03rd week of 2016 patent applcation highlights part 24 |
Patent application number | Title | Published |
20160017268 | DEVICE AND METHODS COMPRISING MICROELECTRODE ARRAYS FOR ELECTROCONDUCTIVE CELLS - The present invention relates to the devices and method comprising microelectrode arrays for the differentiation, maturation and functional analysis of electroconductive cells, including muscle cells (including, but not limited to, cardiomyocytes, skeletal muscle myocytes and smooth muscle myocytes) and neuronal cells. The microelectrode present on the arrays can be used to stimulate and record from cells cultured on the substrate. In some embodiments, the substrate has a substantially smooth surface, and in other embodiments the substrate is nanotextured, including an array of substantially parallel grooves and ridges of nanometer-micrometer widths. | 2016-01-21 |
20160017269 | BIOREACTOR AND SENSING SYSTEM THEREFOR - The present invention relates to a measurement probe system ( | 2016-01-21 |
20160017270 | Apparatus, Method, and System for Cultured Sample Development Monitoring - The present invention relates to the field of testing and evaluation of biological samples and provides apparatus for cultured samples comprising at least one independently accessible module adapted for incubating at least one of a plurality of samples wherein the at least one module is operatively associated with a light source and a movable optical inspection means adapted for motion about a viewing axis through the module to enable a sweeping of viewing area. The invention also provides a method of assessing cultured samples for viability comprising the steps of: disposing biological samples in a substantially elliptical arrangement within a culturing chamber of an independently accessible module; imaging individual samples of the substantially elliptical arrangement with optical inspection means driven within an X-Y plane that is normal to a viewing axis through the module to obtain time lapse measurement of development of individual samples. | 2016-01-21 |
20160017271 | CELL CULTURING DEVICE, CULTURING VESSEL, AND HOLDING VESSEL - In culture vessels for producing biological samples, it is possible to prevent decrease in a temperature at the time of taking out the culture vessels from an automatic culture apparatus and during delivery of the culture vessels which have been taken out from the automatic culture apparatus. This invention includes a culture vessel | 2016-01-21 |
20160017272 | Devices and Methods for Purification, Detection and Use of Biological Cells - This invention relates to devices and methods for purifying, detecting and using biological cells. A variety of cell types including viable tumor, stem, immune and sperm cells can be purified from a complex biological sample using a column, including a pipette tip column. Methods of the invention can aid research, diagnosis and treatment of cancer. Purified viable cells can be detected on the column or eluted from the column and detected. Cells on a column can be used as a stationary phase for liquid chromatography. Cells may be removed, recovered and analyzed. | 2016-01-21 |
20160017274 | DEVICE AND METHOD FOR TREATING A FILTRATION MEDIUM | 2016-01-21 |
20160017275 | USE OF ATTENUATED PARASITE STRAINS FOR THE PREVENTION AND/OR TREATMENT OF EYE WOUNDS ASSOCIATED WITH AN INFECTION BY TOXOPLASMA GONDII - Strains of | 2016-01-21 |
20160017276 | NOVEL BACTERIA AND METHODS OF USE THEREOF - The invention provides a novel bacterium with improved properties, including improved ethanol production, ethanol productivity, CO uptake, specific growth rate, ethanol to acetate ratio, and alcohol tolerance. The bacterium may be derived from | 2016-01-21 |
20160017277 | CELL CULTURE MEDIA COMPOSITION AND METHODS OF PRODUCING THEREOF - A serum free cell culture media, wherein the media is adapted to be conditioned by culturing a first set of eukaryotic cells in the media, wherein the first set of eukaryotic cells use an expression vector to excrete levels of desired complex proteins into the media; wherein said desired complex proteins include human Growth Hormone (hGH), Growth Hormone-like growth factors, insulin-like growth factors, insulin, modified insulins, cytokines, mitogenic proteases and mixtures thereof; and wherein the media is adapted to grow a set of eukaryotic cells. | 2016-01-21 |
20160017278 | PROTEIN NANOFIBERS FROM SELF-ASSEMBLING PENTAMERS - A protein nanofiber comprised of self-assembling pentamers, and a method for producing the protein nanofiber, in which the protein upon which the nanofiber is based is a specific form of COMP. The proteins self-assemble via electrostatic interactions to form fibers that extend longitudinally. | 2016-01-21 |
20160017279 | SURFACE IMMOBILIZATION OF VARIOUS FUNCTIONAL BIOMOLECULES USING MUSSEL ADHESIVE PROTEIN - The present invention relates to technology of immobilizing or coating various functional bioactive substances on various surfaces without physical chemical treatment using mussel adhesive protein. More specifically, the present invention relates to a functional scaffold for tissue engineering comprising artificial extracellular matrix, manufactured by coating various functional bioactive substances on the surface of nanofiber and metal scaffold using mussel adhesive protein, and a method of manufacturing the same. | 2016-01-21 |
20160017280 | Perfusion Culturing Methods and Uses Thereof - Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. Also provided are multi-well cell culture plates, e.g., for use in perfusion culturing methods. | 2016-01-21 |
20160017281 | CELL EXPRESSION SYSTEM - An expression system for expressing a protein comprising: a eukaryotic host cell carrying a dihydrofolate reductase (DHFR) deficiency; and an expression vector, the expression vector encoding the human growth hormone gene; a expression vector, the expression vector comprising: a eukaryotic selectable marker including a minimal SV 40 early promoter driving expression of a sequence encoding dihydrofolate reductase for complementing the DHFR deficiency in the host cell; a prokaryotic selectable marker conveying Ampicillin resistance to a prokaryotic host cell; a prokaryotic Origin of Replication; a plurality of multiple cloning sites (MCS); and at least one protein expression module comprising: a Simian Vacuolating Virus 40 (SV40) early promoter, inclusive of its 72 bp enhancer repeats; and a rabbit β-globin intron sequence being separable from a SV40 p A sequence by a first multiple cloning site, for receiving a coding sequence and expressing a desired protein therefrom. | 2016-01-21 |
20160017282 | ENHANCED PLACENTAL STEM CELLS AND USES THEREOF - Provided herein are placental stem cells that exhibit increased survival (“enhanced placental stem cells”), compositions comprising such placental stem cells, and methods of using such placental stem cells and compositions. | 2016-01-21 |
20160017283 | SYSTEMS AND METHODS FOR CULTURING EPITHELIAL CELLS - The present invention features assays for co-culturing primary cells while maintaining key biological activities specific to the primary cells. The invention is based, at least in part, on the discovery that compositions and methods for primary cells in a high-throughput co-culture platform, image analysis for distinguishing cells in co-cultures and assays that are suitable for screening of agents in epithelial cells, such as hepatocytes. | 2016-01-21 |
20160017284 | CYPRINID HERPESVIRUS II-SENSITIVE BRAIN TISSUE CELL LINE OF CARASSIUS AURATUS GIBELIO AND ESTABLISHING METHOD AND USE THEREOF - The present invention discloses a cyprinid herpesvirus II-sensitive brain tissue cell line of | 2016-01-21 |
20160017285 | NEURAL PROGENITOR CELL DIFFERENTIATION - Differentiation and stability of neural stem cells can be enhanced by in vitro or in vivo culturing with one or more extracellular matrix (ECM) compositions, such as collagen I, IV, laminin and/or a heparan sulfate proteoglycan. In one aspect of the invention, adult mammalian enteric neuronal progenitor cells can be induced to differentiate on various substrates derived from components or combinations of neural ECM compositions. Collagen I and IV supported neuronal differentiation and extensive glial differentiation individually and in combination. Addition of laminin or heparan sulfate to collagen substrates unexpectedly improved neuronal differentiation, increasing neuron number, branching of neuronal processes, and initiation of neuronal network formation. In another aspect, neuronal subtype differentiation was affected by varying ECM compositions in hydrogels overlaid on intestinal smooth muscle sheets. The matrix compositions of the present invention can be used to tissue engineer transplantable innervated GI smooth muscle constructs to remedy aganglionic disorders. | 2016-01-21 |
20160017286 | IKAROS INHIBITION TO AUGMENT ADOPTIVE T CELL TRANSFER - The present invention provides compositions and methods for inhibiting Ikaros in a cell in order to enhance the cytolytic activity of the cell. In one embodiment, the cells may be used in adoptive T cell transfer. For example, in some embodiments, the cell is modified to express a chimeric antigen receptor (CAR). Inhibition of Ikaros in T cells used in adoptive T cell transfer increases cytolytic activity of the T cells and thus may be used in the treatment of a variety of conditions, including cancer, infection, and immune disorders. | 2016-01-21 |
20160017287 | SYSTEM FOR EXTRACTION OF CELLS FROM A SAMPLE OF TISSUE - A system for extraction of cells from a sample of tissue comprises a main device ( | 2016-01-21 |
20160017288 | METHOD FOR INDUCING THE FORMATION OF ISLET STRUCTURES AND IMPROVING BETA CELL FUNCTION - Insulin producing β cells are found in three dimensional (3D) structures, the Islet of Langerhans. The 3D structure is required for normal β cell function and survival. β cell pseudoislets (PIs) are useful for study of β cell physiology. Co-culturing of primary human islets and β cell lines together with islet-derived epithelial cells can improve β cell function and survival and maintain the cells' 3D structure, resulting a rapid and spontaneous formation of free-floating PIs. β cells in PIs were similar in size to native islets and showed increased percentage of pro-insulin-positive cells, increased insulin gene expression in response to glucose stimulation, improved glucose-stimulated insulin secretion, and reduced β cell death. Key ECM proteins, absent in monolayer β cells, are deposited by iECs in and round the PIs. iEC induced PIs are a useful tool for examining β-cell/iEC interactions and studying β-cell function in a native 3D configuration. | 2016-01-21 |
20160017289 | TGIF2-INDUCED REPROGRAMMING OF HEPATIC CELLS TO PANCREATIC PROGENITOR CELLS AND MEDICAL USES THEREOF - Disclosed are genetically modified human pancreatic progenitor cells, comprising an exogenous nucleic acid molecule encoding TGIF2, for use as a medicament in the treatment of a subject with diabetes, in addition to methods for the production of said cells. Also disclosed is an expression vector encoding TGIF2 for use as a medicament in the treatment of a subject with diabetes. | 2016-01-21 |
20160017290 | METHODS OF INCREASING INSULIN CONTENT IN CELLS - A method of ex-vivo increasing insulin content in progenitor cells which express Zinc Finger E-Box Binding Homeobox 1 (ZEB-1) is disclosed. The method comprises contacting the progenitor cells with an inhibitory agent directed against a polypeptide, wherein the RNA transcript encoding said polypeptide is targeted by miRNA-200c, said polypeptide being selected from the group consisting of ZEB-1, SOX-2 and SOX-6. | 2016-01-21 |
20160017291 | MICROCARRIER PERFUSION CULTURING METHODS AND USES THEREOF - Provided herein are methods of culturing a mammalian cell and various methods that utilize these culturing methods. | 2016-01-21 |
20160017292 | SELECTIVE TARGETING OF CANCER STEM CELLS - Aspects of the invention relate to methods and related compositions for preferentially targeting cancer stem cells. In some embodiments, the methods utilize PKC-α/FRA1 pathway inhibitors to target carcinoma cells. Also provided are methods for identifying a candidate compound for selectively inhibiting growth of cancer stem cell, and methods for obtaining cells that have undergone an epithelial to mesenchymal transition. | 2016-01-21 |
20160017293 | INDIVIDUALIZED HIGH PURITY HEPATOCELLULAR CARCINOMA STEM CELLS, METHODS AND USE OF THE SAME - The disclosure provides cancer stem cells, for use in stimulating immune response against a cancer, such as hepatocellular carcinoma (HCC). Methods for preparing and purifying the cancer stem cells are provided. | 2016-01-21 |
20160017294 | TUMOR-SELECTIVE E1A AND E1B MUTANTS - Modified E1a regulatory sequences are provided, wherein at least one Pea3 binding site, or a functional portion thereof, is deleted. Also provided are modified E1a sequences that selectively express particular isoforms. Also provided is an E1b-19K clone insertion site. These modified sequences can be used individually, or in combination with one another, to provide tumor-selective expression of proteins. | 2016-01-21 |
20160017295 | ADENO-ASSOCIATED VIRUS VARIANTS AND METHODS OF USE THEREOF - The present disclosure provides infectious recombinant adeno-associated virus (rAAV) virions that comprise a variant capsid protein and a heterologous nucleic acid. The present disclosure further provides the variant adeno-associated virus (AAV) capsid proteins (and/or a nucleic acid encoding the variant AAV capsid proteins), which confer to an infectious rAAV virion an increased resistance to human AAV neutralizing antibodies. The present disclosure further provides host cells comprising an infectious rAAV virion and/or a nucleic acid encoding a subject variant AAV capsid protein. The present disclosure further provides methods of delivering a heterologous nucleic acid to a target cell where the target cell is contacted with a subject infectious rAAV virion. The present disclosure further provides methods of delivering a gene product to an individual, the methods generally involving administering an effective amount of a subject rAAV virion to an individual in need thereof. | 2016-01-21 |
20160017296 | Dehydrogenase Variants and Polynucleotides Encoding Same - The present invention relates to 3-hydroxypropionate dehydrogenase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants. | 2016-01-21 |
20160017297 | RECOMBINANT MANGANESE OXIDASE - Disclosed herein is a recombinant | 2016-01-21 |
20160017298 | Mutants of Cre Recombinase - The present invention relates to mutants of Cre recombinase. | 2016-01-21 |
20160017299 | OBLIGATE HETERODIMER VARIANTS OF FOKI CLEAVAGE DOMAIN - Disclosed are methods of making and using engineered Fold cleavage domain variants. Also disclosed are methods, compositions and fusion proteins containing obligate heterodimers of engineered Fold cleavage domain variants and DNA binding domains, such as zinc finger protein (ZFP) domains and transcription activator-like effector (TALE) domains. | 2016-01-21 |
20160017300 | METHOD FOR USING HEAT-RESISTANT MISMATCH ENDONUCLEASE - Provided are a mismatch-specific cleavage reaction using a novel heat-resistant mismatch nuclease, a method for removing errors in a nucleic acid amplification reaction using the mismatch nuclease, a method for inhibiting the amplification of a nucleic acid having a specific base sequence during a nucleic acid amplification reaction, and a method for detecting a nucleic acid having a single-base polymorphic mutation using this inhibition method. | 2016-01-21 |
20160017301 | METHODS AND COMPOSITIONS FOR RNA-GUIDED TREATMENT OF HIV INFECTION - A method of inactivating a proviral DNA integrated into the genome of a host cell latently infected with a retrovirus by treating the host cell with a composition comprising a Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)-associated endonuclease, and two or more different guide RNAs (gRNAs), wherein each of the at least two gRNAs is complementary to a different target nucleic acid sequence in a long terminal repeat (LTR) in the proviral DNA, and inactivating the proviral DNA. A composition for use in inactivating a proviral DNA integrated into the genome of a host cell latently infected with a retrovirus including isolated nucleic acid sequences comprising a CRISPR-associated endonuclease and a guide RNA, wherein the guide RNA is complementary to a target sequence in a human immunodeficiency virus. | 2016-01-21 |
20160017302 | HEPARANASE EXPRESSION IN HUMAN T LYMPHOCYTES - Embodiments of the present disclosure concern improvements to cell therapy for cancer. In certain embodiments, an ex vivo expanded T cell lacks endogenous heparanase expression, and amelioration of this effect allows an improvement for cancer cell therapy, including of solid tumors. In specific embodiments, ex vivo expanded T cells comprise recombinant heparanase expression. | 2016-01-21 |
20160017303 | ALPHA-AMYLASE COMBINATORIAL VARIANTS - Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing. | 2016-01-21 |
20160017304 | ALPHA-AMYLASE COMBINATORIAL VARIANTS - Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing. | 2016-01-21 |
20160017305 | ALPHA-AMYLASE COMBINATORIAL VARIANTS - Disclosed are compositions and methods relating to variant alpha-amylases. The variant alpha-amylases are useful, for example, for starch liquefaction and saccharification, for cleaning starchy stains in laundry, dishwashing, and other applications, for textile processing (e.g., desizing), in animal feed for improving digestibility, and for baking and brewing. | 2016-01-21 |
20160017306 | Methods for Using Polypeptides Having Cellobiohydrolase Activity - The present invention relates to isolated polypeptides having cellobiohydrolase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides. | 2016-01-21 |
20160017307 | POLYPEPTIDES HAVING ENDOLYSIN ACTIVITY AND USES THEREOF - The present invention relates to novel polypeptides derived from endolysins from a bacteriophage of | 2016-01-21 |
20160017308 | USE OF N-ACETYLNEURAMINIC ACID ALDOLASE IN CATALYTIC SYNTHESIS OF N-ACETYLNEURAMINIC ACID - It discloses a use of N-acetylneuraminic acid aldolase with an amino acid sequence as shown in SEQ ID NO: 2 in catalytic synthesis of N-acetylneuraminic acid. The preparation of N-acetylneuraminic acid is to use the N-acetylneuraminic acid aldolase with the amino acid sequence as shown in SEQ ID NO: 2 as a catalyst, and N-acetylmannosamine and pyruvic acid as substrates. | 2016-01-21 |
20160017309 | Human Cystathionine Beta-Synthase Variants and Methods of Production Thereof - Human cystathionine β-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine β-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologues are described. In addition, a method to express and purify recombinant human cystathionine β-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described. | 2016-01-21 |
20160017310 | XYLOSE ISOMERASES AND THEIR USES - This disclosure relates to novel xylose isomerases and their uses, particularly in fermentation processes that employ xylose-containing media. | 2016-01-21 |
20160017311 | INNOVATIVE DISCOVERY OF THERAPEUTIC, DIAGNOSTIC, AND ANTIBODY COMPOSITIONS RELATED TO PROTEIN FRAGMENTS OF GLYCYL-TRNA SYNTHETASES - Provided are compositions comprising newly identified protein fragments of aminoacyl-tRNA synthetases, polynucleotides that encode them and complements thereof, related agents, and methods of use thereof in diagnostic, drug discovery, research, and therapeutic applications. | 2016-01-21 |
20160017312 | METHOD FOR MODULATING A BACTERIAL INVASION SWITCH - The subject matter disclosed herein pertains to the modulation of a bacterial invasion switch and the subsequent use of the bacterium to vaccinate an organism. In one embodiment, the bacterial invasion switch is modulated by changing the proteolysis of ExoR protein. In another embodiment, a mutated bacterium produces a mutant ExoR protein that resists proteolysis. | 2016-01-21 |
20160017313 | ANALYSIS OF MRNA HETEROGENEITY AND STABILITY - Reversed phase-High Performance (High Pressure) Liquid Chromatography (RP-HPLC) and Size Exclusion Chromatography (SEC) methods have been developed for monitoring structural and size heterogeneity as well as stability of large RNA transcripts, including lengths of up to at least 10,000 nucleotides. The methods are designed for significantly larger mRNAs that could be monitored in the past, including lengths of up to at least 10,000 nucleotides, and including chemically modified RNA transcripts. SEC techniques are also used in the preparative purification of large RNA transcripts to remove impurities, including hybridized nucleic acid impurities and multimeric RNA species. All of these techniques are also beneficial in that they can be used for large scale manufacturing of therapeutics. | 2016-01-21 |
20160017314 | METHOD FOR ISOLATING NUCLEIC ACIDS FROM FORMALIN-FIXED PARAFFIN EMBEDDED TISSUE SAMPLES - Methods are disclosed for isolating nucleic acids from formalin-fixed paraffin embedded (FFPE) tissue samples. Each of tissue samples contains paraffin and a target biological tissue or material, and the method includes the steps of: adding a first reagent and a second reagent to the FFPE tissue sample, the first reagent dissolving the paraffin material and the second reagent lysing the biological tissue; mixing the first reagent, the second reagent, and the FFPE tissue sample to form a first mixture; (2) heating the first mixture at 50-80° C. for 30-90 minutes; and then heating the first mixture at 80-95° C. for 30-90 minutes to fractionize the first mixture to form an aqueous phase and an oil phase; (3) collecting an aqueous solution from the aqueous phase; and (4) isolating nucleic acids from the aqueous solution. The method improves the efficiency and convenience of isolating nucleic acids from FFPE tissue samples. | 2016-01-21 |
20160017315 | METHODS FOR ONE STEP NUCLEIC ACID AMPLIFICATION OF NON-ELUTED SAMPLES - The present invention relates to methods and kits which can be used to amplify nucleic acids with the advantage of decreasing user time and possible contamination. For easy processing and amplification of nucleic acid samples, the samples are bound to a solid support and used directly, without purification, in a nucleic acid amplification reaction such as the polymerase chain reaction (PCR). | 2016-01-21 |
20160017316 | ISOLATION OF MEGABASE-SIZED DNA FROM PLANT AND ANIMAL TISSUES - Disclosed herein are methods for isolation of long DNA molecules, for example megabase-sized genomic DNA molecules, from a biological sample, for example plant and animal tissues. | 2016-01-21 |
20160017317 | Methods For Selecting Microbes From A Diverse Genetically Modified Library to Detect and Optimize the Production of Metabolites - The present invention relates to genetically modified bacteria and methods of optimizing genetically modified bacteria for the production of a metabolite. | 2016-01-21 |
20160017318 | GENE SIGNATURES TO IDENTIFY MOLECULAR SUBGROUPS IN MEDULLOBLASTOMA TUMORS - Described herein are methods for determining the subgroup of medulloblastoma in a subject in need thereof. The subgroups of medulloblastoma include Group 3, Group 4, WNT and SHH. The subjects are children diagnosed with medulloblastoma or children suspected of having medulloblastoma. | 2016-01-21 |
20160017319 | Method of screening cell clones - A method of screening cell clones expressing a high yield of a polypeptide of interest is provided. The method employs the consecutive use of fluorescence activated cell sorting followed by colony picking based selection of cell clones with high expression rates and high proliferation rates. Furthermore, the invention pertains to a method of producing a polypeptide of interest using cells obtained by the described screening method. | 2016-01-21 |
20160017320 | SEMI-RANDOM BARCODES FOR NUCLEIC ACID ANALYSIS - The present disclosure provides oligonucleotides that comprise semi-random barcode sequences. Such oligonucleotides may be incorporated into reverse transcription primers, PCR primers, or portions of sequencing adapters in preparing sequencing libraries. The resulting sequencing libraries can be used for accurate sequencing, including DNA or RNA counting and mutation detection. Methods and kits for preparing sequencing adapters and sequencing libraries are also provided. | 2016-01-21 |
20160017321 | METHOD FOR EXPRESSING, SECRETING AND TRANSFERRING FUNCTIONAL MICRORNAS/SIRNAS AND APPLICATION THEREOF - The present invention relates to a microparticle comprising a functional microRNA/siRNA and application thereof. Specifically, disclosed is a use of a functional microRNA and/or siRNA, which is used to prepare a composition applied to a mammal. After the composition is applied, the microparticle is formed in a first part in an animal, and transported to a second part, which thereby improves physiological status of the second part or heals a disease of the second part. The second part is different from the first part. The use is beneficial for optimizing a dosing manner of the functional microRNA and/or siRNA. | 2016-01-21 |
20160017322 | REDUCING INTRON RETENTION - Disclosed herein are methods, compositions, polynucleic acid polymers, assays, and kits for inducing processing of a partially processed mRNA transcript to remove a retained intron to produce a fully processed mRNA transcript that encodes a full-length functional form of a protein. Also described herein are methods and compositions for treating a disease or condition characterized by impaired production of a full-length functional form of a protein or for treating a disease or condition characterized by a defective splicing in a subject. | 2016-01-21 |
20160017323 | CONJUGATED ANTISENSE COMPOUNDS AND THEIR USE - Provided herein are oligomeric compounds with conjugate groups. In certain embodiments, the oligomeric compounds are conjugated to N-Acetylgalactosamine. | 2016-01-21 |
20160017324 | CHIMERIC OLIGOMERIC COMPOUNDS AND THEIR USE IN GENE MODULATION - Oligomer compositions comprising first and second oligomers are provided wherein at least a portion of the first oligomer is capable of hybridizing with at least a portion of the second oligomer, at least a portion of the first oligomer is complementary to and capable of hybridizing to a selected target nucleic acid, and at least one of the first or second oligomers includes at least one nucleotide comprising a chimeric organic composition. Oligomer/protein compositions are also provided comprising an oligomer complementary to and capable of hybridizing to a selected target nucleic acid and at least one protein comprising at least a portion of an RNA-induced silencing complex (RISC), wherein at least one nucleotide comprising a chimeric organic composition. | 2016-01-21 |
20160017325 | LIPOHILLIC OLIGONUCLEOTIDE ANALOGS - Lipophilic oligonucleotide comprising a phosphate glycerol unit containing at least one aliphatic unsaturated carbon bond according to formula (I), with Oligonucleotide an unmodified or modified nucleic acid of 2-1000 nucleotides in length R=a bond or a linker unit Y═OH, SH or NHR3 X and Z=independently O, S or NR3 R3=hydrogen or branched or unbranched and/or substituted or unsubstituted alkyl, aryl and/or alkyl aryl residue with 10 to 30 carbon atoms R1, R2 branched or unbranched and/or substituted or unsubstituted alkyl, aryl and/or alkylaryl residue with 10 to 30 carbon atoms, with the provisio that at least one of the residues R1 or R2 comprises at least one aliphatic carbon-carbon double bond Use of lipophilic oligonucleotide according to Formula I for drug discovery or for transfection of cells. | 2016-01-21 |
20160017326 | MICRORNAS AS FUNCTIONAL MEDIATORS AND BIOMARKERS OF BONE METASTASIS - Pharmaceutical compositions that include therapeutic agents including miRNA nucleic acid sequences are provided. Methods of diagnosing and treating a subjects suffering from a bone degenerative diseases including osteolytic bone metastasis are described. | 2016-01-21 |
20160017327 | PHOSPHORODIAMIDATE MORPHOLINO OLIGOMERS (PMOS) AND THEIR USE IN SUPPRESSION OF MUTANT HUNTINGTIN EXPRESSION AND ATTENUATION OF NEUROTOXICITY - The present invention provides antisense phosphorodiamidate morpholino oligomers which are useful for the suppression or inhibition of the HTT gene involved in Huntington's disease. The oligomers can selectively suppress mutant forms of the HTT protein while allowing the normal protein to be expressed in sufficient quantity to retain its function in the cell. Methods for treatment of Huntington's disease are also provided. | 2016-01-21 |
20160017328 | DOUBLE STRAND COMPOSITIONS COMPRISING DIFFERENTIALLY MODIFIED STRANDS FOR USE IN GENE MODULATION - The present invention provides double stranded compositions wherein each strand is modified to have a motif defined by positioning of β-D-ribonucleosides and sugar modified nucleosides. More particularly, the present compositions comprise one strand having an alternating motif and another strand having a hemimer motif, a blockmer motif, a fully modified motif or a positionally modified motif. At least one of the strands has complementarity to a nucleic acid target. The compositions are useful for targeting selected nucleic acid molecules and modulating the expression of one or more genes. In preferred embodiments the compositions of the present invention hybridize to a portion of a target RNA resulting in loss of normal function of the target RNA. The present invention also provides methods for modulating gene expression. | 2016-01-21 |
20160017329 | OLIGOMERIC COMPOUNDS AND COMPOSITIONS FOR USE IN MODULATION OF SMALL NON-CODING RNAS - Compounds, compositions and methods are provided for modulating the expression and function of small non-coding RNAs. The compositions comprise oligomeric compounds, targeted to small non-coding RNAs. Methods of using these compounds for modulation of small non-coding RNAs as well as downstream targets of these RNAs and for diagnosis and treatment of disease associated with small non-coding RNAs are also provided. | 2016-01-21 |
20160017330 | SERPINC1 iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to iRNA, e.g., double-stranded ribonucleic acid (dsRNA), compositions targeting the Serpinc1 gene, and methods of using such iRNA, e.g., dsRNA, compositions to inhibit expression of Serpinc1 and methods of treating subjects having a bleeding disorder, such as a hemophilia. | 2016-01-21 |
20160017331 | TARGETING MICRORNAS MIR-409-5P, MIR-409-3P AND MIR-154* TO TREAT PROSTATE CANCER BONE METASTASIS AND DRUG RESISTANT LUNG CANCER - The present invention describes methods of treating cancer, cancer metastasis, and drug resistant cancers using miRNA inhibitors; for example, inhibitors of miR-409-5p, miR-409-3p, miR-154*. Also described are methods of using the miRNA as biomarkers; for example, to predict responsiveness to a cancer drug, to detect a disease state of cancer. | 2016-01-21 |
20160017332 | MOLECULES INHIBITING THE HUMAN IMMUNODEFICIENCY VIRUS TYPE 1 (HIV-1), METHOD FOR THE PRODUCTION THEREOF AND APPLICATIONS OF SAME - The invention relates to an aptamer, the structure thereof comprising at least one nucleotide sequence 5′-GGCA(A/G)GGA-3′, that can specifically bind to the poly(A) hairpin of the 5′UTR region of the genome of the human immunodeficiency virus type 1 (HIV-1), providing the method for producing aptamers with said sequence by means of a combination of experimental techniques of in vitro selection of nucleic acids with computational techniques of sequence optimization. The invention also relates to a DNA gene structure for synthesizing said aptamers, preferably RNA. The invention further relates to the different uses of the above-mentioned aptamer, including the use thereof as a biosensor molecule for detecting and/or quantifying HIV-1, as an inhibitor of the production of viral particles of HIV-1, and to the application thereof in medicine, the invention also relating to a method for treating a disease caused by HIV-1, and to a pharmaceutical composition comprising said aptamer. | 2016-01-21 |
20160017333 | DNA APTAMERS FOR PROMOTING REMYELINATION - Materials and methods related to using multimeric DNA aptamers to treat demyelinating diseases are provided herein. | 2016-01-21 |
20160017334 | 5'-TRIPHOSPHATE OLIGORIBONUCLEOTIDES - Disclosed herein are synthetic oligoribonucleotides that form hairpin loop structures. The oligoribonucleotides can be used in the treatment of viral infection including prophylactic treatments. The oligoribonucleotides can also be used as adjuvants. | 2016-01-21 |
20160017335 | PCSK9 iRNA COMPOSITIONS AND METHODS OF USE THEREOF - The invention relates to RNAi agents, e.g., double-stranded RNAi agents, targeting the PCSK9 gene, and methods of using such RNAi agents to inhibit expression of PCSK9 and methods of treating subjects having a lipid disorder, such as a hyperlipidemia. | 2016-01-21 |
20160017336 | COMPOSITIONS AND METHODS FOR INHIBITING EXPRESSION OF FACTOR VII GENE - The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of the Factor VII gene. | 2016-01-21 |
20160017337 | Compositions and Methods for Inhibiting Expression of Eg5 Gene - The invention relates to a double-stranded ribonucleic acid (dsRNA) for inhibiting the expression of the Eg5 gene (Eg5 gene), comprising an antisense strand having a nucleotide sequence which is less that 30 nucleotides in length, generally 19-25 nucleotides in length, and which is substantially complementary to at least a part of the Eg5 gene. The invention also relates to a pharmaceutical composition comprising the dsRNA together with a pharmaceutically acceptable carrier; methods for treating diseases caused by Eg5 expression and the expression of the Eg5 gene using the pharmaceutical composition; and methods for inhibiting the expression of the Eg5 gene in a cell. | 2016-01-21 |
20160017338 | MiRNA molecule defined by its source and its diagnostic and therapeutic uses in diseases or conditions associated with EMT - The invention relates to the diagnostic and therapeutic uses of a miRNA molecule or an equivalent thereof wherein a source of said miRNA molecule or equivalent thereof comprises at least 80 nucleotides and comprises a motif having at least 98% identity with the motif represented by SEQ ID NO:1 or a source thereof in a disease and condition associated with EMT (Epithelial to Mesenchymal Transition). | 2016-01-21 |
20160017339 | NON-CO2 EVOLVING METABOLIC PATHWAY FOR CHEMICAL PRODUCTION - Provided are microorganisms that catalyze the synthesis of chemicals and biochemicals from a suitable carbon source. Also provided are methods of generating such organisms and methods of synthesizing chemicals and biochemicals using such organisms. | 2016-01-21 |
20160017340 | HIGH-THROUGHPUT CARGO DELIVERY INTO LIVE CELLS USING PHOTOTHERMAL PLATFORMS - Methods, devices, and systems are provided for the delivery of agents (e.g., nucleic acids, proteins, organic molecules, organelles, antibodies or other ligands, 5 etc.) into live cells and/or the extraction of the same from said cells. In various embodiments the photothermal platforms and systems incorporating such photothermal platforms are provided that permit efficient, high-throughput cargo delivery into live cells. | 2016-01-21 |
20160017341 | UNIVERSAL PROTEIN OVEREXPRESSION TAG COMPRISING RAMP FUNCTION, AND APPLICATION THEREOF - Provided is a ramp tag capable of solving instability in translation rate resulting from poor compatibility between codons in a foreign gene and a host when expressing a recombinant protein in | 2016-01-21 |
20160017342 | LOW-PHOSPHATE REPRESSIBLE PROMOTER - The present invention provides compositions and methods comprising a low-phosphate repressible promoter. In particular, the present invention provides a low-phosphate repressible promoter from | 2016-01-21 |
20160017343 | HOST CELLS AND METHODS OF USE - The present invention relates to genetically modified host cells, in particular yeast cells, comprising at least one isolated polynucleotide encoding a Killer Expression protease (Kex2p) or a fragment and/or variant thereof which has at least one Kex2p functional activity and at least one isolated polynucleotide encoding a Protein Disulfide-Isomerase (Pdi1) or a fragment and/or variant thereof which has at least one Pdi functional activity. Also provided herein are genetically modified host cells comprising at least one isolated polynucleotide encoding a Killer Expression protease (Kex2p) or a fragment and/or variant thereof which has at least one Kex2p functional activity, at least one isolated polynucleotide encoding a Protein Disulfide-Isomerase (Pdi1) or a fragment and/or variant thereof which has at least one Pdi1 functional activity and at least one isolated polynucleotide encoding a Endoplasmic Reticulum Oxidoreductin (Ero1) or a fragment and/or variant thereof which has at least one Ero1 functional activity. | 2016-01-21 |
20160017344 | THE TELOMERATOR-A TOOL FOR CHROMOSOME ENGINEERING - The present invention relates to the field of chromosome engineering. More specifically, the present invention provides methods and compositions useful for inducibly linearizing circular DNA molecules in vivo in yeast. In one embodiment, a comprises a nucleic acid encoding a selectable marker, wherein the nucleic acid encoding a selectable marker comprises an intron comprising an endonuclease recognition site flanked by telomere seed sequences. | 2016-01-21 |
20160017345 | SELECTABLE MARKER FOR TRANSGENIC PLANTS - Methods of generating transgenic plants containing a gene of interest and a selectable marker encoding an enzyme that catalyzes oxidation of phosphite to phosphate. | 2016-01-21 |
20160017346 | SOYBEAN AGB1 PROMOTER AND ITS USE IN TISSUE-SPECIFIC EXPRESSION OF TRANSGENIC GENES IN PLANTS - The invention relates to gene expression regulatory sequences from soybean, specifically to the promoter of a soybean predicted allergen Gly m Bd 28K peptide gene and fragments thereof and their use in promoting the expression of one or more heterologous nucleic acid fragments in a tissue-specific manner in plants. The invention further discloses compositions, polynucleotide constructs, transformed host cells, transgenic plants and seeds containing the recombinant construct with the promoter, and methods for preparing and using the same. | 2016-01-21 |
20160017347 | TERMINATING FLOWER (TMF) GENE AND METHODS OF USE - Described herein are the following: isolated polynucleotides, isolated polypeptides and recombinant DNA constructs; compositions (such as plants or seeds) comprising these recombinant DNA constructs; and methods of use for these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter that is functional in a plant, wherein said polynucleotide encodes a TMF polypeptide. | 2016-01-21 |
20160017348 | METHODS AND COMPOSITIONS FOR TARGETING RNA POLYMERASES AND NON-CODING RNA BIOGENESIS TO SPECIFIC LOCI - The present disclosure relates to recombinant proteins that induce epigenetic gene silencing and to methods of using such proteins for reducing the expression of genes in plants. | 2016-01-21 |
20160017349 | MAIZE MICRORNA SEQUENCES AND TARGETS THEREOF FOR AGRONOMIC TRAITS - Methods and compositions for maize target gene suppression and improving an agronomic trait through microRNAs or target gene modulation are disclosed. Polynucleotide constructs useful for gene silencing, or upregulation or modulation as well as cells, plants and seeds comprising the polynucleotides and methods for using microRNAs to silence a target gene are also described. | 2016-01-21 |
20160017350 | COMPOSITIONS AND METHODS OF USE OF ACC OXIDASE POLYNUCLEOTIDES AND POLYPEPTIDES - Transgenic compositions and methods reduce the expression of endogenous ACC oxidase genes to improve an agronomic characteristic of a crop plant, which may be maize. Yield increase and drought tolerance due to reduction in the endogenous ACC oxidase levels are observed. ACC oxidase genes are identified in maize, rice, and | 2016-01-21 |
20160017351 | Methods and Compositions for Targeting Sequences of Interest to the Chloroplast - Chimeric polynucleotides comprising a nucleotide sequence encoding a chloroplast transit peptide operably linked to a heterologous polynucleotide of interest are provided, wherein the chloroplast transit peptide comprises an amino acid sequence having the chloroplast transit peptide sequence as set forth in SEQ ID NO: 1 or a biologically active variant or fragment thereof or wherein the chloroplast transit peptide comprises the sequence set forth in SEQ ID NO: 58 or an active variant or fragment thereof. Chimeric polypeptides encoding the same, as well as, cells, plant cells, plants and seeds are further provided which comprise the chimeric polynucleotides. Compositions further include HPPD polypeptides and polynucleotides encoding the same as set forth in SEQ ID NOS: 57 and 60 or active variants and fragments thereof. Such sequences comprise the chloroplast transit peptide as set forth in SEQ ID NO: 58 or an active variants or fragments thereof. Cells, plant cells, plants and seeds are further provided which comprise such sequences. Methods of use of the various sequences are also provided. | 2016-01-21 |
20160017352 | ALGAL STRAIN WITH REDUCED BETA GLUCAN SYNTHASE ACTIVITY - The present invention provides compositions of a modified algal cell and methods of making thereof. In particular, the modified cell has suppressed expression or activity of endogenous beta glucan synthase 1 (BGS1) and increased lipid synthesis when grown under nutrient deficient conditions. | 2016-01-21 |
20160017353 | MODIFIED PROMOTER SEQUENCE AND APPLICATION THEREOF - The present invention relates to a modified plant promoter and applications using the same, including an expression construct comprising the promoter for expressing a gene of interest in a plant expression system. In particular, the present invention relates to a method for producing a polypeptide by expressing a plant cell transformed with the expression construct and recovering the polypeptide from the culture. The present invention also relates to a method for producing a polypeptide by growing a transgenic plant transformed with the expression construct and recovering the polypeptide from the transgenic plant. | 2016-01-21 |
20160017354 | Compositions and Methods for Increasing Methionine Content in Plants - Compositions and methods are provided for increasing methionine content in plants are disclosed. | 2016-01-21 |
20160017355 | TISSUE SPECIFIC REDUCTION OF LIGNIN - The present invention provides an expression cassette comprising a polynucleotide that encodes a protein that diverts a monolignol precursor from a lignin biosynthesis pathway in the plant, which is operably linked to a heterologous promoter. Also provided are methods of engineering a plant having reduced lignin content, as well as plant cells, plant parts, and plant tissues from such engineered plants. | 2016-01-21 |
20160017356 | TRANSCRIPTION FACTORS - The invention provides polynucleotide sequences isolated from plants encoding transcription factors. Polypeptides encoded by the polynucleotides are also provided. Products and methods of use are disclosed. | 2016-01-21 |
20160017357 | POLYNUCLEOTIDES AND METHODS FOR IMPROVING PLANTS - The invention provides methods and compositions for producing plant with altered biomass, the methods comprising the step of altering the expression and/or activity of the polypeptide comprising the sequence of SEQ ID NO:1, or a variant thereof, in a plant cell or plant. The invention also provides a polypeptide comprising the sequence of SEQ ID NO:1, and fragments of variants thereof the sequence. The invention also provides polynucleotides encoding such polypetide sequences. The invention also provides constructs, cells and plants comprising such polynucleotides. | 2016-01-21 |
20160017358 | PLANTS HAVING MODIFIED GROWTH CHARACTERISTICS AND A METHOD FOR MAKING THE SAME - The present invention relates generally to the field of molecular biology and concerns a method for improving various economically important growth characteristics in plants. More specifically, the present invention concerns inter alia a method for modifying growth characteristics in plants by modulating expression in a plant of a nucleic acid encoding a HUB1 (Histone Monoubiquitination 1) polypeptide or encoding another protein useful in the methods of the present invention. The modified growth characteristics comprise a modification of light regulated phenotypes, such as modified circadian clock and/or circadian clock responses, or modified plant architecture. | 2016-01-21 |
20160017359 | PLANTS HAVING ENHANCED YIELD-RELATED TRAITS AND A METHOD FOR MAKING THE SAME - The present invention relates to a method for enhancing various yield-related traits by modulating expression in a plant of a nucleic acid encoding an Ornithine Decarboxylase (ODC) polypeptide, a benzothiadiazole-induced homeodomain 1 (BIHD1) polypeptide, a MYB30, a THOM (tomato homeobox) protein, or a benzothiadiazole-induced homeodomain 2 (BIHD2) polypeptide. The present invention also concerns plants having modulated expression of such a nucleic acid, which plants have enhanced yield-related traits relative to corresponding control plants. The invention also provides constructs useful in the methods of the invention. | 2016-01-21 |
20160017360 | FUNCTIONAL EXPRESSION OF BACTERIAL MAJOR FACILITATOR SUPERFAMILY MFS GENE IN MAIZE TO IMPROVE AGRONOMIC TRAITS AND GRAIN YIELD - Methods for modulating plants using optimized Bacterial MFS constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear tissue growth or kernel number. | 2016-01-21 |
20160017361 | PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING EXOSTOSIN FAMILY POLYPEPTIDES AND HOMOLOGS THEREOF - Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture. | 2016-01-21 |
20160017362 | BIOLOGICAL DEVICES AND METHODS FOR INCREASING THE PRODUCTION OF LYCOPENE FROM PLANTS - Described herein are devices and methods for enhancing the physiological properties of plants. For example, the devices and methods described herein increase the production of lycopene, which has industrial and economic value. The lycopene produced by the devices and methods does not require the ultra purification that is common in conventional or commercial methods. The devices and methods described herein also enhance the growth rate of plants. | 2016-01-21 |
20160017363 | TOXIN GENES AND METHODS FOR THEIR USE - Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:21-74, or the nucleotide sequence set forth in SEQ ID NO: 1-20, as well as variants and fragments thereof. | 2016-01-21 |
20160017364 | PEST RESISTANT PLANTS - The disclosure provides an isolated nucleic acid molecule encoding a 7-epizingiberene synthase, a chimeric gene comprising said nucleic acid molecule, vectors comprising the same, as well as isolated 7-epizingiberene synthase proteins themselves. In addition, transgenic plants and plant cells comprising a gene encoding a 7-epizingiberene synthase, optionally integrated in its genome, and methods for making such plants and cells, are provided. Especially Solanaceae plants and plant parts (seeds, fruit, leaves, etc.) with enhanced insect pest resistance are provided. | 2016-01-21 |
20160017365 | MANIPULATION OF DOMINANT MALE STERILITY - Compositions and methods for modulating male fertility in a plant are provided. Compositions comprise nucleotide sequences, and active fragments and variants thereof, which modulate male fertility. Further provided are expression cassettes comprising the male fertility polynucleotides, or active fragments or variants thereof, operably linked to a promoter, wherein expression of the polynucleotides modulates the male fertility of a plant. Various methods are provided wherein the level and/or activity of the sequences that influence male fertility is modulated in a plant or plant part. In certain embodiments, the plant is polyploid. | 2016-01-21 |
20160017366 | CRISPR-BASED GENOME MODIFICATION AND REGULATION - The present invention provides RNA-guided endonucleases, which are engineered for expression in eukaryotic cells or embryos, and methods of using the RNA-guided endonuclease for targeted genome modification in eukaryotic cells or embryos. Also provided are fusion proteins, wherein each fusion protein comprises a CRISPR/Cas-like protein or fragment thereof and an effector domain. The effector domain can be a cleavage domain, an epigenetic modification domain, a transcriptional activation domain, or a transcriptional repressor domain. Also provided are methods for using the fusion proteins to modify a chromosomal sequence or regulate expression of a chromosomal sequence. | 2016-01-21 |
20160017367 | MGMT-BASED METHOD FOR OBTAINING HIGH YEILDS OF RECOMBINANT PROTEIN EXPRESSION - The present invention relates to a novel enhancer of protein production in host cells. It discloses a vector for expressing recombinant proteins in these cells, comprising a nucleotide sequence encoding a) a secretion peptidic signal, b) a 6-methylguanine-DNA-methyltransferase enzyme (MGMT, EC 2.1.1.63), a mutant or a catalytic domain thereof, and c) a recombinant protein. Said MGMT enzyme is preferably the so-called SNAP protein. | 2016-01-21 |
20160017368 | INORGANIC COATINGS FOR THE ENHANCEMENT OF CHEMICAL TRANSFECTION - Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells with reduced cytotoxicity. The mineral coatings bind biomaterials and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous biomaterial delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform. | 2016-01-21 |