01st week of 2019 patent applcation highlights part 30 |
Patent application number | Title | Published |
20190002915 | COMPOSITIONS AND METHODS FOR REGULATABLE ANTIBODY EXPRESSION - Compositions containing multiple different AAV stock are provided which allow for regulated expression of an immunoglobulin in a variety of tissues. Also provided is a method for regulating the dose of a pharmacologically active immunoglobulin. The method involves co-administering: (a) a first stock of recombinant AAV containing: an activation domain operably linked to expression control sequences comprising a promoter and a first nuclear localization signal; and a DNA binding domain comprising a zinc finger homeodomain and two or more FK506 binding protein domain (FKBP) subunit genes, wherein a first FKBP subunit gene and a second FKBP subunit gene have coding sequences which are no more than about 85% identical to each other, said DNA binding domain being operably linked to a second nuclear localization signal; and (b) a second stock of recombinant AAV comprising at least 2 to about 12 copies of a zinc finger homeodomain which are specific binding partners for the zinc finger homeodomain of the DNA binding domain, and further comprising at least one immunoglobulin expression cassette operably linked to inducible expression control sequences, such that when an effective amount of a rapamycin or rapalog is delivered transcription and expression of the immunoglobulin gene is induced. | 2019-01-03 |
20190002916 | HYBRID DUAL RECOMBINANT AAV VECTOR SYSTEMS FOR GENE THERAPY - The invention relates to constructs, vectors, relative host cells and pharmaceutical compositions which allow an effective gene therapy, in particular of genes larger than 5Kb by using an improved hybrid dual recombinant AAV vector system. | 2019-01-03 |
20190002917 | GENE THERAPY FOR TREATING FAMILIAL HYPERCHOLESTEROLEMIA - Compositions and regimens useful in reducing one or more of: LDL-cholesterol, total cholesterol, and/or fasting triglycerides in a subject, and/or modifying fractional catabolic rate (FCR) of LDL apolipoprotein B (apoB) from baseline to a selected time point after rAAV administration are provided. The method involves administering to the human subject via a peripheral vein by infusion of a suspension of replication deficient recombinant adeno-associated virus (rAAV). | 2019-01-03 |
20190002918 | DONOR PLASMID VECTORS - Certain donor plasmid vectors such as pFastBac™1 and pFastBac™ Dual lack a cis DNA element upstream of the polh translation start codon (ATG) present in wild type (wt) | 2019-01-03 |
20190002919 | In Vivo Methods for Enhancing Bioenergetic Status in Female Germ Cells - In vivo methods using bioenergetic agents for restoring the quality of aged oocytes, enhancing oogonial stem cells or improving derivatives thereof (e.g., cytoplasm or isolated mitochondria) for use in fertility-enhancing procedures, are described. | 2019-01-03 |
20190002920 | METHODS AND KITS FOR CLONING-FREE GENOME EDITING - The methods and compositions provided herein improve upon the methods presently used for targeted genomic modification, in part, by removing the requirement for sub-cloning of a sequence complementary to a site selected for genomic modification. The methods and compositions provided herein can be used in place of a standard CRISPR/Cas system to provide simple, fast, and inexpensive targeted modification of a genome. The methods and compositions provided herein can also be used in high-throughput genome editing applications. | 2019-01-03 |
20190002921 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2019-01-03 |
20190002922 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2019-01-03 |
20190002923 | METHODS AND COMPOSITIONS FOR RNA-DIRECTED TARGET DNA MODIFICATION AND FOR RNA-DIRECTED MODULATION OF TRANSCRIPTION - The present disclosure provides a DNA-targeting RNA that comprises a targeting sequence and, together with a modifying polypeptide, provides for site-specific modification of a target DNA and/or a polypeptide associated with the target DNA. The present disclosure further provides site-specific modifying polypeptides. The present disclosure further provides methods of site-specific modification of a target DNA and/or a polypeptide associated with the target DNA The present disclosure provides methods of modulating transcription of a target nucleic acid in a target cell, generally involving contacting the target nucleic acid with an enzymatically inactive Cas9 polypeptide and a DNA-targeting RNA. Kits and compositions for carrying out the methods are also provided. The present disclosure provides genetically modified cells that produce Cas9; and Cas9 transgenic non-human multicellular organisms. | 2019-01-03 |
20190002924 | System for hydrogen production under limited aerobic conditions - The present invention provides a method for fermentative hydrogen production under limited aerobic conditions by utilizing the respiratory interaction between a strictly anaerobic hydrogen producing bacterium, | 2019-01-03 |
20190002925 | PRODUCTION OF MANOOL - Provided herein are methods of producing (+)-manool comprising: contacting geranylgeranyl diphosphate with an copalyl diphosphate (CPP) synthase to form a (9S, 10S)-copalyl diphosphate wherein the CPP synthase comprises an amino acid sequence having at least 90%, 95%, 98%, 99% and 100% sequence identity to a polypeptide selected from the group consisting of SEQ ID NO: 1 and SEQ ID NO:2; and contacting the CPP with a sclareol synthase enzyme to form (+)-manool. | 2019-01-03 |
20190002926 | METHODS, MATERIALS, SYNTHETIC HOSTS AND REAGENTS FOR THE BIOSYNTHESIS OF HYDROCARBONS AND DERIVATIVES THEREOF - Genetically engineered hosts and methods for their production and use in synthesizing hydrocarbons are provided. | 2019-01-03 |
20190002927 | METHODS, SYNTHETIC HOSTS AND REAGENTS FOR THE BIOSYNTHESIS OF HYDROCARBONS - Systems, networks, methods, compositions and recombinant hosts for biosynthesizing hydrocarbons from a feedstock, such as gas, are provided. | 2019-01-03 |
20190002928 | Grain Processing - The present invention provides a process for producing biogas and/or methane from solid spent cereal products derived from, for example, the mashing process of malt whisk(e)y and/or beer production. There is also provided a system for producing biogas and/or methane from solid spent cereal products derived from, for example, the mashing process of malt whisk(e)y and/or beer production. | 2019-01-03 |
20190002929 | METHOD FOR THE SIMULTANEOUS PRODUCTION OF ETHANOL AND A FERMENTED, SOLID PRODUCT - The invention relates to a method for the simultaneous production of a fermented, solid product and ethanol comprising the following steps: 1) providing a mixture of milled or flaked or otherwise disintegrated biomass, comprising oligosaccharides and/or polysaccharides and live yeast in a dry matter ratio of from 2:1 to 100:1, and water; 2) fermenting the mixture resulting from step (1) under conditions where the water content in the initial mixture does not exceed 65% by weight, for 1-36 hours at a temperature of about 25-60°C. under anaerobic conditions; 3) incubating the fermented mixture resulting from step (2) for 0.5-240 minutes at a temperature of about 70-150°C.; and 4) separating wet fermented, solid product from the fermented mixture resulting from step (3); further comprising either a) that the fermentation in step (2) is performed in one or more interconnected paddle worm or continuous worm conveyers with inlet means for the fermentation mixture and additives and outlet means for the ferment as well as control means for rotation speed, temperature and pH, or b) that one or more processing aids are added in any of steps (1), (2) and (3) and further comprising a step of 5) separating crude ethanol from the fermented mixture in step (2) by vacuum and/or in step (3) by vacuum or by injection of steam and condensing the surplus stripping steam. The invention further relates to the products of this method as well as uses thereof. | 2019-01-03 |
20190002930 | WET OXIDATION OF BIOMASS - A process for producing a transportation fuel from a lignocellulosic feedstock comprising subjecting a stream comprising lignin to a wet oxidation that produces low molecular weight carboxylic acids. These carboxylic acids and/or the corresponding esters are fed to a hydrogenation reaction or gas fermentation wherein they are converted to an alcohol. Heat from the wet oxidation may be supplied to any stage of the process in which heat is introduced. | 2019-01-03 |
20190002931 | METHOD FOR PRODUCING COMPOUND DERIVED FROM HERBACEOUS PLANT OF FAMILY GRAMINEAE OR CUCURBITACEAE - The production method of the present invention includes: a first fermentation step of adding at least one microorganism selected from yeast, | 2019-01-03 |
20190002932 | METHOD FOR SYNTHESISING ORGANIC MOLECULES - Disclosed is a method for synthesising organic molecules from carbon-containing sources and dihydrogen, as well as a device for implementing the method. The method can make use of carbon-containing sources and/or dihydrogen from renewable resources. | 2019-01-03 |
20190002933 | RECOMBINANT YEAST CELLS PRODUCING POLYLACTIC ACID AND USES THEREOF - The present invention relates to a recombinant yeast cell comprising a gene encoding a protein exhibiting lactyl-CoA synthase activity and a gene encoding a protein exhibiting lactyl-CoA polymerase activity, said recombinant cell having the ability of producing polylactic acid (PLA), and the uses thereof. | 2019-01-03 |
20190002934 | TAILORED OILS - Recombinant DNA techniques are used to produce oleaginous recombinant cells that produce triglyceride oils having desired fatty acid profiles and regiospecific or stereospecific profiles. Genes manipulated include those encoding stearoyl-ACP desaturase, delta 12 fatty acid desaturase, acyl-ACP thioesterase, ketoacyl-ACP synthase, and lysophosphatidic acid acyltransferase. The oil produced can have enhanced oxidative or thermal stability, or can be useful as a frying oil, shortening, roll-in shortening, tempering fat, cocoa butter replacement, as a lubricant, or as a feedstock for various chemical processes. The fatty acid profile can be enriched in midchain profiles or the oil can be enriched in triglycerides of the saturated-unsaturated-saturated type. | 2019-01-03 |
20190002935 | BACTERIAL CELLS WITH IMPROVED TOLERANCE TO POLYAMINES - Provided are bacterial cells genetically modified to improve their tolerance to certain commodity chemicals, such as polyamines, and methods of preparing and using such bacterial cells for production of polyamines and other compounds. | 2019-01-03 |
20190002936 | TRANSGENIC MICROORGANISMS AND SYNTHESIS OF PIPERAZIC ACID, PIPERAZIC ACID CONTAINING PRODUCTS, AND DERIVATIVES THEREOF - Among the various aspects of the present disclosure is the provision of a biological and biochemical production of piperazic acid derived from the newly discovered production pathway for L-piperazic acid. One aspect of the present disclosure includes a transgenic microorganism (e.g., bacteria) engineered to accumulate piperazic acid and derivatives thereof, including a piperazic acid (Piz)-containing product. Another aspect of the present disclosure includes biochemical and biological methods for producing piperazic acid and derivatives thereof, including a piperazic acid (Piz)-containing product. Another aspect of the present disclosure includes compositions and methods of using isotopically labeled piperazic acid and derivatives thereof, including a piperazic acid (Piz)-containing product. | 2019-01-03 |
20190002937 | IMPROVED GRANULAR STARCH CONVERSION ENZYMES AND METHODS - Described are methods and compositions relating to granular starch-converting glucoamylases and α-amylases. The enzymes can be used to perform enzymatic starch hydrolysis of granular starch at or below the gelatinization temperature of insoluble granular starch. | 2019-01-03 |
20190002938 | PROCESS FOR PRODUCING GELLAN GUM - The invention provides a new fermentation process for producing gellan gum, which can control the feeding amount and feeding speed of the nitrogen sources accurately and quantitatively, and control the growth and gum production of the strains, so as to significantly improve the controllability, stability and yield of the fermentation process for producing gellan gum. | 2019-01-03 |
20190002939 | Highly Efficient Method for Synthesizing Difructose Anhydride III - The invention discloses a high-efficiency synthesis method of difructose anhydride III. The method comprises the following steps: firstly converting sucrose into inulin by using inulosucrase without separating polysaccharide, and then converting inulin by using inulin fructotransferase to synthesize the functional disaccharide difructose anhydride III. The method has the advantages of simple process and high efficiency, and a conversion rate of synthesizing inulin into difructose anhydride III can reach 40%-54%. In order to obtain purer difructose anhydride III, yeast is utilized to remove small molecule monosaccharides in a reaction solution, and the finally obtained purer difructose anhydride III can be easily separated and purified. Thus, the method has broad market application prospects. | 2019-01-03 |
20190002940 | USING DISSOLVED OXYGEN TO INHIBIT LACTIC ACID PRODUCTION DURING PROPAGATION OF YEAST AND/OR HYDROLYSIS OF LIGNOCELLULOSIC BIOMASS - Embodiments of the present disclosure involve systems and methods that inhibit the production of lactic acid during propagation of yeast and/or during hydrolysis of cellulose by including a sufficient amount of dissolved oxygen. | 2019-01-03 |
20190002941 | Cellulolytic enzyme compositions and uses thereof - The present invention relates to recombinant filamentous fungal host cells producing cellulolytic enzyme compositions and methods of producing and using the compositions. | 2019-01-03 |
20190002942 | ANTIBIOTIC FIIRV 104/18 COMPLEX AND THE ISOLATED INDIVIDUAL FACTORS THEREOF - An antibiotic FIIRV 104/18 complex comprising factors F793, F795, F797, F813 and F683, of formula (I), wherein R represents a glycosidic radical as defined in the specification, the isolated individual factors, a method for their production by cultivation of | 2019-01-03 |
20190002943 | SYSTEM AND METHOD FOR SYNTHESIS OF DNA PARTICLES AND USE THEREOF - Disclosed is a system and method for production of DNA particles and use thereof. The DNA particles can be produced by amplification of nucleic acid molecule(s). Alternatively, DNA particles can be prepared by condensing multiple DNA molecules. The DNA condensation into a particle is mainly triggered by pyrophosphate and positively charged cations (e.g. magnesium). DNA particles can be applied for numerous biological applications but not limited to directed evolution, proteomics, drug delivery and imaging. DNA particles can be used to synthesize proteins using in vitro transcription/translation reaction. | 2019-01-03 |
20190002944 | ACOUSTIC ENERGY MEDIATION OF GENETIC FRAGMENTATION - Method and apparatus for controlling acoustic treatment of a sample to mediate a tagmentation process used on double stranded DNA. | 2019-01-03 |
20190002945 | GENERATION AND COMPARATIVE KINETIC ANALYSIS OF NEW GLYCOSYNTHASE MUTANTS FROM STREPTOCOCCUS PYOGENES FNDOGLYCOSIDASES FOR ANTIBODY GLYCOENGINEERING - The present invention provides for recombinant Endo-S mutants (named Endo-S glycosynthases) that exhibit reduced hydrolysis activity and increased transglycosylation activity for the synthesis of glycoproteins wherein a desired sugar chain is added to a fucosylated or nonfucosylated GlcNAc-IgG acceptor. As such, the present invention allows for the synthesis and remodeling of therapeutic antibodies thereby providing for certain biological activities, such as, prolonged half-life time in vivo, less immunogenicity, enhanced in vivo activity, increased targeting ability, and/or ability to deliver a therapeutic agent. | 2019-01-03 |
20190002946 | METHODS FOR INCREASING MANNOSE CONTENT OF RECOMBINANT PROTEINS - The present invention relates to methods of upregulating the high mannose glycoform content of a recombinant protein during a mammalian cell culture by manipulating the mannose to total hexose ratio in the cell culture media formulation. | 2019-01-03 |
20190002947 | RECOMBINANT PRODUCTION METHOD - Herein is reported a method for the recombinant production of a polypeptide in a eukaryotic cell comprising the steps of (i) cultivating a eukaryotic cell comprising a nucleic acid encoding the polypeptide in a cultivation medium comprising a compound selected from the group consisting of trans-2-methyl 2-pentenoic acid, the broad-spectrum HDAC inhibitor Quisinostat, and the subtype-specific HDAC inhibitor Romidepsin, and (ii) recovering the polypeptide from the cell or the cultivation medium and thereby producing the polypeptide in a eukaryotic cell. | 2019-01-03 |
20190002948 | METHOD AND ELECTRONIC DEVICE FOR DETERMINING THE CONCENTRATION OF AN ANALYTE - A method is provided for determining, the presence and concentration of an analyte by contacting said sample with a solution comprising: magnetic beads, a capture probe capable of binding said analyte, a reporter probe and cellulose, whereby, if the analyte is present, an MB-analyte-reporter-cellulase sandwich is formed; and then contacting said solution comprising said sandwich with an electrode covered with an electrically insulating layer comprising or consisting of cellulose and/or a cellulose derivative, wherein the MB-analyte-reporter-cellulase sandwich leads to degradation of the insulating layer thereby causing a measurable change in electrical properties at the electrode surface, wherein said change in electrical properties is a function of the amount of analyte in said sample. Devices and biosensor applying the method are also provided. | 2019-01-03 |
20190002949 | COMPOSITIONS AND METHODS FOR MEASURING BLOOD GLUCOSE LEVELS - In some embodiments, the present invention a mutated FAD-GDHa protein, wherein the mutated FAD-GDHa protein is mutated from a wild-type first species to contain at least one point mutation, wherein the mutated FAD-GDHα protein comprises: P(X) | 2019-01-03 |
20190002950 | METHOD AND SYSTEM FOR IMAGING A BLOOD SAMPLE - Apparatus and methods are described including introducing a cell suspension comprising red blood cells into a carrier that is a closed cavity that includes a base surface, via an inlet defined by the carrier. The cells in the cell suspension are allowed to settle on the base surface of the carrier to form a monolayer of cells on the base surface of the carrier. At least one microscope image of at least a portion of the monolayer of cells is acquired. Other applications are also described. | 2019-01-03 |
20190002951 | SYSTEMS AND METHODS FOR CONFIRMING ACTIVATION OF BIOLOGICAL INDICATORS - Biological indicators may be improperly activated. The disclosed subject matter is directed to methods of confirming that a biological indicator having an ampule containing a growth medium has been properly activated such that it may be assayed. The methods may include the steps of measuring a first fluorescence intensity of the biological indicator, heating the biological indicator; quenching the fluorescence intensity of the biological indicator from the first fluorescence intensity to a second fluorescence intensity, measuring the second fluorescence intensity; comparing the second fluorescence intensity and first fluorescence intensity to obtain a comparison value; and determining that the comparison value corresponds to a quenching metric of the liquid growth medium. | 2019-01-03 |
20190002952 | COMPOSITIONS AND METHODS FOR DIAGNOSIS OF SHOCK - Methods and kits for diagnosis and staging of shock, and especially non-septic shock are presented in which protease activities and/or volatile compounds are measured from a biological sample to so identify and/or stage shock. | 2019-01-03 |
20190002953 | A NOVEL METHOD FOR THE PREPARATION OF BAR-CODED PRIMER SETS - The present invention relates to a method of producing a set of primers suitable for the reverse transcription and/or amplification of a plurality (N) of nucleic acid molecules of interest, wherein for each nucleic acid molecule of interest at least one primer is produced and wherein the primers carry a bar-code, the method comprising the steps of: (a)(i) combining (1) a first oligonucleotide, wherein said first oligonucleotide comprises a first bar-code nucleic acid sequence linked at its 3′ end to a first adapter nucleic acid sequence with (2) a plurality (N) of second oligonucleotides, wherein each second oligonucleotide comprises the reverse complementary sequence of a forward primer specific for a nucleic acid molecule of interest, wherein said reverse complementary sequence of the forward primer is linked at its 3′ end to the reverse complementary sequence of the first adapter nucleic acid sequence; and/or (a)(ii) combining (1) a third oligonucleotide, wherein said third oligonucleotide comprises a second bar-code nucleic acid sequence linked at its 3′ end to a second adapter nucleic acid sequence with (2) a plurality (N) of fourth oligonucleotides, wherein each fourth oligonucleotide comprises the reverse complementary sequence of a reverse primer specific for said nucleic acid molecule of interest, wherein said reverse complementary sequence of the reverse primer is linked at its 3′ end to the reverse complementary sequence of the second adapter nucleic acid sequence; wherein steps (a)(i) and (a)(ii) are carried out under conditions that enable the annealing of the first and second adapter nucleic acid sequences to the respective reverse complementary sequences thereof; (b) extending the oligonucleotides of (a)(i) and (a)(ii) by polymerase-mediated oligonucleotide synthesis; and (c) optionally, removing the second and fourth oligonucleotides. The present invention further relates to methods of producing a plurality (M) of nucleic acid amplification products of interest carrying at least one sample-specific bar-code as well as to a method for multiplex sequencing of a plurality (M) of nucleic acid amplification products of interest from a plurality (X) of samples in a single reaction chamber and identifying the individual sample from which each nucleic acid amplification product is derived. Furthermore, the present invention relates to a target-unspecific bar-code-adapter panel, its use in the methods of the invention as well as a kit comprising same. | 2019-01-03 |
20190002954 | EXTREME REVERSE TRANSCRIPTION PCR - Methods, kits and mixtures are provided for performing RT-PCR with an RT incubation of no more than one minute and PCR cycles in <20 seconds per cycle. | 2019-01-03 |
20190002955 | APPARATUS FOR AMPLIFICATION OF NUCLEIC ACIDS - Described herein is a chip-based apparatus for amplifying nucleic acids, a cartridge housing the apparatus, and methods of using the apparatus for amplification of nucleic acids. More specifically, this invention provides integrated semiconductor chip, manufactured with standard semiconductor manufacturing process, with on-chip circuitry to perform thermal management and optical sensing necessary for amplification of nucleic acids. The apparatus and methods embodied in this invention makes it possible to build a disease diagnosis and prognosis tool that is easy to use, portable and disposable. | 2019-01-03 |
20190002956 | SYSTEM AND METHOD FOR DROPLET DETECTION - Systems and methods for detection of a signal from droplets of an emulsion. An exemplary system may comprise a fluid transporter having a tube with an open end for aspirating droplets, a singulator to arrange the droplets in single file and to space the single-file droplets from one another, and a detection channel in optical communication with a detector configured to detect a signal from droplets. In some embodiments, the singulator may have a channel junction at which a stream of droplets in single file is combined with a stream of spacing fluid, and a tapered spacing channel extending downstream from the channel junction toward the detection channel. In some embodiments, the fluid transporter may suck droplet-containing fluid and spacing fluid through the detection channel from respective sources. In some embodiments, droplets may be subjected to a disaggregation routine before they are passed through the detection channel. | 2019-01-03 |
20190002957 | NUCLEIC ACID AMPLIFICATION - The present invention provides methods for the amplification of nucleic acid molecules. Methods for amplifying target polynucleotides, including mRNA, using oligonucleotides, DNA and RNA polymerases are provided. The invention further provides compositions and kits for practicing the methods, as well as methods which use the amplification products. | 2019-01-03 |
20190002958 | MODULATION OF ACCESSIBILITY OF HOST NUCLEIC ACIDS TO NUCLEIC ACID DIGESTING ENZYMES IN ACELLULAR BIOLOGICAL FLUIDS - Disclosed is a method for isolating, amplifying, and sequencing infectious agents' nucleic acids from an acellular fraction of a biological fluid using detergents and nucleic acids-digesting enzymes. Also disclosed is a kit-of-parts including detergents and nucleic acids-digesting enzymes for the implementation of the methods described. | 2019-01-03 |
20190002959 | SYSTEM AND METHOD FOR PROCESSING BIOLOGICAL SAMPLES - A system and method for processing and detecting nucleic acids from a set of biological samples, comprising: a molecular diagnostic module configured to receive nucleic acids bound to magnetic beads, isolate nucleic acids, and analyze nucleic acids, comprising a cartridge receiving module, a heating/cooling subsystem and a magnet configured to facilitate isolation of nucleic acids, a valve actuation subsystem including an actuation substrate, and a set of pins interacting with the actuation substrate, and a spring plate configured to bias at least one pin in a configurations, the valve actuation subsystem configured to control fluid flow through a microfluidic cartridge for processing nucleic acids, and an optical subsystem for analysis of nucleic acids; and a fluid handling system configured to deliver samples and reagents to components of the system to facilitate molecular diagnostic protocols. | 2019-01-03 |
20190002960 | GENETIC TESTING FOR ALIGNMENT-FREE PREDICTING RESISTANCE OF MICROORGANISMS AGAINST ANTIMICROBIAL AGENTS - The present invention relates to a method of determining an infection of a patient with at least one microorganism, particularly a bacterial microorganism, potentially resistant to antimicrobial drug treatment, a method of selecting a treatment of a patient suffering from an infection with at least one microorganism, particularly bacterial microorganism, and a method of determining an antimicrobial drug, e.g. antibiotic, resistance profile for at least one microorganism, particularly bacterial microorganism, as well as computer program products used in these methods. | 2019-01-03 |
20190002961 | DETECTION OF NEISSERIA GONORRHOEAES - Methods and compositions for detection of | 2019-01-03 |
20190002962 | GENE-MATCHED ENRICHMENT AND POLYMERASE CHAIN REACTION FOR RAPID DETECTION OF MICROORGANISMS - A method for amplifying and detecting microorganisms, such as species of Listeria, is described. The method utilizes gene-matched enrichment media and PCR-based detection. The enrichment media is spent media produced using a modified microorganism containing a plurality of mutations in a selected gene such that the modified microorganism does not contain the PCR signature. Thus, PCR detects only the amplified microorganism of interest, not the modified microorganism. Exemplary methods and kits for amplification and detection of Listeria species are described. | 2019-01-03 |
20190002963 | MULTIPLEXED FLUOROMETRIC MEASUREMENTS WITH DROPLET PCR SYSTEMS - The present disclosure provides methods and compositions for detection of multiple nucleic acid targets in a single optical channel in a digital assay. In some cases, three or more nucleic acid targets may be detected in a single channel. Nucleic acid targets may be partitioned, amplified, used to generate signals, where each signal corresponds to a unique combination of nucleic acid targets in a partition. | 2019-01-03 |
20190002964 | BODILY FLUID TARGET ENRICHMENT - The invention provides methods for capturing target nucleic acid directly from bodily fluid samples, without the need for certain complex sample preparation steps, using Cas endonuclease to bind to the target nucleic acid sequences. The Cas proteins, along with their sequence-specific guide RNAs, may be introduced directly into the sample, where the Cas proteins bind to ends of a target nucleic acid. The target nucleic acid is thus isolated or enriched in a sequence-specific manner. The target nucleic acid may then be subject to any suitable detection or analysis assay, such as amplification or sequencing. The target nucleic acid may be enriched by digesting other, unbound nucleic acids present in the sample with exonuclease. The bound Cas proteins prevent exonuclease from digesting the target nucleic acid, thereby leaving the only the target nucleic acid substantially present in the sample. | 2019-01-03 |
20190002965 | AMPLIFICATION AND ANALYSIS OF SELECTED TARGETS ON SOLID SUPPORTS - Methods are provided for multiplexed amplification of selected targets and analysis of the amplified targets. In preferred aspects the amplification and analysis take place on the same solid support and preferably in a localized area such as a bead or a feature of an array. Targets are circularized by hybridization to probes followed by ligation of the ends of the target to form a closed circle. The targets are then used as template for extension of an array bound probe resulting in extended probes having multiple copies of the target. The extended probes can then be analyzed. The methods may be used for genotyping, sequencing and analysis of copy number. | 2019-01-03 |
20190002966 | DETECTION OF CHROMOSOME ABNORMALITIES - Embodiments of the present invention provide a computer-implemented method of determining a probability of a fetal chromosomal abnormality, the method comprising determining data indicative of a first parameter for a target chromosome and a second parameter representative of chromosome sequence density from a biological sample obtained from a female subject, determining a likelihood ratio indicative of fetal chromosomal abnormality, wherein the likelihood ratio is determined as a ratio between a probability of chromosomal abnormality and a probability of chromosomal normality according to respective abnormality and normality models based on the first and second parameters, determining one or more performance parameter thresholds, and comparing an estimate of one or more performance parameters associated with the sample against the one or more performance parameter thresholds. | 2019-01-03 |
20190002967 | Methods of Amplifying Nucleic Acid Sequences - Methods are provided for nucleic acid amplification including contacting a double stranded nucleic acid with transposases bound to transposon DNA, wherein the transposon DNA includes a transposase binding site and an RNA polymerase promoter sequence, wherein the transposases/transposon DNA complex bind to target locations along the double stranded nucleic acid and cleave the double stranded nucleic acid into a plurality of double stranded fragments, with each double stranded fragment having the transposon DNA bound to each 5′ end of the double stranded fragment, extending the double stranded fragments along the transposon DNA to make double stranded extension products having double stranded RNA polymerase promoter sequences at each end, contacting the double stranded extension products with an RNA polymerase to make a plurality of RNA transcripts of each double stranded extension product, reverse transcribing the RNA transcripts into single stranded copy DNA, forming complementary strands to the single stranded copy DNA to form a plurality of double stranded DNA amplicons corresponding to each double stranded fragment. | 2019-01-03 |
20190002968 | POLYPEPTIDE TAGGED NUCLEOTIDES AND USE THEREOF IN NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION - The present disclosure relates to compositions and methods based on polypeptide-tagged nucleotide, and the use of such polypeptide-tagged nucleotides in nanopore devices and methods. | 2019-01-03 |
20190002969 | MULTIPLE TAGGING OF LONG DNA FRAGMENTS - The present invention provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example. | 2019-01-03 |
20190002970 | MULTIPLE TAGGING OF INDIVIDUAL LONG DNA FRAGMENTS - This disclosure provides methods and compositions for tagging long fragments of a target nucleic acid for sequencing and analyzing the resulting sequence information in order to reduce errors and perform haplotype phasing, for example. | 2019-01-03 |
20190002971 | OPTICALLY-BASED NANOPORE ANALYSIS WITH REDUCED BACKGROUND - The invention is directed to nanopore arrays comprising opaque layers that reduce background fluorescence in optical signal collected in applications of such arrays for analyzing molecules. In some embodiments, such arrays are used to determine characteristics of polymers, such as polynucleotides, in methods comprising the steps of translocating polymers through nanopores of such arrays wherein polymers have one or more optical labels, exciting optical labels of the polymers in a signal generation region of each nanopore extending from the opaque layer toward the direction of the excitation beam, detecting optical signals from the signal generation regions of each nanopore to determine characteristics of the polymer translocating therethrough. | 2019-01-03 |
20190002972 | ENZYME-PORE CONSTRUCTS - The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing. | 2019-01-03 |
20190002973 | APPARATUS AND METHODS FOR KINETIC ANALYSIS AND DETERMINATION OF NUCLEIC ACID SEQUENCES - A method of distinguishing nucleotide sequences for different nucleic acid molecules including the steps of (a) mixing a plurality of different nucleic acid molecules with polymerase molecules and nucleotide molecules, wherein the different nucleic acid molecules are attached to a surface in the form of an array of nucleic acid features; (b) determining a transient state of the polymerase molecules at the nucleic acid features; and (c) identifying a subset of nucleic acid features that correctly incorporate the nucleotide molecules based on the transient state of the polymerase molecules at the nucleic acid features, thereby distinguishing the nucleotide sequences for the different nucleic acid molecules. | 2019-01-03 |
20190002974 | System and Methods for Massively Parallel Analysis of Nucleic Acids in Single Cells - Methods and systems are provided for massively parallel genetic analysis of single cells in emulsion droplets or reaction containers. Genetic loci of interest are targeted in a single cell using a set of probes, and a fusion complex is formed by molecular linkage and amplification techniques. Methods are provided for high-throughput, massively parallel analysis of the fusion complex in a single cell in a population of at least 10,000 cells. Also provided are methods for tracing genetic information back to a cell using barcode sequences. | 2019-01-03 |
20190002975 | Methods and Systems for High-throughput Toxicity Screening of a Compound Using Mahalanobis Values - The invention relates to methods and systems for high-throughput toxicity screening of compounds using Mahalanobis Values, and in particular comparing a normal unexposed transcriptome Mahalanobis Value in an in-vitro hepatocyte microassay against a calculated transcriptome Mahalanobis Value of hepatocytes exposed to a target compound for varying time periods and in varying concentrations. | 2019-01-03 |
20190002976 | TEST FOR PREDICTING THE ABILITY OF A SALMONID TO UTILISE DIETARY PIGMENT - The present invention relates to a method of predicting the ability of a salmonid to utilise dietary pigment, the method comprising determining the alleles present at one or more DNA polymorphism in the abcg 2 gene in the salmonid and predicting the ability of the salmonid to utilise dietary pigment based on the determination of the alleles. Such a method may be used in a method of selecting a salmonid for use as broodstock. The present invention also relates to a method of genome editing in order to create a salmonid with an increased ability to utilise dietary pigment, the method comprising editing the genome of the salmonid in order to introduce one or more red allele of a DNA polymorphism in the abcg2 gene. | 2019-01-03 |
20190002977 | Growth Differentiation Factor 15 as Biomarker for Metformin - The present invention relates to metformin for use in treating a patient, wherein the patient exhibits an increased level of GDF15 in response to metformin treatment; to methods of identifying a patient who will benefit or who will not benefit from metformin treatment; methods of treating a patient at risk of developing or suffering from a disease or disorder comprising administering therapeutically effective amount of metformin; methods of adapting the dosage of metformin; the usage of GDF15 as biomarker for identifying a patient who will benefit or who will not benefit from metformin treatment, kits for use in identifying a patient who will benefit from metformin treatment and the use of the kits, as well as methods of treating a patient or who will not benefit from metformin treatment. | 2019-01-03 |
20190002978 | PREGNANCY ASSOCIATED GLYCOPROTEIN (PAG) GENES AS MARKERS OF BULL FERTILITY - A method for selecting for enhanced fertility of a male mammal includes obtaining one or more samples of a cell or tissue from a plurality of male mammals and quantifying one or both of a blood pregnancy-associated glycoprotein (PAG) concentration or a PAG genomic DNA in the one or more samples of a cell or tissue. Male mammals of the plurality of male mammals exhibiting a highest circulating PAG and/or a highest PAG genomic DNA are selected. Cells/tissues from the selected male mammals may be utilized in a reproductive procedure. Kits for accomplishing the methods are provided. | 2019-01-03 |
20190002979 | HAPLOTYPING OF HLA LOCI WITH ULTRA-DEEP SHOTGUN SEQUENCING - Methods are provided to determine the entire genomic region of a particular HLA locus including both intron and exons. The resultant consensus sequences provides linkage information between different exons, and produces the unique sequence from each of the two genes from the individual sample being typed. The sequence information in intron regions along with the exon sequences provides an accurate HLA haplotype. | 2019-01-03 |
20190002980 | COMPOSITIONS AND METHODS FOR ENHANCING ODORANT RECEPTOR ACTIVITY - The present invention relates to polypeptides capable of modulating odorant receptor activation. In particular, the present invention provides polypeptides (e.g., type 3 muscarinic actetylcholine receptor M3) capable of enhancing odorant receptor activation. The present invention further provides assays for the detection of ligands specific for various odorant receptors. Additionally, the present invention provides methods of screening for polypeptide polymorphisms and mutations associated with odorant receptor activation (e.g., polymorphisms and mutations associated with muscarinic actetylcholine receptor polypeptides (e.g., M1, M2, M3, M4, M5)), as well as methods of screening for therapeutic agents, ligands, and modulators of such proteins. | 2019-01-03 |
20190002981 | Method of Testing for Preeclampsia and Treatment Therefor - The present invention relates to novel genetic markers associated with preeclampsia and risk of developing preeclampsia, and methods and materials for determining whether a human subject has preeclampsia or is at risk of developing preeclampsia and the use of such risk information in selectively administering a treatment that at least partially prevents or compensates for an preeclampsia related condition. | 2019-01-03 |
20190002982 | Systems For Biomarker Detection - Biomarker associated with Alzheimer's Disease (AD) and/or Mild Cognitive Impairment (MCI), where the biomarker is an intracellular or circulating microRNA and/or target protein thereof, and use thereof in methods for determining, diagnosing, monitoring AD, mild AD, moderate Ad, severe AD, MCI, early MCI, late MCI, and the like, as well as in methods for selecting candidate therapeutic compounds for treating same. | 2019-01-03 |
20190002983 | SERUM MIRNA MARKER FOR OPLL DIAGNOSIS AND APPLICATION THEREOF - A serum microRNA (miRNA) marker suitable for early screening and diagnosis of ossification of posterior longitudinal ligament (OPLL) and its application in the diagnostic reagent or kit for the OPLL. Biomarker miRNA-563, miRNA-196b, miRNA-10a and miRNA-129 have high diagnostic value for OPLL, and the development and application of the related serum miRNA biomarker detection reagent kit. It can be applied in the screening of ossification of posterior longitudinal ligament disease, supporting the diagnosis of OPLL more quickly and accurately, evaluate the patient's ossification condition, and may lay down the foundation for improving clinical therapeutic effect. | 2019-01-03 |
20190002984 | SEROTONIN TRANSPORTER GENE AND TREATMENT OF ALCOHOLISM - The gene responsible for encoding SERT has a functional polymorphism at the 5′-regulatory promoter region, which results in two forms, long (L) and short (S). The LL-genotype is hypothesized to play a key role in the early onset of alcohol use. The present invention discloses the differences in treatment and diagnosis based on the L or short genotypes as well as on a single nucleotide polymorphism of the SERT gene, the 3′ UTR SNP rs1042173. The present invention demonstrates the efficacy of using the drug ondansetron and similar drugs for treatment based on variations in the polymorphisms of the SERT gene as well as methods for diagnosing susceptibility to abuse of alcohol and other addiction-related diseases and disorders. | 2019-01-03 |
20190002985 | METHODS FOR DETERMINING THE QUALITY OF AN EMBRYO - The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to in vitro non invasive methods and kits for determining the quality of an embryo by determining the level of the cell free nucleic acids and/or determining the presence and/or expression level of at least one specific nucleic acid sequence in the nucleic acid extraction. | 2019-01-03 |
20190002986 | Method to risk-stratify patients with cancer based on the comorbidities, and related differential gene expression information - A method to risk-stratify patients with cancer based on the comorbidities, and related differential gene expression is described here. This is of relevance when counseling and treating patients with various malignancies. | 2019-01-03 |
20190002987 | DETECTION OF TUMOR-DERIVED DNA IN CEREBROSPINAL FLUID - As cell-free DNA from brain and spinal cord tumors cannot usually be detected in the blood, we assessed the cerebrospinal fluid (CSF) that bathes the CNS for tumor DNA, here termed CSF-tDNA. The results suggest that CSF-tDNA could be useful for the management of patients with primary tumors of the brain or spinal cord. | 2019-01-03 |
20190002988 | METHOD OF USING A RET FUSION GENE AS A BIOMARKER TO SELECT NON SMALL CELL LUNG CANCER (NSCLC) AND THYROID CANCER PATIENTS FOR A CANCER TREATMENT - The present invention relates to a RET fusion gene such as a CCDC6-RET fusion gene as a biomarker to monitor the activity of the compound 3-Z-[1-(4-(N-((4-methyl-piperazin-1-yl)-methylcarbonyl)-N-methyl-amino)-anilino)-1-phenyl-methylene]-6-methoxycarbonyl-2-indolinone or a pharmaceutically acceptable salt thereof, and especially its monoethanesulphonate salt form, when used alone or optionally in combination with further pharmaceutically active ingredients and/or further treatments. The present invention also relates to specific uses of said specific compound in the treatment of cancers. | 2019-01-03 |
20190002989 | MCL-1 AS A THERAPEUTIC TARGET IN SCFFBW7 DEFICIENT NEOPLASM - Some embodiments are based on the discovery that proliferative diseases (e.g., neoplastic diseases, for example, tumors or cancers) having an FBW7 mutation or other FBW7 deficiency are sensitive to Mc11 inhibiting agents, but resistant to pro-apoptotic drugs that do not inhibit Mc11. Some embodiments provide methods of treating a proliferative disease based on an assessment of FBW7 expression level or mutation status. Some embodiments provide methods of classifying a hyperproliferative cell or cell population, for example, a malignant cell or cell population based on an assessment of FBW7 expression level or mutation status. Some embodiments provide methods of selecting a treatment regimen for treating a proliferative disease, for example, a malignant disorder, based on an assessment of FBW7 expression level or mutation status. | 2019-01-03 |
20190002990 | COMPOSITIONS AND METHODS FOR CHARACTERIZING A DNA REPAIR VARIANT POLYPEPTIDE - As described below, the present invention provides quantitative homologous recombination assays developed to characterize the pathogenicity DNA repair polypeptides (e.g., BRCA1, BRCA2, Rad51) and provide urgently needed functional information on the significance of DNA repair variants of uncertain significance (VUS) alleles. The invention also provides a method of generating site-specific recombination at a genomic locus or site-specific genome editing by inhibiting replication at the genomic locus, e.g., involving contacting the genomic locus with polypeptides that specifically bind target sequences at the genomic locus. | 2019-01-03 |
20190002991 | METHOD FOR DIAGNOSING AND TREATING OVARIAN CANCER - Provided are a composition, a kit, and a method of predicting prognosis of ovarian cancer or a risk of recurrence of ovarian cancer. Provided are a composition for treating ovarian cancer or preventing recurrence of ovarian cancer and a method of screening a material for treating ovarian cancer or preventing recurrence of ovarian cancer. According to the present disclosure, prognosis or recurrence of ovarian cancer can be efficiently diagnosed, and a candidate material that can treat ovarian cancer or prevent recurrence of ovarian cancer can be efficiently screened. | 2019-01-03 |
20190002992 | GENETIC TESTING FOR IMPROVED CATTLE FERTILITY - Arrays of nucleic acid molecules, kits, methods of genotyping and marker assisted bovine breeding methods based on novel SNPs on genes of the bovine transforming growth factor-β (TGF-β) signaling pathway for improved bovine fertilization rate. The methods and compositions of the present invention are related to SNPs in the DNA-binding protein inhibitor 3 (ID3) gene, and in the bone morphogenetic protein 4 (BMP4) gene corresponding to position 2702 of SEQ ID NO: 2. Also disclosed are methods for determining viability of developing bovine embryos by measuring the expression level of one or more target genes in the TGF-signaling pathway, and selecting for implantation only embryos whose target gene expression level is not up-regulated. | 2019-01-03 |
20190002993 | METHODS AND COMPOSITIONS FOR DETECTING CANDIDA SPECIES - Disclosed are methods utilizing specific amplification of | 2019-01-03 |
20190002994 | PRIMERS FOR DETECTING INFLUENZA BY USING LAMP, AND USE THEREOF - Disclosed are: a primer set enabling the specific detection, by an isothermal amplification method, of an influenza A virus, an influenza A subtype H3 virus, an influenza A subtype pdm H1N1 virus, and an influenza B virus; a composition or a kit comprising the same; and a method for detecting influenza viruses by using the same. The primers and the method, according to the present application, can detect, in a rapid manner and with high sensitivity and specificity, whether influenza virus infection occurs, and enable detection without separate treatments after the completion of the amplification, thereby improving convenience. | 2019-01-03 |
20190002995 | DETECTION OF NUCLEIC ACIDS FROM MULTIPLE TYPES OF HUMAN PAPILLOMAVIRUS - Nucleic acid oligonucleotide sequences are disclosed which include amplification oligomers and probe oligomers which are useful for detecting multiple types of human papillomaviruses (HPV) associated with cervical cancer. Methods for detecting multiple HPV types in biological specimens by amplifying HPV nucleic acid sequences in vitro and detecting the amplified products are disclosed. | 2019-01-03 |
20190002996 | PLANT AND METHOD FOR VACUUM DEGASSING LIQUID STEEL - The invention relates to a plant for vacuum degassing liquid steel, comprising: at least one vacuum chamber | 2019-01-03 |
20190002997 | METHOD AND SYSTEM FOR LASER HARDENING OF A SURFACE OF A WORKPIECE - A method of laser hardening of a surface area of a workpiece, such as a surface of a journal of a crankshaft, including the steps of generating a relative movement between the surface of the workpiece and a laser source to allow a laser spot to subsequently be projected onto different portions of the surface area, and during the relative movement, repetitively scanning the laser beam so as to produce a two-dimensional equivalent effective laser spot on the surface area. The energy distribution of the effective laser spot is adapted so that it is different in a more heat sensitive subarea, such as in an area adjacent to an oil lubrication opening, than in a less heat sensitive subarea, so as to prevent overheating of the more heat sensitive subarea. | 2019-01-03 |
20190002998 | Low Alloy Steels with Enhanced Toughness and Fatigue Strength at High Hardness - Methods of forming low alloy steels and steels produced by such methods are provided. Various alloy additions and elements, as well as heating and tempering times and method steps are provided herein. Methods and materials of the present disclosure provide for enhanced fatigue at high hardness as compared with more brittle conventional steels. | 2019-01-03 |
20190002999 | CASE HARDENING STEEL, CARBURIZED COMPONENT, AND MANUFACTURING METHOD OF CASE HARDENING STEEL - A case hardening steel includes, as a chemical composition, by mass %, C: 0.10% to 0.30%, Si: 0.02% to 1.50%, Mn: 0.30% to 1.80%, S: 0.003% to 0.020%, Cr: 0.40% to 2.00%, Al: 0.005% to 0.050%, Ti: 0.06% to 0.20%, Bi: 0.0001% to 0.0050%, Mo: 0% to 1.50%, Ni: 0% to 3.50%, V: 0% to 0.50%, B: 0% to 0.0050%, Nb: 0% or more and less than 0.040%, P: limited to 0.050% or less, N: limited to 0.0060% or less, O: limited to 0.0025% or less, and a remainder including an iron and impurities, and satisfies Ti/S≥6.0, in which, in a longitudinal section, a maximum diameter of Ti-based precipitates predicted by extreme value statistics under a condition that an inspection standard area is 100 mm | 2019-01-03 |
20190003000 | BOLT - A bolt is provided that has high strength and excellent hydrogen embrittlement resistance characteristics. A bolt according to an embodiment of the present invention consists of, in mass %, C: 0.32 to 0.39%, Si: 0.15% or less, Mn: 0.40 to 0.65%, P: 0.020% or less, S: 0.020% or less, Cr: 0.85 to 1.25%, Al: 0.005 to 0.060%, Ti: 0.010 to 0.050%, B: 0.0010 to 0.0030%, N: 0.0015 to 0.0080%, 0: 0.0015% or less, Mo: 0 to 0.05%, V: 0 to 0.05%, Cu: 0 to 0.50%, Ni: 0 to 0.30%, and Nb: 0 to 0.05%, with the balance being Fe and impurities. The bolt satisfies Formula (1) and Formula (2), and has a tensile strength of 1000 to 1300 MPa and satisfies Formula (3). | 2019-01-03 |
20190003001 | OIL-IMMERSION QUENCHING COOLING PRECURSOR AND OIL-IMMERSION QUENCHING COOLING METHOD - An oil-immersion quenching cooling precursor and an oil-immersion quenching cooling method includes an axle-type workpiece or a workpiece that has sections in an axle form. Several separation rings are arranged on the workpiece in the axial direction to separate the axle-type workpiece or the workpiece that has sections in an axle form into a plurality of sections before oil-immersion quenching cooling. In the method, there is a cutting procedure before a quenching cooling procedure. Several separation rings distributed in the axial direction are reserved outside a dimension required for the workpiece. sections before oil-immersion quenching cooling. In the method, there is a cutting procedure before a quenching cooling procedure. Several separation rings distributed in the axial direction are reserved outside a dimension required for the workpiece. | 2019-01-03 |
20190003002 | ULTRA-HIGH STRENGTH STEEL SHEET HAVING EXCELLENT PHOSPHATABILITY AND BENDABILITY AND METHOD FOR MANUFACTURING SAME - Provided is an ultra-high strength steel sheet having excellent phosphatability and bendability. The ultra-high strength steel sheet includes, by weight percentage (wt %), carbon (C): 0.08% to 0.2%, silicon (Si): 0.05% to 1.3%, manganese (Mn): 2.0% to 3.0%, phosphorus (P): 0.001% to 0.10%, sulfur (S): 0.010% or less, aluminum (Al): 0.01% to 0.1%, chromium (Cr): 0.3% to 1.2%, boron (B): 0.0010% to 0.0030%, titanium (Ti): 0.01% to 0.05%, nitrogen (N): 0.001% to 0.01%, a remainder of iron (Fe) and other inevitable impurities, satisfying: 3.4≤Ti/N≤10, 1.0≤Mn/(Si+Cr), and 0.7≤Mn*/(Si*+Cr*)≤Mn/(Si+Cr), where Ti, N, Mn, Si and Cr refer to a weight percentage (wt %), and Mn*, Si* and Cr* refer to an average of values obtained by GDS component analysis from a surface to a 0.1 μm position in a thickness direction. | 2019-01-03 |
20190003003 | Retention Of Mechanical Properties In Steel Alloys After Processing And In The Presence Of Stress Concentration Sites - This invention is related to retention of mechanical properties in high strength steel at reduced thicknesses and which mechanical property performance is also retained at relatively high strain rates. These new steels can offer advantages for a myriad of applications where reduced sheet thickness is desirable. In addition, the alloys herein are those that retain useful mechanical properties after introduction of a geometric discontinuity and an accompanying stress concentration. | 2019-01-03 |
20190003004 | VEHICLE PART HAVING HIGH STRENGTH AND EXCELLENT DURABILITY, AND MANUFACTURING METHOD THEREFOR - Provided are a part for vehicle having high strength and excellent durability, and a manufacturing method therefor. The part for vehicle comprises, by a weight ratio, a composition comprising 0.20-0.50% of C, 0.5% or less of Si, 1.0-2.0% of Mn, 0.01-0.1% of Al, 0.010% or less of P, 0.003% or less of S, 0.01-0.1% of Ti, 0.05-0.5% of Cr, 0.05-0.3% of Mo, 0.01% or less of N, and the remainder being Fe and other inevitable impurities, and the part for vehicle can have, by an area ratio, a microstructure comprising 90% or more of tempered martensite, 4% or less of retained austenite, and the remainder being one type or both of two types selected from among the ferrite and bainite structures. | 2019-01-03 |
20190003005 | Method for Producing a Steel Sheet Having Improved Strength, Ductility and Formability - A method for producing a steel sheet is provided. The steel sheet has a microstructure including, in area fraction, 20% to 50% intercritical ferrite, 10% to 20% retained austenite, 25% to 45% tempered martensite, 10% to 20% fresh martensite, and bainite. The sum of tempered martensite and bainite is between 30% and 60%. The method includes providing a cold-rolled steel sheet including, in weight percent, 0.18%≤C≤0.25%, 0.9%≤Si≤1.8%, 0.02%≤Al≤1.0%, with 1.00%≤Si+Al≤2.35%, 1.5%≤Mn 2.5%, 0.010%≤Nb≤0.035%, 0.10%≤Cr≤0.40%, and a remainder including Fe and unavoidable impurities. The method further includes annealing the steel sheet to obtain 50% to 80% austenite and 20% to 50% of ferrite, quenching the sheet at a cooling rate between 20° C./s and 50° C./s to a quenching temperature between Ms-50° C. and Ms-5° C., heating the sheet to a partitioning temperature between 375° C. and 450° C. and maintaining the sheet at the partitioning temperature for at least 50 s, then immediately cooling the sheet to room temperature. A steel sheet is also provided. | 2019-01-03 |
20190003006 | Mn-CONTAINING GALVANNEALED STEEL SHEET AND METHOD FOR PRODUCING THE SAME - High-strength galvannealed steel sheet including any of a) an oxide containing Fe and Mn, b) an oxide containing Fe and Mn and an Fe oxide, c) an oxide containing Fe and Mn and a Mn oxide, d) an oxide containing Fe and Mn, an Fe oxide, and a Mn oxide, and e) an Fe oxide and a Mn oxide is present in a zinc coated layer. The total amount of oxide is 0.01 to 0.100 g/m | 2019-01-03 |
20190003007 | Method for Producing a High Strength Steel Sheet Having Improved Strength and Formability, and Obtained High Strength Steel Sheet - A method for producing a steel sheet having a microstructure including 71% to 91% martensite and bainite, 9% to 13% retained austenite, and at most 20% ferrite is provided. The method includes providing a cold-rolled steel sheet including, in weight percent: 0.13%≤C≤0.22%, 1.2%≤Si≤2.3%, 0.02%≤Al≤1.0%, with 1.25%≤Si+Al≤2.35%, 2.4%≤Mn≤3%, Ti≤0.05%, Nb≤0.05% and a remainder of Fe and unavoidable impurities, annealing the steel sheet to obtain 80% to 100% austenite and 0% to 20% ferrite, quenching the steel sheet at a cooling rate between 20° C./s and 50° C./s to a quenching temperature between 240° C. and 310° C., heating the steel sheet to a partitioning temperature between 400° C. and 465° C. and maintaining the steel sheet at the partitioning temperature for 50 to 250 seconds, then immediately cooling the sheet to room temperature. Steel sheets are also provided. | 2019-01-03 |
20190003008 | Method For Producing a High Strength Steel Sheet Having Improved Ductility and Formability, and Obtained Steel Sheet - A method for producing a steel sheet is provided. The method includes providing a cold-rolled steel sheet including in weight %: 0.15%≤C≤0.23%, 1.4%≤Mn≤2.6%, 0.6%≤Si≤1.5%, 0.02%≤Al≤1.0%, with 1.0%≤Si+Al≤2.0%, 0≤Nb≤0.035%, 0≤Mo≤0.3%, 0≤Cr≤0.3%, and a remainder of Fe and unavoidable impurities, annealing the steel sheet at an annealing temperature between Ac1 and Ac3 to obtaining at least 40% austenite and at least 40% intercritical ferrite, quenching the sheet from at least 600° C. at a cooling rate of at least 20° C./s to a quenching temperature between 180° C. and 260° C., heating the sheet to a partitioning temperature between 375° C. and 470° C. and maintaining the sheet at this partitioning temperature for a partitioning time Pt between 25 s and 440 s, then cooling the sheet to room temperature. A steel sheet is also provided. | 2019-01-03 |
20190003009 | HIGH-STRENGTH STEEL SHEET AND HIGH-STRENGTH GALVANIZED STEEL SHEET - A high-strength steel sheet includes: a specific chemical composition; and a microstructure represented by, in a ⅛ thickness to ⅜ thickness range with ¼ thickness of a sheet thickness from a surface being a center, in volume fraction, ferrite: 85% or less, bainite: 3% or more and 95% or less, tempered martensite: 1% or more and 80% or less, retained austenite: 1% or more and 25% or less, pearlite and coarse cementite: 5% or less in total, and fresh martensite: 5% or less, in which the solid-solution carbon content in the retained austenite is 0.70 to 1.30 mass %, and to all grain boundaries of retained austenite grains having an aspect ratio of 2.50 or less and a circle-equivalent diameter of 0.80 μm or more, the proportion of interfaces with the tempered martensite or the fresh martensite is 75% or less. | 2019-01-03 |
20190003010 | RECOVERY OF LITHIUM FROM SILICATE MINERALS - A process and system are disclosed for recovering lithium from a lithium-containing silicate mineral. The process and system comprise mixing the silicate mineral with nitric acid. The process and system also comprise subjecting the mixture to a leaching process having conditions such that lithium values in the silicate mineral are leached from the silicate mineral as lithium nitrate. The nitric acid can be in aqueous, gaseous or precursor gaseous form. | 2019-01-03 |
20190003011 | PROCESSES FOR REFINING NIOBIUM-BASED FERROALLOYS - Refined niobium-based ferroalloys are provided by removing lead and other impurities therefrom by a process comprising charging niobium ore concentrate and/or niobium oxide or a mixture of niobium oxides to a metallothermic reaction chamber, admixing the ore concentrate and/or niobium oxide with a reducing agent, initiating a metallothermic reaction, under reduced pressure; and allowing the reaction product to solidify and cool; crushing the reaction product or crushing the niobium-based ferroalloy ore concentrate previously reduced in open air, and charging the crushed product to a melting crucible within a vacuum induction melting furnace, lowering the pressure within the furnace to below 1 mbar, and melting the crushed product while vaporizing the impurities contained therein. | 2019-01-03 |
20190003012 | PROCESSES FOR REFINING NIOBIUM-BASED FERROALLOYS - Refined niobium-based ferroalloys are provided by removing lead and other impurities therefrom by a process comprising charging niobium ore concentrate and/or niobium oxide or a mixture of niobium oxides to a metallothermic reaction chamber, admixing the ore concentrate and/or niobium oxide with a reducing agent, initiating a metallothermic reaction, under reduced pressure; and allowing the reaction product to solidify and cool; crushing the reaction product or crushing the niobium-based ferroalloy previously reduced in open air, and charging the crushed product to a melting crucible within a vacuum induction melting furnace, lowering the pressure within the furnace to below 1 mbar, and melting the crushed product while vaporizing the impurities contained therein. | 2019-01-03 |
20190003013 | PROCESSES FOR PRODUCING LOW NITROGEN METALLIC CHROMIUM AND CHROMIUM-CONTAINING ALLOYS AND THE RESULTING PRODUCTS - Processes for producing low-nitrogen metallic chromium or chromium-containing alloys, which prevent the nitrogen in the surrounding atmosphere from being carried into the melt and being absorbed by the metallic chromium or chromium-containing alloy during the metallothermic reaction, include vacuum-degassing a thermite mixture comprising metal compounds and metallic reducing powders contained within a vacuum vessel, igniting the thermite mixture to effect reduction of the metal compounds within the vessel under reduced pressure i.e., below 1 bar, and conducting the entire reduction reaction in said vessel under reduced pressure, including solidification and cooling, to produce a final product with a nitrogen content below 10 ppm. The final products obtained, in addition to low-nitrogen metallic chromium in combination with other elements, can be used as raw materials in the manufacture of superalloys, stainless steel and other specialty steels whose final content of nitrogen is below 10 ppm. | 2019-01-03 |
20190003014 | HARD METAL MATERIALS - A hard metal material and a method of manufacturing a component of the hard metal material are disclosed. The hard metal material comprises 5-50 volume % particles of a refractory material dispersed in a host metal. The method comprises forming a slurry of 5-50 volume % particles of the refractory material dispersed in a liquid host metal in an liquid atmosphere and pouring the slurry into a mould and forming a casting of the component. | 2019-01-03 |