| 05th week of 2010 patent applcation highlights part 49 |
| Patent application number | Title | Published |
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| 20100028894 | Photographic Determination of Analytes - The present invention refers to a detection method for analytes using the principle of black-and-white photography and to reagent kits for performing the method, furthermore applied this new technology to detect a biologically relevant sequence in the nanomolar range (femtomoles) in an application circumventing the necessity of a PCR. There are still numerous ways to optimize this methodology that is suitable for a large variety of applications in the genomic diagnostics and proteomics areas. | 2010-02-04 |
| 20100028895 | METHOD FOR DETERMINING THE STABILITY OF ORGANIC METHYLENEAMINES IN THE PRESENCE OF SEMICARBAZIDE-SENSITIVE AMINE OXIDASE - The present invention provides methods for determining the stability of methyleneamine, methyleneamine-like compounds or compounds containing an methyleneamine moiety in the presence of semicarbazide-sensitive amine oxidase (SSAO) or a biological sample containing SSAO activity. The disclosed methods may be configured in an assay format for high throughput screening applications. | 2010-02-04 |
| 20100028896 | ROTARY EXTRACTION CONTAINER AND METHOD OF IDENTIFYING CELL SPECIES, METHOD OF DETECTING GENE, AND AUTOMATIC NUCLEIC ACID EXTRACTOR USING THE SAME - Disclosed is a rotary extraction container enabling to safely and simply perform extraction and separation of a target substance from a sample containing plural substances. Specifically, there is disclosed a rotary extraction container enabling to simply perform extraction and separation of a nucleic acid from a biological sample or from a bio-derived sample without any risk of infection, contamination or the like, which has conventionally required cumbersome operations and a large, expensive apparatus. Further, there is disclosed a method of identifying a cell species a method of detecting a gene and an automatic nucleic acid extractor using the same. The foregoing rotary extraction container, which is a rotary extraction container to extract a target substance from a sample comprises a cylindrical container section, a rotating section and a cover section, and a solution or solid contained in any one of the small chambers of the cylindrical container section is allowed to transfer to another of the small chambers by an operation including rotation of the rotating section and the target substance is extracted from the sample by such an operation including the transfer. | 2010-02-04 |
| 20100028897 | APPARATUS AND METHOD FOR EXAMINING BIOPOLYMER - A biopolymer examining apparatus includes a capsule-forming unit configured to form a capsule by sealing a target biopolymer and a reagent with a capsule film, a transferring unit configured to transfer the capsule, an amplification reaction unit configured to amplify the target biopolymer while having the target biopolymer enclosed in the capsule, and a detecting unit configured to detect the amplified target biopolymer while having the target biopolymer enclosed in the capsule. | 2010-02-04 |
| 20100028898 | METHOD OF IDENTIFYING A TARGET ANALYTE USING PHOTONIC CRYSTAL RESONATORS, AND RELATED DEVICE - A method of identifying target analytes in a sample, particularly a biological sample, comprising the steps of putting a plurality of target analytes, bound to a common luminescent marker, in contact with a plurality of molecular probes immobilized on a support, each of said molecular probes being capable of complementary binding to a respective target analyte, if said target analyte is present in the sample, providing to said support an excitation radiation and detecting an emission radiation coming from said support as a result of at least one complementary binding event, characterized by the fact that:
| 2010-02-04 |
| 20100028899 | CELL CYCLE PHASE MARKERS - The present invention relates to polypeptide and nucleic acids constructs which are useful for determining the cell cycle status of a mammalian cell. Host cells transfected with these nucleic acid constructs can be used to determine the effects that test agents have upon the mammalian cell cycle. | 2010-02-04 |
| 20100028900 | SCREENING METHODS AND SEQUENCES RELATING THERETO - Disclosed are screening methods and sequences related thereto. Disclosed are methods for detecting mutations in the MYH gene of an individual. Also disclosed are methods of genotyping and methods of predicting for an individual the likelihood of developing certain cancers, such as colorectal cancer. | 2010-02-04 |
| 20100028901 | METHOD FOR DIAGNOSING IN VITRO OR EX VIVO PSYCHIATRIC DISORDERS AND/OR INTESTINAL DYSBIOSES - The invention relates to the use of at least one of the following Formula (I) compounds: | 2010-02-04 |
| 20100028902 | LIVING CELL FORCE SENSORS AND METHODS OF USING SAME - Disclosed herein are materials and methods for the efficient and universal fabrication of microcantilevers terminated with living cells. Methods disclosed describe the passive attachment of cells to microcantilevers that represent cells in suspension comprising living cells attached thereto via association with a hydrophobic layer. Also, disclosed are efficient methods for seeding single and multiple cells to cantilevers that represent isolated adherent cells and tissue constructs of tunable confluency. | 2010-02-04 |
| 20100028903 | METHOD AND DEVICE FOR EXAMINING THE ATTACHMENT OR DETACHMENT OF LIVING OR DEAD CELLS OR CELL-LIKE PARTICLES OR OTHER SURFACE ACCUMULATIONS ON SURFACES BY MEANS OF PLASMON RESONANCE AND USE OF SAID METHOD AND SAID DEVICE - The invention relates to the examination of the attachment or detachment of living or dead cells, or cell-like particles, or other surface accumulations on surfaces, by means of plasmon resonance. The reflected light is evaluated such that two or more intensity minima of the reflected light occurring within a time frame are registered in a time dependant manner for different incident angles. The registered measurement signals reflect the respective amount of the surface accumulation. In case two intensity minima have occurred at the same time, valuable information about particular processes regarding the surface accumulation, for example regarding the adhesion of cells, can be obtained. Said information is of particular importance in the field of medicine/biology. | 2010-02-04 |
| 20100028904 | WAY TO OBTAIN HIGH EXPRESSION CLONES OF MAMMALIAN CELLS USING A METHYLCELLULOSE WITH FLUORESCENT PROTEIN A OR G AND FLUORESCENT SCREENING METHOD - The invention provides a genetic screening method for identifying a transfected cell expressing the polypeptide of interest. The methods allows for high throughput screening of recombinant cells for elevated levels of expression of the polypeptide of interest using methylcellulose comprising fluorescent protein A or G to improve detection and cloning. The invention also provides capture media, formulations and methods of making and using thereof. | 2010-02-04 |
| 20100028905 | CHARACTERIZATION OF GRP94-LIGAND INTERACTIONS AND PURIFICATION, SCREENING, AND THERAPEUTIC METHODS RELATING THERETO - The presently disclosed subject matter discloses characterization of interactions between ligands and Hsp90 proteins, including GRP94, wherein ligand binding to the N-terminal nucleotide binding domain of GRP94 elicits a conformational change that converts the GRP94 from an inactive to an active conformation, and wherein the chaperone and peptide-binding activities of the GRP94 are markedly stimulated. Also disclosed are purification, screening, and therapeutic methods pertaining to the biological activity of GRP94, and in some instances HSP90, based upon the characterization of ligand interactions of Hsp90 peptide-binding proteins, including GRP94. | 2010-02-04 |
| 20100028906 | REDUCING THE RISK OF HUMAN ANTI-HUMAN ANTIBODIES THROUGH V GENE MANIPULATION - The present embodiments relate to methods of identifying and creating human or humanized antibodies that possess a reduced risk of inducing a Human Anti-Human Antibody (HAHA) response when they are applied to a human host. Other methods are directed to predicting the likelihood of a HAHA response occurring. Methods for screening for anti-HAHA compounds are also included. Methods for determining if various conditions for administering an antibody to a subject enhance or suppress a HAHA response are also included. | 2010-02-04 |
| 20100028907 | Novel Antigens and Antibodies Associated to Pancreatic Ductal Adenocarcinoma - The present invention provides novel human protein antigens and related antibodies that have been identified as being specifically expressed in association to human Pancreatic Ductal Adenocarcinoma (PDA). In particular, novel phosphorylated isoforms of alpha-enolase have been identified in transformed cell lines of pancreatic origin and antibodies binding such isoforms are specifically present in the sera of PDA patients. These proteins and antibodies can be useful for the diagnosis and the treatment of PDA and other cancers having common molecular features. | 2010-02-04 |
| 20100028908 | NOVEL DETECTION SYSTEM - The present invention relates to methods of making nanoarrays for use in detecting species formed on the surface of the array using SE(R)RS. The methods can involve nanolithographic printing of a compound by dip pen nanolithographic printing. A SE(R)RS substrate can be used for the array and which can be selected from surfaces roughened by the oxidation-reduction cycle (ORC), island films, colloidal nanoparticles and surface-confined nanostructures. A coating or intermediate layer, such as a layer formed of nitrocellulose, can be provided between the compound and the SE(R)RS substrate. There are also provided arrays themselves and methods of using such arrays. | 2010-02-04 |
| 20100028909 | In Vitro Method for Diagnosing and Monitoring Metastasized Bladder Cancer Using the Determination of MMP-7 in the Circulation of Patients - The present invention relates to the use in diagnostic in vitro methods for the detection, early detection, monitoring and/or prognosis of metastatic bladder cancer in human patients of matrix metalloproteinase 7 (MMP-7) and/or its precursors and fragments with MMP-7 immunoreactivity that can be measured in blood, serum or plasma samples from the patient. | 2010-02-04 |
| 20100028910 | ACTIVATED PROTEIN C VARIANTS WITH NORMAL CYTOPROTECTIVE ACTIVITY BUT REDUCED ANTICOAGULANT ACTIVITY - Variants (mutants) of recombinant activated protein C (APC) or recombinant protein C (prodrug, capable of being converted to APC) that have substantial reductions in anticoagulant activity but that retain normal levels of anti-apoptotic activity are provided. Two examples of such recombinant APC mutants are KKK191-193AAA-APC and RR229/230AA-APC. APC variants and prodrugs of the invention have the desirable property of being cytoprotective (anti-apoptotic effects), while having significantly reduced risk of bleeding. The invention also provides a method of using the APC variants or prodrugs of the invention to treat subjects who will benefit from APC's cytoprotective activities that are independent of APC's anticoagulant activity. These subjects include patients at risk of damage to blood vessels or tissue in various organs caused, at least in part, by apopotosis. At risk patients include, for example, those suffering (severe) sepsis, ischemia/reperfusion injury, ischemic stroke, acute myocardial infarction, acute or chronic neurodegenerative diseases, or those undergoing organ transplantation or chemotherapy, among other conditions. Methods of screening for variants of recombinant protein C or APC that are useful in accordance with the invention are also provided. | 2010-02-04 |
| 20100028911 | MULTICOLOR REAGENTS CONTAINING COMPENSATION CONTROLS - The present invention provides multicolor reagent formulations containing in a single container both fluorescently labeled detection reagents and single-color compensation control reagents, wherein the compensation control reagents consist of reagent-capture particles bound to a fluorescently labeled detection reagent included in the multicolor reagent formulation. The multicolor reagent formulations of the present invention simplify manufacture and commercial distribution of multicolor reagent kits. | 2010-02-04 |
| 20100028912 | IMAGING USING RADIOACTIVE MONOCATIONS IN COMBINATION WITH A RECEPTOR BINDING LIGAND THAT STIMULATES THE INFLUX OF CATIONS - A novel method of imaging, comprising administration of a radioactive mono-cation, such as, for example, | 2010-02-04 |
| 20100028913 | NOVEL CARBOHYDRATE FROM HUMAN CELLS AND METHODS FOR ANALYSIS AND MODIFICATION THEREOF - The invention describes reagents and methods for specific binders to glycan structures of stem cells. Furthermore the invention is directed to screening of additional binding reagents against specific glycan epitopes on the surfaces of the stem cells. The preferred binders of the glycans structures includes proteins such as enzymes, lectins and antibodies. | 2010-02-04 |
| 20100028914 | ASSESSING LUNG NODULES - This document provides methods and materials related to distinguishing malignant solitary pulmonary nodules from benign solitary pulmonary nodules in mammals (e.g., humans). | 2010-02-04 |
| 20100028915 | CIRCULATING TUMOR CELL ASSAY - Methods for the detection, enumeration and analysis of circulating tumor cells expressing insulin-like growth factor-1 receptors (IGF-1R) are disclosed. These methods are useful for cancer screening and staging, development of treatment regimens, and for monitoring for treatment responses, cancer recurrence or the like. Test kits that facilitate the detection, enumeration and analysis of such circulating tumor cells are also provided. | 2010-02-04 |
| 20100028916 | Method for the detection of enzymatic reactions - The present invention provides a method for the detection of an enzyme E1 in a liquid sample comprising the steps of: a) providing a complex (Sa-Sb-M), wherein (Sa-Sb) is a substrate S of E1 cleavable into Sa and Sb by E1, and M is a marker linked to Sb, b) incubating the sample with the complex under conditions enabling the cleavage of S into Sa and Sb by E1, c) separating non-cleaved complex (Sa-Sb-M) from the sample, and d) measuring M in the sample. Furthermore, the present invention further provides kits and devices for the detection of an enzyme E1. | 2010-02-04 |
| 20100028917 | A NEUROGLOBIN ENZYME-LINKED IMMUNOSORBENT ASSAY KIT AND THE USE OF IT - The invention discloses a Neuroglobin enzyme-linked immunosorbent assay kit and the use of it. The Neuroglobin enzyme-linked immunosorbent assay kit provided by the invention includes Neuroglobin polyclonal antibody, Neuroglobin monoclonal antibody and enzyme labeled antibody. Neuroglobin monoclonal antibody is produced through culturing hybridoma cells prepared by cell conjugation after inoculating mice with Neuroglobin antigen, whereas Neuroglobin polyclonal antibody is produced from animal antiserum after inoculating with Neuroglobin antigen. | 2010-02-04 |
| 20100028918 | GLUTAMINYL CYCLASE AS A DIAGNOSTIC/PROGNOSTIC INDICATOR FOR NEURODEGENERATIVE DISEASES - A method for predicting, diagnosing and prognosticating a neurodegenerative disease, such as Alzheimer's disease (AD), Mild Cognitive Impairment (MCI) and neurodegeneration in Down's syndrome (NDS) using glutaminyl cyclase (QC) as a diagnostic/prognostic indicator. The use of antibodies binding to QC and kits for performing said diagnostic method are also provided. | 2010-02-04 |
| 20100028919 | METHOD FOR THE EARLY DETECTION OF RENAL INJURY - A method and kit for detecting the immediate or early onset of renal disease and injury, including renal tubular cell injury, utilizing NGAL as an immediate or early on-set biomarker in a sample of blood serum. NGAL is a small secreted polypeptide that is protease resistant and consequently readily detected in the blood serum following renal tubule cell injury. NGAL protein expression is detected predominantly in proximal tubule cells, in a punctuate cytoplasmic distribution reminiscent of a secreted protein. The appearance NGAL in the serum is related to the dose and duration of renal ischemia and nephrotoxemia, and is diagnostic of renal tubule cell injury and renal failure. NGAL detection is also a useful marker for monitoring the nephrotoxic side effects of drugs or other therapeutic agents. | 2010-02-04 |
| 20100028920 | Hydrogen Peroxide Droplet-Based Assays - The present invention relates to providing improved hydrogen peroxide assays, as well as droplet actuators for conducting such assays. The droplet actuators of the invention may be used to conduct droplet-based hydrogen peroxide assays. They may also be associated with detectors for analyzing the results of the hydrogen peroxide assays of the invention. They may be provided as components of systems which control droplet operations and/or detection for conducting the hydrogen peroxide assays. Measurement by the detector may be used to quantify the presence of an analyte in a sample. | 2010-02-04 |
| 20100028921 | RISK STRATIFICATION FOR ACUTE CORONARY SYNDROME BY MEANS OF FRAGMENTS/PARTIAL PEPTIDES OF PROVASOPRESSIN, ESPECIALLY COPEPTIN OR NEUROPHYSIN II - The invention relates to a method for risk stratification for acute coronary syndrome (ACS), in particular acute myocardial infarction (AMI) and angina pectoris (AP), wherein provasopressin (proAVP) or fragments and partial peptides thereof, in particular copeptin or neurophysin II, is determined by an in vitro diagnosis. | 2010-02-04 |
| 20100028922 | IDENTIFICATION AND CHARACTERIZATION OF HCV REPLICON VARIANTS WITH REDUCED SUSCEPTIBILITY TO BENZOFURANS, AND METHODS RELATED THERETO - The present invention provides methods of decreasing the frequency of emergence, decreasing the level of resistance, and delaying the emergence of a treatment-resistant Hepatitis C viral infection, by administering to a subject, either in combination or in series, an inhibitor of the Hepatitis C RNA-dependent RNA polymerase NS5B, e.g., a benzofuran, such as 5-cyclopropyl-2-(4-fluorophenyl)-6-[(2-hydroxyethyl)(methylsulfonyl)amino]-N-methyl-1-benzofuran-3-carboxamide (HCV-796), and at least one additional anti-Hepatitis C agent, e.g., a ribavirin product or an immunomodulator, such as an interferon product. Additionally, the invention relates to methods of monitoring the course of treatment of a Hepatitis C viral infection, methods of monitoring and prognosing a Hepatitis C viral infection, and methods of identifying an individual with a decreased likelihood of responding to an anti-Hepatitis C viral therapy. These methods use the sequence and/or structure of the Hepatitis C RNA-dependent RNA polymerase NS5B to identify the emergence of a treatment-resistant Hepatitis C viral infection, particularly a benzofuran (e.g., HCV-796) treatment-resistant Hepatitis C viral infection. | 2010-02-04 |
| 20100028923 | Cross-reactive sensors - The present invention provides a novel cross-reactive sensor system utilizing cross-reactive recognition elements. In the inventive system, each of said one or more cross-reactive recognition elements is capable of interacting with more than one species of liquid analyte of interest, whereby each of said one or more cross-reactive recognition elements reacts in a different manner with each of said one or more species of liquid analytes of interest to produce a detectable agent of each analyte of interest, whereby said detectable agent is analyzed and the information is processed for data acquisition and interpretation. In certain preferred embodiments, the detectable agent and/or change is detected directly, while in certain other preferred embodiments, the detectable agent and/or change is detected with the help of a transducing agent capable of relaying information about each detectable agent generated for each of said species of liquid analyte of interest, whereby said information is processed for data acquisition and interpretation. The present invention also provides method for the analysis of analytes comprising contacting one or more analytes with the inventive system described above. | 2010-02-04 |
| 20100028924 | Method Of Diagnosing Pon1-Hdl Associated Lipid Disorders - Methods and kits for diagnosing a lipid-related disorder are disclosed. Methods and pharmaceutical compositions for treating lipid-related disorders are also disclosed. | 2010-02-04 |
| 20100028925 | METHOD AND DEVICE FOR THE ANALYSIS OF ENZYME ACTIVITY IN FLUIDS - An assay in which alkaline phosphatase is detected on one of a number of membranes held on a holder. A liquid sample and a substrate are combined on the membrane. If the enzyme is present, the substrate is cleaved. The products of this cleavage are optically detectable. | 2010-02-04 |
| 20100028926 | Novel enzymatic substrates derived from phenoxazinone and their use as developer in detection of microorganisms with peptidase activity - Novel enzymatic substrates of the general formula below: | 2010-02-04 |
| 20100028927 | SEAFOOD SPOILAGE INDICATOR - An enzyme based nondestructive sensor for the qualitative detection of spoilage in seafood is provided wherein the sensor does not alter the physical composition of the seafood specimen. The sensor comprises a sampling matrix, at least three or more enzymes in contact with the sampling matrix, and at least one indicator compound in contact with the sampling matrix. The enzymes are capable of interacting with four target chemicals comprising putrescine, cadaverine, histamine and tyramine, which are located on the surface of the seafood specimen. The indicator compound is capable of changing the color of the sampling matrix thereby indicating a qualitative visually detectable color change. A method for the nondestructive detection of the quality of a seafood specimen at any given time and for determining the remaining usable shelf life of the seafood specimen is disclosed. | 2010-02-04 |
| 20100028928 | Device for High-Throughput Stimulation, Immunostaining, and Visualizaion of Single Cells - Cell stimulation, staining, and visualization are common techniques in both clinical and research settings. The invention is directed to microfluidic devices for in situ cell stimulation, staining, and/or visualization, and related methods for applying one or more stimuli to the cells, and fixing and staining of cells in situ. The device allows for high-throughput screening of living cells using a minimal quantity of reagents where the fate of individual cells can be followed over time. | 2010-02-04 |
| 20100028929 | NUCLEIC ACID MOLECULES ENCODING GPR84 - The present invention is an isolated nucleic acid molecule encoding CD36, G | 2010-02-04 |
| 20100028930 | HEMATOPOIETIC CELL PHENOTYPING USING CIRCULATING CELL-FREE MARKERS - The present invention provides methods of classifying cluster of differentiation (CD) marker phenotype for hematopoietic cancer cells using multiple circulating cell-free CD markers in bodily fluid. In other aspects, treatment and disease progression of particular hematopoietic cancers can be monitored by measuring the levels of CD and other markers i bodily fluids of a patient. | 2010-02-04 |
| 20100028931 | Neurodegenerative diseases and methods of modeling - Disclosed are embryonic stem cells and motor neurons derived from mice carrying transgenic alleles of the normal or mutant human SOD1 gene. Also disclosed are in vitro systems employing such SOD1 transgenic motor neurons for the study of neural degenerative disease. | 2010-02-04 |
| 20100028932 | GENERAL PROGNOSTIC PARAMETERS FOR TUMOUR PATIENTS - Described is a method for identifying the prognosis for improved clinical benefit of an individual suffering from a tumor comprising providing a blood sample of said individual, (a1) determining the number of lymphocytes in the blood of said individual, and/or (a2) determining the number of neutrophils in the blood of said individual, and (b1) identifying the individual as having a good prognosis for improved clinical benefit, if the number of lymphocytes is above a lymphocyte baseline level of 1.4 to 1.8×10 | 2010-02-04 |
| 20100028933 | NUTRIENT MEDIUM UNIT AND METHOD FOR HOLDING A FILTER FROM A FILTRATION DEVICE - The invention relates to a nutrient medium unit for holding a filter of a filtration device, comprising a lower part that is filled with the nutrient medium and a lid, the latter having a fixing edge that protrudes into the lower part and that can be connected to an edge of the filter by means of an adhesive bond in order to remove said filter from the filtration device. The invention also relates to a method for the microbiological analysis of liquid samples, according to which a membrane filter is lifted off a filter support and laid on the surface of a nutrient medium that is situated in a lower part of a nutrient medium unit. The lower part is then covered by a lid, the latter being placed on the membrane filter lying on the filter support in such a way that a fixing edge located in the lid is connected to an edge of the filter by means of an adhesive bond. The lid and the attached filter are lifted off the filter support and placed on the dish-shaped lower part of the nutrient medium unit. | 2010-02-04 |
| 20100028934 | PIPETTE TIP AND A METHOD FOR PIPETTING A CONGEALED BLOOD SAMPLE UTILIZING THE PIPETTE TIP - A pipette tip and a method for pipetting a congealed blood sample utilizing the pipette tip are disclosed. The pipette tip includes an attachment section configured to be attached to a dispensing device, a liquid containing section configured to receive at least part of an aspired liquid sample, and a liquid aspiration section that is connected to the liquid containing section. The liquid aspiration section has a distal end aspiration opening and at least one further aspiration opening in a side wall. | 2010-02-04 |
| 20100028935 | CONVEX BOTTOM MICROWELL - Convex-bottom microwells for laboratory use in optical analyses of colorimetric or enzymological type useful for avoiding interferences with the luminous beam of the detecting instrument by corpuscular elements present in the sample to be analysed. | 2010-02-04 |
| 20100028936 | DETECTION OF CANCER CELLS IN VITRO USING SIGMA-2 RECEPTOR LIGANDS AS RADIOTRACERS - Methods for detection of cancer cells and for determining the proliferative status of cells in a tissue sample in vitro are disclosed. These methods comprise contacting a tissue sample with a radiolabeled compound or salt thereof of Formula | 2010-02-04 |
| 20100028937 | TEST STRIP FOR DETECTING GASTRIC PROBLEMS AND DETECTING METHOD THEREOF - The present invention provides a test strip and a detecting method thereof. The test strip comprises a first sheet, a second sheet a first filter and a second filter. The first sheet comprises a bulge portion deposited substantially in the center thereof. The second sheet comprises a depression in alignment with the bulge portion to form a sealing space. The first filter is disk-shaped and deposited in the sealing space. The first filter is used to receive a biological sample. The second filter is ring shaped and disposed in the sealing space and under the first filter to receive the biological sample coming from the first filter. The first filter comprises sodium azide, sodium dihydrogen phosphate, and urea, and the second filter comprises sodium azide, phenol red, and urea. | 2010-02-04 |
| 20100028938 | METHOD OF SCREEING FOR PATHOGENS - Disclosed is a method of screening for the presence of MRSA in a subject, the method comprising the steps of:
| 2010-02-04 |
| 20100028939 | Use of Galactose Oxidase for Selective Chemical Conjugation of Protractor Molecules to Proteins of Therapeutic Interest - This invention relates to novel compounds, methods for selective chemical conjugation of protractor molecules and the use thereof for diagnostic and/or therapeutic purposes. | 2010-02-04 |
| 20100028940 | Compositions and Methods for Metabolic Selection of Transfected Cells - The present invention relates to novel selection marker vectors, and methods for using these vectors to generate stable gene expression systems in eukaryotic cells utilizing any enzyme useful in the eukaryotic sterol/cholesterol biosynthetic pathway, such as a 3-ketosteroid reductase, as a metabolic selection marker to select transfected cells. In one embodiment, the method comprises transfecting cells that are auxotrophic for cholesterol with a vector encoding 3-ketosteroid reductase and at least one heterologous protein, and selecting cells that have the ability to survive in medium lacking cholesterol and/or producing the heterologous protein in these cells in chemically defined and/or serum-free media. | 2010-02-04 |
| 20100028941 | Method for Multiparallel Construction of Host/Vector-Systems for Expression of Proteins - The present invention relates to a multiparallel method for construction of host/vector-systems, comprising transforming a plurality of host strains, wherein substantially all host strains have deleterious mutations in at least one uptake system for a critical substrate needed for the growth of said host strain, with a plurality of vectors encoding at least one protein, and expressing said recombinant protein in said host strains. It further relates to host/vector systems obtainable by the method, to kits comprising the host strains and optionally the vectors, and to use of produced host/vector systems for protein expression. The method is exemplified using an | 2010-02-04 |
| 20100028942 | Methods for Improving Viability and Productivity in Cell Culture - Methods for increasing viability and production of secreted proteins in fed batch eukaryotic cell culture are disclosed. | 2010-02-04 |
| 20100028943 | Modified Messenger RNA Stabilizing Sequences for Expressing Genes in Bacterial Cells - The present invention relates to methods of producing a polypeptide having biological activity in a bacterial cell, comprising: (a) cultivating a bacterial host cell in a medium conducive for production of the polypeptide, wherein the bacterial host cell comprises a nucleic acid construct comprising a promoter region operably linked to a polynucleotide sequence encoding the polypeptide and a modified mRNA processing/stabilizing sequence located downstream of the promoter region and upstream of the ribosome binding site of the polynucleotide sequence encoding the polypeptide, wherein the modified mRNA processing/stabilizing sequence promotes higher expression of the polynucleotide sequence compared to an unmodified mRNA processing/stabilizing sequence; and (b) isolating the polypeptide having biological activity from the cultivation medium. The present invention also relates to such modified mRNA processing/stabilizing sequences, nucleic acid constructs, and bacterial host cells and to methods of obtaining such bacterial host cells. | 2010-02-04 |
| 20100028944 | Methods of obtaining genetic competence in bacillus cells - The present invention relates to methods of obtaining genetic competence in non-competent | 2010-02-04 |
| 20100028945 | LACTOBACILLUS ACIDOPHILUS NUCLEIC ACID SEQUENCES ENCODING STRESS-RELATED PROTEINS AND USES THEREFOR - Stress-related nucleic acid molecules and polypeptides and fragments and variants thereof are disclosed in the current invention. In addition, stress-related fusion proteins, antigenic peptides, and anti-stress-related antibodies are encompassed. The invention also provides recombinant expression vectors containing a nucleic acid molecule of the invention and host cells into which the expression vectors have been introduced. Methods for producing the polypeptides and methods of use for the polypeptides of the invention are further disclosed. | 2010-02-04 |
| 20100028946 | FUSION PARTNER CELLS - Fusion partner cells that enable production of heterohybridomas even from cells of species other than mouse were produced by fusing myeloma cells derived from a first animal species with leukemia cells derived from a second animal species, which have an extra S phase in the cell cycle and have the property of diploidizing. Stable production of substances can be achieved by producing heterohybridomas through cell fusion between the fusion partner cells and substance-producing cells of an animal other than mouse. | 2010-02-04 |
| 20100028947 | USE OF HUMAN CELLS OF MYELOID LEUKAEMIA ORIGIN FOR EXPRESSION OF ANTIBODIES - The invention relates to a method for producing a protein molecule composition having a defined glycosylation pattern, comprising (a) introducing in a host cell which is an immortalized human blood cell at least one nucleic acid encoding at least a part of said protein; and (b) culturing said host cell under conditions which permit the production of said protein molecule composition; and (c) isolating said protein molecule composition. | 2010-02-04 |
| 20100028948 | METHODS OF ADMINISTERING/DOSING ANTI-RSV ANTIBODIES FOR PROPHYLAXIS AND TREATMENT - The present invention encompasses novel antibodies and fragments thereof which immunospecifically bind to one or more RSV antigens and compositions comprising said antibodies and antibody fragments. The present invention encompasses methods preventing respiratory syncytial virus (RSV) infection in a human, comprising administering to said human a prophylactically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention also encompasses methods for treating or ameliorating symptoms associated with a RSV infection in a human, comprising administering to said human a therapeutically effective amount of one or more antibodies or fragments thereof that immunospecifically bind to one or more RSV antigens, wherein a certain serum titer of said antibodies or antibody fragments is achieved in said human subject. The present invention further encompasses compositions comprising antibodies or fragments thereof that immunospecifically bind to a RSV antigen, and methods using said compositions for detection or diagnosis a RSV infection. | 2010-02-04 |
| 20100028949 | SHRNA-MEDIATED INHIBITION OF EXPRESSION OF ALPHA 1,6-FUCOSYLTRANSFERASE - The current invention comprises a method for producing a heterologous polypeptide with a reduced degree of fucose modification in a mammalian cell by cultivating the mammalian cell under conditions suitable for the expression of said heterologous polypeptide, and recovering the heterologous polypeptide from the mammalian cell or the culture, wherein in said mammalian cell the enzymatic activity of α1,6-fucosyltransferase is reduced by means of an shRNA directed against α1,6-fucosyltransferase mRNA. | 2010-02-04 |
| 20100028950 | METHOD FOR THE IMMOBILIZATION OF BIOLOGICALLY ACTIVE POLYPEPTIDES BY USING MALTOSE BINDING PROTEIN - The present invention relates to a method for immobilization of a biologically active polypeptide using maltose binding protein (MBP) and a biologically active solid substrate on which a biologically active polypeptide is immobilized by the above method. More particularly, the present invention relates to a method for immobilization of a biologically active polypeptide comprising the following steps; 1) preparing a fusion protein by linking a biologically active polypeptide to carboxyl terminal of maltose binding protein (MBP); and 2) immobilizing the fusion protein on the hydrophobic surface by physical adsorption of amino terminal containing hydrophobic domain exposed on the surface of maltose binding protein on the hydrophobic surface of a solid substrate, and a biologically active solid substrate on which a biologically active polypeptide is immobilized by the said method. | 2010-02-04 |
| 20100028951 | PRODUCTION OF GLYCOPROTEINS WITH MODIFIED FUCOSYLATION - Methods are disclosed for genetically engineering host cells that lack an endogenous pathway for fucosylating N-glycans of glycoproteins to be able to produce glycoproteins with fucosylated N-glycans. | 2010-02-04 |
| 20100028952 | FERMENTATION AND PURIFICATION OF ACTINOMADURA CHROMOPROTEIN AND RELATED SPECIES - The present invention provides methods for production and purification of active chromoproteins produced by | 2010-02-04 |
| 20100028953 | Methods for production of oligonucleotides - The present invention relates to production of oligonucleotides using rolling circle replication, wherein synthesised multimeric oligonucleotides are reduced to mononucleotides using a nicking cassette. Thus, the invention provides a method for the production of oligonucleotides, enabling efficient amplification of oligonucleotides at lengths up to at least 1000 nucleotides and in high amounts contained within a nicking cassette. | 2010-02-04 |
| 20100028954 | Template-dependent nucleic acid polymerization using oligonucleotide triphosphate building blocks - A novel use of a template-dependent polymerase. The novel use is effected by employing the template-dependent polymerase for incorporating at least one oligonucleotide triphosphate onto a nascent oligonucleotide-3′-OH in a template-dependent manner. | 2010-02-04 |
| 20100028955 | Sequence Amplification with Target Primers - The present disclosure relates to the amplification of target nucleic acid sequences for various sequencing and/or identification techniques. This can be accomplished via the use of target primers and isothermal multiple strand displacement (MDA) processes. The use of these target primers and MDA, as described herein, allows for, for example, the reduction in the amplification of undesired hybridization events (such as primer dimerization and the “jackpot mutation” effect of PCR) while allowing for the amplification of the target nucleic acid sequences. | 2010-02-04 |
| 20100028956 | GENETICALLY ENGINEERED RECOMBINANT ESCHERICHIA COLI PRODUCING L-TRYPTOPHAN HAVING ORIGINALLY L-PHENYLALANINE PRODUCTIVITY, AND METHOD FOR PRODUCING L-TRYPTOPHAN USING THE MICROORGANISM - The present invention relates to a microorganism having L-tryptophan productivity and a method for producing L-tryptophan using the same. More precisely, the present invention relates to the recombinant | 2010-02-04 |
| 20100028957 | Microorganism Of Corynebacterium Genus Having Enhanced L-Lysine Productivity And A Method Of Producing L-Lysine Using The Same - The present invention relates to a microorganism of | 2010-02-04 |
| 20100028958 | METHOD FOR PRODUCTION OF L-AMINO ACID - A method for producing an L-amino acid is described, which is characterized by culturing a | 2010-02-04 |
| 20100028959 | METHOD FOR PRODUCTION OF L-AMINO ACID - The present invention has its object to provide a method for producing an L-amino acid comprising reacting a keto acid with an amino acid dehydrogenase and an enzyme having coenzyme regenerating ability to convert to a L-amino acid, wherein a coenzyme is added in two or more portions in the reaction. The method of the present invention enables efficient production of an L-amino acid useful as a synthetic intermediate such as a pharmaceutical intermediate with high optical purity by an enzymatic reductive amination independent of the purity of the keto acid used as a substrate. | 2010-02-04 |
| 20100028960 | Preparation of Precisely Controlled Thin Film Nanocomposite of Carbon Nanotubes and Biomaterials - Disclosed are nanocomposite materials comprising multiple layers of biomolecules bound to aligned carbon nanotubes. The thickness of each of the layers may be precisely controlled using a layer-by-layer assembly technique. | 2010-02-04 |
| 20100028961 | Biogical production of fuels - A process for the production of one or more fatty acids or derivatives thereof from a carbohydrate, which process comprises treating of Jerusalem Artichoke the carbohydrate with a micro-organism which converts the carbohydrate to a microbial oil comprising one or more fatty acids or derivatives thereof, in which the carbohydrate is inulin and/or is derived from Jerusalem Arti-choke. | 2010-02-04 |
| 20100028962 | Algal Medium Chain Length Fatty Acids and Hydrocarbons - The present invention provides methods and compositions for production of algal-based medium chain fatty acids and hydrocarbons. | 2010-02-04 |
| 20100028963 | SYSTEM FOR PRODUCING AROMATIC MOLECULES BY BIOCONVERSION - Yeast comprising at least one gene encoding vanillyl alcohol oxidase, the sequence of which is the sequence SEQ ID No. 1 or any sequence at least 70%, preferably 80%, very preferably 90% homologous to the sequence SEQ ID No. 1, and methods for producing coniferyl alcohol, ferulic acid and vanillin. | 2010-02-04 |
| 20100028964 | METHOD FOR PRODUCTION OF MICROBIAL FERMENTATION PRODUCT - The present invention relates to a method for production of a diol form represented by the formula (2), which includes filtering a culture fluid obtained by microbial conversion using a compound represented by the following formula (1a) and/or formula (1b) as a substrate, using a filter having an aperture size of 10 to 100 μm; washing the residue on the filter with water or a solvent having an SP value outside the range of 8.3 to 20 (cal/cm | 2010-02-04 |
| 20100028965 | Method for producing 1,3-propanediol using crude glycerol, a by-product from biodiesel production - The invention discloses a method for producing 1,3-propanediol, comprising the steps of: using crude glycerol, a by-product during the biodiesel production, without further treatment, as the substrate for production of 1,3-propanediol; inoculating a 1,3-propanediol-producing strain in a seed medium containing crude glycerol, a by-product from biodiesel production; adding the seed culture into a fermentation medium containing crude glycerol, a by-product from biodiesel production, and fermenting; maintaining pH in a range of 6.8 to 8.0; and in the end of the fermentation, isolating and purifying 1,3-propanediol. | 2010-02-04 |
| 20100028966 | Methods and Compositions for Improving The production Of Products In Microorganisms - Methods and compositions are provided for improving the production of products, such as fuel products like ethanol, in microorganisms. In particular, methods and compositions are described for improving ethanol production utilizing genes identified in | 2010-02-04 |
| 20100028967 | Method of changing property or function of substance and method of eliminating biofunction of cell - There are provided a method of changing a property or function of a substance by fragmenting a protein contained in the substance floating in a gas, and a method of eliminating a biofunction of a cell by applying to the cell either an electric discharge or a particle produced by the electric discharge, or both simultaneously, to fragment a protein contained in the cell. | 2010-02-04 |
| 20100028968 | BIOMECHANICAL TREATMENT FOR OBESITY AND DIABETES - Methods of maintaining or improving the metabolic state of a subject, e.g., a human, are disclosed. The methods can include providing to the subject a low magnitude, high frequency mechanical signal on a periodic basis and for a time sufficient to maintain or improve the subject's metabolic state. The subject can be diagnosed as having or can be at risk of developing, an obesity-related medical condition, e.g., type 2 diabetes, cardiovascular disease, hypertension, rheumatoid arthritis, and breast cancer. The methods can include a step of identifying a suitable subject by evaluating a physiological parameter that reflects the metabolic state of the subject, e.g., visceral fat content, subcutaneous fat content, body mass index, and blood pressure. | 2010-02-04 |
| 20100028969 | CELL LYSIS OR ELECTROPORATION DEVICE COMPRISING AT LEAST ONE PYROELECTRIC MATERIAL - The present invention relates to a cell lysis or electroporation device comprising at least one pyroelectric material. | 2010-02-04 |
| 20100028970 | Medium for the Production of Tiacumicin B - Methods, processes and materials for the production and recovery of Tiacumicins produced by culturing a microorganism belonging to the species | 2010-02-04 |
| 20100028971 | Carriers for Enzyme or Cell Immobilization and Immobilization Method Using the Carriers - The invention discloses a carrier made from an organic foam having open pores for enzymes or cells immobilization and the methods for preparing immobilized enzymes or cells. The invention uses flocculation and crosslinking technology to immobilize enzyme protein or cells on the organic foam material having open pores. The resultant immobilized products have larger specific surface area, higher specific activity and can be made into various shapes. | 2010-02-04 |
| 20100028972 | KETOREDUCTASE POLYPEPTIDES AND RELATED POLYNUCLEOTIDES - The present invention is directed to variant polypeptides having enhanced ketoreductase activity and/or thermostability for use in the stereospecific reduction of ketones. In addition, the present invention is directed to polynucleotides that encode the ketoreductase polypeptides, including codon optimized versions of the polynucleotides which provide for enhanced expression in host cells. In another aspect, the present invention is directed to nucleotide constructs, vectors and host cells that are transformed with polynucleotides of the present invention. | 2010-02-04 |
| 20100028973 | METHOD FOR PROLIFERATING HEPATITIS VIRUS, HOLLOW FIBER FOR CULTURING HEPATITIS VIRUS-INFECTED CELLS, AND USE THEREOF - The present invention provides (i) a method for proliferating a hepatitis virus, the method including the steps of: infecting, with the hepatitis virus, cells packed into a lumen of a hollow fiber made of a permeabilized membrane; and culturing the cells or (ii) a method for proliferating a hepatitis virus, the method including the steps of: infecting cells with the hepatitis virus; packing the cells into a lumen of a hollow fiber made of a permeabilized membrane; and culturing the cells. This provides an experimental system for infecting in vitro culture cells with a hepatitis virus such as HBV or HCV and then proliferating the hepatitis virus. | 2010-02-04 |
| 20100028974 | NOVEL BACILLUS BACTERIUM STRAIN CAPABLE OF DECOMPOSING/VOLUME-REDUCING PLANT RESIDUE - Disclosed is a method for decomposing a plant residue and reducing a volume of the plant residue to a satisfactory level within a short period. Also disclosed is a bacterium for use in the method. Further disclosed is a material for decomposing a plant residue and reducing a volume of the plant residue, which contains the bacterium. A bacterium is found which is capable of decomposing a plant residue and reducing a volume of the plant residue under natural conditions satisfactorily and stably. The bacterium can be used for decomposing a plant residue and reducing a volume of the plant residue. A material containing cells of | 2010-02-04 |
| 20100028975 | TRAITS IN RECOMBINANT XYLOSE-GROWING SACCHAROMYCES CEREVISIAE STRAINS USING GENOME-WIDE TRANSCRIPTION ANALYSIS - New xylose-utilizing | 2010-02-04 |
| 20100028976 | PHOTOBIOREACTOR AND USES THEREFOR - The present invention provides novel photobioreactors, modules thereof, and methods for use in culturing and harvesting algae and cyanobacteria. | 2010-02-04 |
| 20100028977 | ENCLOSED PHOTOBIOREACTORS WITH ADAPTIVE INTERNAL ILLUMINATION FOR THE CULTIVATION OF ALGAE - The present invention provides an enclosed, internally-lighted bioreactor apparatus, methods for growing photosynthetic microorganisms in the enclosed, internally lighted bioreactor and methods for enhancing growth of a photosynthetic microorganism culture in the enclosed internally-lighted bioreactor. | 2010-02-04 |
| 20100028978 | IN SITU HEAT INDUCED ANTIGEN RECOVERY AND STAINING APPARATUS AND METHOD - An automated microscope slide staining system and staining apparatus and method that features a plurality of individually operable miniaturized pressurizable reaction compartments or a pressurizable common chamber for individually and independently processing a plurality of microscope slides. The apparatus preferably features independently movable slide support elements each having an individually operable heating element. | 2010-02-04 |
| 20100028979 | METHODS AND APPARATUS FOR ORGAN SUPPORT - An organ support apparatus includes: (a) a fluid circuit defining upstream and downstream legs adapted to be connected to an organ to be supported; (b) a circulation pump connected to the fluid circuit for circulating a process fluid through the fluid circuit and the organ; and (c) a first waveform generator connected to the fluid circuit for impressing a preselected pressure waveform on the process fluid. | 2010-02-04 |
| 20100028980 | REAL-TIME PCR IN MICRO-CHANNELS - The present invention relates to methods for amplifying nucleic acids in micro-channels. More specifically, the present invention relates to methods for performing a real-time polymerase chain reaction (PCR) in a continuous-flow microfluidic system and to methods for monitoring real-time PCR in such systems. | 2010-02-04 |
| 20100028981 | SPECIMEN COLLECITON AND ASSAY CONTAINER - The present invention includes but is not limited to a specimen collection device that includes a chamber capable of collecting a specimen, a specimen passage slot, a reservoir, a reservoir seal, and a test device. A sample or specimen added to the chamber flows through the specimen passage slot into the reservoir. Flow into the reservoir may be limited by the reservoir seal. The test device positioned within the reservoir detects the presence or concentration of an analyte within the sample or specimen. | 2010-02-04 |
| 20100028982 | DEVICE FOR, AN ARRANGEMENT FOR AND A METHOD OF ANALYSING A SAMPLE | 2010-02-04 |
| 20100028983 | MICROWAVE ACCELERATED ASSAYS - The present invention provides for increasing fluorescence detection in surface assay systems while increasing kinetics of a bioreaction therein by providing low-power microwaves to irradiate metallic materials within the system in an amount sufficient to increase heat thereby affecting the kinetics of a bioreaction therein. | 2010-02-04 |
| 20100028984 | DEVICES AND METHODS FOR BIOCHIP MULTIPLEXING - The invention is directed to a method of analyzing a plurality of biochips. In particular, the method includes inserting a first biochip in to a first station of an analysis device, inserting a second biochip into a second station of the analysis device, wherein each of the first and second biochips comprise a substrate that includes an array of detection electrodes, each including a different capture binding ligand, a different target analyte, and a label, and a plurality of electrical contacts, detecting current as an indication of the presence of the labels on the first biochip and detecting current as an indication of the presence of the labels on the second biochip. | 2010-02-04 |
| 20100028985 | Reaction Vessel, Reaction Vessel Processing Apparatus and Diagnostic Apparatus - A reaction vessel suitable for automating of various measurements is provided. In a preferred embodiment, on the same side of plate-like substrate ( | 2010-02-04 |
| 20100028986 | REACTION CONTAINER PLATE AND REACTION TREATMENT APPARATUS - Disclosed herein is a reaction container plate which prevents the entry of foreign matter from the outside and the pollution of an outside environment. A reaction container plate ( | 2010-02-04 |
| 20100028987 | METHOD FOR IMPROVING HEAT STABILITY OF COMPOSITION CONTAINING WATER-SOLUBLE COENZYME-BOUND GLUCOSE DEHYDROGENASE (GDH) - The invention relates to a method for improving the heat stability of glucose dehydrogenase (GDH). The invention provides a method for preparing a glucose sensor with soluble flavin-bound GDH by providing a composition comprising soluble flavin-bound GDH at an acidic pH and heat drying the composition onto a glucose sensor. | 2010-02-04 |
| 20100028988 | Microplates with Ultra-Thin Walls by Two-Stage Forming - A multi-well plate with wells having ultra-thin walls and yet with sufficient structural rigidity to allow reliable use of the plate in automated instrumentation is formed by first forming a plate blank by injection molding, the blank being of a thickness that provides the desired rigidity, and then subjecting the blank to vacuum forming to stretch designated areas in the blank to form wells or to extend wells already formed, the stretching resulting in a reduction in thickness of the molded resin at the walls of the wells only. | 2010-02-04 |
| 20100028989 | MONITOR DEVICE AND BIOLOGICAL COMPONENT-MEASURING DEVICE - This invention provides a monitor device capable of detecting possible abnormalities in body fluid withdrawal by a body fluid sampler and variations in the dilution when a withdrawn body fluid is diluted, and a living organism-measuring device with this monitor device. The former device includes a first optical sensor disposed on a diluent channel through which a diluent is introduced into a body fluid sampler for withdrawing a body fluid; a second optical sensor disposed on a diluted body fluid channel through which the diluted body fluid is transferred to a living organism-measuring sensor, and close to the first optical sensor; and a controlling unit for judging whether variations in a dilution to which the body fluid is diluted are within predetermined limits, from data outputted by the first optical sensor and data outputted by the second optical sensor. The latter device has the monitor device incorporated thereto. | 2010-02-04 |
| 20100028990 | STERILE BIOREACTOR BAG WITH INTEGRATED DRIVE UNIT - Flexible, sterilizable, disposable bioreactors are provided with integrated fluidic drive units that agitate media inside the bioreactor without introducing contamination. The bioreactor system ( | 2010-02-04 |
| 20100028991 | Asymmetric compound parabolic concentrator and related systems - Various embodiments disclosed herein provide for an asymmetric compound parabolic concentrator (ACPC), a liquid light guide, and an algae mixer. These embodiments may be used singularly or in combination, for example, as part of a bioreactor. In various embodiments an ACPC may be used to capture light without the aid of motors or positioning. As such, the ACPC may be used despite seasonal solar variations. The illuminating light guide may be used to guide light, for example, from an exit aperture of an ACPC, through walls substantially perpendicular to the entrance window of the illuminating light guide. In some embodiments, the illuminating light guide may provide substantially uniform light distribution through a vertical profile. Other embodiments include, for example, an algae mixer and bioreactors. | 2010-02-04 |
| 20100028992 | Cell culture device - The present invention discloses a cell culture device, including a container, and a carrying assembly; the container has an opening and holds the cell culture medium, and the carrying assembly is immersed in the cell culture solution from the opening of the container. The present invention also discloses a carrying assembly used for cell culture, including a cover, a supporter, and a vessel, wherein the cover and the vessel are connected by the supporter. | 2010-02-04 |
| 20100028993 | METHODS OF PRODUCING PEPTIDES IN PLANTS AND PEPTIDES PRODUCED THEREBY - Methods of increasing the yield in plant expression of recombinant proteins comprising engineering glycosylation sites into cloned genes or cDNAs for proteins using codons that drive post-translational modifications in plants and engineering the cloned genes or cDNAs to contain a plant secretory signal sequence that targets the gene products (protein) for secretion are present. The methods result in increased recombinant glycosylated protein yields. Proteins produced according to these methods are also present. | 2010-02-04 |
