| 10th week of 2013 patent applcation highlights part 39 |
| Patent application number | Title | Published |
|---|
| 20130059283 | DEMENTIA CARE SUPPORT SYSTEM - A doctor is supported so that the doctor is able to present appropriate countermeasures corresponding to a symptom of a patient to the patient and a caregiver. A storage portion stores information of a question to be given to a patient of dementia or a caregiver of the patient; a countermeasure information selection portion selects any of the countermeasure information that is stored in the storage portion based on information of an answer of the patient or the caregiver to the question; and a countermeasure information output portion outputs the countermeasure information that is selected by the countermeasure information selection portion. | 2013-03-07 |
| 20130059284 | INTERACTIVE ELECTRONIC TOY AND LEARNING DEVICE SYSTEM - A device and system of information exchange for an educational toy is disclosed. Inside an external shell is placed a small computer. The Device interacts through auditory and visual responses, and accepts input through buttons, RFID accessories, smart cards and speech. The Device communicates with other like devices through a wireless Bluetooth connection. The Device receives information updates via a Bluetooth dongle that plugs into the end user's computer. When the Device is synchronized with the end user's computer, new information is pushed to (and stored in) the Device's internal computer. The end user's computer automatically downloads updates and configuration information from a central web server, via a background software application, which can be transferred during synchronization. Updates and configuration information are available via an online store hosted on the central server. The Device leverages the end user's smartphone to partially replace functionality performed by the internal computer. | 2013-03-07 |
| 20130059285 | AQUEOUS SOLUTION FOR THE PRESERVATION OF TISSUES AND ORGANS - It is provided an improved aqueous solution for the preservation of tissues and organs comprising carvedilol, tacrolimus, and trimetazidine. A synergistic effect is observed for this preservation solution which is particularly effective in marginal organs, such as steatotic livers. | 2013-03-07 |
| 20130059286 | CRYOPRESERVATION OF UMBILICAL CORD TISSUE FOR CORD TISSUE-DERIVED STEM CELLS - A method of preserving an umbilical cord is disclosed. The method comprises obtaining a segment of an umbilical cord; mincing the segment of the umbilical cord into cord tissue pieces; admixing the cord tissue pieces with a cryogenic composition comprising a cryoprotectant and a protein to form a mixture; shaking the mixture for a duration of no shorter than 20 minutes and no longer than 40 minutes; and cryopreserving the mixture. | 2013-03-07 |
| 20130059287 | Prolonging Survival of Platelets Using CMP-Sialic Acid, UDP-Galactose or Both - The present invention provides modified platelets having a reduced platelet clearance and methods for reducing platelet clearance. Also provided are compositions for the preservation of platelets. The invention also provides methods for making a pharmaceutical composition containing the modified platelets and for administering the pharmaceutical composition to a mammal to mediate hemostasis. | 2013-03-07 |
| 20130059288 | METHOD FOR ISOLATING TARGET CELLS - The present invention relates to a method for the enrichment and/or isolation of target cells in a sample, which sample comprises red blood cells and/or platelets, comprising (a) filtering the sample through a filter element having a pore or mesh size of between 0.5 and 5 μm, (b) contacting the cells retained by the filter element in step (a) with a separation surface, wherein said separation surface comprises affinity molecules which selectively bind to the target cells, (c) incubating the cells and the separation surface under conditions which allow for the binding of the affinity molecules to the target cells; and (d) separating the separation surface from any unbound cells and material. | 2013-03-07 |
| 20130059289 | ADENO-ASSOCIATED VIRUS SEROTYPE I NUCLEIC ACID SEQUENCES, VECTORS AND HOST CELLS CONTAINING SAME - The nucleic acid sequences of adeno-associated virus (AAV) serotype 1 are provided, as are vectors and host cells containing these sequences and functional fragments thereof. Also provided are methods of delivering genes via AAV-1 derived vectors. | 2013-03-07 |
| 20130059290 | DETECTION OF NUCLEIC ACIDS IN CRUDE MATRICES - A method includes contacting a crude matrix with components of an isothermal nucleic acid amplification reaction for a target nucleic acid species, thereby providing a mixture; incubating the mixture under conditions sufficient for the isothermal nucleic acid amplification reaction to proceed, thereby providing a product; and determining whether an indicator of the target nucleic acid species is present in the product. | 2013-03-07 |
| 20130059291 | COMPOSITIONS AND METHODS FOR IDENTIFYING DENGUE VIRUS - The invention generally relates to compositions and methods for identifying dengue virus. In certain embodiments, the invention provides methods for identifying a dengue virus serotype that involve obtaining a sample comprising at least one dengue virus protein; digesting the protein to produce peptides; ionizing the peptides; detecting a parent and associated fragment ion of the peptides; and identifying the serotype based on results of the detecting step. | 2013-03-07 |
| 20130059292 | METHOD OF DETECTING A TARGET USING APTAMER-MEDIATED PROTEIN PRECIPITATION ASSAY - The present invention relates to a method and detection kit for determining a presence of one or more proteins using aptamer-mediated pull-down assays. Specifically, a reproducible a protein precipitation (Co-AP/AP) method is provided to identify physiologically relevant protein-protein interactions by using target protein-specific aptamers, and to confirm its superior performance over antibody based protein precipitation (Co-IP/IP) methods in terms of its protein pull-down performance. | 2013-03-07 |
| 20130059293 | MAGNETIC RESONANCE SYSTEM AND METHOD TO DETECT AND CONFIRM ANALYTES - A system and method are provided to detect target analytes based on magnetic resonance measurements. Magnetic structures produce distinct magnetic field regions having a size comparable to the analyte. When the analyte is bound in those regions, magnetic resonance signals from the sample are changed, leading to detection of the analyte. | 2013-03-07 |
| 20130059294 | IDENTIFICATION OF POLYMORPHIC HEPATITIS B VIRUSES AND KRAS ONCOGENE MUTATIONS AND CLINICAL USE - The present application provides a method of monitoring patients of chronic hepatitis B virus (HBV) infection undergoing nucleoside/nucleotide analogue antiviral treatment for treatment efficacy and for risk of drug-resistance, by simultaneous determination of quantities of viral DNA and identification of mutant viruses responsible for drug-resistance. This invention also provides methods and reagents for highly sensitive identification/quantification of KRas oncogene mutations from body fluids or tumor tissues and the use of these methods for cancer risk assessment, cancer early detection, treatment outcome prediction, and treatment monitoring. | 2013-03-07 |
| 20130059295 | Host Cells and Methods for Producing Fatty Acid - The present invention provides for a genetically modified host cell capable of producing fatty acid comprising an increased expression of FadR, or a functional variant thereof. The host cell under environmental conditions wherein fatty acid is produced expresses an increased amount of FadR when compared to an unmodified host cell. The present invention also provides for a method of producing a fatty acid or FAAE in the host cell. The present invention provides for a genetically modified host cell comprising a fatty acid biosensor and one or more fatty acid-responsive promoter operably linked to one or more genes of interest that is heterologous to the fatty acid-responsive promoter. | 2013-03-07 |
| 20130059296 | Compositions and Methods For High Fidelity Assembly of Nucleic Acids - Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid. In certain embodiments, the plurality of blunt-end double-stranded nucleic acid fragments can be provided by: releasing a plurality of oligonucleotides synthesized on a solid support; and synthesizing complementary strands of the plurality of oligonucleotides using a polymerase based reaction. | 2013-03-07 |
| 20130059297 | METHOD FOR ISOLATING RENAL STEM/PROGENITOR CELLS, RENAL STEM/PROGENITOR CELLS AND THERAPEUTIC AGENT FOR RENAL DISEASE - It is intended to provide a method for noninvasively isolating human renal stem/progenitor cells, an isolated renal stem/progenitor cells, a therapeutic agent for renal disease, mouse mesenchymal cells which can be used for isolating human renal stem/progenitor cells and a culture supernatant of the same. Renal stem/progenitor cells are isolated by primarily culturing cells contained in the urine of a patient having renal disease in a medium containing mouse mesenchymal cells identified by the deposition number of FERM ABP-10865 or a culture supernatant of the same, staining the obtained primarily cultured cells with Hoechst 33342 and separating a weak-positive or negative fraction. | 2013-03-07 |
| 20130059298 | Composition and Method for Treatment of Tumors - The present invention relates to a composition which is useful in the treatment of a tumor, a method for making such a composition, and a method for using such a composition. The invention relates also to a method for assaying for inhibitors of the activity of Core 1 protein and/or other proteins of the respiratory complex III of mitochondria. | 2013-03-07 |
| 20130059299 | ABERRANT MITOCHONDRIAL DNA, ASSOCIATED FUSION TRANSCRIPTS AND TRANSLATION PRODUCTS AND HYBRIDIZATION PROBES THEREFORE - The present invention provides novel mitochondrial fusion transcripts, the parent mutated mtDNA molecules, and the resulting translation products (proteins) for predicting, diagnosing and/or monitoring cancer. Hybridization probes complementary thereto for use in the methods of the invention are also provided. | 2013-03-07 |
| 20130059300 | Insect Resistant Cotton Plants And Methods For Identifying Same - The invention provides specific transgenic cotton plants, plant material and seeds, characterized in that these products harbor a specific transformation event at a specific location in the cotton genome. Tools are also provided which allow rapid and unequivocal identification of the event in biological samples. | 2013-03-07 |
| 20130059301 | ASXL1 AS A NEW DIAGNOSTIC MARKER OF MYELOID NEOPLASMS - A method for diagnosing a myeloid cancer in a subject, includes the step of analyzing a biological sample from the subject by determining the presence or the absence of a mutation in the ASXL1 (additional sex combs like 1) gene coding for the polypeptide having the sequence SEQ ID N°2. A kit for diagnosing myeloid cancer in a subject including at least one nucleic acid probe or oligonucleotide or at least one antibody, which can be used in a such a method is also described. | 2013-03-07 |
| 20130059302 | NUCLEIC ACID DETECTION APPARATUS, METHOD AND COMPUTER READABLE RECORDING MEDIUM - A light reception section receives fluorescence emitted according to the amount of target nucleic acid that has been amplified by PCR due to a light source illuminating excitation light onto a reaction liquid. Electrical signals from the light reception section whose level depends on the received fluorescence intensity are amplified by plural amplification circuits having different amplification factors. A multiplexor selects an electrical signal amplified with an amplification factor in an initial stage of an amplification reaction and detects a fluorescence value for that cycle. A CPU acquires the largest fluorescence value in the initial stage and determines the amplification factor for a corrected stage and inputs a selection signal to the multiplexor to select the electrical signal amplified by the determined amplification factor. In the corrected stage the electrical signal amplified with the determined amplification factor is selected and fluorescence values detected. | 2013-03-07 |
| 20130059303 | CDKN2A AS A PROGNOSTIC MARKER IN BLADDER CANCER - The present invention provides methods for predicting clinical outcome and for providing information for determining follow-up strategy of a subject affected with a non-muscle invasive bladder cancer, as well as a method for selecting a subject affected with a non-muscle invasive bladder cancer for an anti-tumoral therapy. The present invention also provides kits for implementing these methods. | 2013-03-07 |
| 20130059304 | TRANSLOCATION AND MUTANT ROS KINASE IN HUMAN NON-SMALL CELL LUNG CARCINOMA - In accordance with the invention, a novel gene translocation, (4p15, 6q22), in human non-small cell lung carcinoma (NSCLC) that results in a fusion proteins combining part of Sodium-dependent Phosphate Transporter Isoform NaPi-3b protein (SLC34A2) with Proto-oncogene Tyrosine Protein Kinase ROS Precursor (ROS) kinase has now been identified. The SLC34A2-ROS fusion protein is anticipated to drive the proliferation and survival of a subgroup of NSCLC tumors. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ROS kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of the new fusion protein enables new methods for determining the presence of these mutant ROS kinase polypeptides in a biological sample, methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides, which are also provided by the invention. | 2013-03-07 |
| 20130059305 | TRANSLOCATION AND MUTANT ROS KINASE IN HUMAN NON-SMALL CELL LUNG CARCINOMA - In accordance with the invention, a novel gene translocation, (4p15, 6q22), in human non-small cell lung carcinoma (NSCLC) that results in a fusion proteins combining part of Sodium-dependent Phosphate Transporter Isoform NaPi-3b protein (SLC34A2) with Proto-oncogene Tyrosine Protein Kinase ROS Precursor (ROS) kinase has now been identified. The SLC34A2-ROS fusion protein is anticipated to drive the proliferation and survival of a subgroup of NSCLC tumors. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ROS kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of the new fusion protein enables new methods for determining the presence of these mutant ROS kinase polypeptides in a biological sample, methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides, which are also provided by the invention. | 2013-03-07 |
| 20130059306 | PRIMER AND PROBE FOR DETECTING CHLAMYDIA TRACHOMATIS, AND METHOD FOR DETECTING CHLAMYDIA TRACHOMATIS USING SAME - The present invention relates to an oligonucleotide which is designed on the basis of a nucleotide sequence shown in SEQ ID NO: 1 or SEQ ID NO: 2 and hybridizes with the endogenous plasmid gene of | 2013-03-07 |
| 20130059307 | Methods And Compositions For Detecting Fungi And Mycotoxins - The invention relates to a method of identifying a specific fungal species in patient tissue or body fluid. The method comprises the steps of extracting and recovering DNA of the fungal species from the patient tissue or body fluid, amplifying the DNA, hybridizing a probe to the DNA to specifically identify the fungal species, and specifically identifying the fungal species. The invention also relates to a method of identifying a mycotoxin in patient tissue or body fluid. The method comprises the steps of extracting and recovering the mycotoxin from the patient tissue or body fluid, contacting the mycotoxin with an antibody directed against the mycotoxin, and identifying the myocotoxin. Both of these methods can be used to determine if a patient is at risk for or has developed a disease state related to a fungal infection, and to develop an effective treatment regimen for the patient. | 2013-03-07 |
| 20130059308 | POLYMER MICROFILTERS AND METHODS OF MANUFACTURING THE SAME - A microfilter comprising a polymer layer formed from epoxy-based photo-definable dry film, and a plurality of apertures each extending through the polymer layer. A method of forming a microfilter is also disclosed. The method includes providing a first layer of epoxy-based photo-definable dry film disposed on a substrate, exposing the first layer to energy through a mask to form a pattern, defmed by the mask, in the first layer of dry film, forming, from the exposed first layer of dry film, a polymer layer having a plurality of apertures extending therethrough, the plurality of apertures having a distribution defined by the pattern, and removing the polymer layer from the substrate. | 2013-03-07 |
| 20130059309 | Dopaminergic Neurons Derived From Induced Pluripotent Stem Cells and Method of Making Same - Provided are compositions and methods that relate to cultured neurons. The cultured neurons can either have or not have genetic mutations that are characteristic of Parkinson's disease (PD). The cultured neurons are generated from induced pluripotent stem cells made from human fibroblasts that are obtained from individuals with and without PD. Cultured neurons without genetic mutations characteristic of PD are dopaminergic neurons that exhibit specific dopamine uptake are provided. Also provided is a method for identifying a test agent as a potential candidate for reducing the severity of PD. The method involves obtaining cells of neural lineage derived from cells obtained from an individual who has PD and measuring the effects of the test agents on dopaminer-characteristics, including specific dopamine uptake, monoamine oxidase (MAO) transcription levels, protein and/or activity levels of estrogen-related receptors, and combinations thereof. An increase in specific dopamine uptake, inhibition of MAO transcription or decrease in the level and/or activity of estrogen-related receptors caused by the agent can be used to identify the agent as a potential candidate for reducing the severity of PD. | 2013-03-07 |
| 20130059310 | Compositions and Methods for Intramolecular Nucleic Acid Rearrangement - Aspects of the present invention are drawn to processes for moving a region of interest in a polynucleotide from a first position to a second position with regard to a domain within the polynucleotide, also referred to as a “reflex method”. In certain embodiments, the reflex method results in moving a region of interest into functional proximity to specific domain elements present in the polynucleotide (e.g., primer sites and/or MID). Compositions, kits and systems that find use in carrying out the reflex processes described herein are also provided. | 2013-03-07 |
| 20130059311 | METHOD FOR THE IDENTIFICATION OF MEMORY MODULATING COMPOUNDS BY ASSESSING KIBRA EXPRESSION - Provided are cell based methods to identify memory modulating compounds based on the assessment of KIBRA expression. These methods are also suitable to uncover memory related pathways. The methods comprise the contacting of test compounds with cultured cells and the subsequent assessment of modulation of the KIBRA expression within the cells. | 2013-03-07 |
| 20130059312 | Methods Of Using Chimeric Receptors to Identify Autoimmune Disease - The present invention provides methods and compositions useful in the diagnosis and management of autoimmune diseases. In particular, the present invention provides improved methods and compositions for the diagnosis and management of Graves' disease. The methods of the present invention not only avoids the need for radioactivity and are much simpler, economical, and rapid than methods traditionally used for the diagnosis of Graves' disease, but also improve upon the sensitivity and detection abilities of previous luciferase-based autoantibody detection assays. | 2013-03-07 |
| 20130059313 | ANALYTE QUANTIFICATION USING FLOW INDUCED DISPERSION ANALYSIS - A method for quantitative characterization of non-covalent interactions and analyte quantification in nanoliter samples is described. The procedure is based on Flow Induced Dispersion Analysis (FIDA), of which the only system requirements is a narrow tube, capillary or channel equipped with a detector. The technique can be implemented using standard equipment such as High Performance Liquid Chromatography (HPLC), Flow Injection Analysis (FIA) or Capillary Electrophoresis (CE). | 2013-03-07 |
| 20130059314 | FRET-BASED METHOD FOR THE DETERMINATION OF PROTEIN PHOSPHATASE AND KINASE ACTIVITY - This disclosure relates to methods of determining activities of protein phosphatases and kinases. The disclosure further relates to methods of clinical monitoring of calcineurin activity and immunosuppression in patients and which may be used to predict transplant acceptance in patients. | 2013-03-07 |
| 20130059315 | Alpha-Amylase Mutants - The present invention relates to a method of constructing a variant of a parent Termamyl-like alpha-amylase, which variant has alpha-amylase activity and at least one altered property as compared to the parent alpha-amylase, comprises
| 2013-03-07 |
| 20130059316 | PLASMONIC ELECTRICITY - The present invention relates to detection systems and methods that detect fluorescence, luminescence, chemiluminescence or phosphorescence signatures in the form of an electrical signal conducted and emitted from metallic containing surfaces. Thus, the present invention provides for detecting fluorescence digitally and directly without the need for expensive detectors. | 2013-03-07 |
| 20130059317 | Binding Partners for the Thyrotropin Receptor and Uses Thereof - A binding partner for the TSH receptor, which binding partner comprises, or is derived from, a human monoclonal or recombinant antibody, or one or more fragments thereof, reactive with the TSH receptor, uses thereof, methods of diagnosis and therapy employing the same, and anti-idiotypic antibodies thereto. | 2013-03-07 |
| 20130059318 | ANTI-IGA1 ANTIBODY - An object of the present invention is to provide a monoclonal antibody which is effective for diagnosing IgA nephropathy and specifically recognizes and binds to a hinge region of a polypeptide encoded by a heavy chain gene of immunoglobulin A1 that comprises a serine/threonine-linked sugar chain to which galactose is not bound. According to the present invention, it is possible to provide a monoclonal antibody or an antibody fragment thereof that specifically recognizes and binds to a hinge region of a polypeptide encoded by a heavy chain gene of immunoglobulin A1 that comprises a serine/threonine-linked sugar chain to which galactose is not bound, to provide a diagnostic agent using the antibody or the antibody fragment thereof, and to provide a therapeutic agent comprising the antibody or the antibody fragment thereof as an active ingredient. | 2013-03-07 |
| 20130059319 | METHOD AND A DEVICE FOR DETECTING, CLASSIFYING, AND IDENTIFYING PARTICLES, AEROSOLS, AND/OR VAPORS IN THE AIR - A method and device are disclosed for continuously detecting, classifying and identifying toxic particles, aerosols and/or vapor in an air sample, in near real time by directing an air sample containing an optional target analyte, in the form of particles, aerosols and/or vapors, enzyme(s), and enzyme substrate(s), to a surface of a collection matrix for forming a biocatalytic reaction product of a plurality of freely mobile optical reporters, and by using a light source with optical reader to interpret the signal from the optical reporter, enabling the detection, classification and identification of toxic particles, aerosols and/or vapor in the air sample. | 2013-03-07 |
| 20130059320 | Selective growth media for campylobacter bacteria and plating media with said growth media - A selective growth media for | 2013-03-07 |
| 20130059321 | Labeling of Proteins with the Fluorophore 7-amino-4-methylcoumarin (AMC) Generated Novel Proteolytic Substrates - A method of measuring the degradation of intact proteins includes a step of providing a protein substrate having one or more free or exposed carboxyl groups and then reductively attaching 7-amino-4-methylcoumarin (AMC) to the protein substrate with a reducing agent. The protein substrate is contacted in a test solution with one or more proteolytic enzymes that degrade the protein substrate. The amount of AMC attached to the protein substrate is then determined by monitoring the fluorescence of free 7-amino-4-methylcoumarin that is formed during degradation of the protein substrate to protein fragments. | 2013-03-07 |
| 20130059322 | CELL CULTURE AND INVASION ASSAY METHOD AND SYSTEM - Microfluidic devices, systems, and methods providing for an invasion assay using microfluidic culture systems. | 2013-03-07 |
| 20130059323 | Detection of Overtraining Syndrome in an Individual - The onset of or the existence of overtraining syndrome in an individual is detected by a method which comprises a) contacting leucocytes in, or obtained from, a blood sample provided by the individual with a luminescence reagent which emits light on reaction with an oxidant; b) adding an activator to the mixture of leucocytes and the luminescence reagent; c) continuously monitoring and/or measuring light emitted by the luminescence reagent over a predetermined time period commencing before and ending after the addition of the activator; and d) assessing the light emission. The individual may be a human, for example an elite athlete, or a non-human mammal, for example a racehorse. A diagnostic kit, for carrying out the method, comprising a luminescence reagent which emits light on reaction with an oxidant, an activator and a library of standard signature light emission curves is also disclosed. | 2013-03-07 |
| 20130059324 | SYSTEMS AND METHODS FOR MECHANICALLY STRAINED CELL CULTURE - A method for in vitro drug screening can include: providing one or more cells in vitro; introducing a chemical to the one or more cells; introducing a mechanical strain to the one or more cells in the presence of the chemical; and determining whether or not the chemical has bioactivity with respect to the one or more cells under the mechanical strain. The mechanical strain can be implemented with a well plate defining a plurality of cell culture wells and having one or more flexible substrates associated with the well plate so that each cell culture well has a fluid tight flexible cell culture substrate. A dynamic in vitro screening device can include: a top plate defining one or more cell culture wells; one or more flexible cell culture substrates operably coupled with the top plate to form fluid tight cell culture well bottoms; an a pin plate under the top plate with the one or more flexible cell culture substrates therebetween, the pin plate having one or more pin members associated with the one or more cell culture wells. | 2013-03-07 |
| 20130059325 | ISOLATED POPULATIONS OF ADULT RENAL CELLS AND METHODS OF ISOLATING AND USING SAME - A method of generating a nephrospheroid is disclosed. The method comprises culturing human adult kidney cells in a culture medium under non-adherent conditions. Uses thereof and other renal cell populations are also disclosed. | 2013-03-07 |
| 20130059326 | Membrane Transfer Method And Tool - A method for transferring a filter membrane ( | 2013-03-07 |
| 20130059327 | Automatic Toxicological Fluid Sample Preparation Module, Automatic Analysis System and Method for Use Thereof - This present invention relates to an automatic toxicological fluid sample preparation module and method for use thereof, for automatically preparing a fluid sample for the detection and measurement of toxics and/or toxicological effects. The invention further relates to an automatic analysis system. The automatic toxicological fluid sample preparation module comprising: an inlet for automatically obtaining a sample directly from a fluid or fluid stream to be analyzed; preparation for preparing the fluid sample from the sample, comprising first coupling means for coupling with the inlet; and—an outlet for discharge of prepared fluid to measurement means, wherein the preparation means comprise indicator micro-organisms. | 2013-03-07 |
| 20130059328 | PRODUCTION OF ENTERITIS EVOKING CAUSAL FACTOR BY HIGHLY PATHOGENIC ORAL BACTERIA AND HIGH SENSITIVITY DETECTION METHOD THEREFOR - Disclosed is the construction of a system capable of quickly and easily identifying patients having a risk for worsening inflammatory digestive tract disease by identifying a causal factor for the worsening of the inflammatory digestive tract disease. Specifically disclosed is a method for detecting oral bacteria that cause worsening of inflammatory digestive tract disease and/or a screening that targets high risk for worsening of inflammatory digestive tract disease and/or determining the risk for worsening of the inflammatory digestive tract disease by PA not being detected and/or CBP being detected, inclusive of the detection of PA, which is an oral bacteria protein antigen, and/or CBP, which is collagen binding protein, in samples. Also disclosed are a detection reagent and kit used for this method. | 2013-03-07 |
| 20130059329 | APPARATUS AND METHOD FOR DISTRIBUTING LIQUID SAMPLES IN SMALL VOLUMES - Apparatus for dispensing liquids in small sample volumes, comprising a preformed rigid duct of impermeable material, with one inlet and one or more outlets, characterized in that the duct dimensions vary along it, forming communicating ducts, bifurcations and reservoirs; and a process for its manufacture. | 2013-03-07 |
| 20130059330 | FIXING SOLUTION FOR BIOLOGICAL CELLS - This fixing solution is intended to preserve in vitro a cytological sample, comprising nucleated cells and erythrocytes and comprises alcohol for fixing the cells. It comprises physiological saline in order to avoid osmotic shocks at the wall of the cells, formalin and polyethylene glycol for retaining the size and the integrity of the nucleated cells and erythrocytes, which are fixed in said solution. | 2013-03-07 |
| 20130059331 | CELL SUITABLE FOR FERMENTATION OF A MIXED SUGAR COMPOSITION - The present invention relates to a cell suitable for production of one or more fermentation product from a sugar composition comprising glucose, galactose, arabinose and xylose, wherein the cell comprises two to fifteen copies of one or more xylose isomerase gene or two to fifteen copies of one or more xylose reductase and xylitol dehydrogenase, and two to ten copies of araA, araB and araD, genes, wherein these genes are integrated into the cell genome. | 2013-03-07 |
| 20130059332 | METHOD FOR THE PRODUCTION OF AN AQUEOUS GLUCOSE SOLUTION - The present invention relates to a process for the production of an aqueous glucose solution from maize or maize kernels. The invention also relates to a glucose solution obtainable by this process, and to its use for the production of organic compounds. The process according to the invention comprises: a) fractionating dry milling of maize kernels, where the maize kernels are separated into a maize-starch-comprising endosperm fraction and a high-oil germ fraction and, if appropriate, a bran fraction; b) enzymatic liquefaction and saccharification of the maize starch in an aqueous suspension of the endosperm fraction, which gives an aqueous glucose solution comprising maize gluten; and c) depletion of the maize gluten and, if appropriate, any bran present from the aqueous glucose solution. | 2013-03-07 |
| 20130059333 | HEMATOPOIETIC CELL SELECTIN LIGAND POLYPEPTIDES AND METHODS OF USE THEREOF - The invention feature methods and compositions for treating inflammatory disorders, hematopoietic disorders and non-hematopoietic disorders (e.g., non-hematopoietic cancers) and for isolating cells (e.g., stem cells) in a mammal. | 2013-03-07 |
| 20130059334 | PRODUCTION OF RECOMBINANT SELENOPROTEIN MUTANTS WITH ENHANCED CATALYTIC ACTIVITY - The present invention generally relates to the production of industrially relevant quantities of selenoprotein enzymes in eukaryotic cell cultures. More specifically, the present invention generally relates to the production of such enzymes wherein one or more catalytic cysteine or serine residues are mutagenically replaced by selenocysteine. | 2013-03-07 |
| 20130059335 | USE OF A GENETICALLY MODIFIED CELL LINE EXPRESSING FUNCTIONAL ASIALOGLYCOPROTEIN RECEPTOR IN THE PRODUCTION OF SIALYLATED GLYCOPROTEINS - The present invention is directed to the use of a cell line in the production of sialylated glycoprotein, wherein said cell line expresses functional ASGPR protein as well as to a method for the production of sialylated glycoproteins, characterized in that such a cell line is used. | 2013-03-07 |
| 20130059336 | HYDROPHOBIC INTERACTION CHROMATOGRAPHY METHOD - Herein is reported a method for purifying a polypeptide comprising a histidine-tag comprising the steps of i) applying a solution comprising the polypeptide with a histidine-tag to a hydrophobic interaction chromatography material, and ii) recovering the polypeptide comprising a histidine-tag with a solution comprising imidazole or an imidazole-derivative and thereby purifying the polypeptide comprising a histidine-tag, wherein the solution comprising the polypeptide applied to the hydrophobic interaction chromatography material is free of imidazole or an imidazole-derivative and the polypeptide adsorbed to the hydrophobic interaction chromatography material is recovered with a solution comprising imidazole or an imidazole-derivative. | 2013-03-07 |
| 20130059337 | Therapeutic Uses of Humanized Antibodies Against ALPHA-4 Integrin - The invention provides method of treatment using humanized immunoglobulins that specially bind to alpha-4 integrin. The methods are useful for treatment of asthma, atherosclerosis, AIDS dementia, diabetes, inflammatory bowel disease, rheumatoid arthritis, transplant rejection, graft versus host disease, tumor metastasis, nephritis, atopic dermatitis, psoriasis, myocardial ischemia, and acute leukocyte mediated lung injury. | 2013-03-07 |
| 20130059338 | ARTIFICIAL ENTROPIC BRISTLE DOMAIN SEQUENCES AND THEIR USE IN RECOMBINANT PROTEIN PRODUCTION - Compositions and methods for recombinant protein production and, more particularly, fusion polypeptides, polynucleotides encoding fusion polypeptides, expression vectors, kits, and related methods for recombinant protein production. | 2013-03-07 |
| 20130059339 | Apparatus and Methods for Cell Culture - A bioreactor ( | 2013-03-07 |
| 20130059340 | BIOCATALYTIC PRODUCTION OF GLYCOSIDES - The present invention relates to the biocatalytic production of glycosides. In particular, the invention discloses a method to produce alkylcellobiosides, arylcellobiosides, cellobiolipids, cellotriolipids, glucosophorolipids and cellobiosesophorolipids via the usage of a cellodextrin phosphorylase (CDP) derived from | 2013-03-07 |
| 20130059341 | METHOD FOR PRODUCING GLUCURONIC ACID CONJUGATE USING SACCHAROMYCES CEREVISIAE - Provided is a new method for producing a glucuronide, having excellent productivity and being replaceable with a method using | 2013-03-07 |
| 20130059342 | COMPOSITIONS AND METHODS FOR USE IN RECOMBINATIONAL CLONING OF NUCELIC ACIDS - The present invention relates to compositions and methods for use in recombinational cloning of nucleic acid molecules. In particular, the invention relates to nucleic acid molecules encoding one or more recombination sites or portions thereof, to nucleic acid molecules comprising one or more of these recombination site nucleotide sequences and optionally comprising one or more additional physical or functional nucleotide sequences. The invention also relates to vectors comprising nucleic acid molecules of the invention, to host cells comprising vectors or nucleic acid molecules of the invention, to methods of producing polypeptides using nucleic acid molecules of the invention, and to polypeptides encoded by these nucleic acid molecules or produced by methods of the invention. The invention also relates to the use of these compositions in methods for recombinational cloning of nucleic acids, in vitro and in vivo, to provide chimeric DNA molecules that have particular characteristics and/or DNA segments. | 2013-03-07 |
| 20130059343 | NUCLEOTIDE DERIVATIVES - The present invention provides compounds and methods for attaching fluorescent labels to biological molecules such as nucleotides. The compounds and methods are useful for biological assays including DNA modification reactions. | 2013-03-07 |
| 20130059344 | TRANSCRIPTION TERMINATOR SEQUENCES - The present invention provides new transcription termination signal sequences, especially a polynucleotide comprising at least two consecutive transcription termination signals, characterized in that said consecutive transcription termination signals comprise at least a first and a second transcription termination signal that are at most 1000 nucleotides apart, and at least one of the termination signal has or encodes a RNA hairpin structure. | 2013-03-07 |
| 20130059345 | MANUFACTURING METHOD FOR SUGAR SOLUTION AND DEVICE FOR SAME - A method of hydrolysis of cellulose uses a filamentous fungus-derived cellulase as a carbohydrase and includes adding the carbohydrase to cellulose to perform primary hydrolysis and then subjecting the primary hydrolysate to solid-liquid separation into a primary sugar liquid and solids; adding water to the solids and performing secondary hydrolysis, followed by subjecting the secondary hydrolysate to solid-liquid separation into a secondary sugar liquid and a residue; and filtering the primary sugar liquid and/or secondary sugar liquid through an ultrafiltration membrane, and recovering the carbohydrase from the feed side and recovering a sugar solution from the permeate side. | 2013-03-07 |
| 20130059346 | Conversion System For Biomass - The efficient production of ethanol from low-cost biomass (e.g., corn, sugar beets, sugar cane, switchgrass and/or paper) has become increasingly important in making ethanol competitive with gasoline and decreasing the United States' dependence on foreign oil. For example, to reduce the cost of transporting biomass to ethanol production facilities, mobile systems for producing ethanol from biomass are provided. Also provided are small-scale ethanol production facilities. For example, instead of transporting biomass to the production facility, the facility is transported to the biomass or is located nearby the source of the biomass. The ethanol production facilities or components thereof may be transported via land, water, or air. Production of other products, such as hydrocarbons, natural gas, hydrogen gas, plastics, polymers, and proteins, can also be made by the methods and facilities. Any product described herein can be made in finished form or un-finished form and moved, e.g., to a fixed facility, e.g., fixed production facility. | 2013-03-07 |
| 20130059347 | SACCHARIFIED-SOLUTION MANUFACTURING METHOD AND SACCHARIFIED-SOLUTION MANUFACTURING DEVICE USED IN SAID METHOD - [Problems to be Solved] | 2013-03-07 |
| 20130059348 | NOVEL AMIDASE, GENE FOR THE SAME, VECTOR, TRANSFORMANT, AND METHOD FOR PRODUCTION OF OPTICALLY ACTIVE CARBOXYLIC ACID AMIDE AND OPTICALLY ACTIVE CARBOXYLIC ACID BY USING ANY ONE OF THOSE ITEMS - The present invention has its object to provide a novel polypeptide having amidase activity to selectively hydrolyze S-enantiomer in racemic nipecotamide, a DNA encoding the polypeptide, a vector containing the DNA, a transformant transformed with the vector, and a method for producing an optically active carboxylic acid amide and an optically active carboxylic acid in which a racemic carboxylic acid amide is hydrolyzed with the polypeptide or the transformant. | 2013-03-07 |
| 20130059349 | METHOD FOR PRODUCING ACRYLAMIDE USING MICROBIAL CATALYST - The present invention provides a more efficient method for producing acrylamide from acrylonitrile by the action of a microbially-derived enzyme, nitrile hydratase. More specifically, the present invention provides a method for producing acrylamide from acrylonitrile using a biocatalyst having nitrile hydratase, which comprises the step of keeping acrylonitrile while cooling to less than 30° C. Moreover, the present invention also provides an apparatus for producing acrylamide from acrylonitrile using a biocatalyst having nitrile hydratase. | 2013-03-07 |
| 20130059350 | CONSTRUCTS AND METHODS FOR INCREASING YIELD OF FATTY ALCOHOLS IN CYANOBACTERIA - A construct containing a gene operably linked to a promoter having activity in cyanobacteria, and vector and cyanobacterium comprising such construct for increasing the yield of fatty alcohols in cyanobacterium is provided. | 2013-03-07 |
| 20130059351 | Thioesterase and a Method of Producing Fatty Acids or Lipids Using the Thioesterase - A thioesterase comprising an amino acid sequence set forth in SEQ ID NO: 1, a thioesterase gene encoding the thioesterase, a transformant comprising the gene, and a method of producing fatty acids or lipids using the transformant. | 2013-03-07 |
| 20130059352 | Alcohol Production Process - The invention relates generally to methods for improving the microbial fermentation of gaseous substrates to produce one or more products. The invention relates to the capture of one or more components of a gaseous stream in a liquid. The invention relates to the deoxygenation of a liquid by contacting the liquid with a gaseous stream. The invention relates to methods of increasing efficiency of fermentation by using gas streams exiting a bioreactor to deoxygenate liquid streams entering a bioreactor. | 2013-03-07 |
| 20130059353 | Recombinant Microorganisms for Production C4-Dicarboxylic Acids - Provided herein are host cells comprising carbonic anhydrase activity, wherein the cells are capable of producing C4-dicarboxylic acid. Also provided are methods of producing C4-dicarboxylic acid comprising (a) cultivating the host cells having carbonic anhydrase activity in a medium under suitable conditions to produce C4-dicarboxylic acid; and (b) recovering the C4-dicarboxylic acid. | 2013-03-07 |
| 20130059354 | ALKANOL - The present invention provides a process for the production of an alkanol (e.g. butanol) comprising converting a carbon source such as a biomass into a carboxylic acid or a derivative thereof using a microorganism, and electrochemically reducing said acid to said alkanol, as well as an apparatus for carrying out said process. | 2013-03-07 |
| 20130059355 | THERMOPHILIC MICRO-ORGANISMS FOR ETHANOL PRODUCTION - A thermophilic micro-organism comprising a modification that increases amylase expression and starch hydrolysis compared to wild-type, wherein the modification is insertion of a heterologous amylase gene. | 2013-03-07 |
| 20130059356 | ETHANOL PRODUCTION FROM MANNITOL USING YEAST - This invention provides a method for producing ethanol from mannitol using yeast and a yeast strain that produces ethanol from mannitol. The method for producing ethanol from mannitol comprises culturing yeast strains capable of mannitol assimilation and ethanol production from mannitol in a medium containing mannitol. | 2013-03-07 |
| 20130059357 | Process for producing biogas - Process for the production of a biogas containing methane from an organic matter amenable to anaerobic digestion comprising feeding an anaerobic digester with the organic matter, said anaerobic digester containing a digestion medium comprising microorganisms capable of digesting said organic matter, wherein the total inorganic carbon concentration of the digestion medium is maintained above 9000 mg of equivalent CaCO | 2013-03-07 |
| 20130059358 | IN-SITU ELECTRICAL STIMULATION OF BIOCONVERSION OF CARBON-BEARING FORMATIONS - Methods of stimulating microbial consortia, such as microbial consortia in a geological formation, such as comprising methanogens and other bacteria, for producing methane and other fuels or fuel precursors from coal or other carbonaceous materials, are disclosed along with methods for increasing bioconversion of carbonaceous materials, such as coal, into methane and other useful hydrocarbon products, wherein the consortia respond to electrical stimulation, either physical or chemical. | 2013-03-07 |
| 20130059359 | Protein Nanorings - The invention provides protein nanorings. | 2013-03-07 |
| 20130059360 | POLYMER-BASED COMPOSITIONS AND CONJUGATES OF ANTIMICROBIAL AGENTS - Provided herein are water-soluble polymer conjugates and polymer-based compositions of antimicrobial agents. Also provided are methods for synthesizing and administering such conjugates and compositions. | 2013-03-07 |
| 20130059361 | Compositions and Methods for Effecting the Levels of High Density Lipoprotein (HDL) Cholesterol and Apolipoprotein AI, Very Low Density Lipoprotein (VLDL) Cholesterol and Low Density Lipoprotein (LDL) Cholesterol - Compositions and methods for raising the level of HDL cholesterol and apolipoprotein AI in a patient and for lowering the levels of VLDL cholesterol and LDL cholesterol in a patient, including compositions and methods which effect the expression of a gene, LIPG, which encodes a lipase enzyme that is a member of the triacylglycerol lipase family or which effect the enzymatic activity of the enzyme. | 2013-03-07 |
| 20130059362 | BIOSENSORS COMPRISING PROTEIN-BINDING DOMAINS AND FLUORESCENT PROTEINS - The present invention provides compositions and methods for monitoring cellular signal transduction events. In particular, signal transduction events may be monitored by the use of biosensors comprising protein-binding domains, which bind signal-transduction proteins, and fluorescent proteins. Provided herein, therefore, methods for detecting activation of signal transduction proteins or screening for agents that modulate the activity of signal transduction proteins. Also provided are cells comprising the biosensors; lentiviral particles comprising biosensor coding sequence; and kits comprising the lentiviral particles. | 2013-03-07 |
| 20130059363 | Isolated Nucleic Acids for Producing High Viscosity Diutan Gums and Methods - The production of a diutan polysaccharide exhibiting increased viscosity properties as compared with previously produced polysaccharide of the same type of repeating units. Such an improved diutan polysaccharide is produced through the generation of a derivative of | 2013-03-07 |
| 20130059364 | CULTIVATION OF DISPERSED MYCOBACTERIA - The present invention relates to a culture medium for disperse mycobacteria comprising at least one detergent and an antifoam agent, whereby said antifoam agent is a member of the silicone family. In addition, the invention provides for methods for the activation of dipressed mycobacteria, for obtaining a standardized mycobyterial culture or for the production of (a), purified protein derivates(s) (PDD) which comprise the cultivation of mycobacteria in the active medium disclosed herein. Furthermore, the use of standardized mycobacterial cultures obtained by the method of the invention for the preparation of pharmaceutical or diagnostic compositions is described. | 2013-03-07 |
| 20130059365 | Target Gas Capture - Capturing a target gas includes contacting a gas mixture including a target species with an aqueous solution including a buffer species, and transferring some of the target species from the gas mixture to the aqueous solution. The target species forms a dissolved target species in the aqueous solution, and the aqueous solution is processed to yield a first aqueous stream and a second aqueous stream, where the equilibrium partial pressure of the target species over the second aqueous stream exceeds the equilibrium partial pressure of the target species over the first aqueous stream. At least some of the dissolved target species in the second aqueous stream is converted to the target species, and the target species is liberated from the second aqueous stream. The target species can be collected and/or compressed for subsequent processing or use. | 2013-03-07 |
| 20130059366 | Integrated Droplet Actuator for Gel; Electrophoresis and Molecular Analysis - The invention is directed to a droplet actuator device and methods for integrating gel electrophoresis analysis with pre or post-analytical sample handling as well as other molecular analysis processes. Using digital microfluidics technology, the droplet actuator device and methods of the invention provide the ability to perform gel electrophoresis and liquid handling operations on a single integrated device. The integrated liquid handling operations may be used to prepare and deliver samples to the electrophoresis gel, capture and subsequently process products of the electrophoresis gel or perform additional assays on the same sample materials which are analyzed by gel electrophoresis. In one embodiment, one or more molecular assays, such as nucleic acid (e.g., DNA) quantification by real-time PCR, and one or more sample processing operations such as sample dilution is performed on a droplet actuator integrated with an electrophoresis gel. In one embodiment, an electrophoresis gel may be integrated on the top substrate of the droplet actuator. | 2013-03-07 |
| 20130059367 | ULTRASENSITIVE BIOSENSORS - The present invention is a biosensor apparatus that includes a substrate, a source on one side of the substrate, a drain spaced from the source, a conducting channel between the source and the drain, an insulator region, and receptors on a gate region for receiving target material. The receptors are contacted for changing current flow between the source and the drain. The source and the drain are relatively wide compared to length between the source and the drain through the conducting channel. | 2013-03-07 |
| 20130059368 | SYSTEM FOR CULTURING AND RECOVERING MICRO ALGAE - A system for culturing and recovering micro algae comprises a photo-bioreactor, a floatation separator, a centrifugal separator, and a micro bubble generator. The photo-bioreactor unit is configured to culture micro algae by a photochemical reaction to produce a micro algae precipitate. The precipitated micro algae is separated by the floatation separator. The separated micro algae is concentrated by the centrifugal separator. The micro bubble generator generates process water containing micro carbon dioxide bubbles and supplies the generated process water to the photo-bioreactor unit and the floatation separator. With this system, micro algae can be cultured and recovered in a simper and more cost-effective manner. | 2013-03-07 |
| 20130059369 | MICROALGAE CULTIVATION MODULE - A microalgae cultivation module for carbon reduction and biomass production is provided, which includes a first photobioreactor set, a second photobioreactor set, a gas switching device and a control unit. The gas switching device is communicated to the first and the second photobioreactor sets. The control unit is coupled to and controls the gas switching device, thereby aerating a waste gas into the first photobioreactor set and aerating air into the second photobioreactor set for a first predetermined time, then aerating the waste gas into the second photobioreactor set and aerating the air into the first photobioreactor set for a second predetermined time. The first and the second photobioreactor sets include a microalgae species. | 2013-03-07 |
| 20130059370 | RECOVERY OF HIGHER ALCOHOLS FROM DILUTE AQUEOUS SOLUTIONS - This invention is directed to methods for recovery of C3-C6 alcohols from dilute aqueous solutions, such as fermentation broths. Such methods provide improved volumetric productivity for the fermentation and allows recovery of the alcohol. Such methods also allow for reduced energy use in the production and drying of spent fermentation broth due to increased effective concentration of the alcohol product by the simultaneous fermentation and recovery process which increases the quantity of alcohol produced and recovered per quantity of fermentation broth dried. Thus, the invention allows for production and recovery of C3-C6 alcohols at low capital and reduced operating costs. | 2013-03-07 |
| 20130059371 | Device, System and Process for Modification or Concentration of Cell-depleted Fluid - A product concentration device that utilizes a reservoir connected to a hollow-fiber filter element where the reservoir can serve as a container for filtrate emanating from another filtering device, such that product in the reservoir can be stored, concentrated and/or further processed as desired. Enclosed reactor systems, each of at least three chambers, fluid flow between the chambers controlled by selectively permeable barriers, flow controlled by an alternating flow diaphragm pump. | 2013-03-07 |
| 20130059372 | SAMPLE PROCESSING UNITS, SYSTEMS, AND RELATED METHODS - Sample processing units useful for mixing and purifying materials, such as fluidic materials are provided. A sample processing unit typically includes a container configured to contain a sample comprising magnetically responsive particles, and one or more magnets that are in substantially fixed positions relative to the container. A sample processing unit also generally includes a conveyance mechanism configured to convey the container to and from a position that is within magnetic communication with the magnet, e.g., such that magnetically responsive particles with captured analytes can be retained within the container when other materials are added to and/or removed from the container. Further, a sample processing unit also typically includes a rotational mechanism that is configured to rotate the container, e.g., to effect mixing of sample materials disposed within the container. Related carrier mechanisms, sample processing stations, systems, and methods are also provided. | 2013-03-07 |
| 20130059373 | ENDOMETRIAL POLYP STEM CELL - An endometrial polyp stem cell is disclosed in the present invention. The endometrial polyp stem cell is isolated from an endometrial polyp tissue, expresses vimentin, CD13, CD29, CD44, and CD90, and has no expression of CD1q, CD3, CD34, and CD45. | 2013-03-07 |
| 20130059374 | Novel Therapeutical Tools and Methods for Treating Blindness - The present inventions relates to the use of an isolated nucleic acid molecule comprising a nucleotide sequence coding for a hyperpolarizing light-gated ion channel or pump gene from an archeon or for a light-active fragment of said gene, or the nucleotide sequence complementary to said nucleotide sequence, for treating or ameliorating blindness. The light-gated ion channel or pump gene can be a halorhodopsin gene. | 2013-03-07 |
| 20130059375 | METHOD FOR PREPARING ES CELLS - The present invention provides a method of producing a mammalian ES cell, including cultivating a mammalian inner cell mass in a medium containing adrenocorticotropic hormone, an FGF receptor inhibitor, an MEK activation inhibitor and a GSK3 inhibitor, and isolating a mammalian ES cell from the culture. | 2013-03-07 |
| 20130059376 | CULTURE VESSEL AND METHOD OF GROWING CELLS IN CULTURE VESSEL - A culture vessel and a method of growing cells in a culture vessel. The culture vessel includes a container and a plurality of agitators. The container receives a medium and cells. The plurality of agitators is positioned to contact the medium when received in the container and configured to promote suspension of the cells in the medium upon oscillation of the agitators. The agitators are at least one of coupled to and formed integrally with the container such that there is no relative movement there-between. The method includes receiving a medium and cells in a container and oscillating the medium such that the cells traverse along a longitudinal direction of the container. | 2013-03-07 |
| 20130059377 | STEM CELL DEFINED MEDIA FOR XENO-FREE AND FEEDER FREE CONDITIONS AND USES THEREOF - The invention provides a defined low protein culture medium for maintaining cells in an undifferentiated state, the medium comprising: a basal medium, an organic acid from the tricarboxylic acid cycle, nonessential amino acids, a combination of growth factors selected from the group consisting of FGF-2 protein, an IGF-1 protein or insulin, a Transferrin protein, and a TGF beta 1 protein, wherein the medium is essentially feeder-free, essentially xeno-free, essentially free of beta-mercaptoethanol, and essentially free of animal-derived or human-derived proteins. | 2013-03-07 |
| 20130059378 | HUMAN EPIDERMIS-DERIVED MESENCHYMAL STEM CELL-LIKE PLURIPOTENT CELLS AND PREPARATION METHOD THEREOF - The invention discloses a cell culture medium, human epidermis-derived mesenchymal stem cell-like pluripotent cells and a preparation method thereof. The culture medium is formed by adding fetal bovine serum, hbFGF, hSCF, non-essential amino acids, L-glutamine and gentamicin into a DMEM culture medium with the glucose content of 0.5-5 g/L, wherein every 100 mL of the final culture medium contains 10-25% by volume percent of fetal bovine serum, 100-4000 ng of the hbFGF, 10-2000 ng of hSCF, 0.1-2 mL of 100× non-essential amino acids, 0.1-2 mL of PBS solution containing 3% by mass percent of L-glutamine and 1000-8000 U of gentamicin. A human epidermis-derived mesenchymal stem cell-like pluripotent cell strain is prepared by culturing human epidermal cells in the culture medium, digesting with digestive juice, removing undigested human epidermal cells, collecting digested mesenchymal stem cell-like cells and performing in-vitro culture and passage. | 2013-03-07 |
| 20130059379 | METHOD FOR THE GENERATION OF A CIK CELL AND NK CELL POPULATION - The present invention is directed a method for the generation and/or expansion of CIK cell and/or NK cells by culturing peripheral blood cells in the presents of cytokines. The cytokines used in the method comprise interleukin 15 (IL-15) and interleukin 7 (IL-7), possibly in combination with further cytokines like interleukin 2 (IL-2) stem cell factor (SCF) and Fms-related tyrosine kinase 3 ligand (FLT3 ligand). | 2013-03-07 |
| 20130059380 | PRESERVATION SOLUTIONS FOR BIOLOGICS AND METHODS RELATED THERETO - The present invention provides compositions (i.e., preservation solutions) for preserving biologies comprising at least one membrane penetrable sugar, at least one membrane impenetrable sugar, at least one anti-microbial agent, at least one anti-oxidant, adenosine, albumin, a salt, a buffer, and a chelating agent, and to methods of using such preservation solutions to preserve biologies. | 2013-03-07 |
| 20130059381 | SOLUTION FOR NON-PROGRAMMED CELL CRYOPRESERVATION - The invention provides a solution for non-programmed cell cryopreservation, which comprises 1.0-28 w/v % of cell membrane protectant, 1.0-18 w/v % of permeable intracellular protectant, 3.0-28 w/v % of cell sedimentation stabilizer, and the balance of solvent. The cell cryopreservation solution provided by the invention is good for protecting cells. After the cryopreservation solution is added to cells, the cells can be cryopreserved in a refrigerator of −80 DEG C. directly without complicated programmed cryopreservation, thus, the time for cell cryopreservation is shortened greatly and the efficiency of cryopreservation is improved. Therefore, the cryopreservation solution is suitable for cryopreserving a large number of cells. The recovery rate of the cells cryopreserved is high, the growth and differentiation of the recovered cells is normal. The components of the cryopreservation solution are stable, thus the cryopreservation solution can be preserved with good property for a long time. | 2013-03-07 |
| 20130059382 | BIOMEDICAL MATERIALS FOR TISSUE ENGINEERING - In an embodiment of the disclosure, a biomedical material is provided. The biomedical material includes a biocompatible material having a surface and a carrier distributed over the surface of the biocompatible material, wherein both of the biocompatible material and the carrier have no charges, one of them has charges or both of them have charges with different electricity. The biomedical material is utilized for dentistry, orthopedics, wound healing or medical beauty and applied in the repair and regeneration of various soft and hard tissues. | 2013-03-07 |
