| 10th week of 2012 patent applcation highlights part 36 |
| Patent application number | Title | Published |
|---|
| 20120058440 | Oven for Semiconductor Wafer - An oven is described that can more evenly heat the semiconductor wafer, even though the wafer may warp during heating. The oven may provide relatively uniform heating even though the type and location of warping may be unpredictable for any given wafer. The oven may have a heating surface divided into a plurality of heating zones that may each independently provide a given amount of heat to the wafer. The amount of heat provided by each zone may be determined using signals from sensors that sense the warping of the wafer. | 2012-03-08 |
| 20120058441 | ARRANGEMENT AND METHOD FOR HEATING OF A MAGNETIC MATERIAL - The present invention relates to an arrangement ( | 2012-03-08 |
| 20120058442 | SELF-LIGATING ORTHODONTIC BRACKET AND METHOD OF MAKING SAME - An orthodontic bracket for coupling an archwire with a tooth comprises a bracket body configured to be mounted to the tooth and a clip. A clip slot extends through the bracket body transversely to the archwire slot. Upon deflection of the clip, it contacts the bracket body at locations that were not in contact before the deflection. The clip may also have various forms and include various structures that cooperate with the body when the clip is deflected to restrict or stop the deflection. The orthodontic bracket may comprise a securing mechanism that is configured to secure the resilient ligating clip in the closed position. A method of manufacturing a shaped unsintered body for use in manufacturing an orthodontic bracket is provided. A portion of the sinterable particles may be removed using a stream of energy to yield a plurality of posts which are then deformed. | 2012-03-08 |
| 20120058443 | ORTHODONTIC DEVICE AND METHOD FOR MOUNTING AND REMOVING ORTHODONTIC CAP - In an orthodontic device | 2012-03-08 |
| 20120058444 | Variable Extension Spring For Orthodontics - A variable extension spring with two anchoring ends. Each anchoring end connected to an anchoring mechanism having engagement mechanisms. Engagement mechanisms may be eyelets. Engagement of the engagement mechanisms to intraoral features at different engagement positions allows variability in the extension length of the spring. Such variability allows the user to adjust the force exerted by the spring on the intraoral features. As intraoral features move overtime due to the force exerted by the spring, the extension length of the spring may change. Adjustment of the spring extension length is accomplished by engaging a different engagement mechanism to provide an engagement position that will extend the spring the desired length. The user is able to reduce inventory by being able to use the same spring several times to provide a desired range of forces for adjusting orthodontia. | 2012-03-08 |
| 20120058445 | DENTAL INSTRUMENT AND METHOD OF USE - A dental instrument for use in removing unremineralizable carious dentin from a tooth while generally maintaining remineralizable carious dentin. The instrument comprises an instrument body and an instrument head having scraping elements made of plastic. Also, a method of removing unremineralizable carious dentine from a tooth while generally maintaining remineralizable carious dentin, wherein the method comprises the use of the dental instrument. | 2012-03-08 |
| 20120058446 | CHUCKING DEVICE FOR A MEDICAL OR DENTAL HANDPIECE - A medical or dental handpiece comprising a tool holding/releasing device with a hollow shaft that can be put into a drive motion for holding a treatment tool, wherein the hollow shaft extends along a central axis and has a tool receptacle opening, a first holding unit and a second holding unit separate from the first holding unit and axially offset from the first holding unit relative to the central axis, wherein both holding units are designed to axially secure a treatment tool held in the hollow shaft and to transmit a torque to the treatment tool, and wherein both holding units have at least one shaped element each and a bore penetrating the outer wall of the hollow shaft, in which the at least one shaped element can be held in such a way that it projects through the bore into the interior of the hollow shaft, a locking sleeve operatively connected to the shaped elements that can move relative to the hollow shaft and that surrounds the hollow shaft and an operating element operatively connected to the locking sleeve for the movement or sliding of the locking sleeve. | 2012-03-08 |
| 20120058447 | INTERDENTAL WEDGE - The invention relates to an interdental wedge for use with a matrix band when preparing a filling on a tooth to be restored and/or for use with a rubber dam, by inserting the interdental wedge into an area near the gingiva between the tooth to be restored or to be treated and an adjacent tooth, with an insertion area having a cross section delimited by a lower face, by an inner face and by an outer face, wherein, in the state inserted near the gingiva, the lower face is directed towards the gum, the inner face is directed towards the tooth to be restored or to be treated, and the outer face is directed towards the adjacent tooth, wherein the inner face and the lower face run together in an edge, which is designed such that, in the state inserted near the gingiva, it bears on the tooth to be restored or to be treated, in order to provide a sealing action against passage of material. | 2012-03-08 |
| 20120058448 | PROSTHESIS MOUNTING DEVICE AND CARRIER TOOL FOR USE IN MINI IMPLANT FIXED/REMOVABLE PROSTHODONTIC APPLICATIONS - A prosthesis mounting device ( | 2012-03-08 |
| 20120058449 | Machined overdenture bar - Overdenture bars secured to a portion of a jaw bone provide a mounting platform for dental prosthetics. One method to form an overdenture bar includes obtaining a digital image of a portion of a mouth, transferring the image to a computer and forming a three dimensional image of the portion. A virtual overdenture bar is designed to terminate at opposing first and second distal ends and have a first thickness defined by opposing first and second surfaces. The overdenture bar has an extension projecting from at least one distal end adjacent the second surface. Each extension has a second thickness that is less than the first thickness. A socket extends through each extension for engagement with the dental prosthetic. A digital file of the virtual overdenture bar is transferred to a number controlled machine and the overdenture bar and extensions machined from a single piece of stock material. | 2012-03-08 |
| 20120058450 | OSTEOINTEGRATIVE INTERFACE - Dental implant with biomimetic osteointegrative interface has a substrate of biocompatible material with a first layer thereon, having a first concentration of oxide of the biocompatible material, enriched with a second concentration of phosphorus and with a third concentration of calcium. The ratio between the concentration of calcium and the concentration of phosphorus is greater than two and the biomimetic osteointegrative interface further comprising a second layer having a first surface in contact with the first layer, and a second surface, the second layer having a fourth concentration of oxide enriched with a fifth concentration of calcium and a sixth concentration of phosphorus, the fourth concentration being less than the fifth and sixth concentrations and the ratio between the fifth concentration of calcium and the sixth concentration of phosphorus being no less than three. | 2012-03-08 |
| 20120058451 | DENTAL SECURING MECHANISM WITH TAPER SEAL - A method comprises attaching an abutment to an implant body so as to form a seal and cover the implant body, the implant body having a top portion with an external tapered surface and a bottom portion secured within a jaw bone of a patient. The abutment has a top portion adapted to secure a prosthetic component and a bottom portion adapted to be positioned below a gumline of the patient. The bottom portion of the abutment is shaped to attach to and fit over the implant body. The bottom portion of the abutment has a cavity with an internal tapered surface. The internal tapered surface of the abutment and the external tapered surface of the implant body form a Morse taper. | 2012-03-08 |
| 20120058452 | COMFORT HEAD MINI DENTAL IMPLANT - A one piece screw type mini dental implant ( | 2012-03-08 |
| 20120058453 | HOT MELT DENTAL MATERIALS AND DEVICES AND METHODS FOR USING THE SAME - Example embodiments of the present invention include hot melt dental adhesive materials that can create a reversible bond. In addition, example embodiments of the present invention include hot melt dental impression materials that can be used to accurately and efficiently obtain a dental impression. Moreover, example embodiments of the invention include devices and methods for use with the hot melt dental adhesive materials and the hot melt dental impression materials disclosed herein. The hot melt dental adhesive materials and the hot melt dental impression materials can provide dental professionals with dental materials that are more effective, efficient, and easier to use when compared to conventional dental adhesive materials and conventional dental impression materials. | 2012-03-08 |
| 20120058454 | Sketching Devices having Improved Geometry - An improved sketching device is disclosed having a substantially flat base and a regular grid of at least 20×20 bumps disposed on the base. A bottom of the each of the bumps can be spaced apart from a bottom of an adjacent bump by a first distance comprising between 0.005-5 mm. The raised bumps can be sized and positioned to form a plurality of intersecting grooves. | 2012-03-08 |
| 20120058455 | Device and Method for Facilitating Conversation - A system and method for facilitating conversation. In particular, the system discloses a handheld device suitable for use in engaging conversation. The device includes a data store for storing a plurality of conversation starters. The plurality of conversation starters is associated with a theme. The device includes a selector that is associated with a subset of the plurality of conversation starters. The subset defines a category of conversation starters consistent with the theme. The device includes a processor configured to receive and acknowledge activation of the selector. Upon activation, the processor selects a conversation starter of the subset of the plurality of conversation starters, as a selected conversation starter. A communicator, such as a speaker, is included for presenting the selected conversation starter. | 2012-03-08 |
| 20120058456 | Casting Training Aid Including A Target Board That Depicts An Aquatic Scene - A casting training aid and method of use is disclosed, including a casting target board having a plurality of target holes, with a front side depicting an aquatic scene. The aquatic scene can depict an above-water scene, an underwater scene, and/or a water body surface. The casting training aid can also include at least one obstruction mounted on the target board and/or near target holes. This aid allows a user having a rod, line, and lure or simulated lure to practice casting at the casting training aid in order to improve the user's skill of placing the lure near the fishing target. Scores can be given for engaging a target hole with a lure, with more difficult targets yielding more points. This training aid can be enjoyed by all ages and skill levels indoors or out, in a pool, lake or where ever, while improving an individual's casting skills. | 2012-03-08 |
| 20120058457 | Multimodal Ultrasound Training System - The present invention teaches a medical procedure training system based on a PC platform that provides multimodal education within a virtual environment. The system integrates digital video, three-dimensional modeling, and force-feedback devices for the purpose of training medical professionals medical procedures. | 2012-03-08 |
| 20120058458 | INTERACTIVE METHOD AND SYSTEM FOR TEACHING DECISION MAKING - The present invention is directed to an interactive computer tool for presenting and presenting documents directed to decision-making. The computer tool requires users to identify choice options and, for each choice option, relevant consequences, outcomes, and/or goals, for evaluating outcomes therefor. | 2012-03-08 |
| 20120058459 | Democratic Process of Testing for Cognitively Demanding Skills and Experiences - A system and methods for grading a candidate based on evaluations by assessors. The assessors evaluate questions that the candidate authors, and answers that the candidate prepares. Each assessor provides a question score, or answer score, as an objective measure of the evaluation. The methods retrieve a grade for each assessor, and calculate a grade for the candidate based on the question score, or answer score, and the grade for each assessor. The methods grade each assessor based on evaluations by other assessors. The other assessors evaluate the question score, or answer score, and provide an evaluation score as an objective measure of the evaluation. The methods retrieve a grade for each other assessor, and calculate a grade for the assessor based on the evaluation score, and the grade for each other assessor. | 2012-03-08 |
| 20120058460 | SLUG CONTROL DURING THERMAL CYCLING - The present invention, in one aspect, provides methods and systems for controlling slugs using temperature dependent fluorescent dyes. In some embodiments, the present invention uses one or more techniques to enhance the visibility of slugs, enhance a system's ability to differentiate between slugs, and enhance a system's ability to identify the positions of slugs. | 2012-03-08 |
| 20120058461 | MOLECULAR DETECTION OF XMRV INFECTION - The present invention relates generally to assays for the detection of Xenotropic Murine Leukemia Virus-related Retrovirus (“XMRV”) and diseases associated with XMRV infection. In particular, the invention relates to XMRV-related nucleic acids having significant diagnostic and screening utilities and methods of using the same. | 2012-03-08 |
| 20120058462 | MOLECULAR DETECTION OF XMRV INFECTION - The present invention relates generally to assays for the detection of Xenotropic Murine Leukemia Virus-related Retrovirus (“XMRV”) and diseases associated with XMRV infection. In particular, the invention relates to XMRV-related nucleic acids having significant diagnostic and screening utilities and methods of using the same. | 2012-03-08 |
| 20120058463 | HYDROPHOBIC, FUNCTIONALIZED PARTICLES - The present invention relates to a stable mixture comprising surface-modified particles which are obtained by reacting metal oxide or semimetal oxide particles with at least one compound selected from among silicon-comprising compounds bearing one, two or three alkoxy radicals and at least one solvent, at least one surface-active substance or a mixture thereof, the use of these particles in systems in which they are brought into contact with at least one solvent, where the mass ratio of solvent to modified particle is greater than 500, and also the use of these particles in agglomeration-deagglomeration cycles. | 2012-03-08 |
| 20120058464 | Assay Methods Using Array of Test Zones - A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte. Capillary structures of the devices or used in the methods may comprise a matrix and the devices may comprise control elements and methods for assaying of sample may use corresponding controlling activities. | 2012-03-08 |
| 20120058465 | METHOD AND DEVICE FOR ASSAY - A device for assay can evenly develop solution, and performs highly accurate and sensitive measurement. A first device part ( | 2012-03-08 |
| 20120058466 | DIFFERENTIAL IMMUNOASSAY FOR PRRS VACCINE ANTIBODY - The present invention relates to immunoassays for serologically differentiating animals naturally infected with PRRS virus from animals vaccinated against PRRS. The immunoassays provide detection of at least a portion of the N terminal region of the 2b portion of PRRSV. The immunoassay is preferably an enzyme-linked immunosorbent assay (ELISA). | 2012-03-08 |
| 20120058467 | APPARATUS AND METHOD FOR DETECTING DNA DAMAGE - The invention relates to an apparatus and an in-situ method for detecting cellular DNA damage. The invention is particularly suited for use in Comet assays and for automation of such assays. The apparatus comprises a multiwell plate, the plate comprising an array of wells held in fixed relationship with each other and each well having an axis, side walls, and a base and wherein the well is provided with electrode pairs disposed therein; and wherein there is provided means for parallel connection of the electrode pairs to an external voltage supply. | 2012-03-08 |
| 20120058468 | ADAPTORS FOR NUCLEIC ACID CONSTRUCTS IN TRANSMEMBRANE SEQUENCING - The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids. | 2012-03-08 |
| 20120058469 | FUNCTIONALIZED CYANINE DYES (PEG) - The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates. | 2012-03-08 |
| 20120058470 | ELECTRICAL WIRING OF POLYNUCLEOTIDES FOR NANOELECTRONIC APPLICATIONS - The present invention relates to incorporation and patterning of polynucleotide molecular wires onto surfaces. In one embodiment, two or more thiol-modified polynucleotide anchors are separately attached to metal contacts that are in turn separately attached to a substrate. Each polynucleotide anchor contains an unpaired region of bases that when bound to complimentary regions of a polynucleotide bridge molecule allow for electrical communication between contacts, and therefore detection of the polynucleotide bridge. | 2012-03-08 |
| 20120058471 | IDENTIFICATION OF NUCLEIC ACID SEQUENCES - The invention provides a method for use in the detection of a target nucleic acid comprising the steps of: (i) contacting a single-stranded probe nucleic acid with a sample of interest under conditions effective to generate a probe/target nucleic acid duplex by specific hybridisation of said probe nucleic acid to a target nucleic acid, if said target nucleic acid is present; (ii) contacting any probe/target nucleic acid duplex with an exonuclease to effect digestion of the duplex and release of a label molecule from the duplex; and (iii) detecting the label by Raman spectroscopy. | 2012-03-08 |
| 20120058472 | METHOD AND SYSTEM FOR NUCLEIC ACID DETECTION USING ELECTROCONDUCTIVE OR ELECTROCHEMICALLY ACTIVE LABELS - A method for electrochemically or electrically detecting nucleic acids, utilizes electrochemically active or electrically conductive reporter materials. An electric voltage is applied and electric signals are measured to the electrodes that are suitable for detecting or quantifying the nucleic acid(s) in a sample. This technique is suitable for point-of-use applications, e.g. detecting bioanalytes in remote locations. A microchip, device, kit used adapted to be used for this method is also disclosed. | 2012-03-08 |
| 20120058473 | Molecular Adaptors for Dye Conjugates - In various embodiments, the present invention provides fluorescent dyes that are linked to another species through an adaptor moiety. In an exemplary embodiment, the dye is linked to a polyphosphate nucleic acid through an adaptor. An adaptor can be a component of a linker. These conjugates find use in single molecule DNA sequencing and other applications. In various embodiments, the dye moiety is a cyanine dye. Cyanine dyes that are highly charged, such as those including multiple sulfonate, alkylsulfonate, carboxylate and/or alkylcarboxylate moieties are examples of cyanine dyes of use in the compounds of the invention. | 2012-03-08 |
| 20120058474 | PROBE-ANTIPROBE COMPOSITIONS AND METHODS FOR DNA OR RNA DETECTION - The invention provides novel compositions and methods for detecting unlabeled nucleic acid targets using labeled polynucleotide probes and partially complementary antiprobes. The interaction of probes, antiprobes and targets result in signaling changes that indicate target frequency. This novel detection mechanism is called a DNA detection switch, and it enable end-point detection, microarray detection and real-time PCR detection of a variety of nucleic acid targets including microbial species and subspecies, drug resistant mutants, and pathogenic strains. | 2012-03-08 |
| 20120058475 | Detection of Chromosomal Inversions - A method and a kit for the identification of chromosomal inversions is described. Chromosomal inversions are difficult to detect unless they are quite large. The improved ability to detect chromosomal inversions is important to a number of medical applications, such as cancer and birth defects, as examples. Reporter species are attached to oligonucleotide strands designed such that they may hybridize to portions of only one of a pair of single-stranded sister chromatids prepared by the CO-FISH procedure, as an example. If an inversion has occurred, these marker probes will be detected on the sister chromatid at the same location as the inversion on the first chromatid. | 2012-03-08 |
| 20120058476 | Method Of Oligonucleotide Labeling Using Cycloaddition Reaction - The invention provides a novel method of labeling oligonucleotides, with reporter moieties, including but not limited to, quenchers, fluorophores, biotin, digoxigenin, peptides and proteins. In addition, this invention provides a method of detecting hybridization of oligonucleotides. This invention also provides novel azo quenchers having the general formula shown below. The invention further provides compositions comprising labeled oligonucleotides and solid supports. The invention also provides kits comprising at least one composition of the present invention. | 2012-03-08 |
| 20120058477 | METHOD FOR DETECTING THE EXPRESSION OF CYCLIN B2 GENE BY REAL-TIME QUANTITATIVE PCR - A method and a kit for quantitative detection of the expression of cyclin B2 gene in blood samples, especially in serum. | 2012-03-08 |
| 20120058478 | MULTIMERS OF S. SOLFATARICUS SINGLE-STRANDED DNA-BINDING PROTEIN AND METHODS OF USE THEREOF - The invention provides multimers of | 2012-03-08 |
| 20120058479 | WORKFLOW TIMING BETWEEN MODULES - The invention relates to a method of isolating and analyzing an analyte using an analytical apparatus which comprises modules of different types, wherein any one module of one type has a specific, pre-defined timing for carrying out its workflow. | 2012-03-08 |
| 20120058480 | ANTIGENIC APPROACH TO THE DETECTION AND ISOLATION OF MICROPARTICLES ASSOCIATED WITH FETAL DNA - The present disclosure provides methods of quantifying and isolating microparticles of fetal from maternal bodily fluids such as cell free maternal plasma. Antibodies or combinations of antibodies that selectively bind fetal microparticles in maternal bodily fluid permit quantification by flow cytometry and isolation through flow cytometry based sorting and immunopurification. | 2012-03-08 |
| 20120058481 | Quantitative Real Time PCR Assay Using FRET Dual-Labeled Primers - This specification generally relates to non-radioactive methods of non-radioactive real-time PCR using FRET dual-labeled primers. | 2012-03-08 |
| 20120058482 | FUNCTIONALIZED CYANINE DYES (PEG) - The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates. | 2012-03-08 |
| 20120058483 | MITOCHONDRIAL MUTATIONS AND REARRANGEMENTS AS A DIAGNOSTIC TOOL FOR THE DETECTION OF SUN EXPOSURE, PROSTATE CANCER AND OTHER CANCERS - Mitochondrial DNA deletions useful for the detection of cancers and sun exposure are provided. In particular, methods and kits for detecting mitochondrial DNA deletions for the early detection, diagnosis and progression of prostate cancer, sun exposure and non-melonoma skin cancer are provided. | 2012-03-08 |
| 20120058484 | METHODS AND MATERIALS FOR ASSESSING A MAMMAL'S SUSCEPTIBILITY FOR VENOUS THROMBOEMBOLISM - This document provides methods and materials involved in assessing a mammal (e.g., a human) for a susceptibility to develop venous thromboembolism. For example, methods and materials for using a genetic variation in an ABO nucleic acid (e.g., rs | 2012-03-08 |
| 20120058485 | RAPID AND COMPREHENSIVE IDENTIFICATION OF PROKARYOTIC ORGANISMS (CONTINUATION) - An improved method for rapid identification of microorganisms is disclosed, along with sequences of PCR primers optimized for this purpose. The primers are designed based on information analysis of sequences from a large number of organism to amplify certain segments of genomic DNA whose sequences are unique among different organisms. The PCR products are compared with a DNA sequence database to obtain the identity of the microorganisms. This approach provides an accurate and fast identification and taxonomic assignment of microbial species. | 2012-03-08 |
| 20120058486 | GENETIC RISK ASSESSMENT TECHNOLOGY FOR EPITHELIAL CANCER INVOLVING GENE-ENVIRONMENT INTERACTION BETWEEN ERCC5 AND TOBACCO USE - Methods and compositions for assessing ERCC5 gene expression in view of certain environmental exposures and determining the risk of an individual for developing one or more epithelial cancers are provided. | 2012-03-08 |
| 20120058487 | COMPOSITIONS AND METHODS FOR DETECTING KLEBSIELLA PNEUMONIAE - Four highly conserved genes, encoding translation elongation factor Tu, translation elongation factor G, the catalytic subunit of proton-translocating ATPase and the RecA recombinase, are used to generate species-specific, genus-specific, family-specific, group-specific and universal nucleic acid probes and amplification primers to rapidly detect and identify algal, archaeal, bacterial, fungal and parasitical pathogens from clinical specimens for diagnosis. The detection of associated antimicrobial agents resistance and toxin genes are also under the scope of the present invention. | 2012-03-08 |
| 20120058488 | WIRELESS BASED MARINE PATHOGENS DETECTION SYSTEM - Embodiments of the present Invention provide antibody functionalized high electron mobility transistor (HEMT) devices for marine or freshwater pathogen sensing. In one embodiment, the marine pathogen can be | 2012-03-08 |
| 20120058489 | USE OF MEGALIN IN URINE AS MARKER FOR DETECTING RENAL DISORDER - This invention provides a simple means for detecting a renal disorder, a diagnostic marker for a renal disorder that enables prognostic prediction of a renal disorder (e.g., diabetic nephropathy and IgA nephropathy) and evaluation of the degree of nephropathy at the phase of stage-II diabetic nephropathy by measuring the megalin level in urine associated with a renal disorder used for the detection means, and use of such marker. The invention also provides the use of human megalin obtained from the urine sample of a subject as a marker for detecting a renal disorder. | 2012-03-08 |
| 20120058490 | METHOD FOR DETECTING AN ANTIGEN - Provided is a method for detecting an antigen without use of a labeled-antibody. A support having an antibody and a multi-copper oxidase CueO immobilized thereon is brought into contact with a first buffer solution containing the antigen, a current is measured by a potentiostat method using the support and a second buffer solution, and when the measured current is greater than or equal to 1.5×(blank value), it is determined that the antigen exists. The second buffer solution contains a substrate of the CueO and has an ionic strength falling within a range of not less than 0.3 mM and not more than 1.0 mM. | 2012-03-08 |
| 20120058491 | METHOD FOR INDUCTION OF DIFFERENTIATION OF ES CELL - It is an object of the present invention to establish a system for reliably differentiating an ES cell into a hepatic cell. The present invention provides a method for inducing the differentiation of an ES cell into a hepatic cell, which comprises, in the presence of an M15 cell, culturing a mammal-derived ES cell in the presence of activin and bFGF, and then culturing the ES cell in the presence of dexamethasone, HGF, and oncostatin M. | 2012-03-08 |
| 20120058492 | Method and a Kit To Detect Malignant Tumors and Provide a Prognosis - A method and kit is provided to quantifying and qualifying exosomes in human cell derived samples or in body fluid based on expression of TM9-superfamily proteins on the exosomes. Furthermore, a method and a kit to diagnose malignant tumors is provided. The disclosure also provides a method to monitor tumor growth. | 2012-03-08 |
| 20120058493 | BIOMARKER FOR MONITORING PATIENTS - The present invention is in the field of immunotherapy and relates to methods for determining the efficacy of certain immunotherapy treatments. The methods of the invention include measuring special biomarker at some time following the initiation of immunotherapy treatment to evaluate the clinical outcome of the said treatment. The invention thus has applications to the field of medicine. | 2012-03-08 |
| 20120058494 | Quenched Dendrimeric Dyes for Florescence Detection - The present invention presents designs for high extinction quenched “dyedrons” that can be activated by conversion of a single acceptor/quencher in the molecular assembly to a fluorescent state. The quencher is activated by noncovalent binding to a unique complementary expressible fluorogen activating peptide (FAP). In this way, the quencher serves as the homogeneous switch, receiving energy efficiently from each of the donor molecules of the dendronic antenna, and releasing it as fluorescence only when activated by binding. The sum of the extinction of the multiple dyes on the antenna will provide dramatic enhancements in the effective brightness of the probe in standard imaging systems. This approach provides a set of probes with exceptional brightness, specifically targeted to an expressed tag that activates the fluorescence of the dyedron. | 2012-03-08 |
| 20120058495 | Recombinant fragments and synthetic peptides of 17-kDa polypeptide useful in detecting Bartonella henselae - The present disclosure describes recombinant and synthetic polypeptides of | 2012-03-08 |
| 20120058496 | PHYSIOLOGICALLY ACTIVE SUBSTANCE COLLECTING DEVICE AND BIOLOGICAL INFORMATION ACQUISITION METHOD - A physiologically active substance collecting device, includes: a collecting section brought into contact with a body surface of a living organism to acquire a physiologically active substance from the body surface; and a liquid sending means for sending a solvent to the collecting section, the collecting section having an aperture at which the solvent flown by being sent from the liquid sending means contacts the body surface. | 2012-03-08 |
| 20120058497 | DIAGNOSTIC REAGENT FOR CROHN'S DISEASE - The present invention provides a reagent and a method of safely, conveniently, and specifically diagnosing Crohn's disease. Provided are a diagnostic method for Crohn's disease in a subject, including measuring antibodies against one kind or more of dietary components selected from the group consisting of grapefruit, alfalfa, avocado, cabbage, green pepper, lettuce, onion, potato (white), spinach, tomato, oat, pecan, yeast, cane sugar, celery, buckwheat, corn, rice and soy, and a diagnostic reagent or kit for Crohn's disease, containing the above-mentioned preparation of a dietary component. | 2012-03-08 |
| 20120058498 | Compositions and Methods for Characterizing Arthritic Conditions - The present invention relates to autoantibodies against 14-3-3 proteins or circulating immune complexes thereof and the detection of such for the diagnosis and prognosis of an arthritic condition. | 2012-03-08 |
| 20120058499 | Bioluminescent Detection of Protease Activity - Methods for bioluminescent detection of the activity of proteolytic enzymes including ubiquitin (Ub) and ubiquitin-like (Ubl) proteolytic enzymes are disclosed. | 2012-03-08 |
| 20120058500 | PLATELET AGGREGATION USING A MICROFLUIDICS DEVICE - A microfluidics device to provide real time monitoring of platelet aggregation of a biological sample obtained from a subject. The device comprises a channel configured for passage of the biological sample, the channel comprising a protrusion configured to induce an upstream region of shear acceleration coupled to a downstream region of shear deceleration and defining there-between a region of peak rate of shear, the downstream region of shear deceleration defining a zone of platelet aggregation. The device further comprises a platelet detection means for detecting aggregation of platelets in the zone of aggregation as a result of passage of the biological sample through the channel. Methods to assess real time platelet aggregation of a biological sample obtained from a subject are further described. | 2012-03-08 |
| 20120058501 | OXIDIZED GLUTATHIONE ASSAY - The present invention provides an assay for detection of oxidized glutathione (GSSG). | 2012-03-08 |
| 20120058502 | Method for Identifying Protease Inhibitors - The invention describes a method for assaying HCV NS3 protease activity using an NS3•4A protease molecule. The invention also provides a method for screening and identifying modulators of NS3 protease. | 2012-03-08 |
| 20120058503 | Novel Indicator Platform - A novel indicator platform comprises a plurality of 1H-lndol-3-yl indicator compounds that are capable of converting to a signalophore compound in response to an external stimulus. In one class of indicator compounds, the resulting signalophores are 2-benzylideneindoline compounds that are formed by an intermolecular Aldol-type process; in a further class of indicator compounds, the resulting signalophores are 10H-indolo[1,2-a]indole compounds that are formed by an intramolecular Aldol-type process. The indicators can be used in a wide array of applications relating, for example, to biological systems or optical data storage. | 2012-03-08 |
| 20120058504 | METHODS AND APPARATUS FOR DIELECTROPHORETIC SHUTTLING AND MEASUREMENT OF SINGLE CELLS OR OTHER PARTICLES IN MICROFLUIDIC CHIPS - The invention relates to a microfluidic device. The microfluidic device comprises a fluid chamber comprising a particle retention region for retaining at least one particle, such as a cell. The microfluidic device also comprises a plurality of electrodes extending into the particle retention region for applying a dielectrophoretic (DEP) force to controllably move the particle within the particle retention region. The invention also relates to methods of using the microfluidic device to controllably move the particle within the microfluidic device and to monitor, observe, or measure a parameter of the particle. The particle movement may be caused by a DEP force and/or a fluidic force. | 2012-03-08 |
| 20120058505 | Cytosolic Delivery of Materials with Endosome-Disrupting Colloids - A facile procedure to deliver nanocrystals to the cytosol of live cells that is both rapid and general. The technique employs a unique cationic core-shell polymer colloid that directs nanocrystals to the cytosol of living cells within a few hours of incubation. The present methods and compositions enable a host of advanced applications arising from efficient cytosolic delivery of nanocrystal imaging probes: from single particle tracking experiments to monitoring protein-protein interactions in live cells for extended periods. | 2012-03-08 |
| 20120058506 | MICRONEEDLE - The application relates to microfluidic needles and their manufacturing methods. The microfluidic needle comprises an interface portion containing at least one fluid communication channel, an elongated needle portion ( | 2012-03-08 |
| 20120058507 | Clonal Derivation and Cell Culture quality Control Screening Methods - The invention provides methods of label-free detection of changes in cell populations and mixed cell populations. | 2012-03-08 |
| 20120058508 | DETECTOR FOR CHEMICAL COMPOUNDS - Systems and methods to analyze contaminants including a plurality of stages configured to detect contaminants in a sample, wherein the plurality of stages are configured to detect a plurality of contaminants at substantially the same time. | 2012-03-08 |
| 20120058509 | APPARATUS AND METHOD FOR AFFIXING FROZEN TISSUE SECTIONS TO GLASS OR MEMBRANE MICROSCOPE SLIDES - This invention is an apparatus and method for affixing frozen tissue sections to microscope slides. It has three elements; teasing implements, an affixing block and a heating element. The elements are used inside a cryostat prior to processing for molecular biology procedures such as immunohistochemistry or laser micro dissection. The tissue sections are collected onto an affixing block which has indentations that decrease the contact surface area while providing structural support. The tissue sections are then transferred onto a slide via transient application of a heating element to the back of slide. The invention decreases folding of tissue sections, increases morphological consistency, enables the placement of many sections on one slide and enables the adherence of tissue sections with greater thickness. | 2012-03-08 |
| 20120058510 | ENZYME COMPLEX FROM TRICHODERMA REESEI AND P. FUNICULOSUM ENZYMES - The invention relates to an improved enzyme complex having a plurality of enzyme activities of an expression product obtained by fermentation of the genus | 2012-03-08 |
| 20120058511 | MUTANT MICROORGANISM AND METHOD FOR PRODUCING PEPTIDE USING THE SAME - The present invention provides a method for producing the peptides comprising:
| 2012-03-08 |
| 20120058512 | METHOD OF EXPRESSING FOREIGN PROTEIN IN PLASTIDS - The present invention relates to a method of expressing a foreign protein in the plastid of a host cell and secreting said protein into the cytoplasm of the host cell comprising the steps of making a construct of vector linked to a coding sequence of the fusion protein comprising of signal peptide sequence followed by in-frame fusion to a foreign gene; and stably integrating said construct into the plastid genome. The present invention also relates to a method of targeting the expressed and secreted proteins from the plastids to the nucleus of the host cell. The present invention further relates to the method where the host cell is of any higher plant or any organism including single cell algae. | 2012-03-08 |
| 20120058513 | METHOD FOR PRODUCING HUMAN RECOMBINANT INSULIN - The invention relates to biotechnology and can be used for producing human recombinant insulin for preparing medicinal agents for the treatment of pancreatic diabetes. A variety of recombinant plasmid DNAs which contain an artificial gene and encode the human insulin precursor is proposed. The biosynthesis of a hybrid polypeptide is induced using isopropyl-thiogalactopyranoside so that the post-induction level of the hybrid polypeptide is equal to or greater than 25% of the total cellular protein. According to the claimed procedure, human insulin is produced by cultivating a producer strain containing one of the recombinant plasmids, isolating inclusion bodies, solubilizing and renaturing the fusion protein, and enzymatically degrading and chromatographically purifying said protein. The invention simplifies the process for producing human recombinant insulin and increases the yield thereof. | 2012-03-08 |
| 20120058514 | COLD ACTIVE ENZYME AND METHOD THEREOF - The objective of the present invention is to obtain a low temperature producing enzyme and more particularly, the enzyme provides a bifunctional purpose of varying its enzyme activity into activity of another enzyme. The present invention has overcome problems such as ability of obtaining an enzyme at low temperature. | 2012-03-08 |
| 20120058515 | METHOD OF REGULATING OLIGONUCLEOTIDE FUNCTIONALITY - The present invention relates generally to a method of regulating oligonucleotide functionality and, more particularly, to a method of regulating the functionality of a primer or probe. The method of the invention is designed to provide a means to selectively inactivate or activate the functionality of an oligonucleotide, such as a primer, thereby providing means to regulate the progress of any method using that oligonucleotide. The development of a means to regulate the functionality of an oligonucleotide, such as a primer, is useful in a range of applications including, but not limited to, amplification reactions such as PCR, isothermal amplification and nucleic acid strand extension. With respect to amplification reactions, these have wide utility including the diagnosis and/or monitoring of disease conditions which are characterised by specific gene sequences and the characterisation or analysis of specific gene regions of interest. | 2012-03-08 |
| 20120058516 | SEALING MULTIWELL PLATES - The present invention relates to a heat sealable member comprising a sheet of heat sealable material to a multiwell plate, wherein the sheet is mounted in a frame, and wherein said frame is sized to fit over a multiwell plate so that the sheet is contacted with at least one of the rims of the top of the plate and the individual wells; as well as to the use of the heat sealable member with automatic plate handling apparatus, especially in PCR processes. The present invention further relates to a method for sealing multiwell plates. | 2012-03-08 |
| 20120058517 | Recombinase Polymerase Amplification - This disclosure describes related novel methods for Recombinase-Polymerase Amplification (RPA) of a target DNA that exploit the properties of recombinase and related proteins, to invade double-stranded DNA with single stranded homologous DNA permitting sequence specific priming of DNA polymerase reactions. The disclosed methods have the advantage of not requiring thermocycling or thermophilic enzymes, thus offering easy and affordable implementation and portability relative to other amplification methods. Further disclosed are conditions to enable real-time monitoring of RPA reactions, methods to regulate RPA reactions using light and otherwise, methods to determine the nature of amplified species without a need for gel electrophoresis, methods to improve and optimize signal to noise ratios in RPA reactions, methods to optimize oligonucleotide primer function, methods to control carry-over contamination, and methods to employ sequence-specific third ‘specificity’ probes. Further described are novel properties and approaches for use of probes monitored by light in dynamic recombination environments. | 2012-03-08 |
| 20120058518 | NUCLEIC ACID ISOTHERMAL AMPLIFICATION METHOD - A nucleic acid isothermal amplification method includes: performing a reverse transcription reaction to reverse-transcribe a target RNA strand into a template DNA strand; irradiating a reaction solution with light to dissociate a photodegradable protecting group bound to a nucleotide in a sequence of an oligonucleotide primer; and performing an amplification reaction for the template DNA strand. | 2012-03-08 |
| 20120058519 | METHOD, DEVICES, AND SYSTEMS FOR FLUID MIXING AND CHIP INTERFACE - In one aspect, the present invention provides methods, devices, and systems for ensuring that multiple components of a mixture are fully mixed in a continuous flow microfluidic system while ensuring that mixing between segments flowing through the chip is minimized. In some embodiments, the present invention includes mixing fluids in a droplet maintained at the tip of a pipette before the mixture is introduced to the microfluidic device. In another aspect, the present invention provides methods, devices, and systems for creating segments that move through a microfluidic chip with minimal mixing between segments. The microfluidic chip may have an interface chip and a reaction chip. In some embodiments, the present invention includes creating segments that flow through an interface chip and a reaction chip, wherein the interface chip and a reaction chip have separate flow control mechanisms and produce minimal mixing between segments. | 2012-03-08 |
| 20120058520 | METHOD FOR SYNTHESIS OF DOUBLE-STRANDED DNA CORRESPONDING TO RNA, AND METHOD FOR AMPLIFICATION OF THE DNA - An object of the present invention is to provide an inexpensive and simple method for synthesis of a double-stranded DNA corresponding to a particular RNA, and a method for amplification of the aforementioned double-stranded DNA. The present invention relates to a method for synthesis of a double-stranded DNA having a nucleotide sequence corresponding to template RNA having polyA, comprising step 1 in which reverse transcription reaction of template RNA is carried out employing oligo(dT)primer to which DNA fragment having a known sequence has been added at the 5′-terminal, to obtain a single-stranded DNA, and step 2 in which double strand formation reaction of single-stranded DNA obtained in step 1 is carried out employing a random primer to which DNA fragment having a known sequence has been added at the 5′-terminal, in the presence of polymerase which does not have 3′→5′ exonuclease activity nor strand displacement activity, to obtain a double-stranded DNA, as well as a method for amplification of a double-stranded DNA having a nucleotide sequence corresponding to template RNA having polyA, further comprising step 3 in which PCR reaction is carried out using the obtained double-stranded DNA. | 2012-03-08 |
| 20120058521 | ENZYMATIC OLIGONUCLEOTIDE PRE-ADENYLATION - Methods and compositions for making and using pre-adenylated oligonucleotide sequences are provided. | 2012-03-08 |
| 20120058522 | ADENOVIRAL VA1 POL III EXPRESSION SYSTEM FOR RNAI EXPRESSION - An adenoviral VA1 Pol III expression system for RNAi expression is provided. | 2012-03-08 |
| 20120058523 | TEMPERING OF CELLULOSIC BIOMASS - The present invention is directed to improved systems and methods for reducing costs and increasing yields of cellulosic ethanol. In particular, the present invention provides plants genetically transformed for increased biomass, expression of lignocellulolytic enzyme polypeptides, and/or simplification of harvesting and downstream processing. Also provided are methods for processing biomass from these transgenic plants that involve less severe and/or less expensive pre-treatment protocols that are typically employed. Such methods allow, among other things, reduced costs associated with externally applied lignocellulolytic enzyme polypeptides. | 2012-03-08 |
| 20120058524 | METHODS FOR INCREASING THE YIELD OF FERMENTABLE SUGARS FROM PLANT STOVER - Methods for increasing yield of fermentable sugars from plant stover are provided. The methods include using plants homozygous for two brown midrib mutations, bm1 and bm3. The methods also include using plants homozygous for a mutation in a gene that results in reduced cinnamyl alcohol dehydrogenase activity, and a mutation in a gene that results in reduced 5-hydroxyconiferaldehyde/5-hydroxyconiferyl alcohol O-methyltransferase activity. The methods also include using transgenic plants that have reduced cinnamyl alcohol dehydrogenase activity and reduced 5-hydroxyconiferaldehyde/5-hydroxyconiferyl alcohol O-methyltransferase activity in comparison with wild-type plants. | 2012-03-08 |
| 20120058525 | METHOD FOR PRODUCING OIL BY YEAST - A method for producing oil by using yeast is provided. The method comprises utilizing at least one yeast strain serving as a working microorganism to produce the oil, wherein the yeast strain is genus | 2012-03-08 |
| 20120058526 | Methods and Systems for Processing Sugar Mixtures and Resultant Compositions - A method including: (a) selectively reacting a first sugar in a mixture which includes at least one second sugar to form a product mixture comprising a product of said first sugar; (b) separating said product of said first sugar from said product mixture; and (c) separating at least one of said at least one second sugar from said product mixture. | 2012-03-08 |
| 20120058527 | CAL A-RELATED ACYLTRANSFERASES AND METHODS OF USE, THEREOF - Compositions and methods relating to lipase/acyltransferase enzymes identified in prokaryotes and eukaryotes are described. These enzymes can be used in such applications as lipid stain removal from fabrics and hard surfaces and chemical synthesis reactions. | 2012-03-08 |
| 20120058528 | Homolactic Fermentation from Pentose - Provided is a lactic acid bacterium capable of homolactic fermentation using a pentose as a substrate, the lactic acid bacterium utilizing a pentose, and in which a phosphoketolase pathway is blocked and a pentose phosphate pathway is activated. Also provided is a method for producing lactic acid from a pentose using the lactic acid bacterium and a method for preparing the lactic acid bacterium. | 2012-03-08 |
| 20120058529 | METHOD FOR HIGHLY EFFICIENT PRODUCTION OF LACTIC ACID USING CANDIDA UTILIS - There is disclosed a yeast strain of | 2012-03-08 |
| 20120058530 | ENGINEERING THE PATHWAY FOR SUCCINATE PRODUCTION - This invention relates to the biocatalysts for the efficient production of succinic acid and/or other products from renewable biological feedstocks. The biocatalysts have a very high efficiency for the growth-coupled production of succinic acid and/or other products from carbohydrate feed stocks as a result of both genetic manipulations and metabolic evolution. More specifically, certain biocatalysts of the present invention produce succinic acid at high titers and yield in mineral salts media during simple pH-controlled, batch fermentation without the addition of any exogenous genetic material. The genetic manipulations of the present invention are concerned with the energy-conserving strategies coupled with the elimination of alternative routes for NADH oxidation other than the routes for succinic acid production. The biocatalysts contain glucose-repressed gluconeogenic phosphoenol pyruvate carboxykinase (pck) depressed by genetic modifications and a genetically-inactivated phosphotransferase system. In terms of succinic acid production efficiency, the biocatalysts of the present invention are functionally equivalent to succinate producing rumen bacteria such as | 2012-03-08 |
| 20120058531 | CONTINUOUS CULTURE FOR 1,3-PROPANEDIOL PRODUCTION USING HIGH GLYCERINE CONCENTRATION - The present invention concerns a new method for the production of 1,3-propanediol comprising culturing a microorganism on a culture medium with high glycerine content. The invention also concerns a new microorganism, or strain of microorganism, adapted for the production of 1,3-propanediol from medium comprising high glycerine content. | 2012-03-08 |
| 20120058532 | ENGINEERED MICROORGANISMS CAPABLE OF PRODUCING TARGET COMPOUNDS UNDER ANAEROBIC CONDITIONS - The present invention is generally provides recombinant microorganisms comprising engineered metabolic pathways capable of producing C3-C5 alcohols under aerobic and anaerobic conditions. The invention further provides ketol-acid reductoisomerase enzymes which have been mutated or modified to increase their NADH-dependent activity or to switch the cofactor preference from NADPH to NADH and are expressed in the modified microorganisms. In addition, the invention provides isobutyraldehyde dehydrogenase enzymes expressed in modified microorganisms. Also provided are methods of producing beneficial metabolites under aerobic and anaerobic conditions by contacting a suitable substrate with the modified microorganisms of the present invention. | 2012-03-08 |
| 20120058533 | RECOMBINANT BACTERIA AND THE USES THEREOF FOR PRODUCING ETHANOL - The present invention relates to recombinant bacteria and the uses thereof, particularly for the production of ethanol. The invention also relates to methods for the production of such bacteria, as well as to nucleic acid constructs suitable for such production. The invention specifically relates to bacteria lacking a functional LDH gene and/or containing a recombinant nucleic acid encoding a PDC and ADH. The bacteria of this invention may be produced from any stress-resistant bacteria. | 2012-03-08 |
| 20120058534 | OPTIMIZED BIOGAS (BIOMETHANE) PRODUCTION PROCESS - The present invention relates to a process for pretreatment of a feedstock in a pretreatment tank. Various parameters, such as oxidation-reduction potential, pH, and temperature, are monitored in the pretreatment tank to determine whether the oxidation-reduction potential, pH, and temperature are each within a predetermined range. The volume of feedstock inside the pretreatment tank is adjusted in response to a determination that one of the oxidation-reduction potential, pH, and temperature of the treated material are outside the corresponding predetermined ranges to maintain the oxidation-reduction potential, pH, and temperature of the treated material within operating conditions. | 2012-03-08 |
| 20120058535 | BIOFUEL PRODUCTION IN PROKARYOTES AND EUKARYOTES - Terpene synthases are enzymes that directly convert IPP & DMAPP to terpenes, such as fusicoccadiene. Described herein are methods and compositions for the production of terpenes and terpenoids for use as fuel molecules or other useful components. Genetically engineered enzymes capable of producing terpenes and terpenoids are also described. | 2012-03-08 |
| 20120058536 | POLYMER MADE OF A PRIMARY AMINE FUNCTIONALIZED POLYMER AND A HEMICELLULOSE - The present invention relates to a polymer made of a primary amine functionalized polymer and a hemicellulose e.g. chitosan and xyloglucan, wherein the primary amine functionalized polymer is covalently bound to the hemicellulose, and to a cross-linking agent composition comprising the polymer. A method wherein manufacturing a cellulose containing product comprises the steps of; providing a cellulose containing product; treating said cellulose product with a cellulose adsorbing agent comprising a polymer made of a primary amine functionalized polymer and a hemicellulose e.g. chitosan and xyloglucan, and optionally other additives is also provided. | 2012-03-08 |
| 20120058537 | CHIMERIC TRUNCATED AND MUTANT VARIANT OF TISSUE PLASMINOGEN ACTIVATOR (T-PA) RESISTANT TO PLASMINOGEN ACTIVATOR INHIBITOR-1 - The various embodiments herein provide a chimeric truncated and mutant variant of a tissue plasminogen activator (t-pa) and a method for preparing the same. According to an embodiment herein, the mutant variant comprises a signal sequence domain, followed by a chimeric tetrapeptide, followed by a tripeptide, followed by a kringle 2 domain, followed by a serine protease domain and a substituted amino acids at position 128-131. The substituted amino acids are AAAA (SEQ ID NO: 3) amino acids. The chimeric tetrapeptide is Gly-His-Arg-Pro (SEQ ID NO: 1). The chimeric tetrapeptide is at a position of 36 to 39 amino acid of the mutant variant. The tripeptide is Ser-Tyr-Glu. According to an embodiment herein, a chimeric truncated and mutant variant of a tissue plasminogen activator comprises a native t-pa deleted with Finger domain, a Growth Factor domain and a Kringle 1 domain, a chimeric tetrapeptide and a substituted amino acids at a position of 128-131. | 2012-03-08 |
| 20120058538 | Helper-free Rescue of Recombinant Negative Strand RNA Virus Systems - The present invention relates methods of generating infectious negative-strand virus in host cells by an entirely vector-based system without the aid of a helper virus. In particular, the present invention relates methods of generating infectious recombinant negative-strand RNA viruses intracellularly in the absence of helper virus from expression vectors comprising cDNAs encoding the viral proteins necessary to form ribonucleoprotein complexes (RNPs) and expression vectors comprising cDNA for genomic viral RNA(s) (vRNAs) or the corresponding cRNA(s). The present invention also relates to methods of generating infectious recombinant negative-strand RNA viruses which have mutations in viral genes and/or which express, package and/or present peptides or polypeptides encoded by heterologous nucleic acid sequences. The present invention further relates the use of the recombinant negative-strand RNA viruses or chimeric negative-strand RNA viruses of the invention in vaccine formulations and pharmaceutical compositions. | 2012-03-08 |
| 20120058539 | METHOD FOR ORTHOPOXVIRUS PRODUCTION AND PURIFICATION - The present invention relates to a method for producing and purifying a wild type, an attenuated and/or a recombinant Orthopoxvirus. The present invention relates to a purified wild type, attenuated and/or recombinant Orthopoxvirus obtained by the method of the invention and to a pharmaceutical composition, preferably a vaccine, comprising said purified Orthopoxvirus for the treatment and/or the prevention a cancer, an infectious disease and/or an autoimmune disorder, and uses thereof. The present invention also relates to the use of an immortalized avian cell line obtained from an avian cell belonging to the Anatidae family, in particular | 2012-03-08 |
