05th week of 2016 patent applcation highlights part 28 |
Patent application number | Title | Published |
20160032253 | ATTENUATION OF INFECTIOUS BRONCHITIS VIRUS VARIANT GA-13 - The present invention provides the isolation and characterization of a new infectious bronchitis virus (IBV) variant, the IBV GA-13 variant, and the production of attenuated isolates thereof, including, but not limited to, the attenuated IBV GA13 isolate 103505 Kd E86, and the use of such IBV isolates in materials and methods for combating infectious bronchitis virus in poultry and reducing the economic impact that infectious bronchitis disease has on poultry production. | 2016-02-04 |
20160032254 | AAV VECTORS PRODUCED BY INSECT CELLS COMPRISING REP52 AND REP78 CODING SEQUENCES WITH DIFFERENTIAL CODON BIASES - The present invention relates to production of proteins in insect cells whereby repeated coding sequences are used in baculoviral vectors. In particular the invention relates to the production of parvoviral vectors that may be used in gene therapy and to improvements in expression of the viral rep proteins that increase the productivity of parvoviral vectors. | 2016-02-04 |
20160032255 | Ketol-Acid Reductoisomerase Enzymes and Methods of Use - Provided herein are polypeptides having ketol-acid reductoisomerase activity as well as microbial host cells comprising such polypeptides. Polypeptides provided herein may be used in biosynthetic pathways, including, but not limited to, isobutanol biosynthetic pathways. | 2016-02-04 |
20160032256 | Cytochrome P450S and Uses Thereof - The invention features isolated cytochrome P450 polypeptides and nucleic acid molecules, as well as expression vectors and transgenic plants containing these molecules. In addition, the invention features uses of such molecules in methods of increasing the level of resistance against a disease caused by a plant pathogen in a transgenic plant, in methods for producing altered compounds, for example, hydroxylated compounds, and in methods of producing isoprenoid compounds. | 2016-02-04 |
20160032257 | AGENT AND METHOD FOR MODIFYING THE 5' CAP OF RNA - An agent and method for modifying the 5′ cap of RNA, for example for the purposes of isolation and analysis. According to one aspect the invention provides modified enzymes, namely modified trimethylguanosine synthases 2 from | 2016-02-04 |
20160032258 | Methods for Purifying Pertussis Toxin and Peptides Useful Therefor - The present invention relates to reagents and methods for purifying pertussis toxin (PT). | 2016-02-04 |
20160032259 | GLYCOSYLATED VEGF DECOY RECEPTOR FUSION PROTEIN - The present application describes an isolated nucleic acid molecule encoding a polypeptide capable of synchronously binding VEGF polypeptide and placenta growth factor (PIGF) polypeptide comprising a nucleotide sequence encoding a VEGFR1 component. | 2016-02-04 |
20160032260 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 2016-02-04 |
20160032261 | T7 RNA POLYMERASE VARIANTS WITH ENHANCED THERMOSTABILITY - The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous. | 2016-02-04 |
20160032262 | Methods and Compositions for PCR - A modified thermostable Pol B DNA polymerase, produced by a reaction, under essentially aqueous conditions, of a thermostable Pol B DNA polymerase and a modifier reagent of Formula I wherein the reaction results in a thermally reversible inactivation of the thermostable Pol B DNA polymerase activity and the 3′-5′ exonuclease activity, which polymerase is suitable for hot-start PCR. Also disclosed are the method for the modification, a polynucleic acid amplification method and PCR reaction mixture and kit comprising the modified thermostable Pol B DNA polymerase. | 2016-02-04 |
20160032263 | HIV-1 INTEGRASE DERIVED PEPTIDES AND COMPOSITIONS - Isolated peptides comprising sequences derived from the protein integrase of HIV-1, as well as their analogs, mixtures, conjugates with permeability enhancing moieties, and pharmaceutical compositions are disclosed. The peptides and compositions are capable of selectively killing HIV-1 infected cells and are used in treatment of HIV infection and AIDS. | 2016-02-04 |
20160032264 | PREPARATIONS AND METHODS FOR TREATING MALIGNANCIES - Disclosed are therapeutic formulations comprising antibodies against the PEKRAEKIWK (SEQ ID NO:1) epitope of the monomeric isoform of A-protein and a physiologically acceptable carrier. Methods for the treatment of subjects using these therapeutic formulations are also disclosed. | 2016-02-04 |
20160032265 | THERMOSTABLE BETA-XYLOSIDASE BELONGING TO GH FAMILY 3 - A thermostable β-xylosidase including a β-xylosidase catalytic domain, the β-xylosidase catalytic domain including:
| 2016-02-04 |
20160032266 | PRODUCTION OF THERMOLYSIN AND VARIANTS THEREOF AND USE IN LIQUID DETERGENTS - The present invention provides methods and compositions comprising at least one thermolysin-like neutral protease enzyme with improved storage stability and/or catalytic activity. In some embodiments, the thermolysin finds use in cleaning and other applications comprising detergent. In some particularly preferred embodiments, the present invention provides methods and compositions comprising thermolysin formulated and/or engineered to resist detergent-induced inactivation. | 2016-02-04 |
20160032267 | METHODS FOR IMPROVING MULTIPLE PROTEIN PROPERTIES - The present invention provides efficient methods for obtaining a protein with one or more beneficial attributes in industrial, consumer or pharmaceutical applications. In some preferred embodiments, the present invention provides methods for producing superior enzymes for a given application through screening an abbreviated set of candidate enzymes. | 2016-02-04 |
20160032268 | UREASE PURIFICATION FROM JACK BEANS OR OTHER ORGANISMS - A method for the extraction and purification of urease from jack beans or other natural sources of urease. The method provides an efficient way to obtain purified urease from natural sources. The method can include defatting the natural sources of urease, extracting the urease from impurities, and further purification of the extracted urease. | 2016-02-04 |
20160032269 | BUFFERS FOR USE WITH POLYMERASES - Amplification reaction mixtures comprising a cationic surfactant and an anionic surfactant are provided. | 2016-02-04 |
20160032270 | APPARATUS AND PROCESS FOR PRODUCTION OF AN ENCAPSULATED CELL PRODUCT - A process for production of an encapsulated cell product, the process comprises the steps of concentrating cells from a propagation medium using a tangential flow filtration system. Mixing the concentrated cells with an encapsulation medium to form a cell encapsulation mixture. Polymerizing, gelling, or cross-linking the cell encapsulation mixture to form an encapsulated cell product. | 2016-02-04 |
20160032271 | ENCAPSULATION OF BACTERIA AND VIRUSES IN ELECTROSPUN FIBERS - A method of preserving organisms in viable form, the method comprising: suspending organisms in a solution of electrospinnable polymer; drawing droplets of said solution through a spinneret; applying an electrostatic field to said droplets under electrospinning conditions; so as to form fibers having a diameter no greater than about 5 μm within which distinct organisms are encapsulated in viable form. | 2016-02-04 |
20160032272 | PREPARATION METHOD OF YEAST CELL IMMOBILIZATION MEDIUM AND APPLICATION THEREOF - Provided is a preparation method of a yeast cell immobilization medium, which comprises the following steps: (1) boiling a fiber material in boiling water and drying the fiber material; (2) soaking the fiber material in a surface modified aqueous solution with a concentration of 1-100 g/L, using hydrochloric acid to adjust a PH of the solution to 7.0, fully rinsing the fiber material in deionized water and drying the fiber material; (3) soaking the fiber material in a cross-linking agent aqueous solution with a concentration of 1-100 g/L, fully rinsing the fiber material in deionized water and drying the fiber material; and (4) attaching the fiber material to supporting framework. Also provided is the yeast cell immobilization medium prepared using the preparation method and a method for producing ethanol using the yeast cell immobilization medium. | 2016-02-04 |
20160032273 | CHARACTERIZATION OF MRNA MOLECULES - The present invention describes methods for the characterization of mRNA molecules during mRNA production. Characterizing mRNA includes processes such as oligonucleotide mapping, reverse transcriptase sequencing, charge distribution analysis, and detection of RNA impurities. Oligonucleotide mapping includes using an RNase to digest antisense duplexes from an RNA transcript, and then subjecting the digested RNA to reverse phase HPLC, anion exchange HPLC, and/or mass spectrometry analysis. Reverse transcriptase sequencing involves reverse transcription of an RNA transcript followed by DNA sequencing. Charge distribution analysis can comprise procedures such as anion exchange HPLC, or capillary electrophoresis. Detection of impurities includes detecting short mRNA transcripts, RNA-RNA hybrids, and RNA-DNA hybrids. | 2016-02-04 |
20160032274 | RNA-Guided Human Genome Engineering - A method of altering a eukaryotic cell is provided including transfecting the eukaryotic cell with a nucleic acid encoding RNA complementary to genomic DNA of the eukaryotic cell, transfecting the eukaryotic cell with a nucleic acid encoding an enzyme that interacts with the RNA and cleaves the genomic DNA in a site specific manner, wherein the cell expresses the RNA and the enzyme, the RNA binds to complementary genomic DNA and the enzyme cleaves the genomic DNA in a site specific manner. | 2016-02-04 |
20160032275 | NANOPIPETTE DEVICE AND METHOD FOR SUBCELLULAR ANALYSIS - Described herein are devices and methods for extracting cellular material from living cells and then depositing them into to a receptacle in a nanoliter scale. Using a nanopipette integrated into a scanning ion conductance microscope (SICM), extraction of mitochondrial DNA from human BJ fibroblasts and Green Fluorescent Protein (GFP) transcripts from HeLa/GFP cells was achieved with minimal disruption to the cellular milieu and without chemical treatment prior to obtaining the isolated sample. Success of the extraction was confirmed by fluorescence microscopy and PCR analysis of the extracted material. The method and apparatus may be applied to many different cell types and intracellular targets, allowing not only single cell analysis, but single subcellular compartment analysis of materials extracted in their native state. | 2016-02-04 |
20160032276 | SYSTEMS AND METHODS FOR ISOLATING NUCLEIC ACIDS - The present disclosure relates to systems and methods for nucleic acid isolation. In particular, the present disclosure provides systems and methods for purifying nucleic acids for downstream applications. | 2016-02-04 |
20160032277 | Isolating Circulating microRNA (miRNA) - Methods for isolating circulating small RNAs, e.g., microRNA (miRNA), from plasma samples, e.g., that comprise using an alkaline phenol:chloroform extraction, and methods of use thereof, including for the detection, prognosis, and/or monitoring of disease in a subject. | 2016-02-04 |
20160032278 | SCAFFOLD PROTEINS DERIVED FROM PLANT CYSTATINS - The present invention relates to scaffold proteins derived from plant cystatins and to nucleic acids encoding them. The scaffolds are highly stable and have the ability to display peptides. The scaffolds are particularly well suited for constructing libraries, e.g., in phage display or related systems. The invention also relates to various uses of the scaffolds, including in therapy, diagnosis, environmental and security monitoring, synthetic biology and research, and to cells and cell cultures expressing the scaffold proteins. | 2016-02-04 |
20160032279 | Polypeptide Display Libraries and Methods of Making and Using Thereof - Disclosed herein are expression vectors which display a passenger polypeptide on the outer surface of a biological entity. As disclosed herein the displayed passenger polypeptide is capable of interacting or binding with a given ligand. Also disclosed are methods of making and using the expression vectors. N/C terminal fusion expression vectors and methods of making and using are also disclosed. | 2016-02-04 |
20160032280 | METHODS FOR THE PRODUCTION OF LIBRARIES FOR DIRECTED EVOLUTION - Disclosed herein is an efficient method of generating a library of variants of a sequence of interest, such as may be used in directed evolution, in one embodiment, the method includes an amplification reaction, e.g. error-prone PCR, to generate double-stranded DNA (dsDNA) variants of a sequence of interest, after which one strand of the dsDNA variants may be selectively degraded to produce single-stranded DNA (ssDNA) variants. The ssDNA variants may be hybridized to ssDNA intermediaries, e.g., uracilated circular ssDNA intermediaries, to form heteroduplex DNA, which may be transformed into cells, such as | 2016-02-04 |
20160032281 | FUNCTIONALIZED GRIDS FOR LOCATING AND IMAGING BIOLOGICAL SPECIMENS AND METHODS OF USING THE SAME - A functionalized specimen support for use in charged particle microscopy is provided that includes a specimen support surface configured to support specimens during an interrogation of the specimens with a charged particle microscope, the specimen support surface having functionalized sites, each functionalized site configured to maintain position of a portion of one of the specimens at the functionalized site by way of attachment, attraction, or a combination thereof. | 2016-02-04 |
20160032282 | SINGLE CELL BAR-CODING FOR ANTIBODY DISCOVERY - Provided herein are methods and composition for immune repertoire sequencing and single cell barcoding. The methods and compositions can be used to heavy and light chain antibody sequences originating from a single cell, antibody discovery, disease and immune diagnostics, and low error sequencing. | 2016-02-04 |
20160032283 | RANDOM RNAi LIBRARIES, METHODS OF GENERATING SAME, AND SCREENING METHODS UTILIZING SAME - This invention provides expression vectors for a ribonucleic acid (RNA) molecule comprising a double-stranded region of random sequence, sets and libraries of same, methods of generating same, and methods for identifying an RNA therapeutic or RNA molecule that has an ability to affect a biological parameter, for identifying a drug target for a disease or disorder of interest, and for identifying a variant of an RNA molecule that has an altered ability to affect a biological parameter of interest. | 2016-02-04 |
20160032284 | ORGANIC COMPOSITIONS TO TREAT HSF1-RELATED DISEASES - The present disclosure relates to methods of treating heat shock factor 1 (HSF1)-related diseases such as cancer, autoimmune and viral diseases, using a therapeutically effective amount of a RNAi agent to HSF. | 2016-02-04 |
20160032285 | COMPOSITIONS AND METHODS FOR MODULATING TAU EXPRESSION - Disclosed are methods for modulating splicing of Tau mRNA in an animal with Tau antisense compounds. Also disclosed herein are methods for reducing expression of Tau mRNA and protein in an animal with Tau antisense compounds. Such compounds and methods are useful to treat, prevent, or ameliorate neurodegenerative diseases in an individual in need thereof. Examples of neurodegenerative diseases that can be treated, prevented, and ameliorated with the administration Tau antisense oligonucleotides include Alzheimer's Disease, Fronto-temporal Dementia (FTD), FTDP-17, Progressive Supranuclear Palsy, Chronic Traumatic Encephalopathy, Epilepsy, and Dravet's Syndrome. | 2016-02-04 |
20160032286 | INHIBITORS OF MYH7B AND USES THEREOF - The present invention provides nucleic acid inhibitors of MYH7B and compositions thereof. The present invention also provides methods of treating or preventing a cardiac disorder such as cardiac hypertrophy, myocardial infarction, or heart failure in a subject by administering to the subject an inhibitor of MYH7B. The present invention further provides methods of modulating the activity or expression of β-MHC in cardiac cells of a subject by administering to the subject an inhibitor of MYH7B. | 2016-02-04 |
20160032287 | METHOD OF DERIVING MATURE HEPATOCYTES FROM HUMAN EMBRYONIC STEM CELLS - A method for producing mature hepatocytes having functional hepatic enzyme activity from human pluripotent cells is disclosed. The method includes the step of transferring an external vector comprising the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-122, the DNA sequence coding for a microRNA having the seed sequence of the microRNA miR-let-7c, a microRNA having the seed sequence of the microRNA miR-122, a microRNA having the seed sequence of the microRNA miR-let-7c, or a combination thereof into one or more fetal hepatocytes. The resulting cells differentiate into mature hepatocytes that exhibit functional hepatic enzyme activity, and can be used in drug metabolism and toxicity testing, in the study of viruses that target hepatic tissue, and as therapeutics. | 2016-02-04 |
20160032288 | RNA SEQUENCE-SPECIFIC MEDIATORS OF RNA INTERFERENCE - The present invention relates to a | 2016-02-04 |
20160032289 | Oral Delivery of Therapeutically Effective LNA Oligonucleotides - The invention provides for LNA oligomers, for the treatment of a metabolic or liver disorder, wherein the LNA oligomer is administered orally in a unit dose of less than 50 mgs/kg, wherein the LNA oligomer is administered in the presence of a penetration (permeation) enhancer. | 2016-02-04 |
20160032290 | PHARMACEUTICAL COMPOSITION COMPRISING NANOG SHRNA, AND METHOD OF USING NANOG SHRNA TO TREAT CANCER - The present description relates to an inhibitory RNA molecule, comprising an oligonucleotide that selectively knocks down expression a Nanog pseudogene expressed in many human cancers, a replicating viral vector capable of encoding such inhibitory RNA molecule, pharmaceutical compositions comprising said vector, and methods of treating cancer by administration of said pharmaceutical composition. | 2016-02-04 |
20160032291 | CD7 Receptor Aptamers - Nucleic acid aptamers which bind specifically to or are internalised into cells expressing a CD7 cell-surface receptor are disclosed. The aptamers comprise a nucleic acid molecule having a sequence which is at least 80% identical to 5′-GG-GAGACAAGAAUAAGCAUG-R1-UUCGACAGGAGGCUCACAACAGnynz-3′ (SEQ ID NO: 3). The aptamers can be linked to therapeutic or diagnostic molecules, and can be used to deliver the therapeutic or diagnostic molecules to cells expressing the CD7 receptor. The aptamers can also be used as detection tools. | 2016-02-04 |
20160032292 | Aptamer-Guided Gene Targeting - Compositions and methods for modifying genetic material are provided. One embodiment provides aptamers capable of binding to a site-specific DNA binding moiety to facilitate the exchange of homologous genetic information between a donor molecule and the desired target locus (aptamer-guided gene targeting or AGT). One embodiment provides an oligonucleotide containing a aptamer, preferably a DNA aptamer at the 5′ end. The oligonucleotide also contains a region of homology, also referred to as donor DNA, to a desired nucleic acid, locus, or gene. The DNA binding moiety can be a nucleic acid, a protein, or a complex of proteins. In a preferred embodiment the DNA binding moiety is a homing endonuclease that cuts DNA to facilitate the modification of the DNA by the donor DNA. | 2016-02-04 |
20160032293 | SELECTION OF RNA-APTAMERS AS ANTI-MALARIA AGENTS - The present invention relates to an aptamer or an active fragment thereof raised against the semi-conserved duffy binding ligand domain 1α, DBL1α, region of the | 2016-02-04 |
20160032294 | CHLOROGLOEOPSIS SP. HOST CELL FOR PRODUCING ETHANOL AND METHOD FOR PRODUCING ETHANOL USING THE SAME - One embodiment of the invention provides a genetically enhanced | 2016-02-04 |
20160032295 | METHODS, COMPOSITIONS AND KITS FOR A ONE-STEP DNA CLONING SYSTEM - Methods and kits for joining three or more polynucleotides to form a product polynucleotide are provided. Such a method includes forming a reaction mixture comprising (i) a vector fragment whose ends have four base overhangs resulting from cleavage of a vector with a first type IIs enzyme, (ii) a first insert nucleic acid with a four base overhang at one end resulting from cleavage by the first type IIs enzyme and a three base overhang at the other end resulting from cleavage by the second type IIs enzyme; and (iii) a second insert nucleic acid with a four base overhang at one end resulting from cleavage by the first type IIs enzyme and a three base overhang at the other end resulting from cleavage by the second type IIs enzyme, and (iv) a ligase. The four base overhangs of the vector ligate with the four base overhangs of the first and second inserts and the three base overhangs of the first and second inserts ligate with each other or three base overhangs of a spacer nucleic acid resulting from cleavage with the second type IIs enzyme to form a product polynucleotide in which the first and second insert nucleic acids are joined to the vector fragment. | 2016-02-04 |
20160032296 | POLYNUCLEOTIDE SEQUENCES FROM RHODOSPORIDIUM AND RHODOTORULA AND USE THEREOF - The present invention relates to the application of isolated promoters and synthetic constructs for efficient production of genetically modified cells in a species selected from the Pucciniomycotina and Ustilaginomycotina subphyla, in particular, species selected from the | 2016-02-04 |
20160032297 | METHODS FOR THE IDENTIFICATION OF VARIANT RECOGNITION SITES FOR RARE-CUTTING ENGINEERED DOUBLE-STRAND-BREAK-INDUCING AGENTS AND COMPOSITIONS AND USES THEREOF - Methods for the identification of variant recognition sites for rare cutting engineered double strand break inducing agents and compositions thereof are provided. Further provided are nucleic acid constructs, yeast, plants, plant cells, explants, seeds and grain having the of variant recognition sites. Various methods of identifying variant recognition sites with increased substrate activity for a rare cutting engineered double strand break inducing agents are provided. | 2016-02-04 |
20160032298 | REGULATING ALKALOIDS - MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in | 2016-02-04 |
20160032299 | REGULATING ALKALOIDS - MPO1 and MPO2 can be regulated for either decreasing or increasing alkaloid levels in plants, in particular in | 2016-02-04 |
20160032300 | Methods for Producing Hybrid Seed - This invention provides methods for producing a non-natural hybrid seed. Also disclosed are specific miRNAs and miRNA recognition sites useful for conferring inducible sterility on a crop plant, and recombinant DNA construct including such exogenous miRNA recognition sites. | 2016-02-04 |
20160032301 | GUARD CELL EXPRESSION CASSETTES COMPOSITIONS AND METHODS OF USE THEREOF - The invention is directed to artificial transcription regulating polynucleotides that confer guard cell regulated expression and methods of use thereof. The present invention further provides methods of using the transcription regulating polynucleotides and plants and plant parts thereof comprising the transcription regulating polynucleotides. In agricultural biotechnology, plants can be modified according to one's needs. One way to accomplish this is by using modern genetic engineering techniques. For example, by introducing a gene of interest into a plant, the plant can be specifically modified to express a desirable phenotypic trait. | 2016-02-04 |
20160032302 | NOVEL GENE IbENOD93 AND TRANSGENIC PLANTS USING THE SAME - The present invention relates to a novel gene, IbENOD93, and transgenic plants using the same. More specifically, the present invention provides an IbENOD93 gene, an open reading frame (ORF) of the IbENOD93 gene, a recombinant vector comprising the gene or the ORF, and a transformant transformed with the vector. Moreover, the present invention provides a composition for enhancing root thickening growth and promoting maturation in a plant having storage root(s). Furthermore, the present invention provides a method for producing a transgenic plant having storage root(s) with enhanced thickening growth, and a method for regulating or enhancing root thickening growth and maturation in a plant having storage root(s). According to the present invention, it is possible to promote the thickening growth of storage roots as well as the growth of aerial part. Therefore, the present invention can be effectively used to promote the thickening growth of storage roots of high-value root crops such as ginseng, sweet potato, etc. and to produce early-maturing transgenic plants. | 2016-02-04 |
20160032303 | PATHOGEN-INDUCIBLE PROMOTERS AND THEIR USE IN ENHANCING THE DISEASE RESISTANCE OF PLANTS - Methods for producing pathogen-inducible promoters for the expression of genes in plants are provided. The pathogen-inducible promoters are inducible by one, two, three, or more plant pathogens. Methods for producing R genes that are inducible in a plant by more than one plant pathogen are further provided. Additionally, provided are R genes and other nucleic acid molecules comprising the pathogen-inducible promoters and that are made by such methods as well as plants, plant parts, plant cells, seeds, and non-human host cells comprising the R genes and other nucleic acid molecules | 2016-02-04 |
20160032304 | SLM1, A SUPPRESSOR OF LESION MIMIC PHENOTYPES - Methods and compositions for modulating Slm1 are provided. Methods are provided for modulating the expression of Slm1 in a host plant or plant cell to modulate agronomic characteristics. | 2016-02-04 |
20160032305 | PLANT CYTOCHROME P450 - This disclosure relates to the isolation and sequencing of nucleic acid molecules that encode cytochrome P450 polypeptides from a | 2016-02-04 |
20160032306 | IDENTIFICATION AND USE OF TOMATO GENES CONTROLLING SALT/DROUGHT TOLERANCE AND FRUIT SWEETNESS - The present invention provides the identification and use of EGTom1 and/or EGTom2, homologs of EGTom1 and/or EGTom2, orthologs of EGTom1 and/or EGTom2, paralogs of EGTom1 and/or EGTom2, and fragments and variations thereof for altering salt tolerance, drought tolerance and/or sugar content of fruit (sweetness) in plants. The invention relates to the identification and use of nucleic acid sequences for salt/drought tolerance and fruit sweetness in plants. | 2016-02-04 |
20160032307 | MODIFYING THE FATTY ACID PROFILE OF CAMELINA SATIVA OIL - The present disclosure provides methods and compositions for modifying fatty acids in | 2016-02-04 |
20160032308 | Papaver Somniferum Strain with High Concentration of Thebaine - The present invention is directed to an improved poppy straw, concentrate of poppy straw and opium of | 2016-02-04 |
20160032309 | NUCLEIC ACID IMPARTING HIGH-YIELDING PROPERTY TO PLANT, METHOD FOR PRODUCING TRANSGENIC PLANT WITH INCREASED YIELD, AND METHOD FOR INCREASING PLANT YIELD - An object of the present invention is to provide nucleic acids capable of imparting high-yielding ability to plants. Another object of the present invention is to use such nucleic acids to produce transgenic plants at increased yield, as well as to provide methods for increasing the yield of plants. By introducing into a plant a construct in which a promoter of a pseudo-response regulator gene in | 2016-02-04 |
20160032310 | METHODS AND COMPOSITIONS FOR OBTAINING USEFUL EPIGENETIC TRAITS - The present invention provides methods for obtaining plants that exhibit useful traits by expression of a recombinant DNA methyltransferase in progenitor plants. Methods for identifying genetic loci that provide for useful traits in plants and plants produced with those loci are also provided. In addition, plants that exhibit the useful traits, parts of the plants including seeds, and products of the plants are provided as well as methods of using the plants. Recombinant DNA vectors and transgenic plants comprising those vectors that express a recombinant DNA methyltransferase are also provided. | 2016-02-04 |
20160032311 | AGROBACTERIUM RHIZOGENES TRANSFORMATION AND EXPRESSION OF ROL GENES IN KALANCHOE - The present disclosure embraces | 2016-02-04 |
20160032312 | METHOD OF INCREASING PHOTOSYNTHESIS AND YIELD OF PLANTS - The present invention pertains to a method for increasing the photosynthesis and yield/growth of plants, and a transgenic plant which is used in the method. | 2016-02-04 |
20160032313 | METHODS OF INCREASING RESISTANCE OF CROP PLANTS TO HEAT STRESS AND SELECTING CROP PLANTS WITH INCREASED RESISTANCE TO HEAT STRESS - Methods of increasing the resistance of a crop plant to heat stress and in particular methods of improving the grain yield and quality of crop plants grown under heat stress in the form of increased minimal temperatures are provided. The methods include selecting plants with increased expression of HYR and growing these plants in regions expected to experience minimal temperatures above 25° C. during the growing season. Methods of screening plants for increased resistance to heat stress and methods of producing grain in regions having minimal temperatures of 25° C. or more are also provided. | 2016-02-04 |
20160032314 | CONTROLLING FUNGAL PATHOGENS BY DISABLING THEIR SMALL RNA PATHWAYS USING RNAi-BASED STRATEGY - The present invention relates to pathogen-resistant plants. In one aspect, plants comprising a heterologous expression cassette are provided, wherein the expression cassette comprises a polynucleotide that inhibits expression of a fungal pathogen dicer-like (DCL) gene and wherein the plant has increased resistance to a fungal pathogen compared to a control plant lacking the expression cassette. Methods of making and cultivating pathogen-resistant plants are also provided. | 2016-02-04 |
20160032315 | SOYBEAN RESISTANT TO CYST NEMATODES - A transgenic soybean resistant to soybean cyst nematode (SCN), or parts thereof, including an artificial DNA construct encoding a serine hydroxymethyltransferase protein (e.g., GmSHMT). Also provided are GmSHMT alleles containing mutations R130P and Y358N along with research and breeding methods and compositions including such. | 2016-02-04 |
20160032316 | Purification and Purity Assessment of RNA Molecules Synthesized with Modified Nucleosides - This invention provides purified preparations of an RNA, oligoribonucleotide, or polyribonucleotide comprising a modified nucleoside, and methods of assessing purity of purified preparations of an RNA, oligoribonucleotide, or polyribonucleotide comprising a modified nucleoside. | 2016-02-04 |
20160032317 | COMPOSITIONS AND METHODS FOR REPROGRAMMING HEMATOPOIETIC STEM CELL LINEAGES - Provided herein are compositions, methods, and kits for hematopoietic stem cell induction or for reprogramming cells to the multipotent state of hematopoietic stem cells. In some embodiments, the compositions comprise at least one HSC inducing factor. Such compositions, methods and kits can be used for inducing hematopoietic stem cells in vitro, ex vivo, or in vivo, as described herein, and these induced hematopoietic stem cells can be used in regenerative medicine applications and therapies. | 2016-02-04 |
20160032318 | INSULATOR TO IMPROVE GENE TRANSFER VECTORS - The present invention solves the problem of providing more efficient barrier insulators to avoid vector silencing and to increase expression in the setting of gene transfer vectors, more particularly in the setting of gene transfer retroviral vectors. In this sense, the authors of the present invention have developed an improved insulator element, namely element IS2, which comprises the following combination of nucleic acid molecules, namely nucleic acid molecule HS4-650 bp as shown in SEQ ID No 2 and a synthetic S/MAR nucleic acid molecule containing 5 M/SARs recognition signatures (MRS) as shown in SEQ ID no 1. | 2016-02-04 |
20160032319 | VECTORS COMPRISING STUFFER/FILLER POLYNUCLEOTIDE SEQUENCES AND METHODS OF USE - Recombinant viral vectors such as AAV vectors designed with expression cassettes that approach the natural packaging capacity of the virus, such as AAV are provided. The recombinant viral vectors reduce residual plasmid DNA impurities. | 2016-02-04 |
20160032320 | NON-LIPOSOMAL SYSTEMS FOR NUCLEIC ACID DELIVERY - The present invention provides novel, stable lipid particles having a non-lamellar structure and comprising one or more active agents or therapeutic agents, methods of making such lipid particles, and methods of delivering and/or administering such lipid particles. More particularly, the present invention provides stable nucleic acid-lipid particles (SNALP) that have a non-lamellar structure and that comprise a nucleic acid (such as one or more interfering RNA), methods of making the SNALP, and methods of delivering and/or administering the SNALP. | 2016-02-04 |
20160032321 | METHODS OF NUCLEASE-BASED GENETIC ENGINEERING - The present invention relates to genetic techniques employing the direct ligatation of an external DNA fragment generated in situ by the same ZFNs that target the genome. ObLiGaRe, i.e., the obligated ligation-gated recombination, is a new method for genetic engineering using custom designed nucleases, and a strategy of site-specific gene insertion utilizing the NHEJ pathway. It applies a similar logic to the one used in unidirectional loxP sites(Oberdoerffer et al., 2003) but maintains all the advantages and flexibility of CDNs. | 2016-02-04 |
20160032322 | Microvesicles - A method of producing a population of differentiated cells comprising: a) inducing differentiation in a first population of cells by applying an inducer to said cells, b) harvesting microvesicles produced from first population of cells, and c) inducing differentiation in a second population of cells by applying said microvesicles or a derivative thereof to said second population of cells wherein, said first population of cells is autologous to said second population of cells and wherein the inducer applied to said first population of cells is not present in said second population of cells or is only present in trace amounts. Also related methods of producing microvesicles, methods of medical and/or cosmetic treatment and related products and uses. | 2016-02-04 |
20160032323 | RECOMBINANT MICROORGANISMS FOR ENHANCED PRODUCTION OF MEVALONATE, ISOPRENE, AND ISOPRENOIDS - The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, (c) acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms | 2016-02-04 |
20160032324 | Process for producing fermentation products and fermentation medium compositions therefor - Fermentation products (e.g., bio-products such as hydrocarbons and other organic compounds) are produced from biomass or gases through digestion and fermentation under conditions that thermodynamically favor production of the fermentation products. | 2016-02-04 |
20160032325 | MODIFIED MICROORGANISMS AND METHODS OF MAKING BUTADIENE USING SAME - The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom. | 2016-02-04 |
20160032326 | Production of Alkenes by Combined Enzymatic Conversion of 3-Hydroxyalkanoic Acids - The present invention relates to a method for generating alkenes through a biological process. More specifically, the invention relates to a method for producing alkenes (for example propylene, ethylene, 1-butylene, isobutylene or isoamylene) from molecules of the 3-hydroxyalkanoate type. | 2016-02-04 |
20160032327 | PROCESS FOR CULTURING DEINOCOCCUS BACTERIA - The invention relates to processes of culture of a | 2016-02-04 |
20160032328 | METHODS AND COMPOSITIONS FOR ENHANCED PRODUCTION OF FATTY ALDEHYDES AND FATTY ALCOHOLS - The invention relates to the use of EntD polypeptides, polynucleotides encoding the same, and homologues thereof to enhance the production of fatty aldehydes and fatty alcohols in a host cell. | 2016-02-04 |
20160032329 | USE OF BACTERIA FOR THE PRODUCTION OF BIOENERGY - The present invention relates to composition and methods of producing bioenergy. More specifically, the invention relates to the use of bacterium of the genus | 2016-02-04 |
20160032330 | P-450-CATALYZED ENANTIOSELECTIVE CYCLOPROPANATION OF ELECTRON-DEFICIENT OLEFINS - The present invention pertains to the use of engineered variants of enzyme CYP102A, also known as P450-BM3, for cyclopropanation of olefins containing electron-withdrawing groups. One exemplary enzyme variant, referred to as BM3-HStar, contains five mutations away from wild-type P450-BM3, and demonstrates high activity towards cyclopropanation of olefinic substrates using ethyldiazoacetate (EDA) and other carbene transfer reagents. Products of these reactions are potential precursors of levomilnacipran derivatives, a class of compounds that have been shown to be selective inhibitors of monoamine transporters. In addition, cyclopropanation reactions with the P450-BM3 enzyme variants of the invention can be conducted in whole cells expressing the enzyme variants and can proceed under aerobic conditions. | 2016-02-04 |
20160032331 | Methods and Systems For Biological Coal-to-Biofuels and Bioproducts - Methods and systems for the biological conversion of pretreated or solubilized coal or waste coal into biofuels. Coal ( | 2016-02-04 |
20160032332 | VARIANT THIOESTERASES AND METHODS OF USE - The present invention relates to variant thioesterases and their use in plants, e.g., to increase enzymatic activity and to promote increased production of mid-chain length fatty acids (e.g., 8 to 14 carbons) and at desired ratios. Further disclosed herein are methods of manufacturing renewable chemicals through the manufacture of novel triglyceride oils followed by chemical modification of the oils. Oils containing fatty acid chain lengths of C8, C10, C12 or C14 are also disclosed and are useful as feedstocks in the methods described herein. | 2016-02-04 |
20160032333 | METHOD FOR GROWING ALGAE - A method for converting flue gas into useable bio-oil based compounds using algae includes mixing flue gas with water, infusing the mixture with a lipid trigger and nutrients, distributing the mixture into processing cells, exposing the mixture to light, promoting the algae to the top of the processing cells for harvest, skimming the algae from the surface of the water, and extracting bio-oil from the algae. | 2016-02-04 |
20160032334 | METHODS FOR THE SYNTHESIS OF CHIRAL KYNURENINE COMPOUNDS - Provided are methods for synthesizing compounds, including chiral kynurenine compounds. The methods are suitable for large-scale manufacture and produce the chiral kynurenines compounds in high chemical purity and high chiral purity. | 2016-02-04 |
20160032335 | (R)- SELECTIVE AMINATION - The present invention relates to a method for the enzymatic synthesis of enantiomerically enriched (R)-amines of general formula [1][c] | 2016-02-04 |
20160032336 | Polypeptides Having Xylanase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having xylanase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2016-02-04 |
20160032337 | PROCESS FOR THE PRODUCTION OF HYALURONIC ACID IN ESCHERICHIA COLI OR BACILLUS MEGATERIUM - A method of producing hyaluronic acid (HA) in | 2016-02-04 |
20160032338 | METHOD OF USING ALPHA-AMYLASE FROM ASPERGILLUS FUMIGATUS AND ISOAMYLASE FOR SACCHARIFICATION - A fungal alpha-amylase is provided from | 2016-02-04 |
20160032339 | CELLULOSIC ENZYME RECYCLING FROM SEPARATION OF SACCHARIFIED BIOMASS - The present disclosure provides methods for generating sugars from a cellulosic biomass. The methods combine treatment of the biomass using a high-shear milling device and saccharification of the biomass to partially hydrolyze the biomass. The biomass can be saccharified either after or simultaneously with the high-shear milling treatement. The partially hydrolyzed biomass is then separated into a solids stream with saccharification enzymes, and a liquid stream with sugars. The solids stream and associated enzymes are further incubated under saccharification conditions to produce additional sugars, or are recycled and added to fresh biomass, which is saccharified under high-shear milling conditions. The methods result in improved conversion of cellulosic biomass to glucose. | 2016-02-04 |
20160032340 | Method and Compositions for Improved Lignocellulosic Material Hydrolysis - A method of digesting a lignocellulosic material is disclosed. In one embodiment, the method comprises the step of exposing the material to an effective amount of | 2016-02-04 |
20160032341 | PROCESSING BIOMASS AND PETROLEUM CONTAINING MATERIALS - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation. | 2016-02-04 |
20160032342 | Mutant Endoglycoceramidases with Enhanced Synthetic Activity - The present invention relates to a novel endoglycoceramidase whose hydrolytic activity has been substantially reduced or eliminated, such that the enzyme is useful for synthesis of glycolipids from a monosaccharide or oligosaccharide and a ceramide. More specifically, the endoglycoceramidase is a mutant version of a naturally occurring endoglycoceramidase, preferably comprising a mutation within the active site or the nucleophilic site of the enzyme and more preferably comprising a substitution mutation of the Glu residue within the active site or the nucleophilic site. Also disclosed are a method for generating the mutant endoglycoceramidase and a method for enzymatically synthesizing glycolipids using this mutant enzyme. | 2016-02-04 |
20160032343 | METHODS FOR INCREASING MANNOSE CONTENT OF RECOMBINANT PROTEINS - The present invention relates to methods of modulating the mannose content of recombinant proteins. | 2016-02-04 |
20160032344 | POLYSIALIC ACID, BLOOD GROUP ANTIGENS AND GLYCOPROTEIN EXPRESSION IN PROKARYOTES - The invention described herein generally relates to glycoengineering host cells for the production of glycoproteins for therapeutic use. Host cells are modified to express biosynthetic glycosylation pathways. Novel prokaryotic host cells are engineered to produce N-linked glycoproteins wherein the glycoproteins comprise polysialic acid or blood group antigens. | 2016-02-04 |
20160032345 | METHOD FOR PRODUCING SOLUBLE RECOMBINANT INTERFERON PROTEIN WITHOUT DENATURING - The present invention relates to the field of recombinant protein production in bacterial hosts. It further relates to extraction of soluble, active recombinant protein from an insoluble fraction without the use of denaturation and without the need for a refolding step. In particular, the present invention relates to a production process for obtaining high levels a soluble recombinant Type 1 interferon protein from a bacterial host. | 2016-02-04 |
20160032346 | Sortase-mediated protein purification and ligation - The invention relates to a sortase-mediated protein purification and ligation. Specifically, the invention relates to a technique that links protein expression/purification with conjugation to therapeutic agents, imaging agents, or linkers. | 2016-02-04 |
20160032347 | NOVEL METHOD AND DEVICE FOR WHOLE-CELL BACTERIAL BIO-CAPACITOR CHIP FOR DETECTING CELLULAR STRESS INDUCED BY TOXIC CHEMICALS - The present invention is directed to methods and a bio-capacitor sensing device for the detection of toxic chemicals using bacteria. The sensing platform comprises gold interdigitated capacitor with a defined geometry, a layer of carboxy-CNTs immobilized with viable | 2016-02-04 |
20160032348 | SEPARATION DEVICE FOR USE IN THE SEPARATION, CHARACTERIZATION AND/OR IDENTIFICATION OF MICROORGANISMS - The present invention is directed to a separation device or container that can be used in the separation, isolation or pelleting of microorganisms from a test samples known to contain or suspected of containing said microorganisms. Subsequently, the separated, isolated or pelleted microorganism sample can undergo one or more interrogation steps to provide measurements useful for the characterization and/or identification of microorganism. In one aspect of the present invention, the interrogation steps can occur in situ in the separation device or container described herein. | 2016-02-04 |
20160032349 | MITRECIN A POLYPEPTIDE WITH ANTIMICROBIAL ACTIVITY - The present invention provides a Mitrecin A polypeptide useful in prevention and treatment of one or more bacteria. Also provided is a method to kill or prevent growth of one or more bacteria comprising contacting the one or more bacteria with a Mitrecin A polypeptide. The target bacteria can be selected from the group consisting of a Gram-positive bacterium, a Gram-negative bacterium, or both. In one embodiment, the present invention is drawn to a polynucleotide encoding a Mitrecin A polypeptide, a vector comprising the polynucleotide, a host cell comprising the polynucleotide, or a composition comprising the Mitrecin A polypeptide, the polynucleotide, the vector, or the host cell. | 2016-02-04 |
20160032350 | Microfluidic Microbe Detection and Isolation - A method and microfluidic device useful for isolating microbes from a blood sample which includes introducing the blood sample into the sample inlet of a spiral microfluidic device; and introducing a second fluid into the sheath inlet of the microfluidic device, wherein the spiral channel terminates in a microbe outlet and a waste outlet, and wherein the spiral channel includes a length, height, and a width that define an aspect ratio adapted to isolate any microbes present in the sample along a first portion of the spiral channel terminating at the microbe outlet, and to isolate red blood cells and leukocytes along a second portion of the spiral channel terminating at the waste outlet; and collecting the microbes from the microbe outlet, thereby isolating the microbes. | 2016-02-04 |
20160032351 | METHODS AND APPARATUS FOR HANDLING MICROBIAL SAMPLES - This invention pertains to the general field of microbiology, and more specifically to transfer, inoculation and/or streaking of micro-organisms, e.g. for the purpose of obtaining individual colonies. Provided is a method for transferring a microbial sample from a first carrier to a second carrier, comprising the steps of:
| 2016-02-04 |
20160032352 | Composition, Device, and Method for Biological Air Sampling - The present invention generally relates to air sampling of biological compounds. Specifically, the present invention relates to a device and method for sampling the ambient air for detecting microbial propagules, microbial propagules being any spore, vegetative cell, or virion of microbiological origin including all bacteria, fungi, viruses, protozoans, molds, slime molds, chlamydospores, hyphae, and cysts. | 2016-02-04 |