Class / Patent application number | Description | Number of patent applications / Date published |
435317100 | MISCELLANEOUS (E.G., SUBCELLULAR PARTS OF MICRO-ORGANISMS, ETC.) | 21 |
20080220517 | SITE-SPECIFIC CELL PERFORATION TECHNIQUE - A technique for controlling membrane denaturation reactions other than physical shear force was developed. For example, the present invention provides, a method for causing membrane disruption at a specific site by reacting a stimulus such as light with a compound that is activated by the stimulus, where the reaction occurs on a membrane such as a biomembrane. It also provides a membrane structure such as cells in which a specific site has been disrupted, which are obtained by the present method. Introduction of substances such as genes also became possible by using this membrane structure. Further provided is a membrane-destroying member for disrupting a membrane at a specific site. Thus, the present invention enabled, for example, easy membrane penetration using components constituting microelectrodes, micromanipulators, and microinjectors, which were conventionally hardly usable in penetrating cell membranes. | 09-11-2008 |
20090081775 | Microscope system and screening method for drugs, physical therapies and biohazards - Method and device for automated cell analysis and determination of transport and communication between living cells by analyzing the formation of tunneling nanotubes (TNTs) between cells. This method comprising the steps of singularizing cells in a culture medium and staining the cells with a fluorescent or luminescent dyes for staining of cytoplasm and membranes as well as TNTs, flagella and other cell particles for 3-D cell microscopy. The method comprises further an image analysis system. | 03-26-2009 |
20090123997 | MODULATORS OF ODORANT RECEPTORS - The present invention relates to polypeptides capable of promoting odorant receptor cell surface localization and odorant receptor functional expression. The present invention further provides assays for the detection of ligands specific for various odorant receptors. Additionally, the present invention provides methods of screening for odorant receptor accessory protein polymorphisms and mutations associated with disease states, as well as methods of screening for therapeutic agents, ligands, and modulators of such proteins. | 05-14-2009 |
20100248354 | MEMBRANE VESICLE FISSION SYSTEM - A membrane vesicle fission system is disclosed. The membrane vesicle fission system includes: a membrane vesicle having a lipid bi-layer membrane including a cationic lipid; and a unit which applies, to the membrane vesicle, an anionic material having a hydrophobic group. | 09-30-2010 |
20100304476 | Expression of Human Serum Albumin in Plastids - Human Serum Albumin (HSA) or an HSA fusion protein is expressed in plant plastids. A plastid transformation vector is made which contains an expression cassette that contains regulatory sequences, the coding region for HSA or an HSA fusion protein and a selectable marker coding sequence. The vector is used to transform a plant where the plant expresses the HSA or HSA fusion protein. HSA is isolated and purified from the plant. A preferred plant is tobacco. | 12-02-2010 |
20110086421 | USE OF BIOLOGICAL PHOTORECEPTORS AS DIRECTLY LIGHT-ACTIVATED ION CHANNELS - Use of a biological photoreceptor as light-controlled ion channel for the alteration of the ion conductivity of a membrane by means of light. The photoreceptor used comprises an apoprotein and a light-sensitive polyene covalently bound to the apoprotein, said polyene interacting with the apoprotein and functioning as a light-sensitive gate. | 04-14-2011 |
20110136220 | Method for Isolating Nuclei - This invention relates to methods of isolating cell nuclei from the other cell components in cell samples, e.g., cell samples from cell cultures or tissue samples. The method does not comprise ultracentrifugation or super-centrifugation rather the method comprises centrifuging cell samples in a table-top conventional centrifuge or microfuge. The method also comprises the use of buffers that are substantially devoid of protease inhibitor or enzyme treatments. The methods facilitate separation of nuclei from nuclear outer membranes leaving the cellular structures and inner membranes of nuclei intact. The method also provides for rapid and consistent results. | 06-09-2011 |
20110294203 | CELL WASHING - A cell cluster from which unwanted components are removed by efficiently washing the cell cluster is collected. Provided is a cell washing method including a centrifuging step (S | 12-01-2011 |
20120077263 | METHODS AND MATERIALS FOR ISOLATING EXOSOMES - This document relates to methods and materials involved in obtaining exosomes. For example, methods and materials for obtaining exosomes from biological samples such as urine samples are provided. | 03-29-2012 |
20120288930 | Lipid Pathway Modification in Oil-Bearing Microorganisms - The invention provides methods of modifying the lipids produced by microbial organisms through genetic engineering. The invention also provides genetically engineered microbes and methods of fermenting microbes for oil production. Also provided are oils, fuels, oleochemicals, chemical precursors, and other compounds manufactured by such modified microorganisms. Exemplary oil-bearing organisms include organisms containing one or more exogenous genes encoding a fatty acyl-ACP thioesterase, fatty acyl-CoA/aldehyde reductase, fatty acyl-CoA reductase, fatty aldehyde reductase, fatty aldehyde decarbonylase, and/or an acyl carrier protein. | 11-15-2012 |
20120315695 | Recovery of sugars from ionic liquid biomass liquor by solvent extraction - The present invention provides for a composition comprising a solution comprising (a) an ionic liquid (IL) or ionic liquid-aqueous (ILA) phase and (b) an organic phase, wherein the solution comprises a sugar and a boronic acid. The present invention also provides for a method of removing a sugar from a solution, comprising: (a) providing a solution comprising (i) an IL or ILA phase and (ii) an organic phase, wherein the solution comprises an IL, a sugar and a boronic acid; (b) contacting the sugar with the boronic acid to form a sugar-boronic acid complex, (c) separating the organic phase and the aqueous phase, wherein the organic phase contains the sugar-boronic acid complex, and optionally (d) separating the sugar from the organic phase. | 12-13-2012 |
20130052727 | COMPOSITIONS AND METHODS FOR AUTOLOGOUS GERMLINE MITOCHONDRIAL ENERGY TRANSFER - Oogonial stem cell (OSC)-derived compositions, such as nuclear free cytoplasm or isolated mitochondria, and uses of OSC-derived compositions in autologous fertility-enhancing procedures are described. | 02-28-2013 |
20130217114 | RAPID MEMBRANE ISOLATION METHOD FOR ANIMAL AND PLANT SAMPLES - Disclosed herein are methods for quickly obtaining crude membranes from cells, including animal and plant cells. The methods include incubating cells in a buffer and forcing the cells through a filter that causes rupture of the cells, and then separating the resulting crude membranes from most cytosolic proteins. | 08-22-2013 |
20140004601 | METHOD OF PURIFYING EXOSOMES | 01-02-2014 |
20140093954 | IMMUNOMODULATORY MINICELLS AND METHODS OF USE - The present disclosure is related to immunomodulatory bacterial minicells and methods of using the minicells. | 04-03-2014 |
20140356943 | Reprogrammation of Eukaryotic Cells with Engineered Microvesicles - The present invention relates to a non-genetic, detergent-free, bacteria-free method for reprogramming a eukaryotic cell, in particular for obtaining induced pluripotent stem cells (iPS), by using engineered microvesicles carrying at least one reprogramming transcription factor, wherein said engineered microvesicles are virus-free. | 12-04-2014 |
20150093820 | METHODS FOR DETECTION OF TARGET USING AFFINITY BINDING - A method of recovering a target from a sample is provided. The method comprises the adding a substrate coupled binding element to the sample comprising the target to form a substrate coupled binding element-target complex; precipitating the complex by changing one or more environmental conditions of the substrate and recovering the target and the substrate coupled binding-element under mild conditions. | 04-02-2015 |
20160160237 | Mitochondrial Collection and Concentration, and Uses Thereof - Preparations and methods of producing a concentrated mitochondrial preparation using inverted phase centrifugation are disclosed. The methods include centrifuging a closed-bottom tube containing a heavy phase including a mitochondrial suspension in the portion of the tube proximal to the axis of rotation and a light phase in the portion of the tube distal to the axis of rotation. The centrifugation causes the suspension to form a concentrated mitochondrial layer at the interface between the heavy layer and the light layer, and the fluid at this interface is collected to produce the concentrated mitochondrial preparation. The concentrated mitochondrial preparation can be used for various applications, including in vitro fertilization applications. | 06-09-2016 |
20160200786 | BH3 PEPTIDES AND METHODS OF USE THEREOF | 07-14-2016 |
20170233766 | METHOD FOR ENHANCING EXTRACELLULAR VESICLE PRODUCTION | 08-17-2017 |
20220133803 | METHOD AND SYSTEM FOR ISOLATION OF MESENCHYMAL STEM CELL EXOSOMES - Disclosed is a system and method for isolating large quantities of viable, undamaged exosomes from liquid, cell-free mesenchymal stem cell cultures using tangential flow filtration. | 05-05-2022 |