| Entries |
| Document | Title | Date |
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| 20110123993 | EXPRESSION VECTOR FOR MASS PRODUCTION OF FOREIGN GENE-DERIVED PROTEIN USING ANIMAL CELL AND USE THEREOF - The present inventors successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired drug resistance gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal. | 05-26-2011 |
| 20110123994 | SCREENING FOR COMPOUNDS HAVING IMMUNOSUPPRESSANT ACTIVITY BY TESTING IMPACT ON LEUKOCYTE-SPECIFIC CALCIUM FLUXES - The invention relates to an assay for the identification of a compound having immunosuppressant activity, wherein a candidate compound is analyzed whether it blocks the Ca | 05-26-2011 |
| 20110129819 | DIAGNOSIS OF HEREDITARY SPASTIC PARAPLEGIAS (HSP) BY INDENTIFICATION OF A MUTATION IN THE KIAA1840 GENE OR PROTEIN - The invention relates to an ex vivo method of diagnosing or predicting an hereditary spastic paraplegias (HSP), in a subject, which method comprises detecting a mutation in the KIAA1840 gene or protein (spatacsin), wherein said mutation is indicative of. an hereditary spastic paraplegias (HSP). | 06-02-2011 |
| 20110129820 | HUMAN DIABETES SUSCEPTIBILITY TNFRSF10B GENE - The present invention relates to a diagnostic method of determining whether a subject is at risk of developing type 2 diabetes, which method comprises detecting the presence of an alteration in the TNFRSF10B gene locus in a biological sample of said subject. | 06-02-2011 |
| 20110129821 | METHODS FOR CYCLIC NUCLEOTIDE DETERMINATION - The present invention relates in general to cellular analysis tools and more particularly to methods for detecting or determining cyclic nucleotide concentrations in samples. Samples containing cyclic nucleotides may be contacted with a cyclic nucleotide-dependent protein kinase and a detection system which includes a substrate for the cyclic nucleotide-dependent protein kinase. The activities in cyclic nucleotide related pathways may be measured using the detection system. | 06-02-2011 |
| 20110129822 | MULTI DRUG RESPONSE MARKERS FOR BREAST CANCER CELLS - The present invention provides methods for preparing a gene expression profile of a breast cancer cell, tumor, or cell line, where the gene expression profile may be evaluated for one or more gene expression signatures indicative of multidrug resistance. The signature may be indicative of resistance to one or more chemotherapeutic agents selected from a Taxol (e.g., Docetaxel or Paclitaxel), an antibiotic (e.g., Doxorubicin or Epirubicin), an antimetabolite (e.g., Fluorouracil and/or Gemcitabine), and an alkylating agent (e.g., Cyclophosphamide). Generally, the gene expression profile contains the level of expression for a plurality of genes listed in FIGS. | 06-02-2011 |
| 20110129823 | Enzymatic Detection Techniques - A diagnostic test kit for detecting the presence or quantity of an enzyme or enzyme inhibitor is provided. The diagnostic kit utilizes reactive complexes to facilitate the detection of the enzyme or enzyme inhibitor. The reactive complexes include a substrate joined (e.g., covalently bonded, physically adsorbed, etc.) to a reporter and specific binding member. In one embodiment, for example, a peptide, protein, or glycoprotein substrate is joined to a reporter (e.g., dyed latex particle) and specific binding member (e.g., biotinylated compound). In this embodiment, the substrate provides a cleavage target for a proteolytic enzyme. Specifically, upon contacting the reactive complexes, the proteolytic enzyme cleaves the substrate and releases the reporter and/or specific binding member. The signal exhibited by the released reporters may then be used to indicate the presence or quantity of an enzyme or enzyme inhibitor within the test sample. | 06-02-2011 |
| 20110136106 | METHODS FOR DETECTING RICIN AND RELATED COMPOUNDS AND USES THEREOF - The present invention is directed to methods for detecting the presence of ricin and compounds that catalyze the release of adenine from nucleic acids or other biological materials and for screening for inhibitors of such compounds. | 06-09-2011 |
| 20110136107 | Diagnostics and Therapeutics for Diseases Associated with G-Protein Coupled Receptor AdipoR2 (AdipoR2) - The invention provides human AdipoR2 which is associated with the cardiovascular diseases, dermatological diseases, gastroenterological diseases, cancer, hematological diseases, respiratory diseases, inflammation, neurological diseases, urological diseases. The invention also provides assays for the identification of compounds useful in the treatment or prevention of cardiovascular diseases, dermatological diseases, gastroenterological diseases, cancer, hematological diseases, respiratory diseases, inflammation, neurological diseases, urological diseases. The invention also features compounds which bind to and/or activate or inhibit the activity of AdipoR2 as well as pharmaceutical compositions comprising such compounds. | 06-09-2011 |
| 20110136108 | XBP1(S) Protein Acting as an Adipocyte Differentiation Marker Having a Facility to Regulate Differentiation into Adipocytes, and an Application Therefor - Disclosed are a protein marker, indicative of an increase in differentiation into adipocytes, comprising an XBP1(S) having an amino acid sequence of SEQ ID NO. 1, 2 or 3, and the uses thereof in developing a promoter of adipocyte differentiation and a method for promoting adipocyte differentiation, a repressor of adipocyte differentiation and a method for repressing adipocyte differentiation, an agent and a method for screening a repressor of adipocyte differentiation, and a method for reducing rosiglitazone's side effect of causing obesity. Also, provided is a protein marker, indicative of an increase in differentiation into adipocytes, comprising an XBP1(U) having an amino acid sequence of SEQ ID NO. 4, 5 or 6. When targeting the XBP1(S) gene or protein, a compound capable of blocking or restraining differentiation into adipocytes can be used to develop an agent for the prevention and treatment of obesity. | 06-09-2011 |
| 20110136109 | System And Method For Cycling Liquid Samples Through A Series Of Temperature Excursions - A system and method for cycling liquid samples through a series of temperature excursions are disclosed. Provided are open-top reaction vessels for containing the samples, which may be enclosed by one or more covers. A temperature-controlled block for generating or adsorbing heat is coupled thermally to the reaction vessels. A detection arrangement is disposed in an emission beam path to detect radiation emitted from the samples through the covers. A heating arrangement for generating heat includes a heating element that is both disposed between the reaction vessels and the detection arrangement and coupled thermally to the covers. The heating element includes an optically transparent substrate provided with one or more opaque heating lines, the heating lines being disposed in the emission beam path in a manner to obtain a predetermined minimum optical transmission of the heating element. A controller, set up to control cycling of the samples, is provided. | 06-09-2011 |
| 20110136110 | METHOD FOR THE PRODUCTION OF MEDIUM-CHAIN SOPHOROLIPIDS - The MFE2 gene of a microorganism capable of producing glycolipids, such as, but not limited to | 06-09-2011 |
| 20110136111 | NUCLEIC ACID LIGANDS AGAINST INFECTIOUS PRIONS - The invention generally relates to nucleic acid ligands that specifically bind to infectious prions, and methods of diagnosing a transmissible spongiform encephalopathy disease in a subject. In certain embodiments, the invention provides an isolated nucleic acid ligand that binds to an infectious prion. In other embodiments, the invention provides a method for diagnosing a transmissible spongiform encephalopathy disease in a subject including obtaining a tissue or body fluid sample from a subject, contacting the tissue or body fluid with a nucleic acid ligand that binds to an infectious prion, thereby detecting the infectious prion in the sample, and diagnosing the transmissible spongiform encephalopathy disease based on results of the contacting step. | 06-09-2011 |
| 20110136112 | IDENTIFICATION OF BITTER LIGANDS THAT SPECIFICALLY ACTIVATE HUMAN T2R RECEPTORS AND RELATED ASSAYS FOR IDENTIFYING HUMAN BITTER TASTE MODULATORS - The present invention relates to the discovery that specific human taste receptors in the T2R taste receptor family respond to particular bitter compounds. Also, the invention relates to the discovery of specific hT2R9 alleles and their disparate activity in functional assays with the same biter ligands. The invention further relates to the use of these T2R receptors in assays for identifying ligands that modulate the activation of these taste receptors by specific bitter ligands and related compounds. These compounds may be used as additives and/or removed from foods, beverages, cosmetics and medicinals in order to modify (block) T2R-associated bitter taste. Also T2R ligands may be used as therapeutics to treat and modulate T2R associated gastrointestinal and metabolic functions as well as treat gastrointestinal and metabolic diseases such as eating disorders, food sensing, food absorption, obesity, diabetes, Crohn's diseae, celiac disease, et al. | 06-09-2011 |
| 20110136113 | MARKER FOR DETECTING IL-17-PRODUCING HELPER T CELL, AND METHOD FOR DETECTING IL-17-PRODUCING HELPER T CELL - Disclosed is at least one polynucleotide marker or protein marker which enables the specific detection of an IL-17-producing helper T cell (a Th17 cells). Also disclosed is a method for detecting a Th17 cell, which is characterized by comprising detecting the occurrence of the above-mentioned at least one marker. | 06-09-2011 |
| 20110143338 | NUCLEIC ACID ENZYMES AND COMPLEXES AND METHODS FOR THEIR USE - The present invention relates to methods that utilise multi-component nucleic acid complex (MNA complex) cascades. The MNA complexes may have cleavage or ligase activity. Further, the invention provides cascades which may include one or more DNAzymes. The invention also provides methods which use these cascades for the identification, detection and quantification of targets. | 06-16-2011 |
| 20110143339 | Device, System and Method for Processing a Sample - A device for processing a sample comprises a blister defined by first and second walls. The first wall is flexible allowing the blister to be divided into one or more sealed regions by an external pressure applied to a portion of the first wall. The external pressure is applied in the form of a 2-dimensional shape to form a sealed region having that shape. | 06-16-2011 |
| 20110143340 | NON-INVASIVE ISOLATION OF FETAL NUCLEIC ACID - The present invention provides compositions comprising a solution of a compound useful for lysing biological cells and methods for using the compositions to lyse biological cells and to isolate nucleic acid. | 06-16-2011 |
| 20110143341 | Microsatellite Markers of Schizophrenia - The invention includes methods of determining if a subject is at risk for developing schizophrenia (SZ). | 06-16-2011 |
| 20110151446 | CELL TREATMENT SOLUTION AND METHOD OF PREPARING STAINED CELL SUSPENSION FOR A MEASUREMENT OF NUCLEAR DNA BY FLOW CYTOMETRY - A cell treatment solution and a method that is used for preparing a stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry. The cell treatment solution may include a surfactant, RNase, and a fluorescent dye. The surfactant may include, for example, a non-ionic surfactant, a zwitterionic surfactant, an anionic surfactant, and/or a cationic surfactant. In one method of the invention, stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry is prepared. The method may include adding a tissue sample to a cell treatment solution including a surfactant, RNase, and fluorescent dye, disaggregating the tissue sample, and filtering the disaggregated tissue sample. Another method of the invention includes disaggregating a tissue sample, preparing cell suspension by filtering the disaggregated tissue sample, and adding a cell treatment solution including a surfactant, RNase, and fluorescent dye. | 06-23-2011 |
| 20110151447 | METHOD TO PRODUCE INDUCED PLURIPOTENT STEM (IPS) CELLS FROM NON-EMBRYONIC HUMAN CELLS - The invention provides methods for generating induced pluripotent stem (iPS) cells from normal and mutant adult cells, as well as the iPS cells so generated from such methods. In some aspects, iPS cells are generated by ectopically expressing SOX2 and OCT4 nucleic acids in such adult cells. Other nucleic acids such as but not limited to MYC may also be ectopically expressed in such adult cells in the methods described herein. | 06-23-2011 |
| 20110151448 | Methods and Systems for High Homologous Recombination ("HR") Targeting Efficiency - Disclosed are vectors, kits and methods useful in the construction of recombinant cells and DNAs via enhanced efficiency homologous recombination. The vectors are targeting vectors that contain a gene-of-interest spliced between two ends that are homologous to a genome target site. The ends of the vector may be protected from exonuclease attack by deploying a cap, such as a hair pin structure. The vector is linked to a nuclear localization signal sequence, and preferably, a bait peptide that binds to RAD51, to facilitate homologous recombination. The vector may be deployed in myriad genetic transformation applications, such as site-directed mutagenesis, gene therapy, and the like. | 06-23-2011 |
| 20110151449 | SHORT CYCLE METHODS FOR SEQUENCING POLYNUCLEOTIDES - The invention provides methods for sequencing a polynucleotide comprising stopping an extension cycle in a sequence by synthesis reaction before the reaction has run to near or full completion. | 06-23-2011 |
| 20110151450 | High Fidelity Restriction Endonucleases - Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer. | 06-23-2011 |
| 20110159485 | Lysis Buffers for Extracting Nucleic Acids - The present teachings relate to the extraction of nucleic acid from solid materials. Provided are useful compositions, methods and kits for obtaining nucleic acids from a solid biological sample or an adhesive material having a biological material adherent or embedded within the adhesive substrate. The extracted nucleic acid can be used in downstream applications such as genotyping, detection, quantification, and identification of the source of the biological material. | 06-30-2011 |
| 20110159486 | CELL CYCLE SWITCH 52(CCS52) AND METHODS FOR INCREASING YIELD - Methods and compositions for modulating plant yield are provided. Methods include employing cell cycle switch 52 (ccs52). The ccs52 sequences are used in a variety of methods including modulating plant biomass, growth, or both. Transformed plants, plant cell, tissues, seed, and expression vectors are also provided. | 06-30-2011 |
| 20110159487 | Identification of a Gene Expression Profile that Differentiates Ischemic and Nonischemic Cardiomyopathy - A method of preparing a gene expression prediction profile for distinguishing ischemic and nonischemic cardiomyopathy comprises the steps of obtaining clinical specimens from patients suffering from ischemic or nonischemic cardiomyopathy, isolating nucleic acid sequences from at least a plurality of said specimens, obtaining a gene expression level corresponding to each individual of said nucleic acid sequence by a gene expression profiling method, identifying genes having differences in gene expression by comparing the gene expression level of an ischemic specimen with the gene expression level of a nonischemic specimen, and identifying a gene expression prediction profile comprises genes identified as having differences in gene expression so that said prediction profile distinguishes ischemic and nonischemic cardiomyopathy. | 06-30-2011 |
| 20110165559 | Lateral flow based methods and assays for rapid and inexpensive diagnostic tests - The invention provides reagents and methods for lateral flow assays and quantitative capture or determination of components, including cells, in a sample. In one aspect, reagents and methods for diagnostic assay are provided. In one embodiment an assay for determining T cell numbers, particularly a CD2+ CD4+ T cell assay is provided. A manufacturing method for producing rapid diagnostic assays in a decentralized manner is also described. The method generates net economic advantages over conventional diagnostic manufacturing practices. | 07-07-2011 |
| 20110165560 | METHODS AND COMPOSITIONS FOR SCREENING FOR INDIVIDUALS AT RISK FOR SUCCINATE DEHYDROGENASE-RELATED DISEASE CONDITIONS - The present invention provides methods, compositions, and kits associated with succinate dehydrogenase-related disease conditions. In one aspect of the present invention, a method for screening a test subject to determine risk for developing a succinate dehydrogenase-related disease condition is provided. Such a method can include obtaining a biological sample from the test subject and identifying a mutation in gene hSDH5 from the biological sample of the test subject, wherein the mutation effectuates a decreased level of succinate dehydrogenase flavination in the test subject as compared to a level of succinate dehydrogenase flavination in a normal subject. In one aspect, the mutation is at hSDH5 Gly78 of gene hSDH5. In another aspect, the mutation is equivalent to an hSDH5 Gly78Arg substitution of gene hSDH5. | 07-07-2011 |
| 20110165561 | DIRECT AND CONTINUOUS ROOT ALONE OR ROOT/SHOOT PRODUCTION FROM TRANSGENIC EVENTS DERIVED FROM GREEN REGENERATIVE TISSUES AND ITS APPLICATIONS - The present invention provides assays and methods for efficiently testing a polynucleotide of interest for a phenotype in a root. In some embodiments, the assays and methods include regenerating green tissue that is transgenic for at least one polynucleotide of interest into one or more transgenic plantlets that have at least one transgenic root. Further provided are methods of making a root assay by contacting green tissue with a first rooting medium to produce a plantlet and a plurality of roots. Additionally provided are methods of assaying for insecticidal activity on a live root. Accordingly provided herein is a substantially contamination-free, root bioassay. Further provided are methods of identifying a promoter having activity in a root. | 07-07-2011 |
| 20110165562 | METHOD FOR SEPARATING AN ANALYTE FROM A SAMPLE - An analyte is separated from a fluid sample by introducing the sample into a cartridge having a sample port and a first flow path extending from the sample port. The first flow path includes an extraction chamber containing a solid support for capturing the analyte from the sample. The cartridge has a second flow path for eluting the captured analyte from the extraction chamber, the second flow diverging from the first flow path after passing through the extraction chamber. The sample is forced to flow through the extraction chamber and into a waste chamber, thereby capturing the analyte with the solid support as the sample flows through the extraction chamber. The captured analyte is then eluted from the extraction chamber by forcing an elution fluid to flow through the extraction chamber and along the second flow path. | 07-07-2011 |
| 20110165563 | DESIGN, SYNTHESIS AND USE OF SYNTHETIC NUCLEOTIDES COMPRISING CHARGE MASS TAGS - Embodiments of the present disclosure relate generally to reporter compositions which are synthetic nucleotides that comprise nucleotides with a high charge mass moiety attached thereto via a linker molecule. The linker molecules can vary in length in part to enable the high charge mass moiety to extend out from a DNA polymerase complex so that polymerization may not be influenced. | 07-07-2011 |
| 20110171630 | METHODS AND COMPOSITIONS FOR CORRELATING GENETIC MARKERS WITH CARDIOVASCULAR DISEASE - The present invention provides methods of identifying a subject having an increased or decreased risk of developing cardiovascular disease, comprising: a) correlating the presence of one or more genetic markers in chromosome 3q13.31 with an increased or decreased risk of developing cardiovascular disease; and b) detecting the one or more genetic markers of step (a) in the subject, thereby identifying the subject as having an increased or decreased risk of developing cardiovascular disease. Also provided are methods of identifying subjects with cardiovascular disease as having a good or poor prognosis, as well as methods of identifying effective treatment regimens for cardiovascular disease, based on correlation with genetic markers in chromosome 3q13.31. | 07-14-2011 |
| 20110171631 | METHODS OF DIAGNOSING ENDOMETRIOSIS - The present invention provides biomarkers for the diagnosis and prognosis of endometriosis. Generally, the methods of this invention find use in diagnosing or for providing a prognosis for endometriosis by detecting the expression levels of biomarkers, which are differentially expressed (up- or down-regulated) in endometrial cells from a patient with endometriosis. Similarly, these markers can be used to diagnose reduced fertility in a patient with endometriosis or to provide a prognosis for a fertility trial in a patient suffering from endometriosis. The present invention also provides methods of identifying a compound for treating or preventing endometriosis. Finally, the present invention provides kits for the diagnosis or prognosis of endometriosis. | 07-14-2011 |
| 20110171632 | LOSS OF HETEROZYGOSITY OF THE DNA MARKERS IN THE 12Q22-23 REGION - A method of detecting DNA markers in the 12q22-23 region. The method comprises providing a sample containing acellular DNA from a subject and detecting one or more DNA markers in the 12q22-23 region in the sample. Also disclosed are methods of diagnosing and monitoring cancer; methods of determining the efficacy of a therapy, and the probabilities of survival and responsiveness to a therapy; and packaged products for using these methods. | 07-14-2011 |
| 20110171633 | METHOD TO USE GENE EXPRESSION TO DETERMINE LIKELIHOOD OF CLINICAL OUTCOME OF RENAL CANCER - The present disclosure provides gene and gene sets, the expression of which is important in the classification and/or prognosis of cancer, in particular of renal cell carcinoma. | 07-14-2011 |
| 20110171634 | METHODS AND DEVICES FOR SINGLE-MOLECULE WHOLE GENOME ANALYSIS - Provided are methods and devices for single-molecule genomic analysis. In one embodiment, the methods entail processing a double-stranded nucleic acid and characterizing said nucleic acid. These methods are useful in, e.g., determining structural variations and copy number variations between individuals. | 07-14-2011 |
| 20110171635 | NUCLEIC ACID ENZYME BIOSENSORS FOR IONS - Disclosed are compositions and methods for the sensitive and selective detection of ions using nucleic acid enzymes. | 07-14-2011 |
| 20110171636 | MONO- AND MULTI-ELEMENT CODED LIBS ASSAYS AND METHODS - Methods for tagging an object with an element-coded particle and identifying the object based on the element code are described. LIBS analysis can be used with the methods to provide a high resolution system for identifying and quantifying objects with great specificity. Objects can include biological and chemical molecules. | 07-14-2011 |
| 20110177496 | FIELD-SWITCH SEQUENCING - The present invention provides novel compositions, methods and apparatus for DNA sequencing that can be performed, e.g., in a two-electrode chamber. The present invention also provides a method for sequencing a nucleic acid comprising immobilizing a plurality of complexes comprising a target nucleic acid, a primer nucleic acid, and a polymerase onto a surface, contacting the surface with a plurality of charged particles comprising a nucleotide phosphate by applying an electric field, reversing the electric field to transport unbound charged particles away from the surface, and detecting the incorporation of a nucleotide phosphate into a single molecule of the primer nucleic acid. | 07-21-2011 |
| 20110177497 | Thermus Thermophilus Nucleic Acid Polymerases - The invention provides novel nucleic acid polymerases from strains GK24 and RQ-1 of | 07-21-2011 |
| 20110177498 | BASE-DETECTING PORE - The invention relates to a mutant α-hemolysin (α-HL) pore which is useful for detecting one or more nucleotides by stochastic sensing. The pore is particularly useful for sequencing DNA or RNA. A molecular adaptor that allows detection of the nucleotide(s) is covalently attached to the pore. The pore is specifically modified to facilitate positioning of the adaptor and may be modified to facilitate covalent attachment. | 07-21-2011 |
| 20110177499 | Methods and Systems for Molecular Fingerprinting - This invention relates in general to a method for molecular fingerprinting. The method can be used for forensic identification (e.g. DNA fingerprinting, especially by VNTR), bacterial typing, and human/animal pathogen diagnosis. More particularly, molecules such as polynucleotides (e.g. DNA) can be assessed or sorted by size in a microfabricated device that analyzes the polynucleotides according to restriction fragment length polymorphism. In a microfabricated device according to the invention, DNA fragments or other molecules can be rapidly and accurately typed using relatively small samples, by measuring for example the signal of an optically-detectable (e.g., fluorescent) reporter associated with the polynucleotide fragments. | 07-21-2011 |
| 20110183318 | METHOD FOR SCREENING OF SUBSTANCE EFFECTIVE ON DISEASE USING GPR120 AND PHOSPHOLIPASE - The present invention provides a screening method for determining whether a substance of interest is a substance which alters GPR120 mediated cell stimulating activities, comprising using a substance of interest, a biomembrane containing GPR120, or cells containing said biomembrane, and phospholipase or salts thereof. According to a screening method of the present invention, the method can screen substances such as CCK and GLP-1 which are involved in the secretion of hormones in gastrointestinal tracts. | 07-28-2011 |
| 20110183319 | Compositions and methods for therapeutic delivery with microorganisms - Certain embodiments disclosed relate to compositions, including therapeutic compositions, methods, devices, and systems that include modified microorganisms including at least one genetic element encoding at least one therapeutic agent or environmental treatment agent. | 07-28-2011 |
| 20110183320 | CLASSIFICATION OF NUCLEIC ACID TEMPLATES - Methods, compositions, and systems are provided for characterization of modified nucleic acids. In certain preferred embodiments, single molecule sequencing methods are provided for identification of modified nucleotides within nucleic acid sequences. Modifications detectable by the methods provided herein include chemically modified bases, enzymatically modified bases, abasic sites, non-natural bases, secondary structures, and agents bound to a template nucleic acid. | 07-28-2011 |
| 20110183321 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 07-28-2011 |
| 20110183322 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 07-28-2011 |
| 20110183323 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 07-28-2011 |
| 20110183324 | TUMOR SUPPRESSOR GENE P33ING2 - The invention provides isolated nucleic acid and amino acid sequences of novel human tumor suppressors, antibodies to such tumor suppressors, methods of detecting such nucleic acids and proteins, methods of screening for modulators of tumor suppressors, and methods of diagnosing and treating tumors with such nucleic acids and proteins. | 07-28-2011 |
| 20110183325 | METHOD AND APPARATUS FOR DISRUPTING CELLS AND PURIFYING NUCLEIC ACID USING A SINGLE CHIP - Provided herein is a method and apparatus for disrupting cells and purifying nucleic acids in a single chip. The method comprises irradiating a chip with a laser beam, wherein the chip comprises a solid support on which a cell lysis enhancing metal oxide layer, and a cell binding metal oxide layer have been deposited. | 07-28-2011 |
| 20110189657 | Device and a Method for the Detection and Amplification of a Signal - The invention relates to a device and a method for the detection and amplification of a primary signal, utilizing an intracellular communication system, and the use thereof for the detection of substances such as phosphorus, sulfur, nitrogen, hormones, metabolic intermediates, fermentation products, and so forth. The device according to the invention for the detection and amplification of a primary signal contains cells of a first type for which a gene, which is responsible for the synthesis of a signal molecule, is under the control of a promoter which is regulated by the primary signal, and cells of a second type for which a specific gene is under the control of a promoter which is regulated by the separated signal molecule, in such a way that the secretion of the signal molecule is induced by a primary signal taken up by a cell of the first type, and the primary signal is amplified by the cells of the second type by the expression of the specific gene under the control of the signal molecule. | 08-04-2011 |
| 20110189658 | CELL, METHOD AND KIT FOR CONDUCTING AN ASSAY FOR NEUTRALIZING ANTIBODIES - The present invention provides a cell for use in a one-step cell-based assay for an extracellular ligand (e.g., I FNα) that initiates a ligand-specific signal at the nucleus of the cell and for neutralizing antibodies against the extracellular ligand. The cell-based one-step assay allows both the extracellular ligand concentration and the neutralizing antibody titer to be quantified in a single sample (e.g., serum) without the need for sample dilution and addition of exogenous extracellular ligand. | 08-04-2011 |
| 20110189659 | Generation of modified polymerases for improved accuracy in single molecule sequencing - Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions and/or heterologous or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include reduced reaction rates at one or more steps of the polymerase kinetic cycle, increased closed polymerase/DNA complex stability, enhanced metal ion coordination, reduced exonuclease activity, decreased branching fractions, and the like. Polymerases that exhibit branching fractions that are less than the branching fractions of the polymerases from which they were derived, or branching fractions that are less than about 25% for a phosphate-labeled nucleotide analog, are also provided. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template. | 08-04-2011 |
| 20110189660 | BIOCHIP, SAMPLE REACTION APPARATUS, AND SAMPLE REACTION METHOD - A biochip includes: a first chamber; a second chamber filled with a wax, a melting point of the wax being 25° C. or more and 63° C. or less; an injection path between the first chamber and the second chamber; and a sub-chamber that includes a wall at least partially made of the wax, the sub-chamber is formed inside the second chamber and communicates with the first chamber via the injection path. | 08-04-2011 |
| 20110189661 | GRAVITY-ASSISTED MIXING METHODS - A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle. | 08-04-2011 |
| 20110189662 | METHOD FOR MEASURING SURVIVIN mRNA - Disclosed is a method of amplifying and detecting mRNA of survivin gene in an RNA amplification process comprising: a step for forming a double-stranded DNA containing a promoter sequence by use of a combination of oligonucleotides consisting of a first primer having a sequence homologous with a portion of survivin mRNA and a second primer having a complementary sequence, wherein the promoter sequence is added to the 5′-end of either the first primer or the second primer with a reverse transcriptase, forming an RNA transcription product by use of an RNA polymerase with using the double-stranded DNA as template, and forming the double-stranded DNA by use of a reverse transcriptase by continuing to use the RNA transcription product as a template of DNA synthesis, measuring an amount of amplified RNA produced over time with an oligonucleotide probe designed so that signal properties change with the formation of a complementary double strand with the amplified RNA. | 08-04-2011 |
| 20110195395 | RINSE COMPOSITION FOR THE STABILIZATION OF DIAGNOSTIC BIOMARKERS - Rinse composition for the stabilization of diagnostic biomarkers. In at least one embodiment of a rinse useful to stabilize a diagnostic marker, the rinse comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker in saliva, and a liquid. | 08-11-2011 |
| 20110195396 | CHEWABLE COMPOSITIONS FOR THE STABILIZATION OF DIAGNOSTIC BIOMARKERS - Chewable compositions for the stabilization of diagnostic biomarkers. In at least one embodiment of a chewing gum composition useful to stabilize a diagnostic marker, the a chewing gum composition comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker in saliva, and a liquid. | 08-11-2011 |
| 20110195397 | DRINK MIXTURE FOR THE STABILIZATION OF DIAGNOSTIC BIOMARKERS - Drink mixture for the stabilization of diagnostic biomarkers. In at least one embodiment of an ingestible beverage useful to stabilize a diagnostic marker, the rinse comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker in saliva, and a liquid. | 08-11-2011 |
| 20110195398 | SYSTEMS AND METHODS FOR DETECTING INSULIN RESISTANCE BIOMARKERS - Systems and methods for detecting insulin resistance biomarkers. At least one embodiment of a system of the present disclosure comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker for insulin resistance or glucose intolerance in a body fluid comprising the diagnostic marker, and a detection agent capable of detecting the diagnostic marker. | 08-11-2011 |
| 20110195399 | SYSTEMS AND METHODS FOR THE INHIBITION OF GALACTOSE OXIDASE - Systems and methods for the inhibition of galactose oxidase. According to least one embodiment of a stabilizing system of the present disclosure, the system comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker by galactose oxidase in a body fluid including the diagnostic marker. The stabilizing system also includes a detection agent capable of detecting the diagnostic marker. | 08-11-2011 |
| 20110195400 | SYSTEMS AND METHODS FOR DIAGNOSING CANCER - Systems and methods for diagnosing cancer. According to at least one embodiment of a stabilizing system of the present disclosure, the system comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker for cancer in a body fluid comprising the diagnostic marker and a detection agent capable of detecting the diagnostic marker. | 08-11-2011 |
| 20110195401 | SYSTEMS AND METHODS FOR DETERMINING FOOD ALLERGIES - Systems and methods for determining food allergies. In at least one embodiment of a system of the present disclosure, the system comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker for a food allergy in a body fluid comprising the diagnostic marker and a detection agent capable of detecting the diagnostic marker. | 08-11-2011 |
| 20110195402 | SYSTEMS AND METHODS FOR DETECTING DRUG USE - Systems and methods for detecting drug use. In at least one embodiment of a system of the present disclosure, the system comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker indicative of drug use in a body fluid, comprising the diagnostic marker, and a detection agent capable of detecting the diagnostic marker. | 08-11-2011 |
| 20110195403 | SYSTEMS AND METHODS FOR FERTILITY ANALYSIS - Systems and methods for fertility analysis. In at least one embodiment of a system of the present disclosure, the system comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker for fertility in a body fluid comprising the diagnostic marker, and a detection agent capable of detecting the diagnostic agent. In an exemplary embodiment, the stabilizing agent may be selected from the group consisting of a protease inhibitor, a DNase inhibitor, and a RNase inhibitor. | 08-11-2011 |
| 20110195404 | SYSTEMS AND METHODS FOR DETERMINING CALCIUM LEVELS - Systems and methods for determining calcium levels. In at least one embodiment of a system of the present disclosure, the system comprises a stabilizing agent useful to completely or substantially prevent degradation or inactivation of a diagnostic marker for circulating calcium in a body fluid comprising the diagnostic marker, and a detection agent capable of detecting the diagnostic agent. | 08-11-2011 |
| 20110195405 | COMPOSITIONS, METHODS, AND KITS FOR ANALYZING DNA METHYLATION - Compositions, methods, and kits for reducing strand amplification bias using bisulfite treated gDNA are provided. Methods for detecting and for quantitating the amplified bisulfite treated gDNA and inferring the presence, absence, and/or degree of methylation of target cytosine(s) in the gDNA are also provided. Such methods typically employ tailed first primer pairs, which can, but need not comprise nucleotide analogs, and optionally second primer pairs. | 08-11-2011 |
| 20110195406 | INTERMITTENT DETECTION DURING ANALYTICAL REACTIONS - Methods, devices, and systems for performing intermittent detection during analytical reactions are provided. Such methods facilitate collection of reaction data from disparate reaction times. Further, such methods are useful for reducing photo-induced damage of one or more reactants in an illuminated analytical reaction at a given reaction time. In preferred embodiments, the reaction mixture is subjected to at least one illuminated and non-illuminated period and allowed to proceed such that the time in which the reaction mixture is illuminated is less than a photo-induced damage threshold period. | 08-11-2011 |
| 20110195407 | NUCLEIC ACID OCCURRING IN BLOOD FROM HEPATITIS PATIENT, POLYPEPTIDE ENCODED BY THE NUCLEIC ACID, AND USES OF THE SAME - A substance associated with cryptogenic hepatitis is found and information, particularly genetic information, on the substance is provided. Also, a method for detecting the substance and a material which can be used in the method are provided. A nucleic acid is extracted from each of 500 blood samples which have alanine aminotransferase (ALT) abnormality and are negative for a hepatitis virus marker, and the amplification of the nucleic acid is carried out by using helicase family-reactive helicase primers which have been produced uniquely. As a result, a novel nucleotide sequence (SEQ ID NO:1) can be obtained, and it is found that the nucleotide sequence occurs at high frequency in cryptogenic hepatitis. | 08-11-2011 |
| 20110200987 | COMPOSITIONS AND METHODS FOR MODIFYING CELLULAR PROPERTIES - The invention generally provides compositions and methods for modulating cell properties and enhancing recombinant protein yields. In particular, the compositions and methods provide for cells having altered growth characteristics, including altered adhesion, rate of proliferation, growth to particular cell density, and recombinant protein expression level. | 08-18-2011 |
| 20110200988 | 3'-OH unblocked uncleotides and nucleosides base modified with non-cleavable, terminating groups and methods for their use in DNA squencing - Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a non-cleavable terminating group. The non-cleavable-fluorescent group is designed to terminate DNA synthesis so that DNA oligomers can be sequenced efficiently in a parallel format. These reagents and methods will lead to more accurate identification of polymorphisms and other valuable genetic information. | 08-18-2011 |
| 20110200989 | SINGLE MOLECULE NUCLEIC ACID SEQUENCING USING MULTIPHOTON FLUORESCENCE EXCITATION - A system for detection of nucleic acids can include an excitation source configured to transmit excitation energy to a reaction site including a single molecule of nucleic acid reacted with a two-photon absorption moiety. The system also can include an optical system configured to focus the excitation energy transmitted from the excitation source to a focal region containing the reaction site, wherein said excitation energy within the focal region is sufficient to cause two-photon absorption by the two-photon absorption moiety. The system can further include a detector configured to detect emissions generated at the reaction site resulting from two-photon absorption of the excitation energy by the two-photon absorption moiety. | 08-18-2011 |
| 20110200990 | RECEPTOR ACTIVATOR OF NF kappa-B - Isolated receptors, DNAs encoding such receptors, and pharmaceutical compositions made therefrom, are disclosed. The isolated receptors can be used to regulate an immune response. The receptors are also useful in screening for inhibitors thereof. | 08-18-2011 |
| 20110200991 | AUTOMATED SYSTEM FOR ISOLATING, AMPLIFYING AND DETECTING A TARGET NUCLEIC ACID SEQUENCE - A system and method for preparing and testing of targeted nucleic acids is presented. The system integrates a pipetter, extractor, assay reader, and other components, including a selectively compliant articulated robot arm (SCARA). This synergistic integration of previously separate diagnostic tools creates a system and method whereby a minimum of human intervention is required. The resulting system provides a substantially more accurate and precise method of isolating, amplifying and detecting targeted nucleic acids for diagnosing diseases. | 08-18-2011 |
| 20110200992 | Biological Compositions, Articles and Methods for Monitoring Sterilization Processes - A sterility indicating composition comprising a plurality of sterilization process resistant spores; a germination medium comprising a sub-lethal amount of at least one cell-permeant nucleic acid-interacting fluorescent dye and at least one nutrient for germination of the spores; wherein the at least one cell-permeant fluorescent dye can interact with nucleic acids present in and produced by the plurality of spores during germination or during germination and outgrowth of the spores to produce an increase in fluorescence intensity, indicating that viable spores are present, and wherein the cell-permeant fluorescent dye is sufficiently stable at least at a temperature for incubating the spores to produce the increase in fluorescence intensity, a sterilization process indicator comprising the composition, and a method of determining the effectiveness of a sterilization process using the composition and indicator are disclosed. | 08-18-2011 |
| 20110207116 | SPATIO-TEMPORAL CONTROL OF PROTEIN INTERACTIONS USING PHYTOCHROMES - The invention provides methods, materials and systems of regulating association between proteins of interest using light. In an aspect, the invention takes advantage of the ability of phytochromes to change conformation upon exposure to appropriate light conditions, and to bind in a conformation-dependent manner to cognate proteins called phytochrome-interacting factors. The invention comprises a method of regulating interaction between a first protein of interest and second protein within a cell by light. Such a method optionally comprises providing in the cell (1) a first protein construct which comprises the first protein, a phytochrome domain (PHD), and (2) providing in the cell a second protein construct which comprises the second protein and a phytochrome domain-interacting peptide (PIP) that can bind selectively to the Pfr state, but not to the Pr state, of the phytochrome domain. | 08-25-2011 |
| 20110207117 | GENERATION OF PRODUCTION STRAINS THAT EFFICIENTLY EXPRESS NUCLEAR TRANSGENES - The present invention relates to a method of generating eukaryotic cells suitable for the expression of transgenes in said cells comprising (a) introducing a nucleic acid encoding a selectable marker responsive to a selecting agent into the nucleus of cells, wherein the level of expression of said selectable marker is proportional to the level of phenotypic responsiveness to said selecting agent; (b) selecting, among the cells obtained in step (a), for cells with a detectable expression of said selectable marker; (c) optionally propagating the cells selected for in step (b); (d) mutagenizing the cells selected for in step (b) or propagated in step (c) or allowing for the appearance of spontaneous mutations in the cells selected for in step (b) or propagated in step (c); and (e) selecting for cells displaying an increased expression of said selectable marker compared to the expression obtained in step (b). The present invention furthermore relates to a eukaryotic cell produced by the method of the present invention, a method of producing a compound of interest in a cell produced with the method of the present invention comprising (a) introducing a nucleic acid encoding (i) the compound of interest which is a protein or an RNA; or (ii) a protein necessary to synthesize said compound of interest; and optionally a selectable marker responsive to a selecting agent into said cell; (b) expressing said protein in the cell; and (c) isolating the compound of interest produced; and a kit comprising (a) a cell obtainable by the method of the invention and optionally a vector optimized for protein expression in said cell; or (b) the cell of the invention. | 08-25-2011 |
| 20110207118 | DNA TEMPLATE TAILORING USING PNA AND MODIFIED NUCLEOTIDES - Disclosed is a method whereby a repetitive nucleic acid sequence, such as a short tandem repeat (STR), may be characterized as to its length. Pyrosequencing is used to sequence an STR repetitive region to measure the length of STRs in a rapid manner. A combinatorial approach is disclosed for the addition of multiple nucleotides (e.g., two mononucleotides) at a time by the polymerase, which reduces the sample analysis time by half. In addition, modified nucleic acids, such as peptide nucleic acids, are used as blocking probe to stop polymerization on the flanking region which makes it possible to use pyrosequencing for DNA length measurement both in the case of homozygous or heterozygous samples for varying repeat patterns of different markers. Further, dideoxynucleotides are added to stop polymerization in the flanking region of the STR. | 08-25-2011 |
| 20110207119 | METHODS FOR PREDICTING A CANCER PATIENT'S RESPONSE TO SUNITINIB - The present invention provides methods for individualizing chemotherapy for cancer treatment, and particularly for evaluating a patient's responsiveness to sunitinib prior to treatment. The method comprises expanding malignant cells in culture from a patient's tumor specimen, contacting the cultured cells with one or more active agents including sunitinib, and evaluating or quantifying the response to the active agent(s). The result of the assay is a dose response curve, which may be evaluated using algorithms described herein, so as to quantitatively assess drug sensitivity. The in vitro response to the drug as determined by the method of the invention is correlative with the patient's in vivo response upon receiving sunitinib during chemotherapeutic treatment, in the course of standardized or individualized chemotherapeutic regimen. | 08-25-2011 |
| 20110207120 | WNT1 AS A RENAL DAMAGE BIOMARKER - The present invention comprises the use of WNT1 as a biomarker in the monitoring, prognosis and/or in the diagnosis of chronic nephropathies. It provides in vitro prognostic and diagnostic methods and kits for its implementation. The present invention also comprises the use of WNT1 in screening active ingredients for the manufacture of a drug for therapies for chronic nephropathies and a method for conducting said screening. | 08-25-2011 |
| 20110207121 | SAMPLE PROCESSING DEVICE FOR PRETREATMENT AND THERMAL CYCLING - A sample processing device may include an opening, a sample pretreatment unit, a thermal cycling reaction unit, and a detection unit. | 08-25-2011 |
| 20110212436 | MOLECULAR REDUNDANT SEQUENCING - Methods, systems and compositions where a target nucleic acid includes a registration sequence disposed therein for identification of the number or relative position of determined sequence from the template sequence. Particularly preferred aspects include a registration sequence in a circular template nucleic acid sequence which is, in turn, used in sequence by incorporation processes that rely upon template dependent, polymerase mediated primer extension in the identification of the sequence of the template. | 09-01-2011 |
| 20110212437 | SINGLE MOLECULE SEQUENCING WITH TWO DISTINCT CHEMISTRY STEPS - Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3′ hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3′ hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3′ hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids. | 09-01-2011 |
| 20110212438 | Kits and Devices for Performing Methods of Detecting Viability-Associated Molecules - Kits and devices for performing a method of detecting a molecule associated with viability of one or more cells or organisms in a sample are provided. The method comprises the initial step of contacting the sample with an enzyme, which enzyme is capable of adding or removing a chemical moiety to or from a nucleic acid molecule in the presence of the molecule associated with viability of the one or more cells or organisms. This thereby generates a novel detectable nucleic acid molecule. The next step involves detecting the presence of the molecule associated with viability of the one or more cells or organisms by detecting the novel nucleic acid molecule generated only in the presence of the molecule associated with viability of the one or more cells or organisms. A most preferred molecule associated with viability is ATP, although NAD may also be detected. A preferred enzyme for use in the methods is ligase. The kits and devices for performing the method have numerous applications, in particular in monitoring viability of cells, toxicology testing and determining whether there is contamination in a sample or on a surface. | 09-01-2011 |
| 20110212439 | HLA ALLELES ASSOCIATED WITH ADVERSE DRUG REACTIONS AND METHODS FOR DETECTING SUCH - This invention relates to a method of determining the presence of certain HLA alleles, such as HLA-B*1502 or HLA-B*5801, and a kit for carrying out this method. Also disclosed is a method for assessing whether a patient is at risk for developing adverse drug reactions (e.g., Stevens-Johnson syndrome, toxic epidermal necrolysis, or hypersensitivity syndrome) based on the presence or absence of a genetic marker (e.g., HLA-B*1502, HLA-B*5801, or HLA-B*4601). | 09-01-2011 |
| 20110212440 | CELL SORTING DEVICE - An integrated microsystem, comprising: a microchannel, a field generator to create a magnetic field in at least one first portion of the microchannel having a direction substantially collinear with the direction of flow in the portion of the microchannel, the magnetic field also presenting a gradient, wherein the microsystem additionally comprises a detection area in fluid connection with the microchannel, | 09-01-2011 |
| 20110217696 | AZA-BENZAZOLIUM CONTAINING CYANINE DYES - Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms. | 09-08-2011 |
| 20110217697 | METHODS AND APPARATUS FOR MEASURING ANALYTES USING LARGE SCALE FET ARRAYS - Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in the concentration of inorganic pyrophosphate (PPi), hydrogen ions, and nucleotide triphosphates. | 09-08-2011 |
| 20110217698 | METHOD TO IMPROVE SINGLE MOLECULE ANALYSES - The quality of information from single molecule analyses is improved by employing a method in which a single molecule reaction carried out within an optical confinement is monitored, the single molecule reaction is halted, and data from the optical confinement is obtained while the reaction is not occurring. Characteristic optical behavior observed while the reaction is halted is used to improve the quality of information obtained during the single molecule reaction, for example, by correcting the reaction data, excluding the reaction data, or providing a confidence level to the reaction data. | 09-08-2011 |
| 20110217699 | Fluorescent Chemical Compounds Having High Selectivity for Double Stranded DNA, and Methods for Their Use - Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA. | 09-08-2011 |
| 20110217700 | METHODS OF SEED BREEDING USING HIGH THROUGHPUT NONDESTRUCTIVE SEED SAMPLING - The present invention provides for novel methods to facilitate germplasm improvement activities through the use of high throughput, nondestructive sampling of seeds. A method for analyzing a population of haploid seeds generally includes providing a population of seeds comprising haploid seeds, removing tissue samples from a plurality of the seeds in the population using an automated seed sampler system while preserving the germination viability of the sampled seeds, and analyzing the tissue samples for the presence or absence of one or more characteristics indicative of at least one genetic or chemical trait. | 09-08-2011 |
| 20110223586 | OPTICAL PARTICLE CHARACTERIZATION SYSTEM - A particle characterization system including a flow source to produce a stream of particles; a source of light or other energy directed at the stream of particles to cause fluorescence or scattered light to be emitted from the particles; a detector of the fluorescent or scattered light including three or more light receivers in a non-planar arrangement to detect the light emitted by the particles; and a computer which receives information from the light detector regarding light emitted by a particle in response to the source of light or other energy, the computer programmed to determine a characteristic of the particle based on the light collected from the particle. | 09-15-2011 |
| 20110223587 | OPTICAL PARTICLE CHARACTERIZATION SYSTEM - A particle characterization system including a flow source to produce a stream of particles; a source of light or other energy directed at the stream of particles to cause fluorescence or scattered light to be emitted from the particles; a detector of the fluorescent or scattered light including a plurality of light receivers in an enclosed three-dimensional arrangement to detect the light emitted by the particles; and a computer which receives information from the detector regarding light emitted by a particle in response to the source of light or other energy, the computer programmed to determine a characteristic of the particle based on the light collected from the particle. | 09-15-2011 |
| 20110223588 | Solid Phase Nucleic Acid Extraction From Small Sample Volumes, and Release of Controlled Quantities - Products for and a method of capturing and storing nucleic acid from patient blood, plants or other samples (e.g., purified DNA or RNA), for use in analysis of nucleic acid are described. The products are made by heating a mixture of magnetic beads, silica particles, alkyl silicate (e.g., Silbond 4, Silbond Corp., Weston Mich.) and polyethylene resin particles. The quantity of nucleic acid adsorbed by the product is controlled by the surface area of silica available for binding to nucleic acid, which in turn is controlled by: the overall volume of the product, the ratio of the volume of polyethylene resin particles to the volumes of silica particles and alkyl silicate; and the sizes of silica particles (smaller particles have a larger surface area per unit volume). Controlling and limiting of the amount of nucleic acid captured by the product avoids the disadvantages associated with excess DNA/RNA for PCR amplification. | 09-15-2011 |
| 20110223589 | Solid Phase Nucleic Acid Extraction From Leukoreduced Blood - Products for and a method of capturing and storing nucleic acid from leukoreduced patient blood, where the products capture sufficient quantities for use in PCR and analysis of nucleic acid, are described. The products are made by heating a mixture of magnetic beads, silica particles, alkyl silicate (e.g., Silbond 4, Silbond Corp., Weston, Mich.) and polyethylene resin particles. The quantity of nucleic acid adsorbed by the product is controlled by the surface area of silica available for binding to nucleic acid, which in turn is controlled by: the overall volume of the product, the ratio of the volume of polyethylene resin particles to the volumes of silica particles and alkyl silicate; and the sizes of silica particles (smaller particles have a larger surface area per unit volume). | 09-15-2011 |
| 20110223590 | SINGLE-MOLECULE DETECTION SYSTEM AND METHODS - Embodiments encompass a single-molecule detection system and methods of using the detection system to detect an object. Further, embodiments encompass a detection system comprising a movable light coupler, a waveguide, and a light detector. Embodiments further encompass methods of single-molecule detection, including methods of single-molecule nucleic acid sequencing. | 09-15-2011 |
| 20110223591 | METHODS AND DEVICE TO CONSTRAIN MULTICELLULAR ARRANGEMENTS IN STABLE, STATIONARY AND REPRODUCIBLE SPATIAL CONFIGURATION - The present invention relates to methods and devices to obtain multicellular arrangements in stable, stationary and reproducible spatial configuration, and optionally with controlled internal cell organization, methods for preparing such devices, methods for studying the cells shapes, the cells architectures, the cells mechanical equilibrium, the cell-cell interaction, the cell movement and migration, the cell differentiation, the global internal cells organization, the cells polarities and division, and/or any function of cells, methods for screening compounds of interest which enhance or inhibit specific cell functions. | 09-15-2011 |
| 20110229877 | ENZYME-PORE CONSTRUCTS - The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing. | 09-22-2011 |
| 20110229878 | HUMAN CONSENSUS SODIUM-IODIDE SYMPORTER REPRESSOR (NIS-REPRESSOR) BINDING SITE - The present disclosure relates to a Sodium-Iodide Symporter-repressor (NIS-repressor) binding site (NRBS) consensus sequence consisting of a DNA molecule having the sequence: 5′-T/C(G/A)GCCT(T/C)A(G/A)TTTCCCCA(T/C)CTGT-3.′ The disclosure further relates to methods of screening compounds and other molecules that bind to or inhibits the NIS-repressor or inhibits or interferes with the binding of NIS-repressor to the NIS-repressor binding site. | 09-22-2011 |
| 20110229879 | METHODS AND COMPOSITIONS FOR NUCLEAR STAINING - The present invention relates to compositions, methods, and kits suitable for detecting nucleic acids in a biological sample. The nuclear staining composition of the present invention contains a pH buffering reagent, a solubilizing reagent, a basic dye, and an aqueous medium. The composition can be used alone to detect nucleic acids in a biological sample or in combination with other histological dyes for nuclear counterstaining. | 09-22-2011 |
| 20110229880 | GENE SILENCING - The invention provides a mirtron or a gene capable of expressing a mirtron for use in modifying the expression of a target gene in a mammalian cell to prevent or treat a disease, the sequence of the mirtron comprising: (i) a 5′ splice site; (ii) a 3′ splice site; (iii) a branch-point recognition sequence; (iv) a 3′ polypyrimidine tract greater than 15 nucleotides in length; and (v) an antisense sequence that is at least partially complementary to a sequence in the target gene. | 09-22-2011 |
| 20110229881 | DNA-CONTAINING INK COMPOSITION - An ink, which contains DNA and is hardly decomposed by an external stimulus such as ultraviolet light, heat, an acid or an alkali, is provided. A method of easily analyzing a DNA in an ink composition is also provided. An ink composition containing a DNA and having a water-tolerance at a certain level or higher is prepared. The DNA in a print that is produced by using the above ink composition is quickly extracted with water or an aqueous solution and analyzed. Furthermore, a DNA in a print that is produced by using an oil- and water-based ink composition containing the DNA is quickly extracted with water or an aqueous solution and analyzed. | 09-22-2011 |
| 20110229882 | DIAGNOSING AND MONITORING RESPONSE TO TREATMENT OF SOLID ORGAN TISSUE DISEASE - Techniques for diagnosing and monitoring response to treatment of a solid organ tissue disease in a patient are provided. For example, a technique for diagnosing a solid organ tissue disease in a patient includes obtaining at least one of one or more blood-derived nucleated acellular components and one or more nucleated cellular components from the patient to provide a reporter function in the patient. Also, a technique for monitoring response to treatment of a solid organ tissue disease in a patient includes obtaining at least one of one or more blood-derived nucleated acellular components and one or more nucleated cellular components from the patient to provide a reporter function in the patient. | 09-22-2011 |
| 20110236885 | Genetic Variants Predicting Warfarin Sensitivity - We discovered that a polymorphism in the promoter of the VKORC1 gene is associated with warfarin sensitivity. This polymorphism can explain both the inter-individual and inter-ethnic differences in warfarin dose requirements. Furthermore, the polymorphism is also associated with promoter activity. Thus, the promoter sequence or activity of the VKORC1 gene of a subject can be used to predict how much warfarin should be prescribed for the subject. Relevant methods and compositions are provided. | 09-29-2011 |
| 20110236886 | PATHOGENICITY DETERMINANTS WHICH CAN BE USED AS TARGETS FOR DEVELOPING MEANS FOR PREVENTING AND CONTROLLING BACTERIAL INFECTIONS AND/OR SYSTEMIC DISSEMINATION - The invention relates to a method for identifying and selecting a gene required for the proliferation in vivo of a pathogenic microorganism, comprising:—using a strain of the pathogenic microorganism,—generating mutants for inactivation in the genes encoding these factors,—determining the virulence of these mutants on an experimental model of infection, and their effect on enteric colonization in an axenic mouse model, and—selecting the bacterial genes essential for resistance to serum in vitro, and essential, in the host, for dissemination in the serum. Application to the screening of compounds which inhibit the products of the genes identified, and to the inhibition in vitro of the proliferation of a pathogenic microorganism in serum. | 09-29-2011 |
| 20110236887 | MODIFIED ACTINOMYCIN-BASED NUCLEIC ACID STAINS AND METHODS OF THEIR USE - Actinomycin-based near IR emitting compounds and methods of their use as nucleic acid stains are provided. | 09-29-2011 |
| 20110236888 | METHOD FOR ACCESSING THE CONTENTS OF A CLOSED VESSEL CONTAINING A SPECIMEN RETRIEVAL DEVICE - Method for obtaining a fluid from a collection device assembled to isolate a specimen retrieval device from the pathway of a fluid transfer device used to penetrate a cap of the collection device and to draw and remove the fluid from the collection device for analysis. | 09-29-2011 |
| 20110236889 | METHODS OF SCREENING FOR COMPOUNDS FOR USE AS MODULATORS OF LEFT-RIGHT ASYMMETRY IN SCOLIOTIC SUBJECTS AND FOR MONITORING EFFICACY OF AN ORTHOPAEDIC DEVICE - A method of screening for a compound for treating or preventing adolescent idiopathic scoliosis (AIS), said method comprising: (a) contacting a test compound with a paraspinal skin fibroblast or a paraspinal muscle cell sample from the right and/or left side of the spine of a subject; and (b) determining at least one of Nodal, Notch1, Pitx2, Lefty1 and Lefty2's expression and/or activity in the cell sample; wherein the test compound is selected as potentially useful in treating or preventing AIS if at least one of Nodal, Notch1, Pitx2, Lefty1 and Lefty2's expression and/or activity in the cell sample is different in the presence of the test compound as compared to in the absence thereof. | 09-29-2011 |
| 20110244446 | RNA SEQUENCE-SPECIFIC MEDIATORS OF RNA INTERFERENCE - The present invention relates to a Drosophila in vitro system which was used to demonstrate that dsRNA is processed to RNA segments 21-23 nucleotides (nt) in length. Furthermore, when these 21-23 nt fragments are purified and added back to Drosophila extracts, they mediate RNA interference in the absence of long dsRNA. Thus, these 21-23 nt fragments are the sequence-specific mediators of RNA degradation. A molecular signal, which may be their specific length, must be present in these 21-23 nt fragments to recruit cellular factors involved in RNAi. This present invention encompasses these 21-23 nt fragments and their use for specifically inactivating gene function. The use of these fragments (or chemically synthesized oligonucleotides of the same or similar nature) enables the targeting of specific mRNAs for degradation in mammalian cells, where the use of long dsRNAs to elicit RNAi is usually not practical, presumably because of the deleterious effects of the interferon response. This specific targeting of a particular gene function is useful in functional genomic and therapeutic applications. | 10-06-2011 |
| 20110244447 | COGNATE SAMPLING KINETICS - Provided are methods and compositions for measuring the transient binding of nucleotides and nucleotide analogs under conditions where the nucleotides or nucleotide analogs are unincorporable. The transient binding can be determined under single molecule observation conditions providing information about the kinetics of nucleotide analog sampling of the active site of the enzyme. The methods can be used for polymerase enzyme development, mechanistic understanding, and drug discovery. | 10-06-2011 |
| 20110244448 | DNA DETECTING APPARATUS, DNA DETECTING DEVICE AND DNA DETECTING METHOD - A chemiluminescence detecting apparatus includes a thing called a plate where many reaction chambers are one-dimensionally or two-dimensionally arranged, and enzymes packed in a gel and DNA-immobilized beads are simultaneously charged into the reaction chambers, thereby trapping a large amount of enzymes in the reaction chambers, improving enzyme activity and achieving high sensitive pyrosequencing. | 10-06-2011 |
| 20110244449 | METHODS OF DETERMINING COPY NUMBER OF A GENETIC LOCUS - Disclosed are methods of determining in a sample, the molar ratio of a query locus to a reference locus, comprising: 1) forming one or more mixtures, each mixture comprising: a) a reference-query coupled probe comprising a nucleobase polymer comprising i) a probe reference locus comprising a probe reference allele, and ii) a probe query locus comprising a probe query allele, and b) a sample comprising i) a sample reference locus comprising a sample reference allele and ii) 0 or greater copies of a sample query locus comprising a sample query allele; 2) for each mixture, determining a) the amount of probe reference allele as a fraction of total reference allele and b) the amount of probe query allele as a fraction of total query allele; and 3) calculating the molar ratio of the sample query allele to the sample reference allele. | 10-06-2011 |
| 20110244450 | PRIMATE TOTIPOTENT AND PLURIPOTENT STEM CELLS PRODUCED BY SOMATIC CELL NUCLEAR TRANSFER - Purified totipotent stem cells and pluripotent stems cells derived by somatic cell nuclear transfer are disclosed herein, as well as cell lines, multipotent cells and differentiated cells produced from these stem cells. The stem cells are produced from an enucleated host cell from a first donor and nuclear genetic material from a somatic cell of a second donor. Methods for making and using such compositions of such stem cells are also provided. | 10-06-2011 |
| 20110250589 | BIOMARKERS FOR LUNG DISEASE MONITORING - The present invention pertains to the monitoring and treatment of lung transplant recipients. In particular, the invention pertains to the use of biomarkers to predict or detect post-lung transplantation complications (e.g., organ rejection, acute organ rejection, organ injury, bronchiolitis obliterans, bronchiolitis obliterans syndrome, organizing pneumonia), fibroproliferative repair responses, interstitial lung diseases (e.g., idiopathic pulmonary fibrosis and other fibrotic lung diseases), and other immune-mediated lung diseases (e.g., graft versus host disease, scleroderma). | 10-13-2011 |
| 20110250590 | MULTIPLE MESODERMAL LINEAGE DIFFERENTIATION POTENTIALS FOR ADIPOSE TISSUE-DERIVED STROMAL CELLS AND USES THEREOF - The invention relates to methods and compositions for the differentiation of stromal cells from adipose tissue into hematopoietic supporting stromal cells and myocytes of both the skeletal and smooth muscle type. The cells produced by the methods are useful in providing a source of fully differentiated and functional cells for research, transplantation and development of tissue engineering products for the treatment of human diseases and traumatic tissue injury repair. | 10-13-2011 |
| 20110250591 | METHOD OF DESIGNING siRNAS FOR GENE SILENCING - The present invention provides a method for identifying siRNA target motifs in a transcript using a position-specific score matrix approach. The invention also provides a method for identifying off-target genes of an siRNA using a position-specific score matrix approach. The invention further provides a method for designing siRNAs with higher silencing efficacy and specificity. The invention also provides a library of siRNAs comprising siRNAs with high silencing efficacy and specificity. | 10-13-2011 |
| 20110250592 | METHODS FOR DETERMINING A PATIENT'S SUSCEPTIBILITY OF CONTRACTING A NOSOCOMIAL INFECTION AND FOR ESTABLISHING A PROGNOSIS OF THE PROGRESSION OF SEPTIC SYNDROME - A method for determining a patient's susceptibility of contracting a nosocomial infection that includes obtaining a biological sample from the patient and extracting biological material from the biological sample; preparing a specific reagent of an expression product of at least one target gene selected from S100A9 and S100A8 target genes; and determining the expression of at least one of the target genes S100A9 and S100A8, where overexpression relative to a specified threshold value indicates susceptibility of contracting a nosocomial infection. | 10-13-2011 |
| 20110250593 | TESTING LUMENECTOMY SAMPLES FOR MARKERS OF NON-VASCULAR DISEASES - Lumenectomy material is tested to determine the presence or likelihood of a condition of a patient. The lumenectomy material is in the form of at least one continuous tissue strand collected in vivo from an inner surface of a body lumen of the patient. The presence of at least one marker of a disease is determined. The disease may be hypertension, hyperlipidemia, depression, obesity, metabolic syndrome, insulin resistance, kidney damage, or diabetes. The patient is identified as having or as likely to develop the disease if a marker of the disease is identified in the lumenectomy material of the patient. | 10-13-2011 |
| 20110256529 | BEADS FOR USE IN REACTIONS FOR THE AMPLIFICATION AND/OR SYNTHESIS OF A POLYNUCLEOTIDE, AND A DEVICE AND A METHOD FOR THE PRODUCTION THEREOF - A method is provided of making meltable beads for use in reactions for the amplification and/or synthesis of a polynucleotide, the method comprising (i) Providing one or more reagents for use in a reaction for the amplification and/or synthesis of a polynucleotide and a substance for promoting the formation of a solid bead; (ii) Providing a reactor device comprising a sample conduit and a first carrier fluid conduit for the flow of immiscible liquids, the sample conduit and first carrier fluid conduit meeting at a junction; (iii) Heating the one or more reagents for use in a reaction for the amplification and/or synthesis of a polynucleotide and the substance for promoting the formation of a solid bead so as to form a liquid comprising the one or more reagents for use in a reaction for the amplification and/or synthesis of a polynucleotide and the substance for promoting the formation of a solid bead in intimate admixture; (iv) Passing the liquid down the sample conduit and a carrier fluid down the first carrier fluid conduit, thus causing the formation of droplets at or downstream of the junction and (v) Causing the formation of solid beads by cooling the droplets. A device for use in such methods | 10-20-2011 |
| 20110256530 | PRODUCTS AND METHODS FOR TISSUE PRESERVATION - The present application relates to methods for increasing stability of formalin fixed, optionally paraffin embedded biological material. In one example, DNAse inhibitors and/or RNAse inhibitors are combined with the biological material or formalin at the time of fixation or shortly prior. | 10-20-2011 |
| 20110256531 | BIODETECTION ARTICLES | 10-20-2011 |
| 20110256532 | ANALYZER - An object of the present invention relates to providing a nucleic acid analyzer capable of testing a plurality of test items in parallel, and of obtaining high efficiency of specimen processing even if the test item or a measuring object is changed. The present invention relates to an analyzer including a carousel rotatable about a rotation axis, a plurality of reaction containers held along a circumferential edge of the carousel, and at least one detector having a light source for irradiating the reaction container with excitation light and a detection element for detecting fluorescence from a reaction liquid in the reaction container. The detector is removable. By attaching a desired detector, it is possible to perform fluorescence measurement in response to the test item. According to the present invention, it is possible to test a plurality of test items in parallel, and even if the test item or the measuring object is changed, the high efficiency of specimen processing can be obtained. | 10-20-2011 |
| 20110262898 | Compositions, Methods, and Kits for Amplifying Nucleic Acids - The present teachings are directed to compositions, methods, and kits for amplifying target nucleic acids while reducing non-specific fluorescence and undesired amplification products, sometimes referred to as secondary amplification products or spurious side-products. The enzyme inhibitors disclosed herein comprise a nucleotide sequence and at least one quencher. Complexes comprising an enzyme inhibitor associated with an enzyme, wherein at least one enzymatic activity of the enzyme is inhibited, are also provided. Methods for amplifying a target nucleic acid while reducing undesired amplification products are disclosed, as are methods for reducing non-specific fluorescence. Kits for expediting the performance of certain disclosed methods are also provided. | 10-27-2011 |
| 20110262899 | FLUORESCENT LABELING OF TRANSFER RNA AND STUDY OF PROTEIN SYNTHESIS - Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system. The disclosed methods are useful in single molecule as well as in ensemble settings. | 10-27-2011 |
| 20110262900 | Male Reproductive Health Panel and Uses Thereof - A male reproductive health screening panel is disclosed. In some embodiments, the method comprises screening human sperm using a sperm DNA accelerated decondensation (SDAD) Test, and/or a Sperm DNA decondensation (SDD) assay, in combination with an assessment of the sperm DNA fragmentation index (DFI) of the sperm sample of interest. The method is useful in the screening and clinical management for a reproductively challenged human couple, in the assessing the reproductive health of a potential sperm donor, and in selecting an appropriate assisted reproductive technique (ART) for a reproductively challenged couple. These methods provide a decision tree that employs a patient's sperm test results in a SDAD Test, sperm DFI and/or SDD Test. An automated scoring method is disclosed for assessing sperm activity after SDAD and SDD tests. Also disclosed is an automated sperm processing protocol for both the SDD and SDAD tests. | 10-27-2011 |
| 20110262901 | SUSCEPTIBILITY VARIANTS FOR PERIPHERAL ARTERIAL DISEASE AND ABDOMINAL AORTIC ANEURYSM - The present invention discloses certain genetic variants as susceptibility variants for peripheral arterial disease (PAD) and abdominal aortic aneurysm (AAA). The invention relates to risk management using such variants. The invention further relates to kits for use in risk assessment of PAD and AAA. | 10-27-2011 |
| 20110262902 | CIS-ACTING DIVERSIFICATION ACTIVATOR AND METHOD FOR SELECTIVE DIVERSIFICATION OF NUCLEIC ACIDS - The invention relates to identification of a cis-acting diversification activator (DIVAC) that is necessary and sufficient for the activation of diversification in transcription units linked thereto. The invention provides a method for diversification of a target nucleic acid comprising introducing a genetic construct comprising the diversification activator into a recipient cell, wherein the diversification activator is linked to the target nucleic acid. | 10-27-2011 |
| 20110262903 | Modified Proteins and Methods of Making and Using Same - Methods, compositions, systems, apparatuses and kits comprising modified proteins, particularly modified nucleic acid-binding proteins with altered buffering properties are provided. For example, in some embodiments, methods of forming modified proteins including one or more amino acid modifications to achieve desired pKa values are described. Furthermore, the invention provides methods for using such modified proteins in ion-producing reactions, such as ion-based nucleic acid sequencing reactions. | 10-27-2011 |
| 20110262904 | FLUORESCENT DYES,METHODS OF SYNTHESIS AND APPLICATIONS THEREOF - The present invention provides a category of cyanine dyes having the following general structural Formula I, wherein X is defined as C(CH | 10-27-2011 |
| 20110262905 | KIT FOR THE OPTIMISATION OF PROTEIN SYNTHESIS/SECRETION - The invention relates to a kit to be used for the identification of a secretion cassette, which in eukaryotic cells gives rise to a higher level of synthesis/secretion of a protein of interest, the coding sequence of which being flanked by the first and the second set of genetic elements, respectively, compared to the other secretion cassettes present in the kit containing the coding sequence at the corresponding position, as well as to a method wherein the kit is used and the use of said kit, whereby the best secretion cassette will be obtained. | 10-27-2011 |
| 20110262906 | MICROFLUIDIC MULTIPLEXED CELLULAR AND MOLECULAR ANALYSIS DEVICE AND METHOD - A sequential flow analysis tool comprising a microti iridic device having a fluid path defined within a substrate between an input and an output is described. The device includes a capture chamber provided within but offset from the fluid path, the capture chamber extending into the substrate in a direction substantially perpendicular to the fluid path such that operably particles provided within a fluid flowing within the fluid path will preferentially collect within the capture chamber. | 10-27-2011 |
| 20110262907 | PROCECURE FOR PREPARING A PROCESSED VIRTUAL ANALYSIS IMAGE - This method includes the following steps: carrying out a processing of the specimen so as to make it possible to differentiate the pathological cells from the healthy cells of the specimen; and performing an acquisition of images of the specimen disposed on an analysis plate so as to obtain a plurality of images each representing a zone of the analysis plate, the images placed side by side forming an image of the whole of the specimen so as to create a virtual analysis plate. The method furthermore includes the following step: performing on the virtual analysis plate a processing of the images acquired so as to obtain a virtual restitution of the colors and of the intensity of the colors of the cytoplasm and/or of the nucleus, the colors and the intensity being able to be modified according to the preferences of the person in charge of the analysis. | 10-27-2011 |
| 20110269119 | ENCODING TEXT INTO NUCLEIC ACID SEQUENCES - Methods and apparatus are disclosed herein for encoding human readable text conveying a non-genetic message into nucleic acid sequences with a substantially reduced probability of biological impact and decoding such text from nucleic acid sequences. In one embodiment, each symbol of a symbol set of human readable symbols uniquely maps to a respective codon identifier. Mapping may ensure that each symbol will not map to a codon identifier that generates an amino acid residue which has a single-letter abbreviation that is the equivalent to the respective symbol. Synthetic nucleic acid sequences comprising such human readable text, and recombinant or synthetic cells comprising such sequences are provided, as well as methods of identifying cells, organisms, or samples containing such sequences. | 11-03-2011 |
| 20110269120 | METHODS AND COMPOSITIONS FOR DETECTING PROMOTER ACTIVITY AND EXPRESSING FUSIONPROTEINS - The present invention provides nucleic acid molecules comprising one or more nucleic acid sequences encoding a polypeptide having a detectable activity. The present invention also provides methods of joining such nucleic acid molecules to nucleic acid molecules to be assayed for promoter activity. The present invention also relates to methods of preparing fusion proteins comprising a polypeptide of interest and a polypeptide having a detectable activity. | 11-03-2011 |
| 20110269121 | LAB-ON-A-PIPETTE - This invention generally relates to an integrated ‘Lab-on-a-Pipette™’ which will provide sample-to-answer single cell genetic diagnosis for preimplantation genetic diagnosis (PGD) and other forms of single cell analysis (SCA). SCA is a quickly growing field with substantial impact in prenatal testing, cancer biopsies, diabetes, stem cell research, and our overall understanding of heterogeneity in biology. However, single cell genetic analysis is challenging, inaccurate, and in many cases impossible, due to the small amount of sample (5 pg), and difficulties in handling small sample volumes (50-100 pL). The ‘lab-on-a-pipette’ device integrates a microaspiration tip with microfluidic analysis components to conduct in-situ, real-time single cell genetic diagnosis in a single device. The microaspiration tip extracts and encapsulate a cell into an ultra-low volume plug (˜300 pL). | 11-03-2011 |
| 20110269122 | HEREDITARY HEMOCHROMATOSIS GENE - The invention relates generally to the gene, and mutations thereto, that are responsible for the disease hereditary hemochromatosis (HH). More particularly, the invention relates to the identification, isolation, and cloning of the DNA sequence corresponding to the normal and mutant HH genes, as well as the characterization of their transcripts and gene products. The invention also related to methods and the like for screening for HH homozygotes and further relates to HH diagnosis, prenatal screening and diagnosis, and therapies of HH disease, including gene therapeutics, protein and antibody based therapeutics, and small molecule therapeutics. | 11-03-2011 |
| 20110275061 | ASSAYS USING SURFACE-ENHANCED RAMAN SPECTROSCOPY (SERS)-ACTIVE PARTICLES - Disclosed herein are diagnostic assays using surface enhanced Raman spectroscopy (SERS)-active particles, including liquid-based assays; magnetic capture assays; microparticle-nanoparticle satellite structures for signal amplification in an assay; composite SERS-active particles useful for enhanced detection of targets; and sample tubes and processes for using the same. | 11-10-2011 |
| 20110275062 | Systems And Methods For Integrating A Single DNA Molecule Into A Molecular Electronic Device - The disclosed subject matter provides a techniques for precisely and/or functionally cutting carbon nanotubes, e.g., single walled carbon nanotubes (“SWNTs”) and integrating a single nucleic acid molecule (e.g., a DNA molecule) into a gap formed into the carbon nanotubes. In one aspect, a method of fabricating a molecular electronic device includes disposing a SWNT on a base layer, forming a gap in the SWNT using a lithographic process, and disposing a single DNA strand across the gap so that each end of the nucleic acid contacts a gap termini. The disclosed subject matter also provides techniques for measuring the electrical properties (charge transport) of a DNA molecule which is integrated into an SWNT. Furthermore, a molecular electronic device including an SWNT with an integrated nucleic acid molecule is disclosed. | 11-10-2011 |
| 20110275063 | SYSTEMS AND METHODS OF DROPLET-BASED SELECTION - The present invention generally relates to fluidic droplets, and techniques for screening or sorting such fluidic droplets. In some embodiments, the fluidic droplets may contain cells (e.g., hybridoma cells) that can secrete various species, such as antibodies, for example. In one aspect, a plurality of fluidic droplets containing cells is screened to determine proteins, antibodies, polypeptides, peptides, nucleic acids, or the like. For example, cells able to secrete species such as antibodies may be se according to certain embodiments of the invention. Examples of such cells include, for instance, immortal cells such as hybridomas, or non-immortal cells such as B-cells. For instance, blood cells may be encapsulated within a plurality of fluidic droplets, and the cells able to produce antibodies may be determined. In some cases, expression or secretion levels may be determined using signaling entities, for example, determinable microparticles present within the fluidic droplet. Other aspects of the invention relate to kits involving such fluidic droplets, methods of promoting the making or use of such fluidic droplets, and the like. | 11-10-2011 |
| 20110275064 | METHOD FOR HEMATOLOGY ANALYSIS - A method whereby one or more fluorescent dyes are used to bind and stain nucleic acids in certain blood cells, such as, for example, white blood cells, nucleated red blood cells, and reticulocytes, and to induce fluorescent emissions upon excitation of photons from a given source of light, such as, for example, a laser, at an appropriate wavelength. More particularly, this invention provides a method whereby a fluorescent trigger is used in a data collection step for collecting events that emit strong fluorescence, in order to separate white blood cells and nucleated red blood cells from red blood cells and platelets without the need for using a lysing agent. | 11-10-2011 |
| 20110275065 | METHODS AND COMPOSITIONS FOR THE DIAGNOSIS AND TREATMENT OF THYROID CANCER - Methods for detecting thyroid cancer or thyroid cancer status in a subject are described comprising measuring novel markers or polynucleotides encoding the markers in a sample from the subject. The invention also provides localization or imaging methods for thyroid cancer, and kits for carrying out the methods of the invention. The invention also contemplates therapeutic applications for thyroid cancer employing the novel markers, polynucleotides encoding the markers, and/or binding agents for the markers. | 11-10-2011 |
| 20110275066 | METHOD OF DNA ANALYSIS USING MICRO/NANOCHANNEL - Methods are provided for tagging, characterizing and sorting double-stranded biomolecules while maintaining the integrity of the biomolecules. | 11-10-2011 |
| 20110275067 | ANALYSIS OF CHEMICALLY CROSSLINKED CELLULAR SAMPLES - A method of analyzing cellular samples that include a chemically crosslinked analyte is provided. The analysis typically involves the use of mass spectrometry. | 11-10-2011 |
| 20110281259 | Method And Apparatus For the Simultaneous, Automated Decomposition Of A Plurality Of Biological Samples - The invention relates to a method for decomposing a biological sample ( | 11-17-2011 |
| 20110281260 | RIBONUCLEIC ACID BINDING MOTIF PROTEIN 20 SEQUENCE VARIANTS - This document relates to methods and materials for using nucleic acid and amino acid sequence variants of ribonucleic acid binding motif protein 20 (RBM20). For example, methods and materials for using nucleic acid sequence variants and/or their corresponding amino acid variants of RBM20 that are associated with dilated cardiomyopathy to identify mammals (e.g., humans) at risk of having dilated cardiomyopathy that is likely to progress to heart failure are provided. | 11-17-2011 |
| 20110281261 | VECTORS - The present invention relates to a transfer vector for inserting a gene into a genetic locus of a baculovirus sequence. The transfer vector comprises an expression cassette comprising a eukaryotic promoter operably linked to the gene and a bipartite selection cassette. The present invention also relates to methods of using the transfer vector and derived bacmids and baculoviruses. | 11-17-2011 |
| 20110287411 | METHODS OF CHROMOSOME DRYING AND SPREADING - The present invention provides for a method of drying and spreading chromosomes from various biological samples to yield optimal chromosomal spreading. The method requires preparing a biological sample for treatment, providing a cytogenetic chamber capable of setting predetermined conditions, pre-testing a portion of the biological sample in the cytogenetic chamber, and finally treating the remaining biological sample. The method is useful to yield metaphase chromosomes that are small and rounded, with very few overlapping or scattered chromosomes. Furthermore, the method is uses restricted ranges of temperature and relative humidity to achieve consistent chromosomal spreading. The morphologies of the chromosomes are preserved in order to execute banding techniques at 550 bands and chromosomal analysis on high-resolution chromosomes. | 11-24-2011 |
| 20110287412 | Methods for Identifying RNA Segments Bound by RNA-Binding Proteins or Ribonucleoprotein Complexes - The present invention relates to a method for identifying a binding site on an RNA transcript, wherein the binding site binds to one or more binding moieties. The method includes, among other things, introducing a photoreactive nucleoside into living cells wherein the living cells incorporate the photoreactive nucleoside into RNA transcripts during transcription thereby producing modified RNA transcripts; reverse transcribing the RNA of isolated cross-linked segments thereby generating cDNA transcripts with one mutation wherein the photoreactive nucleoside is transcribed to a mismatched deoxynucleoside; amplifying the cDNA transcripts thereby generating amplicons; and analyzing the sequences of the amplicons aligned against the reference sequence so as to identify the binding site, wherein the sequences of each amplicon having a mutation resulting from the introduction of the photoreactive nucleoside is considered to be a valid amplicon comprising at least a portion of a binding site on the RNA transcript. | 11-24-2011 |
| 20110287413 | MICROFLUIDIC SYSTEM - A microfluidic system comprising a 1 | 11-24-2011 |
| 20110294114 | Optimization of determinants for successful genetic correction of diseases, mediated by hematopoietic stem cells - Methods and compositions disclosed herein generally relates to methods of determining minimum hematopoietic stem cell (HSC) chimerism and gene dosage for correction of a hematopoietic disease; in particular, in in vivo models. The invention also relates to modified lentiviral expression vectors for increase a viral titer and various methods for increasing such titers as well as expression vectors capable of enhancing such titers. The invention also relates to CHS4 chromatin insulator-derived functional insulator sequences. The invention further relates to methods for genetic correction of diseases or reducing symptoms thereof, such as sickle cell anemia, a lysosomal storage disease. The invention further relates to a method of improving and/or correcting one or more central nervous system (CNS) abnormalities caused by one or more lysosomal storage disease. The invention further relates to methods of improving titer in transfection-based bioreactor culture production or transfection-based production systems using eukaryotic cells. | 12-01-2011 |
| 20110294115 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 12-01-2011 |
| 20110294116 | METHODS AND SYSTEMS FOR DIRECT SEQUENCING OF SINGLE DNA MOLECULES - The invention provides improved methods for sequencing nucleic acids, e.g., for medical applications and biomedical research. The disclosed methods can be applied to rapid personalized medicine, genetic diagnosis, pathogen identification, and sequencing species genomes. | 12-01-2011 |
| 20110300532 | CONTROL OF THE TOXICITY OF GOLD NANOPARTICLES - The present invention relates to gold nanocluster compounds, especially gold nanoparticles, having a reduced toxicity, especially cytotoxicity, which are preferably appropriate for use in medical diagnostics such as medical imaging (e.g. X-ray, CT etc.), said gold nanocluster compounds comprising a core of at least one gold atom and at least one ligand bound to said core, wherein the reduction of toxicity, especially cytotoxicity, of said gold nanocluster compound is controlled and/or adjusted via its ligand structure and/or its ligand chemistry. | 12-08-2011 |
| 20110300533 | Yeast Screens for Treatment of Human Disease - Screening methods for identifying substances that provide therapeutic value for various diseases associated with protein misfolding are provided. Genetic and chemical screening methods are provided using a yeast system. The methods of the invention provide a rapid and cost-effective method to screen for compounds that prevent protein misfolding and/or protein fibril formation and/or protein aggregation which includes numerous neurodegenerative diseases including Parkinson's disease, Alzheimer's disease, Huntington's disease as well as non-neuronal diseases such as type 2 diabetes. | 12-08-2011 |
| 20110300534 | COMPOSITIONS AND METHODS FOR SEQUENCING NUCLEIC ACIDS - Embodiments relate to methods of sequencing nucleic acids. Embodiments encompass the use of nucleotide analogs and a nucleic acid polymerase enzyme or enzyme complex comprising proofreading activity. The nucleotide analogs may become incorporated into a replicating strand and induce the proofreading activity of the polymerizing enzyme, thereby prolonging the duration of a signal associated with nucleotide incorporation, resulting in more observable sequencing events and increasing the accuracy of nucleic acid sequencing. | 12-08-2011 |
| 20110306039 | Apparatus for single-molecule detection - An apparatus for detecting an object capable of emitting light. The apparatus comprises a light source and a waveguide. The waveguide comprises a core layer and a first cladding layer. At least one nanowell is formed in at least the first cladding layer. The apparatus further comprises a light detector. The light detector can detect a light emitted from a single molecule object contained in the at least one nanowell. | 12-15-2011 |
| 20110306040 | CPG RECEPTOR (CPG-R) AND METHODS RELATING THERETO - The present invention is directed to nucleic acid molecules and polypeptides encoding a CpG receptor (CpG-R). The CpG-R contains a THD, interacts with the MyD88 adapter protein, and may bind to CpG oligonucleotides. The present invention is also directed to antibodies against CpG-R and to methods of modulating an immune response and to methods of identifying compounds which bind to and/or modulate CpG-R. | 12-15-2011 |
| 20110306041 | DEVICE FOR CELL CULTURE - A device for cell culture, in particular of neuronal cells, including: a substrate defining a first microfluidic chamber to be seeded with a first cell culture, and at least a second microfluidic chamber, a fluidic interconnection system connecting the first and second chambers and enabling cellular extensions, in particular axons, to extend from one chamber to the other, wherein the interconnection system of the device is made so as to promote the progression of at least one first type of cellular extension, the first and second types of extension being different either due to the microfluidic chamber from which they originate, or due to the type of cell of which they constitute an extension. | 12-15-2011 |
| 20110306042 | DETERMINATION OF CHROMATIN CONFORMATION - Methods and kits for determining histone modification or epigenetic status are provided. | 12-15-2011 |
| 20110311963 | Method and Apparatus for Addressable Flow Cells in Single Molecule Sequencing - A method of sequencing a plurality of template nucleotide sequences includes immobilizing the plurality of template nucleotide sequences on a substrate. A first subset of the plurality of template nucleotide sequences is immobilized in a first field of view and a second subset of the plurality of template nucleotide sequences is immobilized in a second field of view. The first and second subsets are hybridized to a caged primer. The caged primer includes a caging group. The method further includes lysing the caging group from the caged primer in the first field of view and observing the first field of view to detect sequencing of the first subset of the plurality of template nucleotide sequences. | 12-22-2011 |
| 20110311964 | LABELED REACTANTS AND THEIR USES - Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions, that include structural components that maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated. | 12-22-2011 |
| 20110311965 | METHODS OF ENHANCING TRANSLOCATION OF CHARGED ANALYTES THROUGH TRANSMEMBRANE PROTEIN PORES - The invention relates to enhancing translocation of a charged analyte through a transmembrane protein pore. Translocation is enhanced by increasing the net opposing charge of the barrel or channel and/or entrance of the pore. The invention also relates to pores enhanced in accordance with the invention. | 12-22-2011 |
| 20110311966 | DETECTION CONJUGATE AND METHOD FOR ANALYSIS - The invention provides a combination of reagents as a test kit containing a detection conjugate having a binding portion and a reagent for deactivating of the fluorochrome portion by interaction with the linker. The binding portion especially is an antibody portion. The detection conjugate has a fluorochrome portion connected to the antibody portion, and a linker connected to the fluorochrome portion, wherein the linker comprises an oligonucleotide. The fluorochrome portion can be deactivated by hydrolysis of the linker or by specific hybridization of a quencher having an oligonucleotide to the linker. | 12-22-2011 |
| 20110318730 | MICROPATTERNED CO-CULTURE SYSTEMS AS INFECTIOUS DISEASE ANALYSIS PLATFORMS - Cell cultures are provided that include a population of micropatterened hepatocytes and one or more non-parenchymal cell populations, where the hepatocytes are infected with a virus or parasite and include a reporter of virus or parasite infection. Methods of making and using the cell cultures are also provided. | 12-29-2011 |
| 20110318731 | QUANTITATIVE HELICASE ASSAY - Disclosed herein are methods and kits relating to detection and quantitation of helicase activity. | 12-29-2011 |
| 20120003630 | MODULAR NUCLEIC ACID-BASED CIRCUITS FOR COUNTERS, BINARY OPERATIONS, MEMORY, AND LOGIC - We have created novel engineered genetic counter designs and methods of use thereof that utilize DNA recombinases to provide modular systems, termed single invertase memory modules (SIMMs), for encoding memory in cells and cellular systems. Our designs are easily extended to compute to high numbers, by utilizing the >100 known recombinases to create subsequent modules. Flexibility in our engineered genetic counter designs is provided by daisy-chaining individual modular components, i.e., SIMMs together. These modular components of the engineered genetic counters can be combined in other network topologies to create circuits that perform, amongst other things, logic and memory. Our novel engineered genetic counter designs allow for the maintenance of memory and provide the ability to count between discrete states by expressing the recombinases between their cognate recognition sites. | 01-05-2012 |
| 20120003631 | INSTRUMENT FOR CASSETTE FOR SAMPLE PREPARATION - A parallel processing system for processing samples is described. In one embodiment, the parallel processing system includes an instrument interface parallel controller to control a tray motor driving system, a close-loop heater control and detection system, a magnetic particle transfer system, a reagent release system, a reagent pre-mix pumping system and a wash buffer pumping system. | 01-05-2012 |
| 20120009567 | SEQUENCING REACTIONS WITH ALKALI METAL CATIONS FOR PULSE WIDTH CONTROL - Compositions, kits, methods and systems for single molecule nucleotide sequencing comprising producing polymerase reactions having monovalent cations that control the median pulse width for incorporated nucleotides are disclosed. The levels of alkali metals such as lithium, sodium, potassium, rubidium, and cesium in the polymerization are used to control pulse width while allowing other sequencing parameters to remain within a desirable range. | 01-12-2012 |
| 20120009568 | THERMOELECTRIC METHOD OF SEQUENCING NUCLEIC ACIDS - The present invention relates to a novel thermoelectric method for determining the sequence of nucleotides on a nucleic acid molecule through use of a thermopile and/or sequencing reagents flowing under the conditions of laminar flow. The methods disclosed herein involve the measurement of the heat generated by a deoxynucleotide incorporation event that can be accomplished without the need to control the temperature of any of a thermopile's junctions. | 01-12-2012 |
| 20120009569 | MICROORGANISM CONCENTRATION PROCESS AND DEVICE - A process for capturing or concentrating microorganisms for detection or assay comprises (a) providing a concentration device comprising a sintered porous polymer matrix comprising at least one concentration agent that comprises diatomaceous earth bearing, on at least a portion of its surface, a surface treatment comprising a surface modifier comprising ferric oxide, titanium dioxide, fine-nanoscale gold or platinum, or a combination thereof; (b) providing a sample comprising at least one microorganism strain; and (c) contacting the concentration device with the sample such that at least a portion of the at least one microorganism strain is bound to or captured by the concentration device. | 01-12-2012 |
| 20120015351 | miRNA Target Prediction - The present invention relates to generation (e.g., synthesis) of proteins. In particular, the present invention provides methods to predict miRNA targets using sequence similarity and thermodynamic stability of miRNA-bridges across both 3′ and 5′ UTR. Such methods find use in research, diagnostic and therapeutic settings (e.g., to discover targets, drugs, diagnostic products, etc.). | 01-19-2012 |
| 20120015352 | METHOD OF DETERMINING SENSITIVITY OF HUMAN OR NON-HUMAN ANIMAL CELLS TO AN IAP ANTAGONIST - cFLIP serves as a biomarker for efficacy of treatment with IAP antagonists, including Smac peptidomimetics. | 01-19-2012 |
| 20120015353 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 01-19-2012 |
| 20120015354 | NUCLEIC ACID APTAMER BINDER SPECIFICALLY TO BISPHENOL A - A nucleic acid aptamer capable of binding specifically to bisphenol A and a method of detecting and removing bisphenol A using the nucleic acid aptamer. The nucleic acid aptamer capable of binding specifically to bisphenol A has affinity for bisphenol A at nM concentration, and thus can detect even a very small amount of bisphenol A. Also, the nucleic acid aptamer can specifically detect only bisphenol A without showing affinity for other bisphenols, including bisphenol B having no difference from bisphenol A except for a single methyl group. Accordingly, the nucleic acid aptamer is effective in detecting and removing the environmental hormone bisphenol A which is difficult to detect by conventional methods. | 01-19-2012 |
| 20120021408 | FOUR-COLOR DNA SEQUENCING BY SYNTHESIS USING CLEAVABLE FLUORESCENT NUCLEOTIDE REVERSIBLE TERMINATORS - This invention provides a process for sequencing single-stranded DNA by employing a nanopore and modified nucleotides. | 01-26-2012 |
| 20120021409 | Common Light Chain Mouse - A genetically modified mouse is provided, wherein the mouse expresses an immunoglobulin light chain repertoire characterized by a limited number of light chain variable domains. Mice are provided that express just one or a few immunoglobulin light chain variable domains from a limited repertoire in their germline. Methods for making light chain variable regions in mice, including human light chain variable regions, are provided. Methods for making human variable regions suitable for use in multispecific binding proteins, e.g., bispecific antibodies, are provided. | 01-26-2012 |
| 20120021410 | TRIGGERED MOLECULAR GEOMETRY BASED BIOIMAGING PROBES - The present embodiments relate to engineering imaging probes based on “triggered molecular geometry.” Upon detection of a molecular signal, nucleic acid hairpin monomers assemble an imageable molecular shape with prescribed geometry. In some embodiments the prescribed shape can be imaged directly. In some embodiments, the prescribed shape can serve as a spatial organizer or amplification scheme for other imaging entities, such as fluorophore and fluorescent proteins. | 01-26-2012 |
| 20120021411 | AUTOMATED HIGH-THROUGHPUT SEED SAMPLER AND METHODS OF SAMPLING, TESTING AND BULKING SEEDS - A seed sampling system includes a seed transport configured to hold multiple individual seeds together as a group and transport the multiple individual seeds together as the group, and to allow the multiple individual seeds to be oriented, substantially simultaneously, while the multiple individual seeds are being held in the seed transport. The seed sampling system also includes a seed sampling subsystem configured to remove tissue from the oriented multiple individual seeds. And, a method for removing tissue from the multiple individual seeds includes loading the multiple individual seeds in the seed transport, orienting the multiple individual seeds in the seed transport substantially simultaneously, and removing tissue from the oriented multiple individual seeds. | 01-26-2012 |
| 20120028248 | ENGINEERED FLUORESCENT DYE LABELED NUCLEOTIDE ANALOGS FOR DNA SEQUENCING - Engineered nucleotide compositions, having polymerase interacting components that improve the interactivity of the polymerase and the nucleotide, particularly for nucleic acid sequencing applications. Compositions include the interactive polymerases along with the nucleotide analogs. Kits, methods and systems are provided for analysis of nucleoc acid synthesis reactions. | 02-02-2012 |
| 20120028249 | ANTHRAQUINONE BASED NEAR IR EMITTING COMPOUNDS AND USES THEREOF - Disclosed are near IR emitting fluorescent compounds; methods of making and kits containing the described compounds; and their use in fluorescence-based detection of biological materials. | 02-02-2012 |
| 20120028250 | MITIGATION OF PHOTODAMAGE IN ANALYTICAL REACTIONS - Compositions, devices, systems and methods for reducing and/or preventing photodamage of one or more reactants in illuminated analytical reactions by one or more of incorporating photodamage mitigating agents within the reaction mixture and/or interrogating different observation regions of the reaction mixture for a period that is less than a photodamage threshold period. | 02-02-2012 |
| 20120028251 | METHODS AND ARTICLES FOR DETECTING DEOXYRIBONUCLEASE ACTIVITY - The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms. | 02-02-2012 |
| 20120034601 | Porous Materials for Biological Sample Collection - Methods, apparatuses, and systems for collecting samples using hybrid porous materials that include an organic material and an inorganic material. A method for sample collection includes contacting a hybrid porous material and a biological sample to the porous material. The hybrid porous material includes an inorganic material and an organic material. The method includes placing the porous material with the attached sample in a liquid medium, wherein the sample is separated from the porous material in the liquid medium to form a separated sample, and collecting the separated sample in the medium. | 02-09-2012 |
| 20120034602 | Recombinant Polymerases For Improved Single Molecule Sequencing - Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template. | 02-09-2012 |
| 20120040338 | RBM3 in Testicular Cancer Diagnostics and Prognostics - The present disclosure provides a method for determining whether a mammalian subject belongs to a first or a second group, wherein subjects of the first group have a higher risk of having a testicular disorder than subjects of the second group, comprising the steps of: evaluating an amount of RBM3 protein or RBM3 mRNA in at least part of an earlier obtained sample comprising biological material from a testicle of said subject and determining a sample value corresponding to the evaluated amount; comparing said sample value with a predetermined reference value; and if said sample value is higher than said reference value, concluding that the subject belongs to the first group; and if said sample value is lower than or equal to said reference value, concluding that the subject belongs to the second group. Further, a prognostic method for testicular cancer is provided, as well as means and uses with prognostic and diagnostic applications. | 02-16-2012 |
| 20120040339 | Imaging Glyphosate in Plant Tissue - Methods and compositions are provided for spatial imaging and quantifying glyphosate in plant tissue. Glyphosate oxidoreductase is coupled to a cycling flavin mononucleotide-oxidoreductase-luciferase system. The resulting bioluminescence is proportional to the amount of glyphosate, allowing glyphosate to be observed within plant tissue and quantified. | 02-16-2012 |
| 20120040340 | NUCLEOTIDE ANALOGS - The invention provides for nucleotide analogs and methods of using the same, e.g., for sequencing nucleic acids. | 02-16-2012 |
| 20120058467 | APPARATUS AND METHOD FOR DETECTING DNA DAMAGE - The invention relates to an apparatus and an in-situ method for detecting cellular DNA damage. The invention is particularly suited for use in Comet assays and for automation of such assays. The apparatus comprises a multiwell plate, the plate comprising an array of wells held in fixed relationship with each other and each well having an axis, side walls, and a base and wherein the well is provided with electrode pairs disposed therein; and wherein there is provided means for parallel connection of the electrode pairs to an external voltage supply. | 03-08-2012 |
| 20120058468 | ADAPTORS FOR NUCLEIC ACID CONSTRUCTS IN TRANSMEMBRANE SEQUENCING - The invention relates to adaptors for sequencing nucleic acids. The adaptors may be used to generate single stranded constructs of nucleic acid for sequencing purposes. Such constructs may contain both strands from a double stranded deoxyribonucleic acid (DNA) or ribonucleic acid (RNA) template. The invention also relates to the constructs generated using the adaptors, methods of making the adaptors and constructs, as well as methods of sequencing double stranded nucleic acids. | 03-08-2012 |
| 20120058469 | FUNCTIONALIZED CYANINE DYES (PEG) - The invention provides a novel class of cyanine dyes that are functionalized with a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates. | 03-08-2012 |
| 20120058470 | ELECTRICAL WIRING OF POLYNUCLEOTIDES FOR NANOELECTRONIC APPLICATIONS - The present invention relates to incorporation and patterning of polynucleotide molecular wires onto surfaces. In one embodiment, two or more thiol-modified polynucleotide anchors are separately attached to metal contacts that are in turn separately attached to a substrate. Each polynucleotide anchor contains an unpaired region of bases that when bound to complimentary regions of a polynucleotide bridge molecule allow for electrical communication between contacts, and therefore detection of the polynucleotide bridge. | 03-08-2012 |
| 20120064516 | NUCLEIC ACID AMPLIFICTION REACTION APPARATUS, SUBSTRATE FOR NUCLEIC ACID AMPLIFICATION REACTION APPARATUS, AND NUCLEIC ACID AMPLIFICATION REACTION METHOD - A nucleic acid amplification reaction apparatus includes: a reaction region formed as a nucleic acid amplification reaction site in the shape of a tapered well of a decreasing horizontal cross sectional area along a light axis direction in which a substance that precipitates in the course of a nucleic acid amplification reaction settles; temperature controller that heats the reaction region; irradiator that shines light on the reaction region; and detector that detects the quantity of the light scattered out of the reaction region by the precipitated substance. | 03-15-2012 |
| 20120064517 | DETECTION OF CHROMATIN STRUCTURE - The present invention provides methods of determining the accessibility of genomic DNA to a DNA modifying agent. | 03-15-2012 |
| 20120064518 | Methods, tip assemblies and kits for introducing material into cells - Methods, tip assemblies and kits are provided for introducing material into cells. The tip assemblies include an attachment portion, a channel portion, and a constriction that function to reduce fluid pressure as a fluid passes through the constriction portion from the channel portion, whereby the tip assemblies form pores in the membranes of cells and introduce material into the cells. The material includes for example one selected from the group of: an inorganic compound, a drug, a genetic material, a protein, a carbohydrate, a synthetic polymer, and a pharmaceutical composition. | 03-15-2012 |
| 20120064519 | SYSTEM AND METHOD FOR DUAL-DETECTION OF A CELLULAR RESPONSE - A system and method as defined herein for dual-detection of evanescent-wave label-free light and evanescent-wave excited-fluorescent label-emitted light in an optical biosensor. | 03-15-2012 |
| 20120070823 | METHOD AND DEVICE FOR AUTOMATICALLY PROCESSING A SAMPLE - The invention relates to a method and a device for processing a biological sample. According to the invention, the processing comprises binding, washing and eluting biomolecules of the biological sample. The aim of the invention is to provide a method and a device for the automatic processing of biological samples which can have a relatively large volume and which can be further process by a microfluidic system. According to the invention, a container ( | 03-22-2012 |
| 20120070824 | THERMOSTABLE PROTEINASES FROM THERMOPHILIC BACTERIA - The present invention relates to the preparation of a method for the preparation of nucleic acid samples, including the steps of adding at least one non-specific thermophilic enzyme to a sample containing nucleic acid for testing, and incubating said sample for a preferred period at 65-80° C. as required to effect one or more of lysis of cells, digestion of proteins, digestion of cell-wall enzymes, via activity of said thermophilic enzyme, wherein said thermophilic enzyme is substantially stable and active at 65-80° C. but which is readily autolysed and/or denatured when said sample is incubated at or above 90° C. | 03-22-2012 |
| 20120070825 | Novel Compounds and Derivatizations of DNAs and RNAs on the Nucleobases of Pyrimidines for Function, Structure and Therapeutics - Disclosed are compounds of formula (I), a derivative, or a tautomer thereof, or a pharmaceutically acceptable salt of said compound or said tautomer. Also disclosed are methods of preparing compound of formula (I), a derivative, or a tautomer thereof, or a pharmaceutically acceptable salt of said compound or said tautomer. Further disclosed are methods of conducting drug discovery and research comprises applying the compound of formula (I), a derivative, or a tautomer thereof, or a pharmaceutically acceptable salt of said compound or said tautomer in an investigation. | 03-22-2012 |
| 20120077186 | USE OF CYSTEINE-DERIVED SUPPRESSOR TRNAS FOR NON-NATIVE AMINO ACID INCORPORATION - Disclosed herein are compositions and methods for incorporating non-native amino acids into a single polypeptide. In one example, an isolated modified suppressor tRNA is disclosed that includes the following: a modified tRNA | 03-29-2012 |
| 20120077187 | RECOMBINANT ANTIGEN FOR DETECTION OF TOXOCARIASIS - A vector comprising a polynucleotide sequence encoding a polypeptide having an amino acid sequence of SEQ ID NO: 2 for detecting antibody against | 03-29-2012 |
| 20120077188 | METHODS FOR MANIPULATING LIQUID SUBSTANCES IN MULTI-CHAMBERED RECEPTACLES - A receptacle having a plurality of interconnected chambers arranged to permit multiple process steps or processes to be performed independently or simultaneously. The receptacles are manufactured to separate liquid from dried reagents and to maintain the stability of the dried reagents. An immiscible liquid, such as an oil, is included to control loading of process materials, facilitate mixing and reconstitution of dried reagents, limit evaporation, control heating of reaction materials, concentrate solid support materials to prevent clogging of fluid connections, provide minimum volumes for fluid transfers, and to prevent process materials from sticking to chamber surfaces. The receptacles can be adapted for use in systems having a processing instrument that includes an actuator system for selectively moving fluid substances between chambers and a detector. The actuator system can be arranged to concentrate an analyte present in a sample. The detector can be used to detect an optical signal emitted by the contents of the receptacle. | 03-29-2012 |
| 20120077189 | SCAFFOLD-BASED POLYMERASE ENZYME SUBSTRATES - The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates. | 03-29-2012 |
| 20120077190 | SUBSTRATES, SYSTEMS AND METHODS FOR ANALYZING MATERIALS - Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses. | 03-29-2012 |
| 20120082975 | DNA-BASED MOLECULAR SWITCHES AND USES THEREOF - Disclosed are nucleic acid-based molecular switches that respond to changes in pH. The switches may be used in DNA nanodevices. The switches may also act as sensors for measuring the pH of a sample, including cells, regions thereof, and whole organisms. The switch includes an A-motif that forms at acidic pH. Also disclosed are compositions and methods for measuring the pH of cells or regions thereof, such as vesicles, the nucleus, mitochondrial matrix, or the Golgi lumen. | 04-05-2012 |
| 20120082976 | METHOD OF SCREENING FOR INSULIN SECRETION-POTENTIATING AGENTS - Disclosed are a novel method of screening for insulin secretion-potentiating agents as well as means for performing such screening. The means include a DNA encoding fluorescent-labeled Epac2 comprising two different DNAs encoding two different fluorescent proteins which emit fluorescent light with wavelength differing from each other and a DNA encoding Epac2 which are fused together in-frame, and the cells transformed with the DNA. Also disclosed is a method of screening insulin secretion-potentiating agents comprising bringing a candidate compound into contact with cells transformed with the said DNA, and detecting whether the compound binds to Epac2. | 04-05-2012 |
| 20120082977 | ARTICLES WITH MATRIX COMPRISING A CELL EXTRACTANT AND BIODETECTION METHODS THEREOF - Articles are provided for the detection of cells in a sample. The articles include a release element. The release element comprises an encapsulating agent and a cell extractant. The release element controls the release of the cell extractant into a liquid mixture containing the sample. Methods of use are also disclosed. | 04-05-2012 |
| 20120088232 | Aptamer-Based Device For Detection Of Cancer Markers And Methods Of Use - Systems and methods are provided for detecting and quantitating one or more compounds or molecules in a sample. An aptamer-based point-of-care device (such as a strip) is described for rapid detection of target molecules such as the cancer marker p-glycoprotein (Pgp). Fluorescent molecules or gold nanoparticles may be used to detect the binding between a target molecule and the aptamer. By way of example, fluorescence resonance energy transfer (FRET) or Dynamic Light Scattering (DLS) may be used for detecting the physical and/or chemical changes caused by the binding of the aptamers to the target molecules. | 04-12-2012 |
| 20120088233 | Method of Preparing Quality Control Material for FFPE - This specification relates to Formalin-fixed embedded quality control material for use for validation, verification, and to run controls for molecular assays. The quality control material can be used for a variety of tissues and for a variety of molecular assays. The quality control material can be used in commercial labs for validation and limit-of-detection analyses. | 04-12-2012 |
| 20120094278 | METHODS AND APPARATUS FOR CHARACTERIZING POLYNUCLEOTIDES - Systems and methods for analysis of polymers, e.g., polynucleotides, are provided. The systems are capable of analyzing a polymer at a specified rate. One such analysis system includes a structure having a nanopore aperture and a molecular motor, e.g., a polymerase, adjacent the nanopore aperture. | 04-19-2012 |
| 20120094279 | Use of enzymes for altering ratios of partially matched polynucleotides - The present disclosure relates to novel methods of discriminating and/or detecting mis-matched polynucleotide populations in a sample by determining the ratios of mismatched polynucleotide species after specific enzymatic digestion treatment. Aspects of this disclosure includes obtaining, enhancing and/or determining the amount of one DNA or RNA species versus another in a given sample following enzyme digestion treatment; determining the relative abundance of the species contained in the sample based on the changes in the relative ratios following enzymatic treatment. | 04-19-2012 |
| 20120094280 | DERIVATIZATION OF BIOMOLECULES BY COVALENT COUPLING OF NON-COFACTOR COMPOUNDS USING METHYLTRANSFERASES - The present invention relates to a use of non-cofactor compounds, represented by formulas (I) or (II) wherein R and Z are independently selected from H, D, C | 04-19-2012 |
| 20120094281 | ARTICLES WITH SHELL STRUCTURES INCLUDING A CELL EXTRACTANT AND BIODETECTION METHODS THEREOF - Articles are provided for the detection of cells in a sample. The articles include a release element comprising a cell extractant. The release element includes a shell structure that controls the release of the cell extractant into a liquid mixture containing the sample. Methods of use are also disclosed. | 04-19-2012 |
| 20120094282 | METHOD FOR DETECTING G-QUADRUPLEX, METHOD FOR DETECTING G-QUADRUPLEX-FORMING DNA AND METHOD FOR DETERMINING TELOMERASE ACTIVITY - A method is provided for specifically detecting a G-quadruplex, and the like. The method is characterized by including the steps of preparing a solution including an anionic planar phthalocyanine and mixing the solution with a sample solution to obtain a liquid mixture. The solution includes an anionic planar phthalocyanine. The method also includes a step of measuring the absorbance at 640 to 740 nm of the obtained liquid mixture. | 04-19-2012 |
| 20120100530 | ENZYME MUTANT - The invention relates to constructs comprising a nucleic acid binding protein and a surface. At least one native accessible cysteine residue is removed from the binding protein. The binding protein is attached to the surface via one or more accessible cysteine residues. The removal of other accessible cysteine residues from the protein allows control attachment to the surface. The constructs can be used to generate transmembrane pores having a nucleic acid binding protein attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect each of its component nucleotides by stochastic sensing. | 04-26-2012 |
| 20120100531 | COATED SUBSTRATES COMPRISING A CELL EXTRACTANT AND BIODETECTION METHODS THEREOF - Articles are provided for the detection of cells in a sample. The articles include a release element. The release element comprises a coated substrate that includes a cell extractant. The release element controls the release of the cell extractant into a liquid mixture containing the sample. Methods of use are also disclosed. | 04-26-2012 |
| 20120100532 | METHOD FOR IMAGING AND DIFFERENTIAL ANALYSIS OF CELLS - Provided are methods for determining and analyzing photometric and morphometric features of small objects, such as cells to, for example, identify different cell states. In particularly, methods are provided for identifying apoptotic cells, and for distinguishing between cells undergoing apoptosis versus necrosis. | 04-26-2012 |
| 20120100533 | METHOD FOR DETERMINING THE CARDIO-GENERATIVE POTENTIAL OF MAMMALIAN CELLS - This document is related to a method for determining the cardio-generative potential of mammalian cells which comprises the assessment of a CARdiac generation Potential Index (CARPI) as a function of the quantification of the expression of genes of said cells. It also relates to a method for quantitatively assessing the modification of this cardio-generative potential and the cardiogenic potential of a treatment aiming at cellular differentiation. | 04-26-2012 |
| 20120107799 | DISPOSABLE, RAPID EXTRACTION APPARATUS AND METHODS - An extraction apparatus and methods adapted to extract and preferably also isolate structures of interest from a test sample. The extraction apparatus contains a volume-dispensing mechanism that facilitates control over the injection of a sample suspected of containing a structure of interest into a filtration vessel that contains a membrane filter that associates with the structure of interest, preferably by adsorbing or binding thereto, and a collection container associated therewith. Methods of extracting, isolating, testing, and instructions regarding such methods are also included. | 05-03-2012 |
| 20120107800 | CELL-TARGETING NANOPARTICLES AND USES THEREOF - The invention generally refers to a cell-targeting nanoparticle, wherein the cell-targeting nanoparticle is directly conjugated to at least one cysteine-containing peptide, wherein the cysteine-containing peptide is selected from the group consisting of glutathione (GSM, a GSH-containing peptide, and a peptide comprising a nuclear localization signal (NLS) sequence and a transporter sequence, and methods of using the cell-targeting nanoparticle. | 05-03-2012 |
| 20120107801 | HIGH-EFFICIENCY HOMOLOGOUS RECOMBINATION IN THE OIL-PRODUCING ALGA, NANNOCHLOROPSIS - Transformation methods are provided for introducing deoxyribonucleic acid (DNA) into the nucleus of an algal cell. A transformation construct may be prepared, with the transformation construct having a first sequence of DNA similar to a corresponding first sequence of nuclear DNA, a second sequence of DNA similar to a corresponding second sequence of the nuclear DNA, and a sequence of DNA inserted between the first and second sequences of DNA of the transformation construct. A target sequence of DNA inserted between the first and second corresponding sequences of the nuclear DNA may be transformed, resulting result in replacement of the target sequence of DNA with the sequence of DNA of interest. | 05-03-2012 |
| 20120107802 | METHOD FOR SEQUENCING A HETEROPOLYMERIC TARGET NUCLEIC ACID SEQUENCE - The invention relates to a method for sequencing a heteropolymeric target nucleic acid sequence that involves stochastic sensing. The invention also relates to a method for improving a pore for sequencing a target nucleic acid sequence by modifying one or more sites in the pore. | 05-03-2012 |
| 20120107803 | METHOD FOR ENUMERATION OF MAMMALIAN MICRONUCLEATED ERYTHROCYTE POPULATIONS, WHILE DISTINGUISHING PLATELETS AND/OR PLATELET-ASSOCIATED AGGREGATES - A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures. | 05-03-2012 |
| 20120107804 | DISINTEGRATION OF CELLULAR COMPONENTS IN BODY FLUIDS - The present invention refers to a method of processing a biological fluid which comprises cellular components by a freezing/thawing treatment. The method is particularly useful for preparing biological samples for analyte detection. | 05-03-2012 |
| 20120107805 | Generating a Fluid Stream in a Microfluidic Device - A fluid handling and delivery system useful in generating a fluid stream in the flow path of microfluidic device. | 05-03-2012 |
| 20120107806 | METHOD AND KITS FOR REPAIRING NUCLEIC ACID SEQUENCES - Methods and kits for DNA repair are provided. The methods and kits described herein repair multiple types of DNA damage. The kit may include a plurality of enzymes to repair a greater variety of lesions than any single enzyme is capable of repairing. Repair of damaged DNA may include releasing damaged bases from the DNA strand, nicking the DNA at the damaged sites, translating the nicks via 5′-3′ exonuclease activity, and sealing the nicks. The enzymes employed in the repair process may then be heat-inactivated, thereby obviating a purification process. The repaired DNA may then be analyzed using a variety of DNA analysis methods. | 05-03-2012 |
| 20120115127 | MOLECULAR FLUX RATES THROUGH CRITICAL PATHWAYS MEASURED BY STABLE ISOTOPE LABELING IN VIVO, AS BIOMARKERS OF DRUG ACTION AND DISEASE ACTIVITY - The methods described herein enable the evaluation of compounds on subjects to assess their therapeutic efficacy or toxic effects. The target of analysis is the underlying biochemical process or processes (i.e., metabolic process) thought to be involved in disease pathogenesis. Molecular flux rates within the one or more biochemical processes serve as biomarkers and are quantitated and compared with the molecular flux rates (i.e., biomarker) from control subjects (i.e., subjects not exposed to the compounds). Any change in the biomarker in the subject relative to the biomarker in the control subject provides information to evaluate therapeutic efficacy of an administered drug or a toxic effect and to develop the compound further if desired. In one aspect of the invention, stable isotope-labeled substrate molecules are administered to a subject and the label is incorporated into targeted molecules in a manner that reveals molecular flux rates through metabolic pathways of interest. | 05-10-2012 |
| 20120115128 | SELECTIVE PROTEIN LABELING - The present invention is related to methods of detecting protein-protein interactions in living cells, as well as detecting the formation and/or inhibition of protein-protein interactions in cells. | 05-10-2012 |
| 20120115129 | ENZYME SUBSTRATE COMPRISING A FUNCTIONAL DYE AND ASSOCIATED TECHNOLOGY AND METHODS - Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease. | 05-10-2012 |
| 20120115130 | METHOD FOR DETECTING TARGET CELL - A target cell detection method includes performing a test measurement on a dispersion to obtain a measurement result 1, the test measurement being an optical or electromagnetic measurement, and the dispersion including labeled particles and target cells, the labeled particles being particles on each of which a substance that specifically binds to a specific molecule present on a surface of each of the target cells is immobilized, performing measurement that is identical with the test measurement on a dispersion that includes the target cells, but does not include the labeled particles to obtain a measurement result 2, and comparing the measurement result 1 with the measurement result 2. | 05-10-2012 |
| 20120122083 | EXPRESSION VECTOR FOR PRODUCING PROTEIN DERIVED FROM FOREIGN GENE IN LARGE QUANTITY USING ANIMAL CELLS, AND USE THEREOF - The present inventors conducted dedicated studies and successfully constructed expression vectors that enable high-level production of foreign gene-derived proteins in mammalian host cells, which comprise a translation-impaired dihydrofolate reductase gene cistron whose expression has been attenuated by altering the codons to the least frequently used codons in mammals; and a gene cassette which has a cloning site for incorporation of a foreign gene between a highly transcriptionally active promoter and a highly stable polyadenylation signal. | 05-17-2012 |
| 20120122084 | SYSTEM FOR IDENTIFYING AND SORTING LIVING CELLS - In embodiments of the present invention, a system and method of cytometry may include presenting a single sperm cell to at least one laser source configured to deliver light to the sperm cell in order to induce bond vibrations in the sperm cell DNA, and detecting the signature of the bond vibrations. The bond vibration signature is used to calculate a DNA content carried by the sperm cell which is used to identify the sperm cell as carrying an X-chromosome or Y-chromosome. Another system and method may include flowing cells past at least one QCL source one-by-one using a fluid handling system, delivering QCL light to a single cell to induce resonant mid-IR absorption by one or more analytes of the cell, and detecting, using a mid-infrared detection facility, the transmitted mid-infrared wavelength light, wherein the transmitted mid-infrared wavelength light is used to identify a cell characteristic. | 05-17-2012 |
| 20120122085 | RECQL4/RECQL4 VARIANT-P53 COMPLEX FOR ALTERED MITOCHONDRIAL FUNCTION IN ROTHMUND-THOMSON SYNDROME - The present invention discloses a functional interaction of the p53 with RECQL4/RECQL4 variant. The present invention further discloses co localization of RECQL4/RECQL4 variant and p53 complex in the mitochondrial nucleoids. The MLS at the N-terminus of RECQL4 that binds to Tom 20 receptor complex and transport RECQL4/RECQL4 variant-p53 complex into the mitochondria has been identified. Further the invention discloses measurement of intracellular ROS, identification of mtDNA mutations as a diagnostic tool for RTS and treatment with ascorbic acid for scavenging ROS and as treatment for RTS. | 05-17-2012 |
| 20120129158 | SYSTEMS AND METHODS FOR IDENTIFYING AND DISRUPTING CELLULAR ORGANELLES - This invention relates to optomechanical systems and methods for altering, modifying or disrupting a target object. Such systems and methods are used for, for example, ablating the endogenous nucleus in a cell. | 05-24-2012 |
| 20120129159 | METHODS AND COMPOSITIONS FOR PROTEIN LABELING USING LIPOIC ACID LIGASES - The present disclosure provides compositions and methods of use thereof for labeling peptide and proteins in vitro or in vivo. The methods described herein employ lipoic acid ligase or mutants thereof, and lipoic acid analogs (e.g., lipoic acid analogs comprising a resorufin moiety) recognized by lipoic acid ligase and lipoic acid ligase mutants. Also provided herein is a method of imaging protein-protein interaction via a reaction mediated by lipoic acid ligase. | 05-24-2012 |
| 20120129160 | RAPID IN VIVO GENE MUTATION ASSAY BASED ON THE PIG-A GENE - The invention relates to methods and kits for the quantitative analysis of in vivo mutation frequencies of the Pig-A gene in individuals, particularly using peripheral blood samples of vertebrates. | 05-24-2012 |
| 20120129161 | METHODS FOR MODULATING EMBRYONIC STEM CELL DIFFERENTIATION - Described herein is Zscan4, a gene exhibiting 2-cell embryonic stage and embryonic stem cell specific expression. Identification of nine Zscan4 co-expressed genes is also described. Inhibition of Zscan4 expression inhibits the 2-cell to 4-cell embryonic transition and prevents blastocyst implantation, expansion and outgrowth. Provided herein are methods of inhibiting differentiation of a stem cell, promoting blastocyst outgrowth of embryonic stem cells and identifying a subpopulation of stem cells expressing Zscan4. Further described is the identification of Trim43 as a gene exhibiting morula-specific expression. Also provided are isolated expression vectors comprising a Zscan4 promoter, or a Trim43 promoter operably linked to a heterologous polypeptide and uses thereof. Further provided are transgenic animals comprising transgenes encoding marker proteins operably linked to Zscan4 and Trim43 promoters. | 05-24-2012 |
| 20120129162 | CELL CULTURE SYSTEM FOR DETERMINING THE SENSITIZING, ALLERGENIC AND/OR IRRITATING EFFECT OF A SUBSTANCE - The present invention refers to a cell culture system especially for investigating the sensitizing, allergenic and/or irritating effect of substances, comprising a first and a second compartment that can communicate with each other via a permeable interlayer, whereby the first compartment has an epidermis model and the second a cell culture based on immune cells. | 05-24-2012 |
| 20120129163 | TESTING LUMENECTOMY SAMPLES FOR MARKERS OF NON-VASCULAR DISEASES - Lumenectomy material is tested to determine the presence or likelihood of a condition of a patient. The lumenectomy material is in the form of at least one continuous tissue strand collected in vivo from an inner surface of a body lumen of the patient. The presence of at least one marker of a disease is determined. The disease may be hypertension, hyperlipidemia, depression, obesity, metabolic syndrome, insulin resistance, kidney damage, or diabetes. The patient is identified as having or as likely to develop the disease if a marker of the disease is identified in the lumenectomy material of the patient. | 05-24-2012 |
| 20120129164 | DEVICE AND PROCESS FOR ISOLATING AND CULTIVATING LIVE CELLS ON A FILTER OR EXTRACTING THEIR GENETIC MATERIAL - The process for isolating live cells on a filter or extracting their genetic material. The process comprises the steps of attaching, at least temporarily, a filter to a lower opening of a compartment having, in addition, an air inlet; inserting into the compartment a liquid carrying the cells; and attaching, in an impermeable manner, a needle, at least temporarily, to the compartment opening, the filter being positioned between the needle and the interior volume of the compartment. The process further comprises the steps of perforation, with the needle, of a plug of a vacuum tube with negative pressure relative to ambient pressure; and aspiration, by means of negative pressure from the vacuum tube, of the liquid through the filter, the filter retaining the cells. | 05-24-2012 |
| 20120135399 | CANCER BIOMARKER AND METHODS OF USING THEREOF - Described herein are biomarkers which can be used for identifying a subject at risk for or evaluating the progression of cancer. In certain aspects, these biomarkers can be used to identify cancer stem cells. These biomarkers can include, but are not limited to, Oc1 or molecular variants thereof, Oc1 target proteins, or a combination thereof. In addition, described herein are methods for reducing the expression of these biomarkers associated with cancer. | 05-31-2012 |
| 20120135400 | Novel Fluorescent and Colored Proteins, and Polynucleotides that Encode These Proteins - The subject invention provides new fluorescent and/or colored proteins, and polynucleotide sequences that encode these proteins. The subject invention further provides materials and methods useful for expressing these detectable proteins in biological systems. | 05-31-2012 |
| 20120141981 | SECOND HARMONIC IMAGING NANOPROBES AND TECHNIQUES FOR USE THEREOF - Second harmonic nanoprobes for imaging biological samples and a method of using such probes to monitor the dynamics of biological process using a field resonance enhanced second harmonic (FRESH) technique are provided. The second harmonic generating (SHG) nanoprobes are comprised of various kinds of nanocrystals that do not possess an inversion symmetry and therefore are capable of generating second harmonic signals that can then be detected by conventional two-photon microscopy for in vivo imaging of biological processes and structures such as cell signaling, neuroimaging, protein conformation probing, DNA conformation probing, gene transcription, virus infection and replication in cells, protein dynamics, tumor imaging and cancer therapy evaluation and diagnosis as well as quantification in optical imaging. | 06-07-2012 |
| 20120141982 | Culture Method for Obtaining a Clonal Population of Antigen-Specific B Cells - The present invention relates to methods of isolating antigen-specific cells and producing antibodies therefrom. | 06-07-2012 |
| 20120141983 | Quantitative Aggregation Sensors - Composition, systems and methods are provided for quantifying the amount of protein aggregation occurring in a cell in vitro and in vivo. These compositions, systems and methods find use in a number of applications, including in screening candidate agents for activity in modulating intracellular and extracellular protein aggregation in vitro and in vivo; in the generation of in vivo data for modeling aggregation processes in the cellular environment; for the validation of in vitro data, e.g. the effect of point mutations on the aggregation of amyloidogenic proteins; for the proteomic analysis of interacting partners so as to identify new therapeutic targets; for the analysis of changes in gene expression that are induced by intracellular versus extracellular aggregation; and for the evaluation of changes in the activity of the cellular network that controls protein folding and aggregation, the so-called proteostasis network. | 06-07-2012 |
| 20120141984 | METHODS OF ISOLATING STEM CELLS - The present inventors discovered for the first time that labeling cell nuclei makes it possible to efficiently isolate stem cells. Namely, it was elucidated that stem cells with labeled nuclei remained labeled even after cell division, and showed self-renewing and long-living abilities characteristic of stem cells. Efficient isolation of stem cells is possible, for instance, by labeling the nuclear of each cell in a heterogeneous cellular group followed by selecting those cells that maintain a labeled state even after cell division. The present invention provides methods for enabling visualization of stem cells of animal tissues in a living state by labeling using the essential functions of the stem cells, and methods for simply and easily isolating the stem cells in a fresh state without using at all genetic manipulation or artificial markers. | 06-07-2012 |
| 20120141985 | REAL-TIME MONITORING OF DEPLETION OF HIGH-ABUNDANCE BLOOD PROTEINS OR RECOVERY OF LOW-ABUNDANCE BLOOD PROTEINS BY UV SPECTROMETRY - Disclosed is a method for monitoring depletion of high-abundance and/or recovery of low-abundance proteins from blood in real time, comprising: (a) labeling high-abundance and/or low-abundance proteins of a blood specimen with a fluorescent or UV marker; and (b) passing blood samples containing the fluorescent or UV marker-labeled high-abundance and/or low-abundance proteins through a removal column. | 06-07-2012 |
| 20120156671 | LABELLED NUCLEOTIDES - The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula (1) (wherein X is selected from the group comprising O, S, NH and NQ wherein Q is a C | 06-21-2012 |
| 20120156672 | NOVEL STRINGENT SELECTABLE MARKERS - The present invention relates to nucleic acid constructs comprising selectable marker genes in a multicistronic transcription unit for use in the generation and selection of eukaryotic host cells for expression of a gene product of interest. For increased stringency of selection, the coding sequence of the selectable marker may be directed preceded by a relatively short functional open reading frame to reduce the efficiency of translation of the selectable marker, and/or the amino acid sequence of the selectable marker may comprise one or more mutations that reduce the level of resistance provide by the mutated marker as compared to its wild type counterpart. The invention further relates to methods for generating eukaryotic host cells for expression of a gene product of interest, wherein these nucleic acid constructs are used, and to methods for producing a gene product of interest wherein thus generated host cells are applied. | 06-21-2012 |
| 20120156673 | METHODS FOR AGROBACTERIUM-MEDIATED TRANSFORMATION OF SUGAR CANE - The present invention provides methods for producing a transformed sugar cane tissue or cell thereof, said methods comprising: a) inoculating a sugar cane tissue or a cell thereof with an | 06-21-2012 |
| 20120164630 | METHODS FOR MAINTAINING THE INTEGRITY AND IDENTIFICATION OF A NUCLEIC ACID TEMPLATE IN A MULTIPLEX SEQUENCING REACTION - The invention generally relates to methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction. In certain embodiments, methods of the invention involve obtaining a template nucleic acid, incorporating a pair of sequence identifiers into the template, and sequencing the template. | 06-28-2012 |
| 20120164631 | APPARATUS AND METHOD FOR PERFORMING NUCLEIC ACID ANALYSIS - The present invention relates to optical confinements, methods of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis. | 06-28-2012 |
| 20120164632 | QUANTITATION OF CELLULAR DNA AND CELL NUMBERS USING ELEMENT LABELING - Methods and kits for the quantitation of cellular DNA and cell numbers are provided. Passive element uptake, element-labeled DNA intercalators, and element labeled affinity reagents are used to quantify DNA and cells. The DNA and the cells are analyzed by elemental analysis, including ICP-MS. The methods and kits provide a fast and accurate analysis of cellular DNA and cell numbers. | 06-28-2012 |
| 20120164633 | DIGITAL DROPLET SEQUENCING - The present invention provides systems, devices, methods, kits, and compositions for sorting and analysis of nucleic acid sequences using digital droplet PCR. In particular, provided herein are methods to convert complex samples into a plurality of simplified samples, and sequence analysis thereof. | 06-28-2012 |
| 20120164634 | Method for Separating Particles and/or Cells Having 2 and More Surface Specificities - The invention describes an easy method for the gentle separation of cells and/or particles from liquids, preferably blood. For that purpose known steps of magnetic separation procedures are combined with capture particle-assisted separating methods. | 06-28-2012 |
| 20120164635 | LARGE STOKE SHIFT NIR DYES - A compound of the following formula: | 06-28-2012 |
| 20120164636 | MAMMALIAN OOCYTE DEVELOPMENT COMPETENCY GRANULOSA MARKERS AND USES THEREOF - The present invention relates to the competence of oocytes to uterine implantation and development into living individual. The invention more particularly relates to marker that are detected and measured in granulosa cells collected along with the oocytes during oocyte aspiration as it is done in assisted reproduction techniques. | 06-28-2012 |
| 20120171665 | FLUORESCENT MARKERS AND USE THEREOF FOR LABELING SPECIFIC PROTEIN TARGETS - Novel fluorescent markers of Formula I: | 07-05-2012 |
| 20120171666 | Rare Cell Analysis Using Sample Splitting And DNA Tags - The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample. | 07-05-2012 |
| 20120171667 | Rare Cell Analysis Using Sample Splitting And DNA Tags - The present invention provides systems, apparatuses, and methods to detect the presence of fetal cells when mixed with a population of maternal cells in a sample and to test fetal abnormalities, e.g. aneuploidy. The present invention involves labeling regions of genomic DNA in each cell in said mixed sample with different labels wherein each label is specific to each cell and quantifying the labeled regions of genomic DNA from each cell in the mixed sample. More particularly the invention involves quantifying labeled DNA polymorphisms from each cell in the mixed sample. | 07-05-2012 |
| 20120171668 | ENHANCED DEPOSITION OF CHROMOGENS UTILIZING PYRIMIDINE ANALOGS - This disclosure relates to compositions that enhance the deposition of detectable moieties on tissue samples, methods utilizing these compositions and kits including these compositions. The compositions include a deposition enhancer having a formula | 07-05-2012 |
| 20120178080 | 1,2,4-OXADIAZOLE BENZOIC ACID COMPOSITIONS AND THEIR USE IN BIOASSAYS - Novel 1,2,4-oxadiazole benzoic acid compounds, methods of using and pharmaceutical compositions comprising an 1,2,4-oxadiazole benzoic acid derivative are disclosed. The methods include methods of treating or preventing a disease ameliorated by modulation of premature translation termination or nonsense-mediated mRNA decay, or ameliorating one or more symptoms associated therewith. | 07-12-2012 |
| 20120183953 | GENOME ASSEMBLY - The invention generally relates to methods for assembling sequence contigs. In certain embodiments, methods of the invention involve converting sequence contigs into maps, generating a plurality of single molecule restriction maps, aligning single molecule restriction maps to ends of the maps of the sequence contigs, thereby producing extended sequence contigs, and aligning extended sequence contigs. | 07-19-2012 |
| 20120183954 | LUMINESCENT DYES WITH A WATER-SOLUBLE INTRAMOLECULAR BRIDGE AND THEIR BIOLOGICAL CONJUGATES - Chemically reactive dyes that are intramolecularly crosslinked with a water-soluble bridge, their bioconjugates and their uses are described. Reactive fluorescent dyes that have a water-soluble bridge are superior to those of conjugates of spectrally non-crosslinked dyes or the dyes that are crosslinked with a hydrophobic bridge. The invention includes reactive fluorescent dyes, their biological conjugates and uses. | 07-19-2012 |
| 20120183955 | Design and construction of Bifunctional Short Hairpin RNA - A method for designing a bi-shRNA expression cassette encoding a bi-shRNA comprising: selecting one or more target site sequences; providing a backbone sequence comprising a first and a second stem-loop structure, inserting a first passenger strand and a second passenger strand and providing for synthesis of the bi-shRNA expression cassette. | 07-19-2012 |
| 20120183956 | SAMPLE HANDLING - The invention provides containers and methods of use for the storage, transportation and preparation of samples, such as DNA samples for analysis. The container is pre-provided with the reagents in sealed chambers. The sample can be introduced and the container manipulated to release the reagents, provide the necessary conditions and give a fully prepared sample. The container can then be engaged with an analysis device to identify characteristics of the sample or perform other operations thereon. | 07-19-2012 |
| 20120183957 | SYSTEMS AND METHODS FOR MEASURING TRANSLATION OF TARGET PROTEINS IN CELLS - The present invention relates to systems and methods for measuring the rate of translation of a target protein in cells, which are based on the detection of translation of one or more predetermined codon pairs during synthesis of the target protein. The detection is provided by a FRET signal emitted from labeled tRNA molecules which are juxtaposed during synthesis of the protein. | 07-19-2012 |
| 20120190012 | COMPOSITIONS AND METHODS FOR DNA SEQUENCING - The invention provides compositions and methods useful in DNA sequencing. In exemplary embodiments, a detectable label such as a luminescent macrocycle is used. | 07-26-2012 |
| 20120190013 | HYDROLASE ENZYME SUBSTRATES AND USES THEREOF - The present invention provides novel methods for determining the presence or amount of a hydrolytic enzyme in a sample, based on novel substrates for the enzymes, and also provides compositions and methods that provide highly sensitive assay methods for such hydrolytic enzymes. | 07-26-2012 |
| 20120190014 | PHAGE PHI 29 DNA POLYMERASE CHIMERA - A DNA polymerase chimera comprising an amino-terminal (N-terminal) region encoding a φ29 type DNA polymerase and a carboxyl-terminal (C-terminal) region comprising at least one HhH domain which are bound by means of a connecting amino acid sequence is disclosed along with and the use thereof for replicating, amplifying or sequencing a template DNA. Also disclosed is a method for replicating, amplifying or sequencing a deoxyribonucleic acid with said DNA polymerase chimera and kits for carrying out said methods. | 07-26-2012 |
| 20120190015 | METHOD FOR DETERMINING THE PRESENCE AND CONCENTRATION OF ANALYTES USING A NUCLEIC ACID LIGAND AND RARE EARTH ELEMENTS - The present invention relates to methods and an apparatus for determining the presence and concentration of an analyte in a sample and the binding of the analyte to a nucleic acid ligand that include measuring the fluorescence emitted by a rare earth element, i.e., terbium, in the presence of the analyte and the nucleic acid ligand. Specific embodiments include the use of terbium and nucleic acid ligands that specifically bind the mycotoxin ochratoxin. A, to detect and quantify ochratoxin A in, for example, food samples such as grain, wine, or beer. The detection of thrombin using terbium and a thrombin-specific nucleic acid ligand is also disclosed. The present invention also relates to a composition comprising a rare earth element as a cation that facilitates the binding of an analyte to a nucleic acid ligand of the analyte. | 07-26-2012 |
| 20120196278 | RNA POLYPHOSPHATASE COMPOSITIONS, KITS, AND USES THEREOF - The present invention relates to the discovery of RNA 5′ polyphosphatase enzymes not previously described in the art, methods for discovery of said enzymes, compositions of said enzymes, methods for making said enzymes, and various methods and kits for using said enzymes for biomedical research, for human and non-human diagnostics, for production of therapeutic products, and for other applications. In particular, some embodiments provide compositions, kits and methods for employing RNA polyphosphatases for isolation, purification, production, and assay of capped RNA using a biological sample or a sample from an in vitro capping reaction wherein the sample also contains RNA that is not capped. Other embodiments provide compositions, kits and methods wherein RNA polyphosphatases comprise signal-amplifying enzymes for analyte-specific assays. | 08-02-2012 |
| 20120196279 | METHODS AND COMPOSITIONS FOR NUCLEIC ACID SAMPLE PREPARATION - Provided are methods and compositions for the production of double-stranded nucleic acids, which can optionally be used as templates in high-throughput sequencing systems. In certain embodiments, these templates do not require exogenous primers to facilitate initiation of polymerase-dependent nascent strand synthesis. In certain embodiments, these templates comprise a single-stranded or gapped region that serves as a polymerase priming site. | 08-02-2012 |
| 20120196280 | MICROFABRICATED DEVICE FOR METERING AN ANALYTE - The present invention relates to a microfabricated device for metering an analyte comprising a nucleic acid sequence into a plurality of parallel reaction chambers for nucleic acid sequence amplification. The present invention further provides a method of metering an analyte into a plurality of parallel reaction units of an integrated microfabricated device. | 08-02-2012 |
| 20120196281 | FUNCTIONALIZED ORGANOTYPIC SYSTEMS - The present invention provides an organotypic system comprising a portion of a retina containing live cells in a cultured in medium, wherein photosensitivity has been restored in at least some of said live cells. Methods of use thereof are also provided. | 08-02-2012 |
| 20120202195 | NUCLEIC ACID ELEMENT FOR USE IN ANALYSIS, AND ANALYTICAL METHOD, ANALYTICAL REAGENT, AND ANALYTICAL INSTRUMENT USING SAME - The technique by which simple analysis of an intended subject to be analyzed can be carried out is provided. In this technique, a nucleic acid element | 08-09-2012 |
| 20120202196 | LABELLED NUCLEOTIDES - Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group. | 08-09-2012 |
| 20120202197 | EFFICIENT PROLIFERATION METHOD FOR A KUPFFER CELL AND USE THEREOF - The present invention has successfully established a mixed culture system capable of actively proliferating a Kupffer cell in a primary culture of a cell population derived from a liver. Additionally, the present invention has successfully established a novel production method for efficiently producing a large amount of highly purified Kupffer cells using this mixed culture system. | 08-09-2012 |
| 20120208181 | Biosynthesis Of Human Milk Oligosaccharides In Engineered Bacteria - The invention provides compositions and methods for engineering bacteria to produce fucosylated oligosaccharides, and the use thereof in the prevention or treatment of infection. | 08-16-2012 |
| 20120214156 | DEVICE INCLUDING A DISSOLVABLE STRUCTURE FOR FLOW CONTROL - A diagnostic device is provided that includes a plurality of retainment regions, with the retainment regions that are separated by at least one dissolvable barrier. The retainment regions can be interconnected through at least one fluid processing passageway. A retainment region can include a container such as a retainment region, well, chamber, or other receptacle, or a retainment region such as a surface on which the material is retained. The retainment regions can include a reaction retainment region, one or more reagent retainment regions, each containing unreacted reagents, and a sample retainment region. A pressure-actuated valve can be positioned in each fluid processing passageway interconnecting the one or more reagent retainment regions with the respective intermediate retainment regions interposed between each of the one or more reagent retainment regions and the reaction retainment region. The dissolvable barrier can be a fluid flow modulator in the at least one fluid processing passageway. | 08-23-2012 |
| 20120214157 | METHOD TO GENERATE OR DETERMINE NUCLEIC ACID TAGS CORRESPONDING TO THE TERMINAL ENDS OF DNA MOLECULES USING SEQUENCES ANALYSIS OF GENE EXPRESSION (TERMINAL SAGE) - We describe a method of providing an indication of an instance of expression of a gene, the method comprising the steps of: (a) providing a complementary deoxyribonucleic acid (cDNA) having a terminus comprising a terminal transcribed sequence of a gene; (b) linking the cDNA to an linker sequence thereby forming a linked nucleic acid, in which the linker sequence comprises a first recognition site for a first nucleic acid cleavage enzyme, preferably a restriction endonuclease, that allows nucleic acid cleavage at a site distant from the first recognition site; and (c) cleaving the linked nucleic acid with the first nucleic acid cleavage enzyme to provide a linked tag, in which the linked tag comprises a nucleotide sequence tag representative of a terminal transcribed sequence of the gene; and (d) detecting the presence or identity of the linked tag or the nucleotide sequence tag to provide an indication of an instance of gene expression. | 08-23-2012 |
| 20120231447 | Surface Passivation Methods for Single Molecule Imaging of Biochemical Reactions - The present disclosure provides methods for treating a surface for single-molecule imaging. | 09-13-2012 |
| 20120231448 | LOW CELL TOXICITY ANTIBIOTIC HYGROMYCIN B - A preparation of antibiotic hygromycin B with low cell toxicity and high purity, and methods of preparing such a preparation, are provided. More specifically, an isolated antibiotic hygromycin B with a purity of greater than 98% and impurities C, D and E individually less than 0.5% and impurity F less than 2%, as measured by HPLC, is described. Uses of this high purity antibiotic hygromycin B include, for example, for in vitro cell selection. | 09-13-2012 |
| 20120231449 | PRODUCTS AND METHODS FOR ENHANCED TRANSGENE EXPRESSION AND PROCESSING - Disclosed are methods and eukaryotic host cells for transgene expression. The cells may be treated and/or modified to increase homologous recombination (HR), decrease non homologous end joining (NHEJ) and/or to enhance a HR/NHEJ ratio in said cell. Such cells can be transfected with vectors comprising the transgene, which advantageously integrates into the genome of the cell to form a concatemeric structure which may comprise more than 200 transgene copies. Certain expression enhancing elements such as MARs are advantageously provided to further enhance and/or facilitate transgene expression. Disclosed is also a recombinant eukaryotic host cell, in particular a non-primate host cell, comprising a transgenic sequence encoding a protein and/or a RNA, in particular a primate protein and/or RNA, involved in translocation across the ER membrane and/or secretion across the cytoplasmic membrane. | 09-13-2012 |
| 20120231450 | NOVEL PACEMAKER CELL - The present invention provides a pacemaker cell which possesses HCN4 channel and Na channel, the beating rate of which can be controlled by regulation of Na channel, wherein the cell is derived from embryonic stem (ES) cells, induced pluripotent stem (iPS) cells, or primordial germ cell-derived versatile cells. The present invention also provides a cardiac pacemaker comprising the pacemaker cell. | 09-13-2012 |
| 20120231451 | Induction of Germ Cells from Pluripotent Cells - Methods and compositions are provided for promoting germ cell differentiation from pluripotent cells, and for identifying agents that modulate germ cell differentiation. | 09-13-2012 |
| 20120237924 | Method to prevent missing PCR template and/or reaction mixture and its further use of preventing missing solution(s) in experiment - This invention discloses a method by colored solution to prevent missing a solution in experiment. | 09-20-2012 |
| 20120237925 | Portable Sample Disruptor Apparatus, Kits, and Methods - Apparatuses, kits, and methods for portable sample disruption (e.g., for encouraging cell lysis). | 09-20-2012 |
| 20120244525 | Oligonucleotide Adapters: Compositions and Methods of Use - Compositions are provided that include a synthetic oligonucleotide characterized by a double-stranded region, a single-stranded region, a forward primer site, a reverse primer site and one or more cleavage sites therebetween. Methods of use for these compositions include adapters for the amplification of DNA fragments. | 09-27-2012 |
| 20120244526 | IDENTIFICATION OF TISSUE FOR DEBRIDEMENT - Provided are methods of determining whether a cell in a tissue site is viable or nonviable. Also provided are methods of debriding tissue from a tissue site. Further provided are kits comprising a compound that distinguishes between viable and nonviable cells and instructions for using the compound on a tissue site. Additionally, the use of a compound that distinguishes between viable and nonviable cells is provided, where the use is to determine whether a cell in a tissue site is viable or nonviable. Also provided is a use of a compound that distinguishes between viable and nonviable cells, where the use is for the manufacture of the above-described kit. | 09-27-2012 |
| 20120252008 | COMPOSITIONS AND METHODS FOR CAPTURE AND ELUTION OF BIOLOGICAL MATERIALS VIA PARTICULATES - Lysing may include agitating a specimen in a chamber along with a medium that includes a particulate lysing material that has an affinity for a biological material. Lysing material may include beads or other material which may be coated that facilitates binding. The medium may include a fluid with a high salt or low pH level. Binding of biological materials to solid surfaces may be induced by particular media. The biological material may be eluted by lowering a concentration of salt or increasing a pH level. Lysing materials with two or more different affinities may be employed. Lysing materials may include particles of different sizes. Heating may be used. Lysing may be performed in a flow through apparatus. Surfaces of solid materials may be modified to capture bacteria with high cell wall lipid content. | 10-04-2012 |
| 20120252009 | Methods and Compositions for Enriching Either Target Polynucleotides or Non-Target Polynucleotides from a Mixture of Target and Non-Target Polynucleotides - Compositions and methods are provided for enriching non-target polynucleotides from a mixture of non-target and target polynucleotides where differences between the target polynucleotides and the non-target polynucleotides include the extent of modified bases that are present in a greater density in the target polynucleotides than in the non-target polynucleotides. This permits the target polynucleotides to be selectively and rapidly bound to an affinity matrix such as affinity protein-coated magnetic beads providing enrichment of the non-target polynucleotides in the supernatant. One use of this enrichment is to remove human genomic DNA from a mixture of DNAs obtained from human tissue samples to enrich for polynucleotides in a microbiome so as to characterize the microbiome by DNA sequencing. | 10-04-2012 |
| 20120252010 | LABELLED NUCLEOTIDES - Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group. | 10-04-2012 |
| 20120252011 | COMPOSITION FOR TREATMENT OF PANCREATIC CANCER - Disclosed is a composition for treating pancreatic cancer. The composition comprises a pharmaceutically effective amount of an antisense nucleic acid or siRNA that inhibits expression of at least one gene selected from the group consisting of SON gene, MCM5 gene, WDR5 gene, PBK gene and CENPA gene. The composition inhibits the expression of a specific gene to provide the effect of inhibiting the proliferation, survival and tumorigenicity of pancreatic cancer cells. | 10-04-2012 |
| 20120258449 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 10-11-2012 |
| 20120258450 | Method for Monitoring Gene Expression of Translation and Integral/Secretory Protein Synthesis by Magnetic Resonance Spectroscopy (MRS) - Disclosed is a method for monitoring change in gene expression of translation and integral/secretory protein synthesis in a sample comprising measuring the amount of Choline and/or a fatty acid present in the sample as a function of time wherein the measurement is carried out by Magnetic Resonance Spectroscopy (MRS) and correlating the amount of Choline and/or fatty acid measured as function of time to the change in gene expression of translation and integral/secretory protein synthesis in the sample. The level of Choline from organelles matches the level of Choline from transcription and integral/secretory protein synthesis. Quantification of translation and integral/secretory protein synthesis leads to quantification of genetic expression. During apoptosis the amount of Choline decreases, and the amount of fatty acids increase from distinct trafficking of Choline polar heads and fatty and tails of degraded endomembranes. | 10-11-2012 |
| 20120258451 | LASER ISOLATION OF VIABLE CELLS - Methods for laser microdissection isolation of viable cells are provided. Cells of a desired type may be isolated from a diverse population, optionally with detection and exclusion of undesired cells. Desired cells may be isolated from a population that arose from differentiation of pluripotent cells, preferably embryonic stem cells or induced pluripotent stem cells, and undifferentiated stem cells may be detected and excluded from selection including the isolation of RPE cells sleeted based on morphology (e.g., characteristic mottled appearance) from a population of ES cells. The cells isolated by these methods, including RPE cells, may be essentially free of undifferentiated cells and thus suitable for use in cell-based therapies. | 10-11-2012 |
| 20120258452 | DNA-BASED MOLECULAR SWITCHES AND USES THEREOF - Disclosed are nucleic acid-based molecular switches that respond to changes in pH. The switches may be used in DNA nanodevices. The switches may also act as sensors for measuring the pH of a sample, including cells, regions thereof, and whole organisms. The switch includes an A-motif that forms at acidic pH. Also disclosed are compositions and methods for measuring the pH of cells or regions thereof, such as vesicles, the nucleus, mitochondrial matrix, or the Golgi lumen. | 10-11-2012 |
| 20120264115 | NORMALIZING CHROMOSOMES FOR THE DETERMINATION AND VERIFICATION OF COMMON AND RARE CHROMOSOMAL ANEUPLOIDIES - The present invention provides a method capable of detecting single or multiple fetal chromosomal aneuploidies in a maternal sample comprising fetal and maternal nucleic acids, and verifying that the correct determination has been made. The method is applicable to determining copy number variations (CNV) of any sequence of interest in samples comprising mixtures of genomic nucleic acids derived from two different genomes, and which are known or are suspected to differ in the amount of one or more sequence of interest. The method is applicable at least to the practice of noninvasive prenatal diagnostics, and to the diagnosis and monitoring of conditions associated with a difference in sequence representation in healthy versus diseased individuals. | 10-18-2012 |
| 20120264116 | METHODS AND APPARATUS FOR POINT-OF-CARE NUCLEIC ACID AMPLIFICATION AND DETECTION - Methods and apparatus are provided for point-of-care nucleic acid amplification and detection. One embodiment of the invention comprises a fully integrated, sample-to-answer molecular diagnostic instrument that optionally may be used in a multiplexed fashion to detect multiple target nucleic acid sequences of interest and that optionally may be configured for disposal after one-time use. Optionally, sample preparation is fully or partially achieved via heat treatment and/or using a filter paper, such as a chemically treated filter paper, e.g., an FTA card. The instrument preferable utilizes an isothermal nucleic acid amplification technique, such as loop-mediated isothermal amplification (LAMP), to reduce the instrumentation requirements associated with nucleic acid amplification. Detection of target amplification may be achieved, for example, via detection of a color shift or fluorescence in a dye added to the amplification reaction. | 10-18-2012 |
| 20120264118 | Quantitation of Cellular DNA and Cell Numbers Using Element Labeling - Methods and kits for the quantitation of cellular DNA and cell numbers are provided. Passive element uptake, element-labeled DNA intercalators, and element labeled affinity reagents are used to quantify DNA and cells. The DNA and the cells are analyzed by elemental analysis, including ICP-MS. The methods and kits provide a fast and accurate analysis of cellular DNA and cell numbers. | 10-18-2012 |
| 20120270209 | SYSTEMS, DEVICES, AND METHODS FOR SPECIFIC CAPTURE AND RELEASE OF BIOLOGICAL SAMPLE COMPONENTS - Living cells can be selectively and reversibly bound to functionalized dissolvable material (e.g., cross-linked hydrogel compositions) and subsequently released from the composition as viable cells. In some examples, the cells are released by reducing the degree of cross-linking within a functionalized hydrogel composition and/or dissolving the functionalized hydrogel composition bound to the cells. The functionalized hydrogel compositions can be adhered to silicon- and silicon-oxide containing surfaces, such as glass and aminated silicon. The living cells can be isolated from biological samples, such as blood, by selectively binding certain cells from the sample to the functionalized hydrogel, removing unbound cells and later releasing viable bound cells from the functionalized hydrogel. | 10-25-2012 |
| 20120270210 | METHOD AND REAGENT FOR GENE SEQUENCE ANALYSIS - Provided is a nucleic acid substrate which has nucleic acid substrate characteristics equivalent to those of dATP, has a low substrate specificity for luciferase, exerts no negative effect on enzymatic reactions such as a complementary-strand synthesis, and therefore is particularly suitable for the pyrosequencing method. As a nucleic acid substrate complementary to nucleotide T, a 7-substituted deoxyribonucleotide triphosphate whose 7-position of a purine group is modified by a substituent is used as a substitute for a nucleotide α-thiotriphosphate analog. | 10-25-2012 |
| 20120270211 | CHEMICALLY-ENHANCED PRIMER COMPOSITIONS, METHODS AND KITS - A chemically-enhanced primer is provided comprising a negatively charged moiety (NCM), an oligonucleotide sequence having a) non-nuclease resistant inter-nucleotide linkages or b) at least one nuclease resistance inter-nucleotide linkage. The chemically-enhanced primer can be used for sequencing and fragment analysis. Methods for synthesizing the chemically-enhanced primer as well as a method of preparing DNA for sequencing, a method of sequencing DNA, and kits containing the chemically-enhanced primer are also provided. The method of sequencing DNA can comprise contacting amplification reaction products with the composition wherein excess amplification primer is degraded by the nuclease and the chemically-enhanced primer is essentially non-degraded. | 10-25-2012 |
| 20120276527 | GENE RECOMBINATION SCREENING METHODS - Methods and compositions for detecting recombination events are disclosed. Methods and compositions for expressing a gene of interest are also disclosed. | 11-01-2012 |
| 20120282597 | BIOMARKER AND METHOD FOR EVALUATING RISK OF PROLIFERATION, INVASION, OR METASTASIS OF CANCER - The present invention relates to a biomarker associated with a cancer and a method using the biomarker to evaluate a risk of proliferation, invasion, or metastasis of a cancer. The method of the present invention comprises the following steps: (A) providing a tissue sample to evaluate for risk of proliferation, invasion, or metastasis of a cancer, wherein the tissue sample comprises a non-cancer region, and a suspected cancer region; (B) detecting expression levels of a biomarker and a predetermined standard in the non-cancer region and the suspected cancer region respectively, wherein the biomarker is T-cell lymphoma invasion and metastasis 2 (TIAM2); (C) comparing the expression levels of the biomarker and the predetermined standard in the non-cancer region to the expression levels of the biomarker and the predetermined standard in the suspected cancer region. | 11-08-2012 |
| 20120282598 | White Blood Cell Analysis System and Method - Systems and methods for analyzing blood samples, and more specifically for performing a white blood cell (WBC) differential analysis. The systems and methods screen WBCs by means of fluorescence staining and a fluorescence triggering strategy. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder WBC reagent(s), suitable for assays of samples containing fragile WBCs. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye, which corresponds in emission spectrum to an excitation source of a hematology instrument; (b) using a fluorescence trigger to screen the blood sample for WB Cs; and (c) using measurements of (1) axial light loss, (2) intermediate angle scatter, (3) 90° polarized side scatter, (4) 90° depolarized side scatter, and (5) fluorescence emission to perform a differentiation analysis. | 11-08-2012 |
| 20120282599 | Nucleated Red Blood Cell Analysis System and Method - Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs. | 11-08-2012 |
| 20120282600 | Basophil Analysis System and Method - Provided herein are systems and methods for analyzing blood samples, and more specifically for performing a basophil analysis. In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; and then (b) using measurements of light scatter and fluorescence emission to distinguish basophils from other WBC sub-populations. In one embodiment, the systems and methods include performing a basophil cluster analysis of the blood sample, based on the combination of light scatter and fluorescence measurements. | 11-08-2012 |
| 20120282601 | BLOOD ANALYZER, BLOOD ANALYSIS METHOD, AND COMPUTER PROGRAM PRODUCT - A blood analyzer, a blood analysis method, and a computer program product that can distinguishably detect abnormal lymphocytes, blasts, and atypical lymphocytes are provided. A blood analyzer prepares a first measurement sample from a first reagent containing a hemolyzing agent, a second reagent containing a fluorescence staining dye, and the blood specimen, and prepares a second measurement sample from a third reagent containing a hemolyzing agent, a fourth reagent containing a fluorescence staining dye, and the blood specimen. The blood analyzer measures each of the measurement samples, and distinguishably detects abnormal lymphocytes, blasts, and atypical lymphocytes in a blood specimen based on the measurement data. | 11-08-2012 |
| 20120282602 | MULTIPLE- ANALYTE ASSAY DEVICE AND SYSTEM - Provided herein is technology relating to testing biological samples and particularly, but not exclusively, to devices, systems, and kits for performing multiple, simultaneous real-time assays on a sample in a single-use disposable format. For example, the technology relates to an apparatus that finds use, for example, for point-of-care diagnostics, including use at accident sites, emergency rooms, in surgery, in intensive care units, as well as for non-medical applications. | 11-08-2012 |
| 20120282603 | AUTOMATED SYSTEM FOR ISOLATING, AMPLIFYING AND DETECTING A TARGET NUCLEIC ACID SEQUENCE - A system and method for preparing and testing of targeted nucleic acids is presented. The system integrates a pipetter, extractor, assay reader, and other components, including a selectively compliant articulated robot arm (SCARA). This synergistic integration of previously separate diagnostic tools creates a system and method whereby a minimum of human intervention is required. The resulting system provides a substantially more accurate and precise method of isolating, amplifying and detecting targeted nucleic acids for diagnosing diseases. | 11-08-2012 |
| 20120288853 | METHODS FOR OPERATING CHEMICALLY SENSITIVE SENSORS WITH SAMPLE AND HOLD CAPACITORS - Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis. | 11-15-2012 |
| 20120288854 | AUTOMATED HIGH-THROUGHPUT SEED SAMPLER AND METHODS OF SAMPLING, TESTING AND BULKING SEEDS - An automated system for sampling seeds includes a seed loading station for separating individual seeds from a plurality of seeds held in a seed hopper, an imaging station configured to receive the separated seeds from the seed loading station and collect image data of the received seeds, and a seed sampling subsystem configured to remove tissue samples from the seeds after the image data of the seeds is collected. And, an automated method for sampling seeds includes separating individual seeds from a plurality of seeds, imaging the separated seeds, and removing tissue samples from the imaged seeds. | 11-15-2012 |
| 20120288855 | Fluorescent Chemical Compounds Having High Selectivity for Double Stranded DNA, and Methods for Their Use - Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA. | 11-15-2012 |
| 20120295252 | Composition for Analyzing Nucleic Acid - A composition for analyzing nucleic acid that contains luciferase for which reactivity to dATP is equal to or less than 1/400 reactivity to ATP. A method for analyzing nucleic acid that comprises the use of the composition. A kit for analyzing nucleic acid comprising the composition. The method provides for an inexpensive, highly accurate and highly sensitive nucleic acid analysis that uses dATP instead of an expensive reagent having a low reactivity to DNA polymerase. | 11-22-2012 |
| 20120295253 | Dynamic Monitoring of Activation of Receptor Tyrosine Kinase (RTK) in Living Cells Using Real-Time Microelectronic Cell Sensing Technology - Methods for identifying a compound capable of interacting with a Receptor Tyrosine Kinase (RTK) including providing a device capable of measuring cell-substrate impedance, adding test cells expressing a RTK to the device, measuring first impedances, adding a compound to at least one compound well and adding a vehicle control at least one control well, measuring second impedances of the compound well and the control well, determining the change in the impedance for the compound well and the one control well, comparing the change in impedance between the compound well and the control well, and identifying the compound interacts with the RTK if the comparison demonstrates a significant difference between the change in impedance. | 11-22-2012 |
| 20120301873 | HUMAN LONG PENTRAXIN 3 EXPRESSION SYSTEM AND USES THEREOF - The present invention relates to an eukaryotic expression vector comprising a nucleotide sequence encoding for the human long pentraxin PTX3 protein under the control of an effective promoter and a nucleotide sequence encoding for a selectable marker, recombinant human cell able to provide expression of proteins encoded by the vector and method for the production of the human long pentraxin PTX3 protein. | 11-29-2012 |
| 20120308997 | SPATIALLY RESOLVED LIGAND-RECEPTOR BINDING ASSAYS - A method for analyzing the results of a ligand-receptor binding assay comprising the steps of:
| 12-06-2012 |
| 20120315628 | METHOD FOR IDENTIFYING ONCOGENE, METHOD FOR ESTABLISHING ONCOGENE-EXPRESSING CELL, AND METHOD OF SCREENING ONCOGENE TARGETING DRUG - The present invention provides a methodology that can develop a more excellent anti-cancer drug than conventional ones for various cancers. Specifically, the present invention provides a method for establishing an artificial cell, comprising treating a cancer cell with an expression vector for a foreign oncogene and then culturing the cancer cell treated with the expression vector under a condition which inhibits an expression or function of an oncogene that is inherent to the cancer cell, and an established artificial cell; a method of screening an anti-cancer drug, comprising evaluating whether or not a test substance inhibits a proliferation of the artificial cell; as well as a method for identifying an oncogene, comprising treating a cancer cell with an expression vector for a test gene and then culturing the cancer cell treated with the expression vector under a condition which inhibits an expression or function of an oncogene that is inherent to the cancer cell. | 12-13-2012 |
| 20120322054 | Methods and Apparatus for Measuring Analytes Using Large Scale FET Arrays - Methods and apparatus relating to very large scale FET arrays for analyte measurements. ChemFET (e.g., ISFET) arrays may be fabricated using conventional CMOS processing techniques based on improved FET pixel and array designs that increase measurement sensitivity and accuracy, and at the same time facilitate significantly small pixel sizes and dense arrays. Improved array control techniques provide for rapid data acquisition from large and dense arrays. Such arrays may be employed to detect a presence and/or concentration changes of various analyte types in a wide variety of chemical and/or biological processes. In one example, chemFET arrays facilitate DNA sequencing techniques based on monitoring changes in hydrogen ion concentration (pH), changes in other analyte concentration, and/or binding events associated with chemical processes relating to DNA synthesis. | 12-20-2012 |
| 20120322055 | Molecular Dispensers - A method for dispensing charged particles includes applying a bias voltage to promote motion of charged molecules through a nanopore, detecting passage of at least one charged molecule through the nanopore, and manipulating an electrostatic potential barrier inside the nanopore, so as to prevent movement of additional charged molecules through the nanopore. | 12-20-2012 |
| 20120322056 | RNA-EXIT-CHANNEL: TARGET AND METHOD FOR INHIBITION OF BACTERIAL RNA POLYMERASE - The invention provides a target and methods for specific binding and inhibition of RNAP from bacterial species. The invention is directed to a method for identifying agents that bind to a bacterial RNAP homologous RNA-exit-channel amino-acid sequence, comprising preparing a reaction solution comprising the agent to be tested and an entity comprising a bacterial RNAP homologous RNA-exit-channel amino-acid sequence, and detecting presence or amount of binding. The invention has applications in control of bacterial gene expression, control of bacterial growth, antibacterial chemistry, and antibacterial therapy. | 12-20-2012 |
| 20120322057 | POLYMERASE COMPOSITIONS AND METHODS - Disclosed herein are modified polymerase compositions exhibiting altered polymerase activity, which can be useful in a variety of biological applications. Also disclosed herein are methods of making and using such compositions. In some embodiments, the compositions exhibit altered properties that can enhance their utility in a variety of biological applications. Such altered properties, can include, for example, altered nucleotide binding affinities, altered nucleotide incorporation kinetics, altered photostability and/or altered nanoparticle tolerance, as well as a range of other properties as disclosed herein. | 12-20-2012 |
| 20120329042 | METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION - Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule. | 12-27-2012 |
| 20120329043 | ACTIVE CHEMICALLY-SENSITIVE SENSORS WITH SOURCE FOLLOWER AMPLIFIER - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 12-27-2012 |
| 20120329044 | ACTIVE CHEMICALLY-SENSITIVE SENSORS WITH CORRELATED DOUBLE SAMPLING - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 12-27-2012 |
| 20130004945 | SELECTIVE INHIBITORS OF TRANSLESION DNA REPLICATION - An agent for inhibiting translesion DNA replication comprises a non-natural adenine ribose analog represented by those as set forth in FIG. | 01-03-2013 |
| 20130004946 | Compositions and Methods for Genetic Manipulation and Monitoring of Cell Lines - The disclosure relates generally to stem cell biology and more specifically to genetic manipulation of stem cells. Methods and compositions using recombinational cloning techniques are disclosed which allow the construction and insertion of complex genetic constructs into embryonic and adult stem cells and progenitor cells. The methods disclosed will allow the harvesting of adult stem cells pre-engineered with integration sites to facilitate early passage genetic modification. | 01-03-2013 |
| 20130004947 | LUCIFERASE-LINKED ANALYSIS OF DNA-METHYLTRANSFERASE, PROTEIN METHYLTRANSFERASE AND S-ADENOSYLHOMOCYSTEINE AND USES THEREOF - The present invention relates to methods for detecting the activity of DNA (cytosine 5)-methyltransferase, protein methyltransferase or any enzyme that forms S-adenosyl-1-homocysteine (AdoHcy) as a product, and for screening for inhibitors of DNA (cytosine 5)-methyltransferase, protein methyltransferase and any enzyme that forms S-adenosyl-1-homocysteine (AdoHcy) as a product using luciferase-linked assays that convert the S-adenosyl-1-homocysteine (AdoHcy) product to a quantifiable luminescent signal. | 01-03-2013 |
| 20130004948 | ACTIVE CHEMICALLY-SENSITIVE SENSORS WITH RESET SWITCH - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 01-03-2013 |
| 20130004949 | METHODS AND APPARATUS FOR HIGH SPEED OPERATION OF A CHEMICALLY-SENSITIVE SENSOR ARRAY - Methods and apparatus relating to FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 01-03-2013 |
| 20130017537 | METHOD OF IN-VITRO IMMUNOASSAY - The present invention relates to a novel method of in-vitro assay of the molecules of the extracellular matrix synthesized by cells in culture and uses thereof in the form of a kit for in-vitro measurement and/or for a method of screening of cosmetic and/or pharmaceutical ingredients. | 01-17-2013 |
| 20130022965 | TEST SYSTEM FOR VISUAL ANALYSIS - The present invention relates to a test system for visual analysis and to the use thereof in the point-of-care testing field. | 01-24-2013 |
| 20130022966 | Methods of Treating Cancer Using Notch Pathway Inhibitors - The present invention is based on the discovery that the Notch signaling pathway is associated with cancer. Accordingly, the invention provides methods and compositions for treating cancer. Also provided are methods of modulating the expression and/or activity of proteins in the Notch signaling pathway for use in diagnoses and treatment of cancer in a subject. | 01-24-2013 |
| 20130022967 | NUCLEIC ACID MOLECULE HAVING AFFINITY TO RODENT-DERIVED IgG ANTIBODY, BINDER, DETECTION REAGENT, AND DETECTION KIT - The invention provides a nucleic acid molecule having a binding affinity to a rodent-derived IgG antibody, which can be prepared easier than an antibody and has a binding affinity equivalent or superior to that of an antibody, a binder using the nucleic acid molecule, a detection reagent, and a detection kit. The nucleic acid molecule of the invention has a binding affinity to a rodent-derived IgG antibody and has a dissociation constant of 1 μM or less. The binder for a rodent-derived IgG antibody of the present invention includes the nucleic acid molecule of the present invention. The detection reagent for detecting a rodent-derived IgG antibody of the invention includes the binder for a rodent-derived IgG antibody of the invention. The detection kit for detecting a rodent-derived IgG antibody of the invention includes the detection reagent for detecting a rodent-derived IgG antibody of the invention. | 01-24-2013 |
| 20130022968 | MODULAR NUCLEOTIDE COMPOSITIONS AND USES THEREFOR - Nucleic acid compositions, methods of making and using such compositions that comprise modular functional groups that can be configured to provide desired functionality to different nucleotide types, through a swappable and preferably non-covalent linkage component. Such compositions are useful in a variety of applications including nucleic acid analyses. | 01-24-2013 |
| 20130022969 | SINGLE-PAD STRIP FOR AN IMPROVED LATERAL FLOW ASSAY AND A TEST DEVICE USING THE SAME - The present invention relates to a strip for an improved lateral flow assay of a biological sample on a single plane and a lateral flow chromatography assay using a test device containing the same. The strip of the present invention consists of a single-pad, which can improve lateral flow assay by providing an easy and simple procedure and clear visual reading. The strip of the present invention is consisted of sample application (sample) zone and reactant-resultant zone where the reaction mixture is deposited (reactant) are all on a same plane. In addition, the present invention provides a chromatographic method wherein hemoglobin is separated from analyte by a differential chromatography on the solid phase. Any interference of detection of the result by hemoglobin is removed by the present invention. The present invention provides advantages including an easy and simple procedure with a quick and clear response. | 01-24-2013 |
| 20130029323 | SYSTEMS, DEVICES, AND METHODS FOR AUTOMATED CHARACTERIZATION OF A NUCLEIC ACID MOLECULE - The invention generally relates to systems, cartridges, and methods for automated characterization of a nucleic acid molecule, in particular, optical mapping of DNA from an organism. In certain embodiments, the invention provides a cartridge for characterizing a nucleic acid molecule, the cartridge including a reaction chamber having a derivatized bottom surface, at least one reagent reservoir, and a pump, in which the reaction chamber, the reagent reservoir, and the pump are fluidically connected to each other. | 01-31-2013 |
| 20130029324 | LIVE BIOLOAD DETECTION USING MICROPARTICLES - The present invention provides methods to concentrate cells onto microparticles, to concentrate the microparticles, and to detect the cells. The present invention also includes unitary sample preparation and detection devices to be used in accordance with the methods. | 01-31-2013 |
| 20130029325 | FLUORESCENT PROBE FOR PLASMA CELL IDENTIFICATION AND ISOLATION, AND PLASMA CELL IDENTIFICATION OR ISOLATION METHOD USING THE PROBE - A method which can isolate plasma cells and plasmablasts efficiently and with high purity, from mammals and birds, without using a cell surface marker is provided. | 01-31-2013 |
| 20130029326 | Selective 5' Ligation Tagging of RNA - The present invention provides novel compositions, kits and methods employing RNA 5′ polyphosphatases, RNA 5′ monophosphatases, capping enzymes, decapping enzymes, nucleic acid pyrophosphatases and RNA ligases, as well as other enzymes, for selective 5′ ligation tagging of desired classes of RNA molecules that differ with respect to particular chemical moieties on their 5′ ends. The 5′ tagged RNA molecules can be used for synthesis of tagged first-stand cDNA, double-stranded cDNA, and sense or antisense RNA for a variety of uses. | 01-31-2013 |
| 20130034846 | MOLECULAR BIOSENSORS CAPABLE OF SIGNAL AMPLIFICATION - The present invention provides molecular biosensors capable of signal amplification, and methods of using the molecular biosensors to detect the presence of a target molecule. | 02-07-2013 |
| 20130034847 | OLIGONUCLEOTIDE BASED ANALYTE DETECTION METHOD - The invention relates to a method of detecting the presence of an analyte within a sample, wherein said method comprises an oligonucleotide based approach. The invention also relates to methods of diagnosing diseases, in particular, but not exclusively infectious diseases such as septicaemia. | 02-07-2013 |
| 20130040291 | Detection and Quantification of Cellular Stress Response Resolution Pathway Expression and Functionality and the Effects of Agents or Conditions Thereupon - The assessment methods disclosed herein involve exposing cells to two stresses. The first stress (analogous to existing direct screening methods) involves exposing metazoan cells to an external stimulus of interest, such as exposing human cells to ionizing radiation, a chemical, or a set of physical conditions. The cells exposed to the first stress are thereafter exposed to a second stress which, importantly, is calibrated such that non-stressed cells of the same type will exhibit a predictable response to the second stress. By comparing the response to the second stress of i) cells that were exposed to the first stress and ii) cells that were not exposed to the first stress, an observer can assess whether exposure to the first stress caused or induced any change to the cells that affected the cells' ability to respond to the second stress. | 02-14-2013 |
| 20130045475 | 2-Nitrobenzyl-Modified Ribonucleotides - This disclosure provides novel reversibly terminated ribonucleotides which can be used as a reagent for DNA sequencing reactions. Methods of sequencing nucleic acids using the disclosed nucleotides are also provided. | 02-21-2013 |
| 20130052638 | Methods for detecting DNA-binding proteins - There is provided a method for detecting binding of a DNA-binding protein to a target recognition sequence. The method comprises mixing in a reaction buffer a first set of metal nanoparticles, a second set of metal nanoparticles and a DNA-binding protein to form a mixture, and detecting the aggregation state of the mixture of metal nanoparticles. Each set of metal nanoparticles has a conjugated double-stranded DNA molecule having a single-stranded overhang at one end. The single-stranded overhangs of each set of DNA-conjugated metal nanoparticles are complementary to each other such that annealing of the complementary overhangs results in formation of the target recognition sequence that specifically binds the DNA-binding protein. The reaction buffer comprises an ionic species in a concentration sufficient to result in aggregation of the metal nanoparticles upon annealing of the first and second single-stranded overhang. | 02-28-2013 |
| 20130052639 | METHOD OF ANALYZING XPG ENDONUCLEASE ACTIVITY - A method of quantitatively analyzing an XPG endonuclease activity is provided. The XPG endonuclease activity can be simply and cheaply analyzed without undergoing overexpression or purification of a recombinant protein. | 02-28-2013 |
| 20130052640 | CLOSTRIDIUM DIFFICILE DEHYDROGENASE AND TOXIN AS A BIOMARKER FOR MONITORING INFECTION IN PATIENTS WITH CLOSTRIDIUM DIFFICILE DISEASE AND DIFFERENTIATING CARRIER STATE FROM ACTIVE DISEASE - disease involves a range of clinical presentations ranging from carrier status with other causes of symptoms to mild and self-limiting diarrhea to life-threatening pseudomembranous colitis and megacolon. Cases of | 02-28-2013 |
| 20130059295 | Host Cells and Methods for Producing Fatty Acid - The present invention provides for a genetically modified host cell capable of producing fatty acid comprising an increased expression of FadR, or a functional variant thereof. The host cell under environmental conditions wherein fatty acid is produced expresses an increased amount of FadR when compared to an unmodified host cell. The present invention also provides for a method of producing a fatty acid or FAAE in the host cell. The present invention provides for a genetically modified host cell comprising a fatty acid biosensor and one or more fatty acid-responsive promoter operably linked to one or more genes of interest that is heterologous to the fatty acid-responsive promoter. | 03-07-2013 |
| 20130059296 | Compositions and Methods For High Fidelity Assembly of Nucleic Acids - Aspects of the invention relate to methods, compositions and algorithms for designing and producing a target nucleic acid. The method can include: (1) providing a plurality of blunt-end double-stranded nucleic acid fragments having a restriction enzyme recognition sequence at both ends thereof; (2) producing via enzymatic digestion a plurality of cohesive-end double-stranded nucleic acid fragments each having two different and non-complementary overhangs; (3) ligating the plurality of cohesive-end double-stranded nucleic acid fragments with a ligase; and (4) forming a linear arrangement of the plurality of cohesive-end double-stranded nucleic acid fragments, wherein the unique arrangement comprises the target nucleic acid. In certain embodiments, the plurality of blunt-end double-stranded nucleic acid fragments can be provided by: releasing a plurality of oligonucleotides synthesized on a solid support; and synthesizing complementary strands of the plurality of oligonucleotides using a polymerase based reaction. | 03-07-2013 |
| 20130059297 | METHOD FOR ISOLATING RENAL STEM/PROGENITOR CELLS, RENAL STEM/PROGENITOR CELLS AND THERAPEUTIC AGENT FOR RENAL DISEASE - It is intended to provide a method for noninvasively isolating human renal stem/progenitor cells, an isolated renal stem/progenitor cells, a therapeutic agent for renal disease, mouse mesenchymal cells which can be used for isolating human renal stem/progenitor cells and a culture supernatant of the same. Renal stem/progenitor cells are isolated by primarily culturing cells contained in the urine of a patient having renal disease in a medium containing mouse mesenchymal cells identified by the deposition number of FERM ABP-10865 or a culture supernatant of the same, staining the obtained primarily cultured cells with Hoechst 33342 and separating a weak-positive or negative fraction. | 03-07-2013 |
| 20130059298 | Composition and Method for Treatment of Tumors - The present invention relates to a composition which is useful in the treatment of a tumor, a method for making such a composition, and a method for using such a composition. The invention relates also to a method for assaying for inhibitors of the activity of Core 1 protein and/or other proteins of the respiratory complex III of mitochondria. | 03-07-2013 |
| 20130065225 | Reversible Di-Nucleotide Terminator Sequencing - The present teachings provide methods, compositions, and kits for synthesizing and sequencing nucleic acids. In some embodiments, reversible di-nucleotide compounds are employed along with cleaving reactions that remove a label and a blocking moiety. Improved sequencing efficiency is achieved by the rapid polymerase-mediated incorporation of reversible di-nucleotide compounds. In some embodiments, the di-nucleotides do not contain conventional nucleotide triphosphates, but rather employ amino acid phosphoramidate nucleotides (AAPNs). | 03-14-2013 |
| 20130065226 | METHOD OF DETERMINING DOSE AND ADMINISTRATION OF STATIN - The object of the present invention is to provide a method of determining the dose and/or administration of statins to a patient suffering from a cardiovascular disease. | 03-14-2013 |
| 20130065227 | METHODS OF INCREASING MACROPINOCYTOSIS IN CANCER CELLS - This disclosure describes methods of stimulating macropinocytosis in cancer cells. | 03-14-2013 |
| 20130071835 | Qualitative and Quantitative Analytical Method for Analyzing the Activity Type of an Enzyme that is Activated by Proteolysis - The present invention relates to a qualitative and quantitative analytical method for analyzing the activity type of an enzyme that is activated by proteolysis. The method of the present invention is advantageous in that the activity type of an enzyme present in a specimen is analyzed using an enzyme activity inhibitor and a binder to enable the analysis to be performed in a quicker and more accurate manner as compared to enzyme-activity analysis methods using a simple binder (for example, an antibody). The method of the present invention can enable the analysis of the activity type of an enzyme present in a specimen in a simple manner using less equipment than enzyme-activity analysis methods using a simple binder. The method of the present invention involves simultaneously using the inhibitor and the binder in analyzing the activity type of a target enzyme or an enzyme to enable quick and specific analysis, and high-throughput drug screening can be performed at a high speed due to the above-described characteristics of the method of the present invention. In addition, the method of the present invention can be applied to point-of-care testing (POCT) for the clinical monitoring of the effects and dosage of a drug in a drug administration target (for example, an animal or human). | 03-21-2013 |
| 20130078621 | DETECTION COMPOSITION - The invention provides methods, kits, devices and compositions for detecting one or more target analytes. In some embodiments, the invention provides binding elements and labeling elements capable of being joined through a plurality of joining elements. | 03-28-2013 |
| 20130078622 | ELECTRONIC DEVICE FOR MONITORING SINGLE MOLECULE DYNAMICS - A single molecule sensing device includes a first electrode, a second electrode and a single-walled carbon nanotube (SWNT) connected to the first and second electrodes. At least one linker molecule having first and second functional groups is functionalized with a sidewall of the SWNT, the at least one linker molecule having the first functional group non-covalently functionalized with a sidewall of the single-walled carbon nanotube. A single sensitizing molecule having at least one functional group is functionalized with the second functional group of the at least one linker molecule. | 03-28-2013 |
| 20130078623 | BIOLOGICAL ANALYSIS ARRANGEMENT AND APPROACH THEREFOR - Characteristics of a chemical or biological sample are detected using an approach involving light detection. According to an example embodiment of the present invention, an assaying arrangement including a light detector is adapted to detect light from a sample, such as a biological material. A signal corresponding to the detected light is used to characterize the sample, for example, by detecting a light-related property thereof. In one implementation, the assaying arrangement includes integrated circuitry having a light detector and a programmable processor, with the light detector generating a signal corresponding to the light and sending the signal to the processor. The processor provides an output corresponding to the signal and indicative of a characteristic of the sample. | 03-28-2013 |
| 20130084561 | BIODETECTION BY NUCLEIC ACID-TEMPLATED CHEMISTRY - The invention provides compositions and methods for the detection of biological targets, (e.g. nucleic acids and proteins) by nucleic acid templated chemistry, for example, by generating fluorescent, chemiluminescent and/or chromophoric signals. | 04-04-2013 |
| 20130084562 | TETHERED ENZYME MEDIATED NUCLEIC ACID DETECTION - The present invention relates to methods and compositions for detecting a target nucleotide sequence in a sample. In particular, the methods and compositions of the present invention employ a reporter enzyme tethered to a solid support via a tether nucleotide sequence. A disclosed method for detecting a target nucleotide sequence comprises providing a linker nucleotide sequence comprising a probe sequence that can hybridize to the target sequence and a trigger which can hybridize to the tether nucleotide sequence when the probe sequence binds to the target. The linker nucleotide can have a stem-loop structure. When the trigger hybridizes to the tether nucleotide sequence, the tether is able to be digested by a nuclease, releasing the reporter enzyme from the solid support. The activity of the released reporter enzyme on a spatially or temporally separated substrate generates a detectable signal. In some embodiments, the reporter enzyme releases a feedback trigger that can hybridize to the tether nucleotide sequence and release further reporter enzymes, amplifying the signal via a feedback loop. | 04-04-2013 |
| 20130084563 | CO-CULTURING MAMMALIAN EMBRYONIC STEM CELLS WITH HUMAN FORESKIN FIBROBLASTS - A cell culture comprising human foreskin cells, the human foreskin cells being capable of maintaining stem cells in an undifferentiated state when co-cultured therewith. | 04-04-2013 |
| 20130089856 | METHOD FOR IN VITRO DETECTION OF ATAXIA TELANGIECTASIA HEALTHY CARRIERS AND RELATED KIT - The present invention concerns a method for in vitro detection of ataxia telangiectasia healthy carriers by determination of cell percentage wherein p53 is delocalized from centrosome during the mitosis. | 04-11-2013 |
| 20130089857 | METHOD FOR DETECTING THE PRESENCE OF SPECIFIC MICRO-ORGANISMS AND DEVICE FOR THE SAME - A method of testing for the presence of a preselected target nucleic acid, protein or antigen in a biological sample by exposing nucleic acids, proteins or antigens to a probe having a catalytic element and binding element. The catalytic element catalyses at least one reaction that results in a physical change such that identifiable elements provide an indication of the presence of the target. | 04-11-2013 |
| 20130089858 | MOLECULAR DIAGNOSTIC ASSAY DEVICE AND METHOD OF USE - Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing. | 04-11-2013 |
| 20130095471 | 3'-OH UNBLOCKED NUCLEOTIDES AND NUCLEOSIDES BASE MDIFIED WITH NON-CLEAVABLE, TERMINATING GROUPS AND METHODS FOR THEIR USE IN DNA SEQUENCING - Provided are novel nucleotides, nucleoside, and their derivatives described herein, that can be used in DNA sequencing technology and other types of DNA analysis. In one embodiment, the nucleotide or nucleoside with an unprotected 3′-OH group is derivatized at the nucleobase to include a fluorescent dye attached via a linker to a non-cleavable terminating group. The non-cleavable-fluorescent group is designed to terminate DNA synthesis so that DNA oligomers can be sequenced efficiently in a parallel format. These reagents and methods will lead to more accurate identification of polymorphisms and other valuable genetic information. | 04-18-2013 |
| 20130095472 | Cytokines and Genes Differentially Affected by TNF Blockers - The present invention is directed to cytokines and genes that are differentially affected by TNF blockers and the use of these genes and cytokines to help asses the TB risks of new immunosuppressive therapies, to help evaluate the effects of new TB vaccines, and to help assess TB susceptibility in persons exposed to | 04-18-2013 |
| 20130095473 | METHOD FOR DETERMINATION OF TARGET CELLS OR TISSUE FOR EXTRACTION OF BIOMOLECULES FROM FIXED BIOLOGICAL SAMPLES - The present invention relates to a method for determination of target cells or tissue for isolating or extracting biomolecules from fixed biological samples, the preparation of a sample in a method for extracting, isolating and/or purifying biomolecules from a fixed biological sample as well as to a kit for visualizing target cells or tissue in a fixed biological sample for extracting, isolating and/or purifying biomolecules from said target cells or tissue. | 04-18-2013 |
| 20130101994 | Method of Analysing a Cell or Other Biological Material Containing a Nucleic Acid - According to the invention there is provided a compound of Formula (I) in which: A is a C | 04-25-2013 |
| 20130101995 | DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT - The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid. | 04-25-2013 |
| 20130101996 | DETECTION METHOD AND APPARATUS OF ACTIVATED NEUTROPHILS - To provide a detection method of activated neutrophils enabling the classification of normal leukocytes and the detection of activated neutrophils, a measurement sample in which erythrocytes in a biotic sample are hemolyzed and nucleic acid of leukocytes is stained by a nucleic acid staining fluorochrome is prepared, the sample is irradiated with light, and leukocytes in the biotic sample are classified into at least a cluster containing neutrophils and a cluster containing eosinophils based on scattered light intensity and fluorescence intensity obtained by measuring the scattered light intensity and fluorescence intensity generated from particles in the sample to detect particles present between the cluster containing neutrophils and the cluster containing eosinophils as activated neutrophils. | 04-25-2013 |
| 20130109011 | METHODS OF AND DEVICES FOR CAPTURING CIRCULATING TUMOR CELLS | 05-02-2013 |
| 20130109012 | DEVICE TO EXPOSE CELLS TO FLUID SHEAR FORCES AND ASSOCIATED SYSTEMS AND METHODS | 05-02-2013 |
| 20130122489 | 3'-OH UNBLOCKED, FAST PHOTOCLEAVABLE TERMINATING NUCLEOTIDES AND METHODS FOR NUCLEIC ACID SEQUENCING - The present invention relates generally to 3′-OH unblocked nucleotides and nucleosides labeled and unlabeled with 5-methoxy-substituted nitrobenzyl-based photocleavable terminating groups for use in methods and systems related to DNA and RNA sequencing and analysis. These compounds may be used as reversible terminators as they exhibit fast nucleotide incorporation kinetics, single-base termination, high nucleotide selectivity, and rapid terminating group cleavage that results in a naturally occurring nucleotide. | 05-16-2013 |
| 20130122490 | PHOSPHOLINK NUCLEOTIDES FOR SEQUENCING APPLICATIONS - The present invention provides labeled phospholink nucleotides that can be used in place of naturally occurring nucleotide triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays, and others. | 05-16-2013 |
| 20130122491 | SYSTEMS AND METHODS FOR DETECTION OF CELLULAR STRESS - There are provided methods for detection and measurement of stress in a cell, the method including introducing a labeled tRNA into the cell and detecting a change in subcellular localization of the labeled tRNA in the cell, based on the signal emitted from the labeled tRNA. There are further provided methods and systems for the generation of a stress index of a living cell. There are further provided methods and systems for detection of stress in a living cell, comprising detection of changes in subcellular localization of labeled tRNA in a cell, wherein the detection is performed in real time. | 05-16-2013 |
| 20130130236 | SEPARATION METHOD OF LABELED CELLS AND USES THEREOF - A method for separating labeled cells and a use (of the labeled cells) thereof are provided. More specifically, a method for labeling cells using fluorescent magnetic nanodiamonds, and a method for separating the labeled cells using the labeling method by the fluorescent or magnetic properties of the nanodiamonds are provided. | 05-23-2013 |
| 20130130237 | APPARATUS AND METHOD FOR ANALYZING STATE OF DNA - The present invention provides an analysis apparatus including: an irradiation section for irradiating the chromatin structure with terahertz waves; a detection section for acquiring a set of terahertz wave spectral information from the chromatin structure; a memory section for memorizing the sets of terahertz wave spectral information corresponding to the states of the chromatin structure; and a data processing section for analyzing the state of the chromatin structure by comparing the set of spectral information acquired in the detection section and the sets of spectral information memorized in the memory section. | 05-23-2013 |
| 20130130238 | Fluorimetric Process for Evaluating the Influence of A Condition on A Biological Sample and Applications Thereof - The present invention relates to a process for determining the influence of a condition on a biological sample comprising a step consisting in establishing the kinetic profile of the fluorescence emitted, during the excitation at a suitable excitation wavelength, of a fluorescent compound bound to said biological sample, said sample having been, prior to said excitation, subjected to said condition and said process not necessitating the utilization of a fluorescence donor component and of a different fluorescence acceptor component. The present invention also relates to the various applications of such a process. | 05-23-2013 |
| 20130137091 | Methods And Compositions For Incorporating Nucleotides - The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses. | 05-30-2013 |
| 20130137092 | DEVICE FOR DETECTING AND SEPARATING TARGET MOLECULES AND METHOD FOR DETECTING AND SEPARATING TARGET MOLECULES BY USING THE SAME - A device, system, and method for detecting or separating target molecules allowing efficient detection even when only a small amount of target molecules or target cells are included in a sample involving the use of a target molecule linkage portion, a signal production portion, and first and second separation portions. | 05-30-2013 |
| 20130137093 | SYSTEMS AND METHODS FOR MANAGING INVENTORIES OF REAGENTS - A system for managing the inventory of reagents for a laboratory automation system. The system for managing the inventory of reagents comprises a controller, software for the controller, and a refrigerator capable of refrigerating reagents, detecting the presence or absence of reagents in the refrigerator, and detecting the location of reagents in the refrigerator. The system for managing the inventory of reagents is connected to a laboratory automation system. The laboratory automation system comprises at least one clinical analyzer. A typical system for managing inventories of reagents includes an operator interface for the loading of boxes of reagents and other supplies, radio frequency identification system for identification of inventory and tracking, robotic mechanisms for loading containers onto the track system and removing containers from the track system, de-capping equipment, refrigeration equipment, and information technology connections to laboratory analyzers and vendors. | 05-30-2013 |
| 20130143206 | Integrated Analytical System and Method - An analytical assembly within a unified device structure for integration into an analytical system. The analytical assembly is scalable and includes a plurality of analytical devices, each of which includes a reaction cell, an optical sensor, and at least one optical element positioned in optical communication with both the reaction cell and the sensor and which delivers optical signals from the cell to the sensor. Additional elements are optionally integrated into the analytical assembly. Methods for forming and operating the analytical system are also disclosed. | 06-06-2013 |
| 20130149698 | STABLE COMPOSITIONS FOR NUCLEIC ACID AMPLIFICATION AND SEQUENCING - The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size. | 06-13-2013 |
| 20130149699 | Translation Kinetic Mapping, Modification and Harmonization - The profile of translation elongation rate along an mRNA is modulated in a directed manner by locally altering codon usage, in particular utilizing differences in ribosomal dwell times among pairs of synonymous codons translated by a single tRNA through wobble base pairing. Unlike codon optimization based on organism-specific codon frequencies or tRNA pools, the methods of the invention need not change the tRNA that translates the codon, rather modulating the interaction between a given tRNA and the mRNA coding sequence. | 06-13-2013 |
| 20130149700 | MEASUREMENT OF AUTOANTIBODIES AT LOW CONDUCTIVITY WITH INCREASED SENSITIVITY - Methods for detecting or capturing low-avidity autoantibodies in a biological sample are provided. Target antigen used to assay for the low-avidity autoantibodies of interest is immobilized on a solid phase. The biological sample is contacted under low conductivity condition with the target antigen for which the autoantibodies has specific binding affinity. Binding of the target antigen to the autoantibodies of interest in the biological sample is then detected to ascertain the presence or concentration of the autoantibodies of interest. | 06-13-2013 |
| 20130149701 | METHODS OF IDENTIFYING THERAPEUTIC AGENTS FOR TREATING PERSISTER AND BACTERIAL INFECTION - The present invention relates to methods, compositions, assays and kits for identifying an antibacterial agent that decreases persister formation or survival, eliminates or reduces bacterial infection or disease and/or increases killing of a bacterial cell. | 06-13-2013 |
| 20130157260 | IDENTIFICATION OF COMPOUNDS THAT REGULATE APOPTOSIS - An assay for identifying compounds that affect conversion of Bcl-2 family protein from an antiapoptotic to a proapoptotic form or inhibit activity of Bcl-2 family protein is described. | 06-20-2013 |
| 20130157261 | Compositions and Methods for Quantitative Histology, Calibration of Images in Fluorescence Microscopy, and ddTUNEL Analyses - Disclosed are compositions and methods for quantitation and calibration of images in fluorescence microscopy. Also provided are tissue phantoms that contain known amount(s) of fluorophore standard(s), as well as components and diagnostic kits containing the same for use in various histological analyses. In certain embodiments, three distinct nucleic-acid based assays provide improvements over conventional TUNEL methods to facilitate precise quantitation of a variety of nucleic acids obtained from a biological sample. | 06-20-2013 |
| 20130157262 | Method for Selecting Antibody-Producing Cell Line, and Kit Thereof - Provided is a method for selecting antibody-producing cell lines using a split fluorescent protein, and a kit for selecting antibody-producing cell lines. By using the split fluorescent protein to select the antibody-producing cell lines, the antibody-producing cell lines can be easily detected by observing whether a single fluorescent color derived from reassembly of the split fluorescent proteins is expressed, which leads in a drastic reduction in selection time and cost required to select highly productive antibody-producing cell line. | 06-20-2013 |
| 20130157263 | METHODS AND COMPOSITIONS FOR SEAMLESS CLONING OF NUCLEIC ACID MOLECULES - The present invention is in the fields of biotechnology and molecular biology. More particularly, the present invention relates to cloning or subcloning one or more nucleic acid molecules comprising one or more type IIs restriction enzyme recognition sites. The present invention also embodies cloning such nucleic acid molecules using recombinational cloning methods such as those employing recombination sites and recombination proteins. The present invention also relates to nucleic acid molecules (including RNA and iRNA), as well as proteins, expressed from host cells produced using the methods of the present invention. | 06-20-2013 |
| 20130157264 | NUCLEIC ACID ANALYSIS DEVICE, NUCLEIC ACID ANALYSIS APPARATUS, AND NUCLEIC ACID ANALYSIS METHOD - In a nucleic acid analysis device which detects a fluorescent dye on a nucleic acid sample immobilized on a surface of a substrate by exciting the fluorescent dye with an evanescent wave, the detection of a fluorescence signal with a high SN ratio is realized even for a long nucleic acid sample. | 06-20-2013 |
| 20130164738 | Genetic Sample Collection Systems - Biological sample collection kits are devised with physical features to enable a high performance collection system which delivers preprocessed biological matter via conventional shipping means to a testing laboratories. In particular, untrained and unskilled users deposit biological matter such as saliva or blood into a receiving vessel. By sealing the container, the user causes release of a premixed solution containing preservatives and optionally lysis reagents. In addition, a purification agent is arranged to bind to target molecules and facilitate their removal from solution. These time consuming processes occur while the sample is in transit to the testing facility such that when it arrives, it is in a preconditioned state immediately ready for execution of washing steps. Thus, the high performance containers taught herein are useful for collection biological samples and performing initial process steps on received matter—steps which are largely effected during the shipping stage of the transfer process. | 06-27-2013 |
| 20130164739 | SMALL MOLECULE-DEPENDENT SPLIT APTAMER LIGATION - Methods, assays, and products for the detection of small molecules are provided. In one aspect, for example, a method of detecting a small molecule in a sample can include reacting together a first half of a DNA split aptamer having a first reactive group coupled thereto, a second half of a DNA split aptamer having a second reactive group coupled thereto, where the DNA split aptamer is selective for the small molecule, and a sample containing the small molecule. The first half and the second half bind to the small molecule and the first reactive group and the second reactive group react to form an aptamer ligation product of the first half and the second half. The method can also include assaying for the aptamer ligation product in order to detect the small molecule presence in the sample. | 06-27-2013 |
| 20130164740 | Cellular Analysis of Body Fluids - Herein is provided a simple, reliable and accurate method for cellular analysis on hematology analyzers. In various aspects, the methods provide separation and/or differentiation between red blood cells (RBCs) and white blood cells (WBCs) by utilizing a fluorescent dye to selectively stain WBCs such that they emit stronger fluorescence signals. The method provides optimal detection limits on WBCs and RBCs, thereby allowing analysis of samples with sparse cellular concentrations. As few as one reagent may be used to prepare a single dilution for body fluid analysis, in order to simplify the body fluid analysis. Minimal damage to WBCs is attained using the lysis-free approach described in aspects of the disclosure. | 06-27-2013 |
| 20130171626 | METHODS OF EVALUATING GENE EXPRESSION LEVELS - Described herein are methods of evaluating the expression levels of DNA parts encoding proteins in test circuits. In particular, the methods disclosed herein are useful to evaluate the expression of an output protein regulated by a regulatory protein-genetic element pair. | 07-04-2013 |
| 20130171627 | Method for Producing Dendritic Cells - Disclosed are embryonic stem cell-derived dendritic cells, genetically modified immature dendritic cells capable of maturation, as well as methods for the production of such cells. In one embodiment, the cells made be produced by a method comprising the steps of providing a population of embryonic stem cells; culturing the embryonic stem cells in the presence of a cytokine or combination of cytokines which brings about differentiation of the embryonic stem cells into dendritic cells; and recovering the dendritic cells from the culture. In a further embodiment, the cells may be genetically modified. | 07-04-2013 |
| 20130171628 | METHOD AND DEVICE FOR ISOLATING CELLS FROM HETEROGENEOUS SOLUTION USING MICROFLUIDIC TRAPPING VORTICES - A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters ≧10 μm flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region. | 07-04-2013 |
| 20130171629 | Extraction Device and Method for Extracting Ingredients from a Biological Sample - An extraction device ( | 07-04-2013 |
| 20130177901 | MARKERS FOR THE PROGNOSIS AND RISK ASSESSMENT OF PREGNANCY-INDUCED HYPERTENSION AND PREECLAMPSIA - The present invention relates to the prognosis and risk assessment in pregnant women to develop pregnancy-induced hypertension and/or preeclampsia by the determination of marker levels. | 07-11-2013 |
| 20130177902 | METHODS AND RELATED DEVICES FOR SINGLE MOLECULE WHOLE GENOME ANALYSIS - Provided are methods of labeling and analyzing features along at least one macromolecule such as a linear biopolymer, including methods of mapping the distribution and frequency of specific sequence motifs or the chemical or proteomic modification state of such sequence motifs along individual unfolded nucleic acid molecules. The present invention also provides methods of identifying signature patterns of sequence or epigenetic variations along such labeled macromolecules for direct massive parallel single molecule level analysis. The present invention also provides systems suitable for high throughput analysis of such labeled macromolecules. | 07-11-2013 |
| 20130177903 | APPARATUS AND METHOD FOR DETECTING DNA DAMAGE - The invention relates to an apparatus and an in-situ method for detecting cellular DNA damage. The invention is particularly suited for use in Comet assays and for automation of such assays. The apparatus comprises a multiwell plate, the plate comprising an array of wells held in fixed relationship with each other and each well having an axis, side walls, and a base and wherein the well is provided with electrode pairs disposed therein; and wherein there is provided means for parallel connection of the electrode pairs to an external voltage supply. | 07-11-2013 |
| 20130183662 | MEANS AND METHODS FOR IDENTIFYING AN INCREASED RISK OF SYSTEMIC LUPUS ERYTHEMATOSUS (SLE) PATIENTS FOR DEVELOPING RENAL MANIFESTATIONS - The present invention relates to means and methods for identifying an increased risk of systemic lupus erythematosus (SLE) patients for developing renal manifestations. The present invention further relates to polypeptides binding to one or more components of neutrophil extracellular traps (NET(s)) and to kits comprising components suitable to carry out the methods provided herein. | 07-18-2013 |
| 20130183663 | LABELED REACTANTS AND THEIR USES - Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions, that include structural components that maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated. | 07-18-2013 |
| 20130183664 | SYSTEMS AND METHODS FOR CONSTRUCTING FREQUENCY LOOKUP TABLES FOR EXPRESSION SYSTEMS - Methods for determining a property that affects expression of polynucleotides are provided. A plurality of polynucleotides each encoding a polypeptide sequence is constructed. A frequency that a sequence element is used in a first polynucleotide is different than in a second polynucleotide. Each polynucleotide is expressed in an expression system to obtain an expression property value thereby constructing a dataset that contains, for each respective polynucleotide, sequence element occurrence in the respective polynucleotide and the measured expression property value of the respective polynucleotide. A model is computed that describes variation in the measured expression property values as a function of a plurality of variables and weights. From the model, a property that affects expression of polynucleotides in the expression system is determined, where the property is an effect that the frequency of occurrence of one or more sequence elements has on the expression property of polynucleotides in the expression system. | 07-18-2013 |
| 20130183665 | SYNTHESIS OF FLUORESCENT NOBLE METAL NANOPARTICLES - A process for the production of fluorescent nanoparticles selected from noble metal, silica or polymer nanoparticles which comprises: 1. A process for the production of fluorescent nanoparticles selected from noble metal or silica nanoparticles which comprises: (1) providing a platform of nanoparticles; (2) covering the surfaces of the nanoparticles to saturation with thiol-terminated polymers by one of the following methods: 1. mixing the nanoparticles with methoxy-(polyethylene glycol)-thiol and biotin-(polyethylene glycol)-thiol; 2. mixing the nanoparticles with fluorescently-labeled methoxy-(polyethylene glycol)-thiol and/or biotin-(polyethylene glycol)-thiol 3. coordinating thiol and biotin thiol to the surfaces of the nanoparticles by a non-covalent bond; and 4. directly conjugating methoxy-thiol and biotin-thiol to the surfaces of the nanoparticles, so that the polymers bind to the surfaces of the nanoparticles as a brush layer via thiol particle coordination of the thiol ends so that the biotin or methoxy ends are free; and (3) homogeneously mixing the resulting biotin nanoparticles with fluorescent avidin or a derivative thereof in proportions such that the final concentration is 1 biotin molecule for every 10 to 1000 avidin molecules in the fluorescent multi-coloured nanoparticle-avidin complexes, each being capable of having a different targeting molecule, and which may be mixed with biotin related targets, and the fluorescent labeled avidin or a derivative thereof being spaced away from the particle surface, thus reducing or removing the potential quenching of the dye. | 07-18-2013 |
| 20130189674 | Methods and Compositions for Enriching Either Target Polynucleotides or Non-Target Polynucleotides from a Mixture of Target and Non-Target Polynucleotides - Compositions and methods are provided for enriching mitochondrial DNA and optionally chloroplast DNA from eukaryotic cells in a simple rapid method that provides greater than 100 fold enrichment. Affinity protein-coated substrate in a buffer is used to efficiently bind chromosomal DNA and thereby remove it from the buffer. Mitochondrial sequencing reads reveal that non-biased sequence selection providing representation of a substantial proportion of mitochondrial DNA in the eukaryotic cells analyzed. | 07-25-2013 |
| 20130203048 | Wound Healing Metakaryotic Stem Cells and Methods of Use Thereof - The invention provides methods of identifying wound healing metakaryotic stem cells, identifying molecules to modulate proliferation and/or migration of metakaryotic stem cells and molecules to treat wound healing disorders, such as blood vessel wound healing disorders, including restenosis. The invention also provides methods of diagnosing and treating wound healing disorders, such as blood vessel wound healing disorders and also of treating wounds by the use of metakaryotic stem cell transplant(s). | 08-08-2013 |
| 20130209994 | FLUORESCENT DYES - The present invention provides dyes, reactive dyes and labeled reagents that may be used in the detection or quantification of desirable target molecules, such as proteins, nucleic acids and cellular organelles. Dyes are provided that may be used free in solution where the binding of the dye to the target molecule provides signal generation. Dyes are also provided that comprise reactive groups that may be used to attach the dyes to probes that will bind to desirable target molecules. The novel dyes of the present invention have been modified to provide beneficial properties. | 08-15-2013 |
| 20130209995 | Laboratory Apparatus for Treating a Sample Reception Section with a Magnetic Tool Device, Magnetic Tool Device, Sample Reception Device for Use with the Magnetic Tool Device and Method for Performing a Work Step on at Least One Fluid Sample Using a Magnetic Field - The invention is related to a laboratory apparatus and method for magnetic treatment of samples, comprising a sample reception device, which has reception zones and engagement zones, which are arranged side by side, and which has a magnetic tool device, which comprises at least one engagement element for at least partly engaging with the at least one engagement zone, wherein the reception device and/or the magnetic tool device or engagement element are arranged movable between a first and a second position for performing a movement which causes the at least one engagement element to at least partly engage the at least one engagement zone, wherein, in the first position, the engagement element does not engage, while in the second position, the engagement element is at least partly engaging with the engagement zone for the magnetic treatment. The invention is also related to the reception device and the magnetic tool device. | 08-15-2013 |
| 20130209997 | COMPOSITIONS FOR STABILIZING DNA, RNA AND PROTEINS IN SALIVA AND OTHER BIOLOGICAL SAMPLES DURING SHIPPING AND STORAGE AT AMBIENT TEMPERATURES - Compositions and methods are disclosed for substantially liquid, gel, suspension, slurry, semisolid and/or colloid storage of biological samples following admixture with the herein disclosed storage composition, permitting substantial recovery of biological activity following storage without refrigeration. In certain embodiments, unfractionated saliva samples may be stored without refrigeration for weeks, months or years in a form that permits recovery of intact DNA following the storage period. | 08-15-2013 |
| 20130217004 | METHODS AND APPARATUS FOR MEASURING ANALYTES - Methods and apparatus relating to FET arrays including large FET arrays for monitoring chemical and/or biological reactions such as nucleic acid sequencing-by-synthesis reactions. Some methods provided herein relate to improving signal (and also signal to noise ratio) from released hydrogen ions during nucleic acid sequencing reactions. | 08-22-2013 |
| 20130217005 | GENERATION OF ANTERIOR FOREGUT ENDODERM FROM PLURIPOTENT CELLS - The invention is directed to in vitro methods of inducing differentiation of anterior foregut endoderm and the enriched populations of anterior foregut endoderm produced by such methods. Such enriched populations are useful for studies of the molecular events that occur during differentiation and for generating cells for cell replacement therapy. | 08-22-2013 |
| 20130217006 | ALGORITHMS FOR SEQUENCE DETERMINATION - The present invention is generally directed to powerful and flexible methods and systems for consensus sequence determination from replicate biomolecule sequence data. It is an object of the present invention to improve the accuracy of consensus biomolecule sequence determination from replicate sequence data by providing methods for assimilating replicate sequence into a final consensus sequence more accurately than any one-pass sequence analysis system. | 08-22-2013 |
| 20130217007 | Recombinant Polymerases With Increased Phototolerance - Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template. | 08-22-2013 |
| 20130217008 | CHIMERIC PROMOTER MOLECULES FOR GENE EXPRESSION IN PROKARYOTES - The present invention provides regulatory polynucleotide molecules isolated from a 16S rDNA for enhanced expression of heterologous genes. The invention further discloses compositions, polynucleotide constructs, transformed host cells containing the regulatory polynucleotide sequences, and methods for preparing and using the same. | 08-22-2013 |
| 20130224733 | METHOD AND APPARATUS FOR SORTING PARTICLES - A multi-channel apparatus for classifying particles according to one or more particle characteristics. The apparatus comprises a plurality of flow cytometry units, each of which is operable to classify particles in a mixture of particles by interrogating a stream of fluid containing the particles with a beam of electromagnetic radiation. The flow cytometry units share an integrated platform comprising at least one of the following: (1) a common supply of particles; (2) a common housing; (3) a common processor for controlling operation of the units; (4) a common processor for receiving and processing information from the units; and (5) a common fluid delivery system. The integrated platform can include a common source of electromagnetic radiation. A method of the invention comprises using a plurality of flow cytometry units sharing the integrated platform to perform a flow kilometric operation, such as analyzing or sorting particles. | 08-29-2013 |
| 20130224735 | Fluorescent Chemical Compounds Having High Selectivity for Double Stranded DNA, and Methods for Their Use - Chemical compounds having a high selectivity for double stranded DNA over RNA and single stranded DNA are disclosed. The chemical compounds are stains that become fluorescent upon illumination and interaction with double stranded DNA, but exhibit reduced or no fluorescence in the absence of double stranded DNA. The compounds can be used in a variety of biological applications to qualitatively or quantitatively assay DNA, even in the presence of RNA. | 08-29-2013 |
| 20130224736 | MICROFLUIDIC DEVICE AND METHOD FOR PROCESSING OF MACROMOLECULES - A microfluidic device and method for enzymatic processing of ultra-long macromolecules is accomplished using a microfluidic device a reaction chamber with a first manifold, a second manifold, and a plurality of reaction channels. Each reaction channel extends from the first manifold to the second manifold. First inlet and outlet channels fill the reaction channels via the manifolds with one or more macromolecule containers suspended in a first carrier fluid. The first inlet and outlet channels are configured such that a flow is guided through the reaction channels, and an enzymatic reagent is fed to the reaction chamber essentially without displacing the macromolecule containers trapped in the reaction channels. The second set of inlets and outlets are configured such that a flow established from the second inlet to the second outlet is guided through at least one of the manifolds and bypasses the reaction channels. | 08-29-2013 |
| 20130224737 | Method For Determining The Production Of Reactive Oxygen Species In A Cellular Population - The invention relates to a method for determining the production of reactive oxygen species in a cellular population. The invention also relates to a method for determining the need for an antioxidant treatment of a male subject, and to a method for identifying a substance that can reduce the reactive oxygen species in a cellular population. | 08-29-2013 |
| 20130236885 | MULTIPLE SEPARATION DEVICE AND METHOD OF SEPARATING BLOOD CANCER CELL - The present inventive concept provides a multiple separation device and a method of separating a blood cancer cell using the same. In the device and the method, a blood sample is put in a fine channel and then cancer cells can be separated according to the type of cancer by controlling a flow velocity of the blood or a magnetic force of ferromagnetic pattern. | 09-12-2013 |
| 20130236886 | Phage Phi 29 DNA Polymerase Chimera - A DNA polymerase chimera comprising an amino-terminal (N-terminal) region encoding a φ29 type DNA polymerase and a carboxyl-terminal (C-terminal) region comprising at least one HhH domain which are bound by a connecting amino acid sequence is disclosed along with and the use thereof for replicating, amplifying or sequencing a template DNA. Also disclosed is a method for replicating, amplifying or sequencing a deoxyribonucleic acid with a DNA polymerase chimera and kits for carrying out the methods. | 09-12-2013 |
| 20130236887 | Method and Apparatus for Measuring Analyte Transport Across Barriers - The present invention includes a method and apparatus for measuring the transport of analytes through a cell barrier. | 09-12-2013 |
| 20130236888 | ENZYME SUBSTRATE COMPRISING A FUNCTIONAL DYE AND ASSOCIATED TECHNOLOGY AND METHODS - Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and/or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease. | 09-12-2013 |
| 20130244230 | METHODS AND MEANS FOR CHARACTERIZING ANTIBIOTIC RESISTANCE IN MICROORGANISMS - The present invention relates to a method for characterizing the antibiotic resistance of a microorganism, said method comprising the steps of (a) providing a reference mass spectrum of an antimicrobial compound, its enzymatic modification product, its molecular target, or of a substrate compound of a its modifying enzyme; (b) exposing a microorganism, a cell lysate thereof, or a growth medium supernatant thereof, to said antimicrobial compound or said substrate compound in aqueous liquid to thereby provide an exposed sample; (c) acquiring a mass spectrum of the exposed sample; (d) comparing the mass spectrum acquired in step c) with the reference mass spectrum of step (a), and (e) determining from said comparison whether modification of said antimicrobial compound, its modification product or its molecular target or of said substrate has occurred following said exposure, and establishing that said microorganism is potentially resistant to said antimicrobial compound when said modification is observed. | 09-19-2013 |
| 20130244231 | NOVEL EXPRESSION VECTOR - Disclosed are a novel expression vector for efficient expression of recombinant proteins in mammalian cells, a mammalian cell transformed with the vector, and a method for production of the mammalian cell. The expression vector includes a gene expression regulatory site, and a gene encoding the protein downstream thereof, and an internal ribosome entry site further downstream thereof, and a gene encoding a glutamine synthetase further downstream thereof. | 09-19-2013 |
| 20130252235 | Mobility Controlled Single Macromolecule in Nanofluidic System and its Application as Macromolecule Sequencer - In one embodiment, the present application discloses a microfluidic device comprising at least one nanochannel, in part, configured to receive a macromolecule, at least one external electrodes configured with the nanochannel, that is further configured to provide contact of the macromolecule with an enzyme to cleave the macromolecule to form a fragment of the macromolecule. Also disclosed are methods of using the microfluidic device for detecting and identifying a property of the fragment of the macromolecule. | 09-26-2013 |
| 20130252236 | TUMOR STEM CELLS - Tumor stem cells can be obtained by culturing a tumor cell population, and exposing the cultured tumor cell population to free radicals. In certain embodiments, the free radical agent can be a nitric oxide (NO) donor. In one embodiment, the free radical agent can be Diethylenetriamine NONOate (DETA NONOate) or agents that constitutively increase cellular nitric oxide, such as phosphodiesterase inhibitors or L-arginine, or agents that increase NO synthase in the population. The methods can further include inducing stem cells present in the population to expand and/or inducing dedifferentiation of tumor cells into tumor stem cells. Additionally, the present invention provides methods of selecting stem cells from a tumor cell population. Another aspect provides methods of screening for anti-tumor stem cell teherapeutic compounds by providing high nitric oxide (HNO) tumor cells, exposing the HNO cells to at least one compound, assessing one or more indicators of HNO cell health and determining toxicity of the compound to HNO tumor cells. | 09-26-2013 |
| 20130252237 | CYTOMETRY SYSTEM WITH INTERFEROMETRIC MEASUREMENT - This disclosure concerns methods and apparatus for interferometric spectroscopic measurements of particles with higher signal to noise ratio utilizing an infrared light beam that is split into two beams. At least one beam may be directed through a measurement volume containing a sample including a medium. The two beams may then be recombined and measured by a detector. The phase differential between the two beams may be selected to provide destructive interference when no particle is present in the measurement volume. A sample including medium with a particle is introduced to the measurement volume and the detected change resulting from at least one of resonant mid-infrared absorption, non-resonant mid-infrared absorption, and scattering by the particle may be used to determine a property of the particle. A wide range of properties of particles may be determined, wherein the particles may include living cells. | 09-26-2013 |
| 20130260371 | APPARATUS AND METHOD FOR MOLECULAR SEPARATION, PURIFICATION, AND SENSING - Described are devices and methods for forming one or more nanomembranes including electroactive nanomembranes within a nanowell or nanotube, or combinations thereof, in a support material. Nanopores/nanochannels can be formed by the electroactive nanomembrane within corresponding nanowells. The electroactive nanomembrane is capable of controllably altering a dimension, a composition, and/or a variety of properties in response to electrical stimuli. Various embodiments also include devices/systems and methods for using the nanomembrane-containing devices for molecular separation, purification, sensing, etc. | 10-03-2013 |
| 20130260372 | INTEGRATED OPTOELECTRONIC READ HEAD AND FLUIDIC CARTRIDGE USEFUL FOR NUCLEIC ACID SEQUENCING - A detection apparatus having a read head including a plurality of microfluorometers positioned to simultaneously acquire a plurality of the wide-field images in a common plane; and (b) a translation stage configured to move the read head along a substrate that is in the common plane. The substrate can be a flow cell that is included in a cartridge, the cartridge also including a housing for (i) a sample reservoir; (ii) a fluidic line between the sample reservoir and the flow cell; (iii) several reagent reservoirs in fluid communication with the flow cell, (iv) at least one valve configured to mediate fluid communication between the reservoirs and the flow cell; and (v) at least one pressure source configured to move liquids from the reservoirs to the flow cell. The detection apparatus and cartridge can be used together or independent of each other. | 10-03-2013 |
| 20130266935 | FOCUSING CHAMBER - Aspects of the invention relate to devices and methods of use thereof for concentrating, positioning and/or manipulating agents within a fluid, including but not limited to genomic DNA. | 10-10-2013 |
| 20130273526 | DNA Polymerases Having Improved Labeled Nucleotide Incorporation Properties - The present invention relates to mutant DNA polymerases that exhibit reduced discrimination against labeled nucleotides into polynucleotides. The DNA polymerases of the invention have at least one mutation in the nucleotide label interaction region of the enzyme such the mutation results in reduced discrimination against labeled nucleotides. The nucleotide label interaction regions are located at portions of the O-helix, the K-helix, and the inter O-P helical loop of Taq DNA polymerase or analogous positions in other DNA polymerases. | 10-17-2013 |
| 20130273527 | METHOD FOR INDUCING CELL SENESCENCE BY RECOMBINANT INTERFERON WITH ALTERED SPATIAL CONFIGURATION - This invention provides a method for inducing cell senescence by recombinant interferon with altered spatial configuration, wherein the interferon is encoded by a nucleotide sequence having the sequence of SEQ ID NO.2. | 10-17-2013 |
| 20130273528 | DEVICE FOR PERFORMING A DIAGNOSTIC TEST AND METHODS FOR USE THEREOF - Assay cassettes and testing devices that can be used to provide rapid, accurate, affordable, laboratory-quality testing at the point of care. Such assay cassettes and testing devices are designed to provide rapid, quantitative test results in a point-of-care setting or the like. Likewise, such assay cassettes and testing devices may eliminate or replace expensive, centralized clinical testing equipment and technical personnel. Such testing device may include automated data reporting and decision support. Methods for performing point of care diagnostic tests are also disclosed. | 10-17-2013 |
| 20130273529 | Identifying and Correcting An Allelic Ladder Signal For DNA Analysis - A method for determining an allelic ladder signal for DNA analysis includes obtaining a measured allelic ladder signal for an allelic ladder substance, which includes a plurality of fragments, obtaining a reference set of expected fragment sizes of fragments of the ladder substance, and generating a signal identifying whether a peak for a fragment size of the measured ladder signal is a true peak of the ladder substance based on the reference set of expected fragment sizes, wherein the allelic ladder signal for DNA analysis includes the true peaks identified in the signal. | 10-17-2013 |
| 20130273530 | ANALYTICAL DEVICE AND ANALYTICAL METHOD - The present invention provides a technique capable of simply analyzing a target to be analyzed. An analytical device of the present invention includes a basal plate; a nucleic acid element; and a detection section of detecting a signal. The nucleic acid element and the detection section are arranged on the basal plate. The nucleic acid element includes a first nucleic acid molecule and a second nucleic acid molecule. The first nucleic acid molecule is a nucleic acid molecule capable of binding to a target. The second nucleic acid molecule is a nucleic acid molecule capable of binding to streptavidin. When the target does not bind to the first nucleic acid molecule, a binding capacity of the second nucleic acid molecule to the streptavidin is inactivated. When the target binds to the first nucleic acid molecule, a binding capacity of the second nucleic acid molecule to the streptavidin is activated. The detection section detects binding between the second nucleic acid molecule and the streptavidin. The target is bound to the first nucleic acid molecule, so that the streptavidin is bound to the second nucleic acid molecule. Thus, the target can be analyzed through detecting the binding between the second nucleic acid molecule and the streptavidin using the detection device. | 10-17-2013 |
| 20130280700 | FOUR-COLOR DNA SEQUENCING BY SYNTHESIS USING CLEAVABLE FLUORESCENT NUCLEOTIDE REVERSIBLE TERMINATORS - This invention provides a process for sequencing single-stranded DNA employing modified nucleotides. | 10-24-2013 |
| 20130280701 | System and Method for High-Content Oncology Assay - The present invention provides an apparatus, system, method and computer program and computer program product for analyzing cellular samples. One embodiment of the apparatus and method provides a multiparameter assay that provides information with respect to cell proliferation, cell cycling and cell death. The multiparameter assay is particularly useful for assessing and screening candidate compounds for anti-cancer utility. | 10-24-2013 |
| 20130280702 | ALTERNATIVE NUCLEOTIDE FLOWS IN SEQUENCING-BY-SYNTHESIS METHODS - A method for sequencing a polynucleotide strand by using sequencing-by-synthesis techniques. To address the problem of incomplete extension (IE) and/or carry forward (CF) errors that can occur in sequencing-by-synthesis reactions, an alternative flow ordering of dNTPs is used. In contrast to conventional flow orderings, the dNTPs are flowed in an ordering that is not a continuous repeat of an ordering of the four different dNTPs. This alternate flow ordering may reduce the loss of phasic synchrony in the population of template polynucleotide strands that result from IE and/or CF errors. | 10-24-2013 |
| 20130288234 | DEVICE FOR PREPARING A SAMPLE - The invention provides a device for preparing a fluid sample, including but not limited to a sample comprising genomic DNA. | 10-31-2013 |
| 20130288235 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 10-31-2013 |
| 20130295558 | METHOD OF DETECTING ETHYLATED THYMIDINE DNA ADDUCTS - A method of analyzing ethylated thymidine DNA adducts is disclosed. The method comprises the steps of: providing leukocyte DNA; adding at least one isotope-labeled internal standard and a plurality of enzymes to the leukocyte DNA, and hydrolyzing the leukocyte DNA into a plurality of nucleosides; using a solid-phase extraction column to extract the plurality of nucleosides; and using a stable isotope dilution nanoflow liquid chromatography-nanospray ionization tandem mass spectrometry to detect and quantify at least one ethylated thymidine DNA adduct in the plurality of extracted nucleosides. | 11-07-2013 |
| 20130295559 | POLYMERASE - The present invention relates to an engineered polymerase with an expanded substrate range characterised in that the polymerase is capable of incorporating an enhanced occurrence of detection agent-labelled nucleotide analogue into nucleic acid synthesised by that engineered polymerase as compared with the wild type polymerase from which it is derived. | 11-07-2013 |
| 20130295560 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 11-07-2013 |
| 20130302791 | DEVICE FOR ISOLATING AN ANALYTE FROM A SAMPLE, AND METHODS OF USE - A device for extraction or isolation of an analyte, such as a nucleic acid, a protein, or a cell, from a sample, and in particular from a biological sample is described. Methods of using the device are also described. | 11-14-2013 |
| 20130302792 | CALIBRATIONS AND CONTROLS FOR DROPLET-BASED ASSAYS - System, including methods and apparatus, for performing droplet-based assays that are controlled and/or calibrated using signals detected from droplets. | 11-14-2013 |
| 20130302793 | METHOD OF MAKING AND USING A LIBRARY OF BIOLOGICAL MATERIAL - Biologic information is obtained concerning a member of a population by obtaining a sample of placental tissue from the member, storing the sample without embedding it in an embedding medium, retrieving from storage the sample associated with the member and thereafter analyzing it for biologic information. Storage may be in a fixative such as formalin or a formalin substitute. When a tissue sample from more than one member is collected, a library is created that may be used for a variety of purposes, including reducing the incidence of medical malpractice claims, identification of members such as paternity testing or suspect identification, pharmaceutical development and epidemiological surveys and research. | 11-14-2013 |
| 20130309659 | CELL CULTURING FORMULATION AND CULTURING AND QUANTIFICATION METHOD OF CD140B+ CELLS THEREOF - The present invention discloses a cell culturing formulation and a culturing and quantification method of CD140b+ cells thereof. The cell culturing formulation is applicable for inducing the growth of the CD140b+ cells in peripheral blood. The cell culturing formulation comprises a culturing medium, a serum, a mixed additive and a defined factor. Wherein, concentrations of the culturing medium, the serum, the mixed additive and the defined factor are 59˜98% (v/v), 0.1˜20% (v/v), 1˜10% (v/v) and 10 | 11-21-2013 |
| 20130309660 | METHODS OF CHARACTERIZING, DETERMINING SIMILARITY, PREDICTING CORRELATION BETWEEN AND REPRESENTING SEQUENCES AND SYSTEMS AND INDICATORS THEREFOR - A computer implemented method for characterizing one or more sequences by generating index values representing portions of the sequences and finding characterizing index values based on a comparison of the index values. The index values may be obtained by applying one or more mask over each sequence. The modified masks may have associated weightings and index values obtained using modified masks may be retained in the index only if the weightings are above a threshold value. Characterising index values may also be assessed for for their degree of uniqueness. Characterizing indexes may be used for predicting correlation between a sample sequence and one or more reference sequences. Biological monitoring systems utilising the characterizing index values are also disclosed. A biological indicator may be generatgenerated using one or more characterizing index values obtained by the above method and be used to produce an indicator that undergoes a property change in the presence of the one or more sequence. | 11-21-2013 |
| 20130309661 | FREE SOLUTION MEASUREMENT OF MOLECULAR INTERACTIONS BY BACKSCATTERING INTERFEROMETRY - Disclosed are methods, systems, and apparatuses for the free solution measurement of molecular interactions by backscattering interferometry. In one aspect, the invention relates to method and systems for detecting molecular interaction between analytes in free-solution wherein the analytes are label-free and detection is performed by back-scattering interferometry. Also disclosed are label-free, free-solution, and/or real-time measurements of characteristic properties and/or chemical events using the disclosed techniques. The disclosed methods can have very low detection limits and/or very low sample volume requirements. Also disclosed are various biosensor applications of the disclosed techniques. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention. | 11-21-2013 |
| 20130309662 | METHOD OF SEPARATING TARGET CELL IN BIOLOGICAL SAMPLE - Provided is a method of separating a rare cell from a sample comprising incubating a sample comprising a rare cell and a second cell with a particle comprising a moiety capable of binding to the rare cell to form a complex comprising the particle and the rare cell; applying the mixture to a medium having a density gradient; and centrifuging the mixture to separate the complex from the second cell. | 11-21-2013 |
| 20130316335 | SAMPLE HANDLING - The invention provides containers and methods of use for the storage, transportation and preparation of samples, such as DNA samples for analysis. The container is pre-provided with the reagents in sealed chambers. The sample can be introduced and the container manipulated to release the reagents, provide the necessary conditions and give a fully prepared sample. The container can then be engaged with an analysis device to identify characteristics of the sample or perform other operations thereon. | 11-28-2013 |
| 20130316336 | ANALYZER - Provided is an analyzer capable of reducing the amount of wasted reagents and shortening time required for solution sending, thus increasing throughput for analysis. A microsyringe sucks a minimum required amount of reagent that is substantially the same amount of capacity of a flow cell to a sampling nozzle. Then, the sampling nozzle is inserted into an injection port of the flow cell, and the reagent is injected into the flow cell by driving the microsyringe. The inside of the sampling nozzle is cleaned by moving the sampling nozzle to the cleaning tank and ejecting cleaning water from the sampling nozzle, and the outside of the sampling nozzle is cleaned by spraying cleaning water from an inner wall of the cleaning tank. | 11-28-2013 |
| 20130323721 | HEPATOCYTES FOR IN VITRO GENOTOXICITY TESTS - The invention relates to a method for carrying out genotoxicity tests of chemical, biological and physical active substances or agents with the aid of cell culture systems of proliferating physiologically active liver cells. | 12-05-2013 |
| 20130323722 | Methods for High Fidelity Production of Long Nucleic Acid Molecules - In a method for generating a long nucleic acid molecule, nucleic acids immobilized on a surface and having overlapping complementary sequences is released into solution. The overlapping complementary sequences are hybridized to form hybridized nucleic acids, followed by extension or ligation of the hybridized nucleic acids to synthesize the long nucleic acid molecule. The nucleic acids may comprise first and second series of nucleic acids having redundant overlapping sequences, wherein nucleic acids from the first and second series are complementary to each other. The complementary nucleic acids are hybridized to form the hybridized nucleic acids. The generated long nucleic acid molecule may have a predetermined sequence element, and it may be introduced into a system wherein the predetermined sequence element is required for replication, such that replication of the synthesized long nucleic acid molecule is indicative of the presence of the predetermined sequence element in the long nucleic acid molecule. | 12-05-2013 |
| 20130323723 | SOLID SUPPORT AND METHOD OF RECOVERING BIOLOGICAL MATERIAL THEREFROM - The present invention relates to solid supports that are used for the storage and further processing of biological materials. The invention is particularly concerned with solid supports which have at least one surface coated with a chemical mixture that enhances the recovery of the biological material from the support. Methods of preparing and using the solid supports are also described. | 12-05-2013 |
| 20130330717 | METHOD FOR INTRODUCING A POLYNUCLEOTIDE INTO NON-ADHESIVELY GROWING PLANT CELLS - The present invention relates to a method for introducing a polynucleotide into non-adhesively growing plant cells, comprising the following steps: providing a solid support having immobilized thereto the polynucleotide in dry state; contacting the plant cells with the polynucleotide on the solid support so as to obtain transformed plant cells; and optionally washing the plant cells. | 12-12-2013 |
| 20130330718 | PROTEINS THAT EFFICIENTLY GENERATE SINGLET OXYGEN - The present invention provides miniSOG proteins, polynucleotides, and methods of use. When expressed in a bacterial or mammalian cell, miniSOG proteins spontaneously incorporate flavin mononucleotide and produce fluorescence and singlet oxygen upon excitation. Uses include optical and electron microscope imaging, in vivo imaging, detection and localization of protein-protein interactions, and photoablation. | 12-12-2013 |
| 20130337440 | METHODS FOR MICROVESICLE ISOLATION AND SELECTIVE REMOVAL - The invention relates to compositions and methods for isolation of microvesicles produced by mammalian cells. These microvesicles, known as extracellular microvesicles or circulating microvesicles, are isolated from sample materials such as body fluids, or from cell culture media that has been used to culture and maintain mammalian cells in vitro. The isolation of microvesicles as described herein results in purification and concentration of the microvesicles. | 12-19-2013 |
| 20130337441 | COMBINED HISTOLOGICAL STAIN - The present invention relates to methods of visualizing targets in histological samples, e.g. biopsy samples, wherein the methods comprise staining of the sample with (i) one or more target specific immunochemical stains, and (ii) a histological stain for specific tissue components e.g. iron, mucins glycogen, amyloid, nucleic acids, etc., e.g. hematoxilyn and/or eosin stains or the like, that is used to enhance contrast in the microscopic image of a tissue sample, highlight morphologic structures in the sample for viewing, define and examine tissues, cell populations, or organelles within individual cells. Methods may further comprise evaluation of expression of one or more targets in the sample. The disclosed methods are useful for medical diagnostics. | 12-19-2013 |
| 20130344480 | Method for Treating a Blood Component Containing Sample - Provided is a treatment method for damaging an erythrocyte and a leukocyte while suppressing damage to cells other than blood cells present in blood. In an embodiment, the disclosure relates to a method for treating a sample containing blood components, the method including mixing a sample containing blood components with a surfactant A, where the surfactant A is a nonionic surfactant represented by General formula R | 12-26-2013 |
| 20140004507 | Microfluidic System Having Monolithic Nanoplasmonic Structures | 01-02-2014 |
| 20140011192 | PARTICLE COMPLEX AND METHOD OF ISOLATING TARGET CELL - A particle complex comprising a particle; a cleavable linker bound to a surface of the particle; and a macromolecule bound to the cleavable linker, wherein the macromolecule specifically binds to a surface marker of a target cell, and a method of isolating a target cell comprising same. | 01-09-2014 |
| 20140011193 | APTAMER-BASED LATERAL FLOW ASSAY AND ASSOCIATED METHODS - Methods, assays, and products for the detection of analytes in a sample are provided. In one aspect, for example, a device for detecting an analyte in a sample can include a fluid transfer membrane further including a sample input region operable to receive a liquid sample, a reagent region including a first split aptamer segment, a second split aptamer segment, and a detection marker, where the first and second split aptamers are operable to ligate in the presence of the analyte. The detection marker is operable to bind to the second split aptamer. The device can further include a test region having an immobilized binding reagent operable to bind to the first split aptamer segment such that the detection marker is held in the test region when the first split aptamer segment is ligated to the second split aptamer segment due to the analyte being present in the sample. | 01-09-2014 |
| 20140017673 | METHOD FOR ENUMERATING EUKARYOTIC CELL MICRONUCLEI WITH AN EMPHASIS ON SIMULTANEOUSLY ACQUIRING CYTOTOXICITY AND MODE OF ACTION INFORMATION - The present invention relates a method for the enumeration of eukaryotic cell micronuclei, while simultaneously acquiring cytotoxicity and mode of action information. The method utilizes differential labeling of chromatin from dead and dying cells to distinguish the chromatin from micronuclei, nuclei, and metaphase chromosomes, and differential labeling of metaphase events to provide additional information regarding cytotoxicity and genotoxic modes of action. Counting of micronuclei events relative to the number of nuclei and quantifying perturbations to the proportion of metaphase events can be used to assess the DNA-damaging potential of a chemical agent, the DNA-damaging potential of a physical agent, the effects of an agent which can modify endogenously-induced DNA damage, the effects of an agent which can modify exogenously-induced DNA damage, and genotoxic mode of action. | 01-16-2014 |
| 20140017674 | Methods and Compositions for Performing Analytical Operations - Methods for performing analytical reactions and compositions for use in such methods, where the methods have reduced signal levels deriving from non-specific adsorption of detected reagents to other components of the analytical method, e.g., other reagents, solid phase components, vessels, etc. | 01-16-2014 |
| 20140017675 | ULTRA-HIGH-SENSITIVE ASSAY OF PROTEIN AND NUCLEIC ACID AND KIT, AND NOVEL ENZYME SUBSTRATE - Provided is a ultra-high-sensitivity assay in which the assay can be made on a commonly used assay apparatus such as an absorptiometer and a plate reader or with naked eyes. The high-sensitivity assay in which the assay can be made on a commonly used assay apparatus or with naked eyes can be provided by combining an enzyme cycling method using thio-NAD(P) as a coenzyme, a labeling enzyme and a substrate for the labeling enzyme optimally, and by amplifying thio-NAD(P)H, which is a signaling substance, exponentially and then quantifying the thio-NAD(P)H colorimetrically. | 01-16-2014 |
| 20140024022 | CELL TREATMENT SOLUTION AND METHOD OF PREPARING STAINED CELL SUSPENSION FOR A MEASUREMENT OF NUCLEAR DNA BY FLOW CYTOMETRY - A cell treatment solution and a method that is used for preparing a stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry. The cell treatment solution may include a surfactant, RNase, and a fluorescent dye. The surfactant may include, for example, a non-ionic surfactant, a zwitterionic surfactant, an anionic surfactant, and/or a cationic surfactant. In one method of the presently disclosed subject matter, stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry is prepared. The method may include adding a tissue sample to a cell treatment solution including a surfactant, RNase, and fluorescent dye, disaggregating the tissue sample, and filtering the disaggregated tissue sample. Another method of the presently disclosed subject matter includes disaggregating a tissue sample, preparing cell suspension by filtering the disaggregated tissue sample, and adding a cell treatment solution including a surfactant, RNase, and fluorescent dye. | 01-23-2014 |
| 20140024023 | DROPLET GENERATION SYSTEM WITH FEATURES FOR SAMPLE POSITIONING - System, including methods and apparatus, for forming droplets of an emulsion. The system may include a channel junction at which a stream of sample fluid is divided into droplets by a dividing flow of carrier fluid. The system also may include one or more features configured to position sample fluid for reduced contact between the sample fluid and one or more surface regions of the channel junction, which may improve the consistency of droplet formation. In exemplary embodiments, sample fluid may be positioned by a step member produced by an increase in channel depth, and/or by directing flow of carrier fluid to form a barrier layer between sample fluid and a wall region, such as a ceiling region or floor region, of a channel network. | 01-23-2014 |
| 20140030704 | Asymmetric Adapter Library Construction - The present invention provides methods and compositions for asymmetrically tagging a nucleic acid fragment using asymmetric adapters. | 01-30-2014 |
| 20140030705 | SYSTEMS AND METHODS FOR ASSESSING BIOMOLECULE CHARACTERISTICS - Provided are methods and systems for assessing the presence and extent of damage on a polynucleotide. The methods include incorporating a label at the site of the damage and imaging the label to determine the presence and extent of the damage. The systems include devices capable of performing damage assessment on single molecules. | 01-30-2014 |
| 20140030706 | METHODS OF IDENTIFYING A CELLULAR NASCENT RNA TRANSCRIPT - Methods and compositions for identifying a cellular nascent RNA transcript are provided. | 01-30-2014 |
| 20140030707 | SMALL RNA-DEPENDENT TRANSLATIONAL REGULATORY SYSTEM IN CELL OR ARTIFICIAL CELL MODEL - An object of the present invention is to construct an mRNA which specifically responds to a short RNA sequence and can activate, repress, and regulate the translation of the desired gene, and to construct an artificial cell model system using a liposome comprising the mRNA and a cell-free translational system encapsulated therein. The present invention provides: an mRNA comprising a target RNA-binding site located immediately 5′ to the ribosome-binding site, and a nucleotide sequence located 5′ to the target RNA-binding site, the nucleotide sequence being complementary to the ribosome-binding site; an mRNA comprising a small RNA-binding site located 3′ to the start codon, and a nucleotide sequence located 3′ to the small RNA-binding site, the nucleotide sequence encoding a protein; and a liposome comprising any of these mRNAs encapsulated therein. | 01-30-2014 |
| 20140030708 | TRANSLATION-COUPLING SYSTEMS - Disclosed are systems and methods for coupling translation of a target gene to a detectable response gene. A version of the invention includes a translation-coupling cassette. The translation-coupling cassette includes a target gene, a response gene, a response-gene translation control element, and a secondary structure-forming sequence that reversibly forms a secondary structure masking the response-gene translation control element. Masking of the response-gene translation control element inhibits translation of the response gene. Full translation of the target gene results in unfolding of the secondary structure and consequent translation of the response gene. Translation of the target gene is determined by detecting presence of the response-gene protein product. The invention further includes RNA transcripts of the translation-coupling cassettes, vectors comprising the translation-coupling cassettes, hosts comprising the translation-coupling cassettes, methods of using the translation-coupling cassettes, and gene products produced with the translation-coupling cassettes. | 01-30-2014 |
| 20140038176 | METHOD OF DETECTING NUCLEIC ACIDS, METHOD OF OPTICALLY OBSERVING SAMPLE AND FLUORESCENT SUBSTANCE - [Object] A method of detecting nucleic acids easily without requiring complicated operations such as mixing of liquids and cleaning within micro-scale flow channels. | 02-06-2014 |
| 20140038177 | Brown Fat Cell Compositions and Methods - Methods of developing and using cell lines, such as stem cell lines, for therapeutic or cosmetic use. In one embodiment the cell lines are used to treat a wide range of degenerative and metabolic disorders including, but not limited to, obesity, diabetes, hypertension, and cardiac deficiency. Also described are methods of using such cell lines to screen for compounds that play a role in regulating a variety of processes. | 02-06-2014 |
| 20140038178 | CIS Reactive Oxygen Quenchers Integrated into Linkers - The present invention provides methods and compositions for performing illuminated reactions, particularly sequencing reactions, while mitigating and/or preventing photodamage to reactants that can result from prolonged illumination. In particular, the invention provides methods and compositions for incorporating photoprotective agents into conjugates comprising reporter molecules and nucleoside polyphosphates. | 02-06-2014 |
| 20140038179 | REAGENT AND REAGENT KIT FOR ANALYSIS OF IMMATURE LEUKOCYTE - The present invention provides a reagent for analysis of immature leukocytes comprising:
| 02-06-2014 |
| 20140045175 | Hydroxymethyl Linkers For Labeling Nucleotides - The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses. | 02-13-2014 |
| 20140045176 | METHOD FOR CONCENTRATING CELLS THAT ARE GENETICALLY ALTERED BY NUCLEASES - The present invention relates to a reporter construct and method for identifying or enriching the cells, wherein a specific endogenous nucleotide sequence is cleaved by a specific nuclease or modified by such cleavage; a host cell comprising the reporter construct; and a system for monitoring a nuclease activity. The reporter system of the present invention is simple and non-invasive, and allows for an efficient enrichment of the gene-modified cells. Therefore, the present invention will promote the application of a nuclease in the field of gene therapy and genetic engineering as well as basic research. | 02-13-2014 |
| 20140051068 | CONTROL OF DNA MOVEMENT IN A NANOPORE AT ONE NUCLEOTIDE PRECISION BY A PROCESSIVE ENZYME - The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore. Of particular note is the stability of the system in a saline medium and to detect individual nucleotide bases in a polynucleotide in real time and which may be used to sequence DNA for many hours without change of reagents. The invention is of particular use in the fields of forensic biology, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof. | 02-20-2014 |
| 20140051069 | ENZYME-PORE CONSTRUCTS - The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing. | 02-20-2014 |
| 20140051070 | STREPTAVIDIN-COUPLED MAGNETIC PARTICLES AND MANUFACTURING METHOD FOR SAME - The present invention provides a streptavidin-coupled magnetic particle with high biotin-binding capacity, and a manufacturing method thereof. The streptavidin-coupled magnetic particle has a structure in which streptavidins are cross-linked with each other on a magnetic particle. A method for manufacturing the streptavidin-coupled magnetic particle includes the steps of: | 02-20-2014 |
| 20140051071 | BLOOD ANALYZER, BLOOD ANALYSIS METHOD, AND COMPUTER PROGRAM PRODUCT - A blood analyzer, a blood analysis method, and a computer program that can distinguishably detect abnormal lymphocytes from blasts and atypical lymphocytes are provided. A blood analyzer ( | 02-20-2014 |
| 20140057250 | Compositions Comprising Human Embryonic Stem Cells and Their Derivatives, Methods of Use, and Methods of Preparation - The present invention relates to a pharmaceutical composition comprising of preparations of human embryonic stem (hES) cells and their derivatives and methods for their transplantation into the human body, wherein transplantation results in the clinical reversal of symptoms, cure, stabilization or arrest of degeneration of a wide variety of presently incurable and terminal medical conditions, diseases and disorders. The invention further relates to novel processes of preparing novel stem cell lines which are free of animal products, feeder cells, growth factors, leukaemia inhibitory factor, supplementary mineral combinations, amino acid supplements, vitamin supplements, fibroblast growth factor, membrane associated steel factor, soluble steel factor and conditioned media. This invention further relates to the isolation, culture, maintenance, expansion, differentiation, storage, and preservation of such stem cells. | 02-27-2014 |
| 20140065604 | METHODS AND SYSTEMS FOR SEQUENCING LONG NUCLEIC ACIDS - The present invention provides methods and systems for sequencing long nucleic acid fragments. | 03-06-2014 |
| 20140065605 | METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS - The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. Certain methods are provided that include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Other methods are provided that include a Staudinger ligation between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent comprising a substituted triarylphosphine attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures. | 03-06-2014 |
| 20140065606 | Compositions, Apparatus and Methods for Monitoring Biomarkers - A single device to collect, transfer, and measure salivary nitric oxide analyte and metabolite, nitrite, a biomarker for nitric oxide, as well as, a method to assess the effects of diet and exercise on changing an individual's nitric oxide status and health. | 03-06-2014 |
| 20140065607 | PHOSPHOLINK NUCLEOTIDES FOR SEQUENCING APPLICATIONS - The present invention provides labeled phospholink, nucleotides that can be used in place of naturally occurring nucleotide triphosphates or other analogs in template directed nucleic acid synthesis reactions and other nucleic acid reactions and various analyses based thereon, including DNA sequencing, single base identification, hybridization assays, and others. | 03-06-2014 |
| 20140065608 | METHOD FOR THE ENUMERATION OF MAMMALIAN MICRONUCLEATED ERYTHROCYTE POPULATIONS, WHILE DISTINGUISHING PLATELETS AND/OR PLATELET-ASSOCIATED AGGREGATES - A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures. | 03-06-2014 |
| 20140080122 | Optical Nanosensors Comprising Photoluminescent Nanostructures - Systems and methods related to optical nanosensors comprising photoluminescent nanostructures are generally described. Generally, the nanosensors comprise a photoluminescent nanostructure and a polymer that interacts with the photoluminescent nanostructure. In some cases, the interaction between the polymer and the nanostructure can be non-covalent (e.g., via van der Waals interactions). The nanosensors comprising a polymer and a photoluminescent nanostructure may be particularly useful in determining the presence and/or concentration of relatively small molecules, in some embodiments. In addition, in some instances the nanosensors may be capable of determining relatively low concentrations of analytes, in some cases determining as little as a single molecule. In some embodiments, the interaction between the analyte and the nanosensor (e.g., between the analyte and the photoluminescent nanostructure) can be reversible, which may allow, for example, for the reuse of a nanosensor after it has been exposed to an analyte. | 03-20-2014 |
| 20140080123 | METHODS OF USING A SERUM RESPONSE FACTOR ISOFORM - The present invention encompasses methods of administering SRFΔ3. Administering SRFΔ3 may reduce proliferation of cancer cells, and modulate activity of promoters regulated by serum response factor. | 03-20-2014 |
| 20140087370 | SAMPLE TREATMENT DEVICE, SAMPLE TREATMENT METHOD, AND REACTION CONTAINER FOR USE THEREIN - Reaction containers ( | 03-27-2014 |
| 20140093869 | DESIGN AND SYNTHESIS OF CLEAVABLE FLUORESCENT NUCLEOTIDES AS REVERSIBLE TERMINATORS FOR DNA SEQUENCING BY SYNTHESIS - This invention provides novel azido linkers for deoxynucleotide analogues having a detectable marker attached thereto. | 04-03-2014 |
| 20140093870 | SINGLE MOLECULE IMAGING TECHNIQUES TO AID CRYSTALLIZATION - This invention relates to a method to rapidly find crystallization conditions for a biomolecule in a desired conformation using single-molecule, fluorescent resonance energy transfer (smFRET) imaging techniques. The method provides significant cost and time advantages over the empricial exploration for crystallization conditions. | 04-03-2014 |
| 20140099632 | PREPARATION AND USE OF NUCLEATED RED BLOOD CELL SIMULATING PARTICLES AND HEMATOLOGY CONTROL MIXTURES - The present disclosure provides a nucleated red blood cell simulating particle, which may be leukocytes bound to a fluorescent-staining inhibitor capable of stably binding to the nucleus or a nucleic acid in a cell so as to reduce the binding capacity of the particles to a fluorescent dye during their detection. The present disclosure also provides a method for preparing nucleated red blood cell simulating particles, including the following steps: (a) obtaining purified leukocytes; (b) suspending the leukocytes in a cell treatment solution containing a fluorescent-staining inhibitor which stably binds to the nucleus or a nucleic acid in a cell, and (c) washing the obtained product. The present disclosure also provides a hematology control mixture containing the nucleated red blood cell simulating particles. In addition, the present disclosure describes the use of the nucleated red blood cell simulating particles and the hematology control mixtures comprising the same, for the quality control of a blood cell analyzer. | 04-10-2014 |
| 20140106341 | STABILIZED BIOACTIVE PEPTIDES AND METHODS OF IDENTIFICATION, SYNTHESIS, AND USE - An intracellular selection system allows screening for peptide bioactivity and stability. Randomized recombinant peptides are screened for bioactivity in a tightly regulated expression system, preferably derived from the wild-type lac operon. Bioactive peptides thus identified are inherently protease- and peptidase-resistant. Also provided are bioactive peptides stabilized by a stabilizing group at the N-terminus, the C-terminus, or both. The stabilizing group can be a small stable protein, such as the Rop protein, glutathione sulfotransferase, thioredoxin, maltose binding protein, or glutathione reductase, an α-helical moiety, or one or more proline residues. | 04-17-2014 |
| 20140106342 | METHOD OF ASSAYING DNA TOPOISOMERASES AND DNA BINDING PROTEINS USING HIGH THROUGHPUT SCREENING - A novel high throughput screening (HTS) technique to rapidly identify eukaryotic topoisomerase I active agents is presented. The method is based on genetic tagging of the topoisomerase I enzyme to directly immobilize the enzyme on a solid surface in a microtiter well format. For HTS operations, DNA is added to the wells and a fraction of the input plasmid is retained on the enzyme attached to the solid phase substratum. The retained DNA is detected by Picogreen fluorescence. Compounds that result in an increase in Picogreen staining represent potential topoisomerase interfacial poisons while those that reduce fluorescence report the presence of a catalytic inhibitor; therefore, the solid phase assay represents a ‘bimodal’ readout that reveals mechanisms of action. In addition to specific topoisomerase targeting drugs, the method also weakly detects other relevant anticancer agents, such as potent DNA alkylating and intercalating compounds; therefore, topoisomerase I HTS represents an excellent tool for searching and identifying novel genotoxic agents. This solid phase HTS is rapid, robust, economical and scalable for large library screens. | 04-17-2014 |
| 20140106343 | Protocols For Making Hepatocytes From Embryonic Stem Cells - This disclosure provides a newly developed strategy and particular options for differentiating pluripotent stem cells into cells of the hepatocyte lineage. Many of the protocols are based on a strategy in which the cells are first differentiated into early germ layer cells, then into hepatocyte precursors, and then into mature cells. The cells obtained have morphological features and phenotypic markers characteristic of human adult hepatocytes. They also show evidence of cytochrome p450 enzyme activity, validating their utility for commercial applications such as drug screening, or use in the manufacture of medicaments and medical devices for clinical therapy. | 04-17-2014 |
| 20140106344 | METHODS OF TREATING A MEIOTIC KINESIN ASSOCIATED DISEASE - The invention provides methods of treating a meiotic kinase-associated disease, preferably the meiotic kinase HSET, by administering an inhibitor of the meiotic kinase. Preferably, the disease is associated with the presence of supernumerary centrosomes, such as cancer. Methods of inhibiting the growth of a tumor cell by contacting the cell with an inhibitor of a meiotic kinase, preferably HSET, are also provided. Screening methods for identifying inhibitors of the meiotic kinase HSET are also provided. Methods of selecting subjects for treatment with an inhibitor of a meiotic kinase, such as HSET, are also provided. | 04-17-2014 |
| 20140106345 | BIOMARKERS FOR IN VITRO PROGNOSIS AND DIAGNOSIS OF GRAFT AND TRASPLANT REJECTION - Novel peptides, their derivatives and compositions including the same for their use as a tool in prognosis or diagnosis of a grafted organ distress, notably of graft or transplant rejection. | 04-17-2014 |
| 20140113281 | Methods, Systems, and Computer Readable Media for Repeat Sequencing - A method for sequencing a nucleic acid template includes: (a) performing a first sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a first predetermined ordering of nucleotides and/or reagents to obtain a first sequencing result; (b) after the first sequencing process, performing a second sequencing process including flowing nucleotides and/or reagents to the nucleic acid template according to a second predetermined ordering of nucleotides and/or reagents to obtain a second sequencing result, the second predetermined ordering of nucleotides and/or reagents being different from the first predetermined ordering of nucleotides and/or reagents and at least one of the first and second predetermined orderings of nucleotides and/or reagents being designed for repeat sequencing; and (c) determining a sequence of bases corresponding to at least a portion of the nucleic acid template using both the first sequencing result and the second sequencing result. | 04-24-2014 |
| 20140113282 | LABELLED NUCLEOTIDES - Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group. | 04-24-2014 |
| 20140113283 | HIGH-SPEED SCREENING APPARATUS FOR A RAMAN ANALYSIS-BASED HIGH-SPEED MULTIPLE DRUG - The present invention relates to a high-speed screening apparatus for a Raman analysis-based high-speed multiple drug. The screening apparatus according to the present invention may easily detect a Raman signal using a core-cap-shell nanoparticle which amplifies the Raman signal by 10 | 04-24-2014 |
| 20140120527 | Nuclear localization of Src-family tyrosine kinases is required for growth factor-induced euchromatinization - A method for quantitatively evaluating chromatin structural changes using pixel imaging of the nucleus is provided. Pixel imaging of the nucleus can include capturing one or more images of a nucleus of one or more nucleic acid stain treated cells. The stain intensity can be measured by quantitating the intensity. The mean and/or standard deviation of stain intensity per pixel can be used to determine chromatin condensation levels or chromatin structural change. | 05-01-2014 |
| 20140120528 | Methods and biomarker for evaluating cancer metastasis, pharmaceutical composition for inhibiting cancer metastasis, and method for analyzing secretome - The invention relates to methods and biomarker for evaluating cancer metastasis, pharmaceutical composition for inhibiting cancer metastasis, and method for analyzing secretome. By combining a hollow fiber cartridge (HFC) culture system with quantitative proteomics technology, cancer metastasis-related secrectomes can be found. Furthermore, this is the first time to use PARK7 as a biomarker for judging the process of non-small cell lung cancer. | 05-01-2014 |
| 20140120529 | COMPOSITIONS, METHODS, SYSTEMS AND KITS FOR TARGET NUCLEIC ACID ENRICHMENT - The present invention provides methods, compositions, kits, systems and apparatus that are useful for isolating nucleic acid molecules from a sample. In particular, the methods generally relate to normalizing the concentration of target nucleic acid molecules from a sample. In one aspect, the invention relates to purifying a primer extension product from a primer extension reaction mixture. In some aspects, nucleic acid molecules obtained using the disclosed methods, kits, systems and apparatuses can be used in various downstream processes including nucleic acid sequencing. | 05-01-2014 |
| 20140120530 | METHOD FOR CLASSIFYING/COUNTING LEUKOCYTES, REAGENT KIT FOR CLASSIFYING LEUKOCYTES, AND REAGENT FOR CLASSIFYING LEUKOCYTES - The present invention provides a method for classifying and counting leukocytes which allows classification and count of normal leukocytes as well as discrimination between blast cells and atypical lymphocytes. The present invention also provides a regent kit and reagent for classifying leukocyte which are used for classifying and counting leukocytes in biological samples. | 05-01-2014 |
| 20140120531 | Devices, Solutions and Methods for Sample Collection - The disclosure relates to devices, solutions and methods for collecting and processing samples of bodily fluids containing cells (as well as embodiments for the collection, and processing and/or analysis of other fluids including toxic and/or hazardous substances/fluids). In addition, the disclosure relates generally to function genomic studies and to the isolation and preservation of cells from saliva and other bodily fluids (e.g., urine), for cellular analysis. With respect to devices for collection of bodily fluids, some embodiments include two mating bodies, a cap and a tube (for example), where, in some embodiments, the cap includes a closed interior space for holding a sample preservative solution and mates with the tube to constitute the (closed) sample collection device. Upon mating, the preservation solution flows into the closed interior space to preserve cells in the bodily fluid. The tube is configured to receive a donor sample of bodily fluid (e.g., saliva, urine), which can then be subjected to processing to extract a plurality of cells. The plurality of cells can be further processed to isolate one and/or another cell type therefrom. The plurality of cells, as well as the isolated cell type(s), can be analyzed for functional genomic and epigenetic studies, as well as biomarker discovery. | 05-01-2014 |
| 20140127677 | Compositions and Methods for the Transfer of a Hexosamine to a Modified Nucleotide in a Nucleic Acid - Nucleic acids comprising β-glucosaminyloxy-5-methylcytosine; compositions, kits and methods of producing the nucleic acids using a glycosyltransferase; and methods of using the nucleic acids are described. | 05-08-2014 |
| 20140127678 | Mapping Cytosine Modifications - Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided. | 05-08-2014 |
| 20140127679 | Chemical probe compounds that become fluorescent upon reduction, and methods for their use - Chemical stain compounds containing a fluorophore and a reducible quenching unit are disclosed. The reducible quenching unit quenches the fluorophore while in its oxidized state. Upon reduction, the quenching properties of the quenching unit are diminished or eliminated. The chemical compounds can be used in a variety of applications, including the detection of bacterial cells, monitoring the electron transport chain function of bacterial cells, monitoring the oxidation state of non-biological systems, and assaying the effectiveness of antibacterial or antimicrobial agents. | 05-08-2014 |
| 20140127680 | SINGLE MOLECULE SEQUENCING WITH TWO DISTINCT CHEMISTRY STEPS - Methods, Compositions, and Systems are provided for nucleic acid sequencing where the sequential incorporation of nucleotides uses two distinct chemical steps. A plurality of nucleotide analogs, each having a labeled leaving group at its 3′ hydroxyl can be sequentially added to a growing strand in the presence of a selective cleaving activity that cleaves the 3′ hydroxyl leaving group preferentially after it has been incorporated. The selective cleaving agent can comprise an exonuclease activity, and the exonuclease activity can be a polymerase-associated exonuclease activity. Nucleotide analogs having labels on both a cleavable polyphosphate portion and on a 3′ hydroxyl leaving group can provide signals characteristic of nucleotide analog incorporation. Systems having illumination optics, collection optics, and substrates observe signals from the labels as they are being incorporated into a growing nucleic acid strand, allowing for the sequencing of template nucleic acids. | 05-08-2014 |
| 20140127681 | SIGNAL AMPLIFICATION METHODS FOR THE IMAGING OF PROTEIN SYNTHESIS AND NEUROTRANSMITTER TRANSPORT - The present invention describes the synthesis of biological samples that can be used for the purpose of enhancing the signal-to-noise ratios achievable during the imaging of protein synthesis, amino acid transport and neurotransmitter transport, particularly in applications where single-molecule resolution is demanded. The present invention provides quencher-labeled elongation factor (EF-Tu) and fluorophore-labeled tRNA. When these molecules are present in a ternary complex with GTP, the fluorescently-labeled tRNA is quantitatively quenched. Once the tRNA is incorporated into an actively translating ribosome, however, a burst of fluorescence is released and can be detected by a variety of techniques, including smFRET imaging. The invention further provides novel EF-Tu constructs for achieving quencher labeling at high levels while retaining native or near native activity in the translation reactions, as well as methods for preparing stable ternary complexes, methods of protein sequencing, methods of detecting amino acid transport using a proteoliposome assay system and the proteoliposomes systems and methods of imaging translation events in single living cells. The present invention should have an immediate impact on next-generation sequencing technologies and the detection of neurotransmitter transporter activities in both in vitro and in vivo settings, a critical component of drug activity/screening assays targeting this important class of molecules. | 05-08-2014 |
| 20140127682 | NEW RIBOSOMAL TARGETS FOR ANTIBIOTIC DRUG DISCOVERY - The present invention relates to methods to identify molecules that binds in the neomycin binding pocket of a bacterial ribosome using structures of an intact bacterial ribosome that reveal how the ribosome binds tRNA in two functionally distinct states, determined by x-ray crystallography. One state positions tRNA in the peptidyl-tRNA binding site. The second, a fully rotated state, is stabilized by ribosome recycling factor (RRF) and binds tRNA in a highly bent conformation in a hybrid peptidyl/exit (P/E) site. Additionally, the invention relates to various assays, including single-molecule assay for ribosome recycling, and methods to identify compounds that interfere with ribosomal function by detecting newly identified intermediate FRET states using known and novel FRET pairs on the ribosome. The invention also provides vectors and compositions with an N-terminally tagged S13 protein | 05-08-2014 |
| 20140134609 | ENZYMATIC METAL NANOPARTICLE SENSOR FOR DETECTING DNA BINDERS - The present invention provides a colorimetric method for detecting a polynucleotide strand binding molecule using one type of metal particles modified with a single type of interacting molecules. The interacting molecule is capable of specifically binding to nucleic and of protecting the metal particle from aggregation. Furthermore, the metal particles are capable of aggregation upon salt aggregation and/or cleavage of the interacting molecule, and colorimetric change changes upon aggregation. | 05-15-2014 |
| 20140134610 | COMPOSITIONS AND METHODS FOR SELECTION OF NUCLEIC ACIDS - Methods are provided for reducing the complexity of a population of nucleic acids prior to performing an analysis of the nucleic acids, e.g., sequence analysis. The methods result in a subset of the initial population enriched for a target region, which is typically located within one or more target fragments. The methods are particularly useful for analyzing populations having a high degree of complexity, e.g., chromosomal-derived DNA, whole genomic DNA, or mRNA populations. | 05-15-2014 |
| 20140141413 | SYSTEM AND METHOD FOR PROCESSING PARAFFIN EMBEDDED SAMPLES - Method and apparatus for processing paraffin embedded samples, e.g., to disassociate paraffin from tissue components and/or other biomolecules from the paraffin. The sample may be exposed to focused acoustic energy while held in a vessel containing a non-solvent, aqueous solution. Disassociated paraffin may be emulsified into the liquid or otherwise separated from the sample. | 05-22-2014 |
| 20140141414 | LABELLED NUCLEOTIDES - The invention provides a nucleotide or nucleoside having a base attached to a detectable label via a cleavable linker, characterised in that the cleavable linker contains a moiety selected from the group comprising: Formula ( | 05-22-2014 |
| 20140141415 | NUCLEIC ACID ENCODING A SELF-ASSEMBLING SPLIT-FLUORESCENT PROTEIN SYSTEM - The invention provides a protein labeling and detection system based on self-complementing fragments of fluorescent and chromophoric proteins. The system of the invention is exemplified with various combinations of self-complementing fragments derived from | 05-22-2014 |
| 20140141416 | METHOD FOR HIGHLY SENSITIVE DETECTION OF PROTEIN-PROTEIN INTERACTION - The present invention intends to provide an assay system using split luciferase that has a remarkably high detection sensitivity. In an embodiment, binding of mutually binding first and second proteins is detected by preparing a first fusion protein comprising the first protein fused with a peptide having the amino acid sequence of amino acid SEQ ID NO: 1 and a second fusion protein comprising the second protein fused with a peptide having an amino acid sequence selected from the group consisting of amino acid SEQ ID NOS: 2 to 6, and allowing the first fusion protein to bind with the second fusion protein to form a complex, and detecting luminescence emitted from the complex. | 05-22-2014 |
| 20140147836 | METHODS AND APPARATUS FOR IMAGING MOLECULES IN LIVING SYSTEMS - Methods and apparatus are disclosed for imaging molecular interactions in living cells at high resolution, low light levels and high acquisition speeds. | 05-29-2014 |
| 20140147837 | METHOD FOR ANALYZING BLOOD CELLS AND BLOOD CELL ANALYZER - A method for analyzing blood cells includes: preparing a measurement specimen for classifying white blood cells, by mixing a sample with a reagent in order to hemolyze red blood cells contained in the sample and to fluorescently stain nucleic acid in white blood cells contained in the sample; detecting, by flowing the prepared measurement specimen in a flow cell, fluorescence emitted from each blood cell in the measurement specimen and two types of scattered light at respective different angles, and obtaining a fluorescence signal and two types of scattered light signals; and classifying the white blood cells into at least four groups and detecting neoplastic lymphocytes, by performing analysis using at least three types of parameters based on the obtained fluorescence signal and two types of scattered light signals. | 05-29-2014 |
| 20140154672 | Methods for Identifying Stem Cells Based on Nuclear Morphotypes - Methods for identifying stem cells and other cells specific to embryogenesis and carcinogenesis, classifying tissue samples, diagnosing precancerous and cancerous or atherosclerotic lesions, testing the value of anticancer agents, discovering macromolecules specifically expressed in particular cell types, using stem cells in restorative tissue therapy as well as methods for preparing tissue samples so heteromorphic nuclear morphotypes remain intact are disclosed. | 06-05-2014 |
| 20140154673 | PROBES OF RNA STRUCTURE AND METHODS FOR USING THE SAME - Methods for obtaining structural data from RNA in a sample, and RNA probes for performing the same, are provided. Methods of reversibly modifying RNA is a sample, in vitro or in vivo, and reversible probes for performing the same, are provided. The RNA probes may be SHAPE probes that include aryl or heteroaryl acyl imidazoles. The RNA probes may be reversible probes that include an aryl or heteroaryl ring substituted with a hydroxyl-reactive group and an azido-containing group. Also provided are methods of comparing in vitro and in vivo RNA structural data. Also provided are methods of diagnosing a cellular proliferative disease condition, e.g., by probing HOTAIR RNA. Aspects of the invention further include compositions, e.g., probes and kits, etc., that find use in methods of the invention. | 06-05-2014 |
| 20140154674 | METHODS AND KITS FOR ISOLATION AND ANALYSIS OF A CHROMATIN REGION - The present invention encompasses methods of identifying proteins and protein modifications of proteins specifically associated with a chromatin. | 06-05-2014 |
| 20140154675 | Flash and Glow 1,2-Dioxetanes - Compounds having chemiluminescent flash and glow properties. Also disclosed are methods using the compounds to generate light, detect and/or quantify enzymes, antigens, and/or nucleic acids. Also disclosed are kits relating to these compounds. | 06-05-2014 |
| 20140154676 | High Fidelity Restriction Endonucleases - Methods and compositions are provided for engineering mutant enzymes with reduced star activity where the mutant enzymes have a fidelity index (FI) in a specified buffer that is greater than the FI of the non-mutated enzyme in the same buffer. | 06-05-2014 |
| 20140154677 | CELL ANALYSIS APPARATUS AND CELL ANALYSIS METHOD - A cell analysis apparatus that can accurately distinguish between an aggregating cell and a non-aggregating cell is provided. The cell analysis apparatus ( | 06-05-2014 |
| 20140162247 | MOLECULAR TRANSISTOR - Fluidic nanotube devices and methods for their use are provided wherein the flow of charged molecules through a channel is controlled by the voltage potential of a gate electrode. In at least some embodiments, a molecular transistor is provided that includes a channel having a diameter such that only one target molecule at a time may traverse the channel. The channel may be a carbon nanotube that is electrically isolated from, and in communication with, a gate electrode. Methods are provided for controlling the flow of an individual molecule through the channel and for detecting a single chemical reaction. | 06-12-2014 |
| 20140162248 | Use of Aptamers in Liquid Chromatography and Liquid Chromatography - Mass Spectrometry - The present disclosure relates to the use of aptamers in solid phase extraction, chromatography and chromatography-mass spectrometry systems. More specifically, the present disclosure relates to the use of aptamers in SPE and chromatography systems to selectively retain, extract and/or pre-concentrate target molecule(s) having a specific affinity for the particular aptamer(s). The target molecule(s) can be further analyzed by mass spectrometry with limited interferences and/or enhanced sensitivity. | 06-12-2014 |
| 20140170643 | PARP Substrates and Biomarkers - PARylated proteins are enriched by treating cell lysates comprising PARylated proteins and DNA/RNA with an endonuclease that cleaves the DNA/RNA but not the PAR; and separating the PARylated proteins from the cleaved DNA/RNA. PARylation sites are labeled by eluting PARylated proteins from a PAR-affinity substrate with a nucleophilic amine exchange reactant, wherein the reactant labels PARylation sites of the proteins. Specific binding agents are identified by screening compounds for specific binding to a PARylated protein disclosed herein; and identifying one of the compounds as a specific binder of the protein. Antibodies which specifically bind PARylation sites are also disclosed. | 06-19-2014 |
| 20140170644 | STERILE SAMPLE INJECTOR AND METHOD - A sterile injector comprises a body having a cavity, a hollow needle positionable at a distal end of the injector, a probe holder receivable within the needle, a probe connected to a distal end of the holder, and a driving element axially displaceable within the cavity, for causing relative motion between the probe and the needle upon displacement of the driving element. A sample is injected into a sealed container by displacing the driving element to a first position causing the probe to extend from the needle, applying the extended probe with a sample, displacing the driving element to a second position causing the sample laden probe to be retracted within the needle, piercing a seal of a sealed container with the needle, and displacing the driving element to a third position distally spaced from the first position, after which the probe is injected into the container interior. | 06-19-2014 |
| 20140178862 | PHOTOINDUCED REDOX CURRENT (PIRC) DETECTION FOR DNA SEQUENCING USING INTEGRATED TRANSDUCER ARRAY - Photoinduced redox current (PIRC) detection for DNA sequencing using integrated transducer arrays is described. For example, a method of determining DNA sequencing using photoinduced redox current (PIRC) includes providing a template having a primer region and a second region of one or more unknown bases. The template is coupled to a transducer array. The method also includes incorporating a tagged nucleotide at the second region of the template. The method also includes exposing the template to a light source. The method also includes electrically detecting, by the transducer, a state of the template. The method also includes removing a photosensitizer tag of the tagged nucleotide. | 06-26-2014 |
| 20140178863 | SUBSTRATES, SYSTEMS AND METHODS FOR ANALYZING MATERIALS - Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses. | 06-26-2014 |
| 20140178864 | MODULAR NUCLEIC ACID-BASED CIRCUITS FOR COUNTERS, BINARY OPERATIONS, MEMORY, AND LOGIC - We have created novel engineered genetic counter designs and methods of use thereof that utilize DNA recombinases to provide modular systems, termed single invertase memory modules (SIMMs), for encoding memory in cells and cellular systems. Our designs are easily extended to compute to high numbers, by utilizing the >100 known recombinases to create subsequent modules. Flexibility in our engineered genetic counter designs is provided by daisy-chaining individual modular components, i.e., SIMMs together. These modular components of the engineered genetic counters can be combined in other network topologies to create circuits that perform, amongst other things, logic and memory. Our novel engineered genetic counter designs allow for the maintenance of memory and provide the ability to count between discrete states by expressing the recombinases between their cognate recognition sites. | 06-26-2014 |
| 20140178865 | RAPID, MASSIVELY PARALLEL SINGLE-CELL DRUG RESPONSE MEASUREMENTS VIA LIVE CELL INTERFEROMETRY - A central question in cancer therapy is how individual cells within a population of tumor cells respond to drugs designed to arrest their growth. However, the absolute growth of cells, their change in physical mass, whether cancerous or physiologic, is difficult to measure directly with traditional techniques. Embodiments of the invention provide live cell interferometry (LCI) for rapid, realtime quantification of cell mass in cells exposed to a changing environment. Overall, LCI provides a conceptual advance for assessing cell populations to identify, monitor, and measure single cell responses, such as to therapeutic drugs. | 06-26-2014 |
| 20140186823 | MUTANT PORES - The invention relates to mutant forms of Msp. The invention also relates to nucleic acid characterisation using Msp. | 07-03-2014 |
| 20140186824 | DYNAMIC BIOCHEMICAL TISSUE ANALYSIS ASSAYS AND COMPOSITIONS - Described herein is a method, and compositions useful with the method, of analyzing a tissue from a subject. The method includes contacting a probe conjugate ( | 07-03-2014 |
| 20140186825 | LABELED NUCLEOTIDE ANALOGS - Labeled reactant compositions, and particularly labeled nucleic acid reaction compositions that include structural components including double-stranded nucleic acids. In some embodiments, the structural components maintain potentially damaging labeling components sufficiently distal from the reactant portion of the molecule such that damaging effects of the label group on other reaction components, such as enzymes, are reduced, minimized and/or eliminated. | 07-03-2014 |
| 20140193807 | BEAD MANIPULATION TECHNIQUES - The invention provides a method of redistributing magnetically responsive beads in a droplet. The method may include providing a droplet including magnetically responsive beads. The droplet may be provided within a region of a magnetic field having sufficient strength to attract the magnetically responsive beads to an edge of the droplet or towards an edge of the droplet, or otherwise regionalize or aggregate beads within the droplet. The method may also include conducting on a droplet operations surface one or more droplet operations using the droplet without removing the magnetically responsive beads from the region of the magnetic field. The droplet operations may in some cases be electrode-mediated. The droplet operations may redistribute and/or circulate the magnetically responsive beads within the droplet. In some cases, the droplet may include a sample droplet may include a target analyte. The redistributing of the magnetically responsive beads may cause target analyte to bind to the magnetically responsive beads. In some cases, the droplet may include unbound substances in a wash buffer. The redistributing of the magnetically responsive beads causes unbound substances to be freed from interstices of an aggregated set or subset of the magnetically responsive beads. | 07-10-2014 |
| 20140199689 | METHOD FOR ISOLATING NUCLEIC ACIDS - The present invention pertains to a method for isolating nucleic acids from a sample, preferably a blood sample, comprising the following steps: a) obtaining a sample which has been stabilised by the use of at least one cationic detergent, wherein the cationic detergent has formed complexes with the nucleic acids; b) obtaining the complexes optionally together with other sample components from the stabilised sample, wherein said complexes comprise the nucleic acids to be isolated; c) resuspending the complexes and optionally adding one or more additives before, during and/or after resuspension, thereby obtaining a resuspended sample comprising at least: i) the nucleic acid to be isolated; ii) at least one chaotropic agent; and iii) at least one chelating agent; and d) isolating nucleic acids from the resuspended sample. It was found that adding a chelating agent during resuspension considerably increases the nucleic acid yield as the formation of precipitates which irreversibly adhere to the container wall is considerably reduced. | 07-17-2014 |
| 20140212868 | Method and Kit For The Isolation Of Genomic DNA, RNA Proteins and Metabolites From A Single Biological Sample - The invention provides a method and kit for the separation and purification of cellular components including polar and non-polar metabolites, genomic DNA, RNA and proteins from a single biological sample where two steps of lysis of the cells are performed sequentially, before and after a metabolite isolation step. The first lysis step is mechanical and performed in order to be incomplete, whereas the second is chemical or both mechanical and chemical. A sequential isolation of genomic DNA, RNA and proteins is carried out after the second lysis step. | 07-31-2014 |
| 20140220556 | Method of Design and Synthesis of a New Drug - A method of design and synthesis of a new drug. This invention may be used in human and veterinary medicine for the design of new drugs that are effective in the treatment of oncological and viral human and animal illnesses and for the design of new medicines. In the method a biopolymer target for the drug action is selected; then the quantity of nitrogen-containing positively charged groups available for modification is calculated. Biopolymer target may be cut into oligomer fragments. Some of calculated nitrogen-containing positively charged groups are substituted with negatively charged groups by combinatorial modification. The obtained supramolecular assemblies are used as drug for the biopolymer target. | 08-07-2014 |
| 20140220557 | Device and Method for Laser Analysis and Separation (LAS) of Particles - A device and method for particle separation. The device includes at least one collimated light source operable to generate at least one collimated light source beam. The device further includes a first channel in a first plane and a focused particle stream nozzle operably connected to the first channel. The device further includes a second channel in a second plane orthogonal to the first plane. The second channel communicates with the first channel. The second channel comprises a second channel cross-section. The second channel is oriented to receive the collimated light source beam. The device further includes a third channel in a third plane orthogonal to the second plane. The third channel communicates with the second channel. The collimated light source beam is oriented to enter a cross-section of the first channel, then to pass through the second channel, and then to enter a cross-section of the third channel. | 08-07-2014 |
| 20140220558 | Methods and Systems for Nucleic Acid Sequence Analysis - Disclosed are new and improved methods and systems for nucleic acid sequence analysis that can analyze data indicative of natural by-products of nucleotide incorporation events without the need for exogenous labels or dyes to identify nucleic acid sequences of interest. In particular, the methods and systems of the present teachings can process such data and various forms thereof to align fragments of the nucleic acid(s) of interest, particularly those analyzed using an addition sequencing technique, for example, as occurs with the use of nucleotide flows. | 08-07-2014 |
| 20140220559 | METHOD TO DETERMINE DNA MISMATCH REPAIR FUNCTION - This invention relates to a quantitative method for determining whether a human subject has an impaired DNA mismatch repair function; providing a diagnostic sample taken from said human and producing a nuclear extract from said sample; providing MMR proficient and MMR deficient nuclear extracts as positive and negative controls, respectively; combining each nuclear extract with at least one mismatch bearing substrate DNA molecule; performing a mismatch repair assay; and determining whether said sample nuclear extract is capable of repairing said substrate DNA molecule; wherein said sample comprises normal, non-malignant constitutive cells, such as fibroblasts. The invention further relates to a kit providing necessary reagents for use in said method. | 08-07-2014 |
| 20140220560 | METHODS FOR RNA DETECTION AND QUANTIFICATION - The present invention relates to novel nucleic acid molecules, called aptamers, that bind specifically to a small molecule fluorophore and thereby enhance the fluorescence signal of the fluorophore upon exposure to radiation of suitable wavelength. Molecular complexes formed between the novel fluorophores, novel nucleic acid molecules, and their target molecules are described, and the use of multivalent aptamer constructs as fluorescent sensors for target molecules of interest are also described. | 08-07-2014 |
| 20140234832 | Methods And Compositions For Inhibiting Undesired Cleaving Of Labels - The invention provides methods and compositions, including, without limitation, algorithms, computer readable Media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses. | 08-21-2014 |
| 20140242574 | CODON-OPTIMIZED GENE FOR MUTATED SHRIMP LUCIFERASE AND METHOD FOR USE THEREOF - There has been a demand for a codon-optimized gene for the mutated catalytic domain of | 08-28-2014 |
| 20140242575 | KITS AND METHODS FOR IN VITRO ANALYTE DETECTION USING PRECIPITATING COMPOUND AND POLARIZERS - The invention provides inexpensive kits and methods for determination of an analyte in a sample with very high sensitivity. Analyte can be determined using an analyte binding member such as an antibody or an oligonucleotide, which can be directly or indirectly linked to an enzyme. The kit and method uses a compound that is altered in the presence of the enzyme, and that precipitates into crystalline particulates. The reaction composition with crystalline particulates is placed between two polarizers which are in an orthogonal arrangement, and the presence of particulates is observed, correlating to the analyte in the sample. | 08-28-2014 |
| 20140242576 | METHODS FOR DETERMINING NUCLEOTIDE SEQUENCE REPEATS - The invention relates to methods to reduce the length of a homonucleotide repeat sequence a target DNA sequence. This is done by using an oligonucleotide primer which extends in the unaltered repeat of identical nucleotides of the target polynucleotide, the oligonucleotide primer comprises at the at the 5′ side a sequence which hybridizes with the sequence preceding the targeted sequence repeat, but the oligonucleotide primer is not 100% complementary with the sequence repeat in the target sequence. | 08-28-2014 |
| 20140248608 | METHODS FOR CHARACTERIZING A DEVICE COMPONENT BASED ON A CONTRAST SIGNAL TO NOISE RATIO - The general concept of using a nanopore for DNA sequencing is to electrophoretically drive a polymer (e.g. single stranded DNA) through a nanopore under aqueous conditions, and identify each individual monomer (e.g. nucleotide) of the strand as it passes through the sensitive region of the nanopore based on its characteristic current modulation. | 09-04-2014 |
| 20140255917 | POLYMETHINE COMPOUNDS AND THEIR USE AS FLUORESCENT LABELS - The present disclosure relates to new polymethine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications. | 09-11-2014 |
| 20140255918 | Compositions, Devices, Systems, and Methods for Using a Nanopore - The invention herein disclosed provides for devices and methods that can detect and control an individual polymer in a mixture is acted upon by another compound, for example, an enzyme, in a nanopore in the absence of requiring a terminating nucleotide. The devices and methods are also used to determine rapidly (˜>50 Hz) the nucleotide base sequence of a polynucleotide under feedback control or using signals generated by the interactions between the polynucleotide and the nanopore. The invention is of particular use in the fields of drug discovery, molecular biology, structural biology, cell biology, molecular switches, molecular circuits, and molecular computational devices, and the manufacture thereof. | 09-11-2014 |
| 20140255919 | MULTIBASE DELIVERY FOR LONG READS IN SEQUENCING BY SYNTHESIS PROTOCOLS - The present technology relates to molecular sciences, such as genomics. More particularly, the present technology relates to methods for obtaining long lengths of sequencing data. | 09-11-2014 |
| 20140255920 | RHODAMINE COMPOUNDS AND THEIR USE AS FLUORESCENT LABELS - The present invention relates to new rhodamine compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications. | 09-11-2014 |
| 20140255921 | ENZYME METHOD - The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a Hel308 helicase or amolecular motor which is capable of binding to the target polynucleotide at an internal nucleotide. The helicase or molecular motor controls the movement of the target polynucleotide through the pore. | 09-11-2014 |
| 20140272946 | Methods and Systems For DNA-Based Detection And Reporting - Methods, systems, and devices for the detection and notification of chemical, biological, radiological, nuclear, and/or explosive agent. The detection and notification system uses aptamers bound to a catalyst that is activated when the aptamer binds to the agent. The activated catalyst acts on a substrate to create a signal that is detected, indicating the presence of the agent. For example, the system can be a glove worn by a user that includes a catalyst/substrate pair that, in the presence of the target agent, produces a colorimetric and/or fluorescent signal in a time-efficient manner. | 09-18-2014 |
| 20140272947 | METHODS AND COMPOSITIONS FOR SCREENING MODULATORS OF ONCOGENE-INDUCED SENESCENCE - A high-throughput method for identifying a compound or biomolecule that modulates cell senescence involves simultaneously measuring in a cell population exposed to the test compound or biomolecule, the expression of a senescence marker and cell number, wherein each well contains a single test compound; and determining from said simultaneous measurements whether the test compound increases or decreases cell senescence. In various embodiments, the method is useful is identifying compounds that delay the aging process of normal healthy cells, or identifying a compound useful as a tumor suppressor or identifying a compound useful in the treatment of cancer. | 09-18-2014 |
| 20140272948 | HIERARCHICAL ASSEMBLY OF OPTICAL MAPS - The invention generally relates to optical maps and particularly to computationally tractable methods of assembling large numbers of single molecule maps by dividing the maps into smaller groups of maps within which all of the maps are similar to one another by some metric. For each group, all of the maps are assembled into contigs. The resulting contigs are then assembled into one or more genome assemblies. By dividing the maps into groups, a number of comparison operations required for assembly is reduced and, since each group of maps can be assembled into a contig in a discrete operation, the overall assembly operation can be parallelized. | 09-18-2014 |
| 20140295410 | CONJUGATE OF A METAL NANOPARTICLE AND A LIGHT EMITTING MATERIAL - Disclosed are a conjugate of a metal nanoparticle including a magnetic core and at least one light emitting material linked to the metal nanoparticle through a linker, wherein the linker has an affinity for a biological material and has changed structure after contacting a biological material, a biosensor including the conjugate, and a method of measuring a concentration of specific biological material in a biological sample using the conjugate or the biosensor. | 10-02-2014 |
| 20140295411 | METHODS AND REAGENTS FOR PRESERVING RNA IN CELL AND TISSUE SAMPLES - This specification relates to the field of molecular biology and provides novel methods and reagents for preserving and protecting the ribonucleic acid (RNA) content of samples from degradation prior to RNA isolation. This preservation may be accomplished without ultra-low temperature storage or disruption of the tissue. | 10-02-2014 |
| 20140295412 | METHODS AND SYSTEMS FOR DIRECT SEQUENCING OF SINGLE DNA MOLECULES - The invention provides improved methods for sequencing nucleic acids, e.g., for medical applications and biomedical research. The disclosed methods can be applied to rapid personalized medicine, genetic diagnosis, pathogen identification, and sequencing species genomes. | 10-02-2014 |
| 20140295413 | SYSTEMS, METHODS, AND WORKFLOWS FOR OPTOGENETICS ANALYSIS - The invention provides methods for characterizing cellular physiology by incorporating into an electrically excitable cell an optical reporter of, and an optical actuator of, electrical activity. A signal is obtained from the optical reporter in response to a stimulation of the cell. Either or both of the optical reporter and actuator may be based on genetically-encoded rhodopsins incorporated into the cell. The invention provides all optical methods that may be used instead of, or as a complement to, traditional patch clamp technologies and that can provide rapid, accurate, and flexible assays of cellular physiology. | 10-02-2014 |
| 20140295414 | METHODS AND COMPOSITIONS FOR LABELING NUCLEIC ACIDS - The present invention relates to methods for the labeling of nucleic acid polymers in vitro and in vivo. In particular, the methods include a [3+2] cycloaddition between a nucleotide analogue incorporated into a nucleic acid polymer and a reagent attached to a label. Such methods do not require fixation and denaturation and therefore can be applied to the labeling of nucleic acid polymers in living cells and in organisms. Also provided are methods for measuring cellular proliferation. In these methods, the amount of label incorporated into the DNA is measured as an indication of cellular proliferation. The methods of the invention can be used in a wide variety of applications including clinical diagnosis of diseases and disorders in which cellular proliferation is involved, toxicity assays, and as a tool for the study of chromosomes' ultrastructures. | 10-02-2014 |
| 20140295415 | LOW COST, DISPOSABLE MOLECULAR DIAGNOSTIC DEVICES - The invention provides molecular diagnostic test devices and methods for using such diagnostic test devices to detect analytes of biological significance in a patient. The diagnostic test devices are particularly useful for detecting a polynucleotide analyte in a sample obtained from a patient. Further, the diagnostic test devices are inexpensive, disposable, easy to use, and are useful at the point of care. | 10-02-2014 |
| 20140302493 | METHOD AND QUALITY CONTROL MOLECULAR BASED MOUSE EMBRYO ASSAY FOR USE WITH IN VITRO FERTILIZATION TECHNOLOGY - A method for qualitatively assessing products used in in vitro fertilization is provided. Also disclosed is an improved quality control assay for use in clinical Assisted Reproductive Technologies (ART). | 10-09-2014 |
| 20140308661 | SYSTEMS AND METHODS FOR MULTI-ANALYSIS - Systems and methods are provided for sample processing. A device may be provided, capable of receiving the sample, and performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing multiple assays. The device may comprise one or more modules that may be capable of performing one or more of a sample preparation, sample assay, and detection step. The device may be capable of performing the steps using a small volume of sample. | 10-16-2014 |
| 20140308662 | MSP NANOPORES AND RELATED METHODS - Provided herein are | 10-16-2014 |
| 20140315190 | METHODS FOR DYE SELECTION FOR PROTEIN MELT TEMPERATURE DETERMINATIONS - According to the present teachings, compositions, kits, and methods for protein melt analysis are provided that utilizing one or more fluorophore dyes. In some embodiments, a method comprises preparing a sample by mixing at least one protein with two or more dyes, and applying a controlled heating, while recording the fluorescence emission of the sample. The methods can be used, for example, for screening conditions for optimized protein stability, screening for ligands that bind and enhance protein stability (e.g., protein-protein interactions), screening for mutations for enhanced stability, screening crystallization conditions for protein stability, screening storage conditions for protein stability, and screening conditions in which a protein will be used (e.g., production conditions, treatment conditions, etc.) for protein stability. | 10-23-2014 |
| 20140315191 | FOUR-COLOR DNA SEQUENCING BY SYNTHESIS USING CLEAVABLE FLUORESCENT NUCLEOTIDE REVERSIBLE TERMINATORS - This invention provides a process for sequencing single-stranded DNA employing modified nucleotides. | 10-23-2014 |
| 20140329233 | METHODS, COMPOSITIONS AND KITS FOR A ONE-STEP DNA CLONING SYSTEM - Methods and kits for joining two or more polynucleotides to form a product polynucleotide are provided. A mixture contains a first polynucleotide comprising a selectable marker. The mixture further contains a second polynucleotide comprising a first typeIIs recognition sequence and a second typeIIs recognition sequence. The second polynucleotide is other than the first polynucleotide. The mixture further contains a first typeIIs restriction endonuclease that cleaves the first typeIIs recognition sequence to produce a first end, a second typeIIs restriction endonuclease that cleaves the second typeIIs recognition sequence to produce a second end, and a DNA ligase. The first end is not compatible with the second end. The combined actions of the enzymes in the mixture join the first polynucleotide to the second polynucleotide forming a product polynucleotide, which is obtained by transforming the mixture into a host cell. | 11-06-2014 |
| 20140329234 | FLUORESCENT LABELING OF TRANSFER RNA AND STUDY OF PROTEIN SYNTHESIS - Provided are methods for labeling transfer RNA comprising replacing the uracil component of a dihydrouridine of said transfer RNA with a fluorophore. The disclosed methods may comprise fluorescent labeling of natural tRNAs (i.e., tRNAs that have been synthesized in a cell, for example, in a bacterium, a yeast cell, or a vertebrate cell) at dihydrouridine (D) positions, or fluorescent labeling of synthetic tRNAs. In another aspect, the present invention provides methods for assessing protein synthesis in a translation system comprise providing a tRNA having a fluorophore substitution for the uracil component of a dihydrouridine in a D loop of the tRNA; introducing the labeled tRNA into the translation system; irradiating the translation system with electromagnetic radiation, thereby generating a fluorescence signal from the fluorophore; detecting the fluorescence signal; and, correlating the fluorescence signal to one or more characteristics of the protein synthesis in the translation system. The disclosed methods are useful in single molecule as well as in ensemble settings. | 11-06-2014 |
| 20140329235 | Sensor Housing and Reagent Chemistry - A sensor comprises a sensor housing, having a channel; a porous substrate, in the channel; an analysis chemistry reagent, on the porous substrate; and a nozzle, in fluid connection with the channel. The porous substrate fills a cross section of the channel, and the cross-sectional area of the channel at the porous substrate is greater than the cross-sectional area at the nozzle. | 11-06-2014 |
| 20140329236 | NON-NATURAL NUCLEOSIDES AS THERANOSTIC AGENTS - A theranostic agent for inhibiting translesion DNA replication comprises natural adenine ribose analog having formula I. | 11-06-2014 |
| 20140335509 | Method of Determining the Nucleotide Sequence of Oligonucleotides and DNA Molecules - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 11-13-2014 |
| 20140335510 | NANOPARTICLE SENSOR FOR NUCLEIC ACID-PROTEIN INTERACTION - The present invention provides a sensor for sensing nucleic acid-protein interactions, comprising a noble metal nanoparticle (NP), a double stranded nucleic acid molecule capable of binding with a protein in an aqueous solution and a fluorescent conjugated polymer (CP). The present invention also provides a method for sensing nucleic acid-protein interactions with the sensor as defined above. | 11-13-2014 |
| 20140335511 | Acoustic Pressure Wave/Shock Wave Mediated Processing of Biological Tissue, and Systems, Apparatuses, and Methods Therefor - Methods for preparing a biological tissue specimen for examination, such as microscopic examination, that involves applying acoustic pressure wave/shock wave (APW/SW) energy to the tissue specimen. The APW/SW energy can be applied to the tissue specimen to augment any one or more steps of processing the tissue specimen. In one example, the APW/SW energy is applied to enhance histotechnological processing of the tissue specimen. Apparatuses and systems that include APW/SW generators and that are specifically configured for processing biological tissue specimens are also disclosed. | 11-13-2014 |
| 20140335512 | ENZYME METHOD - The invention relates to a new method of characterising a target polynucleotide. The method uses a pore and a RecD helicase. The helicase controls the movement of the target polynucleotide through the pore. | 11-13-2014 |
| 20140342352 | HEAT-REDUCTION METHODS AND SYSTEMS RELATED TO MICROFLUIDIC DEVICES - Systems and methods for preventing or reducing unwanted heat in a microfluidic device while generating heat in selected regions of the device are described. Current can be supplied to a heating element through electric leads that are designed so that the current density in the leads is substantially lower than the current density in the heating element. Unwanted heat in the microfluidic complex can be reduced by thermally isolating the electric leads from the microfluidic complex by, for example, running each lead directly away from the microfluidic complex. Unwanted heat can be removed from selected regions of the microfluidic complex using one or more cooling devices. | 11-20-2014 |
| 20140349281 | System and Method for Dispensing Barcoded Solutions - The present disclosure provides a system capable of dispensing DNA encoding solutions at controllable flow rates and in uniform patterns, allowing sufficient DNA to be deposited onto a substrate for subsequent detection and identification of the substrate. The disclosed dispensing system may also include a feedback mechanism capable of validating that sufficient DNA has been applied to a substrate so that a barcode may be generated, detected, and identified at a later time. | 11-27-2014 |
| 20140349282 | Cell Permeable, Fluorescent Dye - The invention pertains to a near-infrared fluorescent dye that is cell permeable and can be attached to selected proteins in living cells. The dye has the general formula | 11-27-2014 |
| 20140356865 | Methods and Compositions for Efficient Base Calling in Sequencing Reactions - The present invention is directed to methods and compositions for acquiring nucleotide sequence information of target sequences. In particular, the present invention provides methods and compositions for improving the efficiency of sequencing reactions by using fewer labels to distinguish between nucleotides and by detecting nucleotides at multiple detection positions in a target sequence. | 12-04-2014 |
| 20140370498 | COMPOSITIONS AND METHODS FOR NUCLEOTIDE SEQUENCING - The invention provides nucleoside and nucleotide molecules containing cleavable linkers linking a label such as a dye. The invention also provides nucleosides and nucleotide molecules containing a blocking group, either removable or non-removable. The invention additionally provides methods of using the nucleoside and nucleotide molecules containing a cleavable linker and/or a blocking group. | 12-18-2014 |
| 20140370499 | DEVICE AND METHOD FOR FRAGMENTING POLYMERS AND PARTICLES - The invention features devices for fragmenting polymers or particles in a sample using a centrifuge. In its simplest embodiment, the device includes a body and a valve. The valve, to one side of which a sample is applied, is designed to remain closed until a specified centrifugal force is reached or exceeded. Once open, the valve allows the passage of the sample through a channel in the body. The channel includes one to four fragmenting regions, and the polymers or particles are fragmented as they traverse the fragmenting regions under an applied centrifugal force. The invention further features methods of fragmenting polymers using a device of the invention. | 12-18-2014 |
| 20140370500 | NONVIRAL TARGETED NANOPARTICLE SYSTEM FOR GENE TRANSFER AND DRUG DELIVERY - The embodiments herein provide a nanoparticle system for targeted gene delivery or a drug delivery and a method of synthesising the same. The nanoparticle composition for targeted gene transfer and drug delivery comprises a protein, a chitosan and a lipid. A method of synthesizing the nanoparticles involves preparing a gelatine and chitosan gel. A milky colloid solution is prepared with the gelatine, chitosan solution and a phosphatidylcholine. The milky colloid is homogenized for the self assembly of the nanoparticles. The milky colloid is subjected to high speed and high pressure homogenizer. The CHO cells are transfected with nanoparticles and lipofectamine 2000 for comparing the transfection efficiency. The nanoparticles deliver DNA, RNA, ribozyme and nucleotide sequences. The nanoparticles deliver lipophylic and hydrophilic drugs. The transfection efficiency of gene and drug is higher when the target cells are transferred with nanoparticles, compared to the cells transferred with lipofectamine 2000. | 12-18-2014 |
| 20140377742 | MICROFLUIDIC DEVICE - Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples. | 12-25-2014 |
| 20140377743 | CHEMICALLY CLEAVABLE 3'-O-ALLYL-DNTP-ALLYL-FLUOROPHORE FLUORESCENT NUCLEOTIDE ANALOGUES AND RELATED METHODS - This invention provides a nucleotide analogue comprising (i) a base selected from the group consisting of adenine, guanine, cytosine, thymine and uracil, (ii) a deoxyribose, (iii) an allyl moiety bound to the 3′-oxygen of the deoxyribose and (iv) a fluorophore bound to the base via an allyl linker, and methods of nucleic acid sequencing employing the nucleotide analogue. | 12-25-2014 |
| 20140377744 | NUCLEIC ACID LIGANDS AGAINST INFECTIOUS PRIONS - The invention generally relates to nucleic acid ligands that specifically bind to infectious prions, and methods of diagnosing a transmissible spongiform encephalopathy disease in a subject. In certain embodiments, the invention provides an isolated nucleic acid ligand that binds to an infectious prion. In other embodiments, the invention provides a method for diagnosing a transmissible spongiform encephalopathy disease in a subject including obtaining a tissue or body fluid sample from a subject, contacting the tissue or body fluid with a nucleic acid ligand that binds to an infectious prion, thereby detecting the infectious prion in the sample, and diagnosing the transmissible spongiform encephalopathy disease based on results of the contacting step. | 12-25-2014 |
| 20150010904 | MOLECULAR DIAGNOSTIC ASSAY DEVICE AND METHOD OF USE - Lateral flow devices and methods of use for a molecular diagnostic assay are provided. The method is suitable for detection or monitoring of targets, including biological, chemical, and material targets that exist in very low concentrations in biological samples. The methods and devices of the present application are amenable to power source-free point of care testing. | 01-08-2015 |
| 20150024384 | SEQUENTIAL DELIVERY DEVICE AND METHOD - A reagent delivery device includes a reagent delivery column with a housing receiving reagent storage elements that can move, and a breaching element coupled to the housing. The device includes an actuation member that during operation forces the reagent storage elements toward the breaching element. Breaching the storage elements releases the reagent. The breaching element and/or the housing are configured to communicate reagent between the reagent storage element and a target chamber coupled to the housing. The breaching element can be a needle, a blade, or a combination thereof. Multiple reagent delivery columns can be coupled to microplate wells for larger scale reagent delivery and processing. Biasing elements such as a spring and a counterweight can be directly or indirectly coupled to the housing and/or the reagent storage elements, resisting and/or moderating movement of the reagent elements. | 01-22-2015 |
| 20150031020 | ENZYME-PORE CONSTRUCTS - The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing. | 01-29-2015 |
| 20150037787 | POLYNUCLEOTIDE CONFIGURATION FOR RELIABLE ELECTRICAL AND OPTICAL SENSING - A mixed polynucleotide includes a first double stranded (ds) portion, a second portion including at least one single stranded (ss) portion, and a third ds portion. The second portion connects the first ds portion and the third ds portion to provide a modified polynucleotide. | 02-05-2015 |
| 20150037788 | DNA SEQUENCING BY NANOPORE USING MODIFIED NUCLEOTIDES - This invention provides a process for sequencing single-stranded DNA by employing a nanopore and modified nucleotides. | 02-05-2015 |
| 20150050641 | METHOD FOR THE ENUMERATION OF MAMMALIAN MICRONUCLEATED ERYTHROCYTE POPULATIONS, WHILE DISTINGUISHING PLATELETS AND/OR PLATELET-ASSOCIATED AGGREGATES - A method for the enumeration of micronucleated erythrocyte populations while distinguishing platelet and platelet-associated aggregates involves the use of a first fluorescent labeled antibody having binding specificity for a surface marker for reticulocytes, a second fluorescent labeled antibody having binding specificity for a surface marker for platelets, and a nucleic acid staining dye that stains DNA (micronuclei) in erythrocyte populations. Because the fluorescent emission spectra of the first and second fluorescent labeled antibodies do not substantially overlap with one another or with the emission spectra of the nucleic acid staining dye, upon excitation of the labels and dye it is possible to detect the fluorescent emission and light scatter produced by the erythrocyte populations and platelets, and count the number of cells from one or more erythrocyte populations in said sample. In particular, the use of the second antibody prevents interference by platelet-associated aggregates in the scoring procedures. | 02-19-2015 |
| 20150050642 | METHODS AND ARTICLES FOR DETECTING DEOXYRIBONUCLEASE ACTIVITY - The disclosure provides articles and methods useful for detecting a discrete source of DNase activity. DNase-producing microorganisms can be detected. The device can further include selective agents and/or indicators to differentiate groups or species microorganisms. Methods of use include detecting or enumerating DNase-producing microorganisms. | 02-19-2015 |
| 20150050643 | BLOOD ANALYZER, BLOOD ANALYSIS METHOD, HEMOLYTIC AGENT AND STAINING AGENT - This blood analyzer includes a sample preparation portion preparing a first measurement sample containing a blood sample and a hemolytic agent and a second measurement sample containing the blood sample, the same hemolytic agent as the hemolytic agent and a staining agent and a control portion classifying white blood cells in the first measurement sample into at least four groups of monocytes, neutrophils, eosinophils and others on the basis of fluorescent information and two types of scattered light information generated by a light information generation portion and classifying blood cells in the second measurement sample into at least malaria-infected red blood cells and others on the basis of fluorescent information and scattered light information generated by the light information generation portion. | 02-19-2015 |
| 20150056612 | METHODS FOR TARGETING RNA MOLECULES - Described are methods for identifying targets in RNA molecules based on differences in RNA secondary structure related to sequence variances between different allelic forms. Also described are methods for identifying or developing small molecules which can be used to modulate a target RNA by creation of protein-small-molecule-RNA complexes, as well as compositions which include such small molecules and methods of using those small molecules and compositions. | 02-26-2015 |
| 20150079584 | BIOREACTOR SYSTEM - A three dimensional cell culture and bioreactor system is provided. The system comprises one or more cell culture chamber. Each cell culture chamber comprises an inlet port and an outlet port in fluid communication with the cell culture chamber. The cell culture chambers may be segregated or in fluid communication with one another. The systems may be used to conduct drug efficacy test, isolate certain cell types from a complex tissue sample of multiple cell types, allow for the ex vivo culturing of patient tissue samples to help guide the course of treatment, and conduct co-culture experiments. | 03-19-2015 |
| 20150079585 | DNA SEQUENCING WITH REAGENT RECYCLING ON WIREGRID - The present invention relates to DNA sequencing with reagent cycling on the wiregrid. The sequencing approach suggested with which allows to use a single fluid with no washing steps. Based on strong optical confinement and of excitation light and of cleavage light, the sequencing reaction can be read-out without washing the surface. Stepwise sequencing is achieved by using nucleotides with optically cleavable blocking moietys. After read-out the built in nucleotide is deblocked by cleavage light through the same substrate. This ensures that only bound nucleotides will be unblocked. | 03-19-2015 |
| 20150079586 | System and Method for Nucleic Acids Containing Fluid Processing - A system and method for the processing of nucleic acids containing fluids involving manipulation of magnetically responsive particles contained therein are disclosed. In the system, a holder holds a plurality of containers containing the fluids, a heating device applies thermal energy to the fluids for incubation, and a separating device magnetically separates the particles. The heating and separating devices move into at least one operative position for processing the fluids and at least one inoperative position with respect to the containers, in which the containers are kept stationary at least during and in-between incubating the fluids and manipulating the magnetically responsive particles. | 03-19-2015 |
| 20150086979 | Method for Detecting Single Molecules in Living Cells and System for Use - The present invention relates in a first aspect to a method for detecting single molecules in living cells based on tracking single molecules labelled with SWNTs (Single-Walled Carbon Nanotube). In a further aspect, the present invention provides a kit comprising at least a SWNT for use in time resolved determination of single molecules in living cells as well as to a system for detecting the presence, in particular, the trajectories of single cells in living cells. | 03-26-2015 |
| 20150086980 | DEVICE AND METHOD FOR ANALYZING STREPTAVIDIN - The present invention provides a novel sensor for detecting streptavidin (SA). The nucleic acid sensor for analyzing SA of the present invention includes the following nucleic acid element that includes a catalyst nucleic acid molecule (D) that exerts a catalytic function and a binding nucleic acid molecule (A) that binds to SA. The nucleic acid element is a double-stranded nucleic acid element including a first strand and a second strand. The first strand (ss1) includes the binding nucleic acid molecule (A), a loop-forming sequence (L1), and the catalyst nucleic acid molecule (D) linked in this order. The second strand (ss2) includes a stem-forming sequence (S | 03-26-2015 |
| 20150099264 | DEVICE AND METHOD FOR PRESSURE-DRIVEN PLUG TRANSPORT AND REACTION - The present invention provides microfabricated substrates and methods of conducting reactions within these substrates. The reactions occur in plugs transported in the flow of a carrier-fluid. | 04-09-2015 |
| 20150104785 | METHODS FOR ANALYSIS OF PROTAMINE - The invention provides a method for analyzing a protamine sample for the presence or amount of at least one nucleotidic impurity. The invention also provides a method for the quantitation of peptides in a sample of protamine comprising four major peptides and at least one related impurity. | 04-16-2015 |
| 20150104786 | CELL ANALYSIS METHOD, CELL ANALYZER AND SAMPLE SCREENING METHOD - Disclosed is a cell analysis method comprising: extracting target cells from a population of cells derived from an epithelial tissue on the basis of N/C ratio representing a relative size of a nucleus to a cytoplasm; classifying the target cells into at least a first group and a second group by difference of amount of DNA; and evaluating a pathology of the epithelial tissue by comparing a ratio of numbers of cells between the first and second groups with a threshold; wherein the threshold varies according to a proportion of the target cells in the population. | 04-16-2015 |
| 20150104787 | Non-Replicative Transduction Particles and Transduction Particle-Based Reporter Systems - Methods and systems are provided for packaging reporter nucleic acid molecules into non-replicative transduction particles for use as reporter molecules. The non-replicative transduction particles can be constructed from viruses and use viral transduction and replication systems. The reporter nucleic acid molecules include a reporter gene, such as a reporter molecule or selectable marker, for detecting target genes or cells. Methods and systems are provided for detection of cells and target nucleic acid molecules using the non-replicative transduction particles as reporter molecules. | 04-16-2015 |
| 20150111202 | AUTOMATED STAINING SYSTEM AND REACTION CHAMBER - An apparatus including a reagent cartridge and a reaction chamber, the reagent cartridge having a reagent capsule removably positioned therein for dispensing of a reagent onto the reaction chamber. A system including a linearly translatable mounting assembly having a plurality of mounting stations dimensioned to receive at least one fluid dispensing cartridge, a linearly translatable bulk reagent dispensing assembly having a plurality of bulk reagent dispensing nozzles coupled thereto and a receiving assembly positioned beneath the mounting assembly and the bulk reagent dispensing assembly, the receiving assembly including a plurality of reaction stations. A method including determining an inventory of an automated sample processing system, downloading a processing protocol from a central controller to the automated sample processing system, operating the automated sample processing system based on the processing protocol and independently of the central controller and dispensing a reagent from the automated sample processing system. | 04-23-2015 |
| 20150118677 | SAMPLE PREPARATION FOR FLOW CYTOMETRY - Described herein are methods and reagents for identifying and analyzing at least one microorganism (e.g. bacteria) in a sample and reducing the background signal intensity obtained when analyzing the sample by flow cytometry. The sample is prepared by combining the sample with a background signal-reducing molecule or with a nucleic acid stain covalently linked to a quencher. A portion of the particulate matter in the sample can optionally be removed with a resin prior to staining with a nucleic acid stain. | 04-30-2015 |
| 20150118678 | Assay for Identification of Therapeutics Targeting Ternary Complex Formation in Protein Synthesis - The present invention provides a novel assay that allows high-throughput screening of chemical compounds for the inhibition of binding between EF-Tu and tRNA. | 04-30-2015 |
| 20150118679 | METHODS AND APPARATUS FOR SINGLE MOLECULE SEQUENCING USING ENERGY TRANSFER DETECTION - Provided herein are systems and methods for nucleotide incorporation reactions. The systems comprise polymerases having altered nucleotide incorporation kinetics and are linked to an energy transfer donor moiety, and nucleotide molecules linked with at least one energy transfer acceptor moiety. The donor and acceptor moieties undergo energy transfer when the polymerase and nucleotide are proximal to each other during nucleotide binding and/or nucleotide incorporation. As the donor and acceptor moieties undergo energy transfer, they generate an energy transfer signal which can be associated with nucleotide binding or incorporation. Detecting a time sequence of the generated signals, or the change in the signals, can be used to determine the order of the incorporated nucleotides, and can therefore be used to deduce the sequence of the target molecule. | 04-30-2015 |
| 20150125854 | METHODS AND COMPOSITIONS FOR PERFORMING ANALYTICAL OPERATIONS - Methods for performing analytical reactions and compositions for use in such methods, where the methods have reduced signal levels deriving from non-specific adsorption of detected reagents to other components of the analytical method, e.g., other reagents, solid phase components, vessels, etc. | 05-07-2015 |
| 20150132745 | METHODS AND COMPOSITIONS FOR GENERATING REFERENCE MAPS FOR NANOPORE-BASED POLYMER ANALYSIS - The present disclosure generally relates to the methods and compositions to efficiently analyze polymer characteristics using nanopore-based assays. Specifically disclosed is a method for generating reference signals for polymer analysis in a nanopore system, wherein the nanopore system has a multi-subunit output signal resolution. The method comprises translocating a reference sequence through a nanopore to generate a plurality of reference output signals, wherein each possible multi-subunit sequence that can determine an output signal appears only once in the reference sequence. The output signals are compiled into a reference map for nanopore analysis of an analyte polymer. Also provided are methods and compositions for calibrating the nanopore system for optimized polymer analysis. | 05-14-2015 |
| 20150132746 | SAMPLING MEDIUM - Provided herein is technology relating to the collection of biological samples and particularly, but not exclusively, to compositions, methods, and uses related to using a biopolymer substrate to collect biological samples for analysis. | 05-14-2015 |
| 20150132747 | Treatment of Neurodegeneration and Neuroinflammation - Methods of treating a subject having a condition characterized by at least one of neurodegeneration and neuroinflammation are provided. Methods of reducing astrogliosis in a subject having a condition characterized by increased astrogliosis are also provided. Methods of providing neuroprotection to a subject in need thereof are also provided. | 05-14-2015 |
| 20150132748 | INTEGRATED MICROFLUIDIC AND SOLID STATE PYROSEQUENCING SYSTEMS - The invention provides for sequencing a nucleic acid molecule based on the detection of base incorporation by the release of pyrophosphate (PPi) using a new enzyme system comprising adenosine diphosphate (ADP)-glucose pyrophosphorylase (AGPase) and its substrate ADP-glucose. | 05-14-2015 |
| 20150132749 | METHODS AND KITS FOR ISOLATING NUCLEIC ACID FROM AN ORGANISM - The invention generally relates to methods and kits for isolating nucleic acids from an organism. In certain embodiments, methods of the invention involve contacting a plurality of lytic enzymes to an organism, thereby lysing a cell wall of the organism to release the nucleic acid, and introducing at least one agent to separate the nucleic acid from the lysed cells, thereby isolating the nucleic acid. | 05-14-2015 |
| 20150140553 | HIGH EFFICIENCY MULTIPLEXED NUCLEIC ACID CAPTURE IN A STRUCTURED MICROENVIRONMENT - Provided herein is a method for sample analysis. In some embodiments, the method may involve: a) enzymatically attaching a reactive group to nucleic acid molecules in a sample; b) covalently reacting the reactive group with surface exposed reactive sites on a porous support, thereby covalently tethering the nucleic acid molecules to the porous support; c) performing a primer extension reaction using the tethered nucleic acid molecules as a template to produce primer extension products; and d) eluting the primer extension products from the porous support, while leaving the tethered nucleic acid molecules tethered to the porous support. | 05-21-2015 |
| 20150147753 | METHOD FOR PRODUCING HYPERACTIVE TRANSPOSASE MUTANTS - The present invention provides a method for producing a hyperactive MuA transposase variant comprising at least one single-amino-acid change, the method comprising the steps of modifying the nucleic acid encoding wild type MuA transposase in at least one of the positions 59, 97, 160, 179, 233, 254, 258, 302, 335, 340, 345, 374, 447, 464, 478, 482, 483, 487, 495, 507, 539, 594 or 617 so that the modified nucleic acid encodes a MuA transposase variant comprising at least one single-amino-acid change in its amino acid sequence, wherein said single-amino-acid change results in higher enzyme activity of the variant when compared to the wild type MuA transposase. The present invention also provides hyperactive MuA transposases and kits comprising the same. | 05-28-2015 |
| 20150290641 | FUNCTIONAL DEVICE AND METHOD OF MANUFACTURING THE SAME - A functional device (and a functional device manufacturing method) includes a first substrate in which a groove is formed in one surface, a second substrate which is integrally disposed by bonding one surface of the second substrate to the one surface of the first substrate, and forms a flow path together with the groove of the first substrate, at least one modification object of a capture body which captures a target substance supplied into the flow path, an electrode which imparts an electrical or a chemical action to the target substance, and a catalyst, in which the modification object is disposed by being modified on a part of an inner surface of the flow path, a bonding portion between the one surface of the first substrate and the one surface of the second substrate is formed by bonding fluorine to silica. | 10-15-2015 |
| 20150291980 | CELL CAPABLE OF PRODUCING ADENO-ASSOCIATED VIRUS VECTOR - According to the present invention, an AAV vector having a higher titer compared with those of conventional ones can be produced using a cell into which a nucleic acid capable of expressing miRNA is introduced artificially. An AAV vector produced using the cell and a composition containing the viral vector as an active ingredient are very useful as gene transfer means in the studies or clinical practice of gene therapies. | 10-15-2015 |
| 20150293023 | SYSTEMS AND METHODS FOR MEASURING TRANSLATION OF TARGET PROTEINS IN CELLS - The present invention relates to systems and methods for measuring the rate of translation of a target protein in cells, which are based on the detection of translation of one or more predetermined codon pairs during synthesis of the target protein. The detection is provided by a FRET signal emitted from labeled tRNA molecules which are juxtaposed during synthesis of the protein. | 10-15-2015 |
| 20150299714 | COMPOSITIONS AND METHODS FOR GENETIC CONSTRUCTS - In an aspect, the invention relates to compositions and methods for genetic constructs. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention. | 10-22-2015 |
| 20150300929 | CONTROLLED PRINTING OF A CELL SAMPLE FOR KARYOTYPING - Methods and techniques for controlled printing of a cell sample for karyotyping are provided. The methods can involve matrix printing using on-the-fly printing or dispensing to accurately spread cells within at least one cell sample on a surface in preparation for karyotyping, and further analysis. Advantageously, the methods result in a uniform distribution of chromosomes of the cell suspension or sample on the surface of a substrate which can be substantially discretely identified, and also provide for efficiency in a subsequent staining process and any further analysis of the stained chromosomes using a microscope or other imaging device. | 10-22-2015 |
| 20150307933 | SCAFFOLD-BASED POLYMERASE ENZYME SUBSTRATES - The invention provides a novel class of scaffold-based labeled polymerase enzyme substrates. The polymerase enzyme substrates have a multivalent core or scaffold to which is attached fluorescent dye moieties and nucleoside phosphate moities. The polymerase enzyme substrates have multiple fluorescent dye moities and/or multiple nucleoside phosphate moieties. Preferred multivalent cores comprise trifunctional six membered aromatic moities. The invention also provides for sequencing methods and kits with scaffold-based labeled polymerase enzyme substrates. | 10-29-2015 |
| 20150309041 | REACTIVE LABELLING COMPOUNDS AND USES THEREOF - Provided are azido-BODIPY compounds of formula (I), cyclooctyne-based fluorogenic probes of formula (IV), and activity-based probes of formula (VI). These compounds undergo azide alkyne cycloadditions (AAC) with to form triazolyl products. The provided compounds are useful for detection and imaging of alkyne-, or azide-containing molecules. Methods for detection and imaging biomolecules using compounds of the present disclosure are disclosed. | 10-29-2015 |
| 20150309042 | FLUOROGENIC DENDRIMER REPORTERS AND RELATED METHODS OF USE - The present invention relates to fluorogenic dendrimer reporters. In particular, the present invention relates to dendrimer nanoparticles conjugated with ‘click-on’ fluorogenic reporters and related methods of use. | 10-29-2015 |
| 20150322266 | POLYMETHINE COMPOUNDS AND THEIR USE AS FLUORESCENT LABELS - The present disclosure relates to new compounds and their use as fluorescent labels. The compounds may be used as fluorescent labels for nucleotides in nucleic acid sequencing applications. | 11-12-2015 |
| 20150322506 | Methods and Compositions for Discrimination Between Cytosine and Modifications Thereof, and for Methylome Analysis - Compositions and methods are provided for discrimination between cytosine and modifications thereof using cytidine deaminases and/or oxygenases. Variants of wild type cytidine deaminases are described which show reduced bias with respect to adjacent nucleotides upstream of the cytosine. The methods provide a rapid and convenient use of enzymes to obtain methylomes. | 11-12-2015 |
| 20150323549 | Methods for Identifying Compounds That Modulate Ion Channel Activity of a Kir Channel - Methods for identifying compounds that modulate the ion channel activity of a Kir channel are provided. Methods for identifying compounds that selectively modulate the ion channel activity of specific types of Kir channels based on the turret region of a Kir channel are also provided. Methods for identifying compounds to treat conditions associated with abnormal ion channel activity are also provided. Compounds including purified antibodies and methods of making antibodies which bind to the turret region of a Kir channel are provided. Purified polypeptides including at least a portion of the turret region of a Kir channel and nucleic acid sequences encoding these polypeptides are also provided. | 11-12-2015 |
| 20150338419 | Method for the Determination of Biomolecule Turnover Rates - Disclosed is a method for determining the turnover rate of biomolecules in a subject, which include administering to the subject, 2H20 in an amount sufficient to label biomolecules in the subject with 2H. Samples are collected from the subject at one or more time points and isotopomers are detected for the labeled biomolecules in the samples. The fractional abundance is determined for the isotopomers of the biomolecules in the samples and the biomolecule turnover rates of the one or more labeled biomolecules is determined based on the fractional abundance of the isotopomers. A computer-implemented method is also disclosed for determining the turnover rate of one or more biomolecules in subject. In certain other embodiments, a system for determining protein turnover rates in a subject is also provided. Also provided in certain embodiments is a computer program product for determining protein turnover rates in a subject. | 11-26-2015 |
| 20150355060 | METHOD AND DEVICE FOR ISOLATING CELLS FROM HETEROGENEOUS SOLUTION USING MICROFLUIDIC TRAPPING VORTICES - A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters ≧10 μm flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region. | 12-10-2015 |
| 20150355096 | DROPLET-BASED SEQUENCING METHOD - Disclosed is a method for determining the sequence of nucleotide bases in a polynucleotide analyte. It is characterised by analyte characterised by the steps of: a. generating a stream of droplets at least some of which comprise both ( | 12-10-2015 |
| 20150359911 | Aptamer Specific to Integrin alpha-v-beta-3 and Use Thereof - The present invention relates to DNA aptamer specifically binding to integrin α | 12-17-2015 |
| 20150368705 | METHOD FOR SEQUENCING A TEMPLATE NUCLEIC ACID IMMOBILIZED ON A SUBSTRATE - The present invention is directed to sequencing of nucleic acids. A method is provided for sequencing based on immobilized nucleic acid on a surface. Advantageously, a long range detection mechanism is used for detecting, whether a nucleotide provided to the substrate of a biochip has been incorporated into the immobilized template nucleic acid. Various different alignment means are provided by the present invention which can be used for facilitating a rigidly locking of the orientation of the DNA complex, which complex comprises the template nucleic acid, the primer and the capture nucleic acid. Various different linker systems may be used to immobilize the DNA complex at a first and a second strand end, such that the desired alignment of the DNA complex is achieved. Also co-adsorbed molecules on the substrate surface can be used for such an aligning measure. Additionally, or alternatively, an electrical field may be applied for repelling the DNA complex from the electrode and for facilitating a vertical DNA complex orientation. Advantageously, label-free nucleotides can be used, if desired. | 12-24-2015 |
| 20150369707 | IMPROVEMENTS IN AND RELATING TO THE TRANSFER AND STORAGE OF BIOLOGICAL MATERIAL - Apparatus for transferring biological material onto storage media, said apparatus comprising a powered hand held device including a powered hammer arrangement, the apparatus further comprising an anvil arrangement and a storage media accepting area between the hammer and the anvil, the apparatus being operable such that in use the storage media is repeatedly compressed between the hammer and the anvil by blows from the hammer. | 12-24-2015 |
| 20150369741 | URINE SPECIMEN ANALYSIS DEVICE AND URINE SPECIMEN ANALYSIS METHOD - A urine specimen analysis device includes a specimen drawing portion, a sample preparing portion, a measurement portion, and an information processing portion. The specimen drawing portion draws a first aliquot and a second aliquot from a urine specimen. The sample preparing portion prepares a first measurement sample by mixing the first aliquot and a first staining dye that stains red blood cells, and a second measurement sample by mixing the second aliquot and a second staining dye that stains nucleic acids. The measurement portion measures fluorescence emitted from the first measurement sample prepared by the sample preparing portion, and fluorescence emitted from the second measurement sample prepared by the sample preparing portion. The information processing portion detects at least red blood cells contained in the first measurement sample based on the fluorescence of the first measurement sample measured by the measurement portion, and at least white blood cells contained in the second measurement sample based on the fluorescence of the second measurement sample measured by the measurement portion. | 12-24-2015 |
| 20150376620 | VASCULAR ENDOTHELIAL GROWTH FACTOR-BINDING APTAMERS - Disclosed is providing, a novel VEGF-binding aptamer whose affinity to VEGF is higher than those of known VEGF-binding aptamers. By a in silico maturation method starting from a known VEGF-binding aptamer, 4 kinds of aptamers whose affinities to VEGF are higher than that of the known VEGF-binding aptamer were prepared. By linking two molecules of an obtained aptamer to each other via a linker, an aptamer having an even higher affinity to VEGF was obtained. The polynucleotide of the present invention contains the base sequence of any one of SEQ ID NOs:1 to 4, and binds to vascular endothelial growth factor. | 12-31-2015 |
| 20150376682 | HEAT-REDUCTION METHODS AND SYSTEMS RELATED TO MICROFLUIDIC DEVICES - Systems and methods for preventing or reducing unwanted heat in a microfluidic device while generating heat in selected regions of the device are described. Current can be supplied to a heating element through electric leads that are designed so that the current density in the leads is substantially lower than the current density in the heating element. Unwanted heat in the microfluidic complex can be reduced by thermally isolating the electric leads from the microfluidic complex by, for example, running each lead directly away from the microfluidic complex. Unwanted heat can be removed from selected regions of the microfluidic complex using one or more cooling devices. | 12-31-2015 |
| 20160002269 | LANTHANIDE CLUSTERS AND METHODS OF USE THEREOF - The present invention is directed to multinuclear lanthanides chiral clusters, based on phenyl-oxazoline-amide (POxA) ligands, and to methods of use thereof. The chiral clusters of this invention are highly fluorescent with high stability. | 01-07-2016 |
| 20160002708 | METHODS AND APPARATUS FOR TREATING SAMPLES WITH ACOUSTIC ENERGY - This invention relates to systems and methods for applying acoustic energy to a sample. According to one aspect of the invention, a system comprises a housing, a chamber for receiving the sample, an acoustic energy source for providing a focused acoustic field to the sample according to a treatment protocol, a processor for determining the treatment protocol, a sensor for detecting information about the sample, and a user interface for communicating with a user. | 01-07-2016 |
| 20160002721 | MODIFIED NUCLEOSIDES OR NUCLEOTIDES - Some embodiments described herein relate to modified nucleotide and nucleoside molecules with novel 3′-hydroxy protecting groups. Also provided herein are methods to prepare such modified nucleotide and nucleoside molecules and sequencing by synthesis processes using such modified nucleotide and nucleoside molecules. | 01-07-2016 |
| 20160003717 | Laser Capture Microdissection (LCM) Extraction Device and Device Carrier, and Method for Post-LCM Fluid Processing - The present invention generally discloses an extraction system that provides a locale for fluid processing and extraction on a post-microcapture transfer film. The extraction system includes a transfer film carrier and an extraction device forming a reservoir. The extraction system selectively excludes regions of the transfer film from the reservoir to advantageously reduce contamination due to matter adhered to the transfer film by non-specific transfer. | 01-07-2016 |
| 20160010147 | ENZYME STALLING METHOD | 01-14-2016 |
| 20160017341 | UNIVERSAL PROTEIN OVEREXPRESSION TAG COMPRISING RAMP FUNCTION, AND APPLICATION THEREOF - Provided is a ramp tag capable of solving instability in translation rate resulting from poor compatibility between codons in a foreign gene and a host when expressing a recombinant protein in | 01-21-2016 |
| 20160017413 | ENZYMES RESISTANT TO PHOTODAMAGE - Provided are compositions comprising modified DNA polymerases that exhibit improved photostability compared to the parental polymerases from which they were derived. Provided are methods for generating enzymes, such as DNA polymerases, with the aforementioned phenotype. Provided are methods of using polymerases with increased resistance to photodamage to make a DNA or to sequence a DNA template. | 01-21-2016 |
| 20160024492 | REMOVAL OF DNA FRAGMENTS IN MRNA PRODUCTION PROCESS - The present invention describes methods of removing DNA from an RNA transcript during the mRNA production process. The method embodies procedures for obtaining an in vitro transcription product, and removing any DNA from the product. The DNA can be removed by adding either free DNase or a resin containing immobilized DNase to the product, and recovering the RNA transcript. Alternatively, the DNA template used in the in vitro transcription reaction is labeled. After transcription, the product is applied to a resin that is configured to bind the label, and the RNA transcript is recovered. To detect whether any residual impurities are left in the RNA transcript product, the product is subjected to nuclease digestion and subsequently to liquid chromato graphy-tandem mass spectrometry analysis to quantitate any residual DNA. The present invention demonstrates efficient and effective methods of isolating an RNA transcript from an in vitro transcription product. | 01-28-2016 |
| 20160024577 | Methods for Identifying Stem Cells Based on Nuclear Morphotypes - Methods for identifying stem cells and other cells specific to embryogenesis and carcinogenesis, classifying tissue samples, diagnosing precancerous and cancerous or atherosclerotic lesions, testing the value of anticancer agents, discovering macromolecules specifically expressed in particular cell types, using stem cells in restorative tissue therapy as well as methods for preparing tissue samples so heteromorphic nuclear morphotypes remain intact are disclosed. | 01-28-2016 |
| 20160025710 | INTERNAL FOCUS REFERENCE BEADS FOR IMAGING CYTOMETRY - The invention generally relates to analytical and monitoring systems useful for analyzing and measuring cells and biological samples. More particularly, the invention provides systems and methods for internal calibration and focus reference for cytometry imaging. | 01-28-2016 |
| 20160032370 | Methods of Elongating DNA - The present invention relates to methods of elongating chromosomes. Embodiments of the present disclosure are directed to methods of elongating DNA by immobilizing or attaching the DNA to a substrate. According to one aspect, naturally occurring DNA includes a nucleic acid and one or more factors bound thereto, and may be referred to herein as “starting DNA | 02-04-2016 |
| 20160032377 | MODIFIED POLYMERASES FOR IMPROVED INCORPORATION OF NUCLEOTIDE ANALOGUES - Presented herein are polymerase enzymes for improved incorporation of nucleotide analogues, in particular nucleotides which are modified at the 3′ sugar hydroxyl, as well as methods and kits using the same. | 02-04-2016 |
| 20160032378 | MODIFIED NUCLEOTIDES - The invention provides modified nucleotide or nucleoside molecule comprising a purine or pyrimidine base and a ribose or deoxyribose sugar moiety having a removable 3′-OH blocking group covalently attached thereto, such that the 3′ carbon atom has attached a group of the structure —O—Z wherein Z is any of —C(R′)2-O—R″, —C(R′)2-N(R″)2, —C(R′)2-N(H)R″, —C(R′)2-S—R″ and —C(R′)2-F, wherein each R″ is or is part of a removable protecting group; each R′ is independently a hydrogen atom, an alkyl, substituted alkyl, arylalkyl, alkenyl, alkynyl, aryl, heteroaryl, heterocyclic, acyl, cyano, alkoxy, aryloxy, heteroaryloxy or amido group, or a detectable label attached through a linking group; or (R′)2 represents an alkylidene group of formula ═C(R′″)2 wherein each R′″ may be the same or different and is selected from the group comprising hydrogen and halogen atoms and alkyl groups; and wherein said molecule may be reacted to yield an intermediate in which each R″ is exchanged for H or, where Z is —C(R′)2-F, the F is exchanged for OH, SH or NH2, preferably OH, which intermediate dissociates under aqueous conditions to afford a molecule with a free 3′OH; with the proviso that where Z is —C(R′)2-S—R″, both R′ groups are not H. | 02-04-2016 |
| 20160033471 | Optoelectronic Control Of Solid-State Nanopores - Optoelectronic control of solid-state nanopores and applications thereof. Nanopores are extremely sensitive single-molecule sensors. Electron beams have been used to fabricate synthetic nanopores in thin solid-state membranes with sub-nanometer resolution. Methods for controlling the translocation speed of biopolymers through solid-state nanopores and methods for unblocking clogged pores by illuminating nanopores are described. | 02-04-2016 |
| 20160033479 | SYSTEMS AND METHODS FOR DETECTION OF CELLULAR STRESS - There are provided methods for detection and measurement of stress in a cell, the method including introducing a labeled tRNA into the cell and detecting a change in subcellular localization of the labeled tRNA in the cell, based on the signal emitted from the labeled tRNA. There are further provided methods and systems for the generation of a stress index of a living cell. There are further provided methods and systems for detection of stress in a living cell, comprising detection of changes in subcellular localization of labeled tRNA in a cell, wherein the detection is performed in real time. | 02-04-2016 |
| 20160033488 | Assay Enhancement by Selective Deposition and Binding on Amplification Structures - This disclosure provides, among other things, a method for enhancing detection of an analyte that is bound to a substrate comprising a signal amplification layer on a surface of the substrate, wherein the signal amplification layer comprises high-amplification regions and low-amplification regions, and the high-amplification regions amplify signals at said surface more than the low-amplification regions. The method comprises selectively masking the low-amplification regions of the substrate, thereby increasing the probability that an analyte will bind to a high-amplification region and be detected. | 02-04-2016 |
| 20160033495 | Detection of Non-Nucleic Acid Analytes Using Strand Displacement Exchange Reactions - The present invention relates to an analyte detection system for detecting analytes different from DNA and RNA. The system comprises a set of oligonucleotides which may hybridize to each other in specific ways and is able to generate a signal based on the specific hybridization events. The system relies on changes in the hybridization equilibrium between the oligonucleotides in the presence of an analyte or analytes, which results in a change in signal. | 02-04-2016 |
| 20160033520 | FLUORESCENT COMPOUNDS - The present invention relates to fluorescent dyes in general. The present invention provides a wide range of fluorescent dyes and kits containing the same, which are applicable for labeling a variety of biomolecules, cells and microorganisms. The present invention also provides various methods of using the fluorescent dyes for research and development, forensic identification, environmental studies, diagnosis, prognosis, and/or treatment of disease conditions. | 02-04-2016 |
| 20160040225 | MODIFIED NUCLEOTIDE LINKERS - Some embodiments of the present application relate to novel modified nucleotide linkers for increasing the efficiency of nucleotide incorporation in Sequencing by Synthesis applications. Methods of preparing these modified nucleotide linkers are also provided herewith. | 02-11-2016 |
| 20160045187 | DEVICES AND METHODS FOR COLLECTING AND STABILIZING BIOLOGICAL SAMPLES - The present invention generally relates to devices and methods for collecting and stabilizing biological samples, and more particularly, for collecting and stabilizing blood or other bodily fluids from a user's fingertip, earlobe, heel or other locations. The present invention also relates to sample collection devices that simplify the process for mixing the biological samples with an additive or additives, provide for efficient storage and safe transport of the samples, and provide for easy access to the samples for subsequent processing. | 02-18-2016 |
| 20160045610 | COMPOSITIONS OF MATTER THAT REDUCE PAIN, SHOCK, AND INFLAMMATION BY BLOCKING LINOLEIC ACID METABOLITES AND USES THEREOF - A method for treating and/or diagnosing pain and the source or type of pain, shock, and/or inflammatory conditions in a subject. A method of using a therapeutically effective amount of a DNA or RNA aptamer that shows high affinity for OLAMs to at least partially treat pain, shock, and/or inflammatory conditions in a subject. The DNA or RNA aptamer that shows high affinity for OLAMs may be coupled to a plasma protein binding compound or a pharmacologically active agent. A method of treating and or diagnosing pain, shock, and/or inflammatory conditions in a subject may include inactivating or preventing at least one linoleic acid metabolite to treat certain conditions (e.g., pain, shock, and/or inflammation) using a DNA or RNA aptamer that shows high affinity for OLAMs. | 02-18-2016 |
| 20160046679 | LOW MOLECULAR WEIGHT SILK COMPOSITIONS AND STABILIZING SILK COMPOSITIONS - The present disclosure provides certain silk-fibroin compositions with particular characteristics and/or properties. In some embodiments, the disclosure provides low molecular weight compositions. In some embodiments, the disclosure provides silk fibroin compositions that comprise an active (e.g., a biological) agent or component. In some embodiments, the disclosure provides low molecular weight silk fibroin compositions that comprise an active (e.g., a biological) agent or component. In some embodiments, an active agent is stabilized in a silk composition, e.g., for a period of time and/or against certain conditions or events. In some embodiments, a component present in a silk fibroin composition may be subject to analysis and/or characterization. In some embodiments, a component present in a silk fibroin composition may be recovered from the composition. | 02-18-2016 |
| 20160047826 | COMPOSITIONS AND METHODS FOR DETECTION OF A TARGET IN A MOLECULAR ASSAY USING PH CHANGES - The disclosure provides a sensor for detecting a target comprising a probe that is able to recognize the presence of the target; a pH-changing enzyme conjugated to an oligonucleotide that senses the recognition of the presence of the target by the probe; and a solid support linked or linkable to the probe; wherein the presence of the target causes the sensor to be either captured to the solid support or released into solution for detection of pH changes. Also provided are methods for using the sensor and kits. | 02-18-2016 |
| 20160053300 | NANOPORE BIOSENSORS FOR DETECTION OF PROTEINS AND NUCLEIC ACIDS - Described herein are nanopore biosensors based on a modified cytolysin protein. The nanopore biosensors accommodate macromolecules including proteins and nucleic acids, and may additionally comprise ligands with selective binding properties. | 02-25-2016 |
| 20160061821 | SAMPLE ANALYZER AND SAMPLE ANALYZING METHOD - Disclosed is a sample analyzer for analyzing a sample, including: a preparing unit that mixes a sample, a surfactant-containing diluent, and a nucleic acid staining reagent to prepare a measurement specimen in which nucleic acids of nucleated cells are stained and red blood cells are hemolyzed; a detecting unit that irradiates particles included in the measurement specimen with light to receive scattered light and fluorescence light emitted from the particles and output a detection signal; and a processing unit that counts white blood cells and fungi in the sample based on the detection signal. | 03-03-2016 |
| 20160069871 | MAGNETIC BEAD, LIGAND-BINDING BEAD, METHOD FOR DETECTING OR SEPARATING TARGET SUBSTANCE, AND METHOD FOR PRODUCING THE MAGNETIC BEAD - A magnetic bead, formed by binding a chain polymer at least to the surface, in which the chain polymer is a chain polymer which has a hydrophilic repeating unit, and has a group comprising a reactive functional group at the end of the side, to which the magnetic bead does not bind, through an imino group or an N-substituted imino group, and a density of the chain polymer occupying the surface of the magnetic bead is 0.1 polymers/nm | 03-10-2016 |
| 20160074859 | SAMPLE HOLDER FOR ANALYSIS OF SOLID BIOLOGICAL SAMPLES - A biological sample holder for holding a solid phase sample, including a handle, and a seal area suitable for being received in an opening of a sample receiving chamber of a cassette, mountable to a sample analysis instrument. The holder further includes a stem connected to the seal and a sample retainer connected to the stem for retaining solids in the retainer, the sample retainer including a perforated wall region for allowing fluids to pass through the wall but preventing the solid phase sample from passing through the wall. | 03-17-2016 |
| 20160076091 | NANOPORE-BASED NUCLEIC ACID ANALYSIS WITH MIXED FRET DETECTION - Various methods, systems and devices for optical detection and analysis of polymers, such as polynucleotides, using nanopores, e.g., for determining sequences of nucleic acids, are provided herein. In certain variations, methods and systems for determining a nucleotide sequence of a polynucleotide, which include measuring mixed FRET signals as a polynucleotide translocates through a nanopore and determining a nucleotide sequence of the polynucleotide from the mixed FRET signals, are provided. | 03-17-2016 |
| 20160076092 | ENZYME-PORE CONSTRUCTS - The invention relates to constructs comprising a transmembrane protein pore subunit and a nucleic acid handling enzyme. The pore subunit is covalently attached to the enzyme such that both the subunit and enzyme retain their activity. The constructs can be used to generate transmembrane protein pores having a nucleic acid handling enzyme attached thereto. Such pores are particularly useful for sequencing nucleic acids. The enzyme handles the nucleic acid in such a way that the pore can detect its component nucleotides by stochastic sensing. | 03-17-2016 |
| 20160077078 | REDUCING BACKGROUND FLUORESCENCE IN MEMS MATERIALS BY LOW ENERGY ION BEAM TREATMENT - Methods for fabricating materials useful for optical detection in microfluidic and nanofluidic devices, such as those used in nanopore-based nucleic acid sequencing are described herein. In certain variations, a method of reducing background fluorescence in a MEMS material may include the step of treating a surface of the MEMS material with a low energy ion beam. | 03-17-2016 |
| 20160077087 | FLUIDIC CONNECTORS AND MICROFLUIDIC SYSTEMS - Fluidic connectors, methods, and devices for performing analyses (e.g., immunoassays) in microfluidic systems are provided. In some embodiments, a fluidic connector having a fluid path is used to connect two independent channels formed in a substrate so as to allow fluid communication between the two independent channels. One or both of the independent channels may be pre-filled with reagents (e.g., antibody solutions, washing buffers and amplification reagents), which can be used to perform the analysis. These reagents may be stored in the channels of the substrate for long periods amounts of time (e.g., 1 year) prior to use. | 03-17-2016 |
| 20160077088 | MOLECULAR BIOSENSORS WITH A MODULAR DESIGN - The invention generally provides molecular biosensors with modular epitope binding constructs. The molecular biosensors are useful in several methods including in the identification and quantification of target molecules. | 03-17-2016 |
| 20160084830 | SURFACE PLASMON RESONANCE BASED SENSING DEVICES AND METHODS FOR REAL-TIME ANALYSIS OF ANALYTE SECRETION FROM LIVING CELLS - The present invention provides surface plasmon resonance (SPR) based sensing systems and methods for rapid, sensitive, and real-time analysis of analyte secretion from living cells. In one embodiment, the SPR based sensing device of the present invention comprises at least one cell culture module for culturing living cells, wherein the cell culture module is configured so that analytes secreted from the living cells can be released onto a SPR sensing surface. | 03-24-2016 |
| 20160090580 | HIV TYPE 1 GROUP O REVERSE TRANSCRIPTASES THAT ARE ACTIVE AT HIGH TEMPERATURES - The present invention falls within the field of biotechnology. More specifically, the invention relates to reverse transcriptases expressed and purified in bacteria and having the amino acid sequence of the reverse transcriptase of a human immunodeficiency virus type 1 (HIV-1) group O, modified at positions 358, 359 and 360; and variants of this enzyme that contain additional changes at positions 355 and 357 or at 478 or position 69 (in this case accompanied by an insertion of two amino acids). These polymerases have greater activity than the non-mutated enzyme at high temperatures (above 60° C.). In addition, they retain the capacity for DNA synthesis at temperatures greater than 70° C. Moreover, the copying fidelity of these enzymes is not significantly different from that of the non-mutated reverse transcriptase. | 03-31-2016 |
| 20160091410 | CELL PROCESSING USING MAGNETIC PARTICLES - The present invention relates to compositions comprising magnetic particles, the methods of using these compositions in processing animal sperm, the resulting sperm and embryo products, and the methods of use of these compositions to increase the efficiency, efficacy and/or speed of cell processing and artificial insemination techniques. | 03-31-2016 |
| 20160097707 | METHODS OF COLLECTING CELLS FROM MULTI-WELL PLATES FOR USE IN FLOW CYTOMETRY - A method of collecting cells from individual wells of a multi-well plate for use in flow cytometry, the method including adding a suspension of cells to wells of the multi-well plate; and aspirating cells from different wells according to a collection pattern into a flow cytometer, wherein the collection pattern is a sequential ordering of wells beginning at a middle region of the multi-well plate and continuing towards an outer region of the multi-well plate. The method preferably including rotating or agitating the multi-well plate between steps of aspirating cells from different wells. Exemplary collection patterns include spiral-square collection pattern and a nearest well to center collection pattern. | 04-07-2016 |
| 20160102357 | NOVEL RUTHENIUM (II) COMPLEXES, PREPARATION AND USES THEREOF - The present invention discloses novel Ruthenium (II) polypyridyl complexes, preparation and its application as DNA imaging agents. | 04-14-2016 |
| 20160115512 | THERMUS SCOTODUCTUS NUCLEIC ACID POLYMERASES - The invention provides nucleic acids and polypeptides for a nucleic acid polymerase from a thermophilic organism, | 04-28-2016 |
| 20160122832 | G-PROTEIN COUPLED RECEPTOR (GPCR)-BASED BIOSENSORS AND USES THEREOF - Provided herein are GPCR-based chemical biosensors that can have a sensing unit, a processing unit, and a response unit that can be used to detect a chemical of interest. Also provided herein are methods of making and using the GPCR-based chemical biosensors. | 05-05-2016 |
| 20160130644 | THIOLATED NUCLEOTIDE ANALOGUES FOR NUCLEIC ACID SYNTHESIS - The present disclosure provide systems, compositions, methods, reagents, kits and products for extending a nucleic acid that includes incorporating a nucleotide residue at a terminus of a nucleic acid using a polymerase enzyme and at least one nucleotide, wherein the at least one nucleotide includes a thiophosphate moiety, and wherein the at least one nucleotide is resistant to hydrolysis by phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of a phosphatase. In some embodiments, the nucleotide incorporation can be conducted in the presence of at least on chelation moiety that is configured to bind an orthophosphate moiety. | 05-12-2016 |
| 20160131640 | MICROFLUIDIC DEVICE - Described herein are particular embodiments relating to a microfluidic device that may be utilized for cell sensing, counting, and/or sorting. Particular aspects relate to a microfabricated device that is capable of differentiating single cell types from dense cell populations. One particular embodiment relates a device and methods of using the same for sensing, counting, and/or sorting leukocytes from whole, undiluted blood samples. | 05-12-2016 |
| 20160138079 | Mapping Cytosine Modifications - Methods, compositions and kits for selectively altering and detecting modified cytosine residues are provided. | 05-19-2016 |
| 20160138100 | METHOD OF DETERMINING THE NUCLEOTIDE SEQUENCE OF OLIGONUCLEOTIDES AND DNA MOLECULES - The present invention relates to a novel method for analyzing nucleic acid sequences based on real-time detection of DNA polymerase-catalyzed incorporation of each of the four nucleotide bases, supplied individually and serially in a microfluidic system, to a reaction cell containing a template system comprising a DNA fragment of unknown sequence and an oligonucleotide primer. Incorporation of a nucleotide base into the template system can be detected by any of a variety of methods including but not limited to fluorescence and chemiluminescence detection. Alternatively, microcalorimetic detection of the heat generated by the incorporation of a nucleotide into the extending template system using thermopile, thermistor and refractive index measurements can be used to detect extension reactions. | 05-19-2016 |
| 20160139009 | PARTICLE MIXING - A method of separating an analyte from a biological sample is described. The method comprises providing particles capable of binding said analyte when present in a solution in a container, said container comprising walls, wherein at least a part of said walls is flexible. The particles are suspended in the solution by exerting a force on the flexible part of the walls of the container more than one time. An aliquot of the suspended particles is then removed from the container. The removed aliquot is dispensed into a sample, and the sample is incubated under conditions suitable to immobilize said analyte on the particles. The particles with the bound analyte are then separated from other material and at least part of the biological sample is removed. | 05-19-2016 |
| 20160139015 | METHOD AND DEVICE FOR ISOLATING CELLS FROM HETEROGENEOUS SOLUTION USING MICROFLUIDIC TRAPPING VORTICES - A method of isolating cells includes providing a microfluidic device having at least one microfluidic channel coupled to an inlet and an outlet, the at least one microfluidic channel comprises at least one expansion region disposed along the length thereof. The at least one expansion region is an abrupt increase in a cross-sectional dimension of the at least one microfluidic channel configured to generate a vortex within the at least one expansion region in response to fluid flow. A solution containing a population of cells at least some of which have diameters ≧10 μm flows into the inlet. A portion of cells is trapped within vortex created within the at least one expansion region. The trapped cells may then released from the expansion region. | 05-19-2016 |
| 20160154013 | DRUG TARGET CAPTURING METHOD | 06-02-2016 |
| 20160169875 | NUCLEIC ACID SENSOR FOR MELAMINE ANALYSIS, DEVICE FOR MELAMINE ANALYSIS, AND METHOD FOR MELAMINE ANALYSIS | 06-16-2016 |
| 20160177387 | NOVEL ELECTROCHEMICAL DNA BIOSENSOR USING GRAPHENE BIOCHIP FOR SPECIES IDENTIFICATION | 06-23-2016 |
| 20160186243 | LABELED ENZYME COMPOSITIONS, METHODS AND SYSTEMS - Disclosed herein are conjugates comprising a biomolecule linked to a label that have biological activity and are useful in a wide variety of biological applications. For example, provided herein are labeled polymerase conjugates including a polymerase linked to one or more labels, wherein the conjugate has polymerase activity. Such conjugates can exhibit enhanced biological activity and/or superior detectability as compared to conventional labeled polymerases. Also disclosed herein are improved methods for preparing such conjugates, and methods and systems for using such conjugates in biological applications such as nucleotide incorporation, primer extension and single molecule sequencing. | 06-30-2016 |
| 20160193605 | Selective Delivery of Material to Cells | 07-07-2016 |
| 20160195520 | CAPACITANCE SPECTROSCOPIC METHOD AND ELECTRODE | 07-07-2016 |
| 20160195521 | Analyte Detection | 07-07-2016 |
| 20160195523 | DISCONTINUOUS FLUIDIC SYSTEMS FOR POINT-OF-CARE ANALYTE MEASUREMENT | 07-07-2016 |
| 20160201051 | SYSTEMS AND METHODS FOR QUANTIFYING AN ANALYTE EXTRACTED FROM A SAMPLE | 07-14-2016 |
| 20160252507 | ASSAYS FOR MACROMOLECULAR ANALYTES | 09-01-2016 |
| 20160376541 | PROCESS FOR SAPONIN ENHANCED AUTOLOYSIS OF YEAST - Process for enhancing production rates/production of yeast cell wall products and yeast extracts by adding saponin or a saponin containing ingredient to yeast cultures during fermentation or to yeast cream prior to autolysis. In the context of a sugar beet processing facility, saponin, which is contained within the sugar refining process streams during sucrose production, is readily available and can be introduced to the yeast cultures or yeast cream as either a saponin extract or as dried and shredded sugar beet leaves, without requiring any additional sourcing or acquisition costs. Activity between the saponin and yeast/yeast cream results in the formation of saponin fermentation products. | 12-29-2016 |
| 20160376595 | PEPTIDE APTAMERS FOR MANIPULATING PROTEIN FUNCTION - Peptide aptamers and the methods to produce cassettes including the aptamers and manipulating them, are described. The peptide aptamer cassettes are useful to, e.g., inhibit protein function such as proteins necessary for the transformation of plants, or to replicate cells. | 12-29-2016 |
| 20160377606 | ELECTROCHEMICAL APTASENSORS WITH A GELATIN B MATRIX - This invention provides: —an aptamer-based electrochemical sensor, wherein said aptamer is covalently bonded to or chemisorbed on an electrode, said aptamer forming a complex with a target molecule and is encapsulated by a gelatin B matrix; —a method of manufacturing said aptamer-based electrochemical sensor; —the use of the aptamer-based electrochemical sensor for the electrochemical determination of a concentration of a target molecule; and —a composite electrode combining a polymeric material and electrically conducting particles for selective analyte detection, wherein said electrode is coated with gelatin type B. | 12-29-2016 |
| 20160379811 | METHOD OF DETERMINING CELL CYCLE STAGE DISTRIBUTION OF CELLS - A method of determining a cell cycle stage distribution of cells includes the steps of providing a cell sample; pre-treating the cell sample with a solvent; mixing the pre-treated cell sample with a matrix solution to obtain a mixture solution; depositing the mixture solution on a sample plate; obtaining a mass spectrum analysis of the deposited mixture solution; and identifying at least two marker peaks from the mass spectrum analysis, wherein a ratio between the marker peaks provides information about a cell cycle stage distribution of the cell sample, wherein the mass spectrum analysis is a matrix-assisted laser desorption/ ionization time-of-flight mass spectrum test. | 12-29-2016 |
| 20170232437 | DEVICE FOR SAMPLE COLLECTION, TRANSPORTATION, AND PROCESSING | 08-17-2017 |
| 20170233720 | Sample Preparation Device and Methods of Use | 08-17-2017 |
| 20170234850 | Nanopore Detection of Small Molecules Through Competition Assays | 08-17-2017 |
| 20170234863 | ANALYTE SENSOR AND ANALYTE SENSING METHOD | 08-17-2017 |
| 20180021781 | NANOFLUIDIC FLOW CELL AND METHOD OF LOADING SAME | 01-25-2018 |
| 20180023139 | METHODS OF IDENTIFYING ESSENTIAL PROTEIN DOMAINS | 01-25-2018 |
| 20180024148 | BLOOD ANALYZER AND BLOOD ANALYZING METHOD | 01-25-2018 |
| 20190144873 | TRANSLATIONAL CONTROL SYSTEM USING RNA-PROTEIN INTERACTION MOTIF | 05-16-2019 |
| 20190144919 | METHODS AND DEVICES FOR STORING OR STABILIZING MOLECULES | 05-16-2019 |
| 20190145013 | Enzymatic Nucleic Acid Synthesis | 05-16-2019 |
