Entries |
Document | Title | Date |
20080207466 | System for high throughput GPCR functional assay - A functional assay detection system for membrane bound proteins. The system comprises a biological array including a porous substrate having a plurality of membranes adhered thereto and a first side and a second side, a fluorescent labeling reagent configured to couple to the membrane bound proteins, a pulsed light assembly configured to excite the fluorescent labeling reagent, and a time-delayed imaging device configured to capture emitted fluorescence of the fluorescent labeling reagent. The pulsed light assembly is configured to excite the fluorescent labeling reagent from at least one of the first side and the second side of the porous substrate, and the fluorescent labeling reagent comprises a fluorophore that has an emission lifetime that is in the range of microseconds. | 08-28-2008 |
20080220984 | Method for diagnosis of physiological states by detecting patterns of volatile analytes - Provided is a non-invasive method for identification of non-physiological, physiological or diseased states based on the volatiles in gas or biogas samples from individuals. The method uses a sensor array comprising a plurality of distinct sensors which differ from other sensors by the sensing molecules or the sol-gel holding material composition. In response to a combination of volatiles, a pattern of responses is generated which can be correlated to particular non-physiological, physiological or diseased state. | 09-11-2008 |
20080248967 | Process for Evaluating a Refinery Feedstock - A process for evaluating a refinery feedstock, said process comprising: (i) providing a refinery feedstock, (ii) treating said refinery feedstock to produce an array comprising a plurality of fractions having different chemical and/or physical properties, each fraction being representative of a process stream that might be present in a refinery, and (iii) analysing each of said plurality of fractions to determine one or more chemical and/or physical properties of the fractions, said analyses being performed at least partially in parallel. In a preferred embodiment, a plurality of refinery feedstocks is evaluated, each being fractionated prior to analysis of the fractions. | 10-09-2008 |
20080255001 | Screening of chemical compounds purified from biological sources - A method of producing a chemical compound library comprises extracting at least one extract from at least one species of plant; processing at least one of the extract(s) to remove at least one type of chemical interference to produce a processed extract; chromatographically separating the processed extract into a plurality of chromatographic fractions, each containing an amount of chemical compounds; determining the amount of chemical compounds in at least one of the chromatographic fractions; and normalizing the chromatographic fractions in which the amounts were determined to produce normalized chromatographic fractions, each such fraction comprising from about 1 microgram to about 500 micrograms of each of from one to seven chemical compounds that were present in lower concentrations in the extract and that each have a log P of from about −1 to about 5 and a molecular weight less than about 1000 Daltons; thereby to produce a chemical compound library from at least one species of plant. | 10-16-2008 |
20080261827 | Detection And Analysis System For Protein Array - To provide a method for detecting a protein by immobilizing a protein on a protein array substrate at a high density with controlled orientation, irradiating the immobilized protein with ultraviolet light, visible light, or infrared light, and measuring the light not absorbed by the protein and further analyzing an interaction between the protein on the substrate and another protein and/or a compound other than proteins, a system used for the method, and to provide a protein array suitable for the system. A system including a protein array in which a protein is immobilized in aligned position on a light-transmissive substrate at a high density, a light-irradiating means, and a light-detecting means and being for detecting or analyzing a protein on the protein array and/or a compound which is other than proteins and interacts with the immobilized protein, wherein the protein array is irradiated with light by the light-irradiating means, and the light transmitted through the protein array is measured by the light-detecting means for detecting the light absorption of the protein on the protein array and/or the compound which is other than proteins and interacts with the immobilized protein. | 10-23-2008 |
20080261828 | Quantum Dot Template for Fast and Simultaneous Detection of Different Infectious Agents - A method and a device for detecting the presence of a predetermined substance, in which a quantum dot is produced on a substrate. The quantum dot emits a radiation at a predetermined wavelength, and is covered with a surface layer to which the predetermined substance attaches. A deviation of the value of a parameter related to the radiation is produced when the predetermined substance attaches to the surface layer. This deviation can be detected to thereby sense the presence of the predetermined substance. | 10-23-2008 |
20080269069 | Method of Performing a Microarray Assay - Disclosed is a method for performing a microarray assay on one or more sample fluid(s), said fluids comprising target biological compounds. The method comprises the step of tagging said target biological compounds with labels. The following step comprises contacting said sample fluid(s) with a substrate and detecting the presence of said labels at the surface of said substrate. The method is suitable for the simultaneous analysis, in one microarray, of one or more types of target biological compounds, in one or more sample fluid(s). To this end each of said types of biological compounds is tagged with a different label so that target biological compounds belonging to different sample fluids have different labels. Said different labels are discriminable upon detection at the surface of said substrate. Also disclosed is the use of a polymer substrate in a method for performing a microarray assay. | 10-30-2008 |
20080287316 | Screening arrangement for screening immunoassay tests and agglutination tests - A screening device for performing an immunoassay test to detect the presence of a compound in a body fluid. The device includes a holder for removably receiving a membrane to which the fluid has been applied. A light is directed to the membrane. A photodetector measures the concentration of the light reflected back from the membrane. Specifically, the concentrations of reflected light from a control zone and a test zone are measured. Signals representative of the measured light concentrations are applied to a processor. If a specified concentration of predetermined light from a control zone on the membrane is detected, the processor considers the test to be successful. In the test is successful, the processor, based upon the measured concentration of reflected light from the test zone, generates data representative of the presence of the compound. | 11-20-2008 |
20080300146 | Bio Chip Device with a Sample Compartment and a Light Sensitive Element, Method for the Detection of Fluorescent Particles Within at Least One Sample Compartment of a Bio Chip Device - The invention provides a bio chip device comprising at least one sample compartment and at least one light sensitive element, the at least one sample compartment being provided on a first side of the at least one light sensitive element, wherein incident light is provided incident from a second side opposite of the first side of the at least one light sensitive element. Further, the invention provides a method for the detection of fluorescent particles within at least one sample compartment of a bio chip device. | 12-04-2008 |
20090011952 | Chip assay having improved efficiency - The chip includes electrodes on a substrate. The electrodes include a working electrode, a reference electrode, and a counter electrode. The reference electrode is constructed so as to not have an intrinsic potential. A self-assembly monolayer is positioned on the reference electrode. The self-assembly monolayer includes spacers and active probes. The active probes are configured to have a higher affinity for a capture probe than the spacers have for the capture probe. | 01-08-2009 |
20090042739 | CELLOMICS SYSTEM - In labeling a cell, and separating and collecting the cell according to a degree of the labeling using a cell separator, effects on the cell is minimized and the use of the collected cell is facilitated, thereby, when labeling a cell, the cell is labeled in the state where interaction of each cell is retained. In the labeling, a specific labeling material present on a surface of a target cell is taken in the cell via a transporter, and the cell is dispersed one by one to separate the same with a cell separator. Immediately after the separation, the cell is put in a solution not containing the specific labeling substance to remove the specific labeling substance taken in the cell. This series of steps is continuously conducted with a cell separation chip. | 02-12-2009 |
20090048122 | Stabilized polypeptide compositions - The invention is based, at least in part, on the development of stabilized binding molecules that consist of or comprise a stabilized scFv and methods for making such stabilized molecules. | 02-19-2009 |
20090054261 | METHOD - A method of controlling a system having a microfluidic channel structure in which fluids are able to interact to produce at least one product comprises the steps of:
| 02-26-2009 |
20090054262 | DEVICES FOR CELL ASSAYS - The present invention relates to the field of molecular diagnostics. In particular, the present invention provided improved substrates and methods of using liquid crystals and other biophotonically based assays for quantitating the amount of an analyte in a sample. The present invention also provides materials and methods for detecting non-specific binding of an analyte to a substrate by using a liquid crystal or other biophotonically based assay formats. | 02-26-2009 |
20090054263 | Grating waveguide structure for multi-analyte determinations and the use thereof - The invention relates to variable embodiments of a grating waveguide structure which enables to determine locally resolved changes of the resonance conditions for the incoupling of an excitation light into the waveguiding layer (a) of a stratified optical waveguide by means of a grating structure (c) modulated in said layer (a) or for outcoupling of a light guided in layer (a). The inventive system comprises arrays of measurement areas produced on the grating waveguide structure having different immobilized biological or biochemical or synthetic recognition elements for simultaneously binding and determining one or more analytes, wherein said excitation light is simultaneously irradiated onto an entire array of measurement areas, and the degree of satisfaction of the resonance condition for the incoupling of light into the layer (a) towards said measurement areas is simultaneously measured. The invention also relates to an optical system comprising at least one excitation light source and at least one locally resolving detector and, optionally, positioning elements for altering the angle of incidence of the excitation light onto the inventive grating waveguide structure. The invention additionally relates to a corresponding measuring method and to the use thereof. Surprisingly, it has been found that the inventive method is well-suited as an imaging detection method with high local resolution and sensitivity. | 02-26-2009 |
20090075837 | FRAMELESS MULTIPLEXED MICROARRAYS - The present invention relates to novel methods for the quantitative detection of molecules in an array. In particular, the present invention relates to methods and apparatuses for producing a frameless array. In another embodiment, the present invention relates to a composition comprising nitrocellulose that is useful of producing a frameless array. In another embodiment, the present invention relates to a method for detecting a molecular interaction. In yet another embodiment, the present invention relates to kits useful for practicing the methods and apparatuses of the present invention. The present invention provides improved methods and apparatuses for the high throughput analysis of molecular interactions and quantitative detection. | 03-19-2009 |
20090075838 | Biological Analysis Arrangement and Approach Therefor - Characteristics of a chemical or biological sample are detected using an approach involving light detection. According to an example embodiment of the present invention, an assaying arrangement including a light detector is adapted to detect light from a sample, such as a biological material. A signal corresponding to the detected light is used to characterize the sample, for example, by detecting a light-related property thereof. In one implementation, the assaying arrangement includes integrated circuitry having a light detector and a programmable processor, with the light detector generating a signal corresponding to the light and sending the signal to the processor. The processor provides an output corresponding to the signal and indicative of a characteristic of the sample. | 03-19-2009 |
20090082220 | Surface enhanced Raman spectroscopy (SERS) systems for the detection of bacteria and methods of use thereof - Surface-enhanced Raman spectroscopic (SERS) systems and methods for detecting biomolecules of interest, such as a bacterium or virus are provided. | 03-26-2009 |
20090088341 | MULTIPLEX ASSAY METHOD FOR MIXED CELL POPULATIONS - Disclosed is an in vitro assay for evaluating cellular activity, in which a culture is provided that contains one or more histologically different isolated mammalian cell-line that stably and constitutively express fluorescent proteins having a different emission spectra. The culture is assessed for cellular activity by quantifying fluorescence or detecting a pattern of fluorescence from fluorescent proteins present in the culture. In some embodiments, the cellular activity of the cell-lines is assessed by determining one or more of the growth rate, migration potential, or tubule formation potential of the cell-lines using the quantified fluorescence or pattern of fluorescence. Also disclosed are cell-lines have been stably transfected with mammalian expression plasmids that constitutively express different fluorescent proteins. Kits for performing the disclosed assays, which include the disclosed fluorescent cell-lines, are also disclosed. | 04-02-2009 |
20090088342 | SYSTEM, APPARATUS AND METHOD FOR APPLYING MECHANICAL FORCE TO A MATERIAL - The present invention details the design and operation of a miniaturized device array in which a range of simultaneous mechanical forces are produced by a single external pressure source. The invention is primarily embodied in a microfabricated device arrays designed to rapidly probe biological cell response to various combinations of mechanical, chemical and extra-cellular matrix parameters in a high-throughput fashion. | 04-02-2009 |
20090093375 | DNA or RNA detection and/or quantification using spectroscopic shifts or two or more optical cavities - A spectroscopic technique for high-sensitivity, label free DNA quantification uses a shift in an optical resonance (whispering gallery mode, WGM) excited in a micron-sized optical cavity (e.g., a silica sphere) to detect and measure nucleic acids. The surface of the silica sphere is chemically modified with oligonucleotides. Hybridization to the target DNA leads to a red-shift of the optical resonance wavelength. The sensitivity of this resonance technique is higher than most optical single-pass devices such as surface plasmon resonance biosensors. Each microsphere can be identified by its unique resonance wavelength. Specific, multiplexed DNA detection may be provided by using two or more microspheres. The multiplexed signal from two or more microspheres illustrates that a single nucleotide mismatch in an 11-mer oligonucleotide can be discriminated with a high signal-to-noise of 54. This all-photonic WGM biosensor can be integrated on a chip, such as a semiconductor chip, which makes it an easy to manufacture, analytic component for a portable, robust lab-on-a-chip device. | 04-09-2009 |
20090099038 | CELL LINE, SYSTEM AND METHOD FOR OPTICAL-BASED SCREENING OF ION-CHANNEL MODULATORS - A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor. | 04-16-2009 |
20090111711 | DEVICE AND METHOD FOR HIGH THROUGHPUT SCREENING OF CRYSTALLIZATION CONDITIONS IN A VAPOR DIFFUSION ENVIRONMENT - A high-density high-throughput microplate and methods for simultaneously screening a plurality of protein crystallization solutions and for producing diffraction quality protein crystals in a vapor-diffusion environment are disclosed. The microplate has defined side-by-side paired chambers of equal size, wherein the side-by-side paired chambers have a maximum volume of about 8 μl, and wherein the paired chambers have a vapor channel, therein providing vapor exchange between the side-by-side paired chambers. The microplate further includes a membrane to seal the surface of the microplate. The microplate is adapted to receive a crystallization solution in one of the side-by-side paired chambers and a protein solution in the other of the side-by-side paired chambers, wherein the protein solution and the crystallization solution interact via a vapor diffusion process, which enables the formation of protein crystals within the chamber that contains the protein solution. | 04-30-2009 |
20090131273 | Correlating spectral position of chemical species on a substrate with molecular weight, structure and chemical reactivity - A system for directly printing a variety of chemicals, including very large molecules on the substrate, includes a channel of nanometric dimension movable with respect to a substrate on which printing is to occur or a substrate movable with respect to the channel. Precision contact of the end aperture, or tip, of the channel with the surface deposits the chemical on the surface. Precision contact can be made by normal force atomic force microscopy or by other techniques that allow controlled contact or near contact with the surface on which the chemical is to be written with fine precision. Multiple channels with multiple orifices may be provided. The channel is connected to a suitable separation device such as a high performance liquid chromatograph and the chemicals are delivered through a probe orifice onto a substrate. The nanometric scale of the probe allows the chemicals to be printed on the substrate at spacings of from several nanometers to hundreds of micrometers in a fashion correlated with some external signal from a device that signals the ejection of a specific chemical. | 05-21-2009 |
20090131274 | DIAGNOSTIC NANOSENSOR AND ITS USE IN MEDICINE - This invention relates generally to biosensor technology, and pertains more particularly to novel multifunctional biosensors based on ordered arrays of metallic, semiconductors and magnetic nano-islands for medical, biological, biochemical, chemical and environmental applications. | 05-21-2009 |
20090137423 | Micro-electrode array - A method of fabricating a micro-electrode array comprising the steps of coating an electrode with an insulating polymer coating by screen printing an insulating polymer onto the electrode and then curing said polymer; and sonically ablating the insulating polymer coating to produce a plurality of micro-pores. | 05-28-2009 |
20090143244 | System and apparatus for sequential processing of analytes - An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array. A key feature of the invention is the correlation of the sequence of optical signals generated by each microparticle in the planar array during the analytical process. | 06-04-2009 |
20090143245 | MICROARRAYS FOR GENOTYPING AND METHODS OF USE - The present invention provides a microarray for detecting a genotype at a polymorphic site in a plurality of nucleic acid samples, comprising a first set of nucleic acid fragments derived from the samples and a second set of nucleic acid fragments derived from a plurality of references immobilized thereon. The invention also provides a microarray comprising a set of nucleic acid fragments immobilized on the surface of the microarray, wherein the nucleic acid fragments are derived from the samples by amplifying a region in the sample containing the polymorphism through asymmetric PCR amplification. Methods of using and making the microarrays are also provided. | 06-04-2009 |
20090149344 | SURFACE ENHANCED RAMAN SPECTROSCOPY (SERS) SYSTEMS AND METHODS OF USE THEREOF - Surface-enhanced Raman spectroscopic (SERS) systems and methods for detecting biomolecules of interest, such as a virus, bacterium, or other infectious agent, are provided. A spectroscopic assay based on surface enhanced Raman scattering (SERS) using a silver nanorod array substrate fabricated by oblique angle deposition has been developed that allows for rapid detection of trace levels of viruses or bacteria with a high degree of sensitivity and specificity. This novel and improved SERS assay can detect minor spectral differences within strains of a single virus type such as respiratory syncytial virus or influenza virus in the presence of biological media. The method provides rapid diagnostics for direct molecular and structural characterization of virus strains and virus gene deletion mutants generating reproducible viral spectra without viral manipulation. | 06-11-2009 |
20090149345 | MICROCHANNEL ARRAY AND METHOD FOR PRODUCING THE SAME, AND BLOOD MEASURING METHOD EMPLOYING IT - A microchannel array, a method of manufacturing the same, and a blood test method. The microchannel array is formed by joining first and second substrates, each including a fluid inlet and outlet on their surfaces. An internal space structure connects the fluid inlet and outlet, and includes an upstream flow channel connected with the fluid inlet, a downstream flow channel connected with the fluid outlet with a gap therebetween, and a micro flow channel connecting the upstream and downstream flow channels. A minimum distance from a center of a sectional surface of the micro flow channel to a side wall of the micro flow channel is smaller than that of the upstream and downstream flow channels. Each surface of the first and second substrates includes grooves for creating the upstream and downstream flow channels, and the surface of the second substrate has grooves for creating the micro flow channel. | 06-11-2009 |
20090156427 | Bio-Sensing Nanodevice - The invention provides a bio-sensing nanodevice comprising: a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—on a support, a real time receptor-ligand binding detection method, an odorant delivery system and an odorant recognition program. The biologically-derived G-protein coupled receptor can be stabilized on nanotechnology using surfactant peptide. The said nanodevice provides a greater surface area for better precision and sensitivity to odorant detection. The invention further provides a microfluidic chip containing a stabilized biologically-derived G-protein coupled receptor—the olfactory receptor—immobilized on a support, and arranged in at least two dimensional microarray system. The invention also provides a method of delivering odorant comprising the step of manipulating the bubbles in complex microfluidic networks wherein the bubbles travel in a microfluidic channel carrying a variety of gas samples to a precise location on a chip. The invention further provides method of fabricating hOR17-4 olfactory receptor. | 06-18-2009 |
20090156428 | MULTI-MODE MICROARRAY APPARATUS AND METHOD FOR CONCURRENT AND SEQUENTIAL BIOLOGICAL ASSAYS - A multi-mode, multiplexed array of probe elements of various forms attached to a common support and methods of using the array are disclosed. The array is used for the concurrent and/or sequential detection of combinations of more than one chemical and/or biological material, such as: target nucleic acid sequences, genomic DNA, pcr products, RNA (including microRNA), single nucleotide polymorphisms, proteins, peptides, carbohydrates, polysaccharides, phosphorylation or methylation state of target molecules, chromosomal abnormalities, and other biomolecules, moieties, metals, or chemical compounds in a biological sample. The method of the invention includes the deposition and/or in-situ synthesis of the various probe elements which comprise the array or group of arrays, the preparation and delivery of the target biological sample in forms suitable for concurrent and/or sequential detection, the hybridization methods of target to probe, the detection methods for target-probe interactions, and the use of such arrays in automated systems for research or diagnostic applications. | 06-18-2009 |
20090156429 | PULSED-MULTILINE EXCITATION FOR COLOR-BLIND FLUORESCENCE DETECTION - The present invention provides a technology called Pulse-Multiline Excitation or PME. This technology provides a novel approach to fluorescence detection with application for high-throughput identification of informative SNPs, which could lead to more accurate diagnosis of inherited disease, better prognosis of risk susceptibilities, or identification of sporadic mutations. The PME technology has two main advantages that significantly increase fluorescence sensitivity: (1) optimal excitation of all fluorophores in the genomic assay and (2) “color-blind” detection, which collects considerably more light than standard wavelength resolved detection. Successful implementation of the PME technology will have broad application for routine usage in clinical diagnostics, forensics, and general sequencing methodologies and will have the capability, flexibility, and portability of targeted sequence variation assays for a large majority of the population. | 06-18-2009 |
20090176661 | WHOLE GENOME EXPRESSION ANALYSIS SYSTEM - A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal. | 07-09-2009 |
20090186776 | Microcolumn-platform based array for high-throughput analysis - A device and methods for performing biological or chemical analysis is provided. The device includes an array of three-dimensional microcolumns projecting away from a support plate. Each microcolumn has a relatively planar, first surface remote from the support plate. An array of multiple, different biological materials may be attached to the first surface. The device, when used in combination with existent micro-titer well plates, can improve efficiency of binding assays using microarrays for high-throughput capacity. | 07-23-2009 |
20090215646 | SYSTEM AND METHOD OF ANALYTE DETECTION USING DIFFERENTIAL RECEPTORS - Methods and systems for detecting the presence of analytes are described. A fluid or gas sample containing one or more analytes may pass through a particle-based sensor array. Detection and analysis techniques may be applied to determine the identity and quantity of the analytes. | 08-27-2009 |
20090239767 | BIOMOLECULE DETECTION REAGENT AND METHOD FOR DETECTING BIOMOLECULE USING REAGENT - A biomolecule detection reagent comprising semiconductor nanoparticles and magnetic nanoparticles, incorporated in a bead comprising an inorganic compound or an organic polymer, and a surface of the beads is modified with a biomolecule detection molecule. | 09-24-2009 |
20090253589 | Method for Testing Active Compounds - In one aspect the invention relates to a method for testing a chemical entity for its capability to modulate a (poly)peptide that is malfunctioning by means of an interaction of said chemical entity and said (poly)peptide, the method using single-molecule force spectroscopy. In another aspect the invention relates to a method for testing a chemical or physical entity for its capability to interact with a G protein-coupled receptor (GPCR) in its natural membrane environment, the method using single-molecule force spectroscopy. | 10-08-2009 |
20090270275 | METHOD AND APPARATUS FOR SCANNING BIO CHIPS USING LIGHT AMPLICATION BY METAL NANO-PARTICLES - The present invention utilizes a principle in which, in a state in which metal nano-particles are attached to target probes and used as markers, and the metal nano-particles have a proper density according to a bio reaction between the target probes and fixed probes, when the metal nano-particles are irradiated with a laser beam having a proper intensity from the optical pick-up head, a higher optical energy is delivered to the phase change layer by an optical amplification effect caused by the metal nano-particles, thereby better inducing an amorphous-to-crystalline phase change. | 10-29-2009 |
20090318306 | SYSTEM AND METHOD FOR TEMPERATURE REFERENCING FOR MELT CURVE DATA COLLECTION - The present invention relates to systems and methods of temperature referencing for melt curve data collection. More specifically, the present invention relates to systems and methods for collecting DNA melt curve data for a DNA sample and a temperature reference material. | 12-24-2009 |
20090318307 | DEVICE FOR MOLECULAR DIAGNOSIS - The present invention relates to biological detection devices wherein melting curve analysis is performed an electrical sensor and a programmable heating element. The device optionally further comprises means for optically detecting nucleic acids within the device. | 12-24-2009 |
20100004137 | Characterization of biochips containing self-assembled monolayers - The present invention relates to a method of characterizing biochips with matrix-assisted laser desorption/ionization and time of flight mass spectrometry (MALDI-TOF MS). | 01-07-2010 |
20100004138 | Reagent for detecting biopolymer and method for detecting biopolymer - Detects the presence of binding between a sample biopolymer and a probe biopolymer and the amount of binding, without modifying the sample biopolymer in any way. The present invention provides a reagent for biopolymer detection comprising semiconductor nanoparticles on which a functional group having a positive or negative charge is exposed, and a method for biopolymer detection which detects the presence of binding between a sample biopolymer and a probe biopolymer and the amount of binding by electrostatically binding a semiconductor nanoparticle on which a functional group having a positive or negative charge is exposed to a negative or positive charge of the sample biopolymer. | 01-07-2010 |
20100009868 | Self-Assembled Combinatorial Encoding Nanoarrays for Multiplexed Biosensing - The present invention provides combinatorial encoding nucleic acid tiling arrays and methods for their use and synthesis. | 01-14-2010 |
20100035767 | POLYMERASE - The present invention relates to an engineered polymerase with an expanded substrate range characterized in that the polymerase is capable of incorporating an enhanced occurrence of detection agent-labeled nucleotide analogue into nucleic acid synthesized by that engineered polymerase as compared with the wild type polymerase from which it is derived. | 02-11-2010 |
20100048419 | Combinatorial Screening Method and Apparatus - A combinatorial screening method and system are provided. The combinatorial system and method provide rapid data generation for characterization of phase change material. The characterization data is collected through a multipoint probe card where multiple regions are characterized in a single annealing cycle. | 02-25-2010 |
20100056392 | MICROSTRUCTURE AND MICRODOMAIN MICROARRAYS, METHODS OF MAKING SAME AND USES THEREOF - Disclosed are methods for direct characterization of microdomains and/or three-dimensional microstructure arrays bearing high densities of reactive sites using Matrix Assisted Laser Desorption Ionization Time of Flight Mass Spectrometery (MALDI-MS) and other analytical techniques. The high site density of the arrays can provide sufficient sample of each array element and/or materials bound to each element to obtain directly using common analytical techniques such as MALDI-MS. Spatially directed synthesis of heteropolymers is done through the use of pliotolabile, electrically labile, and chemically labile protecting group(s). | 03-04-2010 |
20100056393 | APPARATUS AND METHOD FOR DETECTING A TARGET USING SURFACE PLASMON RESONANCE - Disclosed are substrates for detecting one or more target molecules and methods for detecting molecules using surface plasmon resonance. | 03-04-2010 |
20100062949 | FIBER OPTIC DEVICE - The present invention is directed to a fiber optic device consisting of a fiber bundle having multiple legs of silica fibers, using a plurality of microspheres construct to attach target cells, for the assay of cytotoxic compounds. Each leg of silica fibers consists of twenty-five or more silica fibers treated with biotin and streptavidin treated microspheres which chemically bind the microspheres to the silica fibers. Further, the present invention is directed to the unique microspheres. The microspheres have a core, preferably alginate, with an outer surface of chitosan. | 03-11-2010 |
20100069259 | SAMPLE DEVICE PRESERVATION - A method for archiving sample devices such as microarray slides and membranes is described using an optically clear, solidifying solution. Also described are related methods and kits. | 03-18-2010 |
20100081582 | HIGH THROUGHPUT METHOD FOR MEASURING TOTAL FERMENTABLES - According to the invention, there is provided a high throughput method for measuring total fermentables using a small amount of plan tissue. A high throughput screening tool for gene discovery aiming for increasing total fermentables is further provided. | 04-01-2010 |
20100105575 | SINGLE NUCLEOTIDE POLYMORPHISM GENOTYPING DETECTION VIA THE REAL-TIME INVADER ASSAY MICROARRAY PLATFORM - A method and apparatus for real-time, simultaneous, quantitative measurement for detecting a single nucleotide polymorphism in a target nucleic acid is provided. This method involves combining a polymerase chain reaction (PCR) technique with invader assay technique. | 04-29-2010 |
20100120629 | Biomarkers for Differentiating Between Type 1 and Type 2 Diabetes - Biomarkers that are diagnostic of type 1 diabetes, type 2 diabetes and/or diabetic disorder are identified. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of type 1 diabetes, type 2 diabetes and/or diabetic disorder. An analysis includes the parameters of matching for BMI and Tanner stage. Receiver-operator characteristic (ROC) curves were established to assess association of the biomarkers with a disease. | 05-13-2010 |
20100137162 | Method for Rapidly Screening Microbial Hosts to Identify Certain Strains with Improved Yield and/or Quality in the Expression of Heterologous Proteins - The present invention provides an array for rapidly identifying a host cell population capable of producing heterologous protein with improved yield and/or quality. The array comprises one or more host cell populations that have been genetically modified to increase the expression of one or more target genes involved in protein production, decrease the expression of one or more target genes involved in protein degradation, or both. One or more of the strains in the array may express the heterologous protein of interest in a periplasm compartment, or may secrete the heterologous protein extracellularly through an outer cell wall. The strain arrays are useful for screening for improved expression of any protein of interest, including therapeutic proteins, hormones, a growth factors, extracellular receptors or ligands, proteases, kinases, blood proteins, chemokines, cytokines, antibodies and the like. | 06-03-2010 |
20100210475 | MICROARRAY HAVING BRIGHT FIDUCIAL MARK AND METHOD OF OBTAINING OPTICAL DATA FROM THE MIRCOARRAY - A substrate includes; a fiducial mark disposed on the substrate, an area on the substrate on which a probe material is configured to be immobilized, the area being separated from the fiducial mark, and a probe immobilization compound disposed on the area on the substrate on which the probe material is configured to be immobilized, wherein the fiducial mark has a structure which reflects irradiated light at a greater intensity than an intensity of reflected irradiated light form the area on the substrate not corresponding to the fiducial mark. | 08-19-2010 |
20100216666 | METHOD OF PERFORMING A BIOLOGICAL ASSAY - The present invention relates to a method of pooling samples to be analyzed for a categorical variable, wherein the analysis involves a quantitative measurement of an analyte, said method of pooling samples comprising providing a pool of n samples wherein the amount of individual samples in the pool is such that the analytes in the samples are present in a molar ratio of x | 08-26-2010 |
20100216667 | METHOD FOR DETERMINING COMPATIBILITY OF AN ACTIVE PHARMACEUTICAL INGREDIENT WITH MATERIALS - Disclosed is a system or matrix approach for determining compatibility of a pharmaceutically active substance, such as small molecule drug candidate, therapeutic proteins, peptides, vaccines or RNAi, with materials used in the research and development of pharmaceuticals, including plastics, polymers, resins, rubbers, elastomers, glass and steel. | 08-26-2010 |
20100222235 | High throughput screening methods for fuel compositions - Methods for determining deposit formation tendencies for a plurality of fluid samples of different compositions is provided. Each sample includes fuel additive compositions containing one or more fuel additives or fuel compositions containing one or more fuels and one or more fuel additives. The methods can advantageously be optimized using combinatorial chemistry, in which a database of combinations of fuel compositions are generated. As market conditions vary and/or product requirements or customer specifications change, conditions suitable for forming desired products can be identified with little or no downtime. | 09-02-2010 |
20100227776 | Rapid Genotyping of SNPs - Embodiments include a universal and generic method for rapidly genotyping essentially any single nucleotide polymorphism (SNP) and or other polymorphism. Various embodiments include procedures for SNP and/or allele analysis that are easy, cheap, highly multiplexable, easily automatable, and lend themselves to high-throughput. Embodiments are broadly applicable for all applications where SNP determinations are useful. | 09-09-2010 |
20100323917 | TAILORED NANOPOST ARRAYS (NAPA) FOR LASER DESORPTION IONIZATION IN MASS SPECTROMETRY - The production and use of semiconducting nanopost arrays made by nanofabrication is described herein. These nanopost arrays (NAPA) provide improved laser ionization yields and controllable fragmentation with switching or modulation capabilities for mass spectrometric detection and identification of samples deposited on them. | 12-23-2010 |
20100331212 | METHODS AND SYSTEMS FOR MONITORING MULTIPLE OPTICAL SIGNALS FROM A SINGLE SOURCE - Methods and systems are described for determining information about template sequences by simultaneously providing excitation over time to a plurality of confined sources on a substrate, each confined source carrying out a template mediated addition of nucleotides with a polymerase enzyme. The nucleotides have different labels, each with a different emission wavelength profile. Optical elements direct optical signals from the different NTPs within each optical confinement to different detector locations. Observing the optical signals over time allows for obtaining sequence information about the template nucleic acids. | 12-30-2010 |
20110028346 | Photonic crystal microarray device for label-free multiple analyte sensing, biosensing and diagnostic assay chips - Methods and systems for label-free multiple analyte sensing, biosensing and diagnostic assay chips consisting of an array of photonic crystal microcavities along a single photonic crystal waveguide are disclosed. The invention comprises an on-chip integrated microarray device that enables detection and identification of multiple species to be performed simultaneously using optical techniques leading to a high throughput device for chemical sensing, biosensing and medical diagnostics. Other embodiments are described and claimed. | 02-03-2011 |
20110039730 | NANOSCALE OPTOFLUIDIC DEVICES FOR MOLECULAR DETECTION - An optofluidic architecture for label free, highly parallel, detection of molecular interactions is based on the use of optically resonant devices whose resonant wavelength is shifted due to a local change in refractive index caused by a positive binding event between a surface bound molecule and its solution phase target. These devices have an extremely low limit of detection and are compatible with aqueous environments. The device combines the sensitivity (limit of detection) of nanosensor technology with the parallelity of the microarray type format. | 02-17-2011 |
20110053798 | SYSTEM FOR MIXING FLUIDS BY COALESCENCE OF MULTIPLE EMULSIONS - System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions. | 03-03-2011 |
20110059864 | Sequence Determination By Use Of Opposing Forces - The present teachings relate to systems, methods, and the like, for analyzing biological polymers, by use of opposing forces. Among other things, the present teachings can be used to determine sequence information, such as in genetic sequencing and genotyping applications. Various embodiments are described for efficient, high throughput sequencing of nucleic-acid molecules, such as DNA. Various embodiments are described wherein nucleic-acid sequence information is determined without the need or use of extrinsic labels. As well various embodiments of methods, systems, and the like, are described, which can provide long and accurate read lengths for low-cost nucleic acid sequencing. | 03-10-2011 |
20110105361 | MICROVESSELS, MICROPARTICLES, AND METHODS OF MANUFACTURING AND USING THE SAME - A method of reading a plurality of encoded microvessels used in an assay for biological or chemical analysis. The method can include providing a plurality of encoded microvessels. The microvessels can include a respective microbody and a reservoir core configured to hold a substance in the reservoir core. The microbody can include a material that surrounds the reservoir core and facilitates detection of a characteristic of the substance within the reservoir core. Optionally, the material can be transparent so as to facilitate detection of an optical characteristic of a substance within the reservoir core. The microbody can include an identifiable code associated with the substance. The method can also include determining the corresponding codes of the microvessels. | 05-05-2011 |
20110111982 | MAGNETIC PARTICLES - A magnetic particle comprises a polysaccharide matrix and a plurality of magnetic crystals dispersed in the matrix. A method for making magnetic particles comprises combining a basic solution with a metal ion solution and allowing the metal ions to oxidize to form magnetic crystals, and combining the magnetic crystals with a polysaccharide solution to form the magnetic particles. | 05-12-2011 |
20110118145 | COPY NUMBER ANALYSIS OF GENETIC LOCUS - Systems and methods for analyzing copy number of a target locus, detecting a disease associated with abnormal copy number of a target gene or a carrier thereof. | 05-19-2011 |
20110136691 | GOLD-SILVER ALLOY NANOPARTICLE CHIP, METHOD OF FABRICATING THE SAME AND METHOD OF DETECTING MICROORGANISMS USING THE SAME - Provided are a gold-silver alloy nanoparticle chip, a method of fabricating the same and a method of detecting microorganisms using the same. The gold-silver alloy nanoparticle chip includes a hydrophilized glass substrate, a self-assembled monolayer formed on the glass substrate, and gold-silver alloy nanoparticles fixed on the self-assembled monolayer. The gold-silver alloy nanoparticle chip having such a structure enables microorganisms in a water purifier and tap water to be readily detected and enables detection efficiency to be enhanced. | 06-09-2011 |
20110136692 | PROTEIN MICROARRAYS FOR MASS SPECTROMETRY AND METHODS OF USE THEREFOR - The present invention relates to the use of mass spectrometry to analyze arrays. The present invention provides methods for characterizing solutions comprising one or more proteins using arrays and mass spectrometry. The arrays of the present invention are coated with porous gold and utilize hydrophobic and hydrophilic self-assembled monolayers. | 06-09-2011 |
20110166040 | COMPOSITIONS FOR USE IN IDENTIFICATION OF STRAINS OF E. COLI O157:H7 - The present invention relates generally to strain typing of | 07-07-2011 |
20110177974 | NEURAL PROTEINS AS BIOMARKERS FOR NERVOUS SYSTEM INJURY AND OTHER NEURAL DISORDERS - The present invention identities biomarkers that are diagnostic of nerve cell injury and/or neuronal disorders. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of nerve injury, the cell(s) involved in the injury, and the subcellular localization of the injury. | 07-21-2011 |
20110195866 | AUTOMATED MULTI-STATION SMALL OBJECT ANALYSIS - The present disclosure provides systems and methods for analyzing a plurality of samples of small objects. In various other embodiments, the system ( | 08-11-2011 |
20110218120 | ORDERED TWO- AND THREE-DIMENSIONAL STRUCTURES OF AMPHIPHILIC MOLECULES - The invention pertains, at least in part, to a method for forming an ordered structure of amphiphilic molecules, such as proteins. The method includes contacting a population of amphiphilic molecules with a interface; compressing said population laterally to an appropriate pressure, such that an ordered structure at the interface is formed. The invention also pertains to the two- and three-dimensional ordered structures that are formed using the planar membrane compression method of the invention. | 09-08-2011 |
20110245106 | Sample Carrier and Method for Achieving Comparable Analytical Results By Aligning Test Substances on a Uniform Plane - A sample carrier such as a multi-well platform with one or more recesses, in each of which a substance to be analyzed is disposed, and an analysis method in which one or more substances, each of which is located in a recess of the sample carrier, are aligned on a uniform plane relative to the surface of the sample carrier before being analyzed. | 10-06-2011 |
20110251104 | COMPOSITIONS AND METHODS FOR DETECTING DEACETYLASE ACTIVITY - A method for the quantitative determination of the activity of enzymes in the Sirtuin family through the quantification of the acetyl-ADPr product that is formed is provided. The method described allows for a wide range of substrates, including peptides, intact proteins, or protein complexes (e.g. nucleosomes) to be used as substrates without the need for additional analytical methods to be developed. | 10-13-2011 |
20110263455 | OPTICAL SYSTEM FOR MULTIPLE REACTIONS - The invention relates to optical systems and methods for measurement of samples in multiple sample vessels using an array of light sources where the light from the light sources is divided into multiple light beams, and each beam is directed to a sample vessel. | 10-27-2011 |
20110269643 | MASS SPECTROMETRIC METHODS FOR DETECTING MUTATIONS IN A TARGET NUCLEIC ACID - Fast and highly accurate mass spectrometry-based processes for detecting particular nucleic acid molecules and mutations in the molecules are provided. | 11-03-2011 |
20110269644 | STRUCTURES FOR ENHANCED DETECTION OF FLUORESCENCE - Substrates are provided for use in the detection, identification and analysis of biologic or chemical samples that are labeled with a fluorescent label, in which the plane of maximum fluorescence is displaced from a reflective substrate surface so that the intensity maximum of the standing wave interference pattern of incident and reflected probe radiation is enhanced. The format of the substrates includes substantially planar surfaces as well as substrates with introduced variations to the substrate surface, e.g., depressions, wells, pedestals and the like, disposed in arrays or other similar structures such that one or more fluorophore-comprising objects can be attached thereto. | 11-03-2011 |
20110287975 | Methods and Compositions for Correlating Genetic Markers with Conversion of Medium Chain Polyunsaturated Fatty Acids to Long Chain Polyunsaturated Fatty Acids - The present invention provides methods of identifying a subject having an increased or decreased ability to convert medium chain-polyunsaturated fatty acids (MC-PUFAs) to long chain polyunsaturated fatty acids (LC-PUFAs), comprising: (a) correlating the presence of one or more than one genetic marker in chromosome 11ql2-13, between build 37.1 position 61548559 and build 37.1 position 61560261, with an increased ability to convert MC-PUFAs to LC-PUFAs; and b) detecting the one or more than one genetic marker of step (a) in the subject, thereby identifying the subject as having an increased ability to convert MC-PUFAs to LC-PUFAs. Also provided are methods of correlating one or more genetic markers with an ability to convert MC-PUFAs to LC-PUFAs. | 11-24-2011 |
20110287976 | MICROFLUIDIC SOLUTION FOR HIGH-THROUGHPUT, DROPLET-BASED SINGLE MOLECULE ANALYSIS WITH LOW REAGENT CONSUMPTION - A microfluidic device for a confocal fluorescence detection system has an input channel defined by a body of the microfluidic device, a sample concentration section defined by the body of the microfluidic device and in fluid connection with the input channel, a mixing section defined by the body of the microfluidic device and in fluid connection with the concentration section, and a detection region that is at least partially transparent to illumination light of the confocal fluorescence detection system and at least partially transparent to fluorescent light when emitted from a sample under observation as the sample flows through the detection region. | 11-24-2011 |
20110294699 | METHODS AND COMPOSITIONS FOR UNIVERSAL SIZE-SPECIFIC PCR - Provided herein are products and processes for the amplification, detection and sequencing of short-stranded nucleic acid in the presence of a high background of long-stranded genomic material (e.g., host or maternal nucleic acids). The methods rely on the use of inside and outside primers introduced at varying concentrations, as well as universal amplification reactions that preferentially amplify short, low copy number nucleic acid. | 12-01-2011 |
20110294700 | HIGH-THROUGHPUT QUANTITATION OF CROP SEED PROTEINS - The invention provides a high-throughput method for quantitating plant seed proteins, e.g. seed allergens. Such method involves obtaining a protein sample from the seed; analyzing the sample using mass spectrometry with a set of pre-designed internal standard peptides that are specific to the plant allergens; and monitoring frequencies and intensities of resulting spectra to obtain relative and absolute allergen contents in the seed. The invention also provides a system for high-throughput profiling of plant seed allergens. Such system comprises a set of pre-designed internal standard peptides that are specific to the plant seed allergens and one or more mass spectrometers. | 12-01-2011 |
20110301061 | HIGH THROUGHPUT EXPERIMENTATION METHODS FOR PHASE SEPARATION - A process for testing the effectiveness of demulsifying additives on oil/water emulsions includes adding samples containing differing combinations of oil, water and demulsifier to a plurality of elongate reactor vials, wherein each the elongate reactor vial has a longitudinal axis extending from its bottom to its rim. The plurality of reactor vials are placed into a reaction block mounted on a platform of a shaker, wherein the reactor vials are received in stations of the reaction block in a vertical orientation such that the longitudinal axis of each reactor vial is perpendicular to the platform. The reaction block is pivoted the so that the longitudinal axis of each reactor vial is parallel with the platform in a horizontal orientation. The method further includes agitating the reactor vials with the shaker to simultaneously form an oil/water emulsion in each reactor vial while the reactor vials are in the horizontal orientation and then pivoting the reaction block to return the reactor vials to a vertical orientation. The demulsification of the oil/water emulsion in the plurality of reactor vials is observed with the reactor vials in the vertical orientation. In one embodiment, the method further includes using an imaging device to record the demulsification of the oil/water emulsion. | 12-08-2011 |
20110301062 | RELIABLE FLUORESCENCE CORRECTION METHOD FOR TWO-COLOR MEASUREMENT FLUORESCENCE SYSTEM - Methods and devices use in two-color measurement systems. The methods and devices include methods of making corrections, methods of calculating correction factors, fluorescence scanners, and microarray chips. The said methods and devices enable a user to correct fluorescence intensities for errors caused by the occurrence of FRET and /or cross-talk when two fluorophores are used in two-color fluorescence arrays. | 12-08-2011 |
20110301063 | MULTIPLEXING DERIVATIZED ANAYLTES USING MASS SPECTROSCOPY - This document relates to methods and materials involved in simultaneously determining (i.e., multiplexing) the levels of an analyte in biological samples from multiple subjects using high pressure liquid chromatography-tandem mass spectrometry (LC-MS/MS). For example, methods and materials for derivatizing vitamin D metabolites in samples obtained from multiple subjects (e.g., humans), and combining samples for simultaneous analysis in a single assay are provided. | 12-08-2011 |
20110312534 | METHOD FOR PREDICTION OF HUMAN IRIS COLOR - A method for predicting the iris color of a human, the method comprising:
| 12-22-2011 |
20110312535 | Cartridge And Device For Analyzing Biological Samples Using Temperature-Controlled Biological Reactions - The cartridge according to the invention for analysing biological samples comprises:
| 12-22-2011 |
20110312536 | Flow cytometry for high throughput screening - The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus. | 12-22-2011 |
20110319295 | MASS SPECTROMETRY ASSAY FOR eIF4E AND eIF4E REGULON ACTIVITY - Provided is a highly sensitive high throughput mass spectrometry-based quantitative assay for 4E/4E regulon pathway proteins has been developed which provides for single sample multiplexed analysis, as well as the analysis of protein phosphorylation states. It may be adapted for use as the first single sample analytical method of the 4E/4E regulon biological pathway. | 12-29-2011 |
20120004138 | ELECTROPHORESIS APPARATUS FOR SIMULTANEOUS LOADING OF MULTIPLE SAMPLES - The present invention includes apparatus for simultaneous loading of multiple samples for molecular separation, including a separation area with walls wherein at least one of the walls has apertures having loading sites, a gel located within the separation area, and a plurality of wells within the gel. The apertures are connected to the plurality of wells by channels structurally configured to convey samples from the apertures to the wells. | 01-05-2012 |
20120010105 | LABEL-FREE HIGH THROUGHPUT BIOMOLECULAR SCREENING SYSTEM AND METHOD - A screening system and method are described herein which provide a unique and practical solution for enabling label-free high throughput screening (HTS) to aid in the discovery of new drugs. In one embodiment, the screening system enables direct binding assays to be performed in which a biomolecular interaction of a chemical compound (drug candidate) with a biomolecule (therapeutic target) can be detected using assay volumes and concentrations that are compatible with the current practices of HTS in the pharmaceutical industry. The screening system also enables the detection of bio-chemical interactions that occur in the wells of a microplate which incorporates biosensors and surface chemistry to immobilize the therapeutic target at the surface of the biosensors. The screening system also includes fluid handling and plate handling devices to help perform automated HTS assays. | 01-12-2012 |
20120015847 | DIAGNOSIS OR PROGNOSIS OF LUNG CANCER AND COLORECTAL CANCER BASED ON EXPRESSION LEVEL OF GLUTAREDOXIN 3 - The present invention relates to a biomarker for diagnosing lung cancer and colorectal cancer containing glutaredoxin 3 as an active ingredient, and a kit for diagnosing lung cancer and colorectal cancer using the same biomarker. Glutaredoxin 3 of the present invention is over-expressed in lung cancer tissue and colorectal cancer tissue, which enables early prediction of diagnosis or prognosis of lung cancer and colorectal cancer. Thus, glutaredoxin 3 can be helpfully used as a biomarker for diagnosing lung cancer and colorectal cancer. | 01-19-2012 |
20120028838 | Quantification of Gene Expression - The present invention relates to a method for measuring the amount of a target nucleic acid in a sample using a standard which is designed to have one base difference compared with the gene of interest or a “target nucleic acid sequence.” Use of such standard in combination with a method of “enhancing” the difference in the standard and the test nucleic acid sample using, for example, a base extension reaction carried right at the mutation site allowing amplification of the standard and target nucleic acids with the same efficiency and facilitating quantification of the target nucleic acid. Thereafter a means of quantifying the “enhanced” standard and target nucleic acid samples is used to determine the amount of the target nucleic acid. In the preferred embodiment, the quantification means is Mass Spectrometry. | 02-02-2012 |
20120035080 | OPTICAL LENS SYSTEM AND METHOD FOR MICROFLUIDIC DEVICES - An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber. | 02-09-2012 |
20120046200 | Screening Methods Involving the Detection of Short-Lived Proteins - A method is provided for screening for agents that affect protein degradation rates, the method comprising: taking a library of cells, the cells expressing a fusion protein comprising a reporter protein and a protein encoded by a sequence from a cDNA library derived from a sample of cells, the sequence from the cDNA library varying within the cell library; contacting the library of cells with a plurality of agents which may affect protein degradation rates; for each agent, selecting cells in the library which express short-lived proteins based on whether the cells have different reporter signal intensities than other cells in the library, the difference being indicative of the selected cells expressing shorter lived fusion proteins than the fusion proteins expressed by the other cells in the library; and characterizing the fusion proteins expressed by the selected cells for each agent. | 02-23-2012 |
20120046201 | METHOD FOR CHARACTERIZING AND/OR DETERMINING SAMPLES - Characterizing and/or determining a sample employs an array of at least two of different interacting surfaces, at least one of which comprises a non-specific interacting material non-specifically interacting said sample, at least one labelling reactant, and/or combination of the sample and at least one labelling reactant. The sample and at least one labelling reactant is introduced to interact with said interacting surfaces of said array, wherein said labelling reactant is adapted to change at least one electromagnetically readable property of at least one interacting surface of said array. Then at a predetermined time point said electromagnetically readable property of at least one of said at least two different interacting surfaces of said array is detected to obtain a fingerprint of said sample; and the sample is characterized and/or determined by comparing said fingerprint of said sample with i) at least one fingerprint of at least one corresponding sample, ii) at least one finger print of an array obtained without a sample, and/or iii) at least one fingerprint of known samples. | 02-23-2012 |
20120065104 | COMPOSITIONS AND METHODS FOR A MEMBRANE PROTEIN CRYSTALLIZATION SCREENING KIT - The present invention comprises compositions and methods useful as a system for efficiently determining conditions that result in the formation of crystals of membrane proteins from solutions containing a membrane protein in a purified and soluble state. The system is comprised of two primary components, a solubility screen and a crystallization screen. Each component is a set of solutions. The present invention further provides a kit comprising solutions of the invention and an instructional material for the use thereof. | 03-15-2012 |
20120071352 | Non-labeled virus detection substrate, system, and method based on inverted multi-angular cavity arrays - The present invention discloses a non-labeled virus detection substrate, system, and method based on inverted multi-angular cavity arrays. The virus detection substrate is used together with a Raman spectrometer for virus detection. The virus detection substrate has a metal layer disposed thereon, and inverted multi-angular cavities are formed in the metal layer. The cavities are arranged in a microarray. In order to detect the target, the size of the cavities should be adjusted first. Then, a laser with an optimized wavelength is applied to induce the effect of the surface enhanced Raman scattering. | 03-22-2012 |
20120071353 | APPARATUS AND METHOD FOR PERFORMING NUCLEIC ACID ANALYSIS - The present invention relates to optical confinements, methods of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis. | 03-22-2012 |
20120077707 | MICROWELLS WITH MRI READABLE INDICIA - A microwell including a plurality of MRI-readable indicia. The indicia indicate the identification or location of at least one predefined well or a plurality of wells. The indicia are selected from a mark, figure, number, text, code, barcode, readable texture or indication thereof. | 03-29-2012 |
20120077708 | BETA-2 MICROGLOBULIN AS A BIOMARKER FOR PERIPHERAL ARTERY DISEASE - The present invention provides β2 microglobulin as a biomarker for qualifying or assessing peripheral artery disease in a subject. | 03-29-2012 |
20120077709 | Biomarkers for Differentiating Between Type 1 and Type 2 Diabetes - Biomarkers that are diagnostic of type 1 diabetes, type 2 diabetes and/or diabetic disorder are identified. Detection of different biomarkers of the invention are also diagnostic of the degree of severity of type 1 diabetes, type 2 diabetes and/or diabetic disorder. An analysis includes the parameters of matching for BMI and Tanner stage. Receiver-operator characteristic (ROC) curves were established to assess association of the biomarkers with a disease. | 03-29-2012 |
20120088691 | HIGHLY MULTIPLEXED REAL-TIME PCR USING ENCODED MICROBEADS - Multiple probes/primers expand the capability of single-probe real-time PCR. Multiplex real-time PCR uses multiple probe-based assays, in which each assay have a specific probe labeled with a unique fluorescent dye, resulting in different observed colors for each assay. Real-time PCR instruments can discriminate between the fluorescence generated from different dyes. Different probes/primers are labeled with different dyes that each have unique emission spectra. By combining the encoded microbeads and real-time PCR amplification, it is possible to increase the multiplexity of PCR experiments to a very large number, such as 128 with 7 digit or 4,096 with 12-digit barcode. Oligonucleotide probes/primers labeled with encoded microbeads offer the ability to monitor the reaction kinetics of each probe which is tagged with barcoded beads. | 04-12-2012 |
20120088692 | SYSTEMS AND METHODS FOR CHARACTERIZATION OF MOLECULES - The present invention generally provides systems and methods for the detection, identification, or characterization of differences between properties or behavior of corresponding species in two or more mixtures comprised of molecules, including biomolecules and/or molecules able to interact with biomolecules, using techniques such as partitioning. The experimental conditions established as distinguishing between the mixtures of the molecules using the systems and methods of the invention can also be used, in some cases, for further fractionation and/or characterization of the biomolecules and/or other molecules, using techniques such as single-step or multiple-step extraction, and/or by liquid-liquid partition chromatography. The methods could also be used for discovering and identifying markers associated with specific diagnostics, and can be used for screening for such markers once discovered and identified during diagnostics screening. | 04-12-2012 |
20120094867 | High-Throughput Molecular Rotor Viscometry Assay - Described are compositions and methods related to determining the rate of viscosity change in a suspension in real time. The compositions and methods have a broad range of applications, including the measurement of amylase-mediated liquefaction of a starch suspension. | 04-19-2012 |
20120094868 | DETECTION OF CIRCULATING TUMOR CELLS IN PERIPHERAL BLOOD WITH AN AUTOMATED SCANNING FLUORESCENCE MICROSCOPE - An automated, highly sensitive, specific and potentially quantitative detection method using an automated microscope for identifying and enumerating rare cancer cells in blood and other fluids. | 04-19-2012 |
20120101007 | SILVER NANOPLATES - A sensor for detecting of an analyte in a solution phase comprises a plurality of functionalised silver nanoplates wherein a functionalising agent is directly bonded to the surfaces of the nanoplates. The nanoplates provide a detectable wavelength shift change in their local surface plasmon resonance spectrum in response to the binding of an analyte. Two or more of the nanoplates may be electromagnetically coupled. | 04-26-2012 |
20120108465 | FIBER ARRAY SENSOR - A sensor array is provided including a plurality of fibers being woven to form 3-D periodic fiber structures. A selective number of the fibers include gaseous sensing materials to detect selective gases. A plurality of spacing elements provides adequate spacing between successively arranged nano-fibers. The nano-fibers and spacing elements are arranged to form a 3-D scaffolding structure for detecting specific or combinations of gaseous analytes. | 05-03-2012 |
20120115755 | MICROFLUIDIC MAGNETOPHORETIC DEVICE AND METHODS FOR USING THE SAME - A microfluidic device may employ one or more sorting stations for separating target species from other species in a sample. The separation is driven by magnetophoresis. A sorting station generally includes separate buffer and sample streams. A magnetic field gradient applied to the sorting station deflects the flow path of magnetic particles (which selectively label the target species) from a sample stream into a buffer stream. The buffer stream leaving the sorting station is used to detect or further process purified target species labeled with the magnetic particles. | 05-10-2012 |
20120122731 | Screening molecular libraries using microfluidic devices - Screening in a microfluidic device is mediated by a magnetic field that in some manner displaces or otherwise activates the entities of interest. Entities of interest can be identified and/or separated from one or more other components provided to the microfluidic device. Microfluidic devices may have mechanisms that apply a defined magnetic field to a region of the microfluidic device where library members pass through sequentially and/or in parallel. | 05-17-2012 |
20120172255 | ES-MS OF GLYCOPEPTIDES FOR ANALYSIS OF GLYCOSYLATION - Herein is reported a method for the determination of the glycosylation of an immunoglobulin with electrospray mass spectrometry but without the need for a chromatographic purification step after the digestion of the immunoglobulin and prior to the mass spectrometric analysis. | 07-05-2012 |
20120172256 | CANTILEVERED PROBES HAVING PIEZOELECTRIC LAYER, TREATED SECTION, AND RESISTIVE HEATER, AND METHOD OF USE FOR CHEMICAL DETECTION - The invention provides a liquid cell for an atomic force microscope. The liquid cell includes a liquid cell housing with an internal cavity to contain a fluid, a plurality of conductive feedthroughs traversing the liquid cell housing between the internal cavity and a dry side of the liquid cell, a cantilevered probe coupled to the liquid cell housing, and a piezoelectric drive element disposed on the cantilevered probe. The cantilevered probe is actuated when a drive voltage is applied to the piezoelectric drive element through at least one of the conductive feedthroughs. A method of imaging an object in a liquid medium and a method of sensing a target species with the liquid cell are also disclosed. | 07-05-2012 |
20120184460 | HIGHLY EFFICIENT GENE-REGULATORY ELEMENT SCREENING ASSAY AND COMPOSITIONS FOR PERFORMING THE SAME - Methods of evaluating a gene-regulatory element, including libraries of known or candidate gene-regulatory elements, are provided. Aspects of the methods include cytometrically analyzing a cell comprising a gene-regulatory element construct, e.g., a plasmid, having an activity reporter comprising a first signal reporter domain which produces a first signal operatively coupled to a gene-regulatory element of interest; and a noise reporter comprising a second signal reporter which produces a second signal that is distinguishable from the first signal to obtain the first and second signals. The first signal is then normalized with the second signal to obtain a normalized activity signal, which normalized activity signal is then employed to evaluate the gene-regulatory element. Also provided are reagents, systems and kits that find use in practicing methods of the invention. | 07-19-2012 |
20120190584 | Chip assay having improved efficiency - The chip includes electrodes on a substrate. The electrodes include a working electrode, a reference electrode, and a counter electrode. The reference electrode is constructed so as to not have an intrinsic potential. A self-assembly monolayer is positioned on the reference electrode. The self-assembly monolayer includes spacers and active probes. The active probes are configured to have a higher affinity for a capture probe than the spacers have for the capture probe. | 07-26-2012 |
20120196770 | GEL-ENCAPSULATED MICROCOLONY SCREENING - Provided herein are methods and compositions useful for detecting the production of industrially useful compounds (e.g., isoprenoids, polyketides, and fatty acids) in a cell, for example, a microbial cell genetically modified to produce one or more such compounds. In some embodiments, the methods comprise encapsulating the cell in a hydrogel particle, and detecting the compound within the hydrogel particle. | 08-02-2012 |
20120202708 | COMPLEMENT FACTOR H COPY NUMBER VARIANTS FOUND IN THE RCA LOCUS - Provided herein is a variant in the RCA locus and methods for detecting the presence, absence or amount of multiple forms of the variant. | 08-09-2012 |
20120202709 | Devices and Methods for Producing and Analyzing Microarrays - Devices and methods for producing and analyzing microarrays are disclosed. In an embodiment, a method for converting a library of beads to an array of analytes includes positioning a plurality of beads having one or more analytes bound therein on a solid support in a spatially separated manner, causing the analytes to be released from the plurality of microparticles, and localizing the released analytes in discrete spots. | 08-09-2012 |
20120208722 | SURFACE ENHANCED RAMAN SPECTROSCOPY PLATFORMS AND METHODS - Surface enhanced Raman spectroscopy (SERS) platforms and methods of making and using such platforms are disclosed herein. Such platforms can be made by immobilizing a biomaterial (e.g., a carbohydrate such as a glycan) by reacting an azide-functional group attached to a surface of a solid substrate with at least one cyclooctyne (e.g., a dibenzocyclooctyne) having a biomaterial or biomaterial binding group attached thereto. In certain embodiments the immobilized biomaterial can be detected using, for example, surface enhanced Raman spectroscopy. | 08-16-2012 |
20120220491 | RETENTATE CHROMATOGRAPHY AND PROTEIN CHIP ARRAYS WITH APPLICATIONS IN BIOLOGY AND MEDICINE - Analytes in a sample are resolved by retentate chromatography in a procedure involving adsorbing the analytes on a substrate under a plurality of different selectivity conditions, and detecting the analytes retained on the substrate by desorption spectrometry. The methods are useful in biology and medicine, including clinical diagnostics and drug discovery. | 08-30-2012 |
20120225798 | METHOD FOR NON-INVASIVE PRENATAL DIAGNOSIS - The present invention is directed to methods of detecting nucleic acids in a biological sample. The method is based on a novel combination of a base extension reaction, which provides excellent analytical specificity, and a mass spectrometric analysis, which provides excellent specificity. The method can be used, for example, for diagnostic, prognostic and treatment purposes. The method allows accurate detection of nucleic acids that are present in very small amounts in a biological sample. For example, the method of the present invention is preferably used to detect fetal nucleic acid in a maternal blood sample; circulating tumor-specific nucleic acids in a blood, urine or stool sample; and donor-specific nucleic acids in transplant recipients. In another embodiment, one can detect viral, bacterial, fungal, or other foreign nucleic acids in a biological sample. | 09-06-2012 |
20120231973 | METHOD AND APPARATUS PROVIDING ANALYTICAL DEVICE AND OPERATING METHOD BASED ON SOLID STATE IMAGE SENSOR - An analytical system-on-a-chip can be used as an analytical imaging device, for example, for detecting the presence of a chemical compound. A layer of analytical material is formed on a transparent layer overlying a solid state image sensor. The analytical material can react in known ways with at least one reactant to block light or to allow light to pass through to the array. The underlying sensor array, in turn, can process the presence, absence or amount of light into a digitized signal output. The system-on-a-chip may also include software that can detect and analyze the output signals of the device. | 09-13-2012 |
20120238471 | Ultrasensitive Biochemical Sensing Device and Method of Sensing Analytes - Systems and methods biochemically sense a concentration of a ligand using a sensor having a substrate having a metallic nanoparticle array formed onto a surface of the substrate. A light source is incident on the surface. A matrix is deposited over the nanoparticle array and contains a protein adapted to binding the ligand. A detector detects s-polarized and p-polarized light from the reflective surface. Spacing of nanoparticles in the array and wavelength of light are selected such that plasmon resonance occurs with an isotropic point such that −s and −p polarizations of the incident light result in substantially identical surface Plasmon resonance, wherein binding of the ligand to the protein shifts the resonance such that differences between the −S and −P polarizations give in a signal indicative of presence of the ligand. | 09-20-2012 |
20120238472 | BIOANALYTICAL INSTRUMENTATION USING A LIGHT SOURCE SUBSYSTEM - The invention relates to a light source for irradiating molecules present in a detection volume with one or more selected wavelengths of light and directing the fluorescence, absorbance, transmittance, scattering onto one or more detectors. Molecular interactions with the light allow for the identification and quantitation of participating chemical moieties in reactions utilizing physical or chemical tags, most typically fluorescent and chromophore labels. The invention can also use the light source to separately and simultaneously irradiate a plurality of capillaries or other flow confining structures with one or more selected wavelengths of light and separately and simultaneously detect fluorescence produced within the capillaries or other flow confining structures. In various embodiments, the flow confining structures can allow separation or transportation of molecules and include capillary, micro bore and milli bore flow systems. The capillaries are used to separate molecules that are chemically tagged with appropriate fluorescent or chromophore groups. | 09-20-2012 |
20120277119 | PROCESSES AND COMPOSITIONS FOR METHYLATION-BASED ENRICHMENT OF FETAL NUCLEIC ACID FROM A MATERNAL SAMPLE USEFUL FOR NON-INVASIVE PRENATAL DIAGNOSES - Provided are compositions and processes that utilize genomic regions differentially methylated between a mother and her fetus to separate, isolate or enrich fetal nucleic acid from a maternal sample. The compositions and processes described herein are useful for non-invasive prenatal diagnostics, including the detection of chromosomal aneuplodies. | 11-01-2012 |
20120283137 | MASS LABELS - A reactive mass label for labelling a biological molecule for detection by mass spectrometry, which label comprises a reactive functionality for labelling thiol groups or carbonyl groups. Also provided is a reactive mass label for labelling a biological molecule for detection by mass spectrometry, wherein the mass label comprises the following structure: | 11-08-2012 |
20120329676 | HIGH THROUGHPUT SCREENING METHOD OF ACID-PRODUCING MICROORGANISM - A high throughput screening system and method of an acid-producing microorganism using a mixture of at least two pH indicators are provided. The method may be useful in determining a production amount of an acid, which is a final metabolite secreted by the microorganism, more accurately, rapidly and easily. | 12-27-2012 |
20130012414 | NANOSTRUCTURE BASED METHODS FOR DETECTION STRUCTURE DETERMINATION SEPARATION TRANSPORT EXTRACTION AND CONTROL OF CHEMICAL AND BIOCHEMICAL MATERIAL - The invention describes methods that use nanostructures and quantum confinement to detect and manipulate chemical and biochemical molecules. To increase selectivity and sensitivity nanostructures are built to have the density of states similar to that in analyte that will be detected and operated. Using device that incorporates such nanostructures, measuring electrical and optical properties of nanostructures and analyte or charge and energy transfer between the nanostructures and analyte a rapid and sensitive continuous detection in fluids can be achieved. Detection can be further enhanced by controlling external environmental parameters and applying external fields. In addition to be detected analyte can be positioned, moved, separated, extracted, and controlled. | 01-10-2013 |
20130023444 | COLOR-PRODUCING DIAGNOSTIC SYSTEMS, REAGENTS AND METHODS - Provided herein are novel reagents and their use in color-producing detection systems for performing diagnostic tests and analytical assays. | 01-24-2013 |
20130040857 | MASS SPECTROMETRIC ASSAYS FOR PEPTIDES - Methods for interpretation of mass spectrometric tests for clinical biomarkers in which the amounts of internal standards are set to equal clinical evaluation thresholds, and preparations for adding stable isotope labeled peptide species to sample digests while minimizing losses and alterations in peptide stoichiometry. | 02-14-2013 |
20130059758 | Detection, Staging and Grading of Benign and Malignant Tumors - The present invention provides a method for detecting and grading benign and malignant tumors using at least one sensor of conductive nanoparticles capped with an organic coating in conjunction with a learning and pattern recognition algorithm. The method utilizes a plurality of response induced parameters to obtain improved sensitivity and selectivity for diagnosis, prognosis, monitoring and staging various types of cancers, or for identifying or grading benign or malignant tumors. | 03-07-2013 |
20130079247 | METHOD AND APPARATUS FOR DELIVERY OF SUBMICROLITER VOLUMES ONTO A SUBSTRATE - A slotted pin tool, a delivery system containing the pin tool, a substrate for use in the system and methods using the pin tool and system are provided. The slotted pin tool contains a plurality of pins having slotted ends designed to fit around each loci of material deposited on a surface, such as a microarray, without contacting any of the deposited material. Sample is delivered by contacting the pin tool with the surface; the amount delivered is proportional to the velocity of the pin tool as it contacts the surface or the velocity of the liquid when movement of the pin is halted. | 03-28-2013 |
20130096030 | MULTISENSOR ARRAY FOR DETECTION OF ANALYTES OR MIXTURES THEREOF IN GAS OR LIQUID PHASE - The present invention relates to a multisensor array for detection of analytes in the gas phase or in the liquid phase, comprising at least two different chemo-selective compounds represented by the general formula (I) wherein the hetero atoms X | 04-18-2013 |
20130109592 | METHODS FOR DETECTING CANCER | 05-02-2013 |
20130123144 | TUNABLE LCST POLYMERS AND METHODS OF PREPARATION - Polymer compositions having the chemical structure: as well as monomer compositions for producing said polymers are described. Methods for preparing these polymers and combinatorial libraries of these polymers are also described. | 05-16-2013 |
20130130936 | Method of Conducting an Assay - The invention is directed to droplet actuator devices and assay methods. The invention includes assay methods of conducting an assay comprising combining a sample with an umbelliferyl derivative, wherein the sample potentially comprises an enzyme capable of cleaving the umbelliferyl derivative and where the umbelliferyl derivative comprises an umbelliferyl core modified with one or more modifying moieties. | 05-23-2013 |
20130143768 | SYSTEM AND METHOD FOR GENERATING AND/OR SCREENING POTENTIAL METAL-ORGANIC FRAMEWORKS - A system and method for systematically generating potential metal-organic framework (MOFs) structures given an input library of building blocks is provided herein. One or more material properties of the potential MOFs are evaluated using computational simulations. A range of material properties (surface area, pore volume, pore size distribution, powder x-ray diffraction pattern, methane adsorption capability, and the like) can be estimated, and in doing so, illuminate unidentified structure-property relationships that may only have been recognized by taking a global view of MOF structures. In addition to identifying structure-property relationships, this systematic approach to identify the MOFs of interest is used to identify one or more MOFs that may be useful for high pressure methane storage. | 06-06-2013 |
20130150261 | BREATH ANALYSIS OF PULMONARY NODULES - The present invention provides a unique profile of volatile organic compounds as breath biomarkers for lung cancer. The present invention further provides the diagnosis, prognosis and monitoring of lung cancer or predicting the response to an anti-cancer treatment through the detection of the unique profile of volatile organic compounds indicative of lung cancer at its various stages. | 06-13-2013 |
20130157896 | ULTRATHIN CALCINATED FILMS ON A GOLD SURFACE FOR HIGHLY EFFECTIVE LASER DESORPTION/ IONIZATION OF BIOMOLECULES - A nanoscale calcinated silicate film fabricated on a gold substrate for highly effective, matrix-free laser desorption ionization mass spectrometry (LDI-MS) analysis of biomolecules. The calcinated film is prepared by a layer-by-layer (LbL) deposition/calcination process wherein the thickness of the silicate layer and its surface properties are precisely controlled. The film exhibits outstanding efficiency in LDI-MS with extremely low background noise in the low-mass region, allowing for effective analysis of low mass weight samples and detection of large biomolecules including amino acids, peptides and proteins. Additional advantages for the calcinated film include ease of preparation and modification, high reproducibility, low cost and excellent reusability. | 06-20-2013 |
20130165346 | SYSTEM AND METHOD FOR SCREENING A LIBRARY OF SAMPLES - A continuous throughput microfluidic system includes an input system configured to provide a sequential stream of sample plugs; a droplet generator arranged in fluid connection with the input system to receive the sequential stream of sample plugs and configured to provide an output stream of droplets; a droplet treatment system arranged in fluid connection with the droplet generator to receive the output stream of droplets in a sequential order and configured to provide a stream of treated droplets in the sequential order; a detection system arranged to obtain detection signals from the treated droplets in the sequential order; a control system configured to communicate with the input system, the droplet generator, and the droplet treatment system; and a data processing and storage system configured to communicate with the control system and the detection system. | 06-27-2013 |
20130178395 | Aza-Benzazolium Containing Cyanine Dyes - Unsymmetrical cyanine dyes that incorporate an aza-benzazolium ring moiety are described, including cyanine dyes substituted by a cationic side chain, monomeric and dimeric cyanine dyes, chemically reactive cyanine dyes, and conjugates of cyanine dyes. The subject dyes are virtually non-fluorescent when diluted in aqueous solution, but exhibit bright fluorescence when associated with nucleic acid polymers such as DNA or RNA, or when associated with detergent-complexed proteins. A variety of applications are described for detection and quantitation of nucleic acids and detergent-complexed proteins in a variety of samples, including solutions, electrophoretic gels, cells, and microorganisms. | 07-11-2013 |
20130184180 | CHARACTERIZATION OF N-GLYCANS USING EXOGLYCOSIDASES - The present disclosure provides methods for analyzing structure and/or composition of N-glycans. Such methods often involve digestion of N-glycans with multiple exoglycosidases. In some embodiments, N-glycans are digested with multiple exoglycosidases simultaneously. In some embodiments, N-glycans are digested with multiple exoglycosidases sequentially. In some embodiments, methods in accordance with the present disclosure involve comparison of cleavage products of N-glycans that have been digested with multiple exoglycosidases simultaneously to N-glycans that have been digested with multiple exoglycosidases sequentially. | 07-18-2013 |
20130184181 | ANALYTE DETERMINATION UTILIZING MASS TAGGING REAGENTS COMPRISING A NON-ENCODED DETECTABLE LABEL - This invention pertains to methods, mixtures, kits and compositions pertaining to analyte determination and/or quantification by mass spectrometry using compounds comprising a reporter moiety and a non-encoded detectable label. The compounds can be used in sets for the analysis of mixtures of labeled analytes. | 07-18-2013 |
20130210672 | Flow Cytometry For High Throughput Screening - The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus. | 08-15-2013 |
20130217596 | Detection Device And Methods Of Use - An imaging system for exciting and measuring fluorescence on or in samples comprising fluorescent materials (e.g. fluorescent labels, dyes or pigments). In one embodiment, a device is used to detect fluorescent labels on nucleic acid. In a preferred embodiment, the device is configured such that fluorescent labels in a plurality of different DNA templates are simultaneously detected. | 08-22-2013 |
20130237454 | DIAGNOSTIC MARKERS FOR NEUROPSYCHIATRIC DISEASE - Biomarkers for the diagnosis of neuropsychiatric diseases are presented herein. In particular embodiments, biomarkers are identified that are useful for diagnosing multiple sclerosis, chronic fatigue syndrome, or Neurologic Lyme disease. Also encompassed is a method for diagnosing a patient with a neuropsychiatric disease, such as multiple sclerosis, chronic fatigue syndrome, or Neurologic Lyme disease, by analyzing biological samples isolated from the patient or the patient as a whole to assess levels of the biomarkers described herein. | 09-12-2013 |
20130244906 | Microfluidic devices and methods based on massively parallel picoreactors for cell and molecular diagnostics - Microfluidic devices and methods that use massively parallel anchored picoreactors as the basis for cell and molecular diagnostics (e.g., characterizing, isolation, processing, amplification) different particles, chemical compositions or biospecies (e.g., different cells, cells containing different substances, different particles, different biochemical compositions, proteins, enzymes etc.). | 09-19-2013 |
20130261022 | METABOLOMIC PROFILING OF PROSTATE CANCER - The present invention relates to cancer markers. In particular, the present invention provides metabolites and panels of metabolites that are differentially present in cancer (e.g., prostate or breast cancer). | 10-03-2013 |
20130267441 | NOVEL BIOMARKER AND USES THEREOF IN DIAGNOSIS, TREATMENT OF AUTISM - A new biomarker, a peptide having sequence SSKITHRIHWESASLLR*, wherein the side chain of the C-terminal arginine denoted with the asterisk is lacking the NH2-C═NH moiety normally present in the side chain. Usefulness of the biomarker in the diagnosis of neurological and/or neuropsychiatric disorders (in particular autism) is disclosed, as well as are methods for determining the concentration of the new biomarker and antibodies directed to the new biomarker. Treatment of autism, comprising administering a complement factor I inhibitor to the subject. | 10-10-2013 |
20130274143 | USE OF DETECTOR RESPONSE CURVES TO OPTIMIZE SETTINGS FOR MASS SPECTROMETRY - Processes for identifying optimal mass spectrometer settings to produce the greatest confidence in sample constituent detection are provided. Data obtained on a mass spectrometer are analyzed by a quadratic variance function which accurately represents intensity variation as a variation of peak intensity. This function is then used to identify intensities that possess a minimum coefficient of variation that is useful for identifying optimal mass spectrometer settings. Inventive processes involve using a general purpose computer to identify optimal mass spectrometer settings for use in biomarker analyses, for optimizing peak detection and biomarker identification in a biological sample. The inventive processes provide for improved methods of identifying new biomarkers as well as screening subjects for the presence or absence of disease or biological condition. | 10-17-2013 |
20130274144 | COMPOUNDS, REACTIONS, AND SCREENING METHODS - The invention provides a method comprising identifying a successful metal-mediated conjugation reaction by analyzing a test mixture for the presence of a conjugation product. The invention provides a two-dimensional approach to reaction discovery in which many catalysts for many catalytic reactions can be tested simultaneously to provide an efficient discovery platform. Reactants and products from the system can be identified using techniques such as gas chromatography, liquid chromatography, mass spectrometry, and combinations thereof. | 10-17-2013 |
20130303401 | SYSTEMS AND METHODS FOR DIAGNOSING RENAL CELL CARCINOMA - Systems, methods, and computer readable media for diagnosing or characterizing kidney cancer based on serum amino acid profiles are provided. Serum amino acid concentrations, and optionally also serum creatinine concentration, are determined in serum obtained from a subject and compared against reference concentration profiles. The condition or prognosis of the subject may be determined based on comparisons of patient samples with reference profiles. | 11-14-2013 |
20130303402 | Detection and Molecular Weight Determination of Organic Vapors - Composite films comprising an organic salt (or GUMBOS) such as 1-n-butyl-2,3-dimethylimidazolium hexafluoro-phosphate and a polymer such as cellulose acetate are prepared. These films are useful in detecting vapors of volatile organic compounds, and in determining their molecular weights. A quartz crystal microbalance-based sensor was designed by depositing a thin film of this composite material on the gold electrode surface of a quartz crystal resonator. The sensor exhibited rapid response toward a variety of volatile organic compounds, and complete regeneration, high sensitivity, low detection limits, and wide dynamic ranges. The ratio of the change in frequency to the change in motional resistance is a concentration-independent quantity that is proportional to the molecular weight of the absorbed chemical species. These properties facilitate the easy identification and molecular weight determination of a broad range of organic vapors. | 11-14-2013 |
20130303403 | MICROARRAY ANALYSIS METHOD AND MICROARRAY READING DEVICE - A microarray analysis method, in which a microarray obtained by arranging probes on a substrate surface having an irregular shape is irradiated with excitation light and fluorescence amounts of the probes excited by the excitation light are obtained as numerical data, includes a step (a) of measuring the fluorescence amounts of the probes to acquire fluorescence image data, a step (b) of receiving reflected light and/or scattered light from the substrate surface to acquire the irregular shape of the substrate surface of the microarray as alignment image data based on the light receiving intensities of the light, and a step (c) of determining positions of the probes on the fluorescence image data based on the alignment image data. | 11-14-2013 |
20130316934 | SYSTEMS AND METHODS FOR DETECTION OF PARTICLES IN A BENEFICIAL AGENT - Detection of particles in a liquid beneficial agent contained within a container includes selectively illuminating at least a portion of the liquid beneficial, obtaining an image from the illuminated portion of the liquid beneficial agent, analyzing image data representing the image, using a data processor, to obtain a particle concentration, measuring an image intensity value of the image data using the data processor, and determining a quality level of the liquid beneficial agent using the data processor based on the particle concentration and the measured image intensity value. | 11-28-2013 |
20130331295 | LIGHT ADDRESSING BIOSENSOR CHIP AND METHOD OF DRIVING THE SAME - Provided is a biosensor chip. The biosensor chip includes a plurality of biosensor cells that are arranged in a matrix and selectively generate and output a sensed signal by addressing of external light, at least one sensing line that is simultaneously connected with the plurality of biosensor cells and transmits the sensed signal from one selected from the biosensor cells, and an output terminal that receives the sensed signal from the sensing line and outputs the sensed signal to an external reader. Thus, the biosensor cells are set in array in the biosensor chip without a separate driving unit, so that a process of manufacturing the biosensor chip is simplified. The biosensor cell to be sensed is selectively addressed through the external light, so that it is possible to reduce a price of the biosensor chip used as a disposable chip. | 12-12-2013 |
20130331296 | Method and System for Combinatorial Electroplating and Characterization - The embodiments describe a system for combinatorial processing of a substrate. In one embodiment, electrodeposition processing techniques are combinatorially evaluated. The system is capable of providing a localized electrical connection to each region of a substrate being combinatorially processed. The localized electrical contacts allow for varying a voltage delivered to each region of a substrate whether processing the regions in serial or parallel. Accordingly, from a single substrate, a variety of materials, process conditions, and process sequences may be evaluated for desired electrodeposition results. | 12-12-2013 |
20130345093 | Method for Detection and Quantification of Target Biomolecules - The invention relates to a method for detection and quantification of target biomolecules, such as DNA, RNA or proteins, using exogenous element labelling and dynamic secondary ion mass spectrometry. | 12-26-2013 |
20140005073 | METHOD AND APPARATUS FOR ANALYZING NUCLEIC ACID BY COMPENSATING FOR CROSSTALK IN POLYMERASE CHAIN REACTION DATA AND OTHER DATA | 01-02-2014 |
20140005074 | METHOD FOR AUTOMATED, LARGE-SCALE MEASUREMENT OF THE MOLECULAR FLUX RATES OF THE PROTEOME OR THE ORGANEOME USING MASS SPECTROMETRY | 01-02-2014 |
20140024559 | Optical Lens System and Method for Microfluidic Devices - An apparatus for imaging one or more selected fluorescence indications from a microfluidic device. The apparatus includes an imaging path coupled to least one chamber in at least one microfluidic device. The imaging path provides for transmission of one or more fluorescent emission signals derived from one or more samples in the at least one chamber of the at least one microfluidic device. The chamber has a chamber size, the chamber size being characterized by an actual spatial dimension normal to the imaging path. The apparatus also includes an optical lens system coupled to the imaging path. The optical lens system is adapted to transmit the one or more fluorescent signals associated with the chamber. | 01-23-2014 |
20140045724 | FORMULATION SCREENING METHODS, APPARATUSES FOR PERFORMING SUCH METHODS AND FORMULATIONS FORMED BY SUCH METHODS - A method of screening for candidate compound-excipient combinations comprises dosing a compound into each receptacle of a collection of receptacles, dosing a first set of excipients and a second set of excipients into the receptacles wherein the dosing creates varying combinations of solutions of a first and second excipients within the receptacles, analyzing each receptacle for the presence of a precipitate, and classifying the receptacles based on the presence of a precipitate. | 02-13-2014 |
20140045725 | METHOD FOR IDENTIFICATION AND PURIFICATION OF MULTI-SPECIFIC POLYPEPTIDES - The document pertains to a method for the purification of a ternary mixture of dimeric antibodies of the type AA, AB, BB, characterised in that for the separation of the three components and in particular for the isolation of the multi-specific fraction AB multicolumn counter current solvent gradient purification chromatography with a stationary phase load of more than 1 mg antibody mixture per millilitre stationary phase is used. It furthermore relates to a method for the identification of in particular bispecific antibody systems, which are particularly suitable for the application of such a purification method. | 02-13-2014 |
20140051601 | METABOLOMIC PROFILING OF CANCER - The present invention relates to cancer markers. In particular, the present invention provides metabolites that are differentially present in prostate cancer. | 02-20-2014 |
20140051602 | DETECTING ANALYTES USING LIPID MULTILAYER GRATINGS WITH ION CHANNELS - A method comprising the following step: determining that one or more analytes are present in a fluid to which an array of lipid multilayer gratings has been exposed based on scattered light detected by a detector, wherein each lipid multilayer grating of the array of lipid multilayer gratings comprises iridescent lipid multilayer nanostructures, wherein the scattered light is formed by scattering one or more incident lights by the array of lipid multilayer gratings, and wherein the one or more lipid multilayer gratings comprise ion channels that are activated by the one or more analytes. | 02-20-2014 |
20140057805 | DNA-ORIGAMI-BASED STANDARD - Arrays which utilize labeling molecules for calibrating a measuring device, such as microscopes, have a first structure based on a DNA origami as a calibration sample, wherein the DNA origami is formed into a predetermined structure by short DNA segments. The DNA origami is optionally present in an arranged manner on a support, wherein a number of short DNA segments which form the predetermined structure include a labeling molecule. Optionally, the array can have at least a second structure based on a DNA origami, different from the first structure, as a calibration sample. The array allows quantification of the labeling molecules on the basis of the number of photons per unit time. | 02-27-2014 |
20140087971 | MULTIPLEXED FLOW ASSAY BASED ON ABSORPTION-ENCODED MICRO BEADS - Analysis of a system and/or sample involves the use of absorption-encoded micro beads. Each type of micro bead is encoded with amounts of the k dyes in a proportional relationship that is different from proportional relationships of the k dyes of others of the n types of absorption-encoded micro beads. A system and/or a sample can be analyzed using information obtained from detecting the one or more types of absorption-encoded micro beads. | 03-27-2014 |
20140100139 | METHOD FOR SCHEDULING SAMPLES IN A COMBINATIONAL CLINICAL ANALYZER - A method for scheduling the order of analysis of multiple samples in a combinational clinical analyzer performing a plurality of different analytical tests, includes the steps of: loading multiple samples in random order into a combinational clinical analyzer; defining the test requirements of the multiple samples; transferring said test requirements to a flexible scheduling algorithm; and generating a schedule specifying the start times of each required test for each of said multiple samples that minimizes or maximizes a predefined objective function. In a preferred embodiment, the objective function is the makespan or weighted makespan. | 04-10-2014 |
20140106988 | Addressable Electrode Arrays in Multiple Fluidic Compartments and Uses Thereof - Methods and apparati for performing measurements of electrical signals in a multi-compartmented fluidic array format where the signal to noise ratio is improved are disclosed. | 04-17-2014 |
20140106989 | IMAGE CAPTURE FOR LARGE ANALYTE ARRAYS - Analyte arrays such as solutes in a slab-shaped gel following electrophoresis, and particularly arrays that are in excess of 3 cm square and up to 25 cm square and higher, are imaged at distances of 5 cm or less by either forming sub-images of the entire array and stitching together the sub-images by computer-based stitching technology, or by using an array of thin-film photoresponsive elements that is coextensive with the analyte array to form a single image of the array. | 04-17-2014 |
20140155293 | IDENTIFICATION OF BIOMARKERS FOR DETECTING PROSTATE CANCER - The present invention relates to a method of qualifying prostate cancer status in a subject comprising: (a) measuring at least one of the disclosed biomarkers in a sample from the subject and (b) correlating the measurement with prostate cancer status. The invention further relates to kits for qualifying prostate cancer status in a subject. | 06-05-2014 |
20140162903 | Metabolite Biomarkers For Forecasting The Outcome of Preoperative Chemotherapy For Breast Cancer Treatment - The present disclosure relates to a panel of metabolite species that is useful for forecasting the outcome of preoperative chemotherapy for the treatment of breast cancer, including methods for identifying and using such metabolite species that can be measured in biological samples taken before treatment. In preferred embodiments, a method of forecasting a treatment outcome before subjecting a breast cancer patient to preoperative chemotherapy is disclosed that includes measuring the concentration of at least one metabolite species in a sample of a biofluid taken from the breast cancer patient before preoperative chemotherapy treatment. | 06-12-2014 |
20140171341 | MULTIPLEXED DIGITAL ASSAYS - System, including methods and apparatus, for performing a multiplexed digital assay. | 06-19-2014 |
20140179559 | COMPUTER-IMPLEMENTED METHOD FOR IDENTIFYING DIFFERENTIALLY EXPRESSED GENES AND COMPUTER READABLE STORAGE MEDIUM FOR STORING THE METHOD - A method for identifying differentially expressed genes including the following steps: measure gene expression levels of test samples and control samples; estimate variances of noise in the test samples and in the control samples; based on the measured expression levels and the estimated variances of noise, derive a probability density function (PDF) for predicting the true value of each expression level measurement; normalize the PDFs; based on the normalized PDFs of the gene under test, derive a test-group PDF for predicting the gene's mean expression level in the test samples and derive a control-group PDF for predicting the gene's mean expression level in the control samples; based on the test-group PDF and the control-group PDF of the gene under test, derive a final PDF for predicting the gene's fold-change; use the final PDF to test whether the gene is differentially expressed. | 06-26-2014 |
20140179560 | DRUG DISCOVERY METHODS AND PLATFORMS - Methods and systems for discovering drug candidates are disclosed. Methods and systems can include generating libraries of potential drug candidates (e.g., libraries of peptides) that can be screened to identify sub-libraries of potential drug candidates (e.g., sub-libraries of peptides) having selected pharmacological properties. Methods of making and using peptide libraries are also provided. D-amino acid chlorotoxins and D-amino acid chlorotoxin variants are also provided. | 06-26-2014 |
20140187442 | FLOW CYTOMETRY FOR HIGH THROUGHPUT SCREENING - The present invention, provides a flow cytometry apparatus for the detection of particles from a plurality of samples comprising: means for moving a plurality of samples comprising particles from a plurality of respective source wells into a fluid flow stream; means for introducing a separation gas between each of the plurality of samples in the fluid flow stream; and means for selectively analyzing each of the plurality of samples for the particles. The present invention also provides a flow cytometry method employing such an apparatus. | 07-03-2014 |
20140187443 | ANTIBACTERIAL AND PLASMID ELIMINATION AGENTS - Inhibitors of the tmRNA pathway have antibacterial activity with broad species specificity, including | 07-03-2014 |
20140200164 | SYSTEM FOR DETECTION OF SPACED DROPLETS - System, including methods and apparatus, for detection of spaced droplets. | 07-17-2014 |
20140221249 | NANOPORE ARRAYS - A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules. | 08-07-2014 |
20140235503 | PREDICTION METHOD OF GLOMERULAR FILTRATION RATE FROM URINE SAMPLES AFTER KIDNEY TRANSPLANTATION - Disclosed is a prediction method of glomerular filtration rate (GFR) from urine samples after kidney transplantation to provide an information needed for predict renal function after the transplantation, more particularly to a prediction method of glomerular filtration rate (GFR) from urine samples after kidney transplantation, which comprises detecting metabolic profiles of five biomarkers, 5a-androst-3-en-17-one (AS), glycocholic acid (GC), sphingosine (SG), tryptophan (TR) and histidine (HT), from urine samples of patients. Glomerular filtration rate (GFR) after kidney transplantation can be predicted more rapidly and precisely to provide an information needed for predict renal function after the transplantation by using five metabolites as biomarkers. The method provides more specific, sensitive, and reliable biomarkers that monitor clinical outcomes and adverse renal events after kidney transplantation, such as rejection, drug toxicity, delayed graft function, and infection. | 08-21-2014 |
20140235504 | ANALYTIC METHOD AND DEVICE WITH A MOVABLE PLUNGER - A method and a device for analyzing a liquid is provided, in which the liquid is distributed to a plurality of containers, and which enables the rapid and inexpensive performing of a multitude of analyses in liquids, in particular DNA analyses, in micro-arrays. The liquid is supplied to a transparent analytical plate which includes a matrix with a plurality of at least unilaterally closed receptacle chambers arranged adjacent to each other. Axially-displaceable plungers are moved towards walls of the receptacle chambers and the analytical plates are transported towards the plungers using a transport apparatus. The liquid can be distributed into the receptacle chambers using mobile elements of the analytical plate, and the elements may be moved by the plungers. At least one axially displaceable plunger may be heated to a preset temperature value and impart this temperature to the accommodating chamber. | 08-21-2014 |
20140249055 | SYSTEMS AND METHODS FOR SELF-REFERENCED DETECTION AND IMAGING OF SAMPLE ARRAYS - A system for detecting an array of samples having detectable samples and at least one reference sample is provided. The system comprises an electromagnetic radiation source, a sensing surface comprising a plurality of sample fields, wherein the plurality of sample fields comprise at least one reference field, a phase difference generator configured to introduce differences in pathlengths of one or more samples in the array of samples, and an imaging spectrometer configured to image one or more samples in the array of samples. | 09-04-2014 |
20140274799 | MULTIPLEXED DIGITAL ASSAY FOR VARIANT AND NORMAL FORMS OF A GENE OF INTEREST - System, including methods, apparatus, and compositions, for performing a multiplexed digital assay of at least three targets. In an exemplary method, partitions may be formed each including a portion of a same sample. The sample may include a first target that is a reference sequence, a second target that is a variant sequence from a gene of interest, and a third target that is a normal sequence from the gene of interest. The target may be amplified in the partitions and amplification data may be collected. A level of the second target may be determined. A copy number of the third target may be determined from a level of the first target and a level of the third target. | 09-18-2014 |
20140287957 | ISOTOPIC LABELING FOR THE MEASUREMENT OF GLOBAL PROTEIN LEVELS AND TURNOVER IN VIVO - An entire complement or plurality of isotopically labeled amino acids are introduced into the diet of a test subject. Sufficient amounts of the isotopically labeled amino acids are provided to the subject in order to ensure that the subject incorporates a large percentage of isotopically labeled amino acids into newly synthesized proteins. Tissue samples are removed from the subject at different points in time and proteins are extracted and separated so that different proteins of different tissues can be individually analyzed and their amount and pattern of isotopic labeling can be determined. In a preferred embodiment, the methodology can be combined with proteolytic digestion to peptides and analysis by mass spectrometry in order to measure rates of protein turnover in vivo relating to thousands of different proteins. | 09-25-2014 |
20140287958 | CANTILEVERED PROBES HAVING PIEZOELECTRIC LAYER, TREATED SECTION, AND RESISTIVE HEATER, AND METHOD OF USE FOR CHEMICAL DETECTION - The invention provides a liquid cell for an atomic force microscope. The liquid cell includes a liquid cell housing with an internal cavity to contain a fluid, a plurality of conductive feedthroughs traversing the liquid cell housing between the internal cavity and a dry side of the liquid cell, a cantilevered probe coupled to the liquid cell housing, and a piezoelectric drive element disposed on the cantilevered probe. The cantilevered probe is actuated when a drive voltage is applied to the piezoelectric drive element through at least one of the conductive feedthroughs. A method of imaging an object in a liquid medium and a method of sensing a target species with the liquid cell are also disclosed. | 09-25-2014 |
20140296108 | BIOMARKERS AND METHODS FOR PREDICTING PREECLAMPSIA - The disclosure provides biomarker panels, methods and kits for determining the probability for preeclampsia in a pregnant female. The present disclosure is based, in part, on the discovery that certain proteins and peptides in biological samples obtained from a pregnant female are differentially expressed in pregnant females that have an increased risk of developing in the future or presently suffering from preeclampsia relative to matched controls. The present disclosure is further based, in part, on the unexepected discovery that panels combining one or more of these proteins and peptides can be utilized in methods of determining the probability for preeclampsia in a pregnant female with relatively high sensitivity and specificity. These proteins and peptides disclosed herein serve as biomarkers for classifying test samples, predicting a probability of preeclampsia, monitoring of progress of preeclampsia in a pregnant female, either individually or in a panel of biomarkers. | 10-02-2014 |
20140309142 | METHOD OF ON-CHIP NUCLEIC ACID MOLECULE SYNTHESIS - A method of synthesizing a nucleic acid molecule, such as a gene, on a substrate or microchip is described. In particular, a method for synthesizing, amplifying, and assembling DNA oligonucleotides into a nucleic acid molecule or gene product, on a single substrate or microchip is described. Also described are a method of correcting a sequence error in a synthesized nucleic acid molecule, as well as a method for synthesizing and screening a library of codon variants to identify a nucleic acid molecule with an optimized level of protein expression. | 10-16-2014 |
20140309143 | CENTROID MARKERS FOR IMAGE ANALYSIS OF HIGH DENSITY CLUSTERS IN COMPLEX POLYNUCLEOTIDE SEQUENCING - Improved compositions, methods, apparatus, and kits for high-throughput nucleic acid amplification, detection and sequencing are disclosed. A nucleic acid cluster having an identifiable center is produced by generating on a solid support an immobilized nucleic acid complement from a template, one of which comprises a detectable label; and amplifying the complement and the template to obtain a nucleic acid cluster on the support, the cluster having a substantially central location marked by the detectable label and a surrounding region comprising immobilized copies. Also disclosed are nucleotide sequence determination in nucleic acid clusters so produced, center position annotation in the clusters, assignment of sequence information to overlapping clusters, and related compositions and methods. | 10-16-2014 |
20140323352 | Means and Methods for Diagnosing Pancreatic Cancer in a Subject - The present invention relates to the field of diagnostic methods. Specifically, the present invention contemplates a method for diagnosing pancreatic cancer in a subject, a method for identifying whether a subject is in need for a therapy of pancreatic cancer or a method for determining whether a pancreatic cancer therapy is successful. The invention also relates to tools for carrying out the aforementioned methods, such as diagnostic devices. | 10-30-2014 |
20140336080 | METHOD OF DIAGNOSING PNEUMONIA BY DETECTING A VOLATILE ORGANIC COMPOUND - The present invention relates to a method of diagnosing an existing or developing acute pneumonia induced by a microorganism by detecting one or more volatile organic compound(s) in a subject's sample and the use of one or more volatile organic compound(s) for the detection of | 11-13-2014 |
20140342945 | FORENSIC METHOD - The present invention relates to a forensic method for identifying an organ penetrated by a corpus delicti. | 11-20-2014 |
20140349885 | ENHANCEMENT OF SENSITIVITY AND SPECIFICITY OF KETOSTEROIDS AND KETO OR ALDEHYDE CONTAINING ANALYTES - A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the relative quantitation, absolute quantitation, or both, of ketone or aldehyde compounds including, but not limited to, analytes comprising steroids or ketosteroids. The analytes can be medical or pharmaceutical compounds in biological matrices. Methods for labeling, analyzing, and quantifying ketone or aldehyde compounds are also disclosed as are methods that also use mass spectrometry. | 11-27-2014 |
20140357525 | MARKERS FOR ALZHEIMER'S DISEASE AND MILD COGNITIVE IMPAIRMENT AND METHODS OF USING THE SAME - Disclosed herein are markers for diagnosing Alzheimer's Disease and/or mild cognitive impairment, for predicting risk of developing Alzheimer's Disease and/or mild cognitive impairment, and for monitoring the efficacy of treatment for Alzheimer's Disease and/or mild cognitive impairment. Also disclosed herein are methods of diagnosing Alzheimer's Disease and/or mild cognitive impairment in a subject in need thereof, for predicting risk of developing Alzheimer's Disease and/or mild cognitive impairment in a subject in need thereof, and for monitoring the efficacy of a treatment for Alzheimer's Disease and/or mild cognitive impairment in the subject. | 12-04-2014 |
20140357526 | HYDROGEL-MEDIATED TISSUE ANALYSIS - A method for analyzing the polypeptide content of animal tissue is described. The method includes the steps of (a) providing an animal tissue specimen; (b) depositing one or more portions of a hydrogel mixture including a protease on spatially discrete portions of the animal tissue specimen; (c) allowing sufficient time to pass for animal tissue under the hydrogel mixture to be form a digested mixture of animal tissue and hydrogel mixture; (d) removing the digested mixture from the animal tissue and extracting the polypeptides from the digested mixture to provide an extract; and (e) analyzing the polypeptide content of the extract by mass spectrometry. | 12-04-2014 |
20140357527 | METHOD AND APPARATUS FOR MEASURING PHOSPHORYLATION KINETICS ON LARGE ARRAYS - The disclosure provides for methods and apparatuses relating to technology for monitoring chemical and/or biological reactions. Some methods provided herein relate to utilization of NAPPA technology to create large protein arrays suitable for use in combination with various ISFET arrays to enable massive parallel assays of kinase activity and inhibition. Some devices provided herein relate to CMOS chips which utilize the NAPPA array technology to build protein inventories of interest upon an ISFET architecture. Further devices provided herein are capable, inter alia, of processing the arrays created by the combination of NAPPA technology and ISFET architecture. | 12-04-2014 |
20140364337 | ABSOLUTE QUANTITATION OF PROTEINS AND PROTEIN MODIFICATIONS BY MASS SPECTROMETRY WITH MULTIPLEXED INTERNAL STANDARDS - A method for absolute protein or peptide quantitation by mass spectroscopy. A sample containing a protein or peptide of interest is prepared for mass spectroscopy analysis. The sample is subjected to mass spectroscopy analysis at low resolution whereby a single additive mass spectroscopy peak is obtained, then is subjected to high resolution mass spectroscopy analysis whereby a plurality of mass spectroscopy peaks are obtained. The intensity of each of the plurality of mass spectroscopy peaks is quantitated either by comparison to an internal standard set, or by using a standard curve generated for each isotopologue set. Quantitation using a standard curve enhances quantitation across a dynamic range of analyte. | 12-11-2014 |
20150011423 | MEANS AND METHODS FOR ASSESSING KIDNEY TOXICITY - The present invention pertains to the field of diagnostics for kidney toxicity and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing kidney toxicity. It also relates to a method for determining whether a compound is capable of inducing such kidney toxicity in a subject and to a method of identifying a drug for treating kidney toxicity. Furthermore, the present invention relates to a device and a kit for diagnosing kidney toxicity. | 01-08-2015 |
20150011424 | METHOD FOR DETERMINING LIVER FAT AMOUNT AND METHOD FOR DIAGNOSING NAFLD - The present invention is based on the idea of determining certain molecular lipids from a subject's blood sample, for example from serum or plasma sample, and based on the amounts of the determined lipids determining the amount of liver fat and/or diagnosing NAFLD in the subject. More specifically the subjects with elevated liver fat amount and NAFLD are characterized by elevated triglycerides with low carbon number and double bond content in the blood sample. Lysophosphatidylcholines, ether phospholipids, sphingomyelins and PUFA-containing phospholipids are diminished in the blood samples of subjects with an elevated liver fat amount and NAFLD. The method of the present invention can be further used for monitoring the subject's response to the treatment of NAFLD or to the treatment of lowering of the liver fat amount in the subject. | 01-08-2015 |
20150011425 | METHODS AND ARRAYS FOR CONTROLLED MANIPULATION OF DNA AND CHROMATIN FRAGMENTS FOR GENETIC AND EPIGENETIC ANALYSIS - The present invention relates to methods and arrays for use in high resolution imaging of individual nucleic acid molecules and chromatin fragments, including native chromatin fragments. In one aspect, the present invention relates to a chromatin array that includes a transfer platform having a support and a transfer surface layered on the support. The chromatin array also includes a plurality of elongated individual native chromatin fragments coupled to the transfer surface in an orderly pattern suitable for high resolution imaging of the plurality of native chromatin fragments. The native chromatin fragments of the chromatin array include both DNA and histones. | 01-08-2015 |
20150011426 | ASSAY, COMPOSITION AND NON-ENZYMATIC MECHANISM OF STATIN IN MODULATING LIPID METABOLISM - A novel non-enzymatic assay for lipid modulating drugs is being described for biological samples. For the first time non-enzymatic mechanism of statin drugs in modulating lipid aggregates and forming lipid particles is being shown. A simple flow cytometer based testing for detection of lipid particles, the effect of lipid modulating drug such as statin in vitro and testing for efficacy of the drug for an individual, dosage adjustment and drug discovery mechanism is being described. The lipid modulating drugs either individually or in combination can be discovered, optimized or efficacy tested using this assay. A novel statin induced lipid particle formation described can be used for diagnosis, personalized medicine and biomarker identification as well. | 01-08-2015 |
20150018244 | MEANS AND METHODS FOR ASSESSING HYPERTHYROIDISM - The present invention pertains to the field of diagnostics for hyperthyroidism and toxicological assessments for risk stratification of chemical compounds. Specifically, it relates to a method for diagnosing hyperthyroidism. It also relates to a method for determining whether a compound is capable of inducing such hyperthyroidism in a subject and to a method of identifying a drug for treating hyperthyroidism. Furthermore, the present invention relates to a device and a kit for diagnosing hyperthyroidism. | 01-15-2015 |
20150018245 | LIPID ARRAYS ON FABRICATED DEVICES AND USES THEREOF - Systems and methods for arranging microdevices on an array substrate are described. Each microdevice contains a lipid bilayer region and a patterned magnetic material that has a predetermined preferential axis of magnetization. The microdevices are located at specific locations on the arraying substrates and a magnetic field is used to orient the microdevices to facilitate the study of lipids and lipid-associated moieties. The bilayer region can also contain a fluorescent reagent, which can be monitored during exposure to an excitation source. The fluorescent reagent can also be photobleached and monitored for fluorescence recovery. | 01-15-2015 |
20150018246 | POLYPEPTIDE MARKER FOR DIAGNOSING AND ASSESSING VASCULAR DISEASES - The invention relates to a method for diagnosing vascular diseases, comprising the step in which the presence, absence or amplitude of at least three polypeptide markers is determined in a urine sample, wherein the polypeptide markers are selected from the markers characterized in table 1 by values for the molecular masses and the migration time. | 01-15-2015 |
20150024968 | ANALYTICAL DEVICES HAVING COMPACT LENS TRAIN ARRAYS - Apparatus, systems and methods for use in analyzing discrete reactions are provided. The analytical devices of the invention use an array of nanoscale regions (a chip) that has discrete patches of nanoscale regions. The chip mates with a collection device comprising an array of compact lens trains (CLTs) where each of the CLTs corresponds to a single patch of nanoscale regions. Each CLT collects the emitted light from a patch on the chip, collimates the light, performs color separation on the collimated emitted light, and focuses the separated light onto a portion of pixels on the detector below the CLT. Such systems are useful for monitoring many analytical reactions at one time including single molecule sequencing reactions. | 01-22-2015 |
20150024969 | SEPSIS PROGNOSIS BIOMARKERS - A method for determining prognosis, diagnosis and theronosis of a sepsis infection in a patient is disclosed. The method involves measuring the age, mean arterial pressure, hematocrit, patient temperature, and the concentration of one or more metabolites that are predictive of sepsis severity. The method can involve obtaining a blood sample from said patient and determining the concentration of the metabolite in the patient's blood; and then determining the severity of sepsis infection by analyzing the measured values in a weighted logistic regression equation. | 01-22-2015 |
20150024970 | KIDNEY CELL CARCINOMA - The process for the diagnosis of a renal cell carcinoma comprises the step of determining the presence or absence or amplitude of at least three polypeptide markers in a urine sample, wherein said polypeptide markers are selected from the markers as characterized in Table 1 by molecular masses and migration times. | 01-22-2015 |
20150031580 | METHOD FOR THE QUANTITATIVE ASSESSMENT OF GLOBAL AND SPECIFIC DNA REPAIR CAPACITIES OF AT LEAST ONE BIOLOGICAL MEDIUM, AND THE APPLICATIONS THEREFOR - A method for quantitative assessment of base excision repair (BER) and nucleotide excision repair (NER) DNA repair capacities of at least one cellular extract, which method entails: a) preparing a range of plasmids with at least one physical or chemical treatment or both and recovering a supercoiled fraction of each of said plasmids, b) characterizing the lesions present on each of the plasmids of the range of plasmids; c) depositing the plasmids of the range of plasmids, and at least one supercoiled control plasmid without lesions onto a single solid support, according to a pre-established configuration A, so as to form a functionalized support divided into different zones A1 to Ax, corresponding to an integer equal to the number of cellular extracts to be simultaneously tested, each zone containing supercoiled plasmids treated with at least one physical or chemical treatment that induces a lesion acted upon by BER mechanisms, and at least one other zone containing supercoiled plasmids treated with at least one physical or chemical treatment that induces a lesion acted upon by NER mechanisms; d) incubating the functionalized support obtained in step c) with various repair solutions, each of which contains at least one cellular extract from a subject, and labeled nucleotide phosphates; e) washing the functionalized support at least once; f) directly or indirectly measuring the signal produced by the labeled nucleotide triphosphate incorporated into the DNA during the repair reaction in step d), in each of the different and pre-established zones A | 01-29-2015 |
20150031581 | METHODS AND KITS FOR DETERMINING PROTEIN-BOUND BIOMARKERS - The invention relates to the field of diagnostic methods, in particular to detection of biological molecules using mass spectroscopy (MS). Provided is an automated high-throughput method for quantitating a low molecular weight protein-bound biomarker directly in an isolated biological sample, comprising the steps of:
| 01-29-2015 |
20150031582 | Volatile Organic Gases as Bioindicators for Transplant Rejection - A method comprising (a) obtaining one or more biological samples from a subject wherein the subject has undergone an organ transplant; (b) determining the amount and type of one or more volatile organic compounds in the biological sample; and (c) correlating the amount of volatile organic compounds to a degree of transplant rejection. A device comprising a plurality of sensors configured to detect a plurality of volatile organic compounds in a biological sample of a subject having undergone an organ transplant. | 01-29-2015 |
20150045256 | MASS SPECTROMETRY QUANTITATION OF P450 PROTEIN ISOFORMS IN HEPATOCYTES - A method for screening a drug for cytochrome P450 (CYP) induction is provided and can include incubating the drug with a microsome-containing biological sample and then quantitating at least one cytochrome P450 isoform. The isoforms can be selected from 2B6, 3A4, 1A2, and 3A5 isoforms. In some embodiments, the method uses liquid chromatography tandem mass spectrometry (LC-MSMS). A quantitated value can be compared to a threshold value and the drug can be determined to exhibit an acceptable CYP induction potential when the quantitated value does not exceed the threshold value. Isolated peptides are also provided. | 02-12-2015 |
20150051111 | ANALYSIS OF ESTRADIOL AND ANALYTES WITH PHENOLIC OH USING LABELING CHEMISTRY AND LC-MSMS WORKFLOW - A method is described for mass spectrometric analysis of a sample comprising phenolic OH, such as a steroid comprising a phenolic OH, using a quaternary amino oxy Cookson (QAOC) reagent. The QAOC reagent can improve ionization and fragmentation properties of phenolic OH samples, which can thereby improve quantitation and identification. The method can include derivatizing the phenolic OH sample with the QAOC reagent to create an adduct and analyzing the adduct using mass spectrometry. Derivatization of the sample can be a one-step reaction where the QAOC reagent comprises an aminooxy MS tag or can be a multi-step reaction, where the adduct is formed by the reaction of carbonyl substituted PTAD based reagent and the sample followed by combination with an aminooxy MS tag. The sample can also be enriched prior to reacting it with the reagent. The method can also allow for multiplexing. | 02-19-2015 |
20150065390 | INTEGRATED SENSORS - Examples of integrated sensors are disclosed herein. An example of an integrated sensor includes a substrate and a sensing member formed on a surface of the substrate. The sensing member includes collapsible signal amplifying structures and an area surrounding the collapsible signal amplifying structures that enables self-positioning of droplets exposed thereto toward the collapsible signal amplifying structures. | 03-05-2015 |
20150065391 | POLYPEPTIDE MARKERS FOR DIAGNOSIS AND ASSESSMENT OF HEART FAILURE - Method for diagnosis of heart failure, comprising the step of determining the presence or absence or amplitude of at least three polypeptide markers in a urine sample, the polypeptide markers being selected from the markers characterized in Table 1 by values for the molecular masses and the migration time. | 03-05-2015 |
20150072895 | SRM/MRM Assay for Subtyping Lung Histology - The current disclosure provides for specific peptides, and derived ionization characteristics of the peptides, from the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins that are particularly advantageous for quantifying the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins directly in biological samples that have been fixed in formalin by the method of Selected Reaction Monitoring (SRM) mass spectrometry, or what can also be termed as Multiple Reaction Monitoring (MRM) mass spectrometry. Such biological samples are chemically preserved and fixed wherein said biological sample is selected from tissues and cells treated with formaldehyde containing agents/fixatives including formalin-fixed tissue/cells, formalin-fixed/paraffin embedded (FFPE) tissue/cells, FFPE tissue blocks and cells from those blocks, and tissue culture cells that have been formalin fixed and or paraffin embedded. A protein sample is prepared from said biological sample using the Liquid Tissue™ reagents and protocol and the KRT5, KRT7, NapsinA, TTF1, TP63, and/or MUC1 proteins are quantitated in the Liquid Tissue™ sample by the method of SRM/MRM mass spectrometry by quantitating in the protein sample at least one or more of the peptides described. These peptides can be quantitated if they reside in a modified or an unmodified form. An example of a modified form of a KRT5, KRT7, NapsinA, TTF1, TP63, and MUC1 fragment peptide is phosphorylation of a tyrosine, threonine, serine, and/or other amino acid residues within the peptide sequence. | 03-12-2015 |
20150080263 | PRENATAL SCREENING FOR DOWN SYNDROME AND TRISOMY 18 - The present invention is directed to methods for predicting a pregnant woman's risk of carrying a fetus with Down syndrome (Trisomy 21) or Trisomy 18. The methods are based on measuring one or more metabolites obtained from a pregnant woman's bodily fluid, such as blood or urine, and found to be predictive of Trisomy 21 or Trisomy 18. | 03-19-2015 |
20150080264 | PC-O 44:6 - A BIOMARKER FOR VISCERAL ADIPOSITY - The present invention generally relates to the field of biomarkers. In particular, the present invention relates to biomarkers such as PC-O 44:6 that can be used, for example for detecting and/or quantifying visceral adiposity and/or changes in visceral adiposity. This biomarker may also be used to diagnosing the effect of a change in lifestyle on visceral adiposity in a subject. | 03-19-2015 |
20150087553 | EARLY TRIMESTER SCREENING FOR EARLY- AND LATE-ONSET PREECLAMPSIA - The present invention is directed to methods for predicting a pregnant woman's risk of developing early-onset preeclampsia or late-onset preeclampsia. The methods are based on measuring one or more metabolites obtained from a pregnant woman's bodily fluid, such as blood or urine, which were found to be predictive of early-onset preeclampsia and late-onset preeclampsia. | 03-26-2015 |
20150087554 | POLYPEPTIDE MARKERS FOR THE DIAGNOSIS OF ALZHEIMER'S DISEASE - A method for the diagnosis of the probability of Alzheimer's disease in a subject patient comprising the step of determining the presence or absence of at least three polypeptide markers in a sample, wherein the at least three polypeptide markers are at least three different markers selected from markers 1 to 50 (frequency markers) and markers 51 to 279 (amplitude markers), wherein the diagnosis is based on a comparison and ranking of the frequency and/or amplitude of the at least three polypeptide markers to the same at least three polypeptide markers obtained from control subjects with and without Alzheimer's disease. | 03-26-2015 |
20150094232 | MANAGING VARIATION IN SPECTROSCOPIC INTENSITY MEASUREMENTS THROUGH THE USE OF A REFERENCE COMPONENT - The present invention generally pertains to methods and kits for managing the variation in spectroscopic intensity measurements through the use of a reference component. The reference component may comprise a reference spectroscopic substance and may be contained together with a sample of interest in a sample to be tested, wherein the sample of interest may comprise a sample spectroscopic substance. Each sample to be tested may be uniquely identified and, hence, “barcoded” by combinations of different colors and concentrations of spectroscopic substances, contained therein. | 04-02-2015 |
20150094233 | ISOTOPE-LABELED PYRYLIUM COMPOUND - The present invention provides a compound represented by the formula (I): | 04-02-2015 |
20150094234 | Method for characterizing and/or determining samples - This invention relates to a method for characterizing and/or determining a samples, particularly in the aid of lanthanide(III) ions and ligands including an aromatic structure and 2-5 chelating heteroatoms. The invention relates also to an array for characterizing and/or determining a samples utilizing the properties of lanthanide(III) chelates. | 04-02-2015 |
20150099668 | METABOLITE BIOMARKERS FOR STAGING COLORECTAL CANCER - This document provides methods and materials for assessing metabolites in a sample from a mammal (e.g., human) for determining the nature and extent of colorectal cancer (CRC) metastasis. For example, the document relates to the diagnosis, staging and prognosis of CRC in a mammal. | 04-09-2015 |
20150099669 | MASS SPECTROMETRY IMAGING OF GLYCANS FROM TISSUE SECTIONS AND IMPROVED ANALYTE DETECTION METHODS - The presently disclosed subject matter provides methods using mass spectrometry for direct profiling of N-linked glycans from a biological sample. In addition, the embodiments of the present invention also disclose novel methods, known as targeted analyte detection (TAD), for improving the detection limit of MALDI-MS. These methods take advantage of the carrier effect of the added standard analytes, which occurs due to the generic sigmoidal shape of the calibration curve. The functionality of TAD depends on the relative enhancement of sensitivity over the increase of the standard deviation at the analysis of target analytes with spiking in exogenous concentration. At certain ranges of exogenous concentration, the increment in the sensitivity overcomes the standard deviation, resulting in an improved LOD. Theoretically, exogenous concentrations approximately at 1 LODorig would generate the optimum LOD improvement. TAD is a cost-effective LOD improvement method, which is not limited to a certain group of analytes, or detection methods or instruments. It can be applied to enhance the detection of any analyte with different detection methods, provided that the analyte of interest can be extracted or is available in synthetic form. | 04-09-2015 |
20150105296 | HYDROXY-SPHINGOMYELIN 22:1 AS A BIOMARKER FOR HEALTHY AGING - Using NMR/MS based metabonomics and targeted lipidomics approaches the inventors have explored the metabolic phenotypes of aging and longevity in a cohort compromising centenarians, elderly and young adults. The inventors have identified biomarkers for a reduced risk of developing ageing related chronic inflammatory disorders and propose a method of diagnosing a lifestyle that allows delaying and/or avoiding ageing related chronic inflammatory disorders using hydroxy-sphingomyelin SM-OH-22:1 as biomarker. | 04-16-2015 |
20150105297 | Electrical Polynucleotide Mapping - A micro-fluidic device for mapping a DNA or RNA strand labeled at a plurality of specific sites with labels suitable for generating a detection signal when interacting with a detector element, the device comprising:
| 04-16-2015 |
20150111787 | DIGITAL MICROFLUIDIC CHIPS FOR AUTOMATED HYDROGEN DEUTERIUM EXCHANGE (HDX) MS ANALYSIS - Described herein is a digital microfluidic droplet generator (DMDG) and a microfluidic platform for processing material introduced into the DMDG. The combination is particularly suited for hydrogen/deuterium exchange and mass spectrometer (HDX-MS) processing and analysis of membrane proteins. | 04-23-2015 |
20150119285 | MAGNETIC-ASSISTED RAPID APTAMER SELECTION METHOD FOR GENERATING HIGH AFFINITY DNA APTAMER - A magnetic-assisted rapid aptamer selection (MARAS) protocol for screening DNA aptamer is proposed. The MARAS protocol is able to efficiently generate aptamers with high affinity and specificity. A rotating magnetic field or alternating magnetic field was used in combination with target-bound magnetic micro-particles or nanoparticles to select DNA aptamers having desirable affinity and specificity to the target. | 04-30-2015 |
20150119286 | HIGH-THROUGHPUT CORROSION TESTING PLATFORM - A high-throughput corrosion testing mechanism was developed for metals in a variety of environments in controlled, multiplexed microenvironments. Many parallel experiments can be conducted with microbial and environmental variables independently manipulated to identify the key determinants of corrosion progression. The synthetic assay design enables subsequent surface characterization of select samples within the array. In as little as one day, diverse corrosive environments can be compared quantitatively. | 04-30-2015 |
20150126402 | Method for Quantifying Proteins and Isoforms Thereof - A method for quantifying metallothionein protein isomers is described herein. Such metallothionein isomer protein quantification is useful for detecting and monitoring disease. As illustrated herein, protein quantification is a more accurate measure of metallothionein induction than is mRNA quantification. | 05-07-2015 |
20150126403 | METHOD OF IDENTIFYING PROTEINS IN HUMAN SERUM INDICATIVE OF PATHOLOGIES OF HUMAN LUNG TISSUES - A method of identifying proteins present in human serum which are differentially expressed between normal individuals and patients known to have non-small cell lung cancers and asthma, as diagnosed by a physician. Human serum specimens from each population are digested with trypsin or any other suitable endoproteinase and analyzed using a liquid chromatography electrospray ionization mass spectrometer. Mass spectral data from each population is compared to determine proteins with expression intensities which are significantly differentially expressed between the normal, asthma, and lung cancer populations. Eleven proteins are found to have expression intensities which are significantly differentially expressed between the populations. Finally, the identities of the eleven proteins are obtained by comparing the mass spectral data with known databases having libraries of mass spectral data of known proteins. | 05-07-2015 |
20150126404 | CELLARIUM: THIN-FILM SENSOR WITH MICROARRAY SEAL - A triple sensor structured for simultaneous measurement of glucose, oxygen, and pH. The sensor components are in thin film states such as sensing films or membranes, with a glucose probe associated with emission of radiation in the blue part of the spectrum, an oxygen probe associated with radiation in red portion of the spectrum, and a pH probe—with a green portion of the spectrum. The optical probes are chemically grafted or immobilized in a suitable polymer matrix, alleviating the leaching of the probes from the matrix, improving the thin film sensing stability, and enabling the repeatable use of the same sensing films. | 05-07-2015 |
20150126405 | POLYPEPTIDE MARKERS FOR THE EARLY RECOGNITION OF THE REJECTION OF TRANSPLANTED KIDNEYS - A method for recognizing rejection after a kidney transplantation (NTx), comprising the step of determining the presence or absence of at least one polypeptide marker in a sample, wherein said polypeptide marker is selected from markers 1 to 242 (frequency markers), or determining the amplitude of at least one polypeptide marker selected from markers 243 to 767 (amplitude markers), which are characterized by the values for the molecular masses and migration times (CE time). | 05-07-2015 |
20150133342 | MRM-MS SIGNATURE ASSAY - The present invention relates to mass spectrometry methods employing multiple reaction monitoring (MRM) in the field of cancer therapeutics, specifically prostate cancer and melanoma. | 05-14-2015 |
20150133343 | POLYPEPTIDE MARKERS FOR THE DIAGNOSIS OF PROSTATE CANCER - A method for the diagnosis of prostate cancer, comprising the step of determining the presence or absence of at least three polypeptide markers in a sample, wherein the polypeptide marker is selected from markers 1 to 44 and 52 to 78 (frequency markers), or determining the amplitude of at least one polypeptide marker selected from markers 45 to 51 and 79 to 115 (amplitude markers), wherein said sample is a urine sample or seminal fluid sample. | 05-14-2015 |
20150141290 | METHOD FOR QUANTIFICATION OF PROTEOME - The present invention relates to a method for quantifying the relative content of a protein in a sample. The present invention also relates to a method for comprising the relative content of a protein in at least two samples. | 05-21-2015 |
20150141291 | SELF-CONTAINED BIOLOGICAL ASSAY APPARATUS, METHODS, AND APPLICATIONS - A self-contained, fully automated, biological assay-performing apparatus includes a housing; a dispensing platform including a controllably-movable reagent dispensing system, disposed in the housing; a reagent supply component disposed in the housing; a pneumatic manifold removably disposed in the housing in a space shared by the dispensing platform, removably coupled to a fluidic transport layer and a plurality of reservoirs, wherein the fluidic transport layer, the reservoirs, and a test sample to be introduced therein are disposed in the housing in the space separate from the dispensing platform; a pneumatic supply system removably coupled to the pneumatic manifold in the housing in a space separate from the dispensing platform; and a control system coupled to at least one of the dispensing platform and the pneumatic supply system, disposed in the housing. | 05-21-2015 |
20150148257 | DETECTING ANALYTES - Provided is a method for detecting analyte in a sample, which method comprises:
| 05-28-2015 |
20150148258 | METHOD FOR SIMULTANEOUS DETECTION, RECOVERY, IDENTIFICATION AND COUNTING OF MICROORGANISMS AND DEVICES FOR THE IMPLEMENTATION OF SAID METHOD - The present invention describes a method and devices for the simultaneous detection, recovery identification and counting of a plurality of microorganisms consisting in providing mixtures of nutrients specially selected from those that curtail the lag phase of growth in bacteria and moulds and which, together with fluorescent enzymatic, chromogenic or bioluminescent markers and other nutrient components or growth inhibitors, are embedded in three-dimensional structures or natural or artificial clays or ceramics with cavities of different dimensions and forms and specific surface areas of between 2×103 and 6×108 m | 05-28-2015 |
20150148259 | NOVEL PHENYL GLYOXAL PROBES - Novel phenyl-glyoxal based anti-citrulline probes and methods of synthesis are provided. Methods of use, such as, the development of methods for monitoring substrate citrullination over time; for identifying citrullinated proteins from cells are described. | 05-28-2015 |
20150148260 | ANALYTE MASS SPECTROMETRY QUANTITATION USING A UNIVERSAL REPORTER - Quantitation of analytes, including but not limited to peptides, polypeptides, and proteins, in mass spectrometry using a labeled peptide coupled to a reporter, and a universal reporter. | 05-28-2015 |
20150148261 | SYSTEMS AND METHODS FOR DETECTION OF CELLS USING ENGINEERED TRANSDUCTION PARTICLES - Systems and methods for detecting and/or identifying target cells (e.g., bacteria) using engineered transduction particles are described herein. In some embodiments, a method includes mixing a quantity of transduction particles within a sample. The transduction particles are associated with a target cell. The transduction particles are non-replicative, and are engineered to include a nucleic acid molecule formulated to cause the target cell to produce a series of reporter molecules. The sample and the transduction particles are maintained to express the series of the reporter molecules when target cell is present in the sample. A signal associated with a quantity of the reporter molecules is received. In some embodiments, a magnitude of the signal is independent from a quantity of the transduction particle above a predetermined quantity. | 05-28-2015 |
20150293060 | SENSOR ARRAY CHIP WITH PIEZOELECTRIC TRANSDUCER INCLUDING INKJET FORMING METHOD - A method of forming a functionalized sensor array includes providing a substrate having at least one sensor array chip including a plurality of sensor structures. The sensor structures include a piezoelectric layer interposed between upper and lower electrodes and positioned across an area of the sensor array chip in a spatial arrangement. An inkjet cartridge chip is also provided having a plurality of microfluidic channels including a fill side having a plurality of fill side orifices and a dispense side having a plurality of dispense nozzles, wherein two or more of the plurality of microchannels are loaded with different sensing materials, and wherein locations of the plurality of dispense nozzles are matched to the spatial arrangement. The plurality of dispense nozzles are aligned to the plurality of sensor structures, and the plurality of dispense nozzles are actuated to deposit the different sensing materials on the plurality of sensor structures. | 10-15-2015 |
20150294076 | SYSTEM AND METHOD FOR SERUM BASED CANCER DETECTION - A system and method for analyzing biological samples, such as dried human blood serum, to determine a disease state such as colorectal cancer (CRC). Using dried samples may hold potential for enhancing localized concentration and/or segmentation of sample components. The method may comprise illuminating at least one location of a biological sample to generate a plurality of interacted photons, collecting the interacted photons and generating at least one Raman data set representative of the biological sample. A system may comprise an illumination source to illuminate at least one location of a biological sample and generate at least one plurality of interacted photons, at least one mirror for directing the interacted photons to a detector. The detector may be configured to generate at least one Raman data set representative of the biological sample. The system and method may utilize a FAST device for multipoint analysis or may be configured to analyze a sample using a line scanning configuration. | 10-15-2015 |
20150294081 | METHODS AND SYSTEMS FOR DETERMINING AUTISM SPECTRUM DISORDER RISK - In certain embodiments, the invention stems from the discovery that analysis of population distribution curves of metabolite levels in blood can be used to facilitate predicting risk of autism spectrum disorder (ASD) and/or to differentiate between ASD and non-ASD developmental delay (DD) in a subject. In certain aspects, information from assessment of the presence, absence, and/or direction (upper or lower) of a tail effect in a metabolite distribution curve is utilized to predict risk of ASD and/or to differentiate between ASD and DD. | 10-15-2015 |
20150300953 | METHODS, SYSTEMS, AND COMPUTER READABLE MEDIA FOR DETERMINING PHYSICAL PROPERTIES OF A SPECIMEN IN A PORTABLE POINT OF CARE DIAGNOSTIC DEVICE - Methods, systems, and computer readable media for determining physical properties of a specimen in a portable point of care device are disclosed. According to one aspect, a method includes placing a specimen onto an active surface that includes a plurality of microposts extending outwards from a substrate, wherein each micropost includes a proximal end attached to the substrate and a distal end opposite the proximal end and generating an actuation force in proximity to the micropost array that compels at least some of the microposts to exhibit motion. The method further includes detecting light that is emitted by an illumination source and interacts with the active surface while the at least some microposts exhibit motion in response to the actuation force, measuring data that represents the detected light interacting with the active surface, and determining at least one physical property of the specimen based on the measured data. | 10-22-2015 |
20150316483 | DEVICE AND METHODS FOR DETECTION OF ANALYTES INCLUDING USE OF A COLORIMETRIC BARCODE - Embodiments described herein related to devices and methods for the collection and/or determination of analytes, such as illicit substances including military explosives, explosives, and precursors thereof. In some cases, the device may be a disposable device that incorporates highly efficient sample collection in combination with microfluidic-based chemical analysis resulting in the rapid detection and identification of unknown materials. In some cases, multiple colorimetric detection chemistries may be employed, and the resulting “barcode” of color changes can be used to positively identify the presence and/or identity of the analyte. | 11-05-2015 |
20150316547 | Electrophoretic Separation Devices and Methods for Using the Same - Electrophoretic separation devices and methods for using the same are provided. Aspects of the devices include a polymeric separation medium that includes a plurality of microwells. Also provided are methods, systems and kits in which the subject devices find use. The devices and methods find use in a variety of different electrophoretic separation applications. | 11-05-2015 |
20150323545 | SELECTED REACTION MONITORING ASSAYS - Provided herein are methods for developing selected reaction monitoring mass spectrometry (LC-SRM-MS) assays. | 11-12-2015 |
20150323554 | ANALYSIS OF A PANEL OF CEREBROTENDINOUS XANTHOMATOSIS BIOMARKERS USING SITE SPECIFIC DERIVATION AND LC/MS/MS WORKFLOW - A method, a labeling reagent, sets of labeling reagents, and labeling techniques are provided for the analysis of ketosterol biomarkers such as bile acid precursors from human plasma, serum or whole blood. This method is used for new born screening for Cerebrotendinous Xanthomatosis (CTX). Methods for labeling, analyzing, and quantifying ketosterol biomarkers are also disclosed as are methods that also use mass spectrometry. | 11-12-2015 |
20150337351 | METHODS OF MICROORGANISM IMMOBILIZATION - The present disclosure is related to methods for immobilizing microorganisms to produce an immobilized microorganism sample for detection with a detection system. Compositions for immobilizing microorganisms are also disclosed. | 11-26-2015 |
20150337360 | DEVICE FOR GENOTYPIC ANALYSIS AND METHOD FOR GENOTYPIC ANALYSIS - A technique for performing spectral calibration simultaneously with electrophoresis of an actual sample to be analyzed, without performing electrophoresis using a special matrix standard which is time-consuming and costly, is provided. The device for genotypic analysis is characterized by obtaining reference fluorescence spectra using a size standard and an allelic ladder, which provide information concerning known DNA fragments used for electrophoresis of an actual sample, and is characterized by performing spectral calibration for a capillary in which the allelic ladder is not used by detecting a shift amount of the fluorescence spectra of the size standard and shifting the reference fluorescence spectra using the shift amount to determine fluorescence spectra. | 11-26-2015 |
20150338362 | Combinatorial screening of metallic diffusion barriers - Barrier layers, barrier stacks, and seed layers for small-scale interconnects (e.g., copper) are combinatorially screened using test structures sputtered or co-sputtered through apertures of varying size. Various characteristics (e.g., resistivity, crystalline morphology, surface roughness) related to conductivity, diffusion blocking, and adhesion are measured before and/or after annealing and compared to arrive at materials and process parameters for low diffusion with high conductivity through the interconnect. Example results show that some formulations of tantalum-titanium barriers may replace thicker tantalum/tantalum-nitride stacks, in some cases with a Cu—Mn seed layer between the Ta—Ti and copper. | 11-26-2015 |
20150338389 | DIAGNOSING MULTIPLE SCLEROSIS - There is provided an in vitro method for diagnosing Multiple Sclerosis (MS) in a human test subject, comprising (i) determining the concentrations of two or more metabolites in a sample from said subject, wherein said two or more metabolites are selected from: blood metabolites, wherein said blood metabolites comprise: alanine, ascorbic acid, choline, fatty acid, glucose, lactate, N-acetyl aspartate, N-acetyl glycoprotein, n-butyrate, oxyglutaric acid, phosphocholine, taurinebetaine, tyrosine, L-glutamine, N-acetyl species, and beta-hydroxybutyrate; and/or urine metabolites, wherein said urine metabolites comprise: citrate, creatinine, inositol, lactate and trimethylamine N-oxide (TMAO); (ii) comparing the concentrations of said two or more metabolites in the sample with the concentrations of the same metabolites in at least one reference standard; and (iii) identifying a concentration difference for each of said two or more metabolites in the sample relative to the reference standard; wherein said concentration differences correlate with the presence of MS. | 11-26-2015 |
20150353921 | METHOD OF ON-CHIP NUCLEIC ACID MOLECULE SYNTHESIS - A method of synthesizing a nucleic acid molecule, such as a gene, on a substrate or microchip is described. In particular, a method for synthesizing, amplifying, and assembling DNA oligonucleotides into a nucleic acid molecule or gene product, on a single substrate or microchip is described. Also described are a method of correcting a sequence error in a synthesized nucleic acid molecule, as well as a method for synthesizing and screening a library of codon variants to identify a nucleic acid molecule with an optimized level of protein expression. | 12-10-2015 |
20150355147 | HIGH THROUGHPUT QUANTIFICATION AND CHARACTERIZATION OF FOOT AND MOUTH DISEASE VIRUS AND PRODUCTS THEREOF - The present invention provides a high throughput method to quantify and characterize the size and integrity of viruses and viral molecules. In one embodiment, the present invention provides a method to quantify and characterize size and integrity of Foot and Mouth Disease virus (FMDV) using chromatographic system and in-line Dynamic Light Scattering (DLS) technique. In one embodiment, the present invention further comprises a column-switching system for running multiple analyses simultaneously. The present invention also provides a method to develop and evaluate FMDV containing products for preventing Foot and Mouth Disease (FMD). In one embodiment, the methods described herein assess the stability of FMDV. In another embodiment, the methods described herein serve as in-process quality control for a manufacturing process of FMD vaccine. | 12-10-2015 |
20150355190 | Compositions and Methods of Analysis - The present disclosure provides compositions and methods for performing analysis on a sample. | 12-10-2015 |
20150362431 | MODULAR ASSAY SYSTEM - A system for conducting an assay comprises a power source ( | 12-17-2015 |
20150362513 | CERAMIDES AND THEIR USE IN DIAGNOSING CVD - The present invention inter alia provides a method, and use thereof, of predicting CV complications such as AMI, ACS, stroke, and CV death by determining the concentrations of at least one ceramide of Group A and at least one ceramide of Group B in a biological sample and comparing those concentrations to a control. Finding a decreased concentration of at least one Group A ceramide and an increased concentration of at least one Group B ceramide indicates that the subject has an increased risk of developing one or more CV complications. Also provided are a newly identified subset of ceramide molecules, labelled versions thereof, and kits and compositions comprising the same for use in predicting and/or diagnosing CV complications. | 12-17-2015 |
20150369791 | DEVICES AND SYSTEMS FOR MIMICKING HEART FUNCTION - In an aspect, disclosed herein are physiological devices and systems, and components thereof, used to evaluate cardiac parameters and arrhythmogenic mechanisms. This abstract is intended as a scanning tool for purposes of searching in the particular art and is not intended to be limiting of the present invention. | 12-24-2015 |
20150369825 | Diagnosis of Alzheimer Disease, Cognitive Decline and Dementia - The present embodiments relate to the usage of selected protein-derived glycans as biomarkers for diagnosis of Alzheimer disease, cognitive decline and dementia. | 12-24-2015 |
20150376684 | TEST KIT AND METHOD FOR RAPIDLY DETECTING LIVE BACTERIUM AND TEST KIT AND METHOD FOR RAPIDLY DETERMINING APPROPRIATE ANTIBIOTIC - A test kit for rapidly detecting a live bacterium is disclosed. The test kit for rapidly detecting a live bacterium includes ethidium monoazide, a magnetic bead, a first primer pair and a microfluidic chip. A test kit for rapidly determining an appropriate antibiotic is also disclosed. The test kit for rapidly determining an appropriate antibiotic includes ethidium monoazide, a magnetic bead, four primer pairs and a microfluidic chip. | 12-31-2015 |
20160003797 | METHOD FOR DETECTION OF ADENOSINE AND METABOLITES THEREOF - This invention relates is a label-free, enzyme-free, aptamer-free method for simultaneously measuring adenosine, and its intracellular metabolites, e.g., AMP, ADP and ATP, using high pressure liquid chromatography coupled to electrochemical detector (HPLC-ECD). | 01-07-2016 |
20160008785 | DEVICES AND METHODS FOR PRODUCING AND ANALYZING MICROARRAYS | 01-14-2016 |
20160016180 | DEVICES, SYSTEMS, AND METHODS FOR ACOUSTICALLY-ENHANCED MAGNETOPHORESIS - The present disclosure provides systems and devices for the sorting of objects using acoustic waves and magnetic forces as well as methods of using the systems and devices. | 01-21-2016 |
20160025637 | MICROWELL ARRAY ARTICLES AND METHODS OF USE - The disclosure provides microstructured articles and methods useful for detecting an analyte in a sample. The articles include microwell arrays. The articles can be used with an optical system component in methods to detect or characterize an analyte. | 01-28-2016 |
20160025709 | HIGH THROUGHPUT DNA DAMAGE QUANTIFICATION OF HUMAN TISSUE WITH HOME-BASED COLLECTION DEVICE - Kits, methods and systems for providing a service to provide a subject with information regarding the state of a subject's DNA damage. Collection, processing and analysis of samples are also described. | 01-28-2016 |
20160032281 | FUNCTIONALIZED GRIDS FOR LOCATING AND IMAGING BIOLOGICAL SPECIMENS AND METHODS OF USING THE SAME - A functionalized specimen support for use in charged particle microscopy is provided that includes a specimen support surface configured to support specimens during an interrogation of the specimens with a charged particle microscope, the specimen support surface having functionalized sites, each functionalized site configured to maintain position of a portion of one of the specimens at the functionalized site by way of attachment, attraction, or a combination thereof. | 02-04-2016 |
20160041140 | USE OF SILK NUMBER ASSAY TO SCREEN FOR GENES ENHANCING CORN YIELD - The invention provides a rapid and efficient method and assay for monitoring yield enhancement in a plant using a measure of silk number. The plant is transformed with a prospective gene associated with yield enhancement. The transformed plant is grown along with non-transformed control plants until silk growth is apparent. The change in the number of silks in the transformed plant correlated to the change in yield for the plant. Therefore, changes in yield enhancement can be estimated in the transformed plant prior to harvest of mature plant tissues. | 02-11-2016 |
20160046992 | CHARACTERIZATION OF MOLECULES IN NANOFLUIDICS - Methods are provided for detecting and quantitating molecules using fluidics. In preferred embodiments, the methods comprise analyzing blood to detect the presence of circulating DNA or cells from a fetus or tumor. | 02-18-2016 |
20160047829 | Means and Methods for Determining a Clearance Normalized Amount of a Metabolite Disease Biomarker in a Sample - The present invention relates to a method for determining a clearance normalized amount of a metabolite disease biomarker in a sample including the steps of (a) determining the amount of the disease biomarker in at least a first type of sample of a subject suspected to suffer from the disease, (b) determining the amount of a kidney function biomarker which correlates with the glomerular filtration rate (GFR) in the said at least first type of sample, and (c) determining a clearance normalized amount for the metabolite disease biomarker by normalizing the amount determined for the metabolite disease biomarker in step (a) to the amount of the kidney function biomarker determined in step (b). Moreover, the invention also relates to a method for diagnosing a disease in a subject suspected to suffer therefrom and to a device for determining a clearance normalized amount of a metabolite disease biomarker in a sample. | 02-18-2016 |
20160054229 | PAPER SENSING AND ANALYTIC SERVICE WORKFLOW METHODS AND SYSTEMS - This disclosure provides a workflow method and system associated with a paper-based sensor. Specifically, provided is a paper-based sensor workflow including printing of customized security sensing information and bio-reagents to produce a paper-based sensor, applying a test material, such as, but not limited to, blood, to the paper-based sensor, capturing an image of the paper-based sensor and performing a colorimetric process; and performing one or more analytics to produce results associated with the test material | 02-25-2016 |
20160059204 | SYSTEM FOR MIXING FLUIDS BY COALESCENCE OF MULTIPLE EMULSIONS - System, including methods, apparatus, compositions, and kits, for the mixing of small volumes of fluid by coalescence of multiple emulsions. | 03-03-2016 |
20160061848 | MASS SPECTROMETRY OF STEROIDAL COMPOUNDS IN MULTIPLEXED PATIENT SAMPLES - The invention relates to the quantitative measurement of steroidal compounds by mass spectrometry. In a particular aspect, the invention relates to methods for quantitative measurement of steroidal compounds from multiple samples by mass spectrometry. | 03-03-2016 |
20160068897 | APPARATUS AND METHOD FOR EXTRACTING MICROBIAL CELLS - Methods and devices are provided for pretreatment of a sample containing microbial cells. In some embodiments, the pretreatment of the sample is performed via the initial selective lysis, within a sample pretreatment vessel, of non-microbial cells (such as blood cells) and the subsequent centrifugal separation of the sample to remove the resulting debris and concentrate the microbial cells. An immiscible and dense cushioning liquid may be included for collecting the microbial cells adjacent to the liquid interface formed by the cushioning liquid upon centrifugation of the pretreatment vessel. After removal of a substantial quantity of the supernatant, resuspension of the collected microbial cells, and re-establishment of the cushioning liquid interface, at least a portion of the remaining suspension may be removed without substantially removing the cushioning liquid. One or more intermediate wash cycles may be performed prior to extraction of the remaining suspension, which provides a “pretreated” sample. | 03-10-2016 |
20160077116 | CARDIOVASCULAR RISK EVALUATIONS USING A RISK PARAMETER THAT INCLUDES AN HDL AND INFLAMMATORY BIOMARKER INTERACTION PARAMETER - Methods, systems and circuits evaluate a subject's CVD risk using a risk parameter that includes at least one HDL and inflammatory biomarker interaction parameter. The inflammatory biomarker may optionally comprise NMR derived measurements of GlycA from at least one biosample of the subject. The risk parameter may be gender-specific. | 03-17-2016 |
20160084772 | DISPOSABLE AND DISPERSIBLE EXPLOSIVE DETECTION DEVICE AND METHOD OF SIMULTANEOUS DETECTION OF EXPLOSIVES - The present invention relates to a method and utility device for detecting explosives selected from inorganic nitrates like ammonium nitrate (AN), chlorates, nitramines like cyclo trimethylene trinitramine (RDX), Her Majesty's Explosive (HMX), tetryl (CE), nitrate esters like pentaerythritol tetranitrate (PETN), nitro compounds like trinitrotoluene (TNT) plastic explosives like Semtex, TATB and combinations thereof. It also relates to two variants of explosive detection kit viz. a readily portable miniaturized disposable drop type variant and a dispersible spray type variant. | 03-24-2016 |
20160084857 | FATTY ACID PATTERN ANALYSIS FOR PREDICTING ACUTE CORONARY SYNDROME - The present invention provides blood based methods for predicting risk of acute coronary syndrome in a subject. | 03-24-2016 |
20160089649 | Multi-through Hole Testing Plate for High Throughput Screening - A method for holding samples for analysis and an apparatus thereof includes a testing plate with a pair of opposing surfaces and a plurality of holes. Each of the holes extends from one of the opposing surfaces to the other one of the opposing surfaces. The holes are arranged in groups, where each group has at least two rows and two columns of holes. The groups are arranged in sets, where each set has at least two rows and two columns of groups. To analyze samples, at least one of the opposing surfaces of the testing plate is immersed in a solution to be analyzed. A portion of the solution enters openings for each of the holes in the immersed opposing surface. Once the holes are filled with solution, the testing plate is removed and is held above a supporting surface. Surface tension holds the solution in each of the holes. The solution in one or more of the holes is then analyzed and the solution in one of these holes is identified for further study. The location of the identified solution is marked based upon its location within a particular set and group of holes. | 03-31-2016 |
20160091480 | METHODS AND SYSTEMS FOR DETERMINING AUTISM SPECTRUM DISORDER RISK - In certain embodiments, the invention stems from the discovery that analysis of population distribution curves of metabolite levels in blood can be used to facilitate predicting risk of autism spectrum disorder (ASD) and/or to differentiate between ASD and non-ASD developmental delay (DD) in a subject. In certain aspects, information from assessment of the presence, absence, and/or direction (upper or lower) of a tail effect in a metabolite distribution curve is utilized to predict risk of ASD and/or to differentiate between ASD and DD. | 03-31-2016 |
20160097084 | KINASE ACTIVITY DETECTION METHODS - A strategy to take advantage of time-resolved luminescence of Ln | 04-07-2016 |
20160103068 | HIGH-THROUGHPUT FLUORESCENCE IMAGING SYSTEM AND DEVICE WITH SAMPLE HEATING CAPABILITY, AND ASSOCIATED METHODS - A high-throughput fluorescence imaging system with sample heating capability includes an image sensor wafer with a plurality of image sensors for fluorescence imaging a plurality of samples disposed in a respective plurality of fluidic channels on the image sensor wafer. The high-throughput fluorescence imaging system further includes a heating module, thermally coupled with the image sensor wafer, for heating the samples. A method for high-throughput assay processing includes modulating temperature of a plurality of samples disposed in a respective plurality of fluidic channels on an image sensor wafer by heating the image sensor wafer, using a heating module thermally coupled with the image sensor wafer, to control reaction dynamics in the samples; and capturing a plurality of fluorescence images of the samples, using a respective plurality of image sensors of the image sensor wafer, to detect one or more components of the plurality of samples. | 04-14-2016 |
20160103118 | IDENTIFYING DESIRABLE T LYMPHOCYTES BY CHANGE IN MASS RESPONSES - In certain embodiments methods of identifying T cell receptors that respond to specific target cell antigens are provided, where the methods comprise providing a substrate bearing a plurality of target cells (e.g., mammalian cells); contacting the target cells on the substrate with CD8+ T cells; and using label-free optical imaging to identify an increase in mass of a T-cell and/or a decrease in mass of a target cell, where an increase in mass of a T cell and/or a decrease in mass of a target cell is an indicator that said T cell bears a T cell receptor activated by antigens presented on said target cell. | 04-14-2016 |
20160103144 | BLOOD BASED METHODS OF ASSESSING ADOLESCENT DEPRESSION IN A SUBJECT - The present invention provides blood based methods for assessing adolescent depression in a subject. | 04-14-2016 |
20160109352 | METHODS AND APPARATUS FOR HIGH-THROUGHPUT LABEL-FREE CELL ASSAY - The present invention relates to a high-throughput label-free cell assay system which scans a lower part of medium made of glass using an object lens and a Galvano mirror and using a broadband laser as a light source, analyzes and visualizes light reflected from the lower part of the medium using a spectrometer, measures interference spectra formed by the light reflected from each interface of the medium using the spectrometer, and measures phase from data that the measured interference spectra were converted by Fourier transform to observe structural change of cells. The system includes a transparent medium on which a sample is put, an object lens located beneath the transparent medium; a Galvano mirror located beneath the object lens; an optical fiber coupler transferring light from the super-continuum light source to the Galvano mirror; and the spectrometer detecting spectrum of the light from the optical fiber coupler. | 04-21-2016 |
20160109368 | NUCLEIC ACID ANALYZER AND NUCLEIC ACID ANALYSIS METHOD USING SAME - Provided is a nucleic acid analyzer, which does not require manual processes by a highly trained operator such as a researcher and is easy to use, small-sized, capable of accepting multiple samples, and performs speedy analysis, and a nucleic acid analysis method using the analyzer. The analyzer and method perform detection in a plurality of exposure times, provide a program for determining a threshold for signal detection, and determine whether a faint signal peak is a false signal peak. | 04-21-2016 |
20160109424 | SWATH.TM. Data-Independent Acquisition Technology for the Detection of Host Cell Protein Contaminants in Biotherapeutics Protein Products - Systems and methods are provided for detecting host cell contaminants in a protein biotherapeutic product using sequential windowed acquisition tandem mass spectrometry. Sequential windowed acquisition is performed on a protein biotherapeutic product sample by sequentially stepping a precursor mass window across a mass range, fragmenting transmitted precursor ions of each stepped precursor mass window, and analyzing product ions produced from the fragmented transmitted precursor ions. The sequential windowed acquisition is performed without any information about contaminating proteins before data acquisition, and produces data for every product ion of every transmitted precursor ion for the mass range. One or more measured product ion spectra are received, and compared to a library of host cell proteins. One or more host cell contaminants are detected by reporting host cell proteins from the library that match the one or more measured product ion spectra. | 04-21-2016 |
20160116461 | Biomarkers Related To Metabolic Age and Methods Using The Same - Biomarkers relating to metabolic age are provided, as well as methods for using such biomarkers as biomarkers for determining metabolic age. In addition, methods for modulating the metabolic age of a subject are also provided. Also provided are suites of small molecule entities as biomarkers for metabolic age. | 04-28-2016 |
20160123913 | SYSTEMS AND METHODS OF SENSING AND/OR HEATING USING NANOSTRUCTURES - Various methods for sensing and/or heating that utilize nanostructures or carbon structures, such as nanotubes, nanotube meshes, or graphene sheets, are disclosed. In some methods, at least a pair of contacts are electrically coupled with a given nanostructure or carbon structure to sense a change or to pass a current for heating. | 05-05-2016 |
20160129415 | MULTIPLEX CHEMOTYPING MICROARRAY (MCM) SYSTEM AND METHODS - A Multiplex Chemotyping Microarray (MCM) system and methods are herein described. The MCM system and methods enable rapid chemical analyses of heterogeneous mixtures, by combining high-throughput micro-contact printing technology with high-fidelity (mass) vibrational spectroscopy. The MCM enables an error-free deposition and detection of multiple chemicals in a heterogeneous liquid sample at a throughput of more than two orders of magnitude beyond existing methods. | 05-12-2016 |
20160131667 | LIPID BIOMARKERS FOR STABLE AND UNSTABLE HEART DISEASE - The present invention relates generally to the field of diagnostic and prognostic assays for heart disease. More particular, the present invention provides an assay for diagnosing the presence or extent of development of heart disease or its classification or state thereof. The assay of the present invention is also useful in the stratification of a subject with respect to a risk of developing heart disease. The assay of the present invention is also capable of integration into pathology architecture to provide a diagnostic and reporting system. | 05-12-2016 |
20160139019 | METHOD AND INTEGRATED DEVICE FOR ANALYZING LIQUID FLOW AND LIQUID-SOLID INTERFACE INTERACTION - An Integrated Circuit (IC) chip with a lab-on-a-chip, a method of manufacturing the lab-on-a-chip and a method of using the lab-on-a-chip for fluid flow analysis in physical systems through combination with computer modeling. The lab-on-a-chip includes cavities in a channel layer and a capping layer, preferably transparent, covering the cavities. Gates control two dimensional (2D) lattice structures acting as heaters, light sources and/or sensors in the cavities, or fluid channels. The gates and two dimensional (2D) lattice structures may be at the cavity bottoms or on the capping layer. Wiring connects the gates and the 2D lattice structures externally. | 05-19-2016 |
20160139102 | PAPER MICROFLUIDIC DEVICES FOR DETECTION OF IMPROVISED EXPLOSIVES - Paper microfluidic devices for testing for explosives are provided, along with methods of fabricating and using the same. One or more channels are formed on a paper substrate, and a test spot is formed in at least one of the channels. The channels can be hydrophobic. A test reagent is provided in the test spot and tests for explosives. | 05-19-2016 |
20160139154 | METHODS FOR DETERMINING TOTAL BODY SKELETAL MUSCLE MASS - The present invention is based on the finding that enrichment of isotope-labeled creatinine in a urine sample following oral administration of a single defined dose of isotope-labeled can be used to calculate total-body creatine pool size and total body skeletal muscle mass in a subject. The invention further encompasses methods for detecting creatinine and isotope-labeled creatinine in a single sample. The methods of the invention find use, inter alia, in diagnosing disorders related to skeletal muscle mass, and in screening potential therapeutic agents to determine their effects on muscle mass. | 05-19-2016 |
20160139158 | FLUOROGENIC PH SENSITIVE DYES AND THEIR METHOD OF USE - A new class of pH sensitive fluorescent dyes and assays relating thereto are described. The dyes and assays are particularly suited for biological applications including phagocytosis and monitoring intracellular processes. The pH sensitive fluorescent dyes of the present invention include compounds of Formula I: | 05-19-2016 |
20160146778 | BIOPOLYMER SEPARATION USING NANOSTRUCTURED ARRAYS - A technique relates sorting biopolymers. The biopolymers are introduced into a nanopillar array, and the biopolymers include a first population and a second population. The nanopillar array includes nanopillars arranged to have a gap separating one from another. The biopolymers are sorted through the nanopillar array by transporting the first population of the biopolymers less than a predetermined bumping size according to a fluid flow direction and by transporting the second population of the biopolymers at least the predetermined bumping size according to a bumped direction different from the fluid flow direction. The nanopillar array is configured to employ the gap with a gap size less than 300 nanometers in order to sort the biopolymers. | 05-26-2016 |
20160154003 | BIOMARKERS AND METHODS FOR PREDICTING PRETERM BIRTH | 06-02-2016 |
20160154006 | Absolute Quantitation of Proteins and Protein Modifications by Mass Spectrometry with Multiplexed Internal Standards | 06-02-2016 |
20160161398 | Spatial Positioning of Spectrally Labeled Beads - Devices, systems, kits, and methods for detecting and/or identifying a plurality of spectrally labeled bodies well-suited for performing multiplexed assays. By spectrally labeling the beads with materials which generate identifiable spectra, a plurality of beads may be identified within the fluid. Reading of the beads is facilitated by restraining the beads in arrays, and/or using a focused laser. | 06-09-2016 |
20160161493 | Compositions, Methods and Kits for Diagnosis of Lung Cancer - The present invention provides methods for identifying biomarker proteins that exhibit differential expression in subjects with a first lung condition versus healthy subjects or subjects with a second lung condition. The present invention also provides compositions comprising these biomarker proteins and methods of using these biomarker proteins or panels thereof to diagnose, classify, and monitor various lung conditions. The methods and compositions provided herein may be used to diagnose or classify a subject as having lung cancer or a non-cancerous condition, and to distinguish between different types of cancer (e.g., malignant versus benign, SCLC versus NSCLC). | 06-09-2016 |
20160161503 | DIAGNOSTIC METHOD - The invention relates to a method of diagnosing or assessing an inflammatory bowel disease in a subject, comprising comparing the level of one or more markers in a tissue or body fluid of the subject relative to a reference value for the one or more markers, wherein the marker is selected from the group consisting of Secretogranin-1 or a fragment thereof, guanylin or a fragment thereof, SPP 24 or a fragment thereof, AMBP or a fragment thereof; and serglycin or a fragment thereof. | 06-09-2016 |
20160167054 | DEVICE FOR SIMULTANEOUS AND UNIFORM THERMAL CYCLING OF SAMPLES AND USES THEREOF | 06-16-2016 |
20160169862 | Method For Investigation Of Liver Damage Type | 06-16-2016 |
20160169863 | Means and Methods for Assessing Increased Peroxisomal Proliferation | 06-16-2016 |
20160169914 | KINETICS BIOMARKERS FOR NEURODEGENERATION | 06-16-2016 |
20160175801 | GENOMIC-SCALED NUCLEIC ACID SYNTHESIS, AND OTHER COMBINATORIAL SYNTHESES | 06-23-2016 |
20160178567 | Single-Walled Carbon Nanotube Biosensor for Detection of Glucose, Lactate, and Urea | 06-23-2016 |
20160178571 | COMPOSITIONS, KITS, AND DEVICES FOR GENERATING AND ACCURATELY MEASURING DIFFERENT OXYGEN LEVELS IN CALIBRATION AND/OR QUALITY CONTROL REAGENTS AND METHODS OF PRODUCTION AND USE THEREOF | 06-23-2016 |
20160178584 | METHODS FOR DIAGNOSING AND ASSESSING KIDNEY DISEASE | 06-23-2016 |
20160178646 | METHODS FOR DETECTING AMYLOID BETA AMYLOIDOSIS | 06-23-2016 |
20160186235 | APPARATUS FOR HIGH THROUGHPUT CHEMICAL REACTIONS - Apparatus, systems, chips, and methods of performing a large number of simultaneous chemical reactions are provided herein. The chips of the invention comprise addressable units that can be addressed according to the temperature of the reaction to be run. The subject apparatus, systems, and chips are particularly suited for performing polymerase chain reactions on thousands of nucleic acid sequences, up to and including sequences of an entire genome of an organism of interest. | 06-30-2016 |
20160187350 | CLEAVABLE PROBES FOR ISOTOPE TARGETED GLYCOPROTEOMICS AND METHODS OF USING THE SAME - Methods for producing isotopically-labelled peptides are provided. Aspects of the method include: contacting a sample including a metabolically tagged protein with a cleavable probe to produce a probe-protein conjugate; separating the probe-protein conjugate from the sample; digesting the probe-protein conjugate to produce a probe-peptide conjugate; and cleaving a cleavable linker to release an isotopically labelled peptide. The method may further include: identifying a predetermined isotopic pattern in a mass spectrum; determining an amino acid sequence of the isotopically labelled peptide; and identifying the site of protein glycosylation based on the determined amino acid sequence. Also provided are cleavable probes for practicing the subject methods, described by the Formula: A-L-(M-Z) where A is an affinity tag, L is a cleavable linker, M is an isotopic label and Z is a chemoselective tag capable of cross-linking a metabolically tagged protein. Compositions and kits for practicing the subject methods are also provided. | 06-30-2016 |
20160187351 | METHOD FOR THE ABSOLUTE QUANTIFICATION OF NATURALLY PROCESSED HLA-RESTRICTED CANCER PEPTIDES - The present invention relates to a method for the absolute quantification of naturally processed HLA-restricted cancer peptides, i.e. the determination of the copy number of peptide(s) as presented per cell. The present invention can not only be used for the development of antibody therapies or peptide vaccines, but is also highly valuable for a molecularly defined immuno-monitoring, and useful in the processes of identifying of new peptide antigens for immunotherapeutic strategies, such as respective vaccines, antibody-based therapies or adoptive T-cell transfer approaches in cancer, infectious and/or autoimmune diseases. | 06-30-2016 |
20160194590 | STRUCTURED BIOLOGICAL SAMPLES FOR ANALYSIS BY MASS CYTOMETRY | 07-07-2016 |
20160194685 | OPTICAL LENS SYSTEM AND METHOD FOR MICROFLUIDIC DEVICES | 07-07-2016 |
20160202238 | Method and apparatus for detecting elution of samples | 07-14-2016 |
20160252516 | SYSTEM AND METHOD FOR HIGH THROUGHPUT MASS SPECTROMETRIC ANALYSIS OF PROTEOME SAMPLES | 09-01-2016 |
20160377622 | METHODS FOR DIAGNOSING A COLORECTAL CANCER (CRC) HEALTH STATE OR CHANGE IN CRC HEALTH STATE, OR FOR DIAGNOSING RISK OF DEVELOPING CRC OR THE PRESENCE OF CRC IN A SUBJECT - The present invention relates to the diagnosis of colorectal and ovarian cancers (CRC and OC, respectively). The present invention describes the relationship between endogenous small molecules and CRC or OC. Specifically, the present invention relates to the diagnosis of CRC and OC through the measurement of vitamin E isoforms and related metabolites. The present invention also relates to diagnostic markers identified in said method. The present invention relates to the underlying case and pre-symptomatic phases of CRC, the diagnosis of various stages and severity of CRC, the early detection of CRC, monitoring and diagnosing the effect of therapy on CRC and OC health states. | 12-29-2016 |
20190144934 | NON-DESTRUCTIVE BILAYER MONITORING USING MEASUREMENT OF BILAYER RESPONSE TO ELECTRICAL STIMULUS | 05-16-2019 |