Entries |
Document | Title | Date |
20080269070 | METHOD FOR RAPIDLY SCREENING MICROBIAL HOSTS TO IDENTIFY CERTAIN STRAINS WITH IMPROVED YIELD AND/OR QUALITY IN THE EXPRESSION OF HETEROLOGOUS PROTEINS - The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like. | 10-30-2008 |
20080269071 | PSEUDOPTEROSIN-PRODUCING BACTERIA AND METHODS OF USE - Clonal strains of bacteria derived from | 10-30-2008 |
20080274913 | Multiplex Array Useful for Assaying Protein-Protein Interaction - The described invention shows how multiple interactions between two proteins of interest can be determined by observing activation or lack thereof of intracellular proteins, following interaction of ligand and receptor. | 11-06-2008 |
20080280781 | Methods and Compositions for Increasing Membrane Permeability - Methods and compositions for increasing membrane permeability are provided. One aspect provides protein resulting from a fusion between a membrane-active peptide and second peptide. Nucleic acids, and vectors encoding the, pore forming fusion proteins are also provided. | 11-13-2008 |
20080287317 | Yeast arrays, methods of making such arrays, and methods of analyzing such arrays - This patent describes a novel method of detecting genetic interactions in yeast. This method can also be used to screen for function of biological effectors on yeast. The method encompasses crossing yeast strains with genetic alterations to acquire double mutants. The phenotypes of these double mutants are then checked to detect genetic interactions between the double mutants. This method can be used to assign function to yeast genes and their viral, prokaryotic, and eukaryotic homologs, and aptamers. It can also be used to study yeast two hybrid interactions and to find genes that regulate certain yeast promoters. | 11-20-2008 |
20080312101 | Novel Phage Display Technologies - The present invention provides novel technologies for producing and screening fusion proteins on the surface of filamentous phage. In particular, a single vector can be used for generating cell and phage libraries containing a given series of protein sequences fused to either one or other of two phage coat proteins. This approach simplifies and improves the efficiency of the subsequent phage display-based selection of protein-binding molecules having therapeutic or diagnostic utility, such as antibodies, peptides, or epitope-binding regions. | 12-18-2008 |
20080318804 | Es Cell Mutation Method and System - This is intended to provide a technique for providing a stem cell having a mutation in both alleles (a pair of alleles). A method for producing a stem cell having a mutation in both chains of alleles which comprises: A) the step of providing a stem cell; B) the step of preventing Blm alleles from functioning in the stem cell; and C) the step of inducing mutation in the stem cell. It is also intended to provide a library of stem cells having a mutation in both chains of alleles wherein stem cells involved in the library have the mutation transferred thereinto over the entire genome. | 12-25-2008 |
20080318805 | Method and apparatus for producing high-yield tissue microarray blocks - A method and apparatus are provided for forming high-yield tissue microarray blocks capable of producing 1,000 or more replicate slides. In one embodiment, a plurality of donor tissue samples are minced and suspended in separate molten liquid carriers to form a plurality of separate two-phase mixtures of solid minced tissue and molten wax. Each of the two-phase mixtures is preferably formed into elongated columns and transferred into a separate well of a microarray block. A method and apparatus are provided for forming the elongated molten wax columns with minced tissue evenly distributed along the length of each column. The apparatus for forming the array includes an extrusion mechanism for transferring the columns of two-phase mixtures into the deep wells of the microarray block. The two-phase mixture may alternately be cooled and allowed to solidify before being transferred into the microarray block. An alternate embodiment is provided wherein cell cultures are utilized as starting material as opposed to solid donor tissue samples. | 12-25-2008 |
20090005264 | CELL SURFACE DISPLAY, SCREENING AND PRODUCTION OF PROTEINS OF INTEREST - Aspects of the invention provide compositions and methods for displaying engineered polypeptides on a cell surface. According to aspects of the invention, immobilized polypeptides can be screened to identify one or more variants having one or more functional or structural properties of interest. Aspects of the invention provide composition and methods for producing engineered protein or protein variants having a functional or a structural property of interest. | 01-01-2009 |
20090023600 | Device and Process for Measuring Cell Properties - The invention relates to a measuring device for measuring at least one property of cells or vesicles, characterized in that is divided into two chambers by a hydrophobic partition having a horizontal opening, these chambers containing electrolyte solution and said opening being closed by a lipid double layer. The invention is characterized in that at least one cell or vesicle contacts the lipid double layer. The invention also relates to methods involving the use of the measuring device and to arrays, measuring chambers and microtitration plates all suited therefor. | 01-22-2009 |
20090042740 | Novel Method for Cloning Variable Domain Sequences of Immunological Gene Repertoire - The present invention relates to a non-PCR (polymerase chain reaction) process, particularly a transcription-based amplification method, for amplifying and cloning sequences containing a variable domain sequence such as an immunoglobulin variable domain sequence from the immunological gene repertoire. The present invention contemplates the expression of antibody library in either in an in vivo expression vector or in an in vitro transcription/translation system. Isolation of a gene coding for a receptor having the ability to bind a preselected ligand and receptors produced by the gene isolated by the method is also contemplated. | 02-12-2009 |
20090082221 | Cross-Species and Multi-Species Display Systems - The present invention provides expression vectors and helper display vectors which can be used in various combinations as vector sets for multi-species and cross-species display of polypeptides on the outer surface of prokaryotic genetic packages and/or eukaryotic host cells. The multi-species and cross-species display systems can be practiced using the vector sets of the invention without having to change or reengineer the display vectors. The display systems of the invention are particularly useful for displaying a genetically diverse repertoire or library of polypeptides on the surface of phage, bacterial host cells, yeast cells, and mammalian cells. | 03-26-2009 |
20090093376 | Cellular microarray and its microfabrication method - A cellular microarray is disclosed, which has a substrate, multiple first conductive lines, multiple second conductive lines, and multiple PIREs arranged on the surface of the substrate in an array. Each PIRE includes multiple first ring-shaped electrodes, and multiple second ring-shaped electrodes. The first ring-shaped electrodes, and the second ring-shaped electrodes are located on the surface of the substrate alternately in each PIRE. Moreover, the outermost ring-shaped electrodes of any two adjacent feather-shaped electrodes are different. The disclosed cellular microarray can adhere the cells rapidly and uniformly, increase the output of manufacturing, and reduce the cost for manufacturing and application. | 04-09-2009 |
20090105090 | Phage Display By Novel Filamentous Bacteriophage - [Problems] To provide a phage display vector which can be fused in the inside of pIII and a phage display method using the vector. | 04-23-2009 |
20090137424 | Selective Posttranslational Modification of Phage-Displayed Polypeptides - The invention relates to posttranslational modification of phage-displayed polypeptides. These displayed polypeptides comprise at least one unnatural amino acid, e.g., an aryl-azide amino acid such asp-azido-L-phenylalanine, or an alkynyl-amino acid such as para-propargyloxyphenylalanine, which are incorporated into the phage-displayed fusion polypeptide at a selected position by using an in vivo orthogonal translation system comprising a suitable orthogonal aminoacyl-tRNA synthetase and a suitable orthogonal tRNA species. These unnatural amino acids advantageously provide targets for posttranslational modifications such as azide-alkyne [3+2]cycloaddition reactions and Staudinger modifications. | 05-28-2009 |
20090137425 | SUPPORTS FOR ASSAYING ANALYTES AND METHODS OF MAKING AND USING THEREOF - Described herein are supports for assaying an analyte and methods of making and using thereof. | 05-28-2009 |
20090163379 | Eukaryotic cell display systems - The present invention provides expression vectors and helper display vectors which can be used in various combinations as vector sets for display of polypeptides on the outer surface of eukaryotic host cells. The expression vector of the invention can be used alone for soluble expression without having to change or reengineer the display vectors. The display systems of the invention are particularly useful for displaying a genetically diverse repertoire or library of polypeptides on the surface of yeast cells, and mammalian cells. | 06-25-2009 |
20090170722 | Methods of constructing and screening diverse expression libraries - The present invention provides novel methods for producing nucleic acid fragment libraries that express highly diverse peptides or protein domains and, in particular, methods for producing nucleic acid fragment libraries wherein the nucleic acid fragments of the libraries are derived from two or more diverse characterized genomes. | 07-02-2009 |
20090203545 | EFFICIENT oriP/EBNA-1 PLASMID VECTOR - The invention provides a recombinant vector comprising a DNA segment having a synthetic origin of DNA synthesis that binds EBNA-1 and is capable of initiating DNA synthesis of sequences linked to the synthetic origin of DNA synthesis and maintaining the linked sequences when in the presence of EBNA-1. The synthetic origin of DNA synthesis comprises at least two binding sites for EBNA-1, wherein the two EBNA-1 binding sites are flanked by at least two half-binding sites for TRF2 or at least two binding sites for a protein that enhances the affinity of EBNA-1 for the synthetic origin of DNA synthesis. Further provided are host cells with the vector and methods of using the vector, for instance, ex vivo or in vivo. | 08-13-2009 |
20090264312 | METHOD FOR REMOVING DESIRED CHROMOSOME AND TAILOR-MADE MEDICAL TREATMENT UTILIZING THE SAME - It is intended to provide a regenerable cell in which a desired chromosome has been deleted, a method for producing the cell, and a gene cassette and a kit to be used for the method. More particularly, it is intended to obtain an individual corresponding pluripotent stem cell easily and simply. More specifically, it is intended to efficiently establish a cell, tissue or organ that can be a donor for treating a disease without causing immune rejection response, without newly obtaining and establishing a stem cell such as ES cell from the individual, and without using an ovum as a material. It was achieved by a gene cassette in which not two recombinase recognition sites in a cis orientation are inserted, but one recombinase recognition site or two recombinase recognition sites in an opposite orientation is/are inserted and a marker gene is connected, and by application of the gene cassette to a cell fusion technique. | 10-22-2009 |
20090264313 | Oligonucleotide library encoding randomised peptides - The invention relates to a method of producing an oligonucleotide library comprising a plurality of oligonucleotides, each oligonucleotide in the library having at least one predetermined position, a randomisation codon selected from a defined group of codons, the codons within said defined group coding for different amino acids. Vector, host cells containing such libraries and kits for the production of such libraries are also provided. | 10-22-2009 |
20090264314 | Optical Detection of Label-Free Biomolecular Interactions Using Microreplicated Plastic Sensor Elements - Methods and compositions are provided for detecting biomolecular interactions. The use of labels is not required and the methods can be performed in a high-throughput manner. The invention also provides optical devices useful as narrow band filters. | 10-22-2009 |
20100004139 | CONSTRUCTS AND LIBRARIES COMPRISING ANTIBODY SURROGATE LIGHT CHAIN SEQUENCES - The invention concerns constructs and libraries comprising antibody surrogate light chain sequences. In particular, the invention concerns constructs comprising VpreB sequences, optionally partnered with another polypeptide, such as, for example, antibody heavy chain variable domain sequences, and libraries containing the same. | 01-07-2010 |
20100009869 | ANTIBODY LIBRARIES - The present invention relates to the production of antibody libraries. In particular, the present invention relates to the use of integrating retroviral vectors to generate libraries comprising a plurality of combinations of antibody light chains and heavy chains. The present invention thus provides improved methods of generating and screening antibody libraries comprising large numbers of unique antibodies. | 01-14-2010 |
20100056394 | Mini Bio-Reactor - The present invention discloses a bio-reaction module, the so called mini bio-reactor, for providing a stable environment for the hybridization or immuno-reaction on biochips with constant temperature and humidity. Moreover, the mini bio-reactor can be a tool for studying the bio-reaction mechanism. Any variations in bio-reaction mechanism and environmental factors may affect experiment outcomes. Hence, when bulky equipment is miniaturized into a module system, the researchers can utilize a mini bio-reactor to perform bio-experiments or reactors in series connection to perform batch experiments. The structure design of mini reactor is able to promote better reaction efficiency and to shorten reaction time for a variety of bio experiments. Hence what is developed is an automatic, handy and inexpensive device for bio researches with high sensitivity and reliability. | 03-04-2010 |
20100062950 | CONSTRUCTS AND LIBRARIES COMPRISING ANTIBODY SURROGATE KAPPA LIGHT CHAIN SEQUENCES - The present invention concerns constructs and libraries comprising antibody surrogate κ light chain sequences. In particular, the invention concerns constructs comprising antibody surrogate κ light chain sequences, optionally partnered with another polypeptide, such as, for example, antibody heavy and/or light chain domain sequences, and libraries containing the same. | 03-11-2010 |
20100075868 | METHOD FOR DEVELOPING A TISSUE PROTEOME LIBRARY - The present invention relates to the description of an approach for developing tissue proteome library, which overexpresses all the transcripts (mRNAs) present in a given tissue. Transcripts of interest present in a tissue are normally cloned and overexpressed individually to enable purification of expressed protein and for conducting its structure-function studies. Methods for identification of novel and low abundant transcripts present in tissues are not available, particularly of specimen tissue samples, oocytes and early embryos, for which tissue availability is also a serious limitation. Expression of all the transcripts present in a tissue and comparison of the profile of total expressed protein with that of appropriate controls can be used in identification of all and particularly novel transcripts present in a tissue. This novel proteome library construction approach enables expression of all the transcripts present in a tissue just in one go and analysis of all the expressed proteins employing proteomics and/or other suitable approaches. | 03-25-2010 |
20100093562 | METAL COATED VIRUS-BASED NANOELECTRODES AND METHOD OF ASSEMBLING OF SAME - The present invention relates to high content surface areas containing nickel and/or cobalt metallic compounds assembled on a modified Tobacco mosaic virus (TMV) template, wherein the modified TMV template is engineered to encode unique placement of cysteine residues that self-assemble onto gold patterned surfaces in a substantially aligned fashion, producing a >10 fold increase in surface area. Deposition of ionic metals onto the surface assembled virus templates produce uniform metal coatings for the fabrication of oriented high surface area materials. | 04-15-2010 |
20100113302 | GENERATION OF LIBRARY OF SOLUBLE RANDOM POLYPEPTIDES LINKED TO mRNA - Methods and compositions are provided for producing libraries of soluble random polypeptides. In the methods, the fraction of hydrophilic residues in the polypeptide is controlled so as to maintain the solubility of the polypeptide constructs. | 05-06-2010 |
20100113303 | Polypeptide Display Libraries and Methods of Making and Using Thereof - Disclosed herein are expression vectors which display a passenger polypeptide on the outer surface of a biological entity. As disclosed herein the displayed passenger polypeptide is capable of interacting or binding with a given ligand. Also disclosed are methods of making and using the expression vectors. N/C terminal fusion expression vectors and methods of making and using are also disclosed. | 05-06-2010 |
20100113304 | COMPATIBLE DISPLAY VECTOR SYSTEMS - The present invention provides a polynucleotide vector system used during polypeptide display that can be used to facilitate transfer of pools of polynucleotides encoding antigen binding proteins of interest. The present invention also provides methods that allow seamless conversion of pools of polynucleotides encoding antigen binding proteins using a restriction enzyme digestion and ligation strategy. | 05-06-2010 |
20100120632 | Methods and Compositions For Sorting and/or Determining Organisms - This invention is directed to methods and compositions for sorting and/or determining microscopic organisms or cells. The methods and compositions are directed to the use of molecular probes to selectively stain the organisms or cells in combination with the use of binding partners to selectively immobilize the stained organisms or cells to a solid carrier. By combining the selectivity of both molecular probes and binding partners in an orthogonal method for staining and immobilization, these methods and compositions increase both the discriminating power of the assays and/or the certainty of the result obtained therefrom. | 05-13-2010 |
20100144548 | VECTOR SYSTEMS - The present invention relates generally to the field of molecular biology and genomics. More specifically, the present invention concerns the cloning of nucleic acid molecules and the production of nucleic acid libraries, as well as the expression of recombinant proteins and bactofection. | 06-10-2010 |
20100144549 | PARTHENOTE-DERIVED STEM CELLS AND METHODS OF MAKING AND USING THEM - Primate parthenotes, cells derived from them, and libraries of such cells are disclosed. Additionally, methods are disclosed for making primate parthenotes, the production of embryonic cells from these parthenotes, and for differentiating the partenotes into desired cell types, including multi-potent and differentiated cells. Methods are also provided for treating diseases or conditions for which the desired cell types are beneficial. Methods to identify agents of interest using these cells are also described. | 06-10-2010 |
20100179071 | CELL PATTERN AND METHOD FOR PRODUCING THE SAME - A method for producing a cell pattern is provided, comprising the step of forming a hydrophobic film on a substrate, patterning the hydrophobic film by a laser beam, transferring the hydrophobic film from the substrate to a medium, and culturing cells on the medium to form a cell pattern. | 07-15-2010 |
20100234243 | PTU COMPOUNDS FOR PROMOTING THE IN VITRO CULTURE OF (HIGHLY PIGMENTED) MELANOCYTES - A method for promoting the in vitro multiplication of human melanocytes and/or the freezing thereof, notably human melanocytes obtained from individuals of phototype IV, V or VI or from hyperpigmentary lesions, entails introducing into a culture of same, a thus effective amount of at least one 1-phenyl-3-(2-thiazolyl)-2-thiourea (PTU) compound or PTU analogue or PTU mimetic selected from the group consisting of vitamin C, arbutin, hydroquinone, kojic acid or acid or ester derivative thereof, ellagic acid, aminophenol derivative, procysteine or derivative thereof, niacinamide, isothiocyanate, thiocyanate, lucinol and mixtures thereof; also provided thereby can be melanocyte libraries, co-cultures and reconstructed epidermides and/or reconstructed skin that are highly pigmented which are useful for the study of pigmentation disorders, for the screening of cosmetic or dermatological active agents, or for the treatment of skin lesions, in particular in individuals with a high phototype. | 09-16-2010 |
20100234244 | USES AND METHODS OF MAKING MICROARRAYS OF POLYMERIC BIOMATERIALS - A microarray of polymeric biomaterials is provided. Specifically, a microarray of polymeric biomaterials that comprises a base with a cytophobic surface, and a plurality of discrete polymeric biomaterial elements bound to the cytophobic surface, is provided. Preferably said polymeric biomaterials comprise a synthetic polymer. Said polymeric biomaterials may also comprise other compounds covalently or non-covalently attached to said synthetic polymer. Methods of preparing the microarray of polymeric biomaterials of the present invention and uses of the microarray of polymeric biomaterials of the present invention are also provided. | 09-16-2010 |
20100248989 | Parthenogenic Activation of Human Oocytes for the Production of Human Embryonic Stem Cells - Methods of producing human stem cells are disclosed for parthenogenetically activating human oocytes by manipulation of O | 09-30-2010 |
20100279894 | BACTERIOPHAGES AND COATING MATERIAL FOR SURFACES - The present invention relates to a composition comprising bacteriophages and coating material for surfaces, characterized in that a first additional peptide is fused on proteins of the bacteriophage and furthermore a second additional peptide is fused on proteins of the bacteriophage. In addition, the present invention relates to a surface that has been coated with the composition and the use of the composition for coating surfaces. | 11-04-2010 |
20100285993 | Systematic Genomic Library and Uses Thereof - The present invention provides genomic DNA libraries that are systematically arranged on plasmids, methods of making and using the libraries, and plasmids that make up the libraries. | 11-11-2010 |
20100311612 | Compositions and Methods for Making Mutations in Cell Lines and Animals - The present invention is directed generally to reduction or inactivation of gene function or gene expression in cells in vitro and in multicellular organisms. The invention encompasses methods for mutating cells using a combination of mutagens, particularly wherein at least one mutagen is an insertional mutagen, to achieve homozygous gene mutation or mutation of multiple genes required cumulatively to achieve a phenotype to create knock-outs, knock-downs, and other modifications in the same cell. The invention is also directed to cells (and libraries thereof) and organisms created by the methods of the invention, including those in which at least one of the genes created by insertional mutagenesis is tagged by means of the insertion sequences thereby allowing identification of the mutated gene(s). The invention is also directed to libraries of mutated cells and their uses. The invention is also directed to methods of identifying mutations with methods of the invention, in cells (and libraries thereof) and organisms, by means of the insertional tag. | 12-09-2010 |
20100317546 | Display Vectors and Methods and Uses Thereof - The present invention relates, in one aspect, to a vector comprising (a) a first polynucleotide capable of encoding a first (poly)peptide comprising at least one cysteine residue, and (b) a second polynucleotide capable of encoding a second (poly)peptide which is a cell surface anchor comprising at least one cysteine residue, wherein the vector is operable in a eukaryotic host cell to express and to cause or allow the attachment of said first (poly)peptide to said second (poly)peptide by formation of a disulfide bond between said cysteine residues comprised within said first (polypeptide and said second (poly)peptide, respectively, wherein said first (poly)peptide is exhibited at the surface of a eukaryotic host cell. | 12-16-2010 |
20100323920 | ALLOGENEIC STEM CELL BANK - This invention provides methods for supplying a therapy for individuals exposed to radiation following a nuclear event, through the prospective establishment of an undesignated allogeneic stem cell bank with prospective HLA typing of healthy potential recipients. | 12-23-2010 |
20110003711 | CELL LINES EXPRESSING GABA RECEPTOR AND METHODS USING THEM - The invention relates to Gamma-aminobutyric acid receptors (GABA receptors) as well as cells and cell lines stably expressing a GABA receptor. The invention includes cell lines that express various subunit combinations of GABA receptors. The GABA receptor- and GABA receptor subunit-expressing cell lines are highly sensitive, physiologically relevant and produce consistent results. The invention further provides methods of making such cells and cell lines. The GABA receptor- and GABA receptor subunit-expressing cells and cell lines provided herein are useful in identifying modulators of GABA receptors. | 01-06-2011 |
20110009291 | METHODS AND COMPOSITIONS FOR INCREASING MEMBRANE PERMEABILITY - Methods and compositions for increasing membrane permeability are provided. One aspect provides protein resulting from a fusion between a membrane-active peptide and second peptide. Nucleic acids and vectors encoding the pore forming fusion proteins are also provided. | 01-13-2011 |
20110015094 | APPARATUS AND METHOD FOR EJECTING DROPLETS USING CHARGE CONCENTRATION AND LIQUID BRIDGE BREAKUP - Disclosed is an apparatus and method for ejecting droplets using charge concentration and liquid bridge breakup. The droplet ejection apparatus includes a reservoir storing a liquid; a capillary nozzle having a lower end submerged in the liquid stored in the reservoir and an upper end exposed outside the surface of the liquid, the capillary nozzle transferring the liquid to the upper end using capillary force; a potentiostat for applying a voltage to the liquid; a substrate mount on which a substrate is disposed to face the upper end of the capillary nozzle; and a distance adjusting unit for reciprocatingly moving the substrate between first and second positions with respect to the capillary nozzle, wherein the first position denotes a position where a distance between the upper end of the capillary nozzle and the surface of the substrate is less than a effective distance. | 01-20-2011 |
20110034348 | DEVICE FOR THE STUDY OF LIVING CELLS - A cell study device, comprising, a base layer, a planar conduit defining layer, including a conduit cut out of the layer; and a planar cover layer which defines a capillary flow channel in said conduit layer, said conduit layer and said cover layer acting as side walls for said capillary flow channel, wherein said layers are formed of materials that do not interfere with cell behavior over a period of at least 5 hours when loaded with aqueous solution. | 02-10-2011 |
20110053800 | METHOD OF MANUFACTURING PATTERNED SUBTRATE FOR CULTURING CELLS, PATTERNED SUBTRATE FOR CULTURING CELLS, PATTERNING METHOD OF CULTURING CELLS, AND PATTERNED CELL CHIP - The present invention relates to a method of manufacturing a patterned substrate for culturing cells, comprising the steps of: (1) preparing a substrate; (2) forming a first plasma polymer layer by integrating a first precursor material using a plasma on the substrate; (3) placing a shadow mask having a predetermined pattern on the first plasma polymer layer; and (4) forming a second patterned plasma polymer layer by integrating a second precursor material using a plasma. | 03-03-2011 |
20110082052 | PHAGE DISPLAYING SYSTEM EXPRESSING SINGLE CHAIN ANTIBODY - Disclosed are nucleic acid libraries for identifying a signal peptide that facilitates production of disulfide-stabilized single chain antibody, and for facilitating production of a disulfide-stabilized single chain antibody. Also disclosed are host cell libraries and phage libraries including the nucleic acid libraries. Further disclosed are methods for identifying a signal peptide that facilitates production of disulfide-stabilized single chain antibody, and methods for producing a disulfide-stabilized single chain antibody and non-fusion form thereof. | 04-07-2011 |
20110118146 | Phage Display Using Cotranslational Translocation of Fusion Polypeptides - The present invention relates to a filamentous phage display method wherein the polypeptides of interest displayed on the phage particle are cotranslationally translocated across the cytoplasmic membrane of Gram-negative bacteria based on the signal recognition particle pathway. This method is particularly suitable for polypeptides, which are known to be difficult to display on phages, and for proteins of cDNA libraries and other combinatorial libraries, in particular when derived from very fast folding, stable protein scaffolds. The invention further relates to phage or phagemid vectors useful in the method comprising a gene construct coding for a fusion polypeptide comprising the polypeptide to be displayed on the phage particle and an N-terminal signal sequence promoting cotranslational translocation. | 05-19-2011 |
20110118147 | LIBRARIES OF GENETIC PACKAGES COMPRISING NOVEL HC CDR3 DESIGNS - Provided are compositions and methods for preparing and identifying antibodies having CDR3s that vary in sequence and in length from very short to very long which in certain embodiments may bind to a carbohydrate moiety or the active site of an enzyme. Libraries coding for antibodies with the CDR3s are also provided. The libraries can be provided by modifying a pre-existing nucleic acid library. | 05-19-2011 |
20110130304 | METHOD FOR CREATING A VIRAL GENOMIC LIBRARY, A VIRAL GENOMIC LIBRARY AND A KIT FOR CREATING THE SAME - A method for creating an alphavirus-based genomic library, comprising a) ligation of foreign sequence (s) from an expression library or a random library into plasmids containing cloned alphaviral cDNA, b) multiplication of the obtained plasmid constructs in bacterial cells, c) direct transfection of the obtained plasmid constructs into mammalian or arthropod cells, characterized in that the sequence of an intron or sequences of introns are inserted into the respective genome of an alphavirus or into the cDNA of an expression vector based on an alphavirus, —the sequence of a viral subgenomic promoter, which is larger than minimal functional promoter is inserted immediately to the 3′ end of the sequences coding the structural proteins of the named alphavirus, —and ribozyme sequence is inserted for creating correct 3′ ends of the alphavirus. | 06-02-2011 |
20110152125 | Functional Porous Substrates For Attaching Biomolecules - A substrate comprising a microporous microstructure, an interlayer over at least a portion of the microstructure and a functional layer attached to the interlayer, the functional layer having functional sites with a density of at least 50 nanomoles/cm | 06-23-2011 |
20110172120 | Compositions and Systems for the Regulation of Genes - The present invention provides compositions and methods of modulating or regulating eukaryotic gene expression through the controlled or regulated expression of polynucleotide constructs that encode siRNA or other desired exogenous nucleic acids or proteins. Such constructs, and additional elements of the system may be transfected into the cells of interest and the expression of the siRNA, and hence the expression of the target gene of the siRNA, may be controlled through the administration of a compound to the cell, such as a small molecule or drug. Lentivirus vectors are employed in some embodiments of the invention including the generation of conditional knockdown animals. | 07-14-2011 |
20110172121 | ALLOGENEIC STEM CELL BANK - This invention provides methods for supplying a therapy for individuals exposed to radiation following a nuclear event, through the prospective establishment of an undesignated allogeneic stem cell bank with prospective HLA typing of healthy potential recipients. | 07-14-2011 |
20110224097 | VIABLE GRAM NEGATIVE BACTERIA LACKING OUTER MEMBRANE AGONISTS OF TLR4/MD-2 - Viable Gram-negative bacteria or components thereof comprising outer membranes that substantially lack a ligand, such as Lipid A or 6-acyl lipidpolysaccharide, that acts as an agonist of TLR4/MD2. The bacteria may comprise reduced activity of arabinose-5-phosphate isomerases and one or more suppressor mutations, for example in a transporter thereby increasing the transporters capacity to transport Lipid IVA or in membrane protein YhjD. One or more genes (e.g., IpxL, IpxM, pagP, IpxP, and/or eptA) may be substantially deleted and/or one or more enzymes (e.g., LpxL, LpxM, PagP, LpxP, and/or EptA) may be substantially inactive. The bacteria may be competent to take up extracellular DNA, may be donor bacteria, or may be members of a library. The present invention also features methods of creating and utilizing such bacteria. | 09-15-2011 |
20110237461 | USING PHAGE EPITOPES TO PROFILE THE IMMUNE RESPONSE - The present disclosure provides compositions and methods for using one or more polypeptide probes to profile an immune response. The polypeptide probe can be used to detect one or more antibodies from a sample. Furthermore, the present disclosure provides methods and compositions for characterizing a cancer based on the detection of one or more antibodies, such as autoantibodies. | 09-29-2011 |
20110245108 | Universal Antibody Libraries - Universal antibody libraries are described which are synthetic and derived from expressed human antibody sequences selected accordingly to certain criteria, for example, that the sequences are derived from naturally-occurring antibodies expressed in response to a certain antigen class (e.g., small molecule, polysaccharide, peptide, or protein) and having CDR regions engineered for optimal diversity. Methods for making and screening such libraries for isolating therapeutics suitable for treating disease are also disclosed. | 10-06-2011 |
20110251106 | PVII PHAGE DISPLAY - The present invention provides an alternative scaffold for peptides displayed on filamentous phages through novel fusion proteins primarily originating from pVII. Libraries of filamentous phages can be created from fusion proteins, and a phage display system comprising a phagemid and a helper phage is a part of the invention. An aspect of the invention is a kit containing a phage display system comprising a phagemid and a helper phage that contains a nucleic acid encoding the fusion protein of the invention. | 10-13-2011 |
20110257037 | MICROFLUIDIC DEVICE USING MICROFLUIDIC CHIP AND MICROFLUIDIC DEVICE USING BIOMOLECULE MICROARRAY CHIP - Disclosed is a microfluidic device including a microfluidic structure formed in a platform in which various examinations, such as an immune serum examination, can be automatically performed using the biomolecule microarray chip. The biomolecule microarray chip-type microfluidic device using a biomolecule microarray chip comprises: a platform which is rotatable; a microfluidic structure disposed in the platform, comprising: a plurality of chambers; a plurality of channels connecting the chambers each other; and a plurality of valves controlling flow of fluids through the channels, wherein the microfluidic structure controls flow of a fluid sample using rotation of the platform and the valves; and a biomolecule microarray chip mounted in the platform such that biomolecule capture probes bound to the biomolecule microarray chip contact the fluid sample in the microfluidic structure. | 10-20-2011 |
20110269645 | Cell chip, method of manufacturing the same and device for manufacturing cell chip - Provided is a cell chip, a method of manufacturing the same, and a device for manufacturing the same. The cell chip includes a substrate on which a plurality of cell fixing materials having a predetermined area are arranged, a plurality of single cells fixed by the plurality of cell fixing materials, respectively, and a plurality of cell scattering materials formed of a porous material and provided in the form of droplets combined with the plurality of cell fixing materials and surrounding the single cells, respectively. The device for manufacturing the cell chip includes a first inkjet head discharging a cell fixing material having a predetermined area onto a substrate, and a second inkjet head including an internal channel having one or more curved portions, and discharging a plurality of cells, contained in a cell scattering material passing through the internal channel, individually to the cell fixing material. | 11-03-2011 |
20110281764 | SCREENING ASSAYS AND METHODS - Screening assays and methods of performing such assays are provided. In certain examples, the assays and methods may be designed to determine whether or not two or more species can associate with each other. In some examples, the assays and methods may be used to determine if a known antigen binds to an unknown monoclonal antibody. | 11-17-2011 |
20110287978 | CELL CHIP - A cell chip is provided which includes a first substrate having a micro channel extending from an upper surface thereof to a lower surface or a side surface thereof, and a first bio matrix arranged on the upper surface of the first substrate to cover the micro channel while containing cells. The cell chip supplies fluid to cells contained in the bio matrix by means of perfusion and diffusion, thereby providing an environment similar to a biological environment. | 11-24-2011 |
20110287979 | Methods for Identifying and Isolating Cells Expressing a Polypeptide - The invention relates to novel polypeptides and cells comprising the polypeptides. The polypeptides and cells are used in methods to identify and/or isolate cells producing a protein with specific biological functions. In particular, the methods may be used for identifying, selecting, and isolating cells producing antigen-specific monoclonal antibodies. | 11-24-2011 |
20110301064 | SIGNAL SEQUENCE-INDEPENDENT PIX PHAGE DISPLAY - The present invention provides an alternative scaffold for peptides displayed on filamentous phages through novel fusion proteins primarily originating from pIX. Libraries of filamentous phages can be created from fusion proteins, and a phage display system comprising a phagemid and a helper phage is a part of the invention. An aspect of the invention is a kit containing a phage display system comprising a phagemid that contains a nucleic acid encoding the fusion protein of the invention and a helper phage. | 12-08-2011 |
20110312543 | Expression Vectors Based on Modified Ribosomal Protein Promoters and Uses Thereof in Post-Transcriptional Assessment - The present invention relates to expression vector comprising (a) a promoter region comprising a non-inducible constitutively active ribosomal protein gene promoter, (b) an operably linked reporter or gene sequence, and (c) a 3′ untranslated region (3′ UTR), which are suitable means for an selective assessment of post-transcriptional regulation, post-transcriptional control elements and factors as well as for identifying compounds that effect post-transcription. The present invention furthermore relates to arrays, expression vector libraries and cell lines containing the expression vector(s). The present invention furthermore relates to a method and kit for identifying compounds that affect post-transcriptional regulation of reporter(s) or gene(s), that utilize the expression vector(s). | 12-22-2011 |
20120021952 | FOCUSED LIBRARIES OF GENETIC PACKAGES - Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies. | 01-26-2012 |
20120028839 | SYNTHETIC IMMUNOGLOBULIN DOMAINS WITH BINDING PROPERTIES ENGINEERED IN REGIONS OF THE MOLECULE DIFFERENT FROM THE COMPLEMENTARITY DETERMINING REGIONS - Libraries of immunoglobulins which each have one or more amino acid modifications in at least one structural loop region of such immunoglobulins, where the modified loop region specifically binds to an epitope of an antigen to which an unmodified immunoglobulin does not significantly bind. | 02-02-2012 |
20120028840 | FOCUSED LIBRARIES OF GENETIC PACKAGES - Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies. | 02-02-2012 |
20120028841 | FOCUSED LIBRARIES OF GENETIC PACKAGES - Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies. | 02-02-2012 |
20120071354 | NORMALIZED NUCLEIC ACID LIBRARIES AND METHODS OF PRODUCTION THEREOF - The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods. | 03-22-2012 |
20120077710 | METHOD FOR AN IN VITRO MOLECULAR EVOLUTION OF ANTIBODY FUNCTION - The present invention relates to a method for in vivo molecular evolution of antibody function. According to the present invention, a nucleic acid encoding a CDR that is normally contained in a framework (the “original framework”), which differs from a selected master framework, is amplified from an immunoglobulin gene and is inserted into a nucleic acid encoding the selected master framework. The invention further provides an antibody library, such as a phage display library, and methods of making the same. | 03-29-2012 |
20120083428 | Method and Device for Protein Delivery into Cells - Methods for performing surface-mediated protein delivery into living cells, and fabricating protein-transfected cell cluster arrays are provided. The method comprises providing a protein-containing mixture; depositing said protein-containing mixture onto a surface at defined locations; affixing the protein-containing mixture to the surface as microspots; and plating cells onto the surface in sufficient density and under conditions for the proteins to be delivered into the cells. The protein-containing mixture comprises any suitable amino acid sequence, including peptides, proteins, protein-domains, antibodies, or protein-nucleic acid conjugates, etc., with a carrier reagent. Protein-transfected cell arrays may be used for rapid and direct, screening of protein or enzymatic functions or any given intracellular protein interaction in the natural environment of a living cell, as well as for high-throughput screening of other biological and chemical analytes, which affect the functions of these proteins. | 04-05-2012 |
20120094870 | Library of a Collection of Cells - The present invention relates to combinatorial gene expression libraries and methods for making these. Such libraries are useful in discovery of novel and/or enhanced metabolic pathways leading to the production of novel compounds for e.g., drug discovery and/or to the prosecution of known compounds in novel quantities or in novel compartments of the cells. The expression libraries in particular are composed of host cells capable of co-ordinated and controllable expression of large numbers of heterologous genes in the host cells. | 04-19-2012 |
20120108466 | IMMUNOGLOBULIN LIBRARIES - Methods and compositions for the screening and isolation of ligand-binding polypeptides, such as antibodies. In some aspects, methods of the invention enable the isolation of intact soluble antibodies comprising a constant domain. Screening methods that employ genetic packages such as bacteria and bacteriophages enable high through-put identification of ligand binding molecules. | 05-03-2012 |
20120122732 | Magnetic nanoparticles, magnetic detector arrays, and methods for their use in detecting biological molecules - Magnetic nanoparticles and methods for their use in detecting biological molecules are disclosed. The magnetic nanoparticles can be attached to nucleic acid molecules, which are then captured by a complementary sequence attached to a detector, such as a spin valve detector or a magnetic tunnel junction detector. The detection of the bound magnetic nanoparticle can be achieved with high specificity and sensitivity. | 05-17-2012 |
20120165225 | FUNCTIONAL BIOMOLECULES AND METHODS - The present invention is generally directed to novel functionalized biomolecules and methods for generating such biomolecules. Biomolecules may generally include nucleic acids, peptides, multicomponent molecular complexes and/or any other molecular products that may be produced by living organisms. The present invention is further directed to cells and/or organisms manipulated to produce such functionalized biomolecules. The cells contemplated by the present invention include both prokaryotic as well as eukaryotic cells. The functionalized biomolecules are produced via materials introduced into the cell using standard molecular biology techniques or are incorporated within the genomic nucleic acid of a cell by standard recombination techniques. Further contemplated is the use of such cells for sequestration of target molecules within the cells. | 06-28-2012 |
20120165226 | PHAGE DISPLAY - The transformation yield of electroporation is increased by using higher DNA concentrations and DNA affinity purification. Fusion proteins of a viral coat protein variant and a heterologous polypeptide are useful in phage display systems. | 06-28-2012 |
20120178649 | METHODS OF CONSTRUCTING AND SCREENING DIVERSE EXPRESSION LIBRARIES - The present invention provides novel methods for producing nucleic acid fragment libraries that express highly diverse peptides or protein domains and, in particular, methods for producing nucleic acid fragment libraries wherein the nucleic acid fragments of the libraries are derived from two or more diverse characterized genomes. | 07-12-2012 |
20120202710 | Methods and compositions for generation of germline human antibody genes - The present invention relates to a method for in vitro producing polynucleotides encoding human germline antibody V-regions. Also disclosed is a library of human germline antibody V-region genes. | 08-09-2012 |
20120214708 | Device and Process for Measuring Cell Properties - The invention relates to a measuring device for measuring at least one property of cells or vesicles, characterized in that is divided into two chambers by a hydrophobic partition having a horizontal opening, these chambers containing electrolyte solution and said opening being closed by a lipid double layer. The invention is characterized in that at least one cell or vesicle contacts the lipid double layer. The invention also relates to methods involving the use of the measuring device and to arrays, measuring chambers and microtitration plates all suited therefor. | 08-23-2012 |
20120220492 | Viral Vectors with Improved Properties - Methods to improve the tropism or other features of a virus are disclosed. Such methods can be used to prepare, e.g., DNA or plasmid libraries of variants of a gene encoding a viral capsid or envelope protein having a randomly inserted restriction site, libraries of viral clones with such variant genes with a randomly inserted restriction site or polypeptide sequence targeting a receptor expressed by a specific type of mammalian cells. Described are also methods to prepare mosaic viruses, i.e., viral particles wherein copies of one or more capsid or envelope proteins originate from different sources. These methods can be used to prepare mosaic viruses of a specific mixture of wild-type and mutant proteins, or of different types of mutant proteins. | 08-30-2012 |
20120264647 | DONOR SPECIFIC ANTIBODY LIBRARIES - The present invention concerns donor-specific antibody libraries derived from a patient donor who has suffered from, or is suffering from one or more diseases discussed herein. The present invention also concerns the method of making and using the donor-specific antibodies. The present invention further concerns the neutralizing antibodies obtained from the donor-specific antibody libraries and the methods of using these antibodies for the prevention/treatment of human disease. | 10-18-2012 |
20120264648 | In Vitro Model Of Spinal Muscular Atrophy - A population of iPS cells derived from somatic cells from a spinal muscular atrophy patient is disclosed. In one embodiment of the invention, the cells have been cultured to produce neural cells. In another embodiment, the invention is a method of testing compounds for their ability to modify cellular SMN levels comprising the steps of obtaining a population of iPS cells derived from a spinal muscular atrophy patient or cells derived from the iPS cells, and examining the effect of a test compound on SMN levels. | 10-18-2012 |
20120277120 | METHODS FOR GENOMIC MODIFICATION - Provided herein are methods of integrating one or more exogenous nucleic acids into one or more selected target sites of a host cell genome. In certain embodiments, the methods comprise contacting the host cell genome with one or more integration polynucleotides comprising an exogenous nucleic acid to be integrated into a genomic target site, and a nuclease capable of causing a double-strand break near or within the genomic target site. | 11-01-2012 |
20120302463 | FOCUSED LIBRARIES OF GENETIC PACKAGES - Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies. | 11-29-2012 |
20120309649 | CELL CHIP AND METHOD FOR MANUFACTURING THE SAME - Disclosed herein are a cell chip and a method for manufacturing the same. The cell chip may include: a substrate; and a first contact member disposed on the substrate, wherein a top end of the first contact member is provided with a first inclined contact surface inclined with respect to the substrate. | 12-06-2012 |
20120322690 | Anti-Rhesus D Recombinant Polyclonal Antibody and Methods of Manufacture - The invention relates to a method for manufacturing an anti-RhD recombinant polyclonal antibody composition (anti-RhD rpAb). The method comprises obtaining a collection of cells transfected with a library of anti-RhD antibody expression vectors, wherein each cell in the collection is capable of expressing from a VH and VL comprising nucleic acid segment, one member of the library, which encodes a distinct member of anti-RhD recombinant polyclonal antibody composition and is located at the same site in the genome of individual cells in said collection. The cells are cultured under suitable conditions for expression of the recombinant polyclonal antibody, which is obtained from the cells or culture supernatant. Nucleic acid segments encoding the anti-RhD rpAb are introduced into the cells by transfection with a library of vectors for site-specific integration. The method is suitable for manufacturing anti-RhD rpAb, thereby making available a superior replacement of plasma-derived prophylactic and therapeutic immunoglobulin products. | 12-20-2012 |
20130053278 | TOTIPOTENT, NEARLY TOTIPOTENT OR PLURIPOTENT MAMMALIAN CELLS HOMOZYGOUS OR HEMIZYGOUS FOR ONE OR MORE HISTOCOMPATIBILITY ANTIGENS GENES - The present invention relates to totipotent, nearly totipotent and pluripotent stem cells that are hemizygous or homozygous for MHC antigens and methods of making and using them. These cells are useful for reduced immunogenicity during transplantation and cell therapy. The cells of the present invention may be assembled into a bank with reduced complexity in the MHC genes. | 02-28-2013 |
20130102501 | DEVICE FOR RECOVERY AND ISOLATION OF BIOMOLECULES - The present invention relates to a device for isolating and recovering a biomolecule from a test sample. The device includes a support and at least one peelable layer deposited on at least a portion of the support. The peelable layer includes a substrate having a target component immobilized on the substrate. The device is effective for isolating and recovering a biomolecule having affinity to the target component. The present invention also relates to systems and methods of using the device. The present invention also relates to a biomolecule elution strip and related methods. | 04-25-2013 |
20130109593 | EXPRESSION OF MONOCLONAL ANTIBODIES IN CILIATE HOST CELLS | 05-02-2013 |
20130109594 | MICRO-ORGAN DEVICE | 05-02-2013 |
20130165347 | USE OF TERMINAL DEOXYNUCLEOTIDYL TRANSFERASE FOR MUTAGENIC DNA REPAIR TO GENERATE VARIABILITY, AT A DETERMINED POSITION IN DNA - The invention relates to a method of generating junctional variability in the nucleotide sequence of a polynucleotide of interest present in an intrachromosomal substrate/context in a eukaryotic cell which is competent for canonical Non Homologous End Joining pathway (NHEJ) repair, involving the generation of double-strand break (DSB) in the DNA sequence of said polynucleotide, and involving the use of polymerase Terminal Deoxynucleotidyl Transferase (TdT) in conditions enabling said TdT to add Non-templated nucleotides (N nucleotides) before ligation through the canonical Non Homologous End Joining pathway (NHEJ) thereby allowing a mutagenic repair to take place at the DSB site. The invention also relates to a library of eukaryotic cells and a collection of recombinant clones obtained by implementing the method of the invention on a population of eukaryotic cells, as well as a method for determining occurrence(s) of generation of double strand break(s) in a cell, or in a population of cells, after evaluation of the generated junctional variability. The invention further relates to the use of TdT as a marker of DSB events. | 06-27-2013 |
20130178396 | FOCUSED LIBRARIES OF GENETIC PACKAGES - Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies. | 07-11-2013 |
20130184183 | RECOMBINANT BACTERIOPHAGE AND METHODS FOR THEIR USE - Recombinant P4 bacteriophage containing modified tail fibers having a base plate attachment region (BPAR) from a P2 bacteriophage gene H product and a heterologous receptor binding domain (RBD) are disclosed. Methods for the use of the recombinant P4 bacteriophage, such as to detect the presence of a target bacterium in a sample, are also described. | 07-18-2013 |
20130190210 | ENGINEERED TISSUES FOR IN VITRO RESEARCH USES, ARRAYS THEREOF, AND METHODS OF MAKING THE SAME - Disclosed are living, three-dimensional tissue constructs for in vitro scientific and medical research, arrays thereof, and methods of making said tissues and arrays. | 07-25-2013 |
20130203630 | MODIFIED PEPTIDE DISPLAY - The invention provides a replicable genetic package displaying a cyclic peptide having at least one intramolecular bond between amino acid side chains. Also provided are a method of preparing such a genetic package displaying cyclic peptides having at least one intramolecular bond. Further provided is a library of replicable genetic packages displaying cyclic peptides each having at least one intramolecular cyclic bond between amino acid side chains; and a method of producing such a library. | 08-08-2013 |
20130210673 | Polypeptide Display Libraries and Methods of Making and Using Thereof - Disclosed herein are expression vectors which display a passenger polypeptide on the outer surface of a biological entity. As disclosed herein the displayed passenger polypeptide is capable of interacting or binding with a given ligand. Also disclosed are methods of making and using the expression vectors. N/C terminal fusion expression vectors and methods of making and using are also disclosed. | 08-15-2013 |
20130217597 | Compatible Display Vector Systems - The present invention provides a polynucleotide vector system used during polypeptide display that can be used to facilitate transfer of pools of polynucleotides encoding antigen binding proteins of interest. The present invention also provides methods that allow seamless conversion of pools of polynucleotides encoding antigen binding proteins using a restriction enzyme digestion and ligation strategy. | 08-22-2013 |
20130331297 | NOVEL PEPTIDES AND USES THEREOF - The present disclosure relates to novel peptides and their uses including, proline-rich peptides that are useful for displaying a protein of interest at the surfaces of a host cell such as a yeast. Polynucleotides, proteins, vectors and host cells that comprise or encode the novel proline-rich peptides, including libraries comprising such polynucleotides, proteins, vectors and/or host cells that comprise or encode novel proline-rich peptides are provided. Methods and materials for display and expression of proteins of interest are provided. Methods and materials are also provided by the present disclosure for isolating peptides capable of displaying a protein of interest (e.g., a marker protein), for generating libraries to display and/or express proteins of interest (e.g., antibodies such as humanized antibodies), for generating secretion vectors for such proteins of interest, and for generating proteins of interest (e.g., antibodies). | 12-12-2013 |
20130345094 | AUTOMATED SYSTEM FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS OR DIFFERENTIATED CELLS - The invention provides an automated system for producing induced pluripotent stem cells (iPSCs) from adult somatic cells. Further, the system is used for producing differentiated adult cells from stem cells. The invention system is useful for isolating somatic cells from tissue samples, producing iPSC lines from adult differentiated cells by reprogramming such cells, identifying the pluripotent reprogrammed adult cells among other cells, and expanding and screening the identified reprogrammed cells. | 12-26-2013 |
20140005075 | Composition for aggregating biological sample | 01-02-2014 |
20140005076 | Methods for Identifying and Isolating Cells Expressing a Polypeptide | 01-02-2014 |
20140018261 | FOCUSED LIBRARIES OF GENETIC PACKAGES - Focused libraries of vectors or genetic packages that display, display and express, or comprise a member of a diverse family of antibody peptides, polypeptides or proteins and collectively display, display and express, or comprise at least a portion of the focused diversity of the family. The libraries have length and sequence diversities that mimic that found in native human antibodies. | 01-16-2014 |
20140155294 | CELL CHIP, CELL SLICE SAMPLE AND MANUFACTURING METHOD THEREOF - There is provided a cell chip, a cell slice sample, and a manufacturing method thereof. The cell chip includes a plate member; biomaterials attached to one surface of the plate member; and a solidifiable material formed on one surface of the plate member to form a layer including the biomaterials. | 06-05-2014 |
20140162904 | Library of Cells That Produce Antibodies That Comprise Non-Naturally Paired IGH and IGL Variable Domains - A method for producing a library of engineered-antibody producing cells is provided. In certain cases, the method includes isolating nucleic acid sequences encoding IgH variable regions and IgL variable regions from a plurality of antibody producing cells, and introducing the nucleic acids into host cells to obtain cells that produce antibodies comprising non-naturally paired IgH and IgL variable chains. | 06-12-2014 |
20140162905 | EVAPORATIVE EDGE LITHOGRAPHY OF A LIPOSOMAL DRUG MICROARRAY FOR CELL MIGRATION ASSAYS - Lipid multilayer structures are formed by evaporating a solvent from each of a plurality of lipid solutions thereby form the lipid multilayer structures. Each lipid solution comprises the solvent and one or more lipids. Each lipid multilayer structure is a microstructure comprising one or more lipids. | 06-12-2014 |
20140179561 | HANGING DROP DEVICES, SYSTEMS AND/OR METHODS - The present disclosure relates general to devices, systems, and methods of using such devices in creating and handling hanging drops of fluid. The present disclosure also relates to cell culture devices, methods and/or systems of using such devices as well as the use of cell culture devices, for example, for research and high throughput screening. | 06-26-2014 |
20140206576 | UNIVERSAL FIBRONECTIN TYPE III BINDING-DOMAIN LIBRARIES - Walk-through mutagenesis and natural-variant combinatorial fibronectin Type III (FN3) polypeptide libraries are described, along with their method of construction and use. Also disclosed are a number of high binding affinity polypeptides selected by screening the libraries against a variety of selected antigens. | 07-24-2014 |
20140221250 | Rationally Designed, Synthetic Antibody Libraries and Uses Therefor - The present invention overcomes the inadequacies inherent in the known methods for generating libraries of antibody-encoding polynucleotides by specifically designing the libraries with directed sequence and length diversity. The libraries are designed to reflect the preimmune repertoire naturally created by the human immune system, with or without DH segments derived from other species, and are based on rational design informed by examination of publicly available databases of antibody sequences. | 08-07-2014 |
20140221251 | VIABLE GRAM NEGATIVE BACTERIA WITH REDUCED PROTEOLYTIC ACTIVITY LACKING OUTER MEMBRANE AGONISTS OF TLR4/MD-2 - Viable Gram-negative bacteria or components thereof comprising outer membranes that substantially lack a ligand, such as Lipid A or 6-acyl lipidpolysaccharide, that acts as an agonist of TLR4/MD-2. The bacteria may comprise reduced activity of arabinose-5-phosphate isomerases and one or more suppressor mutations, for example in a transporter thereby increasing the transporter's capacity to transport lipid IVA or in membrane protein YhjD. One or more genes (e.g., lpxL, lpxM, pagP, lpxP, and/or eptA) may be substantially deleted and/or one or more enzymes (e.g., LpxL, LpxM, PagP, LpxP, and/or EptA) may be substantially inactive. The bacteria may be competent to take up extracellular DNA, may be donor bacteria, or may be members of a library. The present invention also features methods of creating and utilizing such bacteria. | 08-07-2014 |
20140274800 | TRANSCRIPTION FACTOR DECOYS FOR THE TREATMENT AND PREVENTION OF INFECTIONS CAUSED BY BACTERIA INCLUDING CLOSTRIDIUM DIFFICILE - Decoy nucleic acid sequences comprising a binding site for a target transcription factor, wherein the binding site is not operably linked to a gene, and wherein the transcription factor comprises a regulator of expression of a gene or genes in | 09-18-2014 |
20140274801 | COMPOSITIONS, METHODS, AND COMPUTER SYSTEMS RELATED TO MAKING AND ADMINISTERING MODIFIED T CELLS - Embodiments described herein relate to methods, devices, and computer systems thereof for the derivation of T CAR libraries (Universal Subject or Individual Subject) for personalized treatment of disease in a subject. In certain embodiments, differential screening of normal and diseased tissue expression data is utilized to determine disease-specific antigens and thereby generate T CAR cells reactive to such antigens to form a disease-specific library. In certain embodiments, determination of the most effective T CAR clones from the disease-specific library is based on the subject's own disease-specific antigens. In certain embodiments, a subject is treated with a therapeutically effective amount of T CAR clones. | 09-18-2014 |
20140274802 | ENGINEERED LIVER TISSUES, ARRAYS THEREOF, AND METHODS OF MAKING THE SAME - Engineered, living, three-dimensional liver tissue constructs comprising: one or more layers, wherein each layer contains one or more liver cell types, the one or more layers cohered to form a living, three-dimensional liver tissue construct. In some embodiments, the constructs are characterized by having at least one of: at least one layer comprising a plurality of cell types, the cell types spatially arranged relative to each other to create a planar geometry; and a plurality of layers, at least one layer compositionally or architecturally distinct from at least one other layer to create a laminar geometry. Also disclosed are arrays and methods of making the same. Also disclosed are engineered, living, three-dimensional liver tissue constructs for use in the augmentation or restoration of one or more liver functions, by in vivo delivery of tissue or utilization of tissue in an extracorporeal device. | 09-18-2014 |
20140274803 | METHOD OF GENERATING GENE MOSAICS IN EUKARYOTIC CELLS - The invention relates to a method for generating a gene mosaic by somatic in vivo recombination, comprising a) in a single step procedure (i) transforming a cell with at least one gene A having a sequence homology of less than 99.5% to another gene to be recombined that is an integral part of the cell genome or presented in the framework of a genetic construct, (ii) recombining said genes, (iii) generating a gene mosaic of the genes at an integration site of a target genome, wherein said at least one gene A has a single flanking target sequence either at the 5′ end or 3′ end anchoring to the 5′ or 3′ end of said integration site, and b) selecting clones comprising the gene mosaic, wherein said cell is a eukaryotic strain with a knock-out of at least one DNA repair gene. The invention further refers to a method of producing a diversity of gene mosaics and gene assembly. | 09-18-2014 |
20140287960 | ENGINEERED LIVER TISSUES, ARRAYS THEREOF, AND METHODS OF MAKING THE SAME - Engineered, living, three-dimensional liver tissue constructs comprising: one or more layers, wherein each layer contains one or more liver cell types, the one or more layers cohered to form a living, three-dimensional liver tissue construct. In some embodiments, the constructs are characterized by having at least one of: at least one layer comprising a plurality of cell types, the cell types spatially arranged relative to each other to create a planar geometry; and a plurality of layers, at least one layer compositionally or architecturally distinct from at least one other layer to create a laminar geometry. Also disclosed are arrays and methods of making the same. Also disclosed are engineered, living, three-dimensional liver tissue constructs for use in the augmentation or restoration of one or more liver functions, by in vivo delivery of tissue or utilization of tissue in an extracorporeal device. | 09-25-2014 |
20140303037 | PATTERNING METHOD - A method of patterning a surface of a substrate comprising: (a) applying a coating to the surface to form a coated surface, and (b) treating the coated surface with a patterned microplasma comprising a plurality of localised microplasma discharges such that localised regions of the coated surface are selectively exposed to the localised microplasma discharges to form exposed localised regions and unexposed regions that have not been substantially exposed to a microplasma discharge; wherein the coating at the exposed localised regions is modified by the patterned microplasma and the coating at the unexposed regions is substantially unmodified to form a patterned surface on the substrate. | 10-09-2014 |
20140323353 | CELL-BASED ARRAYS, METHODS OF MAKING, AND METHODS OF USING - Embodiments of the present disclosure provide for arrays, systems, and methods analyzing cells, methods of making arrays, and the like. | 10-30-2014 |
20140364338 | Adeno-Associated Virus Virions with Variant Capsid and Methods of Use Thereof - The present disclosure provides adeno-associated virus (AAV) virions with altered capsid protein, where the AAV virions exhibit greater infectivity of retinal cells, when administered via intravitreal injection, compared to wild-type AAV. The present disclosure further provides methods of delivering a gene product to a retinal cell in an individual, and methods of treating ocular disease. | 12-11-2014 |
20150011427 | EVAPORATIVE EDGE LITHOGRAPHY OF A MICROARRAY - Patterned material depositions are formed on a surface of a substrate following filling each of one or more dispersions between each of spaces bounded by a stencil or a pair of patterned barriers on the surface of the substrate. Each dispersion comprises at least one liquid continuous medium and at least one dispersed material. Each patterned material deposition encompasses at least one dispersed material and is formed by evaporating the liquid continuous medium from the dispersion. Patterned material depositions may have multilayer structures. Systems include methods and device. | 01-08-2015 |
20150011428 | Array of Sensors with Surface Modifications - Surface modifications to sensors in an array give the sensors different functionalities for adsorbing or binding molecules. A first sensor in the array includes a first resonating member having a first surface comprising a polymer receptor material coated over a first underlying material. A second sensor includes a second resonating member having a second surface comprising the polymer receptor material coated over a second underlying material that is different than the first underlying material. The first underlying material, the second underlying material, and the polymer receptor material are selected such that the first resonating member, having a combination of the polymer receptor material and the first underlying material, has a different ability to adsorb or bind a mass of one or more analytes than does the second resonating member having a combination of the polymer receptor material with the second underlying material. Methods for fabricating sensors with surface modifications are also provided. | 01-08-2015 |
20150011429 | SERUM-FREE FREEZING MEDIUM USED IN ADIPOSE-DERIVED STEM CELLS AND ESTABLISHMENT OF ADIPOSE-DERIVED STEM CELL LIBRARY - Disclosed is a serum-free freezing medium used in adipose-derived stem cells and a method for establishing an adipose-derived stem cell library. The serum-free freezing medium comprises a serum-free culture medium, dimethyl sulfoxide and a serum substitution component KSR; the defects of unstable freezing quality of the adipose-derived stem cells and influence of harmful factors in serum on the adipose-derived stem cells are solved, and the adipose-derived stem cells stored have the advantages of high survival percentage, well adherence growth and strong differentiation capacity. | 01-08-2015 |
20150018248 | ARRAY PLATES FOR WASHING SAMPLES - Methods and systems for processing a first solution on a respective primary area of a device with a plurality of primary areas and a plurality of secondary areas are described. A method includes providing the device and dispensing a second solution to a respective secondary area of one or more secondary areas adjacent to the respective primary area, thereby mixing the first solution on the respective secondary area and the second solution on the respective primary area. The method also includes removing at least a portion of the mixed solution. | 01-15-2015 |
20150024971 | COMBINED AUTOMATED PARALLEL SYNTHESIS OF POLYNUCLEOTIDE VARIANTS - The present disclosure relates to methods for efficient synthesis, cloning, transformation and screening of large diverse libraries of polynucleotide variants comprising well-defined nucleotide differences relative to a reference polynucleotide. | 01-22-2015 |
20150031583 | PHAGE DISPLAY SYSTEM - A phage display system is provided comprising a mutant phage-infected host cell adapted to express a peptide of interest fused to a phage capsid protein, wherein the mutant phage includes a nonsense mutation which prevents expression of the capsid protein as a functional protein, and wherein expression of the peptide of interest is controlled by an inducible repressor and by a suppressor that suppresses the nonsense mutation. | 01-29-2015 |
20150057186 | PHAGE DISPLAY USING COTRANSLATIONAL TRANSLOCATION OF FUSION POLYPEPTIDES - The present invention relates to a filamentous phage display method wherein the polypeptides of interest displayed on the phage particle are cotranslationally translocated across the cytoplasmic membrane of Gram-negative bacteria based on the signal recognition particle pathway. This method is particularly suitable for polypeptides, which are known to be difficult to display on phages, and for proteins of cDNA libraries and other combinatorial libraries, in particular when derived from very fast folding, stable protein scaffolds. The invention further relates to phage or phagemid vectors useful in the method comprising a gene construct coding for a fusion polypeptide comprising the polypeptide to be displayed on the phage particle and an N-terminal signal sequence promoting cotranslational translocation. | 02-26-2015 |
20150298123 | ORGAN ON CHIP INTEGRATION AND APPLICATIONS OF THE SAME - A platform for cultivation, maintenance, and/or analysis of one or more bio-objects includes one or more integrated bio-object microfluidics modules. Each integrated bio-object microfluidics module is configured to cultivate, maintain, analyze and/or mimic functionalities of a respective bio-object, and includes one or more on-chip pumps; a plurality of fluidic switches; and a microfluidic chip in fluid communication with the one or more on-chip pumps and the plurality of fluidic switches, having at least one chamber for accommodating the bio-object and a plurality of fluidic paths connecting the at least one chamber, the one or more on-chip pumps and the plurality of fluidic switches, and a power and control unit adapted for selectively and individually controlling the one or more on-chip pumps and the plurality of fluidic switches for performing bio-object microfluidics functions. | 10-22-2015 |
20150315570 | DIRECTED EVOLUTION OF SYNTHETIC GENE CLUSTER - The invention relates to methods and products for generating diverse libraries of genetic material. The products include libraries and constructed nucleic acids as well as kits and databases and systems thereof. | 11-05-2015 |
20150322136 | BINDING POLYPEPTIDES WITH OPTIMIZED SCAFFOLDS - The invention provides variant heavy chain variable domains (VH) with increased folding stability. Libraries comprising a plurality of these polypeptides are also provided. In addition, compositions and methods of generating and using these polypeptides and libraries are provided. | 11-12-2015 |
20160010151 | MASSIVELY PARALLEL SINGLE CELL ANALYSIS | 01-14-2016 |
20160011176 | METHOD AND DEVICE FOR EXAMINING MYOCARDIAL TOXICITY AND EVALUATING CARDIOMYOCYTES | 01-14-2016 |
20160017368 | INORGANIC COATINGS FOR THE ENHANCEMENT OF CHEMICAL TRANSFECTION - Disclosed are methods for cell transfection and regulating cellular behavior. More particularly, the present disclosure relates to methods of non-viral cell transfection and regulating cellular behavior using mineral coatings that allow for the enhanced transfection of cells with reduced cytotoxicity. The mineral coatings bind biomaterials and provide a source of calcium and phosphate ions to enhance transfection. The present disclosure also provides a high throughput platform for screening non-viral transfection of cells. The methods of the present disclosure also provide an advantageous biomaterial delivery platform because the mineral coatings may be deposited on various medical device materials after being specifically developed using the high throughput screening platform. | 01-21-2016 |
20160024469 | Non-conventional Cellular Based Immunotherapy - The proposed patent is a method of immunotherapy wherein unique and novel sources of antigen generating organisms/viruses/cells are incubated or electroporated into further novel immunogenic cells. This method may be used for cancer therapy or therapy of benign disease. This method may also be used for immunization from infectious organisms such as bacteria, fungi, viruses or parasites. An aliquot of cells or materials obtained from the antigen generating component or electroporated immunogenic cells may be preserved long-term (i.e. cryopreservation or lyophilization or desiccation) for the purpose of forming a library or archive for future therapy or drug development/research. | 01-28-2016 |
20160031938 | PEPTIDE SELECTIVELY BINDING TO GRAPHITIC MATERIALS AND VOLATILE ORGANIC COMPOUNDS - Provided is a peptide including X | 02-04-2016 |
20160054302 | ARRAYS AND METHODS FOR GUIDED CELL PATTERNING - Guided cell patterning arrays for single cell patterning are disclosed. The arrays include a plurality of cell adhesion sites that are individually isolated on an inert surface. Each cell adhesion site has one or more cell adhesion peptides having affinity to a cell surface receptor. The inert surface is resistant to cell adhesion. | 02-25-2016 |
20160054314 | VIRUS-MICROBEAD COMPLEX AND USE THEREOF - The present invention relates to a virus-microbead complex including a microbead and a virus layer, in which linear viruses are bound individually to the surface of the microbead, and an immunoassay kit including the same. | 02-25-2016 |
20160060618 | IDENTIFYING AFFINITY-MATURED HUMAN ANTIBODIES - Antigen-specific immunoglobulin V-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all V-region sequences to be amplified (including those with extensive 5′ end mutations) without loss of the original 5′ V gene segment sequence. These libraries can be screened for antigen-specific V-regions using eukaryotic cells engineered to express the amplified V-region-encoding nucleic acids or using bacterial phage display techniques. In the latter, a second V-region library is made using a larger than conventional set of 5′ V-region primers. The sequence errors introduced into the amplification products by this method are corrected using sequence information obtained in the products amplified by the V-region primers to screen the library created using the leader sequence primers. Amino acid sequence information from fragments of donor immunoglobulins can be used to assist in the identification of nucleic acids encoding the heavy and light chains of donor antibodies as well as to design primers to amplify such nucleic acids. | 03-03-2016 |
20160060619 | IDENTIFYING AFFINITY-MATURED HUMAN ANTIBODIES - Antigen-specific immunoglobulin V-regions are identified from a library of nucleic acids amplified using polymerase chain reaction using leader sequence-specific forward primers. The use of leader sequence primers allows all V-region sequences to be amplified (including those with extensive 5′ end mutations) without loss of the original 5′ V gene segment sequence. These libraries can be screened for antigen-specific V-regions using eukaryotic cells engineered to express the amplified V-region-encoding nucleic acids or using bacterial phage display techniques. In the latter, a second V-region library is made using a larger than conventional set of 5′ V-region primers. The sequence errors introduced into the amplification products by this method are corrected using sequence information obtained in the products amplified by the V-region primers to screen the library created using the leader sequence primers. Amino acid sequence information from fragments of donor immunoglobulins can be used to assist in the identification of nucleic acids encoding the heavy and light chains of donor antibodies as well as to design primers to amplify such nucleic acids. | 03-03-2016 |
20160168542 | TISSUE ENGINEERED MODELS OF CANCERS | 06-16-2016 |
20160202208 | MULTIPLEXING DETECTION SYSTEM OF DUAL GATE ION-SENSITIVE FIELD-EFFECT TRANSISTOR SENSOR | 07-14-2016 |
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20190144851 | NOVEL PEPTIDES AND USES THEREOF | 05-16-2019 |
20190144952 | Gnotobiotic Rhizobacterial Isolation Plant Systems and Methods of Use Thereof | 05-16-2019 |