05th week of 2014 patent applcation highlights part 45 |
Patent application number | Title | Published |
20140030684 | ACTIVITY REGULATION BASED ON BIOMETRIC DATA - Disclosed are devices, systems, apparatus, methods, products, and other implementations, including a method that includes obtaining biometric data of a user, and generating instruction data, presentable on a user interface, based on data relating to one or more activities to be completed by the user and based on the biometric data of the user. In some embodiments, obtaining the biometric data may include measuring one or more of, for example, heart rate, blood pressure, blood oxygen level, temperature, speech-related attributes, breath, and/or eye behavior. | 2014-01-30 |
20140030685 | TOOTH FOR DENTAL ARCH MODEL AND METHOD FOR PRODUCING THE SAME - The present invention relates to a tooth which is used in a dental arch model with which dental students can experience dental works in the oral cavity and practice treatments. More specifically, the present invention relates to a tooth composition for formation trainings such as abutment tooth formation and cavity preparation. Since conventionally used dental arch models have a grinding sensation different from a natural tooth, dental students having completed formation trainings such as abutment tooth formation and cavity preparation by using these conventional models are frequently puzzled to have different grinding and handling properties upon dental works in the oral cavity in practice. That is, these conventional dental arch models are largely different from a natural tooth in grinding sensation including slipperiness and easiness in grinding. A tooth for a dental arch model simulating the enamel texture and the dentin texture constituting a natural tooth whereby even the difference in grinding sensation between the enamel texture and the dentin texture constituting a natural tooth can be reproduced by forming at least the enamel portion with the use of a sintered body of an inorganic powder such as alumina. | 2014-01-30 |
20140030686 | CURRICULUM ASSESSMENT - One or more embodiments of techniques or systems for curriculum assessment are provided herein. One or more assessments can be generated. For example, an assessment can be generated based on one or more forms. In this way, assessments can be created in a structured manner and distributed accordingly. One or more of the assessments may be administered. After one or more students or test takers take the assessments, one or more of the assessments can be analyzed or aggregated. Analysis can include statistical analysis related to one or more similar, related, or associated assessments. In this manner, curriculum assessment can be provided, thereby enhancing teaching efficiency and productivity, etc. | 2014-01-30 |
20140030687 | INCLUDING USAGE DATA TO IMPROVE COMPUTER-BASED TESTING OF APTITUDE - Administration of an aptitude test is limited to one or more explicitly authorized computers associated with the user and usage of each computer is monitored during administration of the test and evaluated to make inferences regarding the user's aptitude beyond the direct results of the test. If the computer used by an authenticated is not properly authorized for the user, much tighter authentication is required to add the computer as an authorized computer. In addition, the server determines an approximate geological location of the computer. If the computer is determined to be at a location the user is not expected to be, the server refuses to administer the test. The server receives the responsive solutions provided by the user along with usage data representing usage of the user's computer during the pendency of each challenge. In evaluating the test results, the usage data is used to make one or more inferences of the user's aptitude. | 2014-01-30 |
20140030688 | SYSTEMS, METHODS AND PROGRAM PRODUCTS FOR COLLECTING AND DISPLAYING QUERY RESPONSES OVER A DATA NETWORK - One method for communicating recommendations over a data network, comprising the steps of an initiating participant asking a question of a primary responder over the network; the primary responder providing a first answer to the question over the network and recommending at least one secondary responder to provide an answer to the question; each of the at least one secondary responder providing a second answer to the question over the network and further recommending at least one tertiary responder to provide an answer to the question; the at least one tertiary responder providing a third answer to the question over the network; and, a computer receiving each of the first, second and third answers and displaying them in a hierarchical manner. | 2014-01-30 |
20140030689 | TESTING TIMER AND TESTING ANALYTICS - Embodiments described herein disclose methods and systems to automatically present a checklist element to be completed by the medical practitioner. In embodiments, the medical practitioner may indicate that the presented checklist element is completed without using their hands. Therefore, the medical practitioner may maintain a sterile environment while completing checklist elements reducing the amount of time that the medical practitioner uses to complete a medical procedure. | 2014-01-30 |
20140030690 | Systems and Methods for Testing Over a Distributed Network - A computer-based testing system includes testing stations connected to a testing service center and backend via the Internet for providing testing services. The system is operable to perform state management to implement fault recovery due to a computing device failure while a test is being administered. The system is also operable to utilize multiple caching techniques for mitigating network latency while administering tests. | 2014-01-30 |
20140030691 | System and Method for Collaborative Electronic Textbook Authoring, Instructional Material Generation, Coursework Distribution, and Feedback-Based Content Adaptation - A computer-based process for managing electronic textbook (e-textbook) content, generation, distribution, and usage includes receiving inputs corresponding to first e-textbook content from at least one e-textbook author by way of an author interface in communication with a content authoring engine; generating a first e-textbook corresponding to the first e-textbook content in accordance with an e-textbook taxonomy; generating instructional materials corresponding to portions of the first e-textbook in accordance with an instructional materials taxonomy; communicating at least one of first e-textbook content and instructional materials to at least one e-learning application; and receiving feedback by way of a feedback engine, wherein the feedback corresponds to at least one of (a) how instructional materials are utilized by at least one instructor, (b) how instructional materials or first e-textbook content are utilized by students, and (c) a recommendation for revising first e-textbook content in a subsequent edition of the first e-textbook. | 2014-01-30 |
20140030692 | APERTURE FOR NIGHT VISION GOGGLES FOR USE IN AIRCRAFT SIMULATOR OR OTHER SIMULATORS - An aperture mechanism for night vision goggles (NVG) has a first end, a second end, a lens engaging portion, and a cone portion. A lens engaging portion has a recession allowing attachment of the aperture mechanism to a light admitting end of the NVG. A cone portion is opposite the lens engaging portion. Together the lens engaging portion and the cone portion form an aperture. The aperture mechanism increases the depth of field of the NVG for applications involving small object-to-goggle distances, such as in simulators or simulator environments. In a simulator system, the aperture mechanism can be attached to NVG such that at least a portion of light from a display screen is blocked. The light output of the display screen can be increased such that the depth of field remains higher but the image produced does not become darker. | 2014-01-30 |
20140030693 | METHOD AND DEVICE FOR REAL TIME EXPRESSION - Methods and devices for real time expression at an event. In one embodiment, an article includes an image of a figure, entity, or individual and an expression area including a rewritable surface. In another embodiment, a method includes the steps of displaying an article including an image of a figure, entity, or individual, where the article also includes an expression area with a rewritable surface, displaying the article in a public or private event, where a figure, entity, or individual is participating in the event, and expressing a sentiment in the expression area. | 2014-01-30 |
20140030694 | OXYGEN DEPLETION DEVICES AND METHODS FOR REMOVING OXYGEN FROM RED BLOOD CELLS - An oxygen depletion device. The device has a cartridge; a plurality of hollow fibers extending within the cartridge from an entrance to an exit thereof; an amount of an oxygen scavenger packed within the cartridge and contiguous to and in between the plurality of hollow fibers. The hollow fibers are adapted to receiving and conveying red blood cells. There is another embodiment of an oxygen depletion device and method for removing oxygen from red blood cells. | 2014-01-30 |
20140030695 | Biorefinery Control System, Components Therefor, and Methods of Use - An autonomous control system can be used with one or more systems or components to improve the operation of the systems or components. For example, an autonomous control system can be used in combination with a biorefinery system to improve the operation of the biorefinery system. | 2014-01-30 |
20140030696 | METHODS, SYSTEMS AND APPARATUS FOR PERFORMING FLOW CYTOMETRY - An apparatus for detecting an analyte in a sample includes an illumination source for generating electromagnetic energy to illuminate the sample at an interrogation region, a concave collector element having an optical axis, and a focal point, the interrogation region being coincident with the focal point of the concave collector element, a closed flow cell having a flow path defined between a sample inlet and a sample outlet, the flow path passing through the interrogation region and a sorting region disposed downstream of the interrogation region. The portion of the flow path passing through the interrogation region is coaxially aligned with the optical axis of the concave collector element. The apparatus further includes the sample comprising or suspected of comprising the analyte and flowing in the flow path, wherein the analyte generates a detectable signal in response to illumination. The apparatus also includes a detector for detecting the detectable signal. | 2014-01-30 |
20140030697 | SORTASE-MEDIATED MODIFICATION OF VIRAL SURFACE PROTEINS - The present invention, in some aspects, provides methods, reagents, and kits for the functionalization of proteins on the surface of viral particles, for example, of bacteriophages, using sortase-mediated transpeptidation reactions. Some aspects of this invention provide methods for the conjugation of an agent, for example, a detectable label, a binding agent, a click-chemistry handle, or a small molecule to a surface protein of a viral particle. Kits comprising reagents useful for the generation of functionalized viral particles are also provided, as are precursor proteins that comprise a sortase recognition motif, and viral particles comprising such precursor proteins. Nucleic acids encoding viral proteins comprising a sortase recognition motif and expression vectors comprising such nucleic acids are also provided. | 2014-01-30 |
20140030698 | MULTIPLEX ASSAY FOR THE DETECTION OF CITRUS PATHOGENS - The present invention provides methods and compositions for detecting multiple citrus pathogens using a multiplex branched DNA signal amplification reaction. | 2014-01-30 |
20140030699 | ELECTRICALLY CONDUCTIVE POLYMER ELECTRODES WITH INCORPORATED VIRUSES - Grafting M13 bacteriophage into an array of poly(3,4-ethylenedioxythiophene) (PEDOT) nanowires generated hybrids of conducting polymers and replicable genetic packages (rgps) such as viruses. The incorporation of rgps into the polymeric backbone of PEDOT occurs during electropolymerization via lithographically patterned nanowire electrodeposition (LPNE). The resultant arrays of rgps-PEDOT nanowires enable real-time, reagent-free electrochemical biosensing of analytes in physiologically relevant buffers. | 2014-01-30 |
20140030700 | ASSAYS FOR PATHOGEN DETECTION USING MICROWAVES FOR LYSING AND ACCELERATING METAL-ENHANCED FLUORESCENCE - The present invention relates to an assay for the determination of a microorganism including a lysing chamber having triangular shaped metallic structures wherein the apexes of two triangles are arranged in alignment and forming a reactive zone for placement of the microorganism and lysing by microwave energy for exposing and isolating a target polynucleotide sequence. The isolated target polynucleotide sequence is introduced to an assay system for contact with polynucleotides which are complimentary to the isolated target polynucleotide sequence. Fluorophore-labeled capture polynucleotides are added for hybridizing to any bound target polynucleotide. Bound target polynucleotides are detected by metal enhanced fluorescence. | 2014-01-30 |
20140030701 | COMPOSITIONS, REACTION MIXTURES AND METHODS FOR DETECTING NUCLEIC ACIDS FROM TYPE A1 AND/OR TYPE C1 HUMAN PAPILLOMAVIRUS - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 2014-01-30 |
20140030702 | MODIFIED INFLUENZA VIRUS FOR MONITORING AND IMPROVING VACCINE EFFICIENCY - The immunogenicity of the influenza virus hemagglutinin (HA) molecule may be increased by substitutions of amino acids in the HA sequence. The substitution of specific HA residues, such as asparagine at position 223 of H5 HA, increase the sensitivity of the hemagglutinin inhibition (HI) assay by altering receptor specificity and/or antibody-antigen binding. HA molecules containing such substitutions will be useful in the development of diagnostic reference viruses and improved influenza vaccines. | 2014-01-30 |
20140030703 | Compositions and Methods for Detecting and Identifying Nucleic Acid Sequences in Biological Samples - The invention is directed to compositions and methods for isolating, detecting, amplifying, and quantitating pathogen-specific nucleic acids in a biological sample. The invention also provides diagnostic kits containing specific amplification primers, and labeled detection probes that specifically bind to the amplification products obtained therefrom. Also disclosed are compositions and methods for the isolation and characterization of nucleic acids that are specific to one or more pathogens, including for example Influenza virus and | 2014-01-30 |
20140030704 | Asymmetric Adapter Library Construction - The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters. | 2014-01-30 |
20140030705 | SYSTEMS AND METHODS FOR ASSESSING BIOMOLECULE CHARACTERISTICS - Provided are methods and systems for assessing the presence and extent of damage on a polynucleotide. The methods include incorporating a label at the site of the damage and imaging the label to determine the presence and extent of the damage. The systems include devices capable of performing damage assessment on single molecules. | 2014-01-30 |
20140030706 | METHODS OF IDENTIFYING A CELLULAR NASCENT RNA TRANSCRIPT - Methods and compositions for identifying a cellular nascent RNA transcript are provided. | 2014-01-30 |
20140030707 | SMALL RNA-DEPENDENT TRANSLATIONAL REGULATORY SYSTEM IN CELL OR ARTIFICIAL CELL MODEL - An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5′ to the ribosome-binding site, and a nucleotide sequence located 5′ to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3′ to the start codon, and a nucleotide sequence located 3′ to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein. | 2014-01-30 |
20140030708 | TRANSLATION-COUPLING SYSTEMS - Disclosed are systems and methods for coupling translation of a target gene to a detectable response gene. A version of the invention includes a translation-coupling cassette. The translation-coupling cassette includes a target gene, a response gene, a response-gene translation control element, and a secondary structure-forming sequence that reversibly forms a secondary structure masking the response-gene translation control element. Masking of the response-gene translation control element inhibits translation of the response gene. Full translation of the target gene results in unfolding of the secondary structure and consequent translation of the response gene. Translation of the target gene is determined by detecting presence of the response-gene protein product. The invention further includes RNA transcripts of the translation-coupling cassettes, vectors comprising the translation-coupling cassettes, hosts comprising the translation-coupling cassettes, methods of using the translation-coupling cassettes, and gene products produced with the translation-coupling cassettes. | 2014-01-30 |
20140030709 | Biomarker for Detecting High-Altitude Adaptation and High-Altitude Pulmonary Edema - Present invention relates to the Biomarkers for detecting high altitude adaptation and hypoxia responsiveness and the method thereof. The invention specifically relates to the Gene variants SNPIDs rs479200 and rs480902 in the first intron of EGLN1 (Prolyl Hydroxylase 2) gene as biomarkers for adaptation to high altitude and predisposition for high altitude pulmonary edema and hypoxia responsiveness using a novel integrative approach of phenotyping concepts of Ayurveda with population genetics, and disease genomics. More specifically, the C allele of SNP ID rs480902 and T allele of rs479200 of EGLN1 gene is more frequent in patients of HAPE and nearly absent in native highlanders. The present invention also provides primers and methods suitable for the detection of these allelic variants for the prediction of individual's adaptability to high altitude and hypoxia and/or the genetic analysis of the EGLN1 gene in a population. | 2014-01-30 |
20140030710 | GARDNERELLA VAGINALIS ASSAY - The present invention relates to nucleic acid amplification assays for the detection of nucleic acid sequences of | 2014-01-30 |
20140030711 | METHODS AND BIOMARKERS FOR DETECTION OF LYMPHOMA - The present invention relates to methods and biomarkers for detection and characterization of lymphoma (e.g., splenic marginal zone lymphoma) in biological samples (e.g., tissue samples, blood samples, plasma samples, cell samples, serum samples). | 2014-01-30 |
20140030712 | GENOMIC APPROACH TO THE IDENTIFICATION OF BIOMARKERS FOR ANTIBIOTIC RESISTANCE AND SUSCEPTIBILITY IN CLINICAL ISOLATES OF BACTERIAL PATHOGENS - In certain embodiments, the present invention concerns genotypic identification of bacteria that are resistant to a bacteria and subsequent determination of an appropriate therapy. In specific embodiments, a high-throughput genotypic detection method for biomarkers for antibiotic resistance and susceptibility allows efficient prescription practice and increases the likelihood of a successful therapeutic outcome. In certain embodiments, the information from the genotypic detection method is utilized for determining antibiotics that should be avoided or, alternatively, employed. | 2014-01-30 |
20140030713 | FILLER FOR ION EXCHANGE CHROMATOGRAPHY AND METHOD FOR SEPARATING AND DETECTING NUCLEIC ACID STRAND - The present invention aims to provide a filler for ion exchange chromatography which can sufficiently detect nucleic acid chains that differ in sequence of bases or nucleic acid chains that differ by a single base substitution. The present invention also aims to provide a method for separating and detecting a nucleic acid chain using the filler for ion exchange chromatography. The present invention relates to filler for ion exchange chromatography, comprising base fine particles, each particle having a strong cationic group and a weak cationic group on the surface thereof. | 2014-01-30 |
20140030714 | Method and means for distinguishing malignant from benign tumor samples, in particular in routine air dried fine needle aspiration biopsy (FNAB) - The invention concerns a method and means for distinguishing malignant from benign tumor samples of the thyroid, by performing a RNA extraction in a standard fine needle aspiration biopsy (FNAB) sample, in particular an air dried FNAB smear. The presence of gene-rearrangements and/or the expression of miRNA is analyzed in the isolated RNA, wherein the presence of a gene-rearrangement and/or the differential expression of miRNA is indicative for a malignant tumor. | 2014-01-30 |
20140030715 | METHOD OF USING BOTH MIR-196A AND MIR-196B AS BIOMARKERS FOR DETECTING ORAL CANCER - A method of using miR-196a and miR-196b as biomarkers for oral cancer detection is provided with the steps of analyzing a sample from each of a plurality of human beings in terms of miR-196a and miR-196b wherein the miR-196a has a sequence of SEQ ID NO: 1 and miR-196b has a sequence of SEQ ID NO: 2; and detecting one of the human beings to have oral cancer if intensity of either miR-196a or miR-196b of the sample belonging to the human being is higher than a predetermined value. | 2014-01-30 |
20140030716 | Novel tumor marker, its monoclonal antibodies and usage - The present invention relates to a novel tumor marker, DEP domain containing 6 (DEPDC6) protein, and its monoclonal antibodies. The present invention further provides the use of DEPDC6 protein and its monoclonal antibodies for the identification and/or treatment of cancer, such as liver cancer. | 2014-01-30 |
20140030717 | VALVELESS MICROFLUIDIC DEVICE - Provided are methods, devices and systems for analyte detection using a microfluidic device. | 2014-01-30 |
20140030718 | METHOD OF DETECTING AND IDENTIFYING CIRCULATING ANTIGENS IN HUMAN BIOLOGICAL SAMPLES - Disclosed herein is a method of detecting and identifying antigens that are shed into human bodily fluids during infection. The disclosed method allows circulating antigens associated with a particular infection to be detected within minutes or hours from testing as compared to days required with the current methods. Methods of identifying diagnostic indicators/targets for a given condition or disease are disclosed which include immunizing a veterinary subject with biological fluids obtained from a human infected with particular antigens to identify diagnostic targets for immunoassay. Also disclosed are methods of diagnosing and monitoring a | 2014-01-30 |
20140030719 | METHOD FOR ENHANCING EFFICIENCY AND SENSITIVITY IN NUCLEIC ACID AMPLIFICATION FROM BIOLOGICAL MATERIALS USING IONIC LIQUIDS - A method for amplifying a nucleic acid amplification in the presence of an ionic liquid that suppresses an inhibitor of nucleic acid amplification, particularly when in a biological material, and a composition useful for performing the method. | 2014-01-30 |
20140030720 | DUAL REFERENCE CALIBRATION METHOD AND SYSTEM FOR QUANTIFYING POLYNUCLEOTIDES - Method and system for quantifying target nucleic acids using real-time amplification and internal calibration adjustment. The invention employs dual reference calibration curves for approximating a complete calibration curve from only a single adjustment calibrator amplified on the instrument that is to be calibrated. | 2014-01-30 |
20140030721 | Exonuclease Enabled Proximity Extension Assays - The present invention relates to a proximity probe based detection assay (“proximity assay”) for an analyte in a sample, specifically a proximity probe extension assay (PEA), an in particular to an improvement in the method to reduce non-specific “background” signals, wherein the improvement comprises the use in such assays of a component comprising 3′ exonuclease activity, said method comprising: (a) contacting said sample with at least one set of at least first and second proximity probes, which probes each comprise an analyte-binding domain and a nucleic acid domain and can simultaneously bind to the analyte; (b) allowing the nucleic acid domains of the proximity probes to interact with each other upon binding of said proximity probes to said analyte, wherein said interaction comprises the formation of a duplex; (c) contacting said sample with a component comprising 3′ exonuclease activity; (d) extending the 3′ end of at least one nucleic acid domain of said duplex to generate an extension product, wherein the step may occur contemporaneously with or after step (c); and (e) amplifying and detecting the extension product. | 2014-01-30 |
20140030722 | NLRP7-BASED DIAGNOSIS OF FEMALE REPRODUCTIVE CONDITIONS - Methods, reagents and kits are described for the diagnosis of a female reproductive condition such as reproductive wastage, based on the detection of an alteration in a NLRP7-encoding nucleic acid or a NLRP7 polypeptide, relative to a corresponding wild-type NLRP7-encoding nucleic acid or NLRP7 polypeptide. | 2014-01-30 |
20140030723 | Method for Predicting the Response to a Therapy - A test kit for selecting a therapy for a steroid resistant patient presenting inflammatory symptoms comprises primer pairs or antibodies specific for at least one marker gene or protein translated from at least one marker gene, and instructions for use. The primer pairs or antibodies enable analysis of expression level of the at least one marker gene or translated protein from the at least one marker gene. | 2014-01-30 |
20140030724 | METHOD OF IDENTIFYING, ISOLATING AND/OR CULTURING FOETAL ERYTHROBLASTS - There is provided a method of identifying at least one foetal erythroblast in a sample, the method comprising analysing the morphology of at least one cell in the sample; wherein at least one analysed cell that is nucleated, is CD45 negative and comprises a relatively high cytoplasmic to nuclear ratio is identified as the foetal erythroblast. | 2014-01-30 |
20140030725 | MUTANT PROTEASE BIOSENSORS WITH ENHANCED DETECTION CHARACTERISTICS - A polynucleotide encoding a biosensor polypeptide comprising a modified circularly-permuted thermostable luciferase and a linker linking the C-terminal portion of the thermostable luciferase to the N-terminal portion of the thermostable luciferase. The modified circularly-permuted thermostable luciferase is modified relative to a parental circularly-permuted thermostable luciferase. The linker contains a sensor region capable of interacting with a target molecule in a cell. The modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the parental circularly-permuted thermostable luciferase in the presence of the target molecule. Alternatively, the modified circularly-permuted thermostable luciferase has an enhanced response after interaction of the biosensor with the target molecule relative to the modified circularly-permuted thermostable luciferase in the absence of the target molecule. | 2014-01-30 |
20140030726 | METHODS FOR IDENTIFYING MODULATORS OF MURINE DOUBLE MINUTE 2 - The disclosure is directed to methods of identifying compounds that modulate murine double-minute 2. More particularly, the disclosure is directed to methods of identifying compounds that modulate murine double-minute 2 neddylation activity. | 2014-01-30 |
20140030727 | LOSS OF 5-HYDROXYMETHYLCYTOSINE AS A BIOMARKER FOR CANCER - Methods for detecting or diagnosing cancer in a subject are provided herein. Such methods may include, but are not limited to, measuring a test level of 5hmC in a biological sample from the subject; and determining that the subject has a malignant cancer when the test level of 5hmC is lower than that of a control level of 5hmC. Such methods may further include a step of measuring a test level of Ki67 in the biological sample and determining that the subject has a malignant cancer when the test level of Ki67 is higher than that of a control level of Ki67. | 2014-01-30 |
20140030728 | METHOD AND APPARATUS FOR ANALYZING CELLS - A method of analyzing cells, the method comprising: measuring a number of cells which are nuclear stained, in a to-be-determined tissue, and acquiring a histogram showing a fluorescence intensity based on a result of the measurement; analyzing the histogram, and acquiring data of predetermined parameters; comparing the data of the parameters with first thresholds predetermined for the parameters, to perform a first cancer determination for each of the parameters on the to-be-determined tissue; performing a scoring process for each of the parameters, on a result of the first cancer determination for each of the parameters, to calculate scores of the parameters; and combining the scores of the parameters with one another, thereby performing a second cancer determination on the to-be-determined tissue. | 2014-01-30 |
20140030729 | DETECTION OF CIRCULATING TUMOR CELLS USING IMAGING FLOW CYTOMETRY - An automated identification of the types of white blood cells in a blood sample facilitates the manual identification of cancerous or other abnormal blood cells in the sample. Classifiers are predetermined for each type of white blood cell and subsequently used to automatically process images of cells in a sample stained with a nuclear dye or stain. The classifiers each comprise a linear weighted combination of morphometric and photometric features previously selected for white blood cells that were identified using monoclonal antibody stains. Red blood cells and excess fluid are removed from a sample being processed upstream of an imaging region of the imaging system. A plurality of different types of images are produced for each cell by the imaging system enabling automated identification of the white blood cells. Images of any cells not thus identified are manually reviewed to detect cancerous or abnormal cells. | 2014-01-30 |
20140030730 | ACETYL-COA PRODUCING ENZYMES IN YEAST - The present invention relates to a method of identifying a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) a yeast cell comprising: a) providing a mutated yeast cell comprising a deletion of at least one gene of the (PDH) by-pass, selected from the genes encoding the enzymes pyruvate decarboxylase (PDC), acetaldehyde dehydrogenase (ALD), and acetyl-CoA synthetase (ACS); b) transforming said mutated yeast cell with an expression vector comprising a heterologous nucleotide sequence encoding a candidate polypeptide having potential enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA; c) testing said recombinant mutated yeast cell for its ability to grow on minimal medium containing glucose as sole carbon source, and d) identifying said candidate polypeptide as a heterologous polypeptide having enzymatic activity for converting pyruvate, acetaldehyde or acetate into acetyl-CoA in (the cytosol of) said yeast cell when growth of said cell is observed. The invention further relates to a method of producing a fermentation production such as butanol. | 2014-01-30 |
20140030731 | REGENTS FOR PROBING ROCKS ACTIVATION - A set of agents is provided for probing/detecting the activation of Rho-associated protein kinase I and II individually encoding their amino acid sequence (SEQ ID NO:1 for ROCKI and SEQ ID NO:2 for ROCKII) in biological samples. The agents include one antibody to specifically recognize ROCKI Ser | 2014-01-30 |
20140030732 | MICROFLUIDIC HPLC-CHIP FOR GLYCOPEPTIDE ANALYSIS WITH INTEGRATED HILIC ENRICHMENT - A microfluidic device for glycopeptide analysis includes an enrichment column capable of binding carbohydrates; a trapping column capable of binding peptides, wherein the trapping column is configured to be connected downstream of the enrichment column; a separation column, wherein the separation column is configured to be connected downstream of the trapping column; and a plurality of ports configured to work with a switching device to form a plurality of flow paths, wherein one of the plurality of flow paths allows the trapping column to be in fluid communication with the separation column. A method for glycopeptide analysis using a microfluidic device comprising a trapping column and a separation column, the method includes applying a sample of peptides to the microfluidic device; trapping the peptides on the trapping column; eluting the peptides from the trapping column into the separation column; and separating the peptides on the separation column. | 2014-01-30 |
20140030733 | Method for Diagnosing Neurodegenerative Diseases - The present invention relates to a method for diagnosing, in vitro, a neurodegenerative disease in an individual, in which:—the level of the double-stranded RNA-dependent protein kinase (PKR) in a sample of cerebrospinal fluid from the individual is determined,—it is deduced therefrom whether the individual is suffering from a neurodegenerative disease. | 2014-01-30 |
20140030734 | Biomarkers for Diseases of the Central Nervous System - The present invention describes a method of diagnosis and/or progression of a disease based on the detection and quantification of the expression levels of biomarkers preferably selected from the group comprising gelsolin isoforms (SEQ. ID 1 and 2) and Vitamin D binding protein isoforms (SEQ. ID 3 and 4). Said method is applied to central nervous system diseases, preferably to Multiple Sclerosis. | 2014-01-30 |
20140030735 | BIOMARKERS OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE - The methods described herein are based on the discovery that the plasma level of a panel of specific proteins differs between two subject populations: 1) subjects at risk for chronic obstructive pulmonary disease (“COPD”) but not manifesting clinical symptoms of COPD; and 2) subjects having very severe COPD. The difference in plasma levels is statistically significant for each protein. The identification of these proteins thus facilitates susceptibility detection, early disease detection, disease severity assessment, disease progression monitoring, and therapy efficacy monitoring. | 2014-01-30 |
20140030736 | PEPTIDE APTAMERS AGAINST TENASCIN C - The present invention relates to an anti-tenascin C peptide aptamer having a specific amino acid sequence and a diagnosis kit comprising it. The anti-tenascin C peptide aptamer of the instant invention shows a predominant clearance rate due to its small molecular weight as well as specific binding affinity to tenascin C, having excellent advantages in in vivo or ex vivo tumor imaging. | 2014-01-30 |
20140030737 | IMAGE ANALYSIS AND MEASUREMENT OF BIOLOGICAL SAMPLES - Methods, devices, systems, and apparatuses are provided for the image analysis of measurement of biological samples. | 2014-01-30 |
20140030738 | SOLUBLE HUMAN M-CSF RECEPTOR AND USES THEREOF - Soluble human M-CSF receptor is provided, along with pharmaceutical compositions containing such receptor, kits containing a pharmaceutical composition, and methods of diagnosing and treating diseases and disorders associated with M-CSF such as bone loss in a subject afflicted with an osteolytic disease. | 2014-01-30 |
20140030739 | MONOCLONAL ANTIBODIES AGAINST GMF-B ANTIGENS, AND USES THEREFOR - The disclosure relates to anti-glial maturation factor beta (“GMF-B”) monoclonal antibodies (mAbs) and fragments thereof, as well as hybridoma lines that secrete antibodies or fragments. Therapeutic and diagnostic uses of such antibodies, including treatment and detection of cancer and dementia, and methods and kits for detecting cells or samples expressing GMF-B, including soluble GMF-B, are also encompassed. | 2014-01-30 |
20140030740 | Rapid Bioluminescence Detection System - An assay is provided for detecting the activity of a reporter kinase comprising (i) adding said reporter kinase to an assay mixture wherein said reporter kinase is contacted with bioluminescent reagent no more than 5 minutes after being contacted with ADP, and wherein, prior to contacting the reporter kinase with ADP, the assay mixture is substantially free from kinase other than reporter kinase; and (ii) detecting light output from the assay mixture. Methods for detecting the presence of an analyte in a sample and methods for validating a treatment process using the above assay are also provided. Further provided are devices for conducting these assays and methods. | 2014-01-30 |
20140030741 | In Vitro Method for Determining Presence of Type II Pyrethroids - A method of determining the presence of type II pyrethroid compounds in a sample. | 2014-01-30 |
20140030742 | ANTIBODY REACTING WITH NATIVE COCHLIN-TOMOPROTEIN (CTP) AND METHOD FOR MEASURING CTP USING SAME - An immunological measurement is performed using anti-CTP antibody characterized by recognizing an antigenic determinant included in the polypeptide represented by amino acid numbers 118-132 of SEQ ID NO: 1, and reacting with native Cochlin-tomoprotein (CTP). | 2014-01-30 |
20140030743 | MARKERS FOR PREECLAMPSIA - This document provides methods and materials related to determining whether or not a pregnant mammal (e.g., a pregnant human) has preeclampsia. For example, methods and materials related to the use of urinary podocytes to determine whether or not a pregnant human has preeclampsia are provided. | 2014-01-30 |
20140030744 | Means and Methods for Diagnosing Gastric Bypass and Conditions Related Thereto - The present invention relates to the field of diagnostic measures. Specifically, it contemplates a method for assessing whether gastric bypass therapy was successful in a subject, a method of predicting whether gastric bypass therapy will be beneficial for a subject in need thereof, and a method of diagnosing whether a supportive therapy accompanying gastric bypass has beneficial effects on a subject in need thereof. Further provided are diagnostic methods for diabetes and body lean mass. Furthermore, the invention relates to a method for identifying a treatment against diabetes and/or obesity. | 2014-01-30 |
20140030745 | ASSAY KIT AND ANALYSIS METHOD - An assay kit for reacting with an analyte includes a plurality of reaction vessels and a plurality of micro beads. Each of the reaction vessels contains a filter membrane with a plurality of pores, and the diameter of each micro bead is greater than that of each pore. Each of the micro beads is directly or indirectly bound to the analyte, the competitor of the analyte or the identifying molecule. Additionally, an analysis method applied with the assay kit is also disclosed. | 2014-01-30 |
20140030746 | KINASE SUBSTRATE SENSOR - The disclosure relates to a cytoplasmic protein complex comprising: (a) a first recombinant fusion protein comprising a kinase, fused to a first interaction polypeptide; and (b) a second recombinant fusion protein comprising a domain comprising a reporter phosphorylation site, whereby the domain is fused to a second interaction polypeptide. The disclosure relates further to a method to detect compound-compound-interaction using the cytoplasmic protein complex, and to cells comprising such cytoplasmic protein complex. | 2014-01-30 |
20140030747 | NANOCHANNEL-BASED SENSOR SYSTEM FOR USE IN DETECTING CHEMICAL OR BIOLOGICAL SPECIES - A sensor system for detecting a chemical or biological species includes a sensing element and a bias and measurement circuit. The sensing element includes nanochannels having an outer surface functionalized for interaction with the species to create a surface potential, and each having a sufficiently small cross section to exhibit a shift of differential conductance into a negative bias operating region by a shift amount dependent on the surface potential. The bias and measurement circuit applies a bias voltage across two ends of the nanochannels sufficiently negative to achieve a desired dependence of the differential conductance on the surface potential. The dependence has a steeply sloped region of high amplification substantially greater than a reference amplification at a zero-bias condition, thus achieving relatively high signal-to-noise ratio. The bias and measurement circuit converts the measured differential conductance into a signal indicative of presence or activity of the species. | 2014-01-30 |
20140030748 | METHOD AND SYSTEM TO MANAGE DIABETES USING MULTIPLE RISK INDICATORS FOR A PERSON WITH DIABETES - Described are methods and systems to annunciate to the patient of the components involved in each of the daily risk range based on the glucose measurements to assist the patient in identification of whether it is hypoglycemia or hyperglycemia are driving the daily risk range of the measured glucose values. | 2014-01-30 |
20140030749 | MODIFIED GLUCOSE DEHYDROGENASE - The present invention relates to glucose dehydrogenase [NAD(P) | 2014-01-30 |
20140030750 | High Throughput In Vitro Translation (Cell-Lysate Based) Assay for Detecting Quorum Sensing Signals - A cell-lysate extract based assay reagent for detecting quorum sensing signals is generally provided, along with methods of making and using the same. The assay reagent generally includes a cell-lysate extract formed from a biosensor bacterium (e.g., | 2014-01-30 |
20140030751 | Devices and Methods for the Fast Photochemical Oxidation of Proteins - Embodiments of the present disclosure provide for devices and methods that can be used in a process for the fast photochemical oxidation of proteins, and the like. | 2014-01-30 |
20140030752 | MODULAR PLATFORM FOR MULTI-TISSUE INTEGRATED CELL CULTURE - The systems and methods disclosed herein are generally related to a cell culture system. More particularly, the systems and methods enable the culturing and interconnecting of a plurality of tissue types in a biomimetic environment. By culturing organ specific tissue types within a biomimetic environment and interconnecting each of the organ systems in a physiologically meaningful way, experiments can be conducted on in vitro cells that substantially mimic the responses of in vivo cell populations. In some implementations, the system is used to monitor how organ systems respond to agents such as toxins or medications. The system enables the precise and controlled delivery of these agents, which, in some implementations, enables the biomimetic dosing of drugs in humans to be mimicked. | 2014-01-30 |
20140030753 | MRI-COMPATIBLE BIOREACTORS AND METHODS OF USING - This disclosure describes a MRI-compatible bioreactor that allows a biological sample to be imaged in culture without disrupting or compromising the culture. | 2014-01-30 |
20140030754 | BLOOD COLLECTION DEVICE CONTAINING LYSOPHOSPHOLIPASE INHIBITOR - Disclosed are collection devices for collecting and stabilizing whole blood or a component thereof, which include a first end and a second end and at least one interior wall defining a reservoir, wherein the reservoir contains a stabilization agent that includes a lysophospholipase (LysoPLA) inhibitor. Also disclosed are methods for making and using the devices. | 2014-01-30 |
20140030755 | MICROPLATE FOR CORRELATIVE MICROSCOPY - The invention relates to a novel multiwell array comprising a plurality of wells, the majority of which have a unique cross-sectional shape whereby each well within the array can be identified and so tracked whilst the contents of the array are investigated. The invention also concerns a computer and a programmable data storage device for use with the array and an imaging apparatus including any one or more of the afore features or aspects. | 2014-01-30 |
20140030756 | IDENTIFYING AND ENUMERATING EARLY GRANULATED CELLS (EGCS) - Methods and systems for automatically identifying and enumerating early granulated cells (EGC) in blood samples are disclosed. In one embodiment a method for identifying EGC in a blood sample includes analyzing white blood cells of the blood sample using a low angle light scatter (LALS) parameter, separating the EGCs from the other white blood cells using the LALS parameter, and enumerating the separated EGCs. | 2014-01-30 |
20140030757 | GUIDED SLICING SYSTEM FOR OBTAINING HISTOLOGICAL SAMPLES AND METHODS THEREOF - Systems and methods for providing guided slicing of histological samples. The samples are acquired, spacially labeled with a fiducial marker, and imaged with a scanning system. The images are analyzed, either manually, semi-automatically or automatically, and likely locations of pathologies are identified. A slicing program is then generated and the sample sliced according to the slicing program, thereby ensuring that likely locations of pathologies are analyzed. | 2014-01-30 |
20140030758 | RECOMBINANT POLYPEPTIDE PRODUCTION METHOD - Provided is a method capable of producing a protein at a high level using a cultured animal cell, comprising culturing a cell that expresses APES (Antibody Production Enhancing Sequence) and into which a DNA encoding a desired polypeptide has been introduced, thereby producing the desired polypeptide. APES contains a nucleotide sequence related to NfkBia and has a function of decreasing the intracellular expression of NfkBia. | 2014-01-30 |
20140030759 | Methods Of Intracellular Conversion Of Single-Chain Proteins Into Their Di-Chain Form - The present specification discloses expression constructs comprising single-chain proteins comprising a di-chain loop region comprising an exogenous protease cleavage site and a protease that can cleave the exogenous protease cleavage site located within the di-chain loop, cell compositions comprising such expression construct, and intracellular methods of converting the single-chain protein into its di-chain form. | 2014-01-30 |
20140030760 | PAVEC - This application provides, in part, pAVEC plasmids and methods of use of such plasmids for the production of polypeptides such as immunoglobulins as well as the generation of recombined versions of such plasmids which contain polynucleotides encoding such polypeptides. | 2014-01-30 |
20140030761 | METHOD OF CLONING NUCLEIC ACID - The present disclosure provides methods for producing expression constructs comprising linking a plurality of unlinked nucleic acids, including nucleic acid encoding a marker protein. | 2014-01-30 |
20140030762 | BIOREACTOR FOR CELL CULTURE ON A THREE-DIMENSIONAL SUBSTRATE - The invention relates to a bioreactor ( | 2014-01-30 |
20140030763 | Processing Biomass Containing Materials - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation. | 2014-01-30 |
20140030764 | METHODS AND DEVICES FOR ELECTROMAGNETIC AMPLIFICATION OF NUCLEIC ACIDS - Methods and devices for amplifying nucleic acids generally involve exposing the nucleic acid to an electromagnetic field, for example a mini-current magnetic field, while performing the steps of PCR. The PCR methods and devices provide numerous advantages over conventional PCR techniques and systems such as reduced reaction times, no heating requirements, and reduced amounts of reagents (e.g., optional use polymerases and primers). Additionally, the PCR methods and devices require significantly shorter reaction times (e.g., less than one hour) compared to conventional PCR techniques and systems (minimum 2 hours). Finally, as shown in the Examples, the PCR methods and devices amplify significantly more DNA compared to conventional PCR techniques and systems. Accordingly, the PCR methods and devices provide a more efficient and cost-effective way to perform PCR when compared to conventional PCR techniques and systems. | 2014-01-30 |
20140030765 | MODIFIED DNA POLYMERASES FOR IMPROVED AMPLIFICATION - The present invention provides improved DNA polymerases that may be better suited for applications in recombinant DNA technologies, in particular technologies involving plant-derived samples. Among other things, the present invention provides modified DNA polymerases derived from directed evolution experiments designed to select mutations that confer advantageous phenotypes under conditions used in industrial or research applications. | 2014-01-30 |
20140030766 | Method And Device - The present invention provides a method for the assembly of a polynucleic acid sequence from a plurality of nucleic acid sequences in which the polynucleic acid sequence is of a formula N | 2014-01-30 |
20140030767 | Alpha-Amylase Mutants with Altered Properties - The present invention relates to variants (mutants) of parent Termamyl-like alpha-amylases, which variant has alpha-amylase activity and exhibits altered stability, in particular at high temperatures and/or at low pH relative, and/or low Ca | 2014-01-30 |
20140030768 | Processing Biomass Containing Materials - Biomass (e.g., plant biomass, animal biomass, and municipal waste biomass) is processed to produce useful products, such as fuels. For example, systems can use feedstock materials, such as cellulosic and/or lignocellulosic materials and/or starchy materials, to produce ethanol and/or butanol, e.g., by fermentation. | 2014-01-30 |
20140030769 | FREE ENZYME AND CELLULOSOME PREPARATIONS FOR CELLULOSE HYDROLYSIS - Disclosed herein are combinations of free fungal enzymes and cellulosomes useful for the hydrolysis of cellulose and the conversion of biomass. Methods of degrading cellulose and biomass using the combinations are also disclosed. | 2014-01-30 |
20140030770 | Polypeptides Having Cellobiohydrolase Activity and Polynucleotides Encoding Same - The present invention relates to isolated polypeptides having cellobiohydrolase activity and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2014-01-30 |
20140030771 | COMPOSITIONS AND METHODS FOR INCREASING OIL PRODUCTION AND SECRETION - The present invention provides compositions and methods related to the production of fatty acids, such as triglycerides with genetically engineered cells, such as algae. | 2014-01-30 |
20140030772 | HIGH EICOSAPENTAENOIC ACID PRODUCING STRAINS OF YARROWIA LIPOLYTICA - Engineered strains of the oleaginous yeast | 2014-01-30 |
20140030773 | MODIFIED MICROORGANISM FOR HIGH EFFICIENT PRODUCTION OF 1,4-BUTANEDIOL - A modified microorganism for producing a high yield of 1,4-butanediol, and a method of producing 1,4-butanediol using the modified microorganism, wherein the modified microorganism is altered so as to delete or disrupt expression of at least one of transcription regulatory factor NCg12886, GCN5-related N-acetyltransferase NCg12090, hypothetical protein NCg10224 or sugar phosphate isomerase/epimerase NCg12956. | 2014-01-30 |
20140030774 | MUTANT OF PROPIONYL-COA TRANSFERASE FROM CLOSTRIDIUM PROPIONICUM AND PREPARING METHOD FOR PLA OR PLA COPOLYMER USING THE SAME - Provided is a mutant of propionyl-CoA transferase from | 2014-01-30 |
20140030775 | RECOMBINANT MICROORGANISM HAVING ABILITY TO PRODUCE [LACTATE-CO-GLYCOLATE] COPOLYMER FROM GLUCOSE, AND METHOD FOR PREPARING [LACTATE-CO-GLYCOLATE] COPOLYMER USING SAME - There is provided a recombinant microorganism having producibility of poly(lactate-co-glycolate) from glucose, and more particularly, a recombinant microorganism having producibility of poly(lactate-co-glycolate) without adding an exogenous glycolate precursor, and a method of preparing [poly(preparing lactate-co-glycolate)] using the same. According to the present invention, the poly(lactate-co-glycolate) in which the concentration of the glycolate fraction is high may be prepared at a high concentration without supplying exogenous glyoxylate. Therefore, the present invention may be effectively used for treatment. | 2014-01-30 |
20140030776 | IDENTIFICATION AND USE OF BACTERIAL [2Fe-2S] DIHYDROXY-ACID DEHYDRATASES - A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to α-ketoisovalerate or 2,3-dihydroxymethylvalerate to α-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity. | 2014-01-30 |
20140030777 | APPARATUS AND METHOD FOR SEPARATING AND REFINING PRODUCT MANUFACTURED BY MICROBIAL FERMENTATION BY USING ADSORBENT - The present invention relates to an apparatus and a method for fermenting, separating, and refining a product, which is produced by cultivating a microorganism. The apparatus and the method for fermenting, separating, and refining, of the present invention, can separate and refine the product that is produced by microbial fermentation in a simple, continuous manner and with high efficiency. | 2014-01-30 |
20140030778 | Bacterial Strain and Process for the Fermentative Production of Organic Acids - The present invention relates to a novel bacterial strain designated DD1, which has the ability to produce organic acids, in particular succinic acid (SA), which was originally isolated from bovine rumen, and is capable of utilizing glycerol as a carbon source; and variant strains derived there from retaining said capability; as well as to methods of producing organic acids, in particular succinic acid by making use of said microorganism. | 2014-01-30 |
20140030779 | MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS - The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms. | 2014-01-30 |
20140030780 | METHODS AND ORGANISMS FOR THE GROWTH-COUPLED PRODUCTION OF 1,4-BUTANEDIOL - The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby the one or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO. | 2014-01-30 |
20140030781 | MUTANT MICROORGANISM HAVING IMPROVED 1,4-BDO PRODUCTIVITY AND METHOD OF PREPARING 1,4-BDO USING THE MUTANT MICROORGANISM - A recombinantly modified | 2014-01-30 |
20140030782 | HOST CELLS AND METHODS FOR PRODUCTION OF ISOBUTANOL - Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity. | 2014-01-30 |
20140030783 | HOST CELLS AND METHODS FOR PRODUCTION OF ISOBUTANOL - Provided herein are recombinant yeast host cells and methods for their use for production of isobutanol. Yeast host cells provided comprise an isobutanol biosynthetic pathway and at least one of reduced or eliminated aldehyde dehydrogenase activity, reduced or eliminated acetolactate reductase activity; or a heterologous polynucleotide encoding a polypeptide having ketol-acid reductoisomerase activity. | 2014-01-30 |