06th week of 2022 patent applcation highlights part 28 |
Patent application number | Title | Published |
20220042016 | Identification of a JAK2 Mutation in Polycythemia Vera - The present invention concerns the V617F variant of the protein-tyrosine kinase JAK2, said variant being responsible for Vaquez Polyglobulia. The invention also relates to a first intention diagnostic method for erythrocytosis and thrombocytosis allowing their association with myeloproliferative disorders, or to the detection of the JAK2 V617F variant in myeloproliferative disorders allowing their reclassification in a new nosological group. | 2022-02-10 |
20220042017 | METHODS FOR IMPROVING THE HEALTH OF PORCINE SPECIES BY TARGETED INACTIVATION OF CD163 - The present disclosure relates methods and compositions useful for prevention of porcine reproductive and respiratory syndrome virus (PRRSv) in animals, including animals of the species | 2022-02-10 |
20220042018 | APTAMERS AND THE USE THEREOF IN THE TREATMENT OF CANCER - The present invention is based on the identification, by the inventors, of a series of new aptamers containing a minimum common motif and binding specifically, at the intracellular level, to protein kinases MNK1, more specifically to MNK1a and MNK1b, respectively, particularly with a higher affinity for MNK1b than for MNK1a. Said binding between the aptamer and the protein MNK1, as illustrated in the examples of the present invention, provides an effective therapy in the treatment of cancer, more specifically in the treatment of breast cancer, even more specifically in the treatment of triple-negative breast cancer. Therefore, these molecules are useful for the production of new pharmaceutical compositions for oncological treatment. | 2022-02-10 |
20220042019 | COMPOSITIONS AND METHODS OF USE FOR SMALL-MOLECULE REGULATION OF CRISPR-CAS9 ACTIVITY USING RNA APTAMERS - Provided herein are single guide RNAs (sgRNAs) that comprise aptamer sequences and related compositions and methods. Also provided herein are methods of selecting inducible sgRNAs that comprise aptamer sequences. | 2022-02-10 |
20220042020 | PHARMACEUTICAL COMPOSITION THAT INHIBITS PRODUCTION OF HEPATITIS B VIRUS PROTEIN AND SCREENING METHOD - An object of the present invention is to provide a pharmaceutical composition useful as a novel anti-hepatitis B virus agent and a screening method. According to the present invention, there is provided a pharmaceutical composition that inhibits a production of a hepatitis B virus protein, in which the pharmaceutical composition inhibits an expression or a function of an RNA-binding protein that binds to at least one sequence selected from a hepatitis B virus RNA sequence corresponding to an enhancer I region sequence on a hepatitis B virus genome DNA or a hepatitis B virus RNA sequence corresponding to an enhancer II region sequence on the hepatitis B virus genome DNA. | 2022-02-10 |
20220042021 | RNAi-MEDIATED INHIBITION OF HIF1A FOR TREATMENT OF OCULAR ANGIOGENESIS - RNA interference is provided for inhibition of HIF1A mRNA expression for treating patients with ocular angiogenesis, particularly for treating retinal edema, diabetic retinopathy, sequela associated with retinal ischemia, posterior segment neovascularization (PSNV), and neovascular glaucoma, and for treating patients at risk of developing such conditions. | 2022-02-10 |
20220042022 | ANTISENSE OLIGONUCLEOTIDES FOR MODULATING HTRA1 EXPRESSION - The present invention relates to antisense oligonucleotides (oligomers) that are complementary to HTRA1, leading to modulation of the expression of HTRA1. Modulation of HTRA1 expression is beneficial for a range of medical disorders, such as macular degeneration, e.g. age-related macular degeneration. | 2022-02-10 |
20220042023 | Genetic and Pharmacological Transcriptional Upregulation of the Repressed FXN Gene as a Therapeutic Strategy for Friedreich Ataxia - Methods of treating subjects having disorders associated with mutations in the FXN gene and/or having reduced expression of frataxin protein, e.g., Friedreich ataxia (FA). Generally, the methods include administering a therapeutically effective amount of an agent that increases expression of frataxin protein as described herein, e.g., an inhibitor of a FXN Repressing Factor (FXN-RF). | 2022-02-10 |
20220042024 | GENETICALLY MODIFIED BACTERIA PRODUCING THREE DNA REPAIR ENZYMES AND METHOD FOR THE EVALUATION OF DNA REPAIR ACTIVITY - Three recombinant | 2022-02-10 |
20220042025 | COMPOSITIONS AND METHODS FOR CONVERTING STYRENE TO BIODEGRADABLE ALTERNATIVES - Provided are nucleic acids and vectors that collectively encode various gene products related to converting styrene to polyhydroxybutyrate (PHB). In some embodiments, the nucleic acids and vectors collectively encode a styrene monooxygenase polypeptide, a flavin reductase polypeptide, a styrene-oxide isomerase polypeptide, and a phenylacetaldehyde dehydrogenase polypeptide, an acetyl-CoA C-acetyltransferase polypeptide, a 3-ketoacyl-ACP reductase polypeptide, a class I poly(R)-hydroxyalkanoic acid synthase polypeptide, and optionally an influx porin polypeptide. Also provided are systems and methods for producing PHB from styrene, methods and systems for remediating polystyrene waste. In some embodiments, the systems are in vivo systems. | 2022-02-10 |
20220042026 | METHODS FOR OPTOGENETIC MANIPULATION OF STOMATAL FUNCTION - The invention relates to methods of increasing stomatal function in plants, which as a result leads to an increase in carbon assimilation and/or water use efficiency and ultimately an increase in yield. In particular the methods of the invention relate to the expression of light-gated potassium channels in the stomatal complex. Also described are genetically altered plants characterised by the above phenotype as well as methods of producing such plants. | 2022-02-10 |
20220042027 | FRUIT-SPECIFIC PROMOTERS - The present disclosure provides genetic constructs containing a promotor that is useful in driving fruit-specific expression in plants. Further provided are expression vectors, transgenic plants, and plant parts containing such genetic constructs, as well as uses thereof. | 2022-02-10 |
20220042028 | PRODUCTION OF DHA AND OTHER LC PUFAS IN PLANTS - The invention provides recombinant host organisms genetically modified with a polyunsaturated fatty acid (PUFA) synthase system and one or more accessory proteins that allow for and/or improve the production of PUFAs in the host organism. The present invention also relates to methods of making and using such organisms as well as products obtained from such organisms. | 2022-02-10 |
20220042029 | MANIPULATING PLANT SENSITIVITY TO LIGHT - The present disclosure identifies new genes which have the potential to increase broad acre yield in crops. This disclosure is based upon our fundamental knowledge of light signal transduction and our understanding of tile roles these genes play in regulating plant growth and development in response to light. Transgenic plants with gain- or loss-of-function of one of these genes, or in combination, are expected to show significant improvements in broad acre yield and stress tolerance. | 2022-02-10 |
20220042030 | A METHOD TO IMPROVE THE AGRONOMIC CHARACTERISTICS OF PLANTS - A method to improve the agronomic characteristics of plants by genetically transforming the plant with a nucleic acid sequence encoding RAMOSA1 transcription factor, where the plant is selected from the group consisting of gramineous monocotyledons of the BOP clade, monocotyledons-non-grasses and dicotyledons. In addition, sequences of DNA, cDNA, protein, cells, uses, genetic transformation method of plants and transformed plants are described. | 2022-02-10 |
20220042031 | Carbon Fixation Systems in Plants and Algae - Provided are heterologous nucleic acid constructs, vectors and methods for elevating cyclic electron transfer activity, improving carbon concentration, and enhancing carbon fixation in C3 and C4 plants, and algae, and producing biomass or other products from C3 or C4 plants, and algae, selected from among, for example, starches, oils, fatty acids, lipids, cellulose or other carbohydrates, alcohols, sugars, nutraceuticals, pharmaceuticals, fragrance and flavoring compounds, and organic acids, as well as transgenic plants produced thereby. These methods and transgenic plants and algae encompass the expression, or overexpression, of various combinations of genes that improve carbon concentrating systems in plants and algae, such as bicarbonate transport proteins, carbonic anhydrase, light driven proton pump, cyclic electron flow regulators, etc. | 2022-02-10 |
20220042032 | NUCLEOTIDE SEQUENCES AND POLYPEPTIDES ENCODED THEREBY USEFUL FOR MODIFYING PLANT CHARACTERISTICS - Isolated polynucleotides and polypeptides encoded thereby are described, together with the use of those products for making transgenic plants with increased tolerance to abiotic stress (e.g., high or low temperature, drought, flood). | 2022-02-10 |
20220042033 | COMPOSITION AND METHOD FOR CONFERRING AND/OR ENHANCING TOLERANCE AGAINST HERBICIDES BY USING VARIANTS OF PPO - Provided is a technology for conferring more enhanced tolerance of plants and/or algae against herbicides and/or more greatly enhancing tolerance by using amino acid variants of protoporphyrinogen IX oxidases derived from microorganisms. | 2022-02-10 |
20220042034 | Rust Resistance Gene - The present invention relates to a plant which has integrated into its genome an exogenous polynucleotide encoding a polypeptide which confers resistance to at least one strain of | 2022-02-10 |
20220042035 | NON-VIRAL DNA VECTORS AND USES THEREOF FOR ANTIBODY AND FUSION PROTEIN PRODUCTION - The application describes ceDNA vectors having linear and continuous structure for delivery and expression of a transgene. ceDNA vectors comprise an expression cassette flanked by two ITR sequences, where the expression cassette encodes a transgene. Some ceDNA vectors further comprise cis-regulatory elements, including regulatory switches. Further provided herein are methods and cell lines for reliable gene expression in vitro, ex vivo and in vivo using the ceDNA vectors. Provided herein are method and compositions comprising ceDNA vectors useful for the expression of an antibody or fusion protein in a cell, tissue or subject. Such antibodies or fusion proteins can be expressed for treating disease or alternatively, for the production of antibodies or fusion proteins in a commercial setting. | 2022-02-10 |
20220042036 | METHODS AND COMPOSITIONS FOR PREPARING SURFACTANT PROTEIN D (SP-D) - Some embodiments of the methods and compositions provided herein relate to the preparation surfactant protein-D (SP-D). Some embodiments include the expression of human SP-D in certain cell lines, and the purification of human SP-D from such cell lines. Some embodiments include the preparation of certain oligomeric forms of human SP-D. | 2022-02-10 |
20220042037 | Adult Stem Cell Line Introduced with Hepatocyte Growth Factor Gene and Neurogenic Transcription Factor Gene with Basic Helix-Loop-Helix Motif and Uses Thereof - The present invention relates to an adult stem cell line introduced with an HGF gene and a neurogenic transcription factor gene of a bHLH family, a preparation method of the adult stem cell line, and a method for treating neurological diseases comprising the step of transplanting the adult stem cell line to a subject having neurological diseases. The adult stem cells according to the present invention, which are introduced with an HGF gene and a neurogenic transcription factor gene of a bHLH family, can be used to treat chronic impairment caused by cell death following stroke. Thus, the adult stem cells can be developed as a novel therapeutic agent or widely used in clinical trial and research for cell replacement therapy and gene therapy that are applicable to neurological diseases including Parkinson's disease, Alzheimer disease, and spinal cord injury as well as stroke. | 2022-02-10 |
20220042038 | NANOTRANSPOSON COMPOSITIONS AND METHODS OF USE - Disclosed are compositions comprising a first nucleic acid sequence comprising: (a) a first inverted terminal repeat (ITR), (b) a second ITR and (c) an intra-ITR sequence, wherein the intra-ITR sequence comprises a transposon sequence, and a second nucleic acid sequence comprising an inter-ITR sequence, wherein the length of the inter-1TR sequence is between 1 and 600 nucleotides, inclusive of the endpoints. Preferably, the compositions are nanotransposons. | 2022-02-10 |
20220042039 | IMPROVED LENTIVIRAL VECTOR - The invention belongs to the field of biomedicine. Specifically, the present invention relates to improved t lentiviral vector, and preparation method and uses thereof. Specifically, the present invention relates to a lentiviral vector especially suitable for preparing a therapeutic T cell. | 2022-02-10 |
20220042040 | DUAL VECTOR SYSTEM FOR IMPROVED PRODUCTION OF PROTEINS IN ANIMAL CELLS - The present invention refers to a dual vector system for production of one or more recombinant proteins in cells of insect origin comprising a first viral vector comprising a T7 promoter operably linked to a targeting sequence comprising a non-coding sequence and which expression can suppress one or more proteins essential for virus production, followed by a T7 termination sequence, and a second viral vector comprising a promoter operably linked to a T7 RNA polymerase encoding sequence, and at least one gene sequence located on the first and/or second vector encoding one or more recombinant proteins of interest. The invention further refers to recombinant insect cells comprising the dual vector system and methods for producing recombinant proteins using said dual vector CA system. | 2022-02-10 |
20220042041 | CELL SPECIFIC TRANSCRIPTIONAL REGULATORY SEQUENCES AND USES THEREOF - New synthetic expression cassettes comprising a minimal promoter and a cell-specific enhancer for expression of a nucleic acid of interest in one or more specific cell subtypes are disclosed. Vectors and host cells comprising such synthetic expression cassettes are also disclosed. The application also discloses methods for expressing a nucleic acid of interest, such as a nucleic acid encoding a chimeric antigen receptor (CAR), in a cell and for treating diseases or conditions such as cancers and genetic diseases using the synthetic expression cassettes, vectors and cells. | 2022-02-10 |
20220042042 | ANELLOSOMES AND METHODS OF USE - This invention relates generally to anellosomes and compositions and uses thereof. | 2022-02-10 |
20220042043 | RECOMBINANT VECTORS FOR THE LONG TERM TREATMENT OF MUCCHOPOLYSACHARIDOSIS - The present invention provides new pharmaceutical co positions for the tong term treatment of diseases and specially, for use as tong term treatment of mucopolysaccharidoses type IIIA. | 2022-02-10 |
20220042044 | REDIRECTION OF TROPISM OF AAV CAPSIDS - The disclosure relates to compositions, methods, and processes for the preparation, use, and/or formulation of adeno-associated virus capsid proteins, wherein the capsid proteins comprise targeting peptide inserts for enhanced tropism to a target tissue. | 2022-02-10 |
20220042045 | EXPRESSION CASSETTES FOR GENE THERAPY VECTORS - The present invention relates to a recombinant expression cassette comprising a polynucleotide encoding a SMN protein. This cassette can be included in a gene therapy vector and used in a method for the treatment of spinal muscular atrophy (SMA). | 2022-02-10 |
20220042046 | TUMOR-NAVIGATING, SELF-ERADICATING, TRAIL-ARMED SALMONELLA FOR PRECISION CANCER THERAPY - The present disclosure relates to genetically modified strains of | 2022-02-10 |
20220042047 | COMPOSITIONS AND METHODS FOR MODIFYING A TARGET NUCLEIC ACID - The present disclosure provides a system for editing genomic DNA, the system comprising an asymmetric donor DNA template; and methods of editing genomic DNA involving use of an asymmetric donor DNA template. The present disclosure provides a system for editing genomic DNA, the system comprising a Cas9 polypeptide with reduced enzymatic activity; and methods of editing genomic DNA involving use of a Cas9 polypeptide with reduced enzymatic activity. | 2022-02-10 |
20220042048 | NONVIRAL GENERATION OF GENOME EDITED CHIMERIC ANTIGEN RECEPTOR T CELLS - Described herein are non-viral, ex vivo methods of site-specifically inserting a transgene containing a chimeric antigen receptor (CAR) gene into a T cell genome by introducing into a population of unmodified T cells a Cas9 ribonucleoprotein (RNP) and a non-viral double-stranded homology-directed repair (HDR) template, to provide genome-edited T cells. The Cas9 ribonucleoprotein includes a Cas9 protein and a guide RNA that directs double stranded DNA cleavage of a cleavage site in a T cell expressed gene. The non-viral double-stranded HDR template comprises the synthetic DNA sequence flanked by homology arms that are complementary to sequences on both sides of the cleavage site in the T cell expressed gene. The transgene is specifically integrated into the cleavage site of the T cell expressed gene created by the Cas9 RNP in the genome-edited T cells, and the cells are then cultured. | 2022-02-10 |
20220042049 | ENDOGENOUS LIPASE FOR METAL REDUCTION IN DISTILLERS CORN OIL - Provided herein are compositions, methods, systems associated with propagation and fermentation, and co-products of biochemical production processes, for example, a DCO co-product resulting from converting oil containing grains into bio chemicals via fermentation in the presence of endogenous esterase. The DCO resulting from the processes exhibits lower metal ion content relative to a DCO obtained in the absence of endogenous fermentation with an esterase such as a lipase. The DCO is useful as a feedstock for the production of renewable diesel. | 2022-02-10 |
20220042050 | Process For Xylitol Production From Xylose - The present invention discloses a process of xylitol production from pure xylose, xylose containing liquid extract from the acid pretreated biomass (LEPB) and co-production of ethanol and xylitol from dilute acid pretreated lignocellulosic biomass by natural | 2022-02-10 |
20220042051 | LIPOXYGENASE-CATALYZED PRODUCTION OF UNSATURATED C10-ALDEHYDES FROM POLYUNSATRURATED FATTY ACIDS - Described herein are methods for the lipoxygenase (LOX)-catalyzed production of aliphatic unsaturated C | 2022-02-10 |
20220042052 | LABYRINTHULID MICROORGANISM CAPABLE OF PRODUCING MICROBIAL OIL, MICROBIAL OIL, METHODS FOR PRODUCING SAID MICROORGANISM AND FOR PRODUCING SAID MICROBIAL OIL, AND USES OF SAID MICROORGANISM AND SAID MICROBIAL OIL - A method for producing a microbial oil includes steps of: genetically modifying a labyrinthulid by disrupting and/or silencing a gene, or by transforming another gene in addition to the disruption and/or gene silencing of the gene, and culturing the labyrinthulid, such that a fatty acid composition accumulated in the labyrinthulid comprises an increased EPA content; and collecting the microbial oil having the increased EPA content from the labyrinthulid. The labyrinthulid before the modification is selected from (A) a labyrinthulid belonging to the genus | 2022-02-10 |
20220042053 | PRODUCTION METHOD FOR RARE FATTY ACID USING NOVEL ENZYME, AND NOVEL RARE FATTY ACID - A method for producing a hydroxylated fatty acid by a hydration reaction using a novel enzyme derived from the genus | 2022-02-10 |
20220042054 | OIL OF MICROORGANISMS RICH IN DOCOSAHEXAENOIC ACID - The present invention relates to an oil of microorganisms rich in docosahexaenoic acid (DHA, C22:6n3), comprising more than 60% of DHA relative to the total mass of fat and to the use thereof for human or animal feed, in particular for feeding infants, children, or pregnant or lactating women. | 2022-02-10 |
20220042055 | METHOD OF REDUCING AROMATIC NITRO COMPOUNDS - A method for reducing a substrate selected from 2-methyl-5-nitropyridine and methyl 4-(2-fluoro-3-nitrobenzyl)piperazine-1-carboxylate is provided catalysed by a nitroreductase and a disproportionation agent. | 2022-02-10 |
20220042056 | ISOLATION OF FUNGAL MELANIN AND USES IN EXTERNAL RADIATION SHIELDING AND HEAT CAPTURE - Described are methods including cell wall-associated melanin extraction and extracting melanin from microbes producing extracellular vesicles comprising melanin. Further described are composition comprising melanin, melanin coated articles and methods of coating an article. Uses of melanin in methods of heat generation and microwave radiation protection are also described. | 2022-02-10 |
20220042057 | PRODUCTION OF BIOACTIVE OLIGOSACCHARIDES - Methods of generating oligosaccharides are provided. | 2022-02-10 |
20220042058 | Exopolysaccharide from Rhodopseudomonas palustris and Method for Preparing and Use Thereof - The invention discloses an exopolysaccharide from | 2022-02-10 |
20220042059 | METHOD FOR SYNTHESIZING SINGLE-STRANDED DNA - A method for synthesizing single-stranded DNA, specifically a process for producing single-stranded DNA without base mutations, is provided, by which single-stranded DNA is produced by uracil-specific excision reagent (USER)-mediated self-looping of double-stranded DNA combined with rolling circle replication. | 2022-02-10 |
20220042060 | BIOSYNTHESIS PRODUCTION OF STEVIOL GLYCOSIDES AND PROCESSES THEREFORE - The present invention relates to the production of steviol glycoside rebaudiosides D4, WB1 and WB2 and the production of rebaudioside M from Reb D4. | 2022-02-10 |
20220042061 | METHOD FOR THE BIOSYNTHESIS OF DIOSMIN AND/OR HESPERIDIN IN A MICROORGANISM - The present invention relates to a recombinant microorganism which is modified to be capable of producing diosmin and hesperidin and to the use thereof for producing diosmin and/or hesperidin. | 2022-02-10 |
20220042062 | ENGINEERED ARYL SULFATE-DEPENDENT ENZYMES - The present invention provides several non-naturally occurring sulfotransferase enzymes that have been engineered to react with aryl sulfate compounds as sulfo group donors, instead of the natural substrate 3′-phosphoadenosine 5′-phosphosulfate (PAPS), and with heparosan-based polysaccharides, particularly heparan sulfate, as sulfo group acceptors. Each of the engineered sulfotransferase enzymes have a biological activity characterized by the position within the heparosan-based polysaccharide that receives the sulfo group, including glucosaminyl N-sulfotransferase activity, hexuronyl 2-O sulfotransferase activity, glucosaminyl 6-O sulfotransferase activity, or glucosaminyl 3-O sulfotransferase activity. Methods of using the engineered sulfotransferases to produce sulfated heparosan-based polysaccharides, including polysaccharides having anticoagulant activity, are also provided. | 2022-02-10 |
20220042063 | NOVEL PEPTIDE FOR ENHANCING EXPRESSION EFFICIENCY OF TARGET PROTEIN, AND FUSION PROTEIN COMPRISING SAME - The present invention relates to a novel peptide or a partial sequence thereof for enhancing expression efficiency of a target protein, and a fusion protein comprising the same. The novel peptide according to the present invention can enhance expression efficiency of a target protein, and furthermore, the peptide can also be applied to a solubility-enhancing fusion protein in order to enhance solubility of the target protein, so that solubility as well as expression efficiency of the target protein is enhanced, which allows such a peptide to be usefully used for production of a recombinant target protein. | 2022-02-10 |
20220042064 | CARBON-SOURCE REGULATED PROTEIN PRODUCTION IN A RECOMBINANT HOST CELL - A recombinant host cell comprising an endogenous gene encoding a FLO8 protein comprising the amino acid sequence identified as SEQ ID NO:1 or a homologue thereof, which host cell is engineered by one or more genetic modifications to reduce expression of said gene compared to the host cell prior to said one or more genetic modifications, and which host cell comprises a heterologous expression cassette comprising a gene of interest (GO!) under the control of an expression cassette promoter (ECP) which ECP is repressible by a non-methanol carbon source, and a method of producing a protein of interest using said recombinant host cell. | 2022-02-10 |
20220042065 | TISSUE TESTER - A tissue tester for providing an indication of the presence of disease including a first paper tissue sheet; and a filter patch treated with a color changing indicator affixed to the first paper tissue sheet. The tester may also consist of a single sheet of filter paper treated with a solution of turmeric and alcohol. The color indicator changes from yellow to brown when disease is indicated in respiratory secretions. | 2022-02-10 |
20220042066 | SYSTEMS AND METHODS FOR MICROCOLONY GROWTH AND MICROBIAL CELL CHARACTERIZATION - An integrated fluidic device is employed to perform microbial cell separation, in situ microcolony growth, and optional identification and antimicrobial susceptibility testing. While the integrated fluidic device is maintained in a closed state, microbial cell separation is performed to provide a microbial cell suspension that is contacted with a solid phase growth medium. A liquid component of the suspension is removed, thereby retaining microbial cells on the growth medium for incubation, growth, and subsequent harvesting and characterization. In some embodiments, antimicrobial susceptibility testing is performed by contacting growth media with a solid support having an antimicrobial agent provided thereon, such that the antimicrobial agent diffuses into a subregion of the growth medium that is accessible through an aperture surrounded, at least in part, by the solid support. Microbial cells retained on the surface of the subregion may be assessed for growth or inhibition in the presence of the antimicrobial agent. | 2022-02-10 |
20220042067 | METHOD FOR ASSISTING PREDICTION OF SHUNT TROUBLE AND KIT THEREFOR - The present invention provides a method for assisting a prediction of how likely a shunt trouble occurs, comprising a step of determining that the shunt trouble likely occurs, when a mevalonic acid concentration in a sample derived from a hemodialyzed patient is equal to or more than a preset cutoff value or increases with time. | 2022-02-10 |
20220042068 | Methods of Administering 3,4-Diaminopyridine - Provided herein are methods of determining NAT acetylation status of a subject with a 3,4-DAP-sensitive disease, methods of selecting a dose of 3,4-DAP or a pharmaceutically acceptable salt thereof adjusted to a subject's acetylation status, methods of administering 3,4-diaminopyridine or a pharmaceutically acceptable salt thereof to a patient in need thereof, and methods of treating 3,4-DAP sensitive diseases. | 2022-02-10 |
20220042069 | METHOD FOR DETERMINING LEVELS OF INTERACTIONS BETWEEN BIOMOLECULES - The invention relates to a method for determining levels of interactions between biomolecules, such as proteins, in a sample, comprising providing a first and a second information carrying (IC) oligonucleotide, wherein the first and second IC oligonucleotide are attached, covalently or non-covalently, to a first and a second affinity reagent, such as antibodies, that have the capacity to bind to a first and a second biomolecule, wherein the first and second IC oligonucleotide each comprises at least one single-stranded stretch that is complementary to a part of another oligonucleotide, thereby, upon hybridisation of the at least one single-stranded stretch in at least one of the first and second IC oligonucleotides to its complementary part of another oligonucleotide, enabling measurement of the relative proportion of interacting and non-interacting first and second biomolecules in the sample at a single cell or single molecular level. Further, the invention relates to kits including necessary oligonucleotides and reagents to carry out the method of the invention. | 2022-02-10 |
20220042070 | METHODS AND SYSTEMS FOR READING DNA STORAGE GENES - Methods and systems for use in reading DNA storage genes generally include removing one or more linking symbols from a first strand of a DNA storage gene, introducing a test symbol pool to the DNA storage gene, and replacing a data symbol in the first strand of the DNA storage gene with a single stranded test symbol from the test symbol pool when the linking symbol and data symbol of the single stranded test symbol are complimentary to an adjacent linking symbol and data symbol in the second strand of the DNA storage gene. The DNA storage gene is then scanned to identify locations on the DNA storage gene where the linking symbol is double stranded or single stranded. The locations where double stranded linking symbols are detected are then used, along with the composition of the test symbol pool, to read the DNA storage gene. | 2022-02-10 |
20220042071 | DETECTION METHOD USING KIT FOR DETECTING PLURALITY OF TARGET NUCLEIC ACIDS - The present invention provides a method for detecting multiple target nucleic acids, the method making it possible to amplify a plurality of genes using one reaction vessel containing therein one type of reaction solution and further using a single label. Computation is performed in accordance with the following formulas for every specific cycle and/or every cycle during amplification reaction using a kit for detecting the multiple target nucleic acids. (Formula 1): f1[n]=fhyb.1[n]/fden.1[n], (Formula 1′): f2[n]=fhyb.2[n]/fden.2[n], (Formula 2): Fr[n]=(a−f2 [n])/(a−f1 [n]).fhyb.1 [n]: Fluorescence intensity value in elongation step of first target nucleic acid detecting step. The same hereinafter. | 2022-02-10 |
20220042072 | METHODS FOR NUCLEIC ACID ANALYSIS - The present disclosure provides methods and processes for increasing the efficiency and accuracy of nucleic acid sequencing using techniques such as polymerase chain reaction (PCR). The methods described herein can be used to achieve clonal amplification even with a greater than Poisson distribution of beads and/or nucleic acid templates into an emulsion. A PCR method may comprise generating a partition (e.g., a droplet) comprising at least two beads and/or at least two nucleic acid molecules and generating clonal amplification products corresponding to the nucleic acid molecule, at least a subset of which may be attached to a bead. | 2022-02-10 |
20220042073 | LED DRIVEN PLASMONIC HEATING APPARATUS FOR NUCLEIC ACIDS AMPLIFICATION - Systems and methods for plasmonic heating by combined use of thin plasmonic film-based 2D and 3D structures and a light-emitting diode (LED) for nucleic acids amplification through fast thermal cycling of polymerase chain reaction (PCR) are described. | 2022-02-10 |
20220042074 | DNA-BARCODED NUCLEOSOMES FOR CHROMATIN MAPPING ASSAYS - The present invention relates to DNA-barcoded recombinant nucleosomes and polynucleosomes that have been engineered for use as spike-in controls for chromatin accessibility assays, chromatin mapping assays, e.g., using tethered enzymes, as well as other chromatin assays. The invention further relates to methods of using the engineered DNA-barcoded recombinant nucleosomes in chromatin accessibility assays, chromatin mapping assays, as well as other chromatin assays. | 2022-02-10 |
20220042075 | METHODS, COMPOSITIONS, AND DEVICES FOR SOLID-STATE SYNTEHSIS OF EXPANDABLE POLYMERS FO RUSE IN SINGLE MOLECULE SEQUENCINGS - Methods, compositions and devices for single molecule sequencing are provided, particularly for solid-state synthesis and processing of expandable polymers (e.g., Xpandomers), as well as methods and compositions for producing new expandable polymer constructs that provide more accurate sequence information when passed through a nanopore sensor. | 2022-02-10 |
20220042076 | PORTABLE MOLECULAR DIAGNOSTIC DEVICE AND METHODS FOR THE DETECTION OF TARGET VIRUSES - A method includes coupling a molecular diagnostic test device to a power source. A biological sample is conveyed into a sample preparation module. The device is then actuated by only a single action to cause the device to perform the following functions without further user action. First, the device heats the sample via a heater of the sample preparation module to lyse a portion of the sample. Second, the device conveys the lysed sample to an amplification module and heats the sample within a reaction volume of the amplification module to amplify a nucleic acid thereby producing an output solution containing a target amplicon. The device then reacts, within a detection module, each of (i) the output solution and (ii) a reagent formulated to produce a signal that indicates a presence of the target amplicon within the output solution. A result associated with the signal is then read. | 2022-02-10 |
20220042077 | MDA USING BEAD OLIGONUCLEOTIDE - Improved multiple displacement amplification (MDA) reagents and methods are provided. | 2022-02-10 |
20220042078 | IDENTIFICATION AND ANALYSIS OF MICROBIAL SAMPLES BY RAPID INCUBATION AND NUCLEIC ACID ENRICHMENT - The disclosure relates to methods, compositions, and kits for the identification and analysis of microorganisms in a sample using nucleoside or nucleotide analogs. | 2022-02-10 |
20220042079 | Binder Selection Using Capollary Electrophoresis - A method of capillary electrophoresis is provided for binder selection. In an embodiment, a capillary electrophoresis method comprises selecting an electroosmotic flow (EOF) in a capillary such that at least one target-binder (TB) complex has a target-binder velocity vector (vTB) co-directed with an electric field vector (E) and at least one non-binder (N) has a non-binder velocity vector (vN) in the opposite direction to the electric field vector (E); introducing a sample comprising the at least one target-binder (TB) complex, the at least one non-binder (N), and at least one running buffer into a capillary inlet of the capillary; applying an electric field directed from the capillary inlet to a capillary outlet of the capillary to separate the at least one target-binder (TB) complex from the at least one non-binder (N); and detecting the at least one target-binder complex. | 2022-02-10 |
20220042080 | NUCLEIC ACID SEQUENCE MEASUREMENT DEVICE - In a case where the target ( | 2022-02-10 |
20220042081 | DETECTION OF ANALYTES BY ENZYME-MEDIATED STRAND DISPLACEMENT REACTIONS - The subject invention pertains to composition and methods of using said composition as an in vitro biosensor of small molecules in biological and/or environmental samples using enzyme-assisted nucleic acid reactions. The methods and compositions can be used to sense and/or transduce the signal of a sensing event mediated by allosteric proteins, endonucleases and nucleic acid reactions. This invention allows the rapid development and setup of one-pot assays to provide results in minutes. The methods and compositions may be used to generate an electrochemical, fluorescent, colorimetric, and/or luminescent output and the methods can be performed in different modalities, including a solution-based or paper-based assay. | 2022-02-10 |
20220042082 | AMPLIFICATION AND ANALYSIS OF SELECTED TARGETS ON SOLID SUPPORTS - Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. Targets are circularized by hybridization to probes followed by ligation of the ends of the target to form a closed circle. The targets are then used as template for extension of an array bound probe resulting in extended probes having multiple copies of the target. The extended probes can then be analyzed. The methods may be used for genotyping, sequencing and analysis of copy number. | 2022-02-10 |
20220042083 | METHODS FOR IN SITU TRANSCRIPTOMICS AND PROTEOMICS - Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a cell. | 2022-02-10 |
20220042084 | SPATIAL SEQUENCING - Disclosed herein, inter alia, are compositions and methods of use thereof for interrogating a sample comprising a cell. | 2022-02-10 |
20220042085 | System and method of nucleic acid amplification for point of collection - A system for nucleic acid amplification is to synthesize amplified target nucleic acids or determine the presence of target nucleic acid. The mobile device of the system may be implemented with software for analyzing the reaction or optionally delivering the information of a sample to a cloud. Therefore, the system can provide corresponding genetic information of organism, cancer cells or viruses of interest. The information may include gene expression levels of interest, DNA identity of samples as well as treatment suggestion and professional lists for consulting. The system could also optionally be used with a mobile device to amplify the target nucleic acid for the downstream sequencing or measurement. | 2022-02-10 |
20220042086 | Methods and Devices for Performing Real Time Digital PCR - Disclosed are devices that can perform multiple independent digital PCRs with real-time monitoring capability. The device comprises multiple PCR mini-reactors thermally coupled with its own temperature control element, a detection unit, and a motor for moving the PCR mini-reactors or the detection unit. The real-time digital PCR device can simultaneously perform multiple digital PCRs, generate amplification curves of thousands and millions of individual PCR processes, evaluate binary readouts based on the kinetic properties of individual amplification curves, and identify different target sequences based on the amplification curves. Methods of using the real-time digital PCR device to detect target nucleic acids and count circulating tumor cells are also disclosed. | 2022-02-10 |
20220042087 | POLYNUCLEOTIDE BARCODES FOR LONG READ SEQUENCING - Provided herein are methods of making, amplifying, and sequencing tagged nucleic acid complements, compositions including interposing oligonucleotide barcodes, and kits useful in obtaining long-range sequence data. | 2022-02-10 |
20220042088 | METHODS AND COMPOSITIONS FOR PATHOGEN DETECTION IN PLANTS - The technology relates in part to methods and compositions for detecting one or more pathogens in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in plants. In some aspects, the technology relates to methods and compositions for detecting hops latent viroid in | 2022-02-10 |
20220042089 | NUCLEIC ACID AMPLIFICATION AND IDENTIFICATION METHOD - A method for generating labelled amplification fragments of a nucleic acid template including the steps of providing a template nucleic acid, annealing at least one oligonucleotide primer to the template nucleic acid, elongating the at least one oligonucleotide primer in a template specific manner thereby creating an elongation product, wherein said elongating reaction stops when the elongation product reaches the 5′ end of the template nucleic acid or a nucleic acid elongation stopper that is annealed to the template nucleic acid downstream of the elongation product, providing an adaptor nucleic acid that has an identification sequence on its 5′ end, wherein the identification sequence does not hybridize to the elongation stopper when in contact thereto, and ligating the adaptor nucleic acid at its 5′ end to the 3′ end of the elongation product, thereby generating a labelled amplification fragment. | 2022-02-10 |
20220042090 | PROGRAMMABLE RNA-TEMPLATED SEQUENCING BY LIGATION (rSBL) - A method for determining the presence or absence of variant ribonucleic acid molecules in a population of ribonucleic acid molecules, wherein the reference sequence of the variant ribonucleic acid molecules is known, the method comprising: (a) interrogating the population of ribonucleic acid molecules with a plurality of primer molecules and a plurality of probes so as to saturate the population of ribonucleic acid molecules with the probes and primer molecules such that the probes and primer molecules are adjacent to one another when hybridized to their respective complimentary sequences on the ribonucleic acid molecules, (b) ligating the probes to their respective adjacent primer molecules so as to form ligated nucleic acid molecules, and (c) detecting the presence of ligated nucleic acid molecules that are complementary to a sequence that differs from the reference sequence, thereby determining the presence or absence of one or more variant ribonucleic acid molecules in the population of ribonucleic acid molecules. | 2022-02-10 |
20220042091 | Mitochondrial DNA Quality Control - The present disclosure provides methods of identifying unreliable biological samples that may be mislabeled or contaminated, by determining the heteroplasmy and homoplasmy of mitochondrial DNA present in the biological samples. | 2022-02-10 |
20220042092 | METHOD FOR SEQUENCING A DIRECT REPEAT - Described herein is a method of sequencing a template that comprises a direct repeat, comprising: (a) in the same reaction, hybridizing a primer to a first site that is upstream of the first repeat sequence and hybridizing a primer to a second site that is upstream of the second repeat sequence, wherein the first and second sites are: (i) upstream of the first and second repeat sequences, respectively, and (ii) equidistant from the first and second repeat sequences; and (b) subjecting the hybridization product of (a) to a sequencing-by-synthesis sequencing reaction to produce a sequence read that comprises a combination of the first and second repeat sequences. | 2022-02-10 |
20220042093 | Preserving Genomic Connectivity Information in Fragmented Genomic DNA Samples - A method of sequencing a target nucleic acid polymer by (a) modifying a target nucleic acid polymer to produce a modified nucleic acid polymer; (b) producing fragments of the modified nucleic acid polymer, wherein the fragments are attached to locations on a solid support surface (c) determining nucleotide sequences from the fragments at the locations; and (d) producing a representation of the nucleotide sequence for the target nucleic acid polymer based on the nucleotide sequences from the fragments and the relative distances between the locations on the solid support surface. | 2022-02-10 |
20220042094 | GENOME FRACTIONING - Disclosed herein are compositions and methods related to the elimination of molecules of a selected sequence from a nucleic acid sample or from an sequence dataset resulting from the sequencing of a sample, for example to exclude such molecules from downstream analysis or sequencing, or to exclude such sequences from a downstream data set. | 2022-02-10 |
20220042095 | METHODS AND COMPOSITIONS FOR MONITORING AND ENHANCING EARLY EMBRYO DEVELOPMENT - Method for determining developmental fate of early embryos comprises measuring the expression level of a gene selected from the group consisting of CDKIV1C, IGF2R, MAGEL2, MKRN3, NAP1L5, NDN, PEG3, PHLDA2, TSSC4, and UBE3A genes. Also disclosed is a method for improving pregnancy rate, wherein early embryos whose expression level of the MKRN3, NDN, PEG3, PHLDA2, TSSC4, or UBE3A gene is not increased, or the expression level of the CDKIV1C, IGF2R, MAGEL2, or NAP1L5 gene is not decreased, are selected for planting into a suitable uterus for further development. Also disclosed are methods for increasing the likelihood of an early embryo to develop successfully into full-term pregnancy, wherein a suitable amount of siRNA corresponding to the PHLDA2 gene is injected into a fertilized egg which is in turn cultured further and planted into a suitable uterus. | 2022-02-10 |
20220042096 | FLEXIBLE AND HIGH-THROUGHPUT SEQUENCING OF TARGETED GENOMIC REGIONS - The disclosure pertains to materials and methods for capturing a target genomic region, comprising hybridizing an extension probe and a ligation probe to target sequences that flank the target genomic region; elongating the 3′ end of the extension probe until the 3′ end of the elongated extension probe is adjacent to the 5′ end of the ligation probe; and ligating the 3′ end of the elongated extension probe with the 5′ end of the ligation probe to produce a ligated probe. The ligated probe can be PCR amplified to produce copies of the target genomic region that can be detected or sequenced. Certain embodiments of the invention also provide methods of producing double stranded probes suitable for capturing and analyzing both strands of a target genomic region in a double stranded genomic DNA. The invention also provides kits for performing the methods disclosed herein. | 2022-02-10 |
20220042097 | IN-SITU SPATIAL TRANSCRIPTOMICS AND PROTEOMICS - The present disclosure relates to systems and method of in-situ tissue profiling. Methods for spatiotemporal processing of a sample, capturing molecules of interest, and correlating cells in the sample to the capture molecules are provided. | 2022-02-10 |
20220042098 | METHODS FOR PROFILING AND QUANTITATING CELL-FREE RNA - The invention generally relates to methods for assessing the health of a tissue by characterizing circulating nucleic acids in a biological sample. According to certain embodiments, methods for assessing the health of a tissue include the steps of detecting a sample level of RNA in a biological sample, comparing the sample level of RNA to a reference level of RNA specific to the tissue, determining whether a difference exists between the sample level and the reference level, and characterizing the tissue as abnormal if a difference is detected. | 2022-02-10 |
20220042099 | MOLECULAR AND FUNCTIONAL CHARACTERIZATION OF EARLY-STAGE PARKINSON'S DISEASE AND TREATMENTS THEREIN - A method and composition for detecting, diagnosing, prognosing or monitoring a subject suspected of having or having Parkinson's disease by detecting miRNAs and microbial RNAs in saliva. | 2022-02-10 |
20220042100 | QUANTIFYING FOREIGN DNA IN LOW-VOLUME BLOOD SAMPLES USING SNP PROFILING - Provided herein are methods for quantifying foreign cell-free DNA (cfDNA) via SNP profiling of low-volume blood sample. The methods allow for monitoring the status of organ transplant rejection through analysis of small volumes of patient capillary blood samples collected non-invasively with fingersticks or other devices. The methods also allow for guiding the dosage of immunosuppressant and for preparing for a new organ transplant in case of imminent organ failure. | 2022-02-10 |
20220042101 | Treatment Of Obesity With G-Protein Coupled Receptor 75 (GPR75) Inhibitors - The present disclosure provides methods of treating subjects having obesity, methods of identifying subjects having an increased risk of developing obesity, methods of detecting human G-protein coupled receptor 75 variant nucleic acid molecules and variant polypeptides, and GPR75 variant nucleic acid molecules and variant polypeptides. | 2022-02-10 |
20220042102 | MIRNA FINGERPRINT IN THE DIAGNOSIS OF LUNG CANCER - The present invention provides novel methods for diagnosing diseases based on the determination of specific miRNAs that have altered expression levels in disease states compared to healthy controls. | 2022-02-10 |
20220042103 | METHODS FOR NON-INVASIVE PRENATAL PLOIDY CALLING - The present disclosure provides methods for determining the ploidy status of a chromosome in a gestating fetus from genotypic data measured from a sample of DNA from the mother of the fetus and from the fetus, and from genotypic data from the mother and optionally also from the father. The ploidy state is determined by using a joint distribution model to create a set of expected allele distributions for different possible fetal ploidy states given the parental genotypic data, and comparing the expected allelic distributions to the pattern of measured allelic distributions measured in the mixed sample, and choosing the ploidy state whose expected allelic distribution pattern most closely matches the observed allelic distribution pattern. In an embodiment, the mixed sample of DNA may be preferentially enriched at a plurality of polymorphic loci in a way that minimizes the allelic bias. | 2022-02-10 |
20220042104 | SYSTEMS AND METHODS TO DETECT RARE MUTATIONS AND COPY NUMBER VARIATION - The present disclosure provides a system and method for the detection of rare mutations and copy number variations in cell free polynucleotides. Generally, the systems and methods comprise sample preparation, or the extraction and isolation of cell free polynucleotide sequences from a bodily fluid; subsequent sequencing of cell free polynucleotides by techniques known in the art; and application of bioinformatics tools to detect rare mutations and copy number variations as compared to a reference. The systems and methods also may contain a database or collection of different rare mutations or copy number variation profiles of different diseases, to be used as additional references in aiding detection of rare mutations, copy number variation profiling or general genetic profiling of a disease. | 2022-02-10 |
20220042105 | COMPOSITIONS AND METHODS FOR SCREENING AND DIAGNOSIS OF PROSTATE CANCER - The present invention provides methods of screening for and diagnosing prostate cancer and methods of choosing a therapeutic for prostate cancer based on using KDM5D expression level to identify which patients with hormone sensitive prostate cancer benefit from primary castration and taxane and who with castration resistant prostate cancer would benefit from docetaxel plus an androgen receptor antagonists added to the ongoing castration. The disclosure also provides methods of screening for and diagnosing prostate cancer and methods of choosing a therapeutic for prostate cancer based on a lower KDM5D expression having a more aggressive clinical course of prostate cancer in human patients. | 2022-02-10 |
20220042106 | SYSTEMS AND METHODS OF USING CELL-FREE NUCLEIC ACIDS TO TAILOR CANCER TREATMENT - This disclosure relates to systems and methods for assessing disease from cell-free nucleic acids to tailor a treatment. In particular, the systems and methods described herein identify cell-free nucleic acids from a body fluid sample and use the identified cell-free nucleic acids to produce expression signatures that are indicative of disease severity. The expression signatures are correlated with known outcomes to provide prognostic information for the patient, thereby allowing a clinician to tailor a treatment to a predicted disease severity. | 2022-02-10 |
20220042107 | SYSTEMS AND METHODS OF SCORING RISK AND RESIDUAL DISEASE FROM PASSENGER MUTATIONS - In particular, systems and methods of the invention measure amounts of passenger mutations, and optionally driver mutations, to predict risk of cancer-related pathogenicity. Preferably, the mutations are measured from sequences reads. Sequence reads of patient-derived nucleic acids may be compared with one or more references to identify and measure the amount of passenger mutations and optionally driver mutations. The amount of passenger and driver mutations may be correlated to known associations with recurrent and residual disease to predict a risk of cancer recurrence. | 2022-02-10 |
20220042108 | SYSTEMS AND METHODS OF ASSESSING BREAST CANCER - This disclosure relates to systems and methods for providing individualized assessments of breast cancer. The assessments are provided in the format of index scores that are prognostic to disease severity and progression. Methods involve measuring gene expression from a patient sample to create a gene expression signature. The gene expression signature is compared with expression signatures from tumors that are associated with known outcomes. The comparison is used to generate an index score that assigns a risk status to the patient. The index score may indicate, for example, risk of distant metastasis or benefit of chemotherapy. | 2022-02-10 |
20220042109 | METHODS OF ASSESSING BREAST CANCER USING CIRCULATING HORMONE RECEPTOR TRANSCRIPTS - The present disclosure provides systems and methods for analyzing circulating hormone receptor transcripts to provide diagnoses, prognoses, and treatment suggestions for patients afflicted with breast cancer. Circulating transcripts can be obtained from patient samples, including blood samples, without the need for invasive tissue biopsies. This may include expression transcripts obtained from extracellular vesicles. Analysis of hormone receptor expression transcripts may include comparison with expression transcripts of patients with known clinical outcomes. | 2022-02-10 |
20220042110 | DETECTION OF GENE FUSIONS BY INTRAGENIC DIFFERENTIAL EXPRESSION (IDE) USING AVERAGE CYCLE THRESHOLDS - Described herein are methods and kits for detecting the presence or absence of gene dysregulations such as those arising from gene fusions and/or chromosomal abnormalities, e.g. translocations, insertions, inversions and deletions. The methods, compositions and kits are useful for detecting mutations that cause the differential expression of a 5′ portion of a target gene relative to the 3′ region of the target gene. The average expression of the 5′ portion of the target gene is compared with the average expression of the 3′ portion of the target gene to determine an intragenic differential expression (IDE). The IDE can then be used to determine if a dysregulation or a particular disease (or susceptibility to a disease) is present or absent in a subject or sample. | 2022-02-10 |
20220042111 | COMPOSITIONS AND METHODS FOR PERFORMING METHYLATION DETECTION ASSAYS - Provided herein is technology relating to performing methylation assays. In particular, the technology relates to internal controls for methylation assays. | 2022-02-10 |
20220042112 | TARGETED THERAPIES FOR CANCER - Various embodiments provide compositions and methods for detecting cancers containing an NRG1 fusion event and treating a patient with a therapeutic agent that is targeted to the NRG1 fusion. Exemplary compositions for treating cancers containing the NRG1 fusion may comprise therapeutic agents inhibiting Epidermal Growth Factor Receptor and/or ERBB2 such as cetuximab, panitumumab, Sym004, MM-151, mAb 806, mAb 528, MEHD794A, gefitinib, erlotinib, lapatinib, afatinib, PD153035, AG1478, trastuzumab, and pertuzumab. In some embodiments, the therapeutic agent may be a combination of trastuzumab, and pertuzumab. | 2022-02-10 |
20220042113 | METHODS OF MEASURING C19MC miRNA IN A POST-NATAL TISSUE AND USES THEREOF - Provided herein are methods of measuring C19MC miRNA amount and/or expression in a post-natal cell and/or tissue. Provided herein are methods of measuring CpG methylation of the upstream C19MC miRNA promoter region in a post-natal cell and/or tissue. Also provided herein are methods of treating a cell, population thereof, and/or a subject in need thereof by administering a C19MC miRNA inhibitor or CRISPR to suppress the expression of specific miRNA within the C19MC or the entire C19MC cluster, population thereof, and/or the subject in need thereof. | 2022-02-10 |
20220042114 | DISCOVERY OF BIOLOGICAL SIGNATURES OF OPTIMIZED SENSITIVITY AND SPECIFICITY - A bioinformatics method is provided for identifying candidate biological sequences, such as DNA, RNA, and proteins, with high sensitivity and specificity for application in procedures such as PCR and gene and protein sequencing. The method involves categorizing a collection of biological sequences within an out-group and an in-group, identifying the intersection between the in-group and the out-group, the union of the out-group, and a relative complement of sequences that are members of the in-group, but not the out-group. A biological signature for a species of interest with high sensitivity and specificity will be a member of the relative complement that has an out-group frequency of zero. | 2022-02-10 |
20220042115 | ROTAVIRUS GENOTYPE DETECTION METHOD, AND GENE AMPLIFICATION PRIMER SET USED IN SAME - The present invention addresses the problem of finding a new gene detection primer capable of more accurately detecting genotypes of rotavirus A and a detection means using the gene detection primer, and provides a rotavirus genotype detection method, a gene amplification primer set used in this method, and a genotype detection kit. In the rotavirus genotype detection method, (A) using a base sequence at positions from 174 to 834 of a VP7 gene segment of a G1 type strain of the rotavirus A as a base sequence counting reference, sense primers each aligned to one to four kinds of base sequence regions selected from (1) the positions from 802 to 834 (corresponding to G3 type), (2) the positions from 747 to 789 (corresponding to equine-like G3 type), (3) the positions from 603 to 636 (corresponding to G9 type), and (4) the positions from 666 to 711 (corresponding to G12 type) are used as basic gene amplification sense primers, and (B) using the above positions from 883 to 1062 as the counting reference, antisense primers each aligned to a region of the base sequence of the counting reference are used as gene amplification common antisense primers. | 2022-02-10 |