08th week of 2010 patent applcation highlights part 48 |
Patent application number | Title | Published |
20100047818 | DIAGNOSIS OF CARCINOMAS - The invention is directed to compositions and methods for the detection of a malignant condition, and relates to the discovery of soluble and cell surface forms of HE4a polypeptides, including HE4a that is overexpressed in ovarian carcinomas. In particular the invention provides a nucleic acid sequence encoding HE4a, and also provides a method of screening for the presence of a malignant condition in a subject by detecting reactivity of an antibody specific for a HE4a polypeptide with a molecule naturally occurring in soluble and/or cell surface form in a sample from such a subject, and by hybridization screening using an HE4a nucleotide sequence, as well as other related advantages. | 2010-02-25 |
20100047819 | MULTIPLEX ASSAY FOR RHEUMATOID ARTHRITIS - Multiplex assays that allow for the detection and quantification of Rheumatoid Factor (RF) and anti-cyclic citrullinated peptide (CCP) antibodies in a single reaction mixture are provided. | 2010-02-25 |
20100047820 | DETECTING METHOD, DETECTING APPARATUS, DETECTION SAMPLE CELL, AND DETECTING KIT - A sample is supplied onto the sensor portion of a sensor chip. An excitation light beam is irradiated to generate an enhanced optical field to be generated on the sensor portion. Fluorescent labels are excited, and the amount of a detection target substance is detected, based on the amount of light which is generated due to excitation of the fluorescent labels. A first electric charge is present on the surface of the sensor portion. A fluorescent substance having fluorescent pigment molecules which are enveloped in a light transmitting material that transmits fluorescence generated by the fluorescent pigment molecules, the surfaces of which are charged with second electric charges opposite the first electric charge on the surface are employed as the fluorescent labels. The fluorescent substance is attracted to the sensor portion by static electric interactions between the two charges. | 2010-02-25 |
20100047821 | CROWN ETHER DERIVATIVES - The invention describes crown ether chelators, including crown ethers having the formula: | 2010-02-25 |
20100047822 | TIMP-4 AS A BIOMARKER FOR THE DIAGNOSIS OF CARDIAC INSUFFICIENCY - The invention relates to the use of the plasma concentration of the polypeptide “tissue inhibitor of metalloproteinase-4” (TIMP-4) as a biomarker for the diagnosis of heart failure. | 2010-02-25 |
20100047823 | Polypeptides - The use of polypeptides capable of binding to PtdIns(3,4)P | 2010-02-25 |
20100047824 | CITRUS FRUIT PEELING PROCESS - An efficient process for enzymatically peeling citrus fruit applies a vacuum to perforated fruit and, while maintaining that vacuum, introduces an enzyme solution to the perforated fruit. The fruit is infused with the enzyme by releasing the vacuum pressure. After incubating the enzyme, the albedo of the fruit is weakened and the citrus peel can be readily removed. The peeled fruit may be divided into sections and the encompassing membrane removed. | 2010-02-25 |
20100047825 | IMMUNOASSAY REAGENTS AND METHODS OF USE THEREOF - The present invention provide reagents and methods of using the reagents, for example, on automated staining devices, that facilitate detection of two or more antigens in a sample simply and efficiently. | 2010-02-25 |
20100047826 | Cell Into Which Protein, Which Can Serve as Polymerization Nucleus of Protein Polymer, or Polymer Thereof Is Introduced, and Method for Production of The Cell - The present invention has the object of providing a cell into which a protein, which can serve as a polymerization nucleus of a protein polymer, or polymer thereof is introduced, and a method for producing the cell. The invention relates to a cell into which a protein, which can serve as a polymerization nucleus of a protein polymer, or a polymer thereof is introduced, a method for producing the cell, and a method of screening for a compound inhibiting an intracellular accumulation of a protein containing fibril structures, wherein the method comprises bringing a candidate substance into contact with the cell. | 2010-02-25 |
20100047827 | NOVEL SPECIFIC CELL BINDERS - The invention describes reagents and methods for specific binders to glycan structures of stem cells. Furthermore the invention is directed to screening of additional binding reagents against specific glycan epitopes on the surfaces of the stem cells. The preferred binders of the glycans structures includes proteins such as enzymes, lectins and antibodies. | 2010-02-25 |
20100047828 | SOLID-PHASE DETECTION OF TERMINAL MONOSACCHARIDES CLEAVED FROM GLYCOSYLATED SUBSTRATES - The present invention relates to a novel method for analysing carbohydrates. The invention is in particular useful in detecting a terminal monosaccharide which may be released from a glycosylated substrate for example using an exoglucosidase. After relase from the glycosylated substrate the terminal monosaccharide may be captured on a solid support, incubated with a boronate detection agent and detected by aid of the boronate detection agent. The methods of the invention are useful for a variety of purposes including sequencing of carbohydrates, wherein exoglycosidases with predetermined specificity are employed for the release. | 2010-02-25 |
20100047829 | ACTIVATED HER3 AS A MARKER FOR PREDICTING THERAPEUTIC EFFICACY - The present invention provides methods for the determination of the activation level of Receptor Tyrosine kinases, e.g. phosporylated HER3, for the selection of patients for disease treatment. Methods are also provided for the evaluation of the biological and pharmacodynamic effects of an active substance and/or its efficacy in disease treatment, utilizing a tissue sample from a test subject, for example tumor material or normal tissue such as skin or hair follicle. Further, methods for the treatment of HER receptor-associated diseases are disclosed. | 2010-02-25 |
20100047830 | COMPOSITIONS AND METHODS FOR DETECTING CANCERS IN A SUBJECT - Disclosed are compositions and methods for detecting oral and gastrointestinal cancers in a subject. Autoantigen p90 was shown to be overexpressed in oral cancer cells, and this protein, as well as its companion autoantigen p62 and antibodies directed to both proteins, can be used as markers for detecting oral digestive and other cancers in a subject at an early stage. | 2010-02-25 |
20100047831 | DIAGNOSIS OF ANTHRAX INFECTION BY DETECTION OF CAPSULAR ANTIGEN IN URINE - The present invention provides methods useful for diagnosis of anthrax by detection of capsular polypeptide in serum, urine, or other biological samples. | 2010-02-25 |
20100047832 | Assessment of Patients with Sepsis to Determine a Requirement for Therapeutic Intervention with an Anti-Inflammatory and/or Anticoagulatory Agent - Methods of selecting patients with sepsis for treatment with an anti-inflammatory and/or anticoagulatory agent are provided. | 2010-02-25 |
20100047833 | DIAGNOSTIC METHOD FOR THE DETERMINATION OF HELICOBACTER PYLORI - The present invention relates to a diagnostic method for the determination of | 2010-02-25 |
20100047834 | METHOD FOR STABILIZING ALPHA-THROMBIN IN THROMBIN-CONTAINING SOLUTION - To provide a method for stabilizing unstable α-thrombin in a thrombin-containing solution, a solution containing stabilized α-thrombin, and a liquid fibrinogen assay reagent containing the solution. The method for stabilizing α-thrombin in a thrombin-containing solution, which includes adjusting the percentage of α-thrombin to 70% or more with respect to the amount of total thrombin in the thrombin-containing solution. | 2010-02-25 |
20100047835 | DIAGNOSIS OF INFECTIONS OR INFLAMMATORY DISEASES OF THE AIRWAYS AND LUNGS ASSOCIATED WITH HEART FAILURE - The invention relates to a method for diagnosis of infections or inflammatory diseases of the airways and lungs with associated heart failure, wherein the marker procalcitonin or a partial sequence thereof is determined in a patient to be examined, in particular for classifying patients according to risk. The invention further relates to a diagnostic device and kit for carrying out the method. | 2010-02-25 |
20100047836 | LIPOLYTIC ENZYME VARIANTS - Molecular dynamics (MD) simulation on the three-dimensional structure of | 2010-02-25 |
20100047837 | METHOD FOR THE EARLY DETECTION OF RENAL INJURY - A method and kit for detecting the immediate or early onset of renal disease and injury, including renal tubular cell injury, utilizing NGAL as an immediate or early on-set biomarker in a sample of blood serum. NGAL is a small secreted polypeptide that is protease resistant and consequently readily detected in the blood serum following renal tubule cell injury. NGAL protein expression is detected predominantly in proximal tubule cells, in a punctuate cytoplasmic distribution reminiscent of a secreted protein. The appearance NGAL in the serum is related to the dose and duration of renal ischemia and nephrotoxemia, and is diagnostic of renal tubule cell injury and renal failure. NGAL detection is also a useful marker for monitoring the nephrotoxic side effects of drugs or other therapeutic agents. | 2010-02-25 |
20100047838 | YEAST GENES THAT AFFECT VIRAL REPLICATION - An antiviral agent comprising an altered MAB1, MAB2, MAB3, or OLE1 gene, gene homologs or related genes is disclosed. In another embodiment, the present invention is a method of creating a virus resistant organism comprising creating a transgenic organism comprising an antiviral agent selected from the group of altered MAB1 genes, MAB2 genes, MAB3 genes or OLE1 genes, homologs of these genes, related genes and combinations of these genes and homologs. | 2010-02-25 |
20100047839 | LATENT FLUORIMETRIC INDICATOR FOR BIOLOGICAL ANALYTES DETERMINATION AND THE PREPARATION METHOD THEREOF - The present invention provides a sensitive fluorimetric indicator for analytes determination in the oxygen-insensitive DT-diaphorase-coupled dehydrogenases assay by omitting NADH, which is generated by reaction in the presence of analytes, which presents to the applicability as a biosensor for future clinical diagnostic. Furthermore, the novel long-wavelength latent fluorimetric indicator is also a user-friendly probe for monitoring DT-diaphorase activity. The fluorescence signal revealed by this process is specific and exhibited in the near red spectrum region. | 2010-02-25 |
20100047840 | Polypeptide markers for the diagnosis of bladder cancer - A method for the diagnosis of bladder cancer (BC) and/or for determining a tumor stage of bladder cancer, comprising the step of determining the presence or absence or amplitude of at least six polypeptide markers in a sample, wherein said polypeptide markers are selected from markers 1 to 836, which are characterized by the values for the molecular masses and migration times (CE time). | 2010-02-25 |
20100047841 | SYNTHESIS OF DESACETOXYTUBULYSIN H AND ANALOGS THEREOF - Compounds of formula I, XVI and XXI possess potent cell growth inhibitory activity. These compounds are described have therapeutic utility, particularly in the treatment of cancer as well as conditions and disorders related to uncontrolled cell growth: | 2010-02-25 |
20100047842 | NOVEL TOXICITY ASSAY BASED ON HUMAN BLASTOCYST-DERIVED STEM CELLS AND PROGENITOR CELLS - An in vitro toxicity assay based on human blastocyst-derived stem cells for the detection of toxicity in the human species is provided, which enables novel detection of in vitro human toxicity for a substance and/or more efficiently detects human toxicity compared to non-human assays. Furthermore, the detection of toxicity for substances is enabled, which is known to display inter-species differences and the toxic effect was not detectable by toxicological tests in mice. | 2010-02-25 |
20100047843 | COMPOSITIONS AND METHODS FOR ENHANCING THE GROWTH OF MOUSE EMBRYONIC STEM CELLS - Compositions and methods are provided which improve the growth rate, self-renewal potential and capacity of germ line transmission of mouse embryonic stem cells. | 2010-02-25 |
20100047844 | DIAGNOSTIC MARKER FOR FABRY DISEASE - The present invention is in the field of Fabry disease and concerns a pathogenic factor allowing diagnosis of Fabry disease. In particular lyso-ceramide trihexosamide (lyso-CTH) has been found to function as a diagnostic marker for Fabry disease. | 2010-02-25 |
20100047845 | METHOD OF REDUCING CURVATURE IN A MENISCUS OF LIQUID MEDIUM - The present application is directed to methods of improving cell culture vessel assays. In one aspect the application is directed to a method of reducing the curvature of the meniscus comprising applying a coating material to the interior wall of the vessel, wherein the coating material provides a receding contact angle of about 90 degrees with aqueous solutions and culture media. In another aspect, the application is directed to a method of labeling cells in a first solution by generating droplets of a second solution containing at least one cell-labelling agent and allowing the droplets of the second solution to contact the surface of the first solution. | 2010-02-25 |
20100047846 | ENDOGENOUS AND NON-ENDOGENOUS VERSIONS OF HUMAN G PROTEIN-COUPLED RECEPTORS - The invention disclosed in this patent document relates to transmembrane receptors, more particularly to a human G protein-coupled receptor for which the endogenous ligand is unknown (“orphan GPCR receptors”), and most particularly to mutated (non-endogenous) versions of the human GPCRs for evidence of constitutive activity. | 2010-02-25 |
20100047847 | Methods for diagnosing ovarian cancer - The present invention provides protein-based biomarkers and biomarker combinations that are useful in qualifying ovarian cancer status in a patient. In particular, the biomarkers of this invention are useful to classify a subject sample as ovarian cancer, ovarian cancer of low malignant potential, benign ovarian disease or other malignant condition. The biomarkers can be detected by SELDI mass spectrometry, immunoassay, or other means. | 2010-02-25 |
20100047848 | Colorimetric determination of somatic cell count in milk - A simple calorimetric in-line quantitative test to measure white blood cell counts in milk samples using a liquid reagent system that simplifies quantitative in-line SCC measurements using a reflectance measuring mode, and a new apparatus, which permits in-line colorimetric analysis. | 2010-02-25 |
20100047849 | DETERMINATION OF TESTOSTERONE BY MASS SPECTROMETRY - Provided are methods for determining the presence or amount of testosterone in a test sample, comprising ionizing all or a portion of the testosterone present in the sample to produce one or more testosterone ions that are detectable in a mass spectrometer. All or a portion of the testosterone present in the sample is ionized to produce one or more testosterone ions, which may be isolated and fragmented to produce precursor ions. A separately detectable internal testosterone standard can be provided in the sample. In a preferred embodiment, the reference is 2,2,4,6,6-d | 2010-02-25 |
20100047850 | METHOD AND DEVICE FOR RAPID DETECTION OF MICROORGANISMS BY CHANGING THE SHAPE OF MICRO-COLONIES IN MICRO-CHANNELS - This invention describes a method of rapid detection of micro-colonies of microorganisms by changing their shape from a regular semi-sphere to a long and thin cylinder. Cells are trapped by filtration in long (diameter/length=1/10-1/100), cylindrical, parallel, micro-channels that are open from both sides, and attached to a filter from one side. A micro-channel plate houses a multiplicity of micro-channels (diameter of each channel=1-20 μm, and length 100-1000 μm). The micro-channel plate with cells trapped on the surface of the filter is attached to a nutrient media agar block. Cells produce micro-colonies of a long and thin shape according the shape of the micro-channel. The growth of microorganisms in the micro-channels permits a change in the number of cells to accomplish light absorbance. Fewer cells need a shorter time to reproduce. Thus detection and counting of cells can be accomplished in a rapid fashion. The light absorbance can be enhanced by additional coloration of micro-colonies by cell dyes or artificial chromogenic or fluorogenic substrates. | 2010-02-25 |
20100047851 | Methods and compositions for the detection of microbial contaminants - The invention provides methods and compositions for the detection and/or quantification of a microbial contaminant, for example, a bacterial endotoxin or a glucan, in a sample. In particular, the invention provides a test cartridge useful in the practice of hemocyte lysate-based assays for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides methods of making and using such cartridges. In addition, the invention provides a rapid, sensitive, multi-step kinetic hemocyte lysate-based assay for the detection and/or quantification of a microbial contaminant in a sample. In addition, the invention provides a glucan-specific lysate that can be used in a variety of assay formats, including, for example, a test cartridge, optionally configured to perform a kinetic assay. | 2010-02-25 |
20100047852 | SELECTIVE CULTURE MEDIUM - The present invention discloses a selective culture medium for detecting MRSA and selective drug or multidrug resistant microorganisms where the expression of said (multi)drug resistance is by virtue of the production by said microorganisms of β-lactamase enzymes. The medium comprises: (i) a broad spectrum antibiotic capable of selectively inhibiting growth/replication of (a) organisms that do not produce β-lactamase enzymes, (b) organisms that while producing β-lactamase do not exhibit an ESBL or AmpC phenotype and (c) Methicillin-susceptible | 2010-02-25 |
20100047853 | Ex Vivo Culture, Proliferation and Expansion of Intestinal Epithelium - Methods are provided for long term culture of mammalian intestinal cells. Cultures are initiated with fragments of mammalian intestinal tissue, which are then maintained embedded in a gel substrate that provides an air-liquid interface. Intestinal epithelium in cultures of the invention can be continuously grown for extended periods of time. Mammalian intestinal cells cultured by the methods of the invention recapitulate features of intestinal growth in vivo. | 2010-02-25 |
20100047854 | METHOD FOR DETECTION OF HUMAN PRECURSOR T CELL AND PRECURSOR B CELL - A method for detection of a precursor T cell or precursor B cell, a method for evaluation of the property of a hematopoietic precursor cell in a source for transplantation, and a kit for use in the evaluation are disclosed. A precursor T cell or precursor B cell can be detected by co-cultivating a stromal cell line with a monocyte. By using the detection method, a precursor T cell or precursor B cell can be quantified and can also evaluate the property of a hematopoietic precursor cell in a source for transplantation. | 2010-02-25 |
20100047855 | METHOD FOR DETERMINING THE QUANTITY OF MICROBIOLOGICAL OBJECTS DURING CULTIVATION THEREOF - A method for determining the quantity of microbiological objects during the cultivation thereof comprises determining a modification of studied microorganism cells' population, measuring the morphological composition thereof by determining the cells' size distribution in a liquid medium according to intensity changes of the light dispersed thereby. The method comprises probing a liquid flow by monochromatic coherent light, recording signals relating to the interaction between the probing radiation and the cells by measuring amplitudes and durations of impulses of the light dispersed by medium particles, plotting functions, according to measurement results, as two-dimensional distributions of normalized values of the amplitudes and durations in the form of statistic parameters of the dispersed light intensity. According to the functions, the size distribution of the cells and decomposition products of the liquid nutrient medium during the cultivation thereof are obtained. The invention can be applied for medical diagnostics and for control of biotechnological processes. | 2010-02-25 |
20100047856 | Bacteria analyzer, bacteria analyzing method and computer program product - The present invention is to present a bacteria analyzer comprising: a detector comprising: a light source for irradiating light on a measurement sample prepared from a specimen and a reagent; and a light receiving unit for receiving light generated by irradiating the light on the measurement sample from the light source; a scattergram data acquirer for acquiring scattergram data for generating a scattergram having information related to size of the bacteria contained in the specimen and fluorescence information generated by the bacteria as parameters; a bacteria number acquirer for acquiring number of bacteria contained in a plurality of regions on the scattergram for each region; and a form determiner for determining a form of the bacteria contained in the specimen. | 2010-02-25 |
20100047857 | MULTIPLE ANALYTE IMMUNOASSAY - Methods for measuring the amount of two or more analytes of interest in a fluid sample, and kits useful in the methods, are disclosed. The methods involve determining a ratio of a detected amount of a single analyte of interest, to the sum of a detected amount of each of the analytes of interest plus a detected amount of a control, wherein the amount of each analyte of interest is directly or inversely related to the ratio for each analyte of interest. | 2010-02-25 |
20100047858 | HIGH THROUGHPUT SYSTEM FOR CFU ASSAY BY THE USE OF HIGH RESOLUTION DIGITAL IMAGING, DIFFERENTIAL STAINING AND AUTOMATED LABORATORY SYSTEM - Disclosed herein is a high throughput system for the objective, standardized determination of colony forming units in populations of hematopoietic cells and indication of successful engraftment. Further disclosed is a laboratory information management system that provides electronic storage of images and data associated with cord blood units. | 2010-02-25 |
20100047859 | SILICA-BASED FLUORESCENT NANOPARTICLES - A composition including the reaction product of: an organic silane of Formula SiR | 2010-02-25 |
20100047860 | DEVICE FOR STORING SPECIMEN SLICE AND INSTRUMENT FOR MICROSCOPIC OBSERVATION PROVIDED WITH THE SAME - [Problems] It is intended to provide a storing device which is convenient in preserving a slice of a pathological tissue or the like over a long time and by which a required number of slices can be quickly provided without waste. It is also intended to provide an instrument for microscopic observation which is equipped with this storing device and a transparent plate having an adhesive layer formed thereon. | 2010-02-25 |
20100047861 | PROCESS FOR PRODUCING USEFUL SUBSTANCE - The present invention provides processes of producing useful substances, which include the steps of culturing in a medium microorganisms that lack from their chromosomal DNA all or part of a gene encoding a protein having the amino acid sequence shown in SEQ ID NO: 1 or a gene encoding a protein that is 80% or more homologous to the amino acid sequence shown in SEQ ID NO: 1 so as to produce and accumulate the useful substances in the culture, and recovering the useful substances from the culture. | 2010-02-25 |
20100047862 | Novel DNA Polymerase - This invention provides a novel DNA polymerase obtained from | 2010-02-25 |
20100047863 | GROUP OF NOVEL ENANTIOSLEECTIVE MICROBIAL NITRILE HYDRATASES WITH BROAD SUBSTRATE SPECIFICITY - The present invention provides a polynucleotide or a pair of polynucleotides encoding an enzyme having nitrile hydratase (NHase) [E.C. 4.2.1.84] activity. Furthermore, a vector and a host comprising the disclosed polynucleotide or pair of polynucleotides and methods for the production of the same are provided. Moreover, the invention relates to a pair of polypeptides or a fusion protein having NHase activity, an antibody specifically binding to the pair of polypeptides or fusion protein, a primer or probe, which specifically hybridizes under stringent conditions to the disclosed polynucleotide or either one of the pair of polynucleotides, a composition comprising the polynucleotide or pair of polynucleotides, the pair of polypeptides or fusion protein, the antibody and/or one or more primers or probes of the invention and a method for the production of amides comprising the enantioselective conversion of nitrites. | 2010-02-25 |
20100047864 | PROCESS FOR THE PRODUCTION OF RECOMBINANT PROTEINS USING CARNIVOROUS PLANTS - The present application relates to the provision of process for producing at least one protein, comprising the cultivation of a carnivorous plant, characterized in that said plant has been genetically modified to express said protein or proteins, and said protein or proteins are collected from the digestive secretions of said carnivorous plant traps, in particular glue, pitcher, trumpet or bladder traps. The proteins of interest are functional, despite the existence of digestive enzymes. | 2010-02-25 |
20100047865 | VECTOR FOR EXPRESSING NC PROTEIN OF HIV AND METHOD FOR PRODUCING NC PROTEIN USING THE SAME - The present invention relates to a vector for expressing an NC protein of HIV and a method for producing an NC protein using the same. More particularly, the present invention relates to a vector for expressing an NC protein of HIV, in which an intron sequence and an mRNA stability element in the downstream of NC gene are sequentially linked, and a method for producing an NC protein using the same. The vector for expressing an NC protein of HIV of the present invention, in which an intron sequence and an mRNA stability element in the downstream of NC gene are sequentially linked, can express a wild type NC protein in animal cells, and has an effect of improving the expression efficiency, as compared to a known art. | 2010-02-25 |
20100047866 | Heat-Stable Carbonic Anhydrases and Their Use - The present invention relates to use of heat-stable carbonic anhydrase in CO | 2010-02-25 |
20100047867 | IL-1 ZETA, IL-1 ZETA SPLICE VARIANTS AND XREC2 DNAS AND POLYPEPTIDES - The invention is directed to novel, purified and isolated IL-1 zeta and Xrec2 polypeptides and fragments thereof, the nucleic acids encoding such polypeptides, processes for production of recombinant forms of such polypeptides, antibodies generated against these polypeptides, fragmented peptides derived from these polypeptides, and uses thereof. | 2010-02-25 |
20100047868 | Tumour Suppressor Protein - We describe a polypeptide which binds and modulates the activity of a tumour suppressor polypeptide, for example p53; a nucleic acid molecule encoding said protein and screening methods which modulate the binding activity of said polypeptide for its target polypeptide(s). | 2010-02-25 |
20100047869 | Novel Thermophilic Endo-Glucanase and Uses Thereof - A novel thermophilic endo-glucanase, nucleic acid encoding the endo-glucase, and uses thereof in converting lignocellulosic material to fermentable sugars. | 2010-02-25 |
20100047870 | LOW CELL DENSITY FERMENTATION PROCESS FOR THE PRODUCTION OF HETEROLOGOUS RECOMBINANT PROTEINS IN MICROORGANISMS - A low cell density fermentation process for the production of heterologous proteins in microorganisms. The cell culture obtained by cultivating host microorganisms transformed with a vector carrying genetic material for the said proteins and an inducible promoter under batch fermentation conditions is fed with a feed medium after an OD | 2010-02-25 |
20100047871 | FUSION PROTEINS OF HIV REGULATORY/ACCESSORY PROTEINS - The invention relates to fusion proteins comprising the amino acid sequence of at least three HIV proteins selected from Vif, Vpr, Vpu, Rev, and Tat or derivatives of the amino acid sequence of one or more of said proteins, wherein the fusion protein is not processed to individual HIV proteins having the natural N and C termini. The invention further concerns nucleic acids encoding said proteins, vectors comprising said nucleic acids, and methods for producing said proteins. The fusion protein, nucleic acids and vectors are usable as vaccines for the at least partial prophylaxis against HIV infections. | 2010-02-25 |
20100047872 | PROCESS FOR PRODUCING USEFUL SUBSTANCE - The present invention provides a process for producing a useful substance by use of a microorganism that lacks in their chromosomal DNA all or part of a gene encoding a protein having the amino acid sequence shown in SEQ ID NO: 1 or a gene encoding a protein having 80% or more, preferably 90% or more, more preferably 95% or more, even more preferably 97% or more, still more preferably 98% or more, and yet more preferably 99% or more homology with the amino acid sequence shown in SEQ ID NO: 1. | 2010-02-25 |
20100047873 | PROCESS FOR THE PRODUCTION OF DAPTOMYCIN - The present invention relates to an improved process for the production of Daptomycin by fermentation with | 2010-02-25 |
20100047874 | PURINE-DERIVED SUBSTANCE-PRODUCING BACTERIUM AND A METHOD FOR PRODUCING A PURINE-DERIVED SUBSTANCE - A purine-derived substance is produced by culturing a bacterium belonging to the genus | 2010-02-25 |
20100047875 | Making nucleic acid sequences in parallel and use - The present invention relates generally to the fields of genomics, synthetic biology and genetic engineering. More particularly, the present invention concerns the methods that enable parallel multiplex ligation and amplification on surface for making assemblies of nucleic acids of various biological applications and for analysis of biological samples such as DNA, RNA, and proteins. | 2010-02-25 |
20100047876 | HIERARCHICAL ASSEMBLY OF POLYNUCLEOTIDES - Methods, compositions and apparatuses for hierarchical assembly of oligonucleotide sequences are provided. | 2010-02-25 |
20100047877 | Novel phospholipases and uses thereof - The invention relates to a newly identified polynucleotide sequence comprising a gene that encodes a novel phospholipase isolated from | 2010-02-25 |
20100047878 | METHOD OF PRODUCING L-AMINO ACID - An L-amino acid is produced by culturing a microorganism belonging to the family Enterobacteriaceae having an L-amino acid-producing ability and modified so that glycerol dehydrogenase and dihydroxyacetone kinase activities are increased, in a medium containing glycerol as a carbon source to produce and accumulate an L-amino acid in the medium or cells, and collecting the L-amino acid from the medium or the cells. | 2010-02-25 |
20100047879 | PRODUCING METHIONINE WITHOUT N-ACYL-METHIONINE - The present invention relates to a method for the production of methionine or its derivatives by culturing a microorganism in an appropriate culture medium comprising a source of carbon and a source of sulphur. The microorganism and/or the culture medium and/or the process parameters were modified in a way that the accumulation of the by-product N-acyl-methionine (NAM) is reduced. The isolation of methionine or its derivatives from the fermentation medium is also claimed. | 2010-02-25 |
20100047880 | PRODUCTION OF N-ACYLATED SULPHUR-CONTAINING AMINO ACIDS WITH MICROORGANISMS HAVING ENHANCED N-ACYLTRANSFERASE ENZYMATIC ACTIVITY - The present invention claims an isolated polypeptide having L-amino-acid-N-acyl transferase enzymatic activity and a modified microorganism in which this enzyme is overexpressed. Substrates of said enzyme include mainly methionine and their derivatives or analogs. Overexpression in sulphur-containing amino acid producing microorganisms permits the production of large amounts of N-acylated sulphur-containing amino acids. The isolation of the N-acylated sulphur-containing amino acids from the fermentation medium is also claimed. | 2010-02-25 |
20100047881 | Microorganisms with Deregulated Vitamin B12 System - The present invention relates to microorganisms with improved efficiency of vitamin B12 utilization. | 2010-02-25 |
20100047882 | METHOD OF RECOVERING L-THREONINE FROM L-THREONINE FERMENTATION BROTH USING NONSOLVENT - Provided are a method of recovering L-threonine from the fermentation broth of an L-threonine producing microorganism, comprising: separating microbial bodies from the L-threonine containing fermentation broth obtained by culturing an L-threonine producing microorganism and filtering the separated fermentation broth to obtain a filtrate; concentrating the filtrate; and reacting the concentrated filtrate with a nonsolvent to obtain crystalline L-threonine, crystalline L-threonine recovered by the method, and a feed additive containing the crystalline L-threonine recovered by the method. | 2010-02-25 |
20100047883 | PROCESS FOR PRODUCING USEFUL SUBSTANCE USING IMMOBILIZED ENZYME - The present invention relates to a process for producing a useful substance, the process including feeding a liquid mixture formed of two liquid phases into a fixed bed-type reaction column packed with an immobilized enzyme, and allowing the liquid mixture to flow in the same direction in a co-current manner to perform a reaction, wherein the packing thickness of the immobilized enzyme per stage of the fixed bed-type reaction column having an equivalent circular diameter of 35 mmφ or larger is 10 to 200 mm. | 2010-02-25 |
20100047884 | Method for Producing Biodiesel - The invention relates to the utilisation of fatty materials with substantial free fatty acid content in the production of biodiesel by the use of microbial enzymes that are effective in a solvent-free process for the production of esters of fatty acids and C | 2010-02-25 |
20100047885 | Pantothenate Kinase Overexpression and Pantothenic Acid Supplementation in Actinomycetes - The invention relates to the synthesis of polyketides in Actinomycetes by overexpression of pantothenate kinase and supplementation with pantothenic acid. This results in increasing in vivo CoA production and thereby drives increased production of secondary metabolites, such as polyketides. | 2010-02-25 |
20100047886 | METHOD OF CONVERSION OF SYNGAS USING MICROORGANISM ON HYDROPHILIC MEMBRANE - A stable system for producing liquid products such as ethanol, butanol and other chemicals from syngas components contacts CO or a mixture of CO | 2010-02-25 |
20100047887 | METHOD FOR PREPARING HYDROXYTYROSOL - The present invention relates to newly identified microorganisms capable of direct production of hydroxytyrosol (hereinafter also referred to as Hy-T) from tyrosol. The invention also relates to polynucleotide sequences comprising genes that encode proteins which are involved in the synthesis of Hy-T from tyrosol. Also included are methods/processes of using the polynucleotides and modified polynucleotide sequences to transform host microorganisms. The invention also relates to genetically engineered microorganisms and their use for the conversion of tyrosol into Hy-T. | 2010-02-25 |
20100047888 | Method and apparatus for bio-fuel seeding - A method and apparatus is provided for microbial seeding and amendment of traditional alternative fuels production systems and processes using immobilized microbe bioreactors. The system addition utilizes attachment of yeast or other microbial consortia to a substrate to enhance alternative fuels production in fermentation processes. The system allows for the maintenance of a constant concentrated microbial population, thus enhancing alternative fuels production by stabilizing microbial populations. Desired aerobic and anaerobic conditions are maintained using a microbubble aeration device coupled to the Immobilized Microbe Bioreactor (IMBR) seeding reactors. Generation of the microbial populations for seeding requires control of aerobic and anaerobic conditions to ensure growth of a microbial population acclimated to elevated alternative fuels concentrations. | 2010-02-25 |
20100047889 | Method and apparatus for continuous flow bio-fuel production - A continuous flow system for production of bio-fuels using microbial cultures is provided. The present invention does not utilize batch type production, but follows a continuous flow protocol that eliminates much downtime inherent in conventional bio-fuel production systems while greatly reducing space and equipment requirements. Production is enhanced via controlled program of aeration for microbial growth and anaerobic conditions to ensure fermentation efficiency. As the system becomes more tolerant of alcohol content, efficiency increases. Feedstocks include, but are not limited to, material normally discarded from food production facilities including drink syrups, juices or waste water from corn or sugar processing plants. | 2010-02-25 |
20100047890 | METHODS AND COMPOSITIONS FOR GENERATING SPORULATION DEFICIENT BACTERIA - The present invention relates to methods and compositions for engineering sporulating bacterial cells, particularly a cell of the class | 2010-02-25 |
20100047891 | METHOD OF HEAT-STABILIZING alpha-GLUCAN PHOSPHORYLASE (GP) - An α-glucan phosphorylase having improved thermostability, which obtained by modifying natural α-glucan phosphorylase, and a method for producing this α-glucan phosphorylase having improved thermostability are provided. The natural α-glucan phosphorylase is derived from a plant, this α-glucan phosphorylase having improved thermostability has an amino acid residue which is different from that of the natural α-glucan phosphorylase in at least one position selected from the group consisting of a position corresponding to position 4 in a motif sequence 1L or 1H, a position corresponding to position 4 in a motif sequence 2, and a position corresponding to position 7 in a motif sequence 3L or 3H, and wherein the enzyme activity of α-glucan phosphorylase having improved thermostability at 37° C., after heating in a 20 mM citrate buffer (pH 6.7) at 60° C. for 10 minutes, is 20% or more of the enzyme activity of the α-glucan phosphorylase having improved thermostability at 37° C., before heating. | 2010-02-25 |
20100047892 | METHOD FOR MODIFYING CELLS - The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by changing sialylation and/or fucosylation levels of the cells. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered. The control methods are preferably mass spectrometric methods. | 2010-02-25 |
20100047893 | TYPE III T. BRUCEI ARGININE METHYLTRANSFERASE - The invention relates to enzymes in | 2010-02-25 |
20100047894 | Use of Matrix Metalloproteinases, Mutated and Not Mutated, for the Preparation of Pharmaceutical Compositions, and Mutated Metalloproteinases with Increased Stability - The use of matrix metalloproteinases, mutated and not mutated, for the preparation of pharmaceutical compositions useful in the treatment of pathologies associated with an accumulation of matrix bio-polymers and/or an excess of TIMPs (Tissue Inhihitors MetalloProteinases) is described; mutated matrix metalloproteinases, in which at least an aminoacid residue in a definite position in the protein, has been mutated into a hydrophilic and/or charged aminoacidic residue, obtaining an increased stability toward autoproteolysis are also described. | 2010-02-25 |
20100047895 | Methods for purification of bacterial cells and cell components - The present invention relates to a method for the selective purification of bacterial cells and/or cell components, whereby the purification is performed by means of a solid support. | 2010-02-25 |
20100047896 | Nucleic acid construct containing full length genome of human hepatitis C virus, recombinant full length virus genome-replicating cells having the nucleic acid construct transferred thereinto and method of producing hepatitis C virus particle - The present invention provides a method for replicating efficiently an RNA containing fulllength HCV genomic sequence and a method for producing HCV virus particles containing fulllength HCV replicon RNA or fulllength HCV genomic RNA by using a cell culture system. Further, the present invention relates to a method for producing hepatitis C virus particles which comprises culturing a cell, into which a replicon RNA comprising a nucleotide sequence comprising a fulllength genomic RNA sequence of hepatitis C virus of the genotype 2a, at least one selectable marker gene and/or at least one reporter gene and at least one IRES sequence or the fulllength genomic RNA of hepatitis C virus of the genotype 2a is introduced, and generating virus particles in the culture medium. Still further the present invention relates also to a hepatitis C vaccine and an antibody against hepatitis C virus particles. | 2010-02-25 |
20100047897 | Process for producing modified reconstituted sendai viral envelope specific for drug and/or gene delivery to liver cells - A process for producing a targeted gene and/or drug delivery system for liver cells comprising the steps of: i. chemical reduction of Sendai virus for reduction of HN protein; ii. subjecting the reduced virus to the step of dialysis for removal of the reducing agent; iii. solubilizing the reduced virus with a detergent to obtain a solution; iv. centrifuging the solution to separate the insolubles consisting of reduced HN protein and core of said virus; v. adding histidylated lipid to the supernatant; vi. adding the drug or gene after addition of the lipid; vii. removing the detergent from the envelope and then subjecting it to the step of centrifugation to obtain His lipid F-virosomes with entrapped drug or DNA. | 2010-02-25 |
20100047898 | Mixing pipette - A pipette includes a tubular body having an internal passage, a first end and a second end. The tubular body is deformed inward to create a narrow constriction or slit within the internal passage of the pipette. When vacuum is applied to the pipette and a solution is drawn into the pipette, the slit increases the velocity of the solution while gently deforming and separating clumps of matter (e.g., colonies of bacteria) contained in the solution. As the deformed bacterial colony passes through the slit, the bacteria solution is mixed due to turbulent flow within the pipette created by the increase in velocity. Bacteria colony size is further reduced by alternating the pipette flow direction to cycle the bacteria through the slit two or more times. A sloped entrance to the slit also prevents the oily bacteria from clogging the slit during cycle reversals. | 2010-02-25 |
20100047899 | PROCESS FOR CELLULAR PRODUCTION OF BIOLOGICALLY ACTIVE COMPOUNDS - A process of increasing the cellular production of biologically active compounds is provided. The process is particularly useful for increasing antibiotic production by bacterial cells. The process includes the step of inhibiting the activity of methylmalonyl-CoA mutase. | 2010-02-25 |
20100047900 | Novel l-Lysine-inducible promoter - Disclosed is a novel L-lysine-inducible promoter nucleic acid molecule. Also disclosed are a vector containing the nucleic acid molecule, a host cell transformed with the vector, and a method of inducing expression of a target gene using the L-lysine-inducible promoter nucleic acid molecule. | 2010-02-25 |
20100047901 | SYSTEM AND METHOD FOR ELECTRONIC SENSING OF BIOMOLECULES - A nanoelectronic device is combined with a cellular membrane component to provide a sensor for biomolecules or to provide information about the structure of the membrane. The nanoelectronic device may comprise a network of randomly-oriented nanotubes, or other nanostructure, arranged on a substrate with adjacent electrodes so as to operate as a field-effect transistor sensor or as a capacitive sensor. A cellular membrane is disposed over the nanostructure element. | 2010-02-25 |
20100047902 | Transfer device for culture vessel, culture device and holder for culture vessel - A transfer device for a culture vessel for transferring a culture vessel for culturing a cell, a culture device including the transfer device for a culture vessel, and a holder for a culture vessel for holding a culture vessel, in which the transfer device for a culture vessel includes a transferring unit transferring a culture vessel for culturing a cell, an inputting unit inputting a kind of the culture vessel, a speed setting unit setting a transfer speed of the culture vessel based on a kind of the culture vessel inputted to the inputting unit, and a controlling unit controlling a transfer speed of the transferring unit to be the transfer speed set by the speed setting unit. | 2010-02-25 |
20100047903 | METHODS OF PROCESSING COMPOSITIONS CONTAINING MICROPARTICLES - Methods for processing microparticles involve providing a composition comprising a plurality of solid microparticles and at least one non-volatile material, providing a non-solvent, and exposing the composition to the non-solvent to remove at least a portion of the non-volatile material from the composition while retaining at least the microparticles. | 2010-02-25 |
20100047904 | MATERIALS, METHODS AND SYSTEMS FOR PURIFICATION AND/OR SEPARATION - Materials, methods and systems are provided for the purification, filtration and/or separation of certain molecules such as certain size biomolecules. Certain embodiments relate to supports containing at least one polymethacrylate polymer engineered to have certain pore diameters and other properties, and which can be functionally adapted to for certain purifications, filtrations and/or separations. | 2010-02-25 |
20100047905 | SIMULATED BLOOD COMPONENTS AND METHODS - A control and a cellular fraction for use therein and methods for making the same, including a first cellular component including a plurality of a first group of processed animal red blood cells other than human blood cells; a second cellular component including a plurality of a second group of processed animal red blood cells other than human blood cells; a third cellular component including a plurality of a third group of processed animal red blood cells other than human blood cells; wherein one or more of the first second and third cellular components include a nucleus; and the control simulates erythroblasts of a human blood sample on an automated blood analyzer that analyzes blood cells using impedance, optical measurements or both. | 2010-02-25 |
20100047906 | GERM LINEAGE DERIVED FEEDER CELLS AND METHODS THEREOF - The present disclosure relates to human germ layer derived feeder cells (GLDF cells) and method of generation thereof. Further, it relates to a method for culturing and propagating human embryonic stem cells (hESCs) in a substantially undifferentiated state for several passages on the human GLDF cells. In particular, the present disclosure relates to human GLDF cells which are capable of supporting proliferation of hESCs in a substantially undifferentiated and pluripotent state for several passages. | 2010-02-25 |
20100047907 | METHODS OF TRANSPORTING EPITHELIAL CELL MONOLAYERS - Described herein are tissue culture plates with permeable tissue culture plate inserts therein, which provides the tissue culture plates with apical chamber and a basolateral chambers, wherein cells are deposited on the permeable tissue culture inserts and essentially all of tissue culture medium has been removed from the apical chambers of the tissue culture plates and the basolateral chambers of the tissue culture plates contain a solidifiable form of cell culture medium. Also described are cells that can be deposited and grown on the described tissue culture inserts, methods for transporting a tissue culture plate with a permeable tissue culture plate insert therein on which cells are deposited. Also described is a kit for transporting the tissue culture plates described above, and corresponding methods of use. | 2010-02-25 |
20100047908 | MONOCYTE-DERIVED STEM CELLS - Methods for generating multipotent stem cells from adult peripheral blood monocytes are provided. Monocytes may be de-differentiated into monocyte-derived stem cells (MDSCs) by contacting the monocyte with the de-differentation factors, leukocyte inhibitory factor, macrophage colony-stimulating factor, or a combination thereof. The MDSCs may be differentiated into many different types of cells upon contact with the appropriate differentiation factors. Also provided are compositions comprising the MDSCs or differentiated cells derived from the MDSCs. | 2010-02-25 |
20100047909 | NUCLEIC ACID COMPOUNDS FOR INHIBITING VEGF FAMILY GENE EXPRESSION AND USES THEREOF - The present disclosure provides meroduplex ribonucleic acid molecules (mdRNA) capable of decreasing or silencing one or more VEGF family gene expression. An mdRNA of this disclosure comprises at least three strands that combine to form at least two non-overlapping double-stranded regions separated by a nick or gap wherein one strand is complementary to one or more VEGF family mRNA. In addition, the meroduplex may have at least one uridine substituted with a 5-methyluridine and optionally other modifications or combinations thereof. Also provided are methods of decreasing expression of one or more VEGF family gene in a cell or in a subject to treat one or more VEGF family-related disease. | 2010-02-25 |
20100047910 | SUPRAMOLECULAR COMPLEXES AS PHOTOACTIVATED DNA CLEAVAGE AGENTS - The invention provides supramolecular metal complexes as DNA cleaving agents. In the complexes, charge is transferred from one light absorbing metal (e.g. Ru or Os) to an electron accepting metal (e.g. Rh) via a bridging π-acceptor ligand. A bioactive metal-to-metal charge transfer state capable of cleaving DNA is thus generated. The complexes function when irradiated with low energy visible light with or without molecular oxygen. | 2010-02-25 |
20100047911 | COMPOSITIONS AND METHODS FOR ISOLATION, PROPAGATION, AND DIFFERENTIATION OF HUMAN STEM CELLS AND USES THEREOF - The invention is directed to the field of human stem cells and includes methods and compositions for isolating, propagating, and differentiating human stem cells. The invention provides therapeutic uses of the methods and compositions, including autologous transplantation of treated cells into humans for treatment of Parkinson's and other neuronal disorders. | 2010-02-25 |
20100047912 | APPARATUS, METHOD AND SYSTEM FOR CREATING, COLLECTING AND INDEXING SEED PORTIONS FROM INDIVIDUAL SEED - A method, apparatus, and system for coating, singulating, aligning, ablating, and indexing a number of seeds for testing is described. The apparatus has a carrier designed to hold seeds in a predetermined alignment for ablating a seed sample from a seed portion, the seed portions are indexed to the seed samples. The carrier is then located on the apparatus which delivers the seed samples from the carrier to a collector for laboratory testing. | 2010-02-25 |
20100047913 | Colloidal Gold Single Reagent Quantitative Protein Assay - The invention provides methods for determining the concentration of polypeptides. The methods generally comprise labeling a polypeptide with a metal label such as colloidal gold and quantifiably detecting the polypeptide-metal complex; the methods are improvements over other quantification assays that use colloidal gold. The invention further provides kits for practicing the methods. | 2010-02-25 |
20100047914 | AGENT DETECTION AND/OR QUANTITATION IN A BIOLOGICAL FLUID - A method of assessing retinal disease in an eye of a patient by rapid, point of care, quantitative detection of cytokine levels is provided. | 2010-02-25 |
20100047915 | IDENTIFYING PATIENTS AT RISK FOR LIFE THREATENING ARRHYTHMIAS - The invention is a method for identifying proteins associated with sudden cardiac death (SCD) and for assessing a patient's risk of SCD by determining the amount of one or more SCD-associated proteins in the patient. Typically, the patient submits a sample, such as a blood sample, which is tested for one or more SCD-associated proteins. Based upon the results of the tests, the patient's risk of SCD may be assessed. | 2010-02-25 |
20100047916 | DETECTION OF MATERIALS VIA NITROGEN OXIDE - Methods and devices for detecting the presence of a NO forming material (e.g., a material that can form, or is, a nitrogen monoxide molecule) are disclosed based on detection of fluorescence exhibited by NO molecules in a first vibrationally excited state of a ground electronic state. Such excited NO molecules can be formed, for example, when small amounts of explosives are photodissociated. By inducing fluorescence of the material, a distinct signature of the explosive can be detected. Such techniques can be performed quickly and with a significant standoff distance, which can add to the invention's utility. In another aspection of the invention, methods and apparatus for generating electromagnetic radiation are disclosed. Such methods and apparatus can be used in conjunction with any detection method disclosed herein. | 2010-02-25 |
20100047917 | METHOD FOR ASSAYING SULFUR AND APPARATUS THEREFOR - The present invention relates to a sulfur assaying method comprising converting carbon monoxide in a sample gas generated by combustion of the sample to nitrogen dioxide by a pretreatment, and then measuring the intensity of fluorescence of sulfur dioxide in the sample gas, the pretreatment comprising irradiating the sample gas with light from a low pressure mercury lamp, and also relates to a sulfur assaying apparatus comprising a combustor ( | 2010-02-25 |