosure provide methods for evolving recombinases to recognize target sequences that differ from the canonical recognition sequences. Some aspects of this disclosure provide evolved recombinases, e.g., recombinases that bind and recombine naturally-occurring target sequences, such as, e.g., target sequences within the human Rosa26 locus. Methods for using such recombinases for genetically engineering nucleic acid molecules in vitro and in vivo are also provided. Some aspects of this disclosure also provide libraries and screening methods for assessing the target site preferences of recombinases, as well as methods for selecting recombinases that bind and recombine a non-canonical target sequence with high specificity. | 2022-03-10 |
20220073888 | COMBINATION TREATMENT - The present invention relates to methods for treating ischemic events in a patient, especially ST-segment elevation myocardial infarction and acute ischemic stroke, by administrating a recombinant apyrase protein in conjunction with a P2Y | 2022-03-10 |
20220073889 | Polypeptide Having Phospholipase C Activity and Use Thereof - Provided is a polypeptide having phospholipase C activity. The polypeptide has: 1) an amino acid sequence represented by SEQ ID No: 2, comprising amino acid substitutions occurred at one or more positions, wherein the one or more positions are selected from positions 6, 8, 10, 104, and 205 in the amino acid sequence represented by SEQ ID No: 2 or any combination thereof; or 2) having at least 80% sequence identity with 1), and at least one of positions 6, 8, 10, 104 and 205 being different from positions 6, 8, 10, 104, and 205 in the amino acid sequence represented by SEQ ID No: 2. Provided are a nucleic acid molecule for encoding the polypeptide, a vector comprising the nucleic acid molecule, and a cell comprising the nucleic acid molecule or the vector. Provided are uses of the polypeptide, the nucleic acid molecule, the vector, and the cell. | 2022-03-10 |
20220073890 | NOVEL CRISPR-CAS SYSTEMS FOR GENOME EDITING - Compositions and methods are provided for genome modification of a target sequence in the genome of a cell, using a novel Cas endonuclease. The methods and compositions employ a guide polynucleotide/endonuclease system to provide an effective system for modifying or altering target sequences within the genome of a cell or organism. Also provided are novel effectors and endonuclease systems and elements comprising such systems, such as guide polynucleotide/endonuclease systems comprising an endonuclease. Compositions and methods are also provided for guide polynucleotide/endonuclease systems comprising at least one endonuclease, optionally covalently or non-covalently linked to, or assembled with, at least one additional protein subunit, and for compositions and methods for direct delivery of endonucleases as ribonucleotide proteins. | 2022-03-10 |
20220073891 | SYSTEMS, METHODS, AND COMPOSITIONS FOR RNA-GUIDED RNA-TARGETING CRISPR EFFECTORS - This disclosure provides systems, methods, and compositions for RNA-guided RNA-targeting CRISPR effectors for the treatment of diseases as well as diagnostics. In some embodiments, nucleotide deaminase functionalized CRISPR systems for RNA editing RNA knockdown, viral resistance, splicing modulation, RNA tracking, translation modulation, and epi-transcriptomic modifications are disclosed. | 2022-03-10 |
20220073892 | Cascade/dCas3 Complementation Assays for In Vivo Detection of Nucleic Acid-Guided Nuclease Edited Cells - The present disclosure relates to methods and compositions that allow one to identify in vivo edited cells when employing nucleic-acid guided editing. Additionally provided are automated multi-module instruments for performing editing and selection methods and using the compositions. | 2022-03-10 |
20220073893 | METHOD FOR REDUCING FRUCTAN IN A FOOD PRODUCT WITH AID OF INVERTASE (EC 3.2.1.26) - The present invention relates to a method for reducing fructan in a fructan-containing food product, comprising adding an invertase belonging to enzyme classification EC 3.2.1.26 to the food product, and incubating the food product with the invertase, wherein fructan in the fructan-containing food product is hydrolysed. The invention further relates to the use of an invertase belonging to enzyme classification EC. 3.2.1.26 for the preparation of a medicament or a dietary supplement for the treatment of a person suffering from irritable bowel syndrome. Invertase (EC 3.2.1.26) may optionally by further combined with inulinase (EC3.2.1.7) and/or beta-fructosidase (EC 3.2.1.80). | 2022-03-10 |
20220073894 | METHODS AND COMPOSITIONS FOR MODIFIED FACTOR IX PROTEINS - Factor IX proteins are described with an increase in the number of glycosylation sites and other modifications to provide Factor IX proteins that have higher specific activity and a longer useful clotting function relative to wild type or non-modified Factor IX protein. | 2022-03-10 |
20220073895 | HUMAN COAGULATION FACTOR VII POLYPEPTIDES - The present invention relates to novel human coagulation Factor VIIa variants having coagulant activity as well as polynucleotide constructs encoding such variants, vectors and host cells comprising and expressing the polynucleotide, pharmaceutical compositions, uses and methods of treatment. | 2022-03-10 |
20220073896 | RECOMBINANT YEAST AND METHOD FOR PRODUCING ETHANOL USING SAME - Provided are excellent L-arabinose metabolic genes that function in yeasts. Provided is an L-arabinose metabolic gene cluster including an L-arabinose isomerase gene specified by a predetermined SEQ ID, an L-ribulokinase gene specified by a predetermined SEQ ID, and an L-ribulose-5-phosphate-4-epimerase gene specified by a predetermined SEQ ID. | 2022-03-10 |
20220073897 | ARTIFICIAL RIBOSOMES FOR FULLY PROGRAMMABLE SYNTHESIS OF NONRIBOSOMAL PEPTIDES - Provided herein, in some embodiments, are artificial ribosomes that synthesize nonribosomal peptides, polyketides, and fatty acids with full control over peptide sequence. Also provided herein are methods for programmed synthesis of nonribosomal peptides, polyketides, and fatty acids. In particular, provided herein are methods for scalable synthesis of a wide range of antibacterial, antifungal, antiviral, and anticancer compounds. | 2022-03-10 |
20220073898 | Tandem Protein Expression - The present invention provides means and methods for improving polypeptide expression yield by increasing the number of polypeptides that can be transported into the endoplasmic reticulum (ER) per translocation event. | 2022-03-10 |
20220073899 | REAGENTS AND METHODS FOR ESTERIFICATION - Methods and reagents for esterification of biological molecules including proteins, polypeptides and peptides. Diazo compounds of formula I: | 2022-03-10 |
20220073900 | CELL ANALYSIS SYSTEMS WITH CELL ENCAPSULATION - In one example in accordance with the present disclosure, a method is described. The method includes receiving, in a microfluidic channel, serially fed cells to be encapsulated. Each cell is individually lysed and a lysate of each cell is transported to a downstream analysis device. The lysate of an individual cell is encapsulated with an encapsulating fluid. | 2022-03-10 |
20220073901 | COMPOSITION FOR EXTENDING TELOMERE OF CELL AND PREPARATION METHOD THEREFOR - Proposed are exosomes for elongating telomeres of cells containing a product of at least one gene selected from among TERT, TERF2, DKC1, TERF2IP, RFC1, RAD50, TERF1, PINX1, TNKS1BP1, ACD, NBN, HSPA1L, PARP1, PTGES3, SMG6, BLM, XRCC5, XRCC6, ERCC4, PRKDC, TEP1 and β-catenin, a composition containing the same, and a method of inducing telomere-elongating exosomes from cells by providing physical stimulation directly or indirectly to the cells. The composition containing the exosomes may exhibit its effects even when it is applied to a living body in a noninvasive manner at a lower concentration than a previously disclosed composition, and has high safety. | 2022-03-10 |
20220073902 | METHODS OF CAPTURING CELL-FREE METHYLATED DNA AND USES OF SAME - There is described herein, a method of capturing cell-free methylated DNA from a sample having less than 100 mg of cell-free DNA, comprising the steps of: subjecting the sample to library preparation to permit subsequent sequencing of the cell-free methylated DNA; adding a first amount of filler DNA to the sample, wherein at least a portion of the filler DNA is methylated; denaturing the sample; and capturing cell-free methylated DNA using a binder selective for methylated polynucleotides. | 2022-03-10 |
20220073903 | METHODS OF PURIFYING RIBONUCLEIC ACID SPECIES - The present disclosure is directed to ribonucleic acid (RNA) isolation and purification. For example, the present disclosure relates to a method of purifying a single ribonucleic acid (RNA) species, including: isolating a DNA nanoswitch-target complex within a gel medium, wherein the DNA nanoswitch-target complex includes a DNA nanoswitch and a target-of-interest; digesting the DNA nanoswitch and the gel medium to form digested byproducts, and a free target-of-interest; and isolating the free target-of-interest, wherein the free target-of-interest is a single RNA species. | 2022-03-10 |
20220073904 | DEVICES AND METHODS FOR DISPLAY OF ENCODED PEPTIDES, POLYPEPTIDES, AND PROTEINS ON DNA - A novel method for displaying proteins and peptides is disclosed in which individual proteins or peptides remain associated with the DNA encoding them. Proteins or peptides can be generated by in vitro translation of DNA templates, either free in solution or arrayed on a solid support, such that the proteins or peptides remain immobilized on their DNA templates. In particular, high throughput sequencing can be combined with high throughput functional characterization of encoded proteins and peptides, wherein the identity of each protein or peptide is determined by DNA sequencing, and functional studies are carried out directly on each protein or peptide while immobilized on the DNA template encoding it. The methods of the invention should find numerous applications, for example, in high throughput genetic or pharmacological screening, epitope mapping, and protein engineering and directed evolution. | 2022-03-10 |
20220073905 | METHODS AND MATERIALS FOR SINGLE CELL TRANSCRIPTOME-BASED DEVELOPMENT OF AAV VECTORS AND PROMOTERS - This document provides a high throughput method for the creation of AAV vectors and/or promoter sequences with high efficiency and/or specificity for multiple cell types. | 2022-03-10 |
20220073906 | ADAPTORS AND METHODS FOR HIGH EFFICIENCY CONSTRUCTION OF GENETIC LIBRARIES AND GENETIC ANALYSIS - The disclosure provides compositions and methods for the multiplexed detecting and analyzing of cellular nucleic acids. In some embodiments, the disclosure provides multifunctional adaptors for use in methods of the disclosure. In some embodiments, compositions and methods of the disclosure are automatable. | 2022-03-10 |
20220073907 | TAGLESS ENCODED CHEMICAL LIBRARY - Described is a method for screening an encoded chemical library, which library comprises a plurality of different chemical structures each releasably linked to an encoding tag, the method comprising the steps of: (a) providing said library of tagged chemical structures; (b) releasing each chemical structure from its tag to produce a plurality of free, tagless chemical structures (TCSs); (c) screening the TCSs by contacting them with a assay system under conditions whereby a spatial association between each TCS and its tag is maintained, to produce a plurality of different screened TCSs each spatially associated with its tag; and (d) identifying a screened TCS by decoding a tag that is spatially associated therewith. | 2022-03-10 |
20220073908 | METHODS OF PRODUCING HIGH DIVERSITY PEPTIDE LIBRARIES AND PROMOTING PROTEIN FOLDING - The disclosure provides a peptide library with increased peptide diversity. The increase in peptide diversity can occur via cleavage of particular amino acids within a peptide. The disclosure further provides a method for promoting folding of a peptide into an active conformation. | 2022-03-10 |
20220073909 | METHODS AND COMPOSITIONS FOR RAPID NUCLEIC LIBRARY PREPARATION - Rapid nucleic acid libraries, methods of generation, kits, and compositions relating to library synthesis, including reagents, intermediaries and final products are disclosed herein. The disclosure enables rapid synthesis of libraries that allow independent verification of sequence information and rapid identification of sequence information with template of origin. | 2022-03-10 |
20220073910 | A Cross-Linking Approach to Map Small Molecule-RNA Binding Sites in Cells - Disclosed herein are compounds and methods to identify the direct RNA targets of small molecules in cells is described. The approach, dubbed Chemical Cross-Linking and Isolation by Pull-down to Map Small Molecule-RNA Binding Sites (Chem-CLIP-Map-Seq), appends a cross-linker and a purification tag onto a small molecule. In cells, the compound binds to RNA and undergoes a proximity-based reaction. The cross-linked RNA is purified and then amplified using a universal reverse transcription (RT) primer and gene-specific PCR primers. At nucleotides proximal to the binding site, RT “stops” are observed. This approach has broad utility in identifying and validating the RNA targets and binding sites of small molecules in the context of a complex cellular system. | 2022-03-10 |
20220073911 | METHODS FOR APTAMER SELECTION - The present disclosure relates to methods for identifying aptamers against allergen proteins and signaling polynucleotides (SPNs) for allergen detection. The screening method of the present disclosure combines several positive SELEX selections, and on-chip positive and counter selections to identify aptamer sequences that are preferentially bind to target proteins when competing with short complementary sequences. | 2022-03-10 |
20220073912 | Systems and Methods for Modulating CRISPR Activity - Provided herein are polynucleotides comprising sequence configured to bind to a CRISPR effector protein. Modulation of one or more modifications of the polynucleotides can be used to tune the activity of CRISPR effector proteins complexed with the polynucleotides. | 2022-03-10 |
20220073913 | METHODS AND COMPOSITIONS FOR MODULATING ALPHA-1-ANTITRYPSIN EXPRESSION - Disclosed herein are methods for decreasing A1AT mRNA and protein expression and treating, ameliorating, preventing, slowing progression, or stopping progression of fibrosis. Disclosed herein are methods for decreasing A1AT mRNA and protein expression and treating, ameliorating, preventing, slowing progression, or stopping progression of liver disease, such as, A1ATD associated liver disease, and pulmonary disease, such as, A1ATD associated pulmonary disease in an individual in need thereof. Methods for inhibiting A1AT mRNA and protein expression can also be used as a prophylactic treatment to prevent individuals at risk for developing a liver disease, such as, A1ATD associated liver disease and pulmonary disease, such as, A1ATD associated pulmonary disease. | 2022-03-10 |
20220073914 | COMPOUNDS AND METHODS FOR MODULATION OF SMN2 - Disclosed herein are compounds, compositions and methods for modulating splicing of SMN2. Also provided are uses of disclosed compounds and compositions in the manufacture of a medicament for treatment of spinal muscular atrophy. | 2022-03-10 |
20220073915 | ARTIFICIAL NUCLEIC ACIDS FOR RNA EDITING - The present invention concerns artificial nucleic acids for site-directed editing of a target RNA. In particular, the present invention provides artificial nucleic acids capable of site-directed editing of endogenous transcripts by harnessing an endogenous deaminase. Further, the present invention provides artificial nucleic acids for sited-directed editing of a target RNA, which are chemically modified, in particular according to a modification pattern as described herein. The invention also comprises a vector encoding said artificial nucleic acid and a composition comprising said artificial nucleic acid. Moreover, the invention provides the use of the artificial nucleic acid, the composition or the vector for site-directed editing of a target RNA or for in vitro diagnosis. In addition, the artificial nucleic acid, the composition or the vector as described herein are provided for use as a medicament or for use in diagnosis of a disease or disorder. | 2022-03-10 |
20220073916 | NOVEL PHOSPHORAMIDITES - The invention relates to a compound of formula (II) | 2022-03-10 |
20220073917 | U1 snRNP Regulates Gene Expression and Modulates Oncogenicity - The invention provides a method of regulating U1 activity associated with its splicing role as well as its role in protecting pre-mRNAs from premature termination by cleavage and polyadenylation, thereby modulating expression of a gene or genes. In one embodiment, the invention includes compositions and methods for regulating gene expression and treating diseases associated with dysregulated gene expression. | 2022-03-10 |
20220073918 | ALLELE SELECTIVE INHIBITION OF MUTANT C9ORF72 FOCI EXPRESSION BY DUPLEX RNAS TARGETING THE EXPANDED HEXANUCLEOTIDE REPEAT - Provided herein are compositions and methods for reducing expression of C9orf72 transcripts in cells containing expanded intronic GGGGCC regions, including those in subjects having or at risk of developing amyotrophic lateral sclerosis (ALS) and frontotemporal dementia (FTD). | 2022-03-10 |
20220073919 | THERAPEUTIC OLIGONUCLEOTIDES - Methods and compositions are provided for oligonucleotides that bind targets of interest. The targets include cells and microvesicles, such as those derived from various diseases. The oligonucleotides can be used for diagnostic and therapeutic purposes. The target of the oligonucleotides can be a therapeutic target such as Complement Component 1, Q Subcomponent (C1q) or a subunit thereof. | 2022-03-10 |
20220073920 | DNA APTAMERS SPECIFIC OF ADENOVIRUS TYPES - A single stranded nucleic acid aptamer able to specifically bind to at least one Adenovirus type, the aptamer comprising or consisting of one of sequences SEQ ID NO:1 to 3, or variants thereof having at least 50% sequence identity. Additionally, a composition comprising the aptamer. Furthermore, use of the aptamer, for detecting, capturing, concentrating and/or quantifying at least one Adenovirus type. Still further, in vitro methods for capturing, detecting and/or quantifying at least one Adenovirus type. Further yet, a kit for detecting, quantifying, capturing and/or concentrating at least one Adenovirus type. | 2022-03-10 |
20220073921 | APTAMER AND USE OF THE APTAMER IN THE DIAGNOSIS AND TREATMENT OF CANCER - The present invention relates to an aptamer comprising a nucleotide sequence SEQ ID NO: 1 or a fragment thereof of at least 20 contiguous nucleotides of the nucleotide sequence SEQ ID NO: 2, wherein the aptamer comprises a polyethylene glycol (PEG) moiety conjugated to the 5′ or the 3′ end. The invention further relates to a composition comprising the aptamer, and the use of the aptamer in the diagnosis and treatment of cancer, particularly hormone refractory prostate tumours. | 2022-03-10 |
20220073922 | Aptamers and use thereof - The present disclosure provides an anti-Ang2 aptamer, a bispecific molecule or a composition comprising the anti-Ang2 aptamer, as well as uses thereof. | 2022-03-10 |
20220073923 | METHODS AND COMPOSITIONS USING RNA INTERFERENCE AND ANTISENSE OLIGONUCLEOTIDES FOR INHIBITION OF KRAS - The invention relates to the inhibition of expression of mutant KRAS sequences using RNA interference, antisense oligonucleotides, and chemically-modified oligonucleotides. | 2022-03-10 |
20220073924 | ANGIOPOIETIN-LIKE 3 (ANGPTL3) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ANGPTL3 gene, as well as methods of inhibiting expression of ANGPTL3 and methods of treating subjects having a disorder of lipid metabolism, such as hyperlipidemia or hypertriglyceridemia, using such dsRNA compositions. | 2022-03-10 |
20220073925 | ANGIOPOIETIN-LIKE 3 (ANGPTL3) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ANGPTL3 gene, as well as methods of inhibiting expression of ANGPTL3 and methods of treating subjects having a disorder of lipid metabolism, such as hyperlipidemia or hypertriglyceridemia, using such dsRNA compositions. | 2022-03-10 |
20220073926 | ANGIOPOIETIN-LIKE 3 (ANGPTL3) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ANGPTL3 gene, as well as methods of inhibiting expression of ANGPTL3 and methods of treating subjects having a disorder of lipid metabolism, such as hyperlipidemia or hypertriglyceridemia, using such dsRNA compositions. | 2022-03-10 |
20220073927 | ANGIOPOIETIN-LIKE 3 (ANGPTL3) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ANGPTL3 gene, as well as methods of inhibiting expression of ANGPTL3 and methods of treating subjects having a disorder of lipid metabolism, such as hyperlipidemia or hypertriglyceridemia, using such dsRNA compositions. | 2022-03-10 |
20220073928 | ANGIOPOIETIN-LIKE 3 (ANGPTL3) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ANGPTL3 gene, as well as methods of inhibiting expression of ANGPTL3 and methods of treating subjects having a disorder of lipid metabolism, such as hyperlipidemia or hypertriglyceridemia, using such dsRNA compositions. | 2022-03-10 |
20220073929 | ANGIOPOIETIN-LIKE 3 (ANGPTL3) iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to double-stranded ribonucleic acid (dsRNA) compositions targeting the ANGPTL3 gene, as well as methods of inhibiting expression of ANGPTL3 and methods of treating subjects having a disorder of lipid metabolism, such as hyperlipidemia or hypertriglyceridemia, using such dsRNA compositions. | 2022-03-10 |
20220073930 | COMPOSITIONS AND METHODS FOR TREATING AND PREVENTING AMYOTROPHIC LATERAL SCLEROSIS - Dosage regimens for SOD1-targeting antisense oligonucleotides, and salts thereof, are provided. These dosage regimens find use in the treatment of subjects having or at risk of developing amyotrophic lateral sclerosis. | 2022-03-10 |
20220073931 | ANTISENSE OLIGONUCLEOIDES OF GLUTATHIONE S-TRANSFERASES FOR CANCER TREATMENT - The present invention relates to the identification of glutathione S transferase in tumors containing the same to be treated to inhibit protein expression of GSTs proteins and induce cell death and decrease tumor volume. | 2022-03-10 |
20220073932 | ANTISENSE OLIGONUCLEOTIDES FOR ALLELE SPECIFICITY - The present invention provides an antisense oligonucleotide compound 17 to 25 nucleotides in length comprising at least 12 contiguous nucleobases complementary to an equal length portion of a target RNA sequence, wherein the antisense oligonucleotide compound comprises a 3′ domain and a 5′ domain, wherein the 3′ domain is 10 to 12 nucleotides in length and each nucleotide comprises a deoxyribonucleotide and a phosphodiester or phosphothioate internucleotide linkage or combinations thereof, and wherein the 5′ domain is 5 to 15 nucleotides in length, and wherein the 5′ domain comprises unmodified deoxyribonucleotides, unmodified ribonucleotides, modified deoxyribonucleotides, modified ribonucleotides, or combinations thereof, provided that the 5′ domain comprises at least 1 modified deoxyribonucleotide or modified ribonucleotide comprising a modified sugar and/or backbone. The invention further provides methods of using the antisense oligonucleotide compounds as described herein. | 2022-03-10 |
20220073933 | METHODS AND COMPOSITIONS FOR INCREASING PROTEIN EXPRESSION AND/OR TREATING A HAPLOINSUFFICIENCY DISORDER - A tRNA that hybridizes to a non-optimal codon can be used to increase expression in a mammalian cell of a gene product encoded by a gene containing the non-optimal codon or to treat a haploinsufficiency disorder in a subject having a haploinsufficient gene containing the non-optimal codon. | 2022-03-10 |
20220073934 | Beta-Galactosidase Alpha Peptide as a Non-Antibiotic Selection Marker and Uses Thereof - Provided herein are methods of using a nucleic acid construct as a selectable marker. The nucleic acid construct comprises an isolated β-galactosidase expression cassette comprising a nucleic acid sequence encoding the amino-terminal fragment of β-galactosidase operably linked to a promoter. Also provided are isolated vectors comprising the β-galactosidase expression cassette, methods of generating the isolated vector, and kits comprising the isolated vector. | 2022-03-10 |
20220073935 | Novel Cell-Based Assay for Determining Activity in the Retinoblastoma Pathway - Disclosed are methods of determining activity of CDK4 and CDK6 variants upon exposure to CDK inhibitors, methods for determining activity of a Rb variant, methods for determining the activity of a p16 variant in a cell, and methods for determining the sensitivity of a CDK4 variant or a CDK6 variant to p16 in a cell. Stable cell lines for determining activity of CDK4 variants, CDK6 variants, Rb variants, and p16 variants are also disclosed. | 2022-03-10 |
20220073936 | PRE-CONDITIONING TREATMENTS TO IMPROVE PLANT TRANSFORMATION - Provided herein are methods for increasing plant cell transformation efficiency. These methods include exposing the plant cells to a liquid medium containing a surfactant. Following exposure to the surfactant-containing medium, the cells can become more amenable to transformation and may be genetically transformed using methods known in the art. Exposure of the cells to the surfactant-containing medium prior to transformation can increase plant transformation efficiency when compared to transformation efficiency of cells not exposed to the surfactant-containing medium. | 2022-03-10 |
20220073937 | INCREASING GENE EDITING AND SITE-DIRECTED INTEGRATION EVENTS UTILIZING MIEOTIC AND GERMLINE PROMOTERS - This disclosure provides methods and compositions for increasing genome editing and site-directed integration events utilizing guided endonucleases and meiotic cell-preferred, egg cell-preferred or embryo tissue-preferred promoters. | 2022-03-10 |
20220073938 | WHEAT-MALE STERILITY GENE WMS AND ITS ANTHER-SPECIFIC EXPRESSION PROMOTER AND USES THEREOF - The present invention provides a novel gene WMS conferring wheat male sterility, its anther-specific expression promoter, and uses of the same. In wheat, a well-known gene Ms2 causing dominant male sterility has been widely applied in recurrent selection in China. A RNA-seq approach was performed to reveal the anther-specific transcriptome in a pair of Ms2 isogenic lines, ‘Lumai 15’ and ‘Lumai 15+Ms2’. As a result, a WMS gene was identified showing anther-specific expression at the early stage of meiosis and only in wheat carrying the Ms2 gene. The regulation of WMS could alter plant male fertility. The promoter of WMS was found to comprise anther-specific activity. Thus, the present invention might be used to achieve anther-specific gene expression, to develop male sterility in various plant species, to establish recurrent selection in various plant species, and to assist hybrid seed production. | 2022-03-10 |
20220073939 | MODULATING LIGHT RESPONSE PATHWAYS IN PLANTS, INCREASING LIGHT-RELATED TOLERANCES IN PLANTS, AND INCREASING BIOMASS IN PLANTS - Methods and materials for modulating low light and/or shade tolerance, and red light specific responses in plants are disclosed. For example, nucleic acids encoding low light and/or SD+EODFR-tolerance polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased low light and/or SD+EODFR tolerance. In addition, methods and materials involved in increasing UV-B tolerance in plants and methods and materials involved in modulating biomass levels in plants are provided. | 2022-03-10 |
20220073940 | MODULATING LIGHT RESPONSE PATHWAYS IN PLANTS, INCREASING LIGHT-RELATED TOLERANCES IN PLANTS, AND INCREASING BIOMASS IN PLANTS - Methods and materials for modulating low light and/or shade tolerance, and red light specific responses in plants are disclosed. For example, nucleic acids encoding low light and/or SD+EODFR-tolerance polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased low light and/or SD+EODFR tolerance. In addition, methods and materials involved in increasing UV-B tolerance in plants and methods and materials involved in modulating biomass levels in plants are provided. | 2022-03-10 |
20220073941 | HERBICIDE-RESISTANT CAMELINA SATIVA PLANTS, AND VARIANT CAMELINA ACETOHYDROXYACID SYNTHASE POLYPEPTIDES - Provided are variants of the | 2022-03-10 |
20220073942 | Cells with reduced inhibitor production and methods of use thereof - The invention relates to a method of cell culture where the cells are modified to reduce the level of synthesis of growth and/or productivity inhibitors by the cell. The invention also relates to a method of cell culture for improving cell growth and productivity, in particular in culture of mammalian cells at high cell density. The invention further relates to a method of producing cells with improved cell growth and/or productivity in cell culture and to cells obtained or obtainable by such methods. | 2022-03-10 |
20220073943 | LIVER-SPECIFIC VIRAL PROMOTERS AND METHODS OF USING THE SAME - The present invention relates to promoters that function specifically or preferentially in the liver. These promoters are capable of enhancing liver-specific expression of genes. The invention also relates to expression constructs, vectors and cells comprising such liver-specific promoters, and to methods of their use. The present invention future relates to adeno-associated virus (AAV) gene therapy vectors comprising the liver-specific promoters, therapeutic agents comprising the liver-specific promoters, and methods using the same. | 2022-03-10 |
20220073944 | MULTIMERIC CODING NUCLEIC ACID AND USES THEREOF - The present invention provides, among other things, multimeric coding nucleic acids that exhibit superior stability for in vivo and in vitro use. In some embodiments, a multimeric coding nucleic acid (MCNA) comprises two or more encoding polynucleotides linked via 3′ ends such that the multimeric coding nucleic acid compound comprises two or more 5′ ends. | 2022-03-10 |
20220073945 | EXPRESSION VECTORS FOR EUKARYOTIC EXPRESSION SYSTEMS - The invention provides expression vectors for expressing multi-chain recombinant proteins (e.g., biologics) in mammalian cells. Also provided are host cells comprising the expression vectors, methods of producing the multi-chain recombinant proteins, and methods of propagating the expression vectors. | 2022-03-10 |
20220073946 | VIRUS-LIKE PARTICLES OF CMV MODIFIED BY FUSION - The present invention relates to a modified virus-like particle (VLP) of cucumber mosaic virus (CMV) comprising at least one fusion protein, wherein said at least one fusion protein comprises, or preferably consists of b) a chimeric CMV polypeptide, wherein said chimeric CMV polypeptide comprises, or preferably consists of (iii) a CMV polypeptide, wherein said CMV polypeptide comprises, or preferably consists of, a coat protein of CMV, wherein preferably said coat protein of CMV comprises, or preferably consists of, SEQ ID NO:62; or an amino acid sequence having a sequence identity of at least 75%, preferably of at least 80%, more preferably of at least 85%, again further preferably of at least 90%, again more preferably of at least 95%, still further preferably of at least 98% and still again further more preferably of at least 99% with SEQ ID NO:62; and (iv) an antigenic polypeptide, wherein said antigenic polypeptide is inserted into said CMV polypeptide, wherein said insertion of said antigenic polypeptide is between amino acid residues of said CMV polypeptide corresponding to amino acid residues of position 84 and position 85 of SEQ ID NO:62; and (iii) a T helper cell epitope, wherein said T helper cell epitope replaces a N-terminal region of said CMV polypeptide, and wherein preferably said N-terminal region of said CMV polypeptide corresponds to amino acids 2-12 of SEQ ID NO:62. | 2022-03-10 |
20220073947 | AAV-BASED CONDITIONAL EXPRESSION SYSTEM - The present invention relates to a cell comprising (aa) a nucleic acid comprising in 5′ to 3′ direction (i) at least one adeno-associated virus (AAV) inverted terminal repeat (ITR) sequence; (ii) a promoter which is capable of being activated by (a) helper polypeptide(s) and optionally (a) helper polynucleotide(s); and (iii) a transgenic coding sequence under the control of said promoter of (aa)(ii); and (ab) a nucleic acid comprising in 5′ to 3′ direction (i) a promoter which is capable of being activated by said helper polypeptide(s) and optionally said helper polynucleotide(s); and (ii) at least one AAV rep gene coding sequence under the control of said promoter of (ab)(i); wherein said cell does not comprise an AAV cap gene and/or is not able to express any AAV cap gene product. | 2022-03-10 |
20220073948 | ADENOVIRAL-BASED BIOLOGICAL DELIVERY AND EXPRESSION SYSTEM FOR USE IN THE TREATMENT OF OSTEOARTHRITIS - The invention relates to an adenoviral-based biological delivery and expression system for use in the treatment or prevention of osteoathritis in human or mammalian joints by long-term inducible gene expression of human or mammalian interleukin-1 receptor antagonist (II-1 Ra) in synovial cells, comprising a helper-dependent adenoviral vector containing a nucleic acid sequence encoding for human or mammalian interleukin-1 receptor antagonist (II-1 Ra), left and right inverted terminal repeats (L ITR and R ITR), the adenoviral packaging signal and non-viral, non-coding stuffer nucleic acid sequences, wherein the expression of the human or mammalian interleukin-1 receptor antagonist (II-1 Ra) gene within synovial cells is regulated by an inflammation-inducible promoter. | 2022-03-10 |
20220073949 | ELECTROPORATION MODULES AND INSTRUMENTATION - The present disclosure provides a sphere-packing lattice electroporation device configured for use as a stand-alone unit or in an automated multi-module cell processing environment and configured to decrease cell processing time and cell survival. The sphere-packing lattice utilizes lattice-forming beads that are uniform in size and that self-assemble into a crystalline-like lattice. | 2022-03-10 |
20220073950 | ANELLOSOMES FOR DELIVERING PROTEIN REPLACEMENT THERAPEUTIC MODALITIES - This invention relates generally to anellosomes and compositions and uses thereof. | 2022-03-10 |
20220073951 | SYSTEMS AND METHODS FOR THE TREATMENT OF HEMOGLOBINOPATHIES - Genome editing systems, guide RNAs, and CRISPR-mediated methods are provided for altering portions of the HBG1 and HBG2 loci in cells and increasing expression of fetal hemoglobin. | 2022-03-10 |
20220073952 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2022-03-10 |
20220073953 | SYNTHETIC ENZYME COMPLEXES FOR IN VITRO RUBBER PRODUCTION - Disclosed herein are recombinant enzymes derived from various rubber-producing plants and the use of those enzymes in the production of natural rubber outside of plant tissues. Systems and methods for the utilization of these enzymes in the production of natural rubber are also provided. | 2022-03-10 |
20220073954 | HYBRID YEAST WITH INCREASED ETHANOL PRODUCTION - Described are compositions and methods related to hybrid yeast that produces an increased amount of ethanol from starch-containing substrates compared to its parental yeast. Such yeast is well-suited for use in fuel alcohol production to increase yield. | 2022-03-10 |
20220073955 | METHODS FOR PRODUCTION OF OXYGENATED TERPENES - The present disclosure relates to methods for producing oxygenated terpenoids, and preparation of compositions and formulations thereof. Polynucleotides, derivative enzymes, and host cells for use in such methods are also provided. | 2022-03-10 |
20220073956 | SYSTEMS AND METHODS FOR RECYCLING OF REDUCED DENSITY BIOPLASTICS - A method for deriving value from a mixed waste feedstock can include receiving a mixed waste feedstock including at least a reduced density biopolymer material and an organic feedstock. At least one of a fluid or a material that releases liquids during degradation is added to the mixed waste feedstock. The reduced density biopolymer material is separated, via density separation, from the mixed waste feedstock. The reduced density biopolymer material has a specific gravity below a specific gravity threshold. The reduced density biopolymer material separated from the mixed waste feedstock as a result of the separating is recovered. | 2022-03-10 |
20220073957 | Processes and Systems for Catalytic Manufacture of Wax Ester Derivatives - Processes for transesterifying wax esters. Implementations may include: providing a feedstock including wax esters, contacting the feedstock with a lipase, and catalytically transesterifying the wax esters in the feedstock with the lipase to form a transesterified product. An oxidative stability index (OSI) of the transesterified product may be greater than an OSI of the feedstock. | 2022-03-10 |
20220073958 | SINGLE STEP BIOCATALYTIC AMIDATION - The present invention relates to a process for biocatalytic amidation of non-protected amino acids comprising the step of contacting a non-protected amino acid with an ammonia source in the presence of an organic solvent and an enzyme. | 2022-03-10 |
20220073959 | METHODS OF GANGLIOSIDE PRODUCTION - The invention provides methods for production of gangliosides, e.g., GM1, from cells in culture using, for example, bone marrow cells and neuroblastoma cells. Methods include the treatment of cells with neural induction media and chloroquine, or chloroquine alone in the case of, e.g., human bone marrow cells, neuraminidase or glucosamine, to induce the production of gangliosides, e.g. GM1, in the cells. Also provided are methods of long-term, high density culturing of cells without passaging to produce gangliosides, e.g., GM1. Methods of quantifying gangliosides, e.g., GM1 in cell culture are also provided. | 2022-03-10 |
20220073960 | Recombinant Production of Steviol Glycosides - Recombinant microorganisms, plants, and plant cells are disclosed that have been engineered to express novel recombinant genes encoding steviol biosynthetic enzymes and UDP-glycosyltransferases (UGTs). Such microorganisms, plants, or plant cells can produce steviol or steviol glycosides, e.g., rubusoside or Rebaudioside A, which can be used as natural sweeteners in food products and dietary supplements. | 2022-03-10 |
20220073961 | MULTILAYER ELECTROCHEMICAL ANALYTE SENSORS AND METHODS FOR MAKING AND USING THEM - Embodiments of the invention provide multilayer analyte sensors having material layers (e.g. high-density amine layers) and/or configurations of material layers that function to enhance sensor function, as well as methods for making and using such sensors. Typical embodiments of the invention include glucose sensors used in the management of diabetes. | 2022-03-10 |
20220073962 | METHODS FOR RNA ANALYSIS - The present invention is concerned with methods for analyzing RNA molecules. The provided methods involve conjugates for RNA cleavage comprising a chemical moiety with RNA cleaving activity and an oligonucleotide. The oligonucleotide is designed based on a target sequence present in an RNA molecule, and the cleavage of the RNA molecule is inter alia carried out at conditions allowing the hybridization of the oligonucleotide to the target 5 sequence. Thereby, the method is easily applicable to RNA molecules of any sequence. The method further involves the analysis of the RNA fragments obtained after cleavage to obtain information on the physical properties of the RNA molecule. | 2022-03-10 |
20220073963 | COMPOSITIONS AND METHODS FOR DETECTING AND TREATING TYPE 1 DIABETES AND OTHER AUTOIMMUNE DISEASES - The present invention relates to the field of diabetes. More specifically, the present invention provides compositions and methods useful for diagnosing and treating Type I diabetes. In one embodiment, a method comprises detecting a nucleotide sequence encoding SEQ ID NO:1 from a biological sample obtained from a patient. In another embodiment, the present invention provides an antibody or antigen-binding fragment thereof that specifically binds SEQ ID NO:1. In a further embodiment, the present invention provides an antibody or antigen-binding fragment thereof that specifically binds (i) a B-cell receptor expressed on a lymphocyte, wherein the B-cell receptor comprises SEQ ID NO:1; or (ii) a free-floating antibody comprising SEQ ID NO:1. | 2022-03-10 |
20220073964 | NUCLEIC ACID DETECTION - The present disclosure relates to nucleic acid detection devices. The nucleic detection device can include a microfluidic architecture to receive biological fluid, a multimodal sensor bundle, a common transmission line to transmit an enhanced current signal generated by combining current signals from individual sensors, and a current to voltage converter to receive and convert the enhanced current signal to voltage. The multimodal sensor bundle can be positioned to interact with the biological fluid when present within the microfluidic architecture. The multimodal sensor bundle can include multiple types of sensors selected from an electrochemical sensor, an optical sensor, or a potentiometric sensor. Individual sensors of the multimodal sensor bundle independently can generate a current signal in response to interaction with the biological fluid. | 2022-03-10 |
20220073965 | METHOD FOR QUICKLY EXTRACTING LONG-FRAGMENT GENOMIC DNA BY SINGLE REACTION TUBE, AND KIT - A method for quickly extracting long-fragment genomic DNAs by a single reaction tube, comprising the following steps: a) adding a lysis solution and protease K to a sample, to lyse cells and release the genomic DNAs; b) adding a precipitant to the reaction system of step a) to obtain a precipitate of the genomic DNAs; c) washing the obtained precipitate of the genomic DNAs using a washing solution; and d) dissolving the genomic DNAs using a dissolution solution. Also provided is a kit suitable for the method above. | 2022-03-10 |
20220073966 | VIAL CAPS FOR BIOLOGICAL PROCESSING OR ANALYSIS - Provided herein are vial caps for use with assay vials, tubes, or plates. The vial caps can be compatible with various analytic devices, for example, thermocycler. The vial caps can be used with polymerase chain reaction (PCR) assay vials, tubes, or plates in any thermocycling reactions. The vial caps described herein can prevent evaporation during thermocycling reactions. | 2022-03-10 |
20220073967 | PROCESS FOR THE ENZYMATIC SYNTHESIS AND AMPLIFICATION OF NUCLEIC ACIDS - A method for amplification of nucleic acids in which substantially use is made of the fact that a pre-defined nucleic acid chain (target sequence) can be multiplied/amplified in the presence of a target sequence-specific activator oligonucleotide. The target sequence-specific activator oligonucleotide causes the separation of re-synthesized complementary primer extension products by strand displacement, so that a new primer oligonucleotide can attach to the respective template strand. The thus formed complex of a primer oligonucleotide and a template strand can initiate a new primer extension reaction. The thus formed primer extension products in turn function as templates, so that an exponential amplification reaction results. Amplification of a particular target sequence takes place more efficiently in case of perfect match complemetary base pair formation between the activator oligonucleotide and the corresponding target sequence. Mismatches between the activator oligonucleotide and a particular target sequence can result in less efficient amplification. The efficiency of synthesis of perfect match target sequences and mismatch sequences can be measured and compared. | 2022-03-10 |
20220073968 | METHODS FOR IDENTIFYING MICROBIAL STRAINS HAVING ENHANCED PLANT COLONIZATION EFFICIENCY - Methods of evaluating microorganisms to determine efficiency of colonization of a plant or plant part are described. Methods of identifying genetic elements correlated with colonization efficiency of plant-associated microorganism are also provided. Methods to identify microorganisms for use as inoculants for improved plant yield using the colonization screening methods or the presence of genetic elements associated with colonization efficiency are described. Further methods useful for identification of microorganisms useful as inoculants for improving plant yield are presented. | 2022-03-10 |
20220073969 | USE OF SIDEROMYCINS TO LIMIT CROSS-REACTIVITY AND IMPROVE SPECIES IDENTIFICATION IN ANTIBIOTIC SUSCEPTIBILITY ASSAYS - The present invention relates to the use of sideromycins as additives in non-replicative transduction particles based systems either to limit cross-reactivity of unwanted organisms or to identify the organism being run on an antibiotic susceptibility assay (AST assay). Addition of the sideromycins removes or reduces light production from bacteria that are sensitive to them, allowing for prevention of cross-reactivity in AST assays and/or family, genus, and potentially species level bacteria strain identification when performing AST testing. | 2022-03-10 |
20220073970 | MONITORING AND DIAGNOSTIC METHODS FOR FELINE MICROBIOME CHANGES - Methods for assessing the microbiome of a feline are provided. The methods include, inter alia, detecting one or more bacterial species in a sample obtained from the feline, comparing the one or more bacterial species to a control data set, and determining the microbiome age. | 2022-03-10 |
20220073971 | METHOD FOR ANALYZING MRNA PRECURSOR, INFORMATION PROCESSING APPARATUS, AND COMPUTER PROGRAM - Provided is a technology involving analyzing the state of mRNA of a eukaryote in a simple manner, and facilitating, for example, the proposal of a measure in the event that the state is an unpreferred state. That is, provided is an analysis method for a precursor mRNA, including quantitatively analyzing a removal state of an intron in a precursor mRNA of a eukaryote and outputting information specific to the eukaryote. The presence or absence of an aged-type or presymptomatic disease-type splicing pattern different from a healthy-type splicing pattern is detected from a quantitatively analyzed removal state of the intron. From a pre-made substance list, information on a pharmaceutically acceptable substance for bringing the aged-type or presymptomatic disease-type splicing pattern closer to the healthy-type splicing pattern is identified. The identified information is output as information specific to the eukaryote serving as an analysis object. | 2022-03-10 |
20220073972 | METHOD FOR SIMULTANEOUSLY DETECTING EXOSOME MEMBRANE PROTEIN AND MRNA - A method for simultaneously detecting an exosome membrane protein and mRNA is provided. The method can perform simultaneous detection on exosomes separated and purified from the same sample by labeling an exosome membrane protein with a fluorescent antibody and labeling a target gene mRNA with a molecular beacon, wherein labeling the exosome with the molecular beacon is specifically performed by means of an in-situ exosome capture well plate or chip, and each well or chip in the in-situ exosome capture well plate includes a fluorescein-labeled molecular beacon; and the molecular beacon is a specific DNA probe for detecting the target gene mRNA. The present invention utilizes an in-situ exosome capture well plate or chip technology to detect a biomarker gene mRNA contained in an exosome of a biological sample. | 2022-03-10 |
20220073973 | METHODS FOR NUCLEIC ACID SEQUENCING - Methods of sequencing molecules based on luminescence lifetimes and/or intensities are provided. In some aspects, methods of sequencing nucleic acids involve determining the luminescence lifetimes, and optionally luminescence intensities, of a series of luminescently labeled nucleotides incorporated during a nucleic acid sequencing reaction. | 2022-03-10 |
20220073974 | METHODS AND DEVICES FOR SPATIALLY ENCODED BIOLOGICAL ASSAYS - The present disclosure generally relates to methods for increasing the region of interest of spatial multi-omics techniques while retaining single-cell resolution. These methods can improve the scaling of region of interest dimension with input/output channels from linear to super-linear. In some embodiments, the method is performed by providing a plurality of probes of a first type to a first region of a sample, wherein at least a subset of the probes of the first type includes a first spatial barcode, providing a plurality of probes of a second type to a second region of the sample, wherein at least a subset of the probes of the second type includes a second spatial barcode, and providing a probe of a third type to a third region of the sample, wherein the probe of the third type comprises a third spatial barcode. | 2022-03-10 |
20220073975 | ISOTHERMAL AMPLIFICATION WITH ELECTRICAL DETECTION - Some embodiments of the methods provided herein relate to amplifying and detecting a target nucleic acid. Some such embodiments include performing a recombinase polymerase amplification (RPA) and, optionally, a second isothermal amplification reaction. In some embodiments, the second isothermal amplification reaction includes loop-mediated isothermal amplification (LAMP). In some embodiments, the second isothermal amplification reaction is performed in conjunction with the RPA. In some embodiments, the second isothermal amplification reaction is performed on amplification products of the RPA. Some embodiments also include detecting the presence of amplification products by measuring a modulation of an electoral signal such as impedance. | 2022-03-10 |
20220073976 | CYCLONE ROLLING CIRCLE AMPLIFICATION, DOUBLE PRIMER FOR ROLONY AND REPLI-ROLONY AND PAIR-END SEQUENCING PROCESS - Cyclone Rolling Circle Amplification (CRCA) based next-generation sequencing (NGS) using a multiple primer rolling circle amplification (RCA) reaction is generated using two or more tandem primers from the same strand on different locations of library adaptor regions of double stranded enriched targeted polymerase chain reaction (PCR) library products. This process allows multiple initiation and syntheses of the RCA reaction by an enzyme on the same circular template molecule, which is beneficial since the two or more primers complement each other in generating uniform amplification of the target circle population. Also, a method for keeping DNA nanoballs (also known as rolonies) compact, or more compact, and for pre-priming rolonies before sequencing to eliminate the hybridization of a seqeuncing primer after seeding the rolonies, and a Rolonies rolling circle amplification (RCA) based next-generation sequencing (NGS) using a dual Rolonies primer approach named REPLI-Rolony. | 2022-03-10 |
20220073977 | METHODS AND MATERIALS FOR ASSESSING NUCLEIC ACIDS - Provided herein are systems, kits, compositions and methods for sequencing library preparation and sequencing workflow (e.g., for the identification of mutations). In certain embodiments, provides herein systems and methods to identically barcode both strands of templates, and PCR-based enrichment of each strand that does not require hybridization capture. | 2022-03-10 |
20220073978 | METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING - The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR. | 2022-03-10 |
20220073979 | METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING - The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a mixed sample of DNA comprising DNA from both the mother of the fetus and from the fetus, and optionally from genotypic data from the mother and father. The ploidy state is determined by using a joint distribution model to create a plurality of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. The mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias, for example using massively multiplexed targeted PCR. | 2022-03-10 |
20220073980 | SEQUENCING BY COALESCENCE - A method of sequencing a single, elongated target polynucleotide molecule can include the steps of seeding a plurality of separately resolvable origins of polynucleotide synthesis along the single, elongated target polynucleotide; contacting the target polynucleotide with a polymerase and labelled nucleotides; incorporating a labelled nucleotide, using the polymerase, into a plurality of sequence fragments complementary to the target polynucleotide and originating from the origins of polynucleotide synthesis; identifying and storing the identity and positions of the labelled nucleotide incorporated into each of the plurality of sequence fragments; and repeating the incorporating and identifying steps until adjacent sequence fragments coalesce and result in continuous sequence reads spanning two or more adjacent sequence fragments. | 2022-03-10 |
20220073981 | INFLAMMATORY BIOMARKER SPECIFIC TO EXPOSURE TO 2-BUTANONE AND IDENTIFICATION METHOD USING SAME - The present invention provides a biomarker for identifying the expression of an inflammatory-response-associated gene that specifically causes a change in expression due to exposure to 2-butanone, which is harmful and found in indoor environments, and an identification method using the same, and particularly an inflammatory-response-associated gene, the expression of which is increased or decreased by exposure to 2-butanone in a human bronchial epithelial cell line model (BEAS-2B), and a method of identifying exposure and predicting an inflammatory response using the same. The biomarker of the present invention includes specific genes selected through RNA sequencing, and thus can be useful in monitoring and determining the exposure to 2-butanone in the environment, and can be utilized as a tool predicting the mechanism of inflammatory response and toxicity caused by exposure to 2-butanone. | 2022-03-10 |