05th week of 2013 patent applcation highlights part 44 |
Patent application number | Title | Published |
20130029319 | MEANS AND METHODS FOR PREDICTING THE RISK OF MORTALITY OF PATIENTS WITH HPV POSITIVE OROPHARYNGEAL SQUAMOUS CELL CANCER - The present invention relates to the field of diagnostic measures. In particular, the present invention relates to a method for predicting the risk of mortality in a subject suffering from low viral load HPV (human papillomavirus) positive oropharyngeal squamous cell cancer. The method is based on the determination of the amount of an E6* gene product and the amount an E1̂E4 gene product of HPV in a sample from said subject. Moreover, the method is based on determining the presence of absence of an E1C gene product of HPV. The present invention further relates to a method for predicting the risk of mortality in a subject suffering from HPV (human papillomavirus) positive oropharyngeal squamous cell cancer based on the determination of copy number of HPV per cancer cell. | 2013-01-31 |
20130029320 | DEVICE AND METHOD FOR DETECTING BIOMOLECULE - Disclosed are a method for detecting a biomolecule including: immobilizing a nucleic acid aptamer capable of specifically binding to a biomolecule to be detected on the surface of a bead on which fluorophores are arranged; hybridizing the nucleic acid aptamer with a guard nucleic acid (g-nucleic acid) labeled with a quencher to quench fluorescence; and reacting a sample including the biomolecule to be detected with the nucleic acid aptamer and detecting a fluorescence signal emitted as the biomolecule binds with the nucleic acid aptamer and the g-nucleic acid labeled with the quencher is separated, and a device for detecting a biomolecule for conducting the detection method. The present disclosure allows for effective, convenient and fast detection of the biomolecule to be detected, enables quantitative analysis, and enables detection of even a trace amount of sample. | 2013-01-31 |
20130029321 | SAMPLE PROCESSING DEVICE AND METHOD - A sample processing device is disclosed, which sample processing device comprises a first substrate and a second substrate, where the first substrate has a first surface comprising two area types, a first area type with a first contact angle with water and a second area type with a second contact angle with water, the first contact angle being smaller than the second contact angle. The first substrate defines an inlet system and a preparation system in areas of the first type which two areas are separated by a barrier system in an area of the second type. The inlet system is adapted to receive a sample liquid comprising the sample and the first preparation system is adapted to receive a receiving liquid. In a particular embodiment, a magnetic sample transport component, such as a permanent magnet or an electromagnet, is arranged to move magnetic beads in between the first and second substrates. | 2013-01-31 |
20130029322 | ANTI-HPV E7 ANTIBODIES - Monoclonal anti-HPV (human papillomavirus) E7 antibodies are capable of specifically recognizing an epitope of the C-terminal or the N-terminal region of a HPV E7 protein. | 2013-01-31 |
20130029323 | SYSTEMS, DEVICES, AND METHODS FOR AUTOMATED CHARACTERIZATION OF A NUCLEIC ACID MOLECULE - The invention generally relates to systems, cartridges, and methods for automated characterization of a nucleic acid molecule, in particular, optical mapping of DNA from an organism. In certain embodiments, the invention provides a cartridge for characterizing a nucleic acid molecule, the cartridge including a reaction chamber having a derivatized bottom surface, at least one reagent reservoir, and a pump, in which the reaction chamber, the reagent reservoir, and the pump are fluidically connected to each other. | 2013-01-31 |
20130029324 | LIVE BIOLOAD DETECTION USING MICROPARTICLES - The present invention provides methods to concentrate cells onto microparticles, to concentrate the microparticles, and to detect the cells. The present invention also includes unitary sample preparation and detection devices to be used in accordance with the methods. | 2013-01-31 |
20130029325 | FLUORESCENT PROBE FOR PLASMA CELL IDENTIFICATION AND ISOLATION, AND PLASMA CELL IDENTIFICATION OR ISOLATION METHOD USING THE PROBE - A method which can isolate plasma cells and plasmablasts efficiently and with high purity, from mammals and birds, without using a cell surface marker is provided. | 2013-01-31 |
20130029326 | Selective 5' Ligation Tagging of RNA - The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′ tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses. | 2013-01-31 |
20130029327 | GENETIC POLYMORPHISMS ASSOCIATED WITH LIVER FIBROSIS, METHODS OF DETECTION AND USES THEREOF - The present invention is based on the discovery of genetic polymorphisms that are associated with liver fibrosis and related pathologies. In particular, the present invention relates to nucleic acid molecules containing the polymorphisms, including groups of nucleic acid molecules that may be used as a signature marker set, variant proteins encoded by such nucleic acid molecules, reagents for detecting the polymorphic nucleic acid molecules and proteins, and methods of using the nucleic acid and proteins as well as methods of using reagents for their detection. | 2013-01-31 |
20130029328 | METHODS AND KITS FOR THE IDENTIFICATION OF ANIMALS HAVING A GREATER POTENTIAL FOR DESIRABLE CHARACTERISTICS, AND FOR THE EARLY IDENTIFICATION OF FAT DEPOSITS IN BOVINES - The present invention relates to a method and a kit for the identification of animals having greater potential for desirable characteristics of meat quality, rib eye area (REA), weaning weight and 18-month weight by means of the analysis of specific markers. | 2013-01-31 |
20130029329 | DETECTION OF AAD-12 SOYBEAN EVENT 416 - The invention relates in part to methods of detecting an AAD-12 soybean event. The subject invention provides assays for detecting the presence of the subject event in a sample (of soybeans, for example). Kits and conditions useful in conducting the assays are also provided. More specifically, the present invention relates in part to an endpoint TaqMan PCR assay for the AAD-12 soybean event. Some embodiments are directed to assays that are capable of high throughput zygosity analysis. The subject invention further relates, in part, to the discovery of a preferred reference gene for use in determining zygosity. This invention also relates in part to plant breeding using any of the subject methods. In some embodiments, said event/polynucleotide sequence can be “stacked” with other traits. The subject procedures can be used to uniquely identify soybean lines comprising the event of the subject invention. | 2013-01-31 |
20130029330 | MUTANT LDL RECEPTOR GENE - There is disclosed a method of identifying individuals susceptible to familial hypercholesterolemia and which method comprises identifying in a sample from said individual at least one polymorphism at position 1706-2 of the coding region (41902 of the genomic DNA) in the low density lipoprotein receptor gene, and wherein the presence of at least one said polymorphism is indicative of said individual being of a higher susceptibility to familial hypercholesterolemia. | 2013-01-31 |
20130029331 | NOVEL FLUORESCENCE-BASED ASSAY FOR THE RAPID DETECTION AND QUANTIFICATION OF DEOXYRIBONUCLEOSIDE TRIPHOSPHATES - The inventors have developed a rapid and sensitive fluorescence-based assay to quantify dNTPs. This assay relies on the principle that incorporation of a limiting dNTP is required for primer-extension and polymerase-mediated 5-3′ exonuclease hydrolysis of a quenched fluorophore-labeled probe resulting in fluorescence. The concentration of limiting dNTPs is directly proportional to the fluorescence generated. This assay has important applications in research that investigates the influence of pathological conditions or pharmacological agents on dNTP biosynthesis and regulation. | 2013-01-31 |
20130029332 | COMPOSITIONS AND METHODS FOR DETECTING NOONAN SYNDROME - Diagnostic and therapeutic applications for Noonan Syndrome are described. The diagnostic and therapeutic applications are based on certain mutations in a RAS-specific guanine nucleotide exchange factor gene SOS1 or its expression product. The diagnostic and therapeutic applications are also based on certain mutations in a serine/threonine protein kinase gen RAF1 or its expression product thereof. Also described are nucleotide sequences, amino acid sequences, probes, and primers related to RAF1 or SOS1, and variants thereof, as well as host cells expressing such variants. | 2013-01-31 |
20130029333 | Magnetic Bead Quantum Dot Nanoparticle Assay - The present disclosure includes a magnetic bead (MB) quantum dot (QD) nanoparticle assay for detecting, capturing, separating, and/or quantifying a target in a sample. | 2013-01-31 |
20130029334 | mRNA As Biomarkers For Liver Injury or Other Liver Perturbations - The present invention provides a method for assessing the likelihood or for detecting the presence of liver perturbation in an individual. The method is particularly useful for detecting drug-induced liver injury (DILI) or other forms of hepatotoxicity or hepatic perturbation. The method comprises measuring the level of at least one RNA biomarker comprising mRNA and comparing the measured level to an appropriate reference value for the sample type and RNA biomarker type, and wherein a significant difference between the measured level and the reference value is indicative of liver perturbation. | 2013-01-31 |
20130029335 | SPINOCEREBELLAR ATAXIA TYPE 8 AND METHODS OF DETECTION - The present invention provides an isolated nucleic acid molecule containing a repeat region of an isolated spinocerebellar ataxia type 8 (SCA8) coding sequence, the coding sequence located within the long arm of chromosome 13, and the complement of the nucleic acid molecule. Diagnostic methods based on identification of this repeat region are also provided. | 2013-01-31 |
20130029336 | KRAS Primers and Probes - The present invention provides oligonucleotide primers or probes for the detection of a mutation of the KRAS gene. The invention also provides a method for detecting a mutation in the KRAS gene using the oligonucleotide primers or probes disclosed therein. Furthermore, the present invention encompasses a method for predicting the sensitivity of a tumor in a patient to epidermal growth factor receptor-directed chemotherapy, comprising obtaining DNA from the tumor; and determining whether there is a mutation in codon 12 and/or a mutation in codon 13 in exon 2 of the KRAS gene in the DNA using a method utilizing at least one of the oligonucleotide primers and/or probes of the present invention. | 2013-01-31 |
20130029337 | METHODS AND USES INVOLVING GENETIC ABNORMALITIES AT CHROMOSOME 12 - The present invention relates to the fields of genetics and oncology and provides methods for predicting and identifying tumors of epithelial origin. Specifically, the present invention relates to a novel method of predicting tumor initiation, tumor progression and/or carcinomas, the method comprising detecting genetic abnormality associated with tumors of epithelial origin. The present invention further relates to a novel method of identifying an individual with potential for developing carcinoma, the method comprising detection of genetic abnormalities. The present invention also relates to a method of predicting the progression of carcinomas and the transformation thereof to an aggressive variant, the method comprising detection of genetic abnormalities, which indicate the probability to develop carcinoma. The present invention also relates to a use of specific chromosomal region, a gene or a fragment thereof, and/or genetic markers for predicting tumor initiation, tumor progression and/or carcinoma. The present invention also relates to a use of specific chromosomal region or a gene or a fragment thereof in therapy, for the development of therapy, and for the preparation of a medicament for treating tumors of epithelial origin. | 2013-01-31 |
20130029338 | MICROFLUIDIC DEVICES - Methods and devices for the interfacing of microchips to various types of modules are disclosed. The technology disclosed can be used as sample preparation and analysis systems for various applications, such as DNA sequencing and genotyping, proteomics, pathogen detection, diagnostics and biodefense. | 2013-01-31 |
20130029339 | USE OF MICROVESICLES IN ANALYZING KRAS MUTATIONS - Microvesicles are small membrane vesicles that either shed or bud off eukarotic cells. Analysis of the nucleic acid content of microvesicles may be useful in detecting the presence or absence of genetic aberrations. This invention discloses novel methods of diagnosing, prognosing, monitoring, or treating a disease, such as cancer, or other medical condition in a subject involving analyzing one or more nucleic acids contained within an isolated microvesicle for the presence or absence of one or more Kras genetic aberrations. | 2013-01-31 |
20130029340 | METHOD FOR DETECTING MORE THAN ONE TARGET IN A PCR-BASED APPROACH APPLYING AN UN-SPECIFIC DYE WHICH IS NOT INTERFERING WITH THE EMISSION OF FLUOROPHORE-LABELED PROBES - The present invention relates to a method for detecting more than one target with sequence-specific probes and to distinguish these targets at the same time during melting point analysis. | 2013-01-31 |
20130029341 | METHOD TO AMPLIFY NUCLEIC ACIDS TO GENERATE FLUORESCENCE LABELED FRAGMENTS OF CONSERVED AND ARBITRARY PRODUCTS - Disclosed herein are methods for the identification of the species, serotype, and strain of a microorganism. Also disclosed are primers for use in detecting such microorganisms and kits comprising such primers. | 2013-01-31 |
20130029342 | GLYCINE RIBOSWITCHES, METHODS FOR THEIR USE, AND COMPOSITIONS FOR USE WITH GLYCINE RIBOSWITCHES - Riboswitches are structural elements in mRNA that change state when bound by a trigger molecule, and are thus able to regulate gene expression. They can be dissected into two separate domains: one that selectively binds the target (aptamer domain) and another that influences genetic control (expression platform domain). Bacterial glycine riboswitches consist of two tandem aptamer domains which cooperatively bind glycine to regulate the expression of downstream genes. These natural switches are targets for antibiotics and other small molecule therapies. Modified versions of these natural riboswitches can be employed as designer genetic switches that are controlled by specific effector compounds. Disclosed are isolated and recombinant riboswitches, and compositions and methods for selecting and identifying compounds that can activate, inactivate, or block a riboswitch. | 2013-01-31 |
20130029343 | MULTIWELL STRIPS - The present invention relates to a multiwell strip comprising a frame portion consisting of a first stiff material, and a plurality of wells arranged in a line and consisting of a second material, which is not as stiff as the first material. Furthermore, the present invention relates to a multiwell-frame for obtaining a multiwell strip according to the present invention and methods for producing a multiwell strip according to the present invention. | 2013-01-31 |
20130029344 | TAGGED OLIGONUCLEOTIDES AND THEIR USE IN NUCLEIC ACID AMPLIFICATION METHODS - The present invention provides nucleic acid amplification systems and methods that desirably reduce or eliminate false positive amplification signals resulting from contaminating biological material, e.g., nucleic acid, that may be present in one or more reagents used in an amplification reaction and/or that may be present in the environment in which an amplification reaction is performed. The invention offers the further advantage of requiring less stringent purification and/or sterility efforts than conventionally needed in order to ensure that enzymes and other reagents used in amplification reactions, and the environment in which an amplification reaction is performed, are free of bacterial or other nucleic acid contamination that may yield false positive results. | 2013-01-31 |
20130029345 | NMR SYSTEMS AND METHODS FOR THE RAPID DETECTION OF ANALYTES - This invention features systems and methods for the detection of analytes, and their use in the treatment and diagnosis of disease. | 2013-01-31 |
20130029346 | VECTOR AND SCREENING ASSAY FOR CD44 EXPRESSING CARCINOMAS - The present invention relates, in part, to the discovery of cis-regulatory regions for the expression of CD44 in normal cells and/or and over-expression in cancer cells or cancer stem cells. To this end, the present invention provides isolated DNA, vectors, kits, and methods that may be used for the evaluation and/or screening one or more therapeutic agents for the treatment of a CD44 expressing carcinoma. | 2013-01-31 |
20130029347 | DEVICES AND METHODS FOR IMMOBILIZING NUCLEIC ACIDS - The present invention generally relates to devices and methods for immobilizing nucleic acids on a substrate. In certain embodiments, devices of the invention include a voltage source, and a substrate coupled to the voltage source, in which hydrophobicity of the substrate changes in response to an applied electric field and a surface of the substrate is coated with a substance that retains nucleic acids. | 2013-01-31 |
20130029348 | METHODS OF ELONGATING NUCLEIC ACIDS - The invention generally relates to methods for elongating nucleic acid, such as DNA, on a charged substrate. In certain embodiments, methods of the invention involve contacting an applicator to a sample including a nucleic acid, and swabbing the applicator on a charged substrate, thereby elongating the nucleic acid on the substrate. | 2013-01-31 |
20130029349 | ASSAYS FOR THE DETECTION OF MICROBES - Methods, devices, and kits are provided herein for the accurate and rapid detection of disease causing microbes in a sample by the detection of microbial components of which correlate to the presence of the microbe. Kits include a first binding agent operatively coupled to an immobilized support; and a second binding agent operatively coupled to one or more pH indicating moieties wherein the first and second binding agents bind with sufficient specificity to the microbial component to permit detection of that component which correlates to the presence of the microbe in the sample. | 2013-01-31 |
20130029350 | BI-STABLE OSCILLATOR - In accordance with the present invention there is provided a bi-stable oscillator circuit for detecting a load imparted to a surface. The bi-stable oscillator comprises an electrical amplifier, at least one resonator comprising an electrical transducer having a resonant frequency, a surface of the resonator forming the surface on which the load is to be detected and an impedance network having a resonant frequency. The resonator is arranged to be exposed to an environment under test, and the resonator and the impedance network are connected in parallel with the electrical amplifier such that when a load imparted to the surface exceeds a pre-determined threshold value the oscillator circuit oscillates at the resonant frequency of the impedance network and when a load imparted to the surface is less than the threshold value the oscillator circuit oscillates at the resonant frequency of the resonator, switching of the circuit oscillation frequency being indicative of the load crossing the predetermined threshold value. | 2013-01-31 |
20130029351 | A25 BACTERIOPHAGE LYSIN - The invention relates to the identification, sequencing, and isolation of an A25 bacteriophage lysin gene that expresses a protein involved in the lysis of bacterial cells during the phage life cycle. The invention further relates to methods for lysing certain bacteria using lysin, which are useful for example in a diagnostic procedure designed to detect these bacteria. | 2013-01-31 |
20130029352 | METHODS FOR DIAGNOSING AND MONITORING THE PROGRESSION OF CANCER - Methods for measuring c-Met levels in urine and blood samples are provided. Methods for diagnosis and prognosis evaluation for cancer are also provided. | 2013-01-31 |
20130029353 | AUTOANTIBODY ENHANCED IMMUNOASSAYS AND KITS - The present disclosure provides immunoassays and kits for detection or quantification of an analyte of interest in a test sample that potentially contains endogenously produced autoantibodies reactive with the analyte | 2013-01-31 |
20130029354 | MULTIPLE SEPARATION DEVICE AND METHOD FOR SEPARATING CANCER CELLS IN BLOOD USING THE DEVICE - Provided are a multiple separation device and a method of separating cancer cells in blood using the device. In this device and method, twice magnetophoresis separation steps are performed. At a second magnetophoresis separation step, shapes of ferromagnetic patterns are changed to separate cancer cells into cancer kinds. | 2013-01-31 |
20130029355 | MULTIPLE ANALYSIS DEVICE AND METHOD FOR ANALYZING CANCER CELLS IN BLOOD - Provided are a multiple analysis device and a method of analyzing cancer cells in blood using the device. In this device and method, it can analyze the cancer cells along cancer kinds by using the magnetic nanoparticles combined to the markers of the cancer cells and the difference of the magnetic fields of them. | 2013-01-31 |
20130029356 | METHODS AND SYSTEMS FOR USING DRUGS AS BIOMARKERS - Methods and systems for using drugs as biomarkers to investigate the status of biological systems are disclosed. A drug is conjugated with a light emitting dye that emits light in a channel of the electromagnetic spectrum when an appropriate stimulus is applied. In one aspect, a suspension suspected of containing target particles is added to a tube along with the conjugated drug/dye complex and a float. Centrifugation of the tube, float, and suspension causes various components to separate along the axial length of the tube. Binding of the drug/dye complex to the target particles can be assessed by applying an appropriate stimulus to the tube, which, in turn, causes the fluorescent dyes to emit light in the channel. The level of fluorescence of the target particles located between the float and the inner wall of the tube can be used to assess use of the drug in therapy. | 2013-01-31 |
20130029357 | DIAGNOSTIC MARKER FOR EFFECT OF ANTICANCER AGENT - The present invention enables to realize a convenient determination of a therapeutic effect of an anticancer agent on a cancer. Specifically, the present invention provides a diagnostic marker for an effect of an anticancer agent on a cancer, comprising a substance having an affinity for a fragment of an extracellular domain of c-MET; a diagnostic reagent for an effect of an anticancer agent on a cancer, comprising a substance having an affinity for a fragment of an extracellular domain of c-MET and the fragment of the extracellular domain of c-MET; and a method of testing an effect of an anticancer agent on a cancer, comprising (a) measuring a concentration of a fragment of an extracellular domain of c-MET in a biological sample from a subject, and (b) comparing the measured concentration of the fragment of the extracellular domain of c-MET with an indicator which presents a relationship between a concentration of the fragment of the extracellular domain of c-MET and the effect of the anticancer agent on the cancer. | 2013-01-31 |
20130029358 | IMMUNODOMINANT MHC DR52B RESTRICTED NY-ESO-1 EPITOPES, MHC CLASS II MONOMERS AND MULTIMERS, AND USES THEREOF - Immunostimulatory NY-ESO-1 epitopes recognized by MHC-DRB3*0202 (DR52b) or DRB1*0101 (DR1) restricted T cells are described. Methods for their use in diagnostic and therapeutic approaches are also provided. Further, methods for the generation and isolation of MHC class II molecules, either “empty” or peptide-loaded, are provided. Methods for the assembly of MHC class II multimers, for example, tetramers, are also provided. Methods for the detection of T cells binding to specific peptide-loaded MHC class II molecules are also described herein. | 2013-01-31 |
20130029359 | MONOCLONAL ANTIBODIES AND DIAGNOSTIC USES THEREOF - The disclosure relates to antibodies to the preferentially expressed antigen in melanoma (PRAME), and the synovial sarcoma X breakpoint 2 (SSX-2) antigens, methods of use, and diagnostic kits thereof. In exemplary embodiments, the disclosure relates to monoclonal antibodies to specific epitopes of the PRAME and SSX-2 antigens and methods of using such antibodies. | 2013-01-31 |
20130029360 | DIMERIC CORE-SHELL NANOSTRUCTURE LABELED WITH RAMAN ACTIVE MOLECULE LOCALIZED AT INTERPARTICLE JUNCTION, USE THEREOF, AND METHOD FOR PREPARING THE SAME - The present invention relates to a nanoparticle dimer in which Raman-active molecules are located at a binding portion of the nanoparticle dimer, and more particularly, to a core-shell nanoparticle dimer comprising: a gold or silver core having a surface to which oligonucleotides are bonded; and a gold or silver shell covering the core. In addition, the present invention relates to the core-shell nanoparticle dimer, to a method for preparing same, and to the use thereof. | 2013-01-31 |
20130029361 | DISC-SHAPED ANALYSIS CHIP - A disc-shaped analysis chip has an internal space. The internal space includes: a first reservoir for accommodating a first liquid; a second reservoir and a third reservoir arranged nearer to an outer peripheral portion of the analysis chip than the first reservoir; a fourth reservoir, a fifth reservoir and a sixth reservoir for accommodating a second liquid, a third liquid and a fourth liquid, respectively, and being arranged nearer to the outer peripheral portion of the analysis chip than the second and the third reservoir; a seventh reservoir arranged nearer to the outer peripheral portion of the analysis chip than the fourth to the sixth reservoir; an eighth reservoir arranged nearer to the outer peripheral portion of the analysis chip than the seventh reservoir; and a first to an eighth flow path for appropriately interconnecting the first to the eighth reservoir. | 2013-01-31 |
20130029362 | MARKERS AND ASSAYS FOR DETECTION OF NEUROTOXICITY - A process and assay for diagnosing neurotoxicity in a subject is provided. The extent of a neurotoxic insult to a subject is assessed through the measurement of one or more biomarkers in a biological fluid, such as CSF or serum. Other uses and advantages afforded include pre-market drug discovery, monitoring, drug neurotoxicity screening and post market assessment of safety and monitoring for drug of known potential neurotoxicity. | 2013-01-31 |
20130029363 | METHOD FOR DIAGNOSING MALIGNANT TUMOR - To provide a method and a diagnostic kit for determining the presence of a malignant tumor or the severity thereof, a method for selecting a therapeutic method therefor or evaluating the effect of the therapeutic method, or a method for estimating the risk of recurrence of the malignant tumor or determining the presence or absence of the recurrence. | 2013-01-31 |
20130029364 | METHOD FOR IMMOBILIZING GLUCOSE OXIDASE ON A SELF-ASSEMBLED MONOLAYER - Provided are a method for increasing an amount of glucose oxidase to be immobilized on the self-assembled monolayer and a sensor which comprises glucose oxidase immobilized with the method. The method of the disclosed technology is characterized by that one molecule of an amino acid is interposed between the self-assembled monolayer and the molecule of the glucose oxidase. | 2013-01-31 |
20130029365 | MULTI-DIMENSIONAL CHROMATOGRAPHIC METHODS FOR SEPARATING N-GLYCANS - A multi-dimensional chromatographic method for the separation of N-glycans. The method comprises providing a glycan preparation that includes at least one negatively charged N-glycan. The glycan preparation is then separated by anion-exchange chromatography and at least one secondary chromatographic technique. | 2013-01-31 |
20130029366 | PTP1B INHIBITORS - Described herein are agents, including antibodies, antibody fragments, polypeptides and organic small molecules, that bind reversibly inactive PTP1B (designated herein in the alternative as PTP1B-SN or PTP1B-OX) and stabilize it (stabilize PTP1B-OX) in such a manner that they inhibit reactivation of reversibly oxidized, inactive PTP1B by reduction (by reducing agent) and have no substantial direct inhibitory effect on phosphatase activity/PTP1B activity (for example, as detectable in assays in vitro). | 2013-01-31 |
20130029367 | COMPOSITIONS AND METHODS FOR MONITORING BREAST CANCER TREATMENT - The present invention provides compositions and processes for preparing the same wherein the compositions are useful for monitoring breast cancer treatment. | 2013-01-31 |
20130029368 | METABOLIC MATURATION IN STEM CELL-DERIVED TISSUE CELLS - The present invention concerns methods and compositions for promoting a conversion in stem cells or cells derived therefrom from glycolysis to aerobic respiration and its associated pathways, including oxidative phosphorylation, the TCA cycle, fatty acid oxidation, and pyruvate decarboxylation, for example. In specific embodiments, certain media is employed to improve metabolic maturation of particular stem cell-derived cells, including human embryonic stem cells and human induced pluripotent stem cells, for example. Particular media comprises media without glucose or optionally without pyruvate, with fatty acids, with L-carnitine, with taurine, creatine, non-essential amino acids, L-glutamine, and with anti-oxidants or free-radical scavengers, for example. | 2013-01-31 |
20130029369 | BIMODAL STAR POLYMER ARCHITECTURES AS FLUORESCENT AND MRI IMAGING REAGENTS - Disclosed are star polymers comprising a polymeric body having a core with a site-isolated chromophore and a plurality of polymer chains emanating from the core; and at least one chelating moiety bonded to at least one polymer chain. Also disclosed are bimodal contrast agents derived from star polymers and further comprising at least one metal chelated by the at least one chelating moiety. Also disclosed are methods of making and using same. Also disclosed are imaging methods employing the disclosed star polymers and/or bimodal contrast agents. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention. | 2013-01-31 |
20130029370 | System for Purifying Certain Cell Populations in Blood or Bone Marrow by Depleting Others - An apparatus and method for purifying and harvesting certain cell populations in blood or bone marrow by depleting at least one of red blood cells, granulocytes, or platelets from a sample comprising blood, bone marrow, or stromal vascular fraction cells separated from adipose tissue is disclosed. The apparatus comprises a sterile, single use rigid, self-supporting cartridge within which the automated depletion, purification and harvesting of target cell populations occurs and all components may be distributed. | 2013-01-31 |
20130029371 | DISK DIFFUSION ASSAY FOR ORITAVANCIN - The present invention is directed to a disk diffusion assay for determining susceptibility of bacteria to a glycopeptide antibiotic. The assay includes improvements over conventional assays due to the inclusion of polysorbate 80 and Span 80 in the antibacterial solution used to impregnate paper disks used in the assay. | 2013-01-31 |
20130029372 | B-CELL ANTIGEN PRESENTING CELL ASSAY - Disclosed herein are methods for diagnosing or predicting B-cell rejection in a subject. In one example, for assessing transplant rejection, the method includes determining an antigen presenting index by comparing uptake of a donor antigen to uptake of a reference antigen in a biological sample obtained from the subject. In another example, for assessing GVHD, the method includes determining an antigen presenting index by comparing uptake of a recipient antigen to uptake of a reference antigen in a biological sample obtained from the subject. | 2013-01-31 |
20130029373 | METHOD AND APPARATUS FOR ANALYZING INDIVIDUAL CELLS OR PARTICULATES USING FLUORESCENT QUENCHING AND/OR BLEACHING - A method for analyzing a blood sample is provided that includes the steps of: providing a blood sample having one or more of each first and second constituents; admixing a colorant with the sample, which colorant is operative to cause the first constituents and second constituents to fluoresce and absorb light; illuminating at least a portion of the sample; e) imaging a portion of the sample; determining a fluorescence value for each the first constituents and second constituents; determining an optical density value for each of the first constituents and second constituents; and identifying the first constituents and the second constituents using the determined fluorescence and optical density values. | 2013-01-31 |
20130029374 | METHOD AND MEASURING POINT FOR MEASURING AT LEAST ONE PHYSICAL AND/OR CHEMICAL, PROCESS VARIABLE OF A MEASURED MEDIUM CONTAINED IN A SINGLE USE CONTAINER - A method, and a measuring point for performing the method, for measuring at least one physical and/or chemical, process variable of a medium contained in a single use container. A single use sensor is connected with the single use container. The single use sensor with the single use container is sterilized by radiation, especially beta or gamma radiation. An electronics unit is connected with the single use sensor, and the electronics unit is connected with at least one superordinated system via at least one interface. The electronics unit and/or the single use sensor are/is supplied with energy, and measurement data of the single use sensor are registered. The measurement data are processed by the electronics unit and/or the superordinated system, data, including measurement data, are transmitted to the superordinated system and/or received from the superordinated system, and, after termination of one or more measuring cycles, the electronics unit is released from the single use sensor. | 2013-01-31 |
20130029375 | BIOLOGICAL FIXATIVE AND METHOD OF USING THE BIOLOGICAL FIXATIVE - A composition that includes an aldehyde, alcohol, and a ketone, the volumetric ratio of the alcohol to the ketone in the composition ranging from as low as about 0.8:1 to as high as about 4.5:1 and the volumetric ratio of the alcohol to the aldehyde in the composition ranging from as low as about 41.5:1 to as high as about 450:1. | 2013-01-31 |
20130029376 | METHODS AND COMPOSITIONS FOR A MICROEMULSION-BASED TISSUE TREATMENT - The invention is directed to methods and compositions for deparaffinizing paraffin-embedded biological samples for subsequent tissue staining The compositions are microemulsions that may include water/oil/surfactant microemulsions, and optionally a cosurfactant. The microemulsions enable deparaffinization without the use of xylene or toluene, and also enable solvent exchange without the use of intermediary alcohol dehydration or alcohol rehydration compositions. | 2013-01-31 |
20130029377 | RECOMBINANT APOA-1M FROM ENGINEERED BACTERIA - Apolipoprotein A-1 Milano (ApoA-1M), the protein component of a high-density lipoprotein (HDL) mimic with promising potential for reduction of atherosclerotic plaque, is produced on a large scale by expression in | 2013-01-31 |
20130029378 | NOVEL ENZYME - The invention provides an isolated formate dehydrogenase (FDH) polypeptide specific for NADP | 2013-01-31 |
20130029379 | VIRUS-BASED VECTOR COMPOSITIONS USEFUL FOR TRANSDUCING EUKARYOTIC CELLS - The present invention provides viral vector compositions of high titre and purity, as well as methods for production of said compositions. The methods of the invention incorporate multiple features, such as production of viral vector particles in serum free media and multiple harvesting steps following transduction of the producer cell which provides for enhanced production of said viral vectors. The viral vector compositions of the invention, by virtue of their high titre and purity, minimize the deleterious phenotypic changes that typically occur following transduction of target cells, such as loss of a sub-populations of transduced cells, and effects on proliferation, differentiation, reprogramming or functionality of transduced cells. | 2013-01-31 |
20130029380 | Asymmetric Adapter Library Construction - The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters. | 2013-01-31 |
20130029381 | MICROORGANISMS FOR THE PRODUCTION OF 1,4-BUTANEDIOL, 4-HYDROXYBUTANAL, 4-HYDROXYBUTYRYL-COA, PUTRESCINE AND RELATED COMPOUNDS, AND METHODS RELATED THERETO - The invention provides non-naturally occurring microbial organisms comprising a 1,4-butanediol (BDO), 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway comprising at least one exogenous nucleic acid encoding a BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine pathway enzyme expressed in a sufficient amount to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine and further optimized for expression of BDO. The invention additionally provides methods of using such microbial organisms to produce BDO, 4-hydroxybutyryl-CoA, 4-hydroxybutanal or putrescine. | 2013-01-31 |
20130029382 | COMPOSITIONS AND METHODS RELATING TO DUAL ACTIVITY ENZYMES HAVING XYLANASE AND CELLULASE ACTIVITY - Compositions and methods are provided for treating lignocellulosic material with a dual activity enzyme having xylanase and cellulase activity. The enzyme is stable and active at increased pH and increased temperatures. The present invention therefore provides methods for hydrolyzing lignocellulosic material, especially cellulose and hemicellulose, which are major components of the cell wall of non-woody and woody plants. The methods for hydrolyzing cellulose and hemicellulose can be used on any plant, wood or wood product, wood waste, paper pulp, paper product or paper waste or byproduct. | 2013-01-31 |
20130029383 | Method of Producing Hydroxymethyl-Furfural - The present invention relates to a method of producing 5-hydroxymethylfurfural by dehydration of fructose and/or glucose and/or mannose. | 2013-01-31 |
20130029384 | THERMOSTABLE SUCROSE PHOSPHORYLASE - The present invention relates to a sucrose phosphorylase from | 2013-01-31 |
20130029385 | Processes Using Amino Acid Dehydrogenases and Ketoreductase-Based Cofactor Regenerating System - The present disclosure relates to the use of an amino acid dehydrogenase in combination with a cofactor regenerating system comprising a ketoreductase. In particular embodiments, the process can be used to prepare L-tert-leucine using a leucine dehydrogenase. | 2013-01-31 |
20130029386 | COMPOSITIONS AND METHODS FOR THE PRODUCTION OF L-HOMOALANINE - Healthcare costs are a significant worldwide, with many patients being denied medications because of their high prices. One approach to addressing this problem involves the biosynthesis of chiral drug intermediates, an environmentally friendly solution that can be used to generate pharmaceuticals at much lower costs than conventional techniques. In this context, embodiments of the invention comprise methods and materials designed to allow microorganisms to biosynthesize the nonnatural amino acid L-homoalanine. As is known in the art, L-homoalanine is a chiral precursor of a variety of pharmaceutically valuable compounds including the anticonvulsant medications levetiracetam (sold under the trade name Keppra®) and brivaracetam, as well as ethambutol, a bacteriostatic antimycobacterial drug used to treat tuberculosis. Consequently, embodiments of the invention can be used in low cost, environmentally friendly processes to generate these and other valuable compounds. | 2013-01-31 |
20130029387 | MATERIALS AND METHODS FOR USING AN ACYL-ACYL CARRIER PROTEIN THIOESTERASE AND MUTANTS AND CHIMERAS THEREOF IN FATTY ACID SYNTHESIS - A method of increasing production of fatty acids comprising introducing into a host cell or organism and expressing therein an acyl-acyl carrier protein (ACP) thioesterase (TE) from | 2013-01-31 |
20130029388 | Method for Accumulation of Polyhydroxyalkanoates in Biomass with On-Line Monitoring for Feed Rate Control and Process Termination - A method or process for producing polyhydroxyalkanoates (PHAs) in biomass. The process entails feeding an organic carbon containing substrate to biomass enriched in PHA accumulating bacteria. Particularly the process entails intermittently supplying the substrate to the biomass at least three separate times over a selected period. The object of the process is to produce PHA having a relatively high molecular weight, at least 400,000 g/mole. By controlling the frequency at which the substrate is supplied to the biomass and by feeding a sufficient amount of the substrate to the biomass, the method or process produces the PHA having the relatively high molecular weight. | 2013-01-31 |
20130029389 | METHOD FOR PRODUCING D TYPE LACTIC ACID WITH HIGH OPTICAL PURITY - The present invention provides a method for producing D-type lactic acid by using | 2013-01-31 |
20130029390 | ENERGY PRODUCTION WITH HYPERTHERMOPHILIC ORGANISMS - The present invention relates to the field of degradation with hyperthermophilic organisms, and in particular to the use of hyperthermophilic degradation to produce heat and energy rich components including hydrogen and ethanol from a biomass. In some embodiments, a biomass is fermented in the presence of hyperthermophilic organisms to produce heat. The heat is used to heat a liquid which is used directly in a heat pump or radiant heat or to produce electricity or drive a steam turbine. In some embodiments, acetate is utilized as a substrate to produce energy by methanogenesis. | 2013-01-31 |
20130029391 | METHOD OF PREPARING KODA USING LEMNA PAUCICOSTATA - After identifying the genes of a novel lipoxygenase and a novel allene oxide synthase derived from Lemna paucicostata SH strain, a plant growth regulating agent (KODA) was produced at high yield by using a Lemna paucicostata strain that expresses the lipoxygenase and the allene oxide synthase. | 2013-01-31 |
20130029392 | METHOD FOR PRODUCING HYDROXYCARBOXYLIC ACID BY ENHANCING SYNTHESIS OF COENZYME - Hydroxycarboxylic acids are produced by using a microorganism that is improved in ability to produce nicotinamide adenine dinucleotide by deleting, mutating or substituting nadR gene in the microorganism or introducing a gene encoding nicotinic acid phosphoribosyltransferase. | 2013-01-31 |
20130029393 | RECOMBINANT ETHANOLOGENIC BACTERIA - The invention provides recombinant ethanologenic bacteria, methods of making the bacteria and methods of producing ethanol using the bacteria. | 2013-01-31 |
20130029394 | APPARATUS AND PROCESS FOR TREATING WASTE - A method for treating municipal solid waste and other waste is provided which comprises: introducing said waste into a rotary autoclave which is downwardly inclined towards its discharge end and has a door at the discharge end; and injecting steam through said door into said autoclave to treat the load. A method is also provided for treating waste, comprising steam autoclaving the waste, anaerobically digesting an organic-rich fraction of the autoclaved waste, recovering methane-containing gas from anaerobic digestion, internally combusting the methane-containing gas to generate power and exhaust gas, and generating steam for autoclaving using the waste heat. A plant for treating the waste may comprise at least one autoclave for steam treating the waste, at least one anaerobic digestion tank for digesting an organic-rich fraction of the autoclaved waste, a recovery system for recovering methane-containing gas from the or each digestion tank, at least one internal combustion engine for combusting the methane-containing gas and generating power, and a steam generator fed with combustion gas from the internal combustion engine for generating and accumulating steam for supply to said at least one autoclave. Also provided is a method of treating waste material in a rotary autoclave, which comprises: loading the waste material into a top opening of the autoclave whilst rotating the autoclave in a first direction in which screw flights within the autoclave convey the waste forwardly along a downwardly inclined body of the autoclave towards a base of the autoclave; rotating the autoclave in a second direction opposite to the first direction so as to establish a circulation of the loaded material between the upper and lower ends of the autoclave to facilitate vacuum and/or steam treatment thereof; and monitoring the load imparted by the autoclave adjacent upper and lower ends thereof during the reverse rotation, increase of the load adjacent the upper end of the autoclave providing an indication of effective load circulation. A door structure for a commercial-scale autoclave based on a castellated door and a locking ring with lock blocks of inwardly facing U-structure is also provided. | 2013-01-31 |
20130029395 | METHODS AND COMPOSITIONS FOR PRODUCING HYDROCARBONS - Compositions and methods for producing aldehydes, alkanes, and alkenes are described herein. The aldehydes, alkanes, and alkenes can be used in biofuels. | 2013-01-31 |
20130029396 | Multifunctional Biomemory Device - The present disclosure relates to a multifunctional biomemory device in which a protein having a redox potential substituted with a metal ion is directly immobilized on a substrate. The present disclosure provides an operating method in which the redox state of the protein is controlled by applying three different potentials. The present disclosure provides a biomemory device in which the metal ion of a metalloprotein is substituted to allow for artificial control of the redox potential. The present disclosure provides a new-concept biomemory device as an information storage device based on the principle of electron transfer of a naturally occurring biomolecule. | 2013-01-31 |
20130029397 | MATERIALS AND METHODS FOR PRODUCING CELL-SURFACE DIRECTED AND ASSOCIATED NON-NATURALLY OCCURRING BIOINORGANIC MEMBRANCES AND USES THEREOF - Materials and methods are provided for producing cell-surface directed, non-naturally occurring, bioinorganic membranes for association with the cell surfaces of living cells. The methods comprise exposing a cell to an acidic biomineralization buffer environment for cell-mediated deposition of the biomineral membrane onto the surface of the cell. The methods also comprise attaching a peptide, having a net positive charge under the acidic conditions, to the cell surface for serving as a template in directing the cell-mediated deposition of the biomineral membrane onto the surface of the cell. | 2013-01-31 |
20130029398 | FORMED ARTICLE AND PROCESS FOR THE PRODUCTION THEREOF - This invention is made to provide a formed article which ensures, for example, that an active substance included therein and a substance outside capsules can effectively come in contact with each other without suffering influences such as a pressure loss. This invention is a formed article having a plurality of cells formed in a polymer (A), (1) each cell including an active substance, (2) the polymer (A) having pores, each pore communicating with other pores and having a diameter in the range of 1 nm to 1 μm, and (3) the internal wall of each cell and the active substance being substantially not in contact with each other. | 2013-01-31 |
20130029399 | METAL SALEN COMPLEX DERIVATIVE AND PROCESS FOR PRODUCTION THEREOF - A metal-salen complex derivative with an excellent yield and stability is provided and a method for producing such a metal-salen complex derivative is provided. The present invention provides: metal-salen complex derivative obtained by allowing a target component composed of at least one of an enzyme, an antibody, an antigen, a peptide, an amino acid, an oligonucleotide, a protein, a nucleic acid, and a medical molecule to bind to a metal-salen complex via an amide bond or a disulfide bond; a method for producing such a metal-salen complex derivative. | 2013-01-31 |
20130029400 | GENETIC ELEMENTS, PROTEINS, AND ASSOCIATED METHODS INCLUDING APPLICATION OF ADDIITNAL GENETIC INFORMATION TO GRAM (+) THERMOACIDOPHILES - Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from | 2013-01-31 |
20130029401 | Synthetic Binding Proteins - Methods and compositions are provided for creating a binding protein that recognizes a rationally chosen recognition sequence in which a first amino acid has been substituted for a second amino acid using site-directed mutagenesis of a member protein of a set of proteins at an identified position or positions correlated with recognition of a chosen specified target module in the recognition sequence. A system is provided for automating the storage and manipulation of the correlations between positions and types of amino acid residues in the binding protein with specific modules at specified positions in the target recognition sequence and for designing and creating proteins with novel specificities. | 2013-01-31 |
20130029402 | BACILLUS STRAIN FOR INCREASED PROTEIN PRODUCTION - The present invention provides host cells that have been genetically manipulated to have an enhanced capacity to produce proteins of interest. In particular, the invention relates to modified | 2013-01-31 |
20130029403 | System and Method for Using a Pulse Flow Circulation for Algae Cultivation - A system and method for using a pulse flow to circulate algae in an algae cultivation apparatus are provided. In order to counteract the negative effects of biofouling on algae cultivation equipment, a pulse flow is created to periodically move through an algae cultivation apparatus. The pulse flow will dislodge algae cells adhering to various surfaces of the apparatus, and it will also create turbulence to stir up any algae cells which may have settled onto the bottom of the apparatus. To produce an increased fluid flow rate required to create an effective pulse flow, a sump, which is periodically filled with drawn algal culture from the apparatus, is located at an elevated position above the apparatus. When released, the algal culture travels through a transfer pipe and into the apparatus with gravity causing the algal culture to flow at a very high rate. | 2013-01-31 |
20130029404 | PHOTOBIOREACTOR IN A CLOSED ENVIRONMENT FOR CULTIVATING PHOTOSYNTHETIC MICRO-ORGANISMS - The invention relates to a photobioreactor for cultivating photosynthetic micro-organisms, comprising: a) at least one cultivation container ( | 2013-01-31 |
20130029405 | AMMONIUM RECOVERY METHODS - The methods are utilized to recover ammonium from waste water using CO | 2013-01-31 |
20130029406 | TWO STAGE CONTINUOUS PRE-TREATMENT OF LIGNOCELLULOSIC BIOMASS - Disclosed is a method of pretreating biomass in two pretreatment stages as part of a biofuel production process. The first stage pretreatment is carried out by heating the biomass to a first stage temperature of 140° C. to 180° C. for a first stage time of 30 minutes to 2 hours at a first stage pressure of 105 to 150 psig; and the second stage is carried out by heating the biomass to a second stage temperature of 190° C. to 210° C. for a second stage time of 2 to 10 minutes at a second stage pressure of 167 to 262 psig. The biomass may be initially conditioned prior to the first pretreatment stage by atmospheric steam heating and adjusting the moisture content of the biomass. Hemicellulose and inhibitors (inhibitory compounds) to downstream hydrolysis and fermentation are preferably removed between the first and second pretreating stages, more preferably after each pretreatment stage. | 2013-01-31 |
20130029407 | CELL ANALYZER - Provided is a cell concentration and purification device, having: a function of continuously concentrating cells; a function of then subsequently disposing the cells continuously in a specific region of a channel; a function of simultaneously recognizing, based on an image, the shape and fluorescence emission of each single cell; and a function of recognizing the cells and then separating and purifying the same based on the data relating to the shape and fluorescence emission thereof. | 2013-01-31 |
20130029408 | INTEGRATED SYSTEM FOR SAMPLING AND ANALYSIS - There is disclosed a device for determination of a sample from a dielectric fluid medium for a bio-specific assay device, comprising: a sampling device and a biosensor, wherein the sampling device concentrates a sample from dielectric medium by electrically focusing the sample on to a capture element and wherein said biosensor is fluidically linked to said capture element thus providing sampling and determination in a unitary device. | 2013-01-31 |
20130029409 | METHOD AND APPARATUS FOR AUTOMATIC STAINING OF TISSUE SAMPLES - The present invention concerns an apparatus for staining tissue samples, said apparatus including a reagent section or reagent containers; at least one staining section or tissue samples, a robotic head or robotic element that may move reagent to a predetermined tissue sample, said robotic element being moveable above the reagent and the staining sections, a control element that may manage a staining process, a 2-D optical sensor to detect two-dimensional image data of a relevant property and that can feed the captured image data to the control element. By providing the robotic element with a 2-D optical sensor, a common image processor may be provided having multiple functions. By using a 2-D optical image processing system, the control system of the apparatus may easily be adapted to read various types of data presentations, just as actual images for sections of the apparatus may be identified in order to assess the condition of the apparatus. The optical sensor may be used to automatically identify the slides and the reagent containers present in the apparatus, just as the optical sensor may be used for checking if a slide is misplaced at or absent from a slide position, etc. | 2013-01-31 |
20130029410 | DIGESTER TANK FOR A BIOGAS PLANT - The invention relates to a digester tank for a biogas plant. The interior of the digester tank is at least partially surrounded by media-tight, pressure-tight and thermally insulating walls. The digester tank has thermally insulating exterior wall segments. The thermally insulating exterior wall segments form a self-supporting polygonal ring. | 2013-01-31 |
20130029411 | SYSTEMS, METHODS AND CONTROL LAWS FOR CELL HARVESTING - The methods of harvesting cells are provided, wherein the methods comprise introducing a processing material and a source material into a processing loop. The processing loop comprises a processing chamber and a filtering device. The processing material and the source material are circulating through the processing chamber and the filtering device, wherein the processing chamber has a mass; balancing an influx of the processing material into the processing chamber with a permeate flux of the filtering device to maintain the mass of the processing chamber at a constant value; and collecting the cells in a collection chamber. Cell harvesting devices are also provided for processing and harvesting cells using a control law to balance the mass of the processing chamber through the entire process. | 2013-01-31 |
20130029412 | CELL CULTURE SYSTEM - A microtiter plate based cell culture system comprising an improved cell culture insert which is suitable and used for the cultivation of adherent cells and/or three-dimensional tissues. | 2013-01-31 |
20130029413 | Methods of Using a Bacterial GlcNAc-6-P 2'- Epimerase to Promote Sialylation of Glycoconjugates - The present invention relates to new methods to promote sialylation of glycoconjugates, including recombinant glycoproteins, in glycoconjugate production systems. The invention relates to methods to promote efficient glycoconjugate sialylation in recombinant expression systems, by providing simpler and more economical ways to produce large intracellular pools of sialic acid precursors. The invention is directed to nucleic acids, vectors, and cells harboring vectors comprising nucleic acids encoding enzymes involved in the synthesis of sialic acid precursors, and cells harboring these nucleic acids in combination with nucleic acids encoding glycosyltransferases, including sialyltransferases, to facilitate the production of humanized recombinant glycoproteins in bacterial, fungal, plant, and animal cell expression systems. The engineered cells can be used to produce glycosylated proteins in virally-infected, transiently-transformed, or stably-transformed host cells, including lepidopteran insects and cultured cell lines derived from | 2013-01-31 |
20130029414 | CELL CULTURE MEDIA, KITS AND METHODS OF USE - Albumin-supplemented and xenogeneic product-free cell culture media, cell culture media supplements, and cell culture media kits for the support of primary culture of normal non-hematopoietic cells of mesodermal origin suitable for both research and clinical applications. | 2013-01-31 |
20130029415 | CELL CULTIVATION METHOD AND CELL CULTURE - Provided is a cell cultivation method in which the cell is cultured using a peptide hydrogel as a scaffold, for carrying out high-dimensional culture of a cell such as porcine hepatocyte, human hepatocyte, porcine pancreatic islet or human pancreatic islet for a long period under conditions where cell survival, cell morphology and cell functions are maintained. Also provided are a cell culture including a cell and a peptide hydrogel obtained by the above-described cultivation method, a bioreactor including the cell culture, and a cell preparation including the cell culture. | 2013-01-31 |
20130029416 | DIFFERENTIATING INDUCED PLURIPOTENT STEM CELLS INTO GLUCOSE-RESPONSIVE, INSULIN-SECRETING PROGENY - This document provides methods and materials related to differentiating iPS cells into glucose-responsive, insulin-secreting progeny. For example, methods and material for using indolactam V (ILV) and glucagon like peptide-1 (GLP-1) to produce glucose-responsive, insulin-secreting progeny from iPS cells are provided. | 2013-01-31 |
20130029417 | Suppressors of RNA Silencing as Modulators of miRNA Levels - The invention describes use of RNA silencing suppressors or interactors of the suppressors to bring the expression of microRNAs involved in any disease, including malignant neoplasia, back to its normal level. More specifically the present invention provides a method to regulate many miRNAs at the same time. Most of the suppressors according to this invention are coded by plant viruses that unexpectedly can affect RNA silencing and modulate miRNA expression levels in mammalian cells. Also suppressors of endogenous origin are described as able to modulate miRNA expression levels. | 2013-01-31 |
20130029418 | METHODS AND PRODUCTS FOR TRANSFECTION - The present invention relates in part to methods for producing tissue-specific cells from patient samples, and to tissue-specific cells produced using these methods. Methods for reprogramming cells using RNA are disclosed. Therapeutics comprising cells produced using these methods are also disclosed. | 2013-01-31 |