| Entries |
| Document | Title | Date |
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| 20090029874 | FLUORESCENCE BASED BIOSENSOR - A novel biosensor comprises at least one fluorophore and at least two quenchers, and is capable of selectively and specifically detecting the presence of an ion in the presence of other ions. | 01-29-2009 |
| 20090042738 | TYROSYLPROTEIN SULFOTRANSFERASES AND METHODS OF USE THEREOF - Tyrosylprotein sulfotransferases and nucleic acids encoding the tyrosylprotein sulfotransferases are described. Dual isotopes of the enzyme and of the nucleic acids encoding said enzymes have been identified in human, mouse and | 02-12-2009 |
| 20090054260 | Neoplasia Screening Compositions and Methods of Use - As described in more detail below, the present invention generally features compositions and non-invasive methods useful for the screening, identification, monitoring, or diagnosis of subjects having a neoplasia. The invention further provides highly accurate non-invasive methods for the staging or selection of treatment for a bladder, renal, or prostate cancer in a subject. | 02-26-2009 |
| 20090088340 | METHODS FOR PREDICTING THE REDUCTION/OXIDATION (REDOX) REACTION ACTIVITY OF METAL COMPLEXES - The presently disclosed subject matter relates to methods of predicting or measuring the reduction/oxidation (redox) reaction-related reactivity of a metal complex, particularly with respect to the ability of the metal complex to catalyze or inhibit the generation of reactive oxygen species (ROS) in vivo. The presently disclosed subject matter further relates to methods of screening and/or developing drug candidates that can mediate metal complex-catalyzed ROS generation. More particularly, the presently disclosed methods involve the use of probes having NMR active nuclei that can interact with paramagnetic metals in ways that can be easily detected by nuclear magnetic resonance (NMR) spectroscopy. | 04-02-2009 |
| 20090118139 | Microfluidic method and system for enzyme inhibition activity screening - Methods for screening a compound for enzyme inhibition activity include providing at least one sample mixture to a microfluidic device, applying vacuum pressure to the sample mixture, flowing the sample mixture along a microchannel of the microfluidic device, separating at least two components of the sample mixture based upon a net charge difference between the product and at least one other material to produce separated material, detecting at least one of the separated materials, and determining enzyme inhibition activity based on the detection of the separated material. Kits for screening a compound for enzyme inhibition activity include a first multiwell plate having a specific plurality of enzymes disposed within a first plurality of wells and a second multiwell plate having a plurality of enzyme substrates disposed with a second plurality of wells, a phosphate source and a cofactor disposed within each well of the second plate. | 05-07-2009 |
| 20090124515 | METHODS AND COMPOSITIONS FOR PREPARATION OF SELECTIVELY PROTECTED CARBOHYDRATES - The disclosure relates to engineered P450 polypeptides and use of such polypeptides in chemoenzymatic methods to construct selectively protected carbohydrates, which are useful as building blocks for preparation of carbohydrate derivatives and oligosaccharides | 05-14-2009 |
| 20090156426 | FUNCTIONALIZED POROUS SUPPORTS FOR MICROARRAYS - The present invention relates to functionalized porous carriers which comprise a material having at least one porous surface, nanoparticles having molecule-specific recognition sites being present in the pores of the material surface, and to a process for producing functionalized porous carriers. The invention further relates to functional elements produced using the functionalized carriers, such as microtiter plates, microarrays and flow devices, and also to uses of the functionalized carriers and functional elements. | 06-18-2009 |
| 20090203543 | Method for Assaying Enzyme Activity in Histological Sample - The present invention relates to methods for correlating histology and enzymatic activity of a biological sample, the method comprising the steps of: (i) providing a cryopreserved biological sample, wherein said sample comprises a series of consecutively sliced sections; (ii) obtaining histological data of a first subset of said series of sections; (iii) lysing a second subset of sections of said series of sections to obtain a lysate wherein specific enzyme activities are preserved; (iv) assaying the lysate of step (iii) for enzymatic reaction with one or more substrates; and (v) correlating the histology and enzymatic activity of the biological sample. | 08-13-2009 |
| 20090264311 | Thermostable peroxide-driven cytochrome P450 oxygenase variants and methods of use - The invention relates to novel variants of cytochrome P450 oxygenases. These variants have at least one mutation improving their ability to use peroxide as an oxygen donor as compared to the corresponding wild-type enzyme. The variants also have at least one mutation improving thermostability as compared to the parent enzyme or corresponding wild-type enzyme. Preferred variants include cytochrome P450 BM-3 heme domain variants having L52I, I58V, F87A, H100R, S106R, F107L, A135S, M145A/V, A184V, N239H, S274T, L324I, V340M, I366V, K434E, E442K, and/or V446I amino acid substitutions. | 10-22-2009 |
| 20100029507 | THE BIOCHIP FOR THE DETECTION OF PHOSPHORYLATION AND THE DETECTION METHOD USING THE SAME - The present invention relates to a biochip for the detection of phosphorylation and a method for measuring phosphorylation using the same, more precisely a biochip integrated with the substrate of kinase and a kit for measuring phosphorylation comprising the biochip and a radio-labeled co-factor, and a method for measuring phosphorylation using the same. The kit for the detection of phosphorylation of the present invention facilitates simple and fast measurement of phosphorylation with a minimum amount of a sample, compared with the conventional method using an antibody, because it uses a radioisotope. This chip and kit can be effectively used for the analysis of kinase activity since this method favors fast mass analysis. | 02-04-2010 |
| 20100160180 | Crystal Structure of an Angiotensin-Converting Enzyme (ACE) and Uses Thereof - The present invention relates to a crystal of ACE protein. The present invention further relates to methods, processes, ACE modulators, pharmaceutical compositions and uses of the ACE crystal and the structure co-ordinates thereof. | 06-24-2010 |
| 20100292100 | NOVEL DIARYLPHOSPHINE-CONTAINING COMPOUNDS, PROCESSES OF PREPARING SAME AND USES THEREOF AS TRIDENTATE LIGANDS - A novel process of preparing tridentate ligands containing a diarylphosphine electron donating group are disclosed. Use of this process for preparing a combinatorial library of such tridentate ligands and of organometallic complexes containing same is also disclosed. Further disclosed are novel diarylphosphine-containing compounds that can serve as tridentate ligands (e.g., pincer ligands), combinatorial libraries of such tridentate ligands, organometallic complexes containing these ligands (e.g., pincer complexes), and combinatorial libraries of such complexes. Methods utilizing these libraries for screening for candidate organometallic catalysts are also disclosed. | 11-18-2010 |
| 20100311611 | SYSTEMS FOR AND METHODS OF CHARACTERIZING REACTIONS - An automated and computerized system for characterizing kinetic activities is disclosed. The system includes an optical unit with a controller chip. The controller chip has multiple reaction cells for simultaneously reacting samples of the catalyst under a range of reaction conditions and for optically monitoring the kinetic activity within each of the reaction cells: The system also preferably includes a temperature controller in thermal contact with the controller chip and an actuation device coupled to the controller chip for injecting and mixing samples of the catalyst with reagents into each of the reaction cells to form a product. | 12-09-2010 |
| 20100317544 | Methods, Particles, and Kits for Determining Activity of a Kinase - Methods, particles and kits for determining kinase activity within a sample are provided. An embodiment of a method includes exposing a fluorescent particle to an assay, wherein the fluorescent particle includes a support substrate having one or more fluorescent materials and a peptide substrate coupled to the support substrate via a functional group of the support substrate. The method further includes phosphorylating the peptide substrate during exposure of the fluorescent particle to the assay and processing the fluorescent particle such that the peptide substrate is dephosphorylated and a polarized double bond is generated at a dephosphorylated site. In addition, the method includes coupling a fluorescent reporter having a nucleophilic terminal group to the fluorescent particle via the polarized double bond. | 12-16-2010 |
| 20110046012 | METHOD OF ENHANCING ENZYME ACTIVITY - Described are methods and means for enhancing enzyme activity toward insoluble substrates. This is achieved by means of in vitro compartmentalization in which an insoluble microparticle functions both as the enzyme substrate and as a structure for negative selection. Enhanced enzymes expressed from a microparticle-linked polynucleotide library preferentially degrade the microparticle releasing specific gene variants into solution. Gene variants encoding less active enzyme variants remain linked to the microparticle and may be removed through centrifugation, thus enriching the polynucleotide library for more active enzyme variants. These methods may be used to enhance cellulase and ligninase activity toward insoluble cellulosic biomass. | 02-24-2011 |
| 20110053797 | DEVICE AND METHOD FOR HANDLING MULTI-COMPONENT MIXTURE - Device and method for handling multi-component mixtures, comprising at least two not completely mixable fluid phases. The device comprises an arrangement of at least two high-pressure separators, being operatively connected each with a low-pressure separator. Each high-pressure separator comprises an outlet line for volatile gaseous constituents. Each low-pressure separator is operatively connected to a gas collecting container or shares at least one gas collecting container with a further low-pressure separator. At least one high-pressure separator has at least one outlet connection for gaseous constituents, being connected with a part to the analysis. The device is used in high-throughput research or in combinatory chemistry, preferably for the research of multi-component mixtures produced in experiments with catalyst, or in connection with high-pressure processes. | 03-03-2011 |
| 20110059863 | IN VIVO EVOLUTION OF HYDROGENASES USING A HYDROGEN-SENSING SYSTEM - Provided herein are methods for measuring H2 production by a hydrogenase. Also provided are methods for evolving a hydrogenase or hydrogen-sensing system by comparing the level of production of H2 by the hydrogenase to the level Of H2 produced by a wild-type hydrogenase cultured under the same conditions and selecting a microorganism with increased H2 production over the H2 production by a microorganism having a wild-type hydrogenase. Further provided herein are microorganisms and plasmids comprising a hupSL promoter coupled with a reporter gene. | 03-10-2011 |
| 20110065606 | PRODUCTION OF NUCLEIC ACID - The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex. | 03-17-2011 |
| 20110071052 | MEASUREMENT OF PROTEIN KINASE ACTIVITY IN CEREBROSPINAL FLUID FOR DIAGNOSIS OF NEUROLOGICAL AND PSYCHIATRIC DISORDERS - The present invention relates to the use of endogenous protein kinase activity in cerebrospinal fluid for the classification, diagnosis and prognosis of neurological and psychiatric disorders as well as for predicting and monitoring treatment effects. An array of substrates for protein kinases, immobilized on a porous matrix, is used to monitor the protein kinase activity in cerebrospinal fluid. The method of the present invention enables the early diagnosis and discrimination between neurodegenerative disorders. | 03-24-2011 |
| 20110082051 | SUBSTRATES, SYSTEMS AND METHODS FOR ANALYZING MATERIALS - Substrates, systems and methods for analyzing materials that include waveguide arrays disposed upon or within the substrate such that evanescent fields emanating from the waveguides illuminate materials disposed upon or proximal to the surface of the substrate, permitting analysis of such materials. The substrates, systems and methods are used in a variety of analytical operations, including, inter alia, nucleic acid analysis, including hybridization and sequencing analyses, cellular analyses and other molecular analyses. | 04-07-2011 |
| 20110086779 | ARRAYS OF PROTEIN CAPTURE AGENTS AND METHODS OF USE THEREOF - Arrays of protein-capture agents useful for the simultaneous detection of a plurality of proteins which are the expression products, or fragments thereof, of a cell or population of cells in an organism are provided. A variety of antibody arrays, in particular, are described. Methods of both making and using the arrays of protein-capture agents are also disclosed. The invention arrays are particularly useful for various proteomics applications including assessing patterns of protein expression and modification in cells. | 04-14-2011 |
| 20110118144 | ENGINEERED PHAGE VECTORS FOR THE DESIGN AND THE GENERATION OF A HUMAN NON-ANTIBODY PEPTIDE OR PROTEIN PHAGE LIBRARY VIA FUSION TO PIX OF M13 PHAGE - The invention relates to a compositions and methods for generating and using pIX phage display libraries for producing non-antibody peptide or protein proteins or peptides using engineered hybrid phage vectors derived from pIX of M 13 phage. | 05-19-2011 |
| 20110152124 | ELECTRICAL AND REUSABLE DEVICE FOR READING MICROARRAYS - The invention relates to a device for electrically reading microarrays which can be cleaned and used more than once. The device ( | 06-23-2011 |
| 20110166039 | Methods of Discovering or Developing Novel Materials and Molecules - The present inventions relates to methods of discovering or developing novel material or compound. In particular, the methods include the steps of using of general expert knowledge to identify currently available molecules, setting forth desired properties for a target molecule, designing a set of test molecule from the currently available molecule using computational methods (the test molecules having desired properties in the computational models); synthesizing the test molecules, testing the test molecules in real experiments, and identifying the target molecule which is the test molecule that has the desired properties. | 07-07-2011 |
| 20110190165 | HIGH THROUGHPUT METHODS OF IDENTIFYING NEUTRAL LIPID SYNTHASES - The present invention relates to high throughput methods of identifying neutral lipid synthases. The invention includes a method of positively selecting yeast cells expressing recombinant neutral lipid synthases, and quantifying the enzyme activities of the recombinant neutral lipid synthases using a fluorescence in situ assay. | 08-04-2011 |
| 20110224096 | FLUORESCENT PHOSPHONIC ESTER LIBRARY - An assay library comprising 14 fluorescently labeled phosphonate esters which act as serine hydrolase inhibitors is provided as an analytical tool enabling activity based identification of serine hydrolases and characterization of enzyme preparations, protein mixtures and complex proteome samples. | 09-15-2011 |
| 20110230370 | Discovery And Applications Of The Proteolytic Function of N-Terminal Acetylation Of Cellular Proteins - Screening assays that allow for the identification of agents that modulate the activity of N-terminal acetylation of a polypeptide and the Doa10 branch of the N-end rule pathway are provided. Also provided are methods of using an agent that modulates the activity of N-terminal acetylation of a polypeptide and the Doa10 branch of the N-end rule pathway to increase or decrease protein degradation in a cell, and to modulate physiologic and pathologic associated with N-terminal acetylation of a polypeptide and the Doa10 branch of the N-end rule pathway. | 09-22-2011 |
| 20110281763 | Apparatus and methods for high-throughput analysis - Disclosed is a high-throughput analysis apparatus. The high-throughput analysis apparatus comprises a sample introduction unit, a flow control unit, a separation unit, a detection unit, a signal collecting unit and a signal processing unit. Several methods using the same are also provided. | 11-17-2011 |
| 20110319293 | SYNTHETIC PROTEASE SUBSTRATES, ASSAY METHODS USING SUCH SUBSTRATES AND KITS FOR PRACTICING THE ASSAY - Synthetic protease substrates and methods which facilitate the identification of substrates of a protease, particularly ubiquitin, ubiquitin-like, or proteasome protein are provided. | 12-29-2011 |
| 20110319294 | STABLE, FUNCTIONAL CHIMERIC CELLOBIOHYDROLASE CLASS I ENZYMES - The present disclosure relates to CBH I chimera fusion polypeptides, nucleic acids encoding the polypeptides, and host cells for producing the polypeptides. | 12-29-2011 |
| 20120021950 | Expression Vector - An expression vector including two separately inducible converging promoters P1 and P2, and expression system including such an expression vector and an additional regulator vector, a method of protein expression using such an expression system, and a method of investigating (meta)genome libraries using such an expression system. | 01-26-2012 |
| 20120046199 | METHOD FOR DETERMINING THE ESTROGEN RECEPTOR STATUS OF BREAST CANCER - The present invention relates to a method for determining the estrogen receptor status of patients suffering from breast cancer. The present invention also aims to provide methods and devices for predicting the response of patients diagnosed with breast cancer to specific medicaments. More specifically, the present invention provides methods which measure kinase activity by studying phosphorylation levels and profiles in samples obtained from patients diagnosed with breast cancer. | 02-23-2012 |
| 20120065103 | Prolyl Endopeptidase Probes - Prolyl endopeptidase (PE) activity in lung samples is detected by contacting the lung sample with a probe comprising a —P—X— (or —X—P—, —P—X—P—) PE recognition site, wherein P is a prolyl bioisostere, X is a residue that is not a prolyl bioisostere or is a prolyl bioisostere flanked on each side by a residue that is not a prolyl bioisostere, and “-” is an amide bond, under conditions wherein PE activity of the sample specifically hydrolyzes an amide bond of the recognition site to generate an optical signal; and (b) detecting the signal. | 03-15-2012 |
| 20120077706 | METHOD FOR ASSAYING PROTEIN-PROTEIN INTERACTION - The invention relates to a method for determining if a test compound, or a mix of compounds, modulates the interaction between two proteins of interest. The determination is made possible via the use of two recombinant molecules, one of which contains the first protein a cleavage site for a proteolytic molecules, and an activator of a gene. The second recombinant molecule includes the second protein and the proteolytic molecule. If the test compound binds to the first protein, a reaction is initiated whereby the activator is cleaved, and activates a reporter gene. | 03-29-2012 |
| 20120083426 | METHOD - A method is described for releasing a soluble or membrane associated intracellular protein of interest (POI) comprising the steps of: providing a cell comprising a soluble or membrane associated intracellular POI; contacting the cell with a membrane extracting composition; and causing the POI to be released from the cell under conditions sufficient for the specific release of the POI and in a soluble form. | 04-05-2012 |
| 20120083427 | KINASE AND PHOSPHATASE ASSAYS - Compositions, methods, and kits for detecting and monitoring kinase, phosphatase and protein post-translational modification activity are described. The compositions typically include a peptide, a detectable moiety, and a protease cleavage site. Modification of a peptide by a kinase, phosphatase or other protein post-translational modification alters the proteolytic sensitivity of the peptide, resulting in a change of a detectable property of the composition. Panel assays for determining substrates or modulators of kinase, phosphatase or other protein post-translational modification activity are also described. | 04-05-2012 |
| 20120115754 | METHOD FOR DIAGNOSING AND/OR TYPING RENAL CELL CARCINOMA - The present invention relates to a method for diagnosing, typing and/or subtyping renal cell carcinoma as well as predicting the response to medication of patients suffering from renal cell carcinoma. More specifically, the present invention provides methods which measure kinase activity by studying phosphorylation levels and profiles in samples of said patients. | 05-10-2012 |
| 20120122730 | Methods for Quantitative Analyses of Kinase Inhibitor Selectivity Using Small Size Panels - Methods for analyses of kinase inhibitor specificity and promiscuity using small subsets of kinases including a method comprising providing a set of kinases, ranking the kinases based upon their ability to overcome biases, utilizing a correlation-based feature selection algorithm to select a kinase inferential bases, and screening a kinase inhibitor against the kinase inferential bases. | 05-17-2012 |
| 20120135888 | METHOD FOR PREDICTING THE RESPONSE OF LOCALLY ADVANCED RECTAL CANCER TO CHEMORADIOTHERAPY - The present invention relates to a method for determining or predicting the response of a patient diagnosed with locally advanced rectal cancer to chemoradiotherapy. The present invention also aims to provide methods and devices for predicting the response of patients diagnosed with rectal cancer to specific medicaments, radiotherapy and/or chemotherapy. More specifically, the present invention provides methods which measure kinase activity by studying phosphorylation levels and profiles in samples of said patients. | 05-31-2012 |
| 20120157345 | Methods and Compositions for Obtaining and Using Biologically Active Multi-Protein Complexes - Methods for isolating and using multi-protein complexes that are biologically active are provided. The complexes contain one or more proteins of interest (e.g. a receptor, ion channel, etc.) and associates scaffolding proteins such as phosphatases, kinases and post synaptic density components. Buffers that do not contain denaturing agents and which may be used to isolate the multi-protein complexes are also provided, as are protein arrays containing the biologically active multi-protein complexes. The protein arrays may be used, for example, for high throughput screening assays. | 06-21-2012 |
| 20120220490 | METHOD AND COMPOSITIONS FOR DETECTING BOTULINUM NEUROTOXIN - The present invention provides a molecular construct capable of fluorescent resonance energy transfer (FRET), comprising a linker peptide, and donor and acceptor fluorophore moieties, where the linker peptide is a substrate of a botulinum neurotoxin selected from the group consisting of synaptobrevin, syntaxin and SNAP-25, or a fragment thereof capable being cleaved by the botulinum neurotoxin, and separates the donor and acceptor fluorophores by a distance of not more than 10 nm, and where emission spectrum of the donor fluorophore moiety overlaps with the excitation spectrum of the acceptor fluorophore moiety; or where the emission spectra of the fluorophores are detectably different. Also provided are isolated nucleic acid expressing the construct, kits comprising said construct and cell lines comprising said nucleic acid. Further provided are methods of detecting a BoNT using the above described construct via FRET, and methods for detecting a BoNT using surface plasmon resonance imaging. | 08-30-2012 |
| 20120225797 | MULTIPLEXED SCREENING OF ENZYME ACTIVITIES USING NANOSTRUCTURE-INITIATOR MASS SPECTROMETRY - Disclosed herein are methods, compositions and systems for analyzing and detecting enzyme activity. For examples, methods, compositions and systems for parallel detection and analysis of enzymatic activities of enzymes in complex biological mixtures are provided. | 09-06-2012 |
| 20120231972 | Probe Compound for Detecting and Isolating Enzymes and Means and Methods Using the Same - The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing. | 09-13-2012 |
| 20120238470 | METHOD FOR SCREENING AND QUANTIFYING VARIOUS ENZYME ACTIVITIES USING A GENETIC ENZYME SCREENING SYSTEM - A method of detecting and quantifying various enzymatic activities using a constructed artificial genetic circuit GESS (genetic enzyme screening system) for sensing phenolic compounds and a method of screening a trace of activities of target enzymes from a metagenome using the artificial genetic circuit, thereby securing target enzyme genes. When the method for screening and quantifying target enzymatic activity is used, useful genes can be screened from various genetic communities, including environmental or metagenomic libraries, at a single cell level in high throughput (million/day). Further, the sensitivity of the genetic circuit to phenol derivatives and the expression thereof can be controlled, and thus the genetic circuit can rapidly sense and quantify various enzymatic activities. Thus, the method can be advantageously used in the protein engineering technology for enzyme modification. Particularly, it can quantitatively investigate enzymatic activity, and thus can be applied to molecular evolution technology. | 09-20-2012 |
| 20120252698 | ISOLATED AUSTRALIAN CORAL REEF FLUORESCENT PROTEINS AND CELL-BASED KINASE OR PHOSPHATASE PLATFORMS FOR CANCER DRUG DEVELOPMENT - The present invention concerns novel isolated fluorescent proteins, variants thereof, and polynucleotides encoding the same. Methods for making and using the polypeptides and polynucleotides are also provided. For example, methods to detect protein-protein interactions, to develop novel fluorescent reagents, to monitor cellular events, as well as cell-based methods for screening for kinase or phosphatase inhibitors, are set forth. Kits to carry out the methods of the invention are also taught. | 10-04-2012 |
| 20120264646 | ENZYME QUANTIFICATION - The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number. | 10-18-2012 |
| 20120309648 | INTEGRATED MICROFLUIDICS FOR HIGHLY PARALLEL SCREENING OF CHEMICAL REACTIONS - A microfluidic system has a microfluidic mixer and a sample storage component that is in fluid connection with the microfluidic mixer. The microfluidic mixer has a mixing section; a target molecule input section that is in fluid connection with the mixing section, the target molecule input section being suitable to provide a fluid into the mixing section that contains molecules to be targeted by chemical reactions; a first reagent input section that is in fluid connection with the mixing section, the first reagent input section being structured to selectively provide a first reagent selected from a plurality of reagents to test a chemical reaction with the target molecules; a second reagent input section that is in fluid connection with the mixing section, the second reagent input section being structured to selectively provide a second reagent selected from a plurality of reagents to test a chemical reaction with the target molecules and said first reagent; and a neutral fluid input section that is in selectable fluid connection with the sample storage component, the neutral fluid input section being structured to selectively provide a neutral fluid into the sample storage component between successive samples provided to the sample storage component to separate successive samples in a stratified arrangement. | 12-06-2012 |
| 20120322689 | USE OF MEGANUCLEASES FOR INDUCING HOMOLOGOUS RECOMBINATION EX VIVO AND IN TOTO IN VERTEBRATE SOMATIC TISSUES AND APPLICATION THEREOF - The present invention provides a method for detecting nucleic acid cleavage induced by a modified rare-cutting endonuclease derived from an initial rare-cutting endonuclease, said modified rare-cutting endonuclease being able to cleave a DNA target sequence, which may be different from the recognition and cleavage site of the initial rare-cutting endonuclease. In one embodiment of the invention expression of a marker gene is used as an indicator of target DNA cleavage. | 12-20-2012 |
| 20130029877 | METHODS FOR OPTIMIZING OPTICAL MAPPING CONDITIONS - The invention generally relates to methods and apparatuses for optimizing conditions for optical mapping. In certain embodiments, methods of the invention involve providing a substrate including a gradient of silanes in a first direction, introducing to the substrate, a gradient of enzyme activity in a second direction, contacting a plurality of enzymes and a plurality of nucleic acids to the substrate, and analyzing enzymatic activity and interaction of the nucleic acids with the substrate, thereby determining the optimal conditions for optical mapping of the nucleic acid. | 01-31-2013 |
| 20130029878 | Method for Assaying Histone Methylation Enzyme Activity - Provided are a method for measuring histone methyltransferase activity, a method for screening for compounds that inhibit histone methyltransferase activity, a reagent kit for measuring histone methyltransferase activity, and a kit for screening for compounds that inhibit histone methyltransferase activity. A substrate compound represented by general formula (I): R | 01-31-2013 |
| 20130035257 | MICROFLUIDIC ARRAYS AND METHODS FOR THEIR PREPARATION AND USE - Methods of isolating at least one cell of interest, methods of making fixed arrays, arrays comprising a glass substrate bonded to a patterned siloxane structure having inlets, outlets and microchannels, array kits, and methods of making microfluidic apparati are provided in the present application. | 02-07-2013 |
| 20130040856 | Generation of Combinatorial Patterns by Deliberate Tilting of a Polymer-Pen Array - The disclosure relates to a method of forming a pattern having pattern elements with a plurality of sizes on a substrate surface with a tilted pen array that includes choosing a tilt geometry for a pen array with respect to a substrate, inducing the tilt geometry between the pen array and the substrate surface, and forming a pattern having pattern elements on the substrate surface with the titled pen array, whereby the size of the formed pattern elements varies across the substrate surface along the tilted axis or axes. For example, the tilt geometry is in reference to the substrate surface and comprises a first angle with respect to a first axis of the substrate and a second angle with respect to a second axis of the substrate, the second axis being perpendicular to the first axis, and at least one of the first and second angles being non-zero. | 02-14-2013 |
| 20130072404 | APPARATUS AND PROCESSES FOR GENERATING VARIABLE CONCENTRATION OF SOLUTES IN MICRODROPLETS - The present invention relates to systems and methods for generating microdroplets with varying concentrations of a particular solute from a solution at fixed concentration. | 03-21-2013 |
| 20130102498 | COMPOSITIONS AND METHODS FOR DETECTING AMYLOID-BETA-DEGRADING ENZYME ACTIVITY - Novel substrates for detection of activity of amyloid beta degrading enzyme, such as Neprilysin (NEP) and insulin degrading enzyme (IDE), associated with Alzheimer's disease, are provided. A quenched fluorogenic peptide substrate containing the first seven residues of the Aβpeptide plus a C-terminal Cys residue to detect neprilysin activity with a fluorophore attached to the C-terminal Cys and a quencher linked to the N-terminus of the peptide is disclosed. An assay system sensitive to endopeptidase activity of NEP and IDE, but insensitive to other Aβ-degrading enzymes is disclosed. Active compounds are identified by a cell-based assay system for high-throughput screening. | 04-25-2013 |
| 20130102499 | METHOD FOR DETERMINATION OF ACTIVITY OF MITOCHONDRIAL DNA POLYMERASE OF FALCIPARUM MALARIA, AND METHOD FOR SCREENING FOR ANTI-MALARIA COMPOUND - An object is to provide a means which is useful for the development of an anti-malaria agent. It was found that a mitochondrial DNA polymerase of falciparum malaria shows a bivalent iron ion requirement. Thus, disclosed is a method for measuring the activity of a DNA polymerase, including the steps of: (1) incubating a solution containing a bivalent iron ion, a mitochondrial DNA polymerase of falciparum malaria, template DNA, and at least one deoxyribonucleoside triphosphate or deoxyribonucleoside triphosphate derivative; (2) detecting the synthesized double-stranded DNA; and (3) calculating the activity of the DNA polymerase from the result of the detection carried out in step (2). | 04-25-2013 |
| 20130116152 | UBIQUITINATION ASSAY - The present application relates to a method of assaying ubiquitination in a sample by combining ubiquitin together with a substrate in a sample containing UBE1, UbcH3, Skp2-isoform 1, Skp1, Cul1, Rbx1, Cks1, CDK2 and Cyclin E1 under conditions suitable for ubiquitination to take place, exposing the sample to a labelled binding partner which is specific for the ubiquitin, and measuring the amount of ubiquitin bound to the substrate. | 05-09-2013 |
| 20130123141 | Cellular Libraries of Peptide Sequences (CLiPS) and Methods of Using the Same - The present invention provides compositions including peptide display scaffolds that present at least one candidate peptide and at least one detectable moiety in at least one of the N-terminal and C-terminal candidate peptide presenting domains that when expressed in a cell are accessible at a surface of the cell outermembrane. In addition, the present invention also provides kits and methods for screening a library of cells presenting the candidate peptides in peptide display scaffolds to identify a ligand for an enzyme. | 05-16-2013 |
| 20130123142 | COMPOSITION FOR PREVENTING OR TREATING DIABETES COMPRISING NAD GLYCOHYDROLASE INHIBITOR AS ACTIVE INGREDIENT - The present invention provides a composition for preventing or treating diabetes, comprising an NAD glycohydrolase (NADase) inhibitor as an active ingredient. The NAD glycohydrolase (NADase) inhibitor according to the present invention has the effect of reducing blood glucose levels and promoting insulin secretion and thus can be effectively used as a therapeutic agent for diabetes, particularly type II diabetes. | 05-16-2013 |
| 20130123143 | METHOD FOR IDENTIFYING A COMPOUND HAVING AN ANTIARRHYTHMIC EFFECT AS WELL AS USES RELATING THERETO - The present invention relates to a method of identifying a compound having an antiarrhythmic effect and to a use of adenosine-diphospho-ribose cyclase (cardiac ADPRC) for the identification of a compound having said effect. | 05-16-2013 |
| 20130130935 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 05-23-2013 |
| 20130190209 | High Fidelity Restriction Endonucleases - Compositions and methods are provided for enzymes with altered properties that involve a systematic approach to mutagenesis and a screening assay that permits selection of the desired proteins. Embodiments of the method are particularly suited for modifying specific properties of restriction endonucleases such as star activity. The compositions includes restriction endonucleases with reduced star activity as defined by an overall fidelity index improvement factor. | 07-25-2013 |
| 20130217595 | METHOD FOR THE IDENTIFICATION OF CATECHOL O-METHYLTRANSFERASE MODULATORS - The present invention relates to a method for the identification of modulators of catechol O-methyltransferase enzyme activity (COMT). | 08-22-2013 |
| 20130231265 | TYROSINE KINASE BIOSENSORS AND METHODS OF USE - Disclosed are compositions and methods for measuring tyrosine kinase activity. | 09-05-2013 |
| 20130252849 | METHODS FOR SCREENING PROTEINS USING DNA ENCODED CHEMICAL LIBRARIES AS TEMPLATES FOR ENZYME CATALYSIS - Disclosed are methods, compositions and devices for screening a protein library for proteins having a desired activity, such as capable of catalyzing the formation of a bond between two reactants. In an exemplary embodiments, a plurality of proteins are expressed in vitro from a plurality of nucleic acids, the plurality of proteins are exposed with two single stranded oligonucleotides having complementary sequences, each oligonucleotide having a reactant and a fluorophore, the fluorescence of the protein-reactant-oligonucleotide-fluorophore complexes is detected and the complexes showing detectable fluorescence energy transfer are isolated, thereby isolating proteins having the desired enzymatic activity. | 09-26-2013 |
| 20130252850 | Alpha-Amylase Variants - The invention relates to a variant of a parent Termamyl-like alpha-amylase, comprising mutations in two, three, four, five or six regions/positions. The variants have increased stability at high temperatures (relative to the parent). The invention also relates to a DNA construct comprising a DNA sequence encoding an alpha-amylase variant of the invention, a recombinant expression vector which carries a DNA construct of the invention, a cell which is transformed with a DNA construct of the invention, the use of an alpha-amylase variant of the invention for washing and/or dishwashing, textile desizing, starch liquefaction, a detergent additive comprising an alpha-amylase variant of the invention, a manual or automatic dishwashing detergent composition comprising an alpha-amylase variant of the invention, a method for generating a variant of a parent Termamyl-like alpha-amylase, which variant exhibits increased. | 09-26-2013 |
| 20130288925 | PRODUCTION OF NUCLEIC ACID - A method for the production of nucleic acid encoding a target protein. The method comprises (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex. | 10-31-2013 |
| 20130296192 | Protein Arrays and Methods of Using and Making the Same - Methods and devices are provided for preparing a protein array having a plurality of proteins. In one embodiment, the method includes providing a plurality of nucleic acids each having a predefined sequence and expressing in vitro a plurality of proteins from the plurality of nucleic acids. In another embodiment, protein arrays having a solid surface and a microvolume are also provided. The solid surface can have a plurality of anchor oligonucleotides capable of hybridizing with a plurality of nucleic acids. The microvolume can cover each of the plurality of anchor oligonucleotides and can be configured to produce a polypeptide from each of the plurality of nucleic acids. | 11-07-2013 |
| 20140005072 | CATALYTIC NUCLEIC ACID PROBES AS MICROBIAL INDICATORS | 01-02-2014 |
| 20140011705 | DETECTION OF PROTEASE AND PROTEASE ACTIVITY USING A SINGLE NANOCRESCENT SERS PROBE - This invention pertains to the in vitro detection of proteases using a single peptide-conjugate nanocrescent surface enhanced Raman scattering (SERS) probes with at least nanomolar sensitivity. The probe enables detection of proteolytic activity in extremely small volume and at low concentration. In certain embodiments the probes comprise an indicator for the detection of an active protease, where the indicator comprises a nanocrescent attached to a peptide, where said peptide comprises a recognition site for the protease and a Raman tag attached to the peptide. | 01-09-2014 |
| 20140024558 | Method of Treatment - The present invention relates to methods for treatment or prevention of autoimmune and inflammatory diseases and conditions by inhibiting or modifying histone demethylation. In a further aspect the invention relates to a method for identifying agents useful in said methods of treatment. The invention particularly describes the role of certain histone demethylase enzymes in these diseases and conditions and their use as therapeutic and screening targets. | 01-23-2014 |
| 20140038850 | METHODS AND SYSTEMS FOR EVALUATING AND PREDICTING THE REACTIVITY OF MONOOXYGENASE ENZYMES - Methods and systems for evaluating and predicting the reactivity of natural and engineered monooxygenase enzymes are provided. Methods are provided for acquiring a functional profile (fingerprint) of monooxygenases that encode information regarding the active site configuration of such monooxygenases. Methods are also provided for carrying out analysis of a monooxygenase fingerprint, to formulate predictions regarding the reactivity properties (e.g., substrate reactivity, chemo-, regio, and stereoselectivity properties) of the fingerprinted monooxygenases. | 02-06-2014 |
| 20140045723 | Method of Enhancing Enzyme Activity - Described are methods and means for enhancing enzyme activity toward insoluble substrates. This is achieved by means of in vitro compartmentalization in which an insoluble microparticle functions both as the enzyme substrate and as a structure for negative selection. Enhanced enzymes expressed from a microparticle-linked polynucleotide library preferentially degrade the microparticle releasing specific gene variants into solution. Gene variants encoding less active enzyme variants remain linked to the microparticle and may be removed through centrifugation, thus enriching the polynucleotide library for more active enzyme variants. These methods may be used to enhance cellulase and ligninase activity toward insoluble cellulosic biomass. | 02-13-2014 |
| 20140135235 | GLYCAN AND GLYCOPEPTIDE CAPTURE AND RELEASE USING REVERSIBLE HYDRAZONE-BASED METHOD - Highly specific and novel methods for reversible hydrazone solid-phase extraction (rHSPE) are provided for glycan or glycopeptide isolation from proteins, peptides, and other contaminants for glycan and glycopeptide analysis. Glycans or glycopeptides in complex mixtures can be conjugated onto solid support or affinity or chemical tags via reversible hydrazone bond. The conjugation methods of the present invention are chemically specific and provide unique means for the removal of other non-glycan containing molecules in the complex sample before the glycans or glycopeptides are hydrolyzed and recovered for analysis. The hydrazone formation and hydrolysis of the novel methods allows for the analysis of glycans and glycopeptides. The hydrazide coating on the solid-phase surfaces are useful for surface glycan capture and on target glycan analysis. Uses of the information generated by the inventive methods for diagnosis and treatment are also disclosed. | 05-15-2014 |
| 20140148361 | Generation and Expression of Engineered I-ONUI Endonuclease and Its Homologues and Uses Thereof - The present disclosure provides compositions and methods for producing and expressing variant or engineered I-OnuI endonucleases, variant or engineered I-OnuI homologues, and hybrids of two I-OnuI or I-OnuI homologue domains that have a target site altered from the wild-type. A method for selecting a variant or engineered I-OnuI endonuclease, I-OnuI endonuclease homologue, and a hybrid of two I-OnuI or I-OnuI homologue domains that have a target site altered from the wild-type and directed to a site within a gene of interest is also provided. In addition, the present disclosure provides the crystal structure of the I-OnuI and I-LtrI endonucleases; the specificity profiles for both endonuclease for DNA binding and cleavage; the identity of amino acid residue positions in the I-OnuI and I-LtrI protein scaffold that determine DNA recognition specificity; methods for determining amino acid substitutions at those positions that alter DNA cleavage specificity; methods for the complete redesign of the DNA cleavage specificity of I-OnuI and its homologues for recognition and cleavage of a human gene of interest; and the relationship of the amino acid sequence, structure and specificity of I-OnuI to a collection of identifiable I-OnuI endonuclease homologues. | 05-29-2014 |
| 20140162902 | Cell-Based Materials and Methods for Defining Pharmacogenetic Differences in Drug Metabolism - Cell lines harboring a range of polymorphisms using recombinant cytochrome P450 or other chemical-metabolizing enzymes in a parent cell line that is minimally expressing or devoid of its own cytochrome P450 protein or other chemical-metabolizing enzymes of interest can be placed into an array format to enable high throughput screening of one or more chemicals for CYP450 or other enzyme-dependent metabolism (FIG. | 06-12-2014 |
| 20140235501 | Enzymatic Biosensing Systems - Biosensors and methods of producing biosensors for use in detecting one or more analytes in a solution are disclosed herein. | 08-21-2014 |
| 20140235502 | METHOD FOR SCREENING AND QUANTIFYING ISOPRENE BIOSYNTHESIS ENZYME ACTIVITY - A method of performing high-throughput screening of various enzymatic activities with high sensitivity using artificial genetic circuits is provided. Particularly, the invention is screening and quantifying the activity of isoprene biosynthesis enzymes using an artificial genetic circuit capable of sensing isoprene. The artificial genetic circuit comprises an isoprene-sensing transcriptional regulator which recognizes isoprene, at least one reporter gene, a isoprene-sensing transcriptional regulator binding region and promoters for genes encoding isoprene-sensing transcriptional regulators and reporter proteins. | 08-21-2014 |
| 20140243241 | METHOD FOR MEASURING BETA-LACTAM ANTIBIOTICS - The present invention relates to methods for determining the concentration of a free beta-lactam antibiotic in a biological sample. In particular, the present invention relates to methods for measuring a free beta-lactam antibiotic in a biological sample, comprising the steps of: (a) providing at least one class C beta-lactamase, a functional fragment or variant thereof; (b) providing at least one biological sample; (c) contacting said at least one class C beta-lactamase with said at least one biological sample; and (d) determining the concentration of said free beta-lactam antibiotic in said at least one biological sample. | 08-28-2014 |
| 20140249054 | LYSOSOMAL ENZYME ASSAY METHODS AND COMPOSITIONS - Multiplex enzyme assay methods and compositions for simultaneously assaying the activities of a plurality of lysosomal enzymes. | 09-04-2014 |
| 20140274797 | FRET BASED MULTIPLEX PROBES - The present invention includes compositions and methods for fluorescence-based multiplex probe to simultaneously detect one or more enzymatic activities comprising: a first enzymatic target having a first end and a second end, wherein the first end of the first enzymatic target is attached to a central body and the second end of the first enzymatic target is attached to a first fluorophore; a second enzymatic target having a first end and a second end, wherein the first end of the second enzymatic target is attached to the central body and the second end of the second enzymatic target is attached to a third fluorophore; wherein the central body comprises at least one second fluorophore; wherein the first enzymatic target comprises a specific cleavage site of a first enzyme that cleaves the first enzymatic target; and wherein the second enzymatic target comprises a specific cleavage site of a second enzyme. | 09-18-2014 |
| 20140274798 | COMPOUNDS AND METHODS RELATING TO LYSOSOMAL STORAGE DISORDERS - Substrates are provided that include compounds suitable for detecting the activity of an enzyme such as a lysosomal storage enzyme where the substrates include: a sugar moiety; a linker moiety allowing the conjugation of sugar moiety with the remaining structure of the substrate; and two or more fatty acid chains or derivatives thereof at least one of which is sufficiently structured to provide improved solubility in aqueous or organic solvent systems. Also provided are methods for using substrates for detecting enzymatic activity using the inventive substrates. | 09-18-2014 |
| 20140309141 | Methods of Screening for Inhibitors of Enzymes - Methods and compositions for detection of the modulators of proteolytic enzymes, particularly cysteine proteases, are disclosed. | 10-16-2014 |
| 20140336079 | LABELING AND DETECTION OF NUCLEIC ACIDS - Provided in certain embodiments are new methods for forming azido modified nucleic acid conjugates of reporter molecules, carrier molecules or solid support. In other embodiments are provided methods for enzymatically labeling nucleic acids with an azide group. | 11-13-2014 |
| 20140357523 | METHOD FOR FRAGMENTING GENOMIC DNA USING CAS9 - A method for fragmenting a genome is provided. In certain embodiments, the method comprises: (a) combining a genomic sample containing genomic DNA with a plurality of Cas9-gRNA complexes, wherein the Cas9-gRNA complexes comprise a Cas9 protein and a set of at least 10 Cas9-associated guide RNAs that are complementary to different, pre-defined, sites in a genome, to produce a reaction mixture; and (b) incubating the reaction mixture to produce at least 5 fragments of the genomic DNA. Also provided is a composition comprising at least 100 Cas9-associated guide RNAs that are each complementary to a different, pre-defined, site in a genome. Kits for performing the method are also provided. In addition, other methods, compositions and kits for manipulating nucleic acids are also provided. | 12-04-2014 |
| 20140357524 | Method for Quantifying Subject Substance - There is provided a method for quantifying a subject substance, of which typical examples are amino acids. The method of the present invention comprises the following steps: the step of allowing an enzyme that can generate pyrophosphate by using adenosine triphosphate (ATP) as a substrate with converting the subject substance to act on the subject substance to generate pyrophosphate; the step of allowing pyruvate pyrophosphate dikinase (PPDK) to act on the generated pyrophosphate in the presence of adenosine monophosphate (AMP) and phosphoenolpyruvate (PEP) to generate ATP, phosphoric acid, and pyruvate; and the step of quantifying the generated pyruvate, and amount of the subject substance is determined on the basis of the obtained amount of pyruvate. According to the present invention, an amino acid in a biological sample containing a lot of various kinds of contaminants such as inorganic phosphoric acid and urea can be conveniently and quickly quantified without being influenced by the contaminants. | 12-04-2014 |
| 20150011421 | ELECTROCHEMICAL DETECTION OF PROTEASES USING AC VOLTAMMETRY ON NANOELECTRODE ARRAYS - An electrochemical method for measuring the activity of enzymes using nanoelectrode arrays fabricated with vertically aligned carbon nanofibers. Short peptide substrates specific to disease-related enzymes are covalently attached to the exposed nanofiber tips. A redox moiety, such as ferrocene, can be linked at the distal end of the nanofibers. Contact of the arrays with a biological sample containing one or more target enzymes results in cleavage of the peptides and changes the redox signal of the redox moiety indicating the presence of the target enzymes. | 01-08-2015 |
| 20150011422 | METHODS TO ASSAY PHOSPHATASE ACTIVITY - Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enyzmes | 01-08-2015 |
| 20150038372 | METHODS, MICROFLUIDIC DEVICES, AND SYSTEMS FOR DETECTION OF AN ACTIVE ENZYMATIC AGENT - Embodiments of the present invention provide methods, microfluidic devices, and systems for the detection of an active target agent in a fluid sample. A substrate molecule is used that contains a sequence which may cleave in the presence of an active target agent. A SNAP25 sequence is described, for example, that may be cleaved in the presence of Botulinum Neurotoxin. The substrate molecule includes a reporter moiety. The substrate molecule is exposed to the sample, and resulting reaction products separated using electrophoretic separation. The elution time of the reporter moiety may be utilized to identify the presence or absence of the active target agent. | 02-05-2015 |
| 20150051110 | METHODS FOR IDENTIFYING MODULATORS OF RAS USING NONLINEAR TECHNIQUES - Provided herein are compositions and methods for identifying and detecting modulators of Ras protein conformational states through the use of second harmonic generation (SHG) technology. Also provided herein are methods for detecting a conformational changes in the three dimensional structure of a protein bound to a supported lipid bilayer. | 02-19-2015 |
| 20150057185 | REACTION-BASED FLUORESCENT PROBE FOR SELECTIVE DETECTION OF CARBON MONOXIDE USING METAL-MEDIATED CARBONYLATION - Carbon monoxide (CO) is a member of the gasotransmitter family that includes NO and H | 02-26-2015 |
| 20150072894 | CYANINE DYES - The invention provides a novel class of cyanine dyes that are functionalized with sulfonic acid groups and a linker moiety that facilitates their conjugation to other species and substituent groups which increase the water-solubility, and optimize the optical properties of the dyes. Also provided are conjugates of the dyes, methods of using the dyes and their conjugates and kits including the dyes and their conjugates. | 03-12-2015 |
| 20150111785 | Method for Simultaneously Determining Multiple Coagulation Proteases - The present invention relates to a chromogenic method for simultaneously determining the activity of multiple coagulation proteases or for simultaneously determining the inhibition of multiple coagulation proteases in a single test reaction. For this purpose, use is made of two chromogenic substrates which have different absorption maxima and whose color signals can be separated spectrally. | 04-23-2015 |
| 20150111786 | METHODS OF TREATING A MEIOTIC KINESIN-ASSOCIATED DISEASE - The invention provides methods of treating a meiotic kinase-associated disease, preferably the meiotic kinase HSET, by administering an inhibitor of the meiotic kinase. Preferably, the disease is associated with the presence of supernumerary centrosomes, such as cancer. Methods of inhibiting the growth of a tumor cell by contacting the cell with an inhibitor of a meiotic kinase, preferably HSET, are also provided. Screening methods for identifying inhibitors of the meiotic Kinase HSET are also provided. Methods of selecting subjects for treatment with an inhibitor of a meiotic kinase, such as HSET, are also provides. | 04-23-2015 |
| 20150290612 | ANALYTE SENSOR CHIPS - The sensor chips, processes and devices enable ultra-sensitive detection/determination, evaluation and quantitative measurement of analytes and are useful for high throughput and miniaturized assays, which enable a user to perform multiple, accurate, experiments in parallel with minimum amount of reagents resulting in low waste generation. The method enables screening of fluid samples to meet regulatory standards. The device comprises a pitted chip having a silicon-based substrate, optionally provided with an integrated heating element, a biosensor and a receptor immobilized on a cross linking element fixed to an inert metal layer in the chip. The analyte is detected up to 5 parts per trillion of the fluid sample and quantitatively measured up to 10 parts per trillion of the fluid sample by the device of the present disclosure. | 10-15-2015 |
| 20150298089 | Systems for Filling a Sample Array by Droplet Dragging - A method and an array filling system for loading a plurality of disparate sample containers, the sample containers comprising an integral structure. Each receptacle is characterized by a hydrophilic surface,, and the receptacles are separated by a hydrophobic surface. The system has a liquid transfer device capable of holding liquid and adapted for motion to cause sequential communication of liquid held in the liquid transfer device with successive receptacles of the array by dragging the liquid across the hydrophobic surface. | 10-22-2015 |
| 20150330992 | RAPID DISCOVERY AND SCREENING OF ENZYME ACTIVITY USING MASS SPECTROMETRY - Described herein are methods, systems, and compositions for detecting enzyme activity. In some embodiments, the reaction product(s) are coupled with a mass tag, and the enzyme activity is determiner by analyzing the reaction product(s). The enzyme assays can be performed using mass spectrometry, for example nanostructure-initiator mass spectrometry (NIMS). Also described are methods, systems, and compositions for monitoring enzymatic degradation process of a substrate sample, for example a biomass. | 11-19-2015 |
| 20150346203 | Methods and Compositions for Use of Neutrophil Elastase and Proteinase 3 as Diagnostic Biomarkers - Methods and compositions for identifying autoimmune diabetes are provided. One aspect provides a method for the evaluation of risk and progression of autoimmune diabetes in mammalian subjects. The method includes measuring the enzymatic activities and/or protein concentrations of neutrophil elastase and proteinase 3 in a subject and comparing the measured levels of these proteases to respective reference levels. | 12-03-2015 |
| 20150346204 | MEK Mutations Conferring Resistance to MEK Inhibitors - The present invention relates to methods, compositions and kits concerning resistance to treatment with an anti-cancer agent, specifically an inhibitor of MEK. In particular embodiments, the invention concerns mutations in a MEK sequence that confer resistance to a MEK inhibitor. Identification of such mutations in a MEK sequence allows the identification and design of second-generation MEK inhibitors. Methods and kits for detecting the presence of a mutant MEK sequence in a sample are also provided. | 12-03-2015 |
| 20160010084 | Cellular Libraries of Peptide Sequences (CLiPS) and Methods of Using the Same | 01-14-2016 |
| 20160061836 | METHODS RELATING TO TESTING FOR LYSOSOMAL STORAGE DISORDERS - Processes are provided that include additive compounds suitable for detecting the activity of an enzyme such as a lysosomal storage enzyme where the additive is a salt of oleic acid. Inclusion of a salt of oleic acid unexpectedly improves enzyme activity and reproducibility. | 03-03-2016 |
| 20160083775 | COMPOSITIONS AND METHODS FOR IDENTIFYING ENZYME AND TRANSPORT PROTEIN INHIBITORS - The invention is directed to compositions, e.g., cell-based and multiplexed platforms, to screen for small molecule drugs that inhibit enzymes such as proteases, e.g., viral proteases, e.g., HIV proteases; and methods for making and using these compositions. The invention provides compositions and methods for identifying compositions, e.g., drug molecules, that can inhibit proteases, e.g., viral proteases such as HIV proteases. In alternative embodiments, the invention provides cell-based platforms or assays to screen for compositions, e.g., small molecules or drugs, that inhibit or modify the activity of enzymes such as calcium-dependent protein convertases involved in HIV envelope protein processing, including cleavage of the HIV gp160 envelope precursor, resulting in gp120 and gp41 envelope products. In one embodiment, the invention provides a cell-based or multiplexed platform for monitoring the activity of enzymes, e.g., proteases such as viral proteases. | 03-24-2016 |
| 20160090576 | MATERIALS AND METHODS FOR CHARACTERIZING AND USING KASIII FOR PRODUCTION OF BI-FUNCTIONAL FATTY ACIDS | 03-31-2016 |
| 20160097075 | HIGH THROUGHPUT ASSAY FOR MONITORING AMP PRODUCTION AND AMINOACYL-tRNA SYNTHETASE ACTIVITY - A method of conducting an enzymatic reaction assay involving adenosine 5′-monophosphate (AMP) comprising the steps of reacting one or more compounds and producing AMP, deaminating the AMP to produce IMP, and oxidating the IMP by NAD+ to produce XMP and NADH. | 04-07-2016 |
| 20160145280 | TUNABLE FLUORESCENCE USING CLEAVABLE LINKERS - The invention relates to cleavable chemistry in general, and in particular, to tunable fluorescence using cleavable linkers present in fluorochrome-quencher conjugates. | 05-26-2016 |
| 20160194647 | Expression Vector | 07-07-2016 |
| 20160201045 | Endo-xylanase and Coding Gene and Use Thereof | 07-14-2016 |
| 20160252509 | CYSTEINE-REACTIVE LIGAND DISCOVERY IN PROTEOMES | 09-01-2016 |
