Entries |
Document | Title | Date |
20080214786 | Modified Powder, Fluid Composition Containing Said Modified Powder, Formed Article, and Method For Producing Modified Powder - The modifying powder is silk powder having an average particle diameter of 10 μm or less, oil absorption of 130 ml/100 g or less and particle diameter of 80 μm or less on the basis of dispersion degree evaluation with a grind gauge according to JIS K5400; or polysaccharide powder having an average particle diameter of 10 μm or less, and ink viscosity of less than 15 Pa·s or oil absorption of 85 ml/100 g or less. Such modifying powders can be obtained through a method comprising a first pulverization process for pulverizing a starting material for modifying material using a dry-type mechanical pulverizer such as a ball mill, and a second pulverization process for pulverizing the obtained pulverized product with a jet mill to fine particles having an average particle diameter of 10 μm or less, wherein pulverization treatment time in the first pulverization process is determined to be 40 hours or more. | 09-04-2008 |
20080269463 | Tropoelastin Isoforms and Used Thereof - This disclosure provides new isoforms of tropoelastin. The disclosure further provides methods for making and using these isoforms, alone or in combination with each other or other isomers, such as in the production of biomaterials. | 10-30-2008 |
20080293918 | METHOD AND APPARATUS FOR EXTRACTION OF PROTEIN MATERIAL FROM ARTHROPOD GLANDS AND A MEMBRANE FORMED THEREFROM - An apparatus and method for the extraction of material from glands of arthropods. The apparatus comprises a container in which at least part of the glands are placed and a buffer solution at least partially immersing the glands. Gland material is collected in the material collection area of container. In use the material is released from glands into the buffer solution and sedimented at the bottom of the container. The method comprises: a first step of removing from a body of the arthropod the gland containing at least partially the material; a second step of making an opening in an epithelium of the gland; and a third step of placing the gland in a container at least partially immersed in a buffer solution such that the materials exit the glands and sediments in the material collection area of the container. | 11-27-2008 |
20090137781 | Method and Device for Producing a Thread from Silk Proteins - The present invention relates to a thread preparation process from silk proteins including an apparatus which is appropriate for performing the method. Furthermore, the invention is directed to the threads obtained therewith as well as the use thereof. The invention uses a diffusion unit leading to the production of high-quality silk threads with high yield. | 05-28-2009 |
20090286959 | High Molecular Weight Fibroin Having Improved Antioxidant Activity, Tyrosinase Inhibitory Ability and/or Cytotoxicity to Cancer Cells by Irradiation, and Methods of Making and Using the Same - Disclosed are a fibroin with increased molecular weight and improved antioxidant activity, tyrosinase inhibitory ability and cytotoxicity to cancer cells, which has a molecular structure modified by irradiation, a method for production thereof, use of the irradiated fibroin in various applications for enhancing antioxidant activity, tyrosinase inhibitory ability and/or cytotoxicity to cancer cells such as foods, cosmetics, medicines, etc. | 11-19-2009 |
20100222553 | SPIDER SILK DRAGLINE POLYNUCLEOTIDES, POLYPEPTIDES AND METHODS OF USE THEREOF - The disclosure provides spider silk dragline polypeptides and polynucleotides encoding the same. Methods of using such polypeptides and polynucleotides and designing novel biomaterials using repeat units of the polypeptides and polynucleotides. | 09-02-2010 |
20110021752 | Tropoelastin Derivatives - The invention relates to derivatives of tropoelastin and variants of those derivatives. The invention further provides expression products and hybrid molecules of the derivatives and variants of the invention. The invention further provides methods for the production of the derivatives, variants, expression products and hybrid molecules. Further provided are formulations, cross-linked structures and implants comprising the derivatives, variants, expression products and hybrid molecules of the invention. Further provided are uses of the derivatives, variants, expression products and hybrid molecules of the invention. | 01-27-2011 |
20110172394 | IMPLANTABLE MATERIAL FOR THE REPAIR, AUGMENTATION, OR REPLACEMENT OF BONE AND A METHOD FOR THE PREPARATION THEREOF - A method for the preparation of an implantable material for the repair, augmentation or replacement of bone from a fibroin solution, the method comprising the steps of: preparing a gel from the fibroin solution; preparing a material by subjecting the gel to one or more steps of freezing and thawing the gel, wherein the step of preparing the gel from the fibroin solution is performed in the presence of phosphate ions. The material may be treated with calcium ionstoform a fibroin-apatite. A further method comprises the step of treating the material with an isocyanate. The invention also extends to a method for the preparation of an implantable material, wherein a regenerated fibroin solution is used. Also, there is an implantable material and an implant. | 07-14-2011 |
20110201783 | Apparatus for the Preparation of a Thread From Silk Proteins - The present invention relates to a thread preparation process from silk proteins including an apparatus which is appropriate for performing the method. Furthermore, the invention is directed to the threads obtained therewith as well as the use thereof. The invention uses a diffusion unit leading to the production of high-quality silk threads with high yield. | 08-18-2011 |
20110288273 | HIGH YIELD DIALYSIS-FREE PROCESS FOR PRODUCING ORGANOSOLUBLE REGENERATED SILK FIBROIN - The invention relates to a simple and high yield process for producing a regenerated silk fibroin which does not need dialysis. Particularly, a process of the invention is characterized in that the silk fibroin is precipitated by applying a shear stress and/or changing the solvent power of the fibroin solution. The process of the invention simplifies the process of producing silk fibroin and greatly shortens the process time to 1 to 2 hours, whereas the conventional dialysis process is complex and needs around 2 to 3 days. In addition to reducing the time needed, the process of the invention can increase productivity of silk fibroin by at least 8%. | 11-24-2011 |
20120041177 | METHOD OF PRODUCING POLYMERS OF SPIDER SILK PROTEINS - A method of producing polymers of an isolated spider silk protein involves providing a solution of said spider silk protein in a liquid medium at pH 6.4 or higher and/or an ion composition that prevents polymerisation of the spider silk protein. The properties of the liquid medium are adjusted to a pH of 6.3 or lower and an ion composition that allows polymerisation of the spider silk protein. The spider silk protein is allowed to form polymers in the liquid medium, and the resulting spider silk protein polymers are isolated from the liquid medium. The resulting polymers are useful as fibers, films, foams, nets or meshes. | 02-16-2012 |
20120065375 | RECOMBINANT ORGANISM AND PROTEIN PRODUCED BY THE RECOMBINANT ORGANISM - The present invention relates to a recombinant organism having any one of nucleic acids (i) to (iv) introduced therein: (i) a nucleic acid having a base sequence of SEQ ID NO: 1; (ii) a nucleic acid encoding a protein having an amino acid sequence of SEQ ID NO: 2; (iii) a nucleic acid encoding a dragline protein and having a sequence identity of 90% or more with the nucleic acid (i); (iv) a nucleic acid which encodes a dragline protein and hybridizes with a complementary chain of the nucleic acid (i) under stringent conditions. | 03-15-2012 |
20120130046 | TROPOELASTIN DERIVATIVES - The invention relates to derivatives of tropoelastin and variants of those derivatives. The invention further provides expression products and hybrid molecules of the derivatives and variants of the invention. The invention further provides methods for the production of the derivatives, variants, expression products and hybrid molecules. Further provided are formulations, cross-linked structures and implants comprising the derivatives, variants, expression products and hybrid molecules of the invention. Further provided are uses of the derivatives, variants, expression products and hybrid molecules of the invention. | 05-24-2012 |
20120142898 | IMPLANTABLE MATERIAL AND A METHOD FOR THE PREPARATION THEREOF - A method for the preparation of a regenerated silk fibroin solution comprises the steps of: treating silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C.; and subsequently degumming the treated silk or silk cocoons; or alternatively, degumming silk or silk cocoons; and subsequently treating the degummed silk or silk cocoons with an ionic reagent comprising an aqueous solution of monovalent cations and monovalent anions, the cations and anions having ionic radii of at least 1.05 Angstroms and a Jones-Dole B coefficient of between −0.001 and −0.05 at 25° C. The invention also extends to fibroin solution, a fibroin material and an implant useful for cartilage repair. | 06-07-2012 |
20120184714 | COST-EFFECTIVE METHOD FOR EXPRESSION AND PURIFICATION OF RECOMBINANT PROTEINS IN PLANTS - Downstream purification of recombinant proteins from plant-based samples is performed by elastin-like polypeptides (ELP)-intein fusion expression and purification through inverse phase transition of ELP and cleavage reaction of intein. | 07-19-2012 |
20120245326 | SPIDER MITE SILK PROTEINS - Described are silk proteins derived from spider mite, more specifically derived from | 09-27-2012 |
20120264914 | SPIDER SILK PROTEINS AND METHODS FOR PRODUCING SPIDER SILK PROTEINS - The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof. | 10-18-2012 |
20120302734 | Processes for Producing Silk Dope - The present invention relates to methods of producing silk dope comprising silk proteins with a coiled-coil structure such as honeybee silk proteins. The silk proteins are obtained from cells producing them, solubilising the proteins by contacting them with a surfactant or an ionic liquid and concentrating the proteins to produce silk dope. The proteins can be used for a variety of purposes such as in the production of personal care products, plastics, textiles and biomedical products. | 11-29-2012 |
20120329992 | SPIDER SILK PROTEINS AND METHODS FOR PRODUCING SPIDER SILK PROTEINS - The invention provides an isolated major ampullate spidroin protein, which consists of from 150 to 420 amino acid residues and is defined by the formula REP-CT. REP is a repetitive, N-terminally derived protein fragment having from 80 to 300 amino acid residues. CT is a C-terminally derived protein fragment having from 70 to 120 amino acid residues. The invention further provides an isolated fusion protein consisting of a first protein fragment, which is a major ampullate spidroin protein, and a second protein fragment comprising a fusion partner and a cleavage agent recognition site. The first protein fragment is coupled via said cleavage agent recognition site to the fusion partner. The invention also provides a method of producing a major ampullate spidroin protein and polymers thereof. | 12-27-2012 |
20130060008 | METHOD FOR SILK FIBROIN GELATION USING SONICATION - This invention provides for a process of rapidly forming silk fibroin gelation through ultrasonication. Under the appropriate conditions, gelation can be controlled to occur within two hours after the ultrasonication treatment. Biological materials, including viable cells, or therapeutic agents can be encapsulated in the hydrogels formed from the process and be used as delivery vehicles. | 03-07-2013 |
20130109836 | SILK FIBROIN POROUS MATERIAL AND METHOD FOR PRODUCING SAME | 05-02-2013 |
20130109837 | METHODS OF MAKING A DIAGNOSTIC COMPOSITION AND COMPOSITIONS THEREFROM | 05-02-2013 |
20130178602 | NUCLEIC ACID ENCODING A SILK PROTEIN AND A METHOD OF PRODUCING A SILK THREAD USING SAME - Disclosed are a protein encoded by any one of nucleic acids (i) to (iv): (i) a nucleic acid having a base sequence of SEQ ID NO: 1; (ii) a nucleic acid encoding a protein having an amino acid sequence of SEQ ID NO: 2; (iii) a nucleic acid encoding a dragline protein and having a sequence identity of 90% or more with the nucleic acid (i); (iv) a nucleic acid which encodes a dragline protein and hybridizes with a complementary chain of the nucleic acid (i) under stringent conditions, and a silk thread containing the protein. | 07-11-2013 |
20130197193 | METHOD FOR PRODUCING POROUS SILK FIBROIN MATERIAL - A method for producing a porous silk fibroin material, containing steps of: freezing a silk fibroin solution containing a silk fibroin aqueous solution having an aliphatic carboxylic acid added thereto; and then melting the frozen solution, thereby providing a porous material. A method for producing a porous silk fibroin material having excellent mechanical characteristics may be provided. | 08-01-2013 |
20130197194 | SPIDER SILK BASED MATRIX - A silk fiber based matrix composition comprising spider silk that can be biodegradable, from the spider species | 08-01-2013 |
20130267682 | DRAGLINE PROTEIN - To provide a new natural fiber material with excellent physical properties. Any one of the following nucleic acids (a) to (d): (a) a nucleic acid having a base sequence of SEQ ID NO: 1 or 19; (b) a nucleic acid encoding a protein having an amino acid sequence of SEQ ID NO: 2 or 20; (c) a nucleic acid encoding a dragline protein and having a sequence identity of 90% or more with the nucleic acid (a); (d) a nucleic acid which encodes a dragline protein and hybridizes with a complementary chain of the nucleic acid (a) under stringent conditions. | 10-10-2013 |
20130281668 | Paralisation of Silkworm Larvae - The present disclosure relates to a method and use for immobilising Lepidopteran larvae and a method, device and use for forced silking from an immobilised Lepidopteran larva. A method for immobilising Lepidopteran larvae is disclosed which comprises paralysing the Lepidopteran larvae by at least one of transgenically expressing a polypeptide having paralytic effects in the Lepidopteran larvae, injecting hemolymph, wherein the hemolymph is drawn from Lepidopteran larvae and oxidised prior to the injecting, or injecting a natural or artificial polypeptide having paralytic effects. | 10-24-2013 |
20130310538 | METHODS AND COMPOSITIONS FOR DELIVERING ACTIVE AGENTS WITH ENHANCED PHARMACOLOGICAL PROPERTIES - Provided herein are methods of enhancing in vivo efficacy of an active agent, comprising: administering to a subject an active agent that is coupled to a bioelastic polymer or elastin-like peptide, wherein the in vivo efficacy of the active agent is enhanced as compared to the same active agent when administered to the subject not coupled to (or not associated with) a bioelastic polymer or ELP. | 11-21-2013 |
20140039159 | ELECTROACTIVE BIOPOLYMER OPTICAL AND ELECTRO-OPTICAL DEVICES AND METHOD OF MANUFACTURING THE SAME - A method of manufacturing a biopolymer optical device includes providing a polymer, providing a substrate, casting the polymer on the substrate, and enzymatically polymerizing an organic compound to generate a conducting polymer between the provided polymer and the substrate. The polymer may be a biopolymer such as silk and may be modified using organic compounds such as tyrosines to provide a molecular-level interface between the provided bulk biopolymer of the biopolymer optical device and a substrate or other conducting layer via a tyrosine-enzyme polymerization. The enzymatically polymerizing may include catalyzing the organic compound with peroxidase enzyme reactions. The result is a carbon-carbon conjugated backbone that provides polymeric “wires” for use in polymer and biopolymer optical devices. An all organic biopolymer electroactive material is thereby provided that provides optical functions and features. | 02-06-2014 |
20140058066 | ARTIFICIAL POLYPEPTIDE FIBER AND METHOD FOR PRODUCING THE SAME - An artificial polypeptide fiber of the present invention is an artificial fiber containing a polypeptide as a main component, and has a stress of 350 MPa or more and a toughness of 138 MJ/m | 02-27-2014 |
20140094589 | METHOD FOR PRODUCING WEB PROTEIN, A FUSED PROTEIN, RECOMBINANT DNA, AN EXPRESSION VECTOR, A HOST CELL AND STRAIN-PRODUCERS - The invention relates to the field of biotechnology and provides a method for producing recombinant proteins from the orb-weaving spider silk in yeast cells. This involves the construction of an expression vector which comprises a DNA sequence encoding a recombinant protein of the orb-weaving spider silk fused with a sequence encoding an ubiquitin-like protein occupying an N-terminal position with respect to the spider silk recombinant protein within the fused protein. The expression of a hybrid gene makes it possible to increase tens of times the production of recombinant spider silk protein, wherein the recombinant protein accumulates in the yeast cells in a water-insoluble fraction in the form of a processed protein free of a hybrid component. | 04-03-2014 |
20140303346 | METHOD FOR SILK FIBROIN GELATION USING SONICATION - This invention provides for a process of rapidly forming silk fibroin gelation through ultrasonication. Under the appropriate conditions, gelation can be controlled to occur within two hours after the ultrasonication treatment. Biological materials, including viable cells, or therapeutic agents can be encapsulated in the hydrogels formed from the process and be used as delivery vehicles. | 10-09-2014 |
20140378661 | MOLDED REGENERATED SILK GEOMETRIES USING TEMPERATURE CONTROL AND MECHANICAL PROCESSING - The present disclosure provides methods for fabricating various regenerated silk geometries using temperature control. In addition to temperature control, mechanical processing can be used to enhance properties of the fabricated article. The present disclosure also provides silk foam and paper-like materials molded using freezer processing. | 12-25-2014 |
20150038680 | SPIDER SILK DRAGLINE POLYNUCLEOTIDES, POLYPEPTIDES AND METHODS OF USE THEREOF - The disclosure provides spider silk polypeptides and polynucleotides encoding the same. Methods of using such polypeptides and polynucleotides and designing novel biomaterials using repeat units of the polypeptides and polynucleotides. | 02-05-2015 |
20150057435 | RECOMBINANT SILK PROTEIN DERIVED FROM SEA ANEMONES, METHOD FOR MANUFACTURING SAME, AND COMPOSITION FOR MANUFACTURING SILK FIBERS INCLUDING SAME - There are provided a recombinant silk protein derived from sea anemones, a method for producing the same, and a composition for preparing a silk fiber including same. The recombinant silk protein derived from sea anemones has sequence features similar to genetic information of a silk protein derived from spiders and silkworms. Also, a large amount of recombinant silk protein derived from sea anemones may be produced from a transformant and it has good physical properties such as strength and elasticity. Therefore, the recombinant silk protein derived from sea anemones can be usefully applied in various industrial fields in which natural silk protein can be applied, and it is expected to create new industrial fields based on its distinctive mechanical properties. | 02-26-2015 |
20150119554 | SPIDER SILK FUSION PROTEIN STRUCTURES INCORPORATING IMMUNOGLOBULIN FRAGMENTS AS AFFINITY LIGANDS - A recombinant fusion protein comprising the moieties Band CT, and optionally REP, wherein B is comprising at least one immunoglobulin fragment, which provides the capacity of selective interaction with an organic target; CT is a moiety of from 70 to 120 amino acid residues and is derived from the C-terminal fragment of a spider silk protein; and REP is a moiety of from 70 to 300 amino acid residues and is derived from the repetitive fragment of a spider silk protein. | 04-30-2015 |
20150141618 | SOLUTION-DYED PROTEIN FIBER AND METHOD FOR PRODUCING SAME - A solution-dyed protein fiber of the present invention includes 0-100 mass % of silk fibroin and 100-0 mass % of a polypeptide derived from spider silk proteins when the protein fiber is assumed to be 100 mass %, wherein the solution-dyed protein fiber contains a solution-dyeing colorant. The fiber is obtained by: dissolving or dispersing a solution-dyeing colorant in a solvent used for a spinning solution or in dimethyl sulfoxide, thereby preparing a coloring liquid; adding a solvent to the coloring liquid in an amount necessary for a spinning solution; adding and dissolving protein powder into the solvent, thereby preparing a spinning solution; and subjecting the spinning solution to wet spinning or dry-wet spinning. Thereby, the present invention provides a low-cost solution-dyed protein fiber in which a solution-dyeing colorant is dispersed uniformly and that can exhibit bright color tone, and a method for producing the same. | 05-21-2015 |
20150291673 | SPIDER SILK PROTEIN FILM, AND METHOD FOR PRODUCING SAME - A film of the present invention contains a polypeptide derived from spider silk proteins. The decomposition temperature of the film is 240 to 260° C. The film absorbs ultraviolet light having a wavelength of 200 to 300 nm and has a light transmittance of 85% or more at a wavelength of 400 to 780 nm. The film is transparent and colorless in a visible light region. A method for producing a film of the present invention includes: dissolving a polypeptide derived from spider silk proteins in a dimethyl sulfoxide solvent to prepare a dope; and cast-molding the dope on a surface of a base. Thus, the present invention provides a spider silk protein film that can be formed easily and has favorable stretchability, and a method for producing the same. | 10-15-2015 |
20150322121 | Chimeric Spider Silk Polypeptides and Fibers and Uses Thereof - Transgenic silkworms comprising at least one nucleic acid encoding a chimeric silk polypeptide comprising one or more spider silk elasticity and strength motifs are disclosed. Expression cassettes comprising nucleic acids encoding a variety of chimeric spider silk polypeptides (Spider 2, Spider 4, Spider 6, Spider 8) are also disclosed. A piggyBac vector system is used to incorporate nucleic acids encoding chimeric spider silk polypeptides into the mutant silkworms to generate stable transgenic silkworms. Chimeric silk fibers having improved tensile strength and elasticity characteristics compared to native silkworm silk fibers are also provided. The transgenic silkworms greatly facilitate the commercial production of chimeric silk fibers suitable for use in a wide variety of medical and industrial applications. | 11-12-2015 |
20150329587 | EXTRACTION METHOD FOR HYDROPHILIC RECOMBINANT PROTEIN - The present invention relates to a method for extracting a hydrophilic recombinant protein from a host expressing a hydrophilic recombinant protein. The method includes: a lysate acquisition step in which a dissolving solvent is added to the host expressing a hydrophilic recombinant protein so as to obtain a host cell lysate; and a step in which a diluting solvent is added to the host cell lysate obtained in the lysate acquisition step, an insoluble substance is separated from the obtained dilution, and a supernatant containing the hydrophilic recombinant protein is collected. The hydrophilic recombinant protein has a hydropathy index of 0 or less. The dissolving solvent is an aprotic polar solvent. The diluting solvent is an aqueous solvent. | 11-19-2015 |
20150337008 | METHOD FOR PRODUCING FIBROIN POWDER FROM SILK PRODUCTS OR FILAMENTS - Method for producing a fibroin powder from silk products or filaments without sericin and dyes having the following phases: a) introduction of products or filaments into a very tightly-woven sealed bag with high filtering capacity to allow the treatment liquid but not the silk fibrils to exit; b) introduction of the bag into a tank; with fresh and hot water with a mixer, in which soda is added to the water for a time of some hours; c) a rinsing phase of which the last is made with acetic acid in such a way as to make the fibre acceptable to the cutis as originally; d) phase of centrifugation and drying tumbler; e) tumbling phase, first opening the bags to throw away the material dried in the tumblers by pulverizing the fibre with a homogenous fragmentation in such a way as to keep the fibrils whole. | 11-26-2015 |