07th week of 2015 patent applcation highlights part 45 |
Patent application number | Title | Published |
20150044684 | Method for Preparing Soil Microorganisms and Use Thereof - An object of the present invention is to prepare individual microorganisms or a microflora composition derived from land or seafloor soil in which contamination of land or seafloor soil-derived substances is reduced. The present invention provides [1] a method for preparing, from a land or a seafloor solid samples, microorganisms containing a reduced level of land or seafloor soil-derived substances that inhibit nucleic acid analysis, which comprises (1) the step of disposing a suspension of a land or seafloor soil sample containing microorganisms and land or seafloor soil-derived substance that inhibit nucleic acid analysis as an electrolyte on a surface of a substrate at least a part of which constitutes an electrode, and applying a constant potential to the electrode to attach at least a part of the microorganisms to the surface of the substrate, and (2) the step of applying a high-frequency wave potential to the electrode to detach the microorganisms attached to the surface of the substrate in a viable state, and wherein the constant potential applied in the step (1) is greater than −0.5 V but not greater than −0.2 V (vs. Ag/AgCl), or greater than +0.2 V but not greater than +0.4 V (vs. Ag/AgCl), and the high-frequency wave potential applied in the step (2) has a frequency in the range of from 1 kHz to 20 MHz, and a potential range of ±0.1 V (vs. Ag/AgCl) or smaller. | 2015-02-12 |
20150044685 | METHODS FOR ASSESSING RNA QUALITY - The present description refers to methods and kits for the assessment of RNA quality in a sample. Stable RNA is used as a reference for the assessment of RNA quality, wherein the stable RNA has low susceptibility to nuclease degradation. | 2015-02-12 |
20150044686 | Systems and Methods for Containing Biological Samples - An article for holding a plurality of biological samples includes a substrate a substrate comprising a first surface and an opposing second surface and a plurality of reaction sites in the substrate. Each of the reaction sites extends from an opening in the first surface to an opening in the second surface. The reaction sites comprise a hexagonal shape and are configured to provide sufficient surface tension by capillary action to hold respective biological samples. The reaction sites have a density over at least a portion of the surfaces that is at least 170 holes per square millimeter. At least one of the surfaces may have a surface roughness characterized by an arithmetic average roughness (Ra) that is less than or equal to 5 nanometers. | 2015-02-12 |
20150044687 | METHODS OF LOWERING THE ERROR RATE OF MASSIVELY PARALLEL DNA SEQUENCING USING DUPLEX CONSENSUS SEQUENCING - Next Generation DNA sequencing promises to revolutionize clinical medicine and basic research. However, while this technology has the capacity to generate hundreds of billions of nucleotides of DNA sequence in a single experiment, the error rate of approximately 1% results in hundreds of millions of sequencing mistakes. These scattered errors can be tolerated in some applications but become extremely problematic when “deep sequencing” genetically heterogeneous mixtures, such as tumors or mixed microbial populations. To overcome limitations in sequencing accuracy, a method Duplex Consensus Sequencing (DCS) is provided. This approach greatly reduces errors by independently tagging and sequencing each of the two strands of a DNA duplex. As the two strands are complementary, true mutations are found at the same position in both strands. In contrast, PCR or sequencing errors will result in errors in only one strand. | 2015-02-12 |
20150044688 | Method and Device for Targeted Process Control in a Microfluidic Processor Having Integrated Active Elements - The invention relates to a microfluidic micromechanical system having integrated active elements ( | 2015-02-12 |
20150044689 | Diagnostic Test for Infectious Diseases in Cattle - The invention relates to an isolated or recombinant protein from the shark | 2015-02-12 |
20150044690 | FRET MEASUREMENT DEVICE AND FRET MEASUREMENT METHOD - FRET measurement uses a FRET probe that includes a probe element X containing a donor fluorescent substance and a probe element Y containing an acceptor fluorescent substance and enables FRET to occur when the probe element X and the probe element Y approach to each other or bind together. The modulation frequency of laser light with which the FRET probe is irradiated is adjusted to an optimum modulation frequency that maximizes a difference between the phase difference of donor fluorescence emitted from the donor fluorescent substance with respect to intensity modulation of the laser light at the time when FRET occurs and the phase difference of donor fluorescence emitted from the donor fluorescent substance with respect to intensity modulation of the laser light at the time when FRET does not occur. | 2015-02-12 |
20150044691 | BLOOD MARKER FOR RENAL CANCER - The present invention provides a blood marker for renal cancer, more specifically, a blood marker that can be practically used for clinical diagnosis of renal cancer. The present invention also provides a blood marker that can be practically used for follow-up after treatment such as surgery and during treatment such as medication for renal cancer. A blood marker for renal cancer selected from the group consisting of Galectin-1, Galectin-3, and α-enolase. Galectin-1 and/or Galectin-3 as a blood marker for renal cancer for use in an examination performed before diagnostic imaging. α-Enolase as a blood marker for renal cancer for use in monitoring during and/or after treatment for renal cancer. | 2015-02-12 |
20150044692 | DEVELOPMENT AND USE OF FLUORESCENT PROBES OF UNBOUND BILIRUBIN - Identification and use of proteins fluorescently labeled and that undergo a change in fluorescence index upon binding bilirubin are described. Probes are disclosed which are labeled at a cysteine or lysine residue and also probes labeled at both cysteine and lysine with two different fluorophores. These probes are useful for determination of unbound bilirubin levels in a fluid sample. | 2015-02-12 |
20150044693 | Levetiracetam Immunoassays - Methods, compositions and kits are disclosed directed at levetiracetam derivatives, immunogens, signal generating moieties, antibodies that bind levetiracetam and immunoassays for detection of levetiracetam. | 2015-02-12 |
20150044694 | ENGINEERED ANTIBODY-NANOPARTICLE CONJUGATES - Conjugates of a C-terminal modified diabody and a nanoparticle are provided in which the C-terminal modification introduces a cysteine residue at a C-terminus of the diabody and the diabody is covalently linked to the nanoparticle via a heterobiofunctional linker attached to the introduced cysteine residue. | 2015-02-12 |
20150044695 | Method and a Kit To Detect Malignant Tumors and Provide a Prognosis - A method and kit is provided to quantifying and qualifying exosomes in human cell derived samples or in body fluid based on expression of TM9-superfamily proteins on the exosomes. Furthermore, a method and a kit to diagnose malignant tumors is provided. The disclosure also provides a method to monitor tumor growth. | 2015-02-12 |
20150044696 | MICROFLUIDIC DEVICE FOR SERIAL FLUIDIC OPERATIONS - An integrated microfluidic device for carrying out a series of fluidic operations includes a housing including a plurality of n microfluidic conduits, wherein n is at least three, and a rotating valve having an internal channel with an entrance port and an exit port that are angularly separated. The rotating valve is positionable in a first position to connect two of the n fluidic conduits via the internal channel, and upon rotating the valve to a second position, two other of the n fluidic conduits are connected by the internal channel. The device further may include one or more fluidic chambers in fluid communication with respective fluidic conduits. Fluid contained in one fluidic chamber is transferrable by application of positive or negative gas pressure through associated fluidic conduits into another fluidic chamber via the internal channel. The device may be utilized to perform a variety of fluidic operations. | 2015-02-12 |
20150044697 | OUTER MEMBRANE PROTEIN 18 AS A DIAGNOSTIC MARKER FOR CAMPYLOBACTER - Accurate and fast detection of the presence of | 2015-02-12 |
20150044698 | OUTER MEMBRANE PROTEIN 18 AS A DIAGNOSTIC MARKER FOR CAMPYLOBACTER - Accurate and fast detection of the presence of Campylobacter disease is important for the proper treatment of patients with Campylobacter infection. Present tests depend upon culture of viable bacteria and identification by microscopy, which requires care, skill, and two or more days for conclusive results. The current invention improves the ease of use and overcomes the limitations of loss of viability and delay inherent in Campylobacter bacterial culture and provides a more rapid alternative for the identification and diagnosis of Campylobacter and campylobacteriosis. The invention provides a new method of detecting Campylobacter by utilizing an outer membrane protein (OMP 18) to develop antibodies for use in immunoassays of bacterial cultures or human fecal samples. | 2015-02-12 |
20150044699 | VIBRIO HARVEYI-SPECIFIC BINDING DOWN SYNDROME CELL ADHESION MOLECULE, METHOD FOR IDENTIFICATION THEREOF AND USE THEREOF - The present invention relates to a | 2015-02-12 |
20150044700 | Anti-Gap43 Antibody - An anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated threonine residue at position 89 (T89) from a phosphorylated threonine residue at position 89 (pT89) of mouse GAP43 set forth in SEQ ID NO: 13, and which is capable of specifically detecting a growth cone; an anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated serine residue at position 96 (S96) from a phosphorylated serine residue at position 96 (pS96) of mouse GAP43, and which is capable of specifically detecting a growth cone; an anti-GAP43 antibody which is capable of distinguishing a non-phosphorylated threonine residue at position 172 (T172) from a phosphorylated threonine residue at position 172 (pT172) of mouse GAP43, and which is capable of specifically detecting a growth cone; and an immunological analysis method using these anti-GAP43 antibodies. | 2015-02-12 |
20150044701 | IgA-BINDING PEPTIDE AND IgA PURIFICATION USING THE SAME - This invention provides a peptide that comprises an amino acid sequence consisting of 14 to 18 amino acid residues represented by Formula (I) and is capable of binding to human IgA: | 2015-02-12 |
20150044702 | MEANS AND METHODS FOR ASSESSING DISORDERS RELATED TO IMPAIRED IRON ADSORPTION OR ENERGY METABOLISM - The present invention pertains to the field of diagnostics for iron adsorption disorder or impaired energy metabolism and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing iron adsorption disorder or impaired energy metabolism. It also relates to a method for determining whether a compound is capable of inducing such iron adsorption disorder or impaired energy metabolism in a subject and to a method of identifying a drug for treating iron adsorption disorder or impaired energy metabolism. Furthermore, the present invention relates to a device and a kit for diagnosing iron adsorption disorder or impaired energy metabolism. | 2015-02-12 |
20150044703 | METHODS AND COMPOSITIONS FOR DETECTING ENDOMETRIAL OR OVARIAN CANCER - Some embodiments of the present invention relate to methods and compositions for detecting the presence of cancer. In particular, methods and compositions for detecting endometrial cancer or ovarian cancer are provided. | 2015-02-12 |
20150044704 | MONOCLONAL ANTIBODIES AND DIAGNOSTIC USES THEREOF - The disclosure relates to antibodies to the preferentially expressed antigen in melanoma (PRAME), and the synovial sarcoma X breakpoint 2 (SSX-2) antigens, methods of use, and diagnostic kits thereof. In exemplary embodiments, the disclosure relates to monoclonal antibodies to specific epitopes of the PRAME and SSX-2 antigens and methods of using such antibodies. | 2015-02-12 |
20150044705 | PROTEIN DETECTION USING MODIFIED CYCLODEXTRINS - A method is provided for detecting a protein using a cyclodextrin covalently linked to at least one label. The cyclodextrin can associate with the protein by sequestering an aromatic amino acid side-chain of the protein in its hydrophobic cavity. After contacting the protein with the cyclodextrin, the label can be detected directly or can undergo a chemical interaction with a reagent to form a detectable product. The label can include an indole moiety, which can react with a halo-substituted organic compound upon exposure to UV light and thereby be rendered fluorescent. Alternatively, the label can include a biotin moiety, which can bind to a binding partner such as avidin, or variants thereof, to form a detectable molecular complex. A labeled cyclodextrin can be used in the present methods to detect a protein of interest in an electrophoresis gel or on a blotting membrane. Aromatic amino acid residues of the protein, in particular tryptophan, remain protected from chemical modification due to sequestration by the cyclodextrin, making these methods compatible with downstream applications that require intact protein. Also provided herein are compositions, kits, and electrophoresis gels for use in detecting proteins. | 2015-02-12 |
20150044706 | METHODS AND COMPOSITIONS FOR DIAGNOSIS AND PROGNOSIS OF RENAL INJURY AND RENAL FAILURE - The present invention relates to methods and compositions for monitoring, diagnosis, prognosis, and determination of treatment regimens in subjects suffering from or suspected of having a renal injury. In particular, the invention relates to using assays that detect Beta-2-glycoprotein 1 as a diagnostic and prognostic biomarker in renal injuries. | 2015-02-12 |
20150044707 | At-Home Blood Pregnancy Test Kit - Disclosed is an at-home blood pregnancy test kit that can identify the presence of hCG in a blood sample to detect pregnancy in female mammals. The present kit includes a housing having a sample strip that is in fluid communication with a sample pad for holding a blood sample, a test pad, and a conjugate pad. The housing further includes a test window and a push button for releasing a reagent that can react with hCG. The reagent and the test pad include antibodies that can bind with hCG antigens to detect presence of the same. The test window is adapted to display a symbol that indicates a negative pregnancy test result in the absence of hCG, or display a symbol that indicates a positive pregnancy test result in the presence of hCG. | 2015-02-12 |
20150044708 | Methods for Detecting Oncofetal Fibronectin - Methods and products for the detection of oncofetal fibronectin indicating molecules in samples are provided. Methods for imaging of oncofetal fibronectin are provided. In some methods provided herein, the sample is treated with a reagent and/or contacted with a non-specific binder. Provided are methods for testing subjects to ascertain health and disease status and to assess the risk of developing a disease or condition. Methods for detecting the presence of oncofetal fibronectin indicating molecules by a variety of methods such as immunoassays and mass spectrometry also are provided. Methods and products for detection of oncofetal fibronectin for selection of concepti are | 2015-02-12 |
20150044709 | MEANS AND METHODS FOR DETERMINING NEUROTOXIN ACTIVITY BASED ON A MODIFIED LUCIFERASE - The present invention is concerned with test systems for determining the activity of neurotoxin polypeptides. Specifically, it relates to a polynucleotide encoding a single chain luciferase fusion polypeptide comprising: (i) a LuxB subunit, (ii) a linker comprising a neurotoxin cleavage site, and (iii) a LuxA subunit and a polypeptide encoded by the polynucleotide. Further provided in accordance with the invention are a vector and a host cell comprising the polynucleotide. Moreover, the present invention relates to a method for determining a proteolytically active neurotoxin polypeptide in a sample and a kit for carrying out the method. | 2015-02-12 |
20150044710 | ENZYMATIC SENSORS AND METHODS FOR THEIR PREPARATION AND USE - Disclosed herein are methods, compositions and devices for detecting oxygen in various samples such as environmental and biological samples. | 2015-02-12 |
20150044711 | Glucose Dehydrogenase - A modified pyrroloquinoline quinone glucose dehydrogenase that exhibits a high selectivity for glucose is provided. A modified pyrroloquinoline quinone glucose dehydrogenase is disclosed in which the amino acid residue G at Position 99 of a pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 1, or the amino acid residue G at Position 100 of the pyrroloquinoline quinone glucose dehydrogenase (PQQGDH) represented by SEQ ID NO: 3, is substituted by the amino acid sequence TGZN (where Z is SX, S, or N and X is any amino acid residue). The modified PQQGDH of the present invention may additionally comprise one or more mutations selected from the group consisting of Q192G, Q192A, or Q192S; L193X; E277X; A318X; Y367A, Y367F, or Y367W; G451C; and N452X (where X is any amino acid residue). | 2015-02-12 |
20150044712 | METHOD AND KIT FOR ANALYZING PROTEIN-PROTEIN INTERACTION USING NANOCLUSTER FORMATION - For efficient analysis of a protein-protein interaction, the present disclosure provides a kit for analyzing a protein-protein interaction, the kit including: a 1 | 2015-02-12 |
20150044713 | METHOD - The present invention provides a method of quantifying the activity of a protein modifying enzyme in a sample, comprising: (i) grouping modified peptides from a first sample and modified peptides from a second sample into a single group according to one of the following parameters: (a) modified peptides having a modification site that is modified by the same protein modifying enzyme; or (b) modified peptides having a modification site that is part of the same modification motif; (ii) calculating enrichment of the modified peptides from the first sample compared to the modified peptides from the second sample in the group; and (iii) calculating the statistical significance of said enrichment; wherein a statistically significant enrichment is indicative of a protein modifying enzyme being activated in the first sample compared to the second sample. In some embodiments, the method further comprises identifying modified peptides in a first sample and a second sample using mass spectrometry (MS) prior to step (i). | 2015-02-12 |
20150044714 | sPLA2 MONITORING STRIP - A device and method for determining the presence or absence, or the level of, sPLA2 activity in a fluid sample. The device includes an absorbent matrix that defines a flow path for a fluid sample, a first region of the absorbent matrix for applying a fluid sample, where one of the components selected from a bioactive sPLA2 substrate and a label is dried onto or within the first region of the absorbent matrix, a second region of the absorbent matrix downstream of, and in fluid communication with, the first region for detecting an aggregated reaction product, where the other component not present in the first region is dried onto or within the second region of the absorbent matrix. | 2015-02-12 |
20150044715 | METHOD FOR ANALYZING FORMYL GLYCINE RESIDUE - Disclosed is a method which enables semiquantitative or quantitative determination of the ratio between cysteine and formylglycine residues in a protein. The method includes (a) a step of labeling the protein (i) with a halogen-substituted carboxylic acid, (ii) with a halogen-substituted carboxylic acid amide, and (iii) with a halogen-substituted carboxylic acid and then with hydrazine, or with a halogen-substituted carboxylic acid and then by oximation, (b) a step of digesting each labeled protein to provide a corresponding mixture of peptide fragments, (c) a step of subjecting each mixture to reverse phase chromatography to separate the peptide fragments from each other to produce a chromatogram, (d) a step of comparing the produced chromatograms with each other to identify the peak corresponding to the peptide fragment that contained a cysteine residue and the peak corresponding to the peptide fragment that contained a formylglycine residue. | 2015-02-12 |
20150044716 | MEANS AND METHODS FOR THE DETERMINATION OF (D)-2-HYDROXYGLUTARATE (D2HG) OR (D)-2-HYDROXYADIPIC ACID - The present invention relates to a method for detecting (D)-2-hydroxyglutarate or (D)-2-hydroxyadipic acid in a sample, the method comprising the steps of: a) contacting a sample with a reagent mixture, wherein said reagent mixture comprises: (i) a solvent, (ii) a dye having an oxidized state and a reduced state, wherein the reduced state can be distinguished from the oxidized state and wherein the dye is initially present in the oxidized state, (iii) an electron transfer agent, (iv) a (D)-2-hydroxyglutarate dehydrogenase enzyme, and (v) a cofactor; and b) detecting (D)-2-hydroxyglutarate or (D)-2-hydroxyadipic acid by measuring the production of the reduced state of the dye. The invention further pertains to a method for diagnosing and/or monitoring a (D)-2-hydroxy-glutarate-associated disease in a subject. Encompassed by the invention is also a method for diagnosing a mutation in an isocitrate dehydrogenase (IDH) gene or in a (D)-2-hydroxyglutarate (D2HG) dehydrogenase enzyme gene in a subject. In addition, the invention provides for a kit comprising (i) a solvent, (ii) a dye having an oxidized state and a reduced state, wherein the reduced state can be distinguished from the oxidized state and wherein the dye is initially present in the oxidized state, (iii) an electron transfer agent, (iv) a (D)-2-hydroxyglutarate dehydrogenase enzyme, and (v) a cofactor. | 2015-02-12 |
20150044717 | DEVICES AND METHODS FOR OBSERVING EUKARYOTIC CELLS WITHOUT CELL WALL - The present invention relates to methods and devices for observing eukaryotic cells devoid of cell wall, in particular for observing the cytokinetic ring, the device comprising a plurality of wells suitable for containing only one single eukaryotic cell and characterized in that the dimensions of the wells constrain the cells into an oblong shape with a long axis parallel to the depth of the wells. | 2015-02-12 |
20150044718 | ON-COLUMN ENZYMATIC CLEAVAGE - Herein is reported a method for obtaining a polypeptide by an immobilized metal ion affinity chromatography from a pro-polypeptide that comprise at its N- or C-terminus an metal ion affinity chromatography tag and a protease cleavage site comprising the step of recovering the polypeptide from the immobilized metal ion affinity chromatography column by incubating the bound pro-polypeptide with a protease, whereby the immobilized metal ion affinity chromatography material has been washed at least once with an urea solution. | 2015-02-12 |
20150044719 | METHOD FOR PRODUCING A RECOMBINANT PROTEIN USING A CELL LINE ADAPTED TO A PROTEIN-FREE AND LIPID-FREE MEDIUM - A method for producing a recombinant protein includes steps of: (a) culturing a transformed cell in a protein-free and lipid-free medium containing no exogenous growth factors, in which the transformed cell is produced by transfecting a cell of a cell line derived from Chinese Hamster Ovary (CHO) cells, the cell line is adapted to a protein-free and lipid-free medium, and the cell is capable of proliferating in a suspended state in a protein-free and lipid-free medium containing no exogenous growth factors, with a vector containing a gene coding for the protein to be produced under the control of a promoter operable in the cell, and (b) recovering the protein produced by the transformed cell. | 2015-02-12 |
20150044720 | SERUM-FREE STABLE TRANSFECTION AND PRODUCTION OF RECOMBINANT HUMAN PROTEINS IN HUMAN CELL LINES - The present invention relates to an improved method for the serum-free production of an immortalized human cell line stably transfected under serum-free conditions with a specific vector carrying the gene coding for the protein of interest. Furthermore the invention relates to a production cell line obtained by said method, a production method for said protein of interest utilizing said production cell line, and the specific vector carrying the gene of interest itself. | 2015-02-12 |
20150044721 | METHOD FOR THE PRODUCTION OF POLYPEPTIDES - New methods for the production of recombinant polypeptides from inclusion bodies are disclosed. Modulation of the cell culture conditions positively affects the yield of the recombinant polypeptide in active form. In one aspect, the methods comprise (a) cultivating a host cell at a first temperature, the host cell comprising a nucleic acid encoding a recombinant polypeptide, (b) lowering the cultivation temperature from the first temperature to a second temperature, and (c) cultivating the host cell at the second temperature. | 2015-02-12 |
20150044722 | SIGLEC-15 ANTIBODIES IN TREATING BONE LOSS-RELATED DISEASE - Novel antibodies and antigen binding fragments that specifically binds to Siglec-15 are described herein In some embodiments, the antibodies or antigen binding fragments may block the biological activity of Siglec-15 and are useful in composition for the treatment of bone loss, more particularly in bone diseases that have increased cell surface expression of Siglec-15, such as conditions where there is an increase in the bone degradative activity of osteoclasts The invention also relates to cells expressing the antibodies or antigen binding fragments such as monoclonal, humanized or chimeric antibodies Additionally, methods of detecting and treating bone loss, bone-related diseases or cancer using the antibodies and fragments are also disclosed. | 2015-02-12 |
20150044723 | COFACTOR REGENERATION SYSTEM - The present invention relates to cofactor regeneration systems, components and uses thereof and methods for generating and regenerating cofactors. The cofactor regeneration system comprises a first electron transfer component selected from a polypeptide comprising a NADH:acceptor oxido-reductase or NADPH:acceptor oxido-reductase, a second electron transfer component selected from a hydrogenase moiety and/or non-biological nanoparticles and an electronically conducting surface. The first and second electron transfer components are immobilised on the electrically conducting surface, and the first and second electron transfer components do not occur together in nature as an enzyme complex. | 2015-02-12 |
20150044724 | PRACTICAL METHOD FOR ENZYMATICALLY SYNTHESIZING CYCLIC DI-GMP - A practical method for enzymatically synthesizing c-di-GMP with excellent productivity is provided. A diguanylate cyclase having physical and chemical characteristics (A) to (F): (A) catalytic action on reaction “2 GTP→c-di-GMP”; (B) a molecular weight of 19800±2000; (C) an optimum pH of 7.3 to 9.4; (D) an optimum temperature of 35 to 60° C.; (E) thermal stability as the remaining activity of 90% or higher after heated for 60 minutes under conditions of 50° C. and pH 7.8; and (F) the presence of GGDEF domain and the lack of amino acid sequence KXXD in the i-site. | 2015-02-12 |
20150044725 | METHOD OF SEPARATING NUCLEIC ACIDS - A method of separating nucleic acids from cells, the method comprising incubating a sample comprising cells with a solid substrate that binds to the cells, whereby the cells adhere to the solid substrate; suspending the solid substrate adhered to the cells in a lysis composition comprising about 100 mM to about 300 mM of alkaline metal salt, and having a pH of about 6 to about 8; lysing the cells in the lysis composition to obtain a lysed solution; and obtaining the nucleic acids from the lysed solution; as well as related compositions and kits. | 2015-02-12 |
20150044726 | DEVICE FOR CONTROLLING THERMAL CONVECTION VELOCITY OF BIOCHEMICAL REACTION AND METHOD FOR THE SAME - The present disclosure is related to a device for controlling thermal convection velocity of a biochemical reaction. The thermal convection velocity controlling device includes a base body for disposing a tube which is movable, wherein the tube is filled with a buffer of the biochemical reaction; a heating source located at a bottom of the tube or at a side of the tube to heat the buffer; and a flow rate adjusting apparatus for controlling a thermal convection flow direction of the buffer in the tube, whereby the flow rate adjusting apparatus changes a flow velocity and a flow time of the buffer. The present disclosure is also related to a method for controlling thermal convection velocity of a biochemical reaction using the device. | 2015-02-12 |
20150044727 | Adaptive Thermal Block Temperature Control Method and System - Aspects of the present teachings describe a method and apparatus for automatically controlling a block temperature to reduce undershooting and overshooting of the temperatures of a sample contained in the block and participating in a polymerase chain reaction (PCR). The adaptive thermal block temperature control begins when a sample temperature enters a sample window region between a preliminary setpoint temperature and a target setpoint temperature for the sample. Based on thermodynamic behavior of the sample and the predetermined phase of PCR, predicting a time period measured subsequent to the preliminary setpoint temperature when the sample will reach the target setpoint suitable for the predetermined phase of PCR. During this time period, varying the block temperature ramp rate with a series of cooling and heating changes to ensure the block temperature reaches the target setpoint temperature at approximately the same time as the sample reaches the same. Synchronizing the block temperature and sample temperature to the target setpoint temperature reduces undershooting and overshooting of the sample temperature and increases the speed and efficiency of the overall PCR process as it relates to the thermal cycling operations. | 2015-02-12 |
20150044728 | SACCHARIFYING ENZYME COMPOSITION AND METHOD FOR PRODUCING SACCHARIFIED SOLUTION USING THE SAME - A saccharifying enzyme composition, by which excellent saccharification performance can be attained even with a low usage thereof, as well as a method for producing a saccharified solution using the same are provided. The saccharifying enzyme composition subjects lignocellulose-based biomass as a substrate to a saccharification treatment. The saccharifying enzyme composition comprises an endoglucanase not containing a cellulose-binding domain, a cellobiohydrolase containing a cellulose-binding domain, and a β-glucosidase containing a cellulose-binding domain. | 2015-02-12 |
20150044729 | BIOMASS PROCESSING SYSTEM, SACCHARIDE SOLUTION PRODUCTION METHOD USING BIOMASS FEEDSTOCK, ALCOHOL PRODUCTION METHOD - A biomass processing system includes: a hydrolysis processing unit that decomposes, under a high-temperature/high-pressure condition, biomass feedstock in a processing tank having a gas-liquid interface, and removes a lignin component and a hemicellulose component; a biomass solid content discharge unit that discharges a biomass solid content | 2015-02-12 |
20150044730 | SUGAR PREPARATION PROCESS BY ENZYMATICALLY HYDROLYZING SWEET POTATO DREG - The present invention relates to the field of biology, in particular to a sugar preparation method by using biomass sweet potato dregs for microbial fermentation as a sugar source. The liquid sugar product prepared with the method of the present invention essentially comprises the ingredient of glucose. The method of the present invention is of simple process, high specificity, good product quality and high yield, and solves the serious environmental pollution problem of the sweet potato dregs, thus having a good industrial application prospect. | 2015-02-12 |
20150044731 | PSYCHROPHILIC ENZYMES COMPOSITIONS AND METHODS FOR MAKING AND USING SAME - Enzyme compositions with enhanced enzyme activity and/or thermophilic and psychrophilic stability are described. Additionally, methods and kits for making and using the enzyme compositions are provided. | 2015-02-12 |
20150044732 | Stabilization of Alpha-Amylases Towards Calcium Depletion and Acidic PH - The present invention relates to variants of a parent alpha-amylase, the variant having improved stability or activity at low calcium conditions or at low pH. | 2015-02-12 |
20150044733 | FLOCCULATION OF LIGNOCELLULOSIC HYDROLYZATES - A method of separating a lignin-rich solid phase from a solution, comprising: pretreating a lignocellulosic biomass with a pretreatment fluid having to remove soluble components, colloidal material and primarily lignin containing particles; separating the pretreated lignocellulosic biomass from the pretreatment fluid with soluble components, colloidal material and primarily lignin containing particles; flocculating the separated pretreatment fluid with soluble components, colloidal material and primarily lignin containing particles using PEO or CPAM as a flocculating agent; and filtering the flocculated separated pretreatment fluid with soluble components, colloidal material and primarily lignin containing particles to remove agglomerates. | 2015-02-12 |
20150044734 | METABOLIC ENGINEERING OF THE SHIKIMATE PATHWAY - The present disclosure relates to engineered microorganisms that produce amino acids and amino acid intermediates. In particular, the disclosure relates to recombinant nucleic acids encoding operons that increase production of aromatic amino acids and the aromatic amino acid intermediate shikimate; microorganisms with increased production of aromatic amino acids and the aromatic amino acid intermediate shikimate; and methods related to the production of aromatic amino acids, the aromatic amino acid intermediate shikimate, and commodity chemicals derived therefrom. | 2015-02-12 |
20150044735 | Biosynthetic Systems Producing Fungal Indole Alkaloids - The biosynthesis of fungal bicyclo[2.2.2]diazaoctane indole alkaloids with a wide spectrum of biological activities have attracted increasing interest. Their intriguing mode of assembly has long been proposed to feature a non-ribosomal peptide synthetase, a presumed intramolecular Diels-Alderase, a variant number of prenyltransferases, and a series of oxidases responsible for the diverse tailoring modifications of their cyclodipeptide-based structural core. Until recently, the details of these biosynthetic pathways have remained largely unknown due to lack of information on the fungal derived biosynthetic gene clusters. Herein, we report a comparative analysis of four natural product metabolic systems of a select group of bicyclo[2.2.2]diazaoctane indole alkaloids including (+)/(−)-notoamide, paraherquamide and malbrancheamide, in which we propose an enzyme for each step in the biosynthetic pathway based on deep annotation and on-going biochemical studies. | 2015-02-12 |
20150044736 | FUNGAL CUTINASE FROM MAGNAPORTHE GRISEA - Described are compositions and methods relating to a fungal cutinase cloned from | 2015-02-12 |
20150044737 | PRODUCTION OF DOCOSAHEXAENOIC ACID AND/OR EICOSAPENTAENOIC ACID AND/OR CAROTENOIDS IN MIXOTROPHIC MODE BY NITZSCHIA - New strains of microalgae belonging to the | 2015-02-12 |
20150044738 | PRODUCTION OF EICOSAPENTAENOIC AND/OR ARACHIDONIC ACID IN MIXOTROPHIC MODE BY EUGLENA - New strains of microalgae belonging to the | 2015-02-12 |
20150044739 | CANDIDA SAKE STRAIN FOR PRODUCING LONG CHAIN DICARBOXYLIC ACIDS - A strain of Candida sake, CAT H | 2015-02-12 |
20150044740 | YEAST CELL WITH ACTIVATED LACTATE DEHYDROGENASE AND METHOD OF PRODUCING LACTATE USING THE YEAST CELL - A yeast cell comprising LDH from a | 2015-02-12 |
20150044741 | CARBOXYLATE ACIDIFICATION - A method for preparing a carboxylic acid by acidification of a liquid feed including a carboxylate salt, which method includes the steps of providing a liquid feed including magnesium carboxylate; providing a gas feed including gaseous hydrogen chloride; and acidifying the carboxylate to carboxylic acid by bringing the liquid feed into contact with the gas feed, thereby forming a liquid effluent including carboxylic acid and magnesium chloride, wherein the gas feed including gaseous hydrogen chloride is derived from a thermal decomposition step wherein an aqueous liquid including magnesium chloride is subjected to a temperature of at least 300° C., thereby decomposing magnesium chloride into magnesium oxide and hydrogen chloride, thus obtaining a solid including magnesium oxide and a gas comprising gaseous hydrogen chloride. | 2015-02-12 |
20150044742 | PROCESSES FOR STARTING UP AND OPERATING DEEP TANK ANAEROBIC FERMENTATION REACTORS FOR MAKING OXYGENATED ORGANIC COMPOUND FROM CARBON MONOXIDE AND HYDROGEN - Processes for starting up of anaerobic, deep tank fermentation systems to anaerobically bioconvert hydrogen and carbon monoxide in a gaseous substrate stream to oxygenated organic compounds and for steady operation of such fermentation systems. In the processes injectors use a motive liquid to introduce gas substrate as a stable gas-in liquid dispersion into the deep tank fermentation reactor where at least one of:
| 2015-02-12 |
20150044743 | LONG CHAIN ORGANIC ACID BIOPRODUCTION - Method of cell culture, comprising adding a redox active compound with a redox potential of between −0.116 to −0.253 to a culture capable of forming hydrogen via a hydrogenase so that the redox potential is diverted from hydrogen to form a longer chain acids, e.g., butryic acid. | 2015-02-12 |
20150044744 | BIOLOGICAL ALKANE OXIDATION - The invention relates to a method for oxidizing an alkane, comprising contacting the alkane with a type alkB oxidoreductase and using a type alkB oxidoreductase to prepare a mixture of oxidation products of an alkane, wherein the ratio of carboxylic acid to alcohol in the oxidation products is preferably greater than 1:1. | 2015-02-12 |
20150044745 | RECOMBINANT CORYNEBACTERIUM AND A METHOD OF PRODUCING C4 DICARBOXYLIC ACID USING THE SAME - A recombinant | 2015-02-12 |
20150044746 | METHOD OF ENHANCED BIOPRODUCTION - Bio-based renewable 3-hydroxypropionic acid (3-HP) may be produced through fermentation processes utilizing genetically modified microorganisms such as, for example, genetically modified | 2015-02-12 |
20150044747 | METHODS FOR INCREASING PRODUCTION OF 3-METHYL-2-BUTENOL USING FUSION PROTEINS - The invention relates, in part, to nucleic acid constructs, genetically modified host cells and methods employing such constructs and host cells to increase the production of 3-methyl-2-butenol from IPP. Thus, in some aspects, the invention provides a genetically modified host cell transformed with a nucleic acid construct encoding a fusion protein comprising a phosphatase capable of catalyzing the dephosphorylation of dimethylallyl diphosphate (DMAPP) linked to an IPP isomerase capable of converting IPP to DMAPP, wherein the nucleic acid construct is operably linked to a promoter. In some embodiments, the genetically modified host cell | 2015-02-12 |
20150044748 | Method for Processing Plant Remains - The invention relates to a method and to a system for processing plant remains, in particular shells of seeds and nuts, even more in particular shells of cocoa beans, shells of grain seeds, and rice remains. The method comprises the following steps: (i) providing plant remains having a shell portion of at least 20 wt %; and (ii) at least partially hydrolyzing constituents of the plant remains, in particular at least partially hydrolyzing and/or fermenting a carbohydrate, a fat, and/or a protein. A liquid phase having dissolved constituents and a solid phase can subsequently be separated. The solid portion can be used as dietary fiber and the liquid phase can be used as feed for a biogas plant. | 2015-02-12 |
20150044749 | METHODS AND COMPOSITIONS FOR PRODUCING HYDROCARBONS - Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels. | 2015-02-12 |
20150044750 | MICROFLUIDIC VORTEX-ASSISTED ELECTROPORATION SYSTEM AND METHOD - A system and method include delivering cells of interest to multiple traps via a channel connecting the traps, maintaining a vortex flow in the traps to trap the cells of interest in the traps, providing first molecules of interest to the traps, and providing an electric field across the traps to perform electroporation of the first molecules of interest into the cells of interest in the traps. | 2015-02-12 |
20150044751 | PHOTOTHERMAL SUBSTRATES FOR SELECTIVE TRANSFECTION OF CELLS - This invention provides novel tools for surgery on single cells and substrates/devices for delivery of reagents to selected cells. In certain embodiments the substrates comprise a surface comprising one or more orifices, where nanoparticles and/or a thin film is deposited on a surface of said orifice or near said orifice, where the nanoparticles and/or a thin film are formed of materials that heat up when contacted with electromagnetic radiation. In certain embodiments the pores are in fluid communication with microchannels containing one or more reagents to be delivered into the cells. | 2015-02-12 |
20150044752 | Method of Treatment of Mesenchymal Stem Cells - Extracted human mesenchymal cells (MSCs) are placed into the interior space of a closed vessel and exposed to an electric AC current and/or electromagnetic field. The MSCs are also stirred within the vessel to further stimulate the cells, enhancing the osteodifferentiation process during human transplantation of the cells. | 2015-02-12 |
20150044753 | Expression Vector Comprising a Polynucleotide Encoding a Modified Glutamine Synthetase and a Method for Preparing a Target Protein Employing the Same - The present invention relates to a vector comprising a polynucleotide encoding a modified glutamine synthetase (GS), and a method for preparing a target protein employing the same. More particularly, the present invention relates to a modified GS having an increased sensitivity to a glutamine synthetase (GS) inhibitor, a polynucleotide encoding the modified GS, a vector comprising the polynucleotide, a transformant comprising the vector, and a method for preparing a target protein using the transformant. | 2015-02-12 |
20150044754 | POLYPEPTIDES HAVING ALPHA-AMYLASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME - Provided are isolated polypeptides having alpha-amylase activity, catalytic domains, carbohydrate binding domains and polynucleotides encoding the polypeptides, catalytic domains or carbohydrate binding domains. Also provided are nucleic acid constructs, vectors and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains or carbohydrate binding domains. | 2015-02-12 |
20150044755 | PRODUCTION OF MUCONIC ACID FROM GENETICALLY ENGINEERED MICROORGANISMS - This present invention is in the field of producing renewable chemical feedstocks using biocatalysts that have been genetically engineered to increase their ability to convert renewable carbon resources into useful compounds. More specifically, the present invention provides a process for producing muconic acid form renewable carbon resources using a genetically modified organism. | 2015-02-12 |
20150044756 | Methods of increasing secretion of polypeptides having biological activity - The present invention relates to methods for producing a secreted polypeptide having biological activity, comprising: (a) transforming a fungal host cell with a fusion protein construct encoding a fusion protein, which comprises: (i) a first polynucleotide encoding a signal peptide; (ii) a second polynucleotide encoding at least a catalytic domain of an endoglucanase or a portion thereof; and (iii) a third polynucleotide encoding at least a catalytic domain of a polypeptide having biological activity; wherein the signal peptide and at least the catalytic domain of the endoglucanase increases secretion of the polypeptide having biological activity compared to the absence of at least the catalytic domain of the endoglucanase; (b) cultivating the transformed fungal host cell under conditions suitable for production of the fusion protein; and (c) recovering the fusion protein, a component thereof, or a combination thereof, having biological activity, from the cultivation medium. | 2015-02-12 |
20150044757 | Methods and Systems for Capturing and Storing Carbon Dioxide - Methods and systems for capturing and storing carbon dioxide are disclosed. In some embodiments, the methods include the following: mixing materials including magnesium or calcium with one or more acids and chelating agents to form a magnesium or calcium-rich solvent; using the organic acids derived from biogenic wastes as acids or chelating agents; generating carbonate ions by reacting a gas including carbon dioxide with a carbonic anhydrase biocatalyst; reacting the solvent with the carbonate ions to form magnesium or calcium carbonates; recycling a solution containing the biocatalyst after forming magnesium or calcium carbonates for re-use in the generating step; using the magnesium and calcium carbonates as carbon neutral filler materials and using the silica product as green filler materials or inexpensive absorbents. | 2015-02-12 |
20150044758 | Apparatus for and Method of Processing Biological Samples - The present invention provides systems, devices, apparatuses and methods for automated bioprocessing. Examples of protocols and bioprocessing procedures suitable for the present invention include but are not limited to immunoprecipitation, chromatin immunoprecipitation, recombinant protein isolation, nucleic acid separation and isolation, protein labeling, separation and isolation, cell separation and isolation, food safety analysis and automatic bead based separation. In some embodiments, the invention provides automated systems, automated devices, automated cartridges and automated methods of western blot processing. Other embodiments include automated systems, automated devices, automated cartridges and automated methods for separation, preparation and purification of nucleic acids, such as DNA or RNA or fragments thereof, including plasmid DNA, genomic DNA, bacterial DNA, viral DNA and any other DNA, and for automated systems, automated devices, automated cartridges and automated methods for processing, separation and purification of proteins, peptides and the like. | 2015-02-12 |
20150044759 | BIOLOGICAL SENSING STRUCTURES - A biological sensing structure includes a mesa integrally connected a portion of a substrate, wherein the mesa has a top surface and a sidewall surface adjacent to the top surface. The biological sensing structure includes a first light reflecting layer over the top surface and the sidewall surface of the mesa. The biological sensing structure includes a filling material surrounding the mesa, wherein the mesa protrudes from the filling material. The biological sensing structure includes a stop layer over the filling material and a portion of the first light reflecting layer. The biological sensing structure includes a second light reflecting layer over a portion of the stop layer and a portion of the top surface of the mesa. The biological sensing structure includes an opening in the second light reflecting layer to partially expose the top surface of the mesa. | 2015-02-12 |
20150044760 | FLUID MIXING AND DELIVERY IN MICROFLUIDIC SYSTEMS - The specification generally discloses systems and methods for mixing and delivering fluids in microfluidic systems. The fluids can contain, in some embodiments reagents that can participate in one or more chemical or biological reactions. Some embodiments relate to systems and methods employing one or more vent valves to controllably flow and/or mix portions of fluid within the microfluidic system. Advantageously, fluid control such as a sequence of fluid flow and/or a change in flow rate, can be achieved by opening and closing one or more vent valves and by applying a single source of fluid flow (e.g., a vacuum) operated at a substantially constant pressure. This can simplify the operation and use of the device by an intended user. | 2015-02-12 |
20150044761 | FOOD SAFETY DETECTION DEVICE AND MANUFACTURING METHOD FOR THE SAME - A food safety detection device includes a xylem fiber substrate, which is configured with a sampling portion and a reaction portion. The reaction portion includes at least one chemical reagent. The sampling portion absorbs a test sample. The test sample moves on the xylem fiber substrate to the reaction portion and reacts with the chemical reagent. A manufacturing method for the food safety detection device is also disclosed. The food safety detection device is advantageous for easy operation, safety and rapid analysis. | 2015-02-12 |
20150044762 | Method and device for determining microbial pollution level in different environments and processes - The present invention provides a device for detecting aerobic bacteria, anaerobic bacteria and fungi in a targeted material which comprises of a plurality sided slide, and a vial, wherein a first side of said plurality sided slide is coated with a first predetermined medium for aerobic bacteria growth and a second side of said plurality sided slide is coated with a second predetermined medium for fungal (mold and yeast) growth, and wherein at the bottom of said vial a third predetermined medium is embedded to determine a level of presence of anaerobic bacteria. | 2015-02-12 |
20150044763 | FRET MEASUREMENT DEVICE AND FRET MEASUREMENT METHOD - FRET measurement uses a FRET probe that includes a probe element X labeled with a donor fluorescent substance and a probe element Y labeled with an acceptor fluorescent substance and enables FRET to occur when the probe element X and the probe element Y approach to each other or bind together. A test sample as a measuring object in FRET measurement contains a test object about which it is unknown whether or not it has an approaching/binding property of allowing the probe element X and the probe element Y to approach to each other or bind together or a separating property of separating from each other the probe element X and the probe element Y that are in a state where they adjoin each other or bind together. A plurality of sets of a fluorescence lifetime τ | 2015-02-12 |
20150044764 | BIOCHEMICAL ASSAY CARTRIDGE - The present invention relates to a biochemical assay cartridge. More particularly, the present invention provides a biochemical assay cartridge including an insertion-type solution cartridge and a reaction cartridge receiving the insertion-type solution cartridge, in which the solution cartridge is inserted into the reaction cartridge to catch a protruding protection film inducement unit by a latch in the reaction cartridge and thus break the protection film inducement unit and a protection film for sealing a reaction solution storage unit attached to the solution cartridge is automatically detached to discharge the reaction solution from the solution cartridge to the reaction cartridge. The biochemical assay cartridge according to the present invention is automatically injected into the reaction cartridge while a sample to be subjected to assay is inserted, through the insertion-type solution cartridge, into the reaction cartridge, and thus user convenience and operability of the assay cartridge are improved. | 2015-02-12 |
20150044765 | CELL CRYOPRESERVATION TOOL - A cell cryopreservation tool has a cell holding member having a body part and a cell holding part and a tubular accommodation member, closed at one end thereof, which is capable of accommodating the cell holding member. The cell holding part of the cell holding member has a long and narrow cell attaching and holding portion. The cell attaching and holding portion has a heat conductor extended in a longitudinal direction thereof. When the cell holding member is accommodated in the tubular accommodation member, the heat conductor is capable of contacting an inner surface of the tubular accommodation member. | 2015-02-12 |
20150044766 | SIMIAN ADENOVIRUS AND HYBRID ADENOVIRAL VECTORS - The present invention provides recombinant adenoviral vectors, immunogenic compositions thereof and their use in medicine, and methods for generating recombinant adenoviral vectors. In particular, the present invention provides an adenovirus vector comprising a capsid derived from chimpanzee adenovirus AdY25, wherein said capsid encapsidates a nucleic acid molecule comprising an exogeneous nucleotide sequence of interest. | 2015-02-12 |
20150044767 | NUCLEATED-CELL CAPTURING FILTER AND NUCLEATED-CELL PREPARATION METHOD USING SAME - The present invention has an object to solve the problem in a method for separating nucleated cells from a cell-containing fluid using a cell separation filter. Specifically, the present invention provides a cell separation filter and a method for preparing cells using a cell separation filter, which are capable of reducing unnecessary cell contamination in a nucleated cell fraction without needs of designing non-woven fabrics to suit cell species to be recovered. The present invention also provides a cell separation filter and a method for preparing cells using the filter capable of improving the recovery yield of nucleated cells. The present invention provides a cell separation filter comprising: a container having an inlet and an outlet, an adsorbent filled in the container, and a partition having an opening, the partition separating the adsorbent. The present invention also provides a method for preparing nucleated cells including the steps of: introducing a cell-containing fluid through the inlet of the cell separation filter to contact the cell-containing fluid with the adsorbent; and recovering a nucleated cell fraction from the filter. | 2015-02-12 |
20150044768 | METHODS AND COMPOSITIONS FOR PRODUCTION OF RECOMBINANT PROTEIN IN HBX-EXPRESSING MAMMALIAN CELLS - The method of the invention provides for producing a heterologous protein in mammalian host cells having nucleic acid encoding Hepatitis B X protein and the heterologous protein, by growing mammalian host cells selected from the group consistng of HKB11, CHO, BHK21, C2C12, and HEK293 cells, by growing mammalian host cells in non-adherent suspension culture, or by growing mammalian host cells which contain nucleic acid providing exogenous X-box Binding Protein, XBP1s. The conditions should be such that HBx, exogenous XBP1s if present, and the heterologous protein are expressed by the mammalian cells. The invention includes compositions for carrying out the method. | 2015-02-12 |
20150044769 | CELL LINE ADAPTED TO A PROTEIN-FREE AND LIPID-FREE MEDIUM, A METHOD FOR PRODUCING THE CELL LINE, AND A MEDIUM FOR THE CELL LINE - A cell of a cell line adapted to a protein-free and lipid-free medium, which is derived from CHO cells, can be stably used for production of recombinant proteins and can proliferate in a suspended state in a protein-free and lipid-free medium containing no exogenous growth factors. A method for adapting CHO cells by using a protein-free and lipid-free medium and a medium used for the method. | 2015-02-12 |
20150044770 | METHOD FOR SELECTIVE CELL ATTACHMENT/DETACHMENT, CELL PATTERNIZATION AND CELL HARVESTING BY MEANS OF NEAR INFRARED RAYS - The present invention relates to a method for selective cell attachment/detachment, cell patternization and cell harvesting by means of near infrared rays. More particularly, conducting polymers or metal oxides having exothermic characteristics upon irradiation of near infrared light is used as a cell culture scaffold, thus selectively attaching/detaching cells without an enzyme treatment. The scaffold has an effect of promoting proliferation or differentiation of stem cells, and therefore, can be used as a stem cell culture scaffold. The scaffold enables cell attachment/detachment without temporal or spatial restrictions, thus enabling cell patternization. | 2015-02-12 |
20150044771 | Novel FRT Recombination Sites and Methods of Use - Methods and compositions using populations of randomized modified FRT recombination sites to identify, isolate and/or characterize modified FRT recombination sites are provided. The recombinogenic modified FRT recombination sites can be employed in a variety of methods for targeted recombination of polynucleotides of interest, including methods to recombine polynucleotides, assess promoter activity, directly select transformed organisms, minimize or eliminate expression resulting from random integration into the genome of an organism, such as a plant, remove polynucleotides of interest, combine multiple transfer cassettes, invert or excise a polynucleotide, and identify and/or characterize transcriptional regulating regions are also provided. | 2015-02-12 |
20150044772 | CRISPR/CAS SYSTEM-BASED NOVEL FUSION PROTEIN AND ITS APPLICATIONS IN GENOME EDITING - An inactive CRISPR/Cas system-based fusion protein and its applications in gene editing are disclosed. More particularly, chimeric fusion proteins including an inCas fused to a DNA modifying enzyme and methods of using the chimeric fusion proteins in gene editing are disclosed. The methods can be used to induce double-strand breaks and single-strand nicks in target DNAs, to generate gene disruptions, deletions, point mutations, gene replacements, insertions, inversions and other modifications of a genomic DNA within cells and organisms. | 2015-02-12 |
20150044773 | ANALYSIS DEVICE, SPECIMEN SAMPLING IMPLEMENT AND ANALYSIS PROCESS - An analysis device | 2015-02-12 |
20150044774 | METHOD FOR QUANTIFYING CHOLESTEROL IN HIGH DENSITY LIPOPROTEIN 3 - Disclosed is the provision of a method for quantifying HDL3 in a test sample without requiring a laborious operation. The method for quantifying cholesterol in high-density lipoprotein 3 comprises allowing a surfactant(s) which specifically react(s) with a high-density lipoprotein 3 to react with a test sample and quantifying cholesterol, and the surfactant(s) is(are) at least one selected from the group consisting of polyoxyethylene polycyclic phenyl ether and polyoxyethylene styrenated phenyl ether. | 2015-02-12 |
20150044775 | Measurement Method and Device, For Determining Degree of Engine Oil Dilution by FAME - For checking engine oil, especially containing rate of FAME (Fatty Acid Methyl Ester), we provide a measuring device including a cylindrical and upright analysis vessel having a reading scale with which a surface boundary is measured. In the vessel, the engine oil is mixed with reagents including alcohol(s) and a demulsifier. And the decreased volume is checked as volume of the FAME contained in the engine oil. | 2015-02-12 |
20150044776 | pH SENSORS - Provided herein are fluorescent sensor agents, and methods of use and manufacture thereof. In particular, sensor agents are provided that exhibit a detectable change in fluorescence (e.g., fluorescence intensity) upon alteration of the pH of the surrounding environment (e.g., upon moving from one pH environment to another). | 2015-02-12 |
20150044777 | VISUAL INDICATION TEST KIT - A visual indication test kit is provided. The visual indication test kit includes a single collector including a plurality of plugs, wherein the plurality of plugs are configured to serve as extraction points for analytes, and mix a plurality of reagents with a plurality of analytes extracted onto the plurality of plugs, wherein the mixing of the plurality of reagents and the plurality of analytes facilitates performing a plurality of visual indications. | 2015-02-12 |
20150044778 | EXTERNAL FIELD -FREE MAGNETIC BIOSENSOR - A biosensor includes a magnetic structure having grooved surface to biologically bond magnetic labels to a biological substance within the grooves. The grooves are positioned within the magnetic structure so that stray magnetic fields from the magnetic structure magnetize magnetic labels within the groove. The magnetic labels may be magnetic nanoparticles or magnetic microbeads. The techniques may reduce or eliminate the usage of any external magnetic field generator, e.g., electromagnets or current lines. | 2015-02-12 |
20150044779 | STABILIZED LOW AFFINITY CONFORMATION OF INTEGRINS FOR DRUG DISCOVERY - The methods and compositions described herein are based, in part, on the discovery that the introduction of a disulfide bond into an integrin polypeptide by the substitution of at least one cysteine residue in the polypeptide permits stabilization of the integrin in a “closed/inactive” state. This stabilizing disulfide bond permits integrins to be screened for a candidate molecule that can bind to the closed state. In particular, this approach can be used to screen for agents that bind to the closed state of an integrin polypeptide, and are useful as therapeutic treatments to prevent integrin activation. | 2015-02-12 |
20150044780 | MULTI-APPLICATION APPROACH FOR PHOTOMETRIC DETERMINATION OF AN ANALYTE IN A FLUID SAMPLE ON AN AUTOMATED ANALYZER - A method for determining the amount of specific analyte of a sample which may show interferences by photometric assays, wherein the analyte is quantified from the change in the optical signal of the reaction mixture after the interaction of the analyte with analyte specific reagents. Multiple calibration curves are generated for multiple wavelengths for the specific analyte. An interference test is performed simultaneously to the determination of the specific analyte, for quantifying the amount of interfering substances present in the sample. The amount of each interfering substances is compared to predetermined cut-off values. The optical signal for the specific analyte is measured in the reaction mixture at multiple wavelengths over the complete reaction time, and a calibration curve is selected depending on the interfering substances. The amount of specific analyte is quantified by comparison with the selected calibration curve for the chosen wavelengths. | 2015-02-12 |
20150044781 | METHOD OF FORMING MAGNETIC MEMORY DEVICES - Provided is a method of forming a magnetic memory device. A first magnetic layer, a tunnel barrier, and a second magnetic layer are deposited on a substrate. The second magnetic layer, the tunnel barrier, and the first magnetic layer are etched to form magnetic tunnel junction structures. An ion beam etching process is performed using an oxygen-containing source gas to remove etching by-products on sidewalls of the magnetic tunnel junction structure and to oxidize the sidewalls of the magnetic tunnel junction structures. | 2015-02-12 |
20150044782 | FABRICATION PROCESS AND LAYOUT FOR MAGNETIC SENSOR ARRAYS - A magnetic sensor includes a plurality of groups, each group comprising a plurality of magnetic tunnel junction (MTJ) devices having a plurality of conductors configured to couple the MTJ devices within one group in parallel and the groups in series enabling independent optimization of the material resistance area (RA) of the MTJ and setting total device resistance so that the total bridge resistance is not so high that Johnson noise becomes a signal limiting concern, and yet not so low that CMOS elements may diminish the read signal. Alternatively, the magnetic tunnel junction devices within each of at least two groups in series and the at least two groups in parallel resulting in the individual configuration of the electrical connection path and the magnetic reference direction of the reference layer, leading to independent optimization of both functions, and more freedom in device design and layout. The X and Y pitch of the sense elements are arranged such that the line segment that stabilizes, for example, the right side of one sense element; also stabilizes the left side of the adjacent sense element. | 2015-02-12 |
20150044783 | METHODS OF ALLEVIATING ADVERSE STRESS EFFECTS ON A WAFER, AND METHODS OF FORMING A SEMICONDUCTOR DEVICE - A method of forming a forming a semiconductor device comprises forming at least one semiconductor device structure over a surface of a wafer. An opposing surface of the wafer is subjected to at least one chemical-mechanical polishing process to form a modified opposing surface of the wafer comprising at least one recessed region and at least one elevated region. Additional methods of forming a semiconductor device, and methods of reducing stress on a wafer are also described. | 2015-02-12 |