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Culture, maintenance, or preservation techniques, per se

Subclass of:

435 - Chemistry: molecular biology and microbiology

435410000 - PLANT CELL OR CELL LINE, PER SE (E.G., TRANSGENIC, MUTANT, ETC.); COMPOSITION THEREOF; PROCESS OF PROPAGATING, MAINTAINING, OR PRESERVING PLANT CELL OR CELL LINE; PROCESS OF ISOLATING OR SEPARATING A PLANT CELL OR CELL LINE; PROCESS OF REGENERATING PLANT CELLS INTO TISSUE, PLANT PART, OR PLANT, PER SE, WHERE NO GENOTYPIC CHANGE OCCURS; MEDIUM THEREFORE

Patent class list (only not empty are listed)

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Class / Patent application numberDescriptionNumber of patent applications / Date published
435420000 Culture, maintenance, or preservation techniques, per se 54
20080261309IN VITRO MULTIPLICATION OF HOODIA PLANTS - A tissue culture micropropagation process for 10-23-2008
20080261310IN VITRO ROOTING OF HOODIA PLANTS - An in vitro tissue culture process of rooting 10-23-2008
20090023214Rescue of Plant Cell Cultures and Suspensions After Cryopreservation-Induced Damage - The subject invention relates to recovery of cryopreserved plant cell cultures after cryopreservation. The use of canine IL-4 and human gamma interferon is exemplified in some preferred embodiments.01-22-2009
20090104701METHOD FOR THE PRODUCTION OF BIOMASS FROM PLANT DIFFERENTIATED TISSUE - The present invention provides a method and a culture medium for the production of food biomass by directly culturing seed kernel tissue, or seed cotyledonary differentiated tissue. The culture medium of the present invention contains at least DKW culture medium, Vitamin MS culture medium with an enriched concentration of thiamine, sacarose, kinetine, adenine, 2,404-23-2009
20100233813PLANT STEM CELL LINE DERIVED FROM CAMBIUM OF HERBACEIOUS PLANT WITH STORAGE ROOT AND METHOD FOR ISOLATING THE SAME - The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time. Accordingly, the cell line derived from the cambium of an herbaceous plant having a storage root makes it possible to produce large amounts of useful plants which are difficult to cultivate outdoor due to various problems associated with the period of cultivation, the selection of cultivation land, cultivation cost and the like.09-16-2010
20100248370METHOD AND APPARATUS FOR TISSUE TRANSFER - A handheld tool is disclosed which may be used to transfer a plurality of plant tissue explants from a first container to a second container. The handheld tool may include a disposable tip member which couples the plurality of plant tissue explants through use of negative pressure. An automated system which transfers a plurality of plant tissue explants from a first container to a second container is also disclosed. The automated system may include a first presentment system which moves the first container to a region, a second presentment system which moves the second container to the region, and a robot system that transfers the plurality of plant tissue explants from the first container to the second container.09-30-2010
20100267144SYSTEMS AND METHODS FOR CONTROLLED PERVAPORATION IN HORTICULTURAL CELLULAR TISSUE - A hypobaric apparatus and methods capable of inducing and maintaining in stored horticultural cellular tissue controlled pervaporation of undesired chemical compounds without reaching the boiling point of intracellular water molecules, comprising a hypobaric chamber constructed to provide a leak rate of less than 4.0 mm Hg per hour, sensors and regulators coupled to the chamber and to a controller to measure and regulate target correlates. Commodities processed, including processing storage periods longer than any known involving controlled atmosphere, exhibit an absence of low oxygen injury, high carbon dioxide injury, chilling injury, leaf abscission, leaf de-greening, fungal decay, bacterial decay, gravitational curving, geotropic curvatures, leaf epinasty, stem epinasty, flower fading, senescence, live invertebrates at any life stage, fumigant chemical compounds, volatile fungicides, volatile bactericides; no advance in ripening; minimal water loss.10-21-2010
20110212525METHOD AND APPARATUS FOR SUBSTANTIALLY ISOLATING PLANT TISSUES - The present invention provides methods and devices for the rapid isolation of monocot plant embryos suitable for transformation or tissue culture. The invention includes mechanical devices for substantially isolating plant embryos for use as transformable explants. Media suitable for isolating plant embryos and methods for their preparation are also provided.09-01-2011
20120122224DEVICE FOR PERFORMING PHOTOCHEMICAL PROCESSES - Device for carrying out photochemical processes on a microscale and use of the device for photochemical reactions and culturing photosynthesizing cells and/or microorganisms.05-17-2012
20120220036Media, Kits, Systems and Methods for the Micropropagation of Bamboo - Disclosed herein are media, kits, systems and methods for achieving micropropagation of bamboo on a commercially-relevant scale.08-30-2012
20120276633SUPPLYING TREATED EXHAUST GASES FOR EFFECTING GROWTH OF PHOTOTROPHIC BIOMASS - There is provided a process for growing a phototrophic biomass in a reaction zone. The process includes treating an operative carbon dioxide supply-comprising gaseous material feed so as to effect production of a carbon dioxide-rich product material. The carbon dioxide concentration of the carbon dioxide-rich product material is greater than the carbon dioxide concentration of the operative carbon dioxide supply-comprising gaseous material feed. Production of at least a fraction of the operative carbon dioxide supply-comprising gaseous material feed is effected by a gaseous exhaust material producing process. At least a fraction of the carbon dioxide-rich product material is supplied to the reaction zone so as to effect growth of the phototrophic biomass by photosynthesis in the reaction zone.11-01-2012
20120309090SELF CONTAINED SOLID PHASE PHOTOBIOREACTOR - A compressible bioreactor for cultivating photosynthetic microorganisms, the bioreactor comprising a feeder trough, a collection trough, a growth fabric, and a barrier layer, where the bioreactor has a compressed mode and an extended mode, the growth fabric is coupled to the feeder trough and the collection trough, the growth fabric is substantially extended in the extended mode of the bioreactor, the growth fabric is substantially compressed in the compressed mode of the bioreactor and the barrier layer is coupled to the feeder base and the collection base that encases the growth fabric in a substantially airtight environment12-06-2012
20130102076Systems and methods for growing photosynthetic organisms - Methods and apparatus for promoting the growth of an aquatic photosynthetic organism within a growth medium in a photobioreactor may use a luminescent material targeting the aquatic photosynthetic organism in the photobioreactor. The luminescent material may be a substrate with a matrix of conductors coupled to the substrate, and light emitting diodes (“LEDs”) electrically coupled to the matrix of conductors. The aquatic photosynthetic organism in the photobioreactor is exposed to the light emitted by the LEDs.04-25-2013
20130137178Plant Stem Cell Line Derived from Cambium of Herbaceous Plant with Storage Root and Method for Isolating the Same - The present invention relates to a cell line derived from the cambium of an herbaceous plant having a storage root and a method for isolating the same. More specifically, relates to a cambium-derived homogeneous cell line having the ability to divide, which is obtained from the cambium-containing storage tissue of an herbaceous plant having a storage root without a separate dedifferentiation process, and to a method for isolating the same. The cell line derived from the cambium of an herbaceous plant having a storage root has an active division ability and is homogeneous. Also, it is stable during culture, because it has not undergone a dedifferentiation process. Thus, through the optimization of proliferation thereof, the cell line can be allowed to proliferate in a large amount within a short time.05-30-2013
20130323843POLYPHENOL PRODUCTION BY VACCINIUM MYRTILLUS CELL CULTURES - Cell cultures of 12-05-2013
20140178998Photobioreactor and a process for enhancing growth of photosynthetic organisms - A photobioreactor apparatus in which a reaction chamber contains a slurry of water, nutrients and photosynthetic organisms. The sidewall of the reaction chamber is constructed of translucent material, thereby permitting light to be conveyed into the slurry to promote photosynthesis. The interior of the reaction chamber is in fluid communication with a gas dispenser. A lift member is disposed in the reaction chamber and gas is introduced in the slurry beneath the lift member, thereby providing buoyancy to lift the lift member, thereby providing agitation and movement of the slurry within the photobioreactor and enhancing growth of photosynthetic organisms.06-26-2014
20140329324FUNCTIONAL UNLIMITED CULTURE MEDIUM HAVING A NATURAL BIO-COMPOSITE INDUCER - A culture medium with natural inducer for biocomposites. In a general aspect the present disclosure is an indefinite functional culture medium with a natural inducer for biocomposites comprising: i) biomass plant incorporating high copper content, vitamins and trace minerals; and ii) a natural inducer, that is vegetable vinegar.11-06-2014
20150093829CULTURE HARVESTING IN AUTOMATED BIOREACTOR SYSTEM - A system for harvesting totipotent plant tissue culture can include a sterile enclosure, a plurality of bioreactors in the sterile enclosure, at least one agitator in the sterile enclosure, and a culture harvest system. The at least one agitator can be configured to agitate culture within the plurality of bioreactors. The culture harvest system can include tubing configured to connect to a port of one of the plurality of bioreactors in the sterile enclosure, a culture harvest container, and a pump configured to pump culture from the one of the plurality of bioreactors into the culture harvest container via the tubing.04-02-2015
20160002686PLANT CELL CULTURE FOR PRODUCTION OF NATURAL PRODUCTS WITH REDUCED FLUCOSINOLATE CONTAMINATION - Disclosed are methods for obtaining a natural product preparation with reduced glucosinolate contamination from a plant of Brassicaceae. The methods can include cultivating a plant callus from a plant capable of producing a desired natural product, selecting a callus with reduced glucosinolate production, and cultivating the selected callus in a liquid medium. The method can also include recovering the natural product from the culture. Also disclosed are methods for obtaining cabbage anthocyanin with reduced glucosinolate contamination. The methods can include cultivating a red cabbage plant callus with reduced glucosinolate production in a liquid medium to obtain a suspension culture and cultivating the suspension culture in a medium lacking a nitrogen source. The method can also include recovering the anthocyanin with reduced glucosinolate contamination from the culture. Finally, several specific low-glucosinolate cell lines are described.01-07-2016
20160106091FREEZE PRESERVATION OF LIVING CELLS - A method is for the cryopreservation of a (plant) cell, (plant) organelle or (plant) tissue, where the (plant) cell, (plant) organelle or (plant) tissue is encapsulated in a matrix formed of an abiotic material in presence of one or more additives with cryoprotectant effect. The method provides a faster, more effective and cheaper way to cryopreserve biological material.04-21-2016
435421000 Involving protoplast 1
20110059529METHODS FOR GENETIC PLANT TRANSFORMATION USING WATER-SOLUBLE FULLERENE DERIVATIVES - In various embodiments, methods described herein comprise the use of water-soluble cationic fullerene derivatives for improving plant genetic transformation. Cationic Fullerene derivatives of the invention possess DNA binding and compaction activity and provide a new method to deliver DNA into plant cells for plant transformation. Water-soluble fullerene derivatives of the invention with anionic or non-polar substituents possess antioxidant (free radical scavenging) activity, provide improved yields and efficiency of plant transformation methods such as biolistic, 03-10-2011
435422000 Involving conifer cell or tissue (e.g., pine, spruce, fir, cedar, etc.) 15
20080206868Methods for storing conifer somatic embryo germinants - In one aspect, the present invention provides methods of storing conifer somatic embryo germinants germinated on a sterile germination medium for delayed transplanting into a growth medium. The methods of the invention comprise the steps of: (a) placing the germinants while still on sterile germination medium into a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months, said germinants comprising a visible, well-defined epicotyl and radicle; and (b) placing the germinants in water for a time greater than about 1 hour at a temperature below about 24° C. prior to transplantation into growth medium.08-28-2008
20080213896Methods for storing conifer somatic embryo germinants - In one aspect, the present invention provides methods of storing conifer somatic embryo germinants germinated oil a sterile germination medium for delayed transplanting into a growth medium. The methods of the invention comprise the steps of: (a) placing the germinants while still on sterile germination medium into a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months, said germinants comprising a visible, well-defined epicotyl and radical; and (b) placing the germinants in water for a time greater than about 1 hour at a temperature below about 24° C. prior to transplantation into growth medium.09-04-2008
20080213897Methods for storing conifer somatic embryo germinants - In one aspect, the present invention provides methods of storing conifer somatic embryo germinants germinated on a sterile germination medium for delayed transplanting into a growth medium. The methods of the invention comprise the steps of: (a) placing the germinants while still on sterile germination medium into a cold environment in which the temperature is in the range of 0.5° C. to 10° C. for a time period up to six months, said germinants comprising a visible, well-defined epicotyl and radicle; and (b) placing the germinants in water for a time greater than about 1 hour at a temperature below about 24° C. prior to transplantation into growth medium.09-04-2008
20090087908Methods for Stratification and Storage of Somatic Embryos - In one aspect, a method is provided for producing stratified cotyledonary conifer somatic embryos. The method comprises (a) incubating a culture comprising immature conifer somatic embryos in a culture vessel comprising a development medium having an osmolality in the range of from 300 mM/Kg to 450 mM/Kg at a temperature of from 22° C. to 25° C. for a first incubation period sufficient in length for at least a portion of the embryos to reach anatomical maturity; and (b) subjecting the embryos in the culture vessel in accordance with step (a) to a temperature of from 0° C. to 10° C. for a second incubation period of at least one week lo produce stratified cotyledonary somatic embryos.04-02-2009
20090087909Use of Trehalose in Conifer Somatic Embryogenesis to Increase Germination Vigor - In one aspect, a method is provided for increasing germination vigor of conifer somatic embryos produced in vitro. The method comprises (a) culturing a plurality of immature conifer somatic embryos for a first incubation period in, or on, a first development medium that comprises less than 0.1% trehalose; and (b) culturing the plurality of immature conifer somatic embryos treated in accordance with step (a) for a second incubation period in, or on, a second development medium that comprises at least 0.1% trehalose.04-02-2009
20090280566METHODS FOR INCREASING GERMINATION FREQUENCY AND/OR VIGOR BY COLD SHOCK TREATMENT OF CONIFER SOMATIC EMBRYOS DURING DEVELOPMENT - In one aspect, a method is provided for increasing germination vigor and/or frequency of conifer somatic embryos produced in vitro. The method comprises (a) incubating a plurality of immature conifer somatic embryos for a first incubation period in, or on, a first development media at a temperature in the range of 20° C. to 30° C.; (b) exposing the plurality of immature conifer somatic embryos incubated in accordance with step (a) to a cold temperature in the range of 0° C. to 10° C. for a time period of at least one week; and (c) incubating the plurality of immature conifer somatic embryos treated in accordance with step (b) for a second incubation period in, or on, a second development medium at a temperature in the range of 20° C. to 30° C.11-12-2009
20110229967METHOD OF CULTURING CONIFER SOMATIC EMBRYOS USING S(+)-ABSCISIC ACID - A method for promoting maturation and development of vigorous conifer (gymnosperm) somatic embryos comprising the use of S(+)-ABA as the substantive form of ABA.09-22-2011
20120276634 SOMATIC EMBRYOGENESIS AND EMBRYO HARVESTING AND METHOD AND APPARATUS FOR PREPARING PLANT EMBRYOS FOR PLANT PRODUCTION - Described herein are methods and media for facilitating somatic embryogenesis and for collecting, conditioning, and transferring the washed embryos onto a substrate and into an environment suitable for conditioning the embryos for a desired period of time so they become germination-competent for plant production. The described plant embryo cleaning apparatus and method are used for preparing multiple plant embryos for plant production. The apparatus and method can use a cleaning fluid source, a fluid-conditioning system, a fluid-delivery structure, a cleaning station, an outlet mechanism, a negative pressure source, and a controller.11-01-2012
20130065306METHOD FOR MATURING AND SYNCHRONIZING CONIFER SOMATIC EMBRYOS - A method for the culture of somatic plant embryos comprising contacting said embryos with a culture medium containing a plasmolytic or near plasmolytic concentration of a sugar alcohol or a combination of sugar alcohols; and culturing said embryos in said culture medium for a time period suitable for the desired level of maturation to be achieved. Inositol is a preferable sugar alcohol.03-14-2013
20130078722METHODS OF MULTIPLYING CONIFER EMBRYOGENIC TISSUE - The invention provides methods for multiplying conifer embryogenic tissue. The methods of the invention comprise the steps of: (a) culturing conifer embryogenic tissue in or on a solid multiplication medium comprising one or more growth hormones; and (b) culturing the conifer embryogenic tissue multiplied in step (a) in or on a liquid multiplication medium comprising one or more growth hormones, wherein the initial concentration of growth hormones in the solid multiplication medium of step (a) is greater than the initial concentration of growth hormones in the liquid multiplication medium of step (b).03-28-2013
20140004606METHODS OF TRANSFERRING PLANT EMBRYOS TO GERMINATION MEDIUM01-02-2014
20140178999METHODS OF INITIATING PLANT SOMATIC EMBRYOS - Methods of initiating plant somatic embryos from megagametophytes are provided.06-26-2014
20140186956METHODS OF SORTING PLANT EMBRYOS - Methods of sorting plant somatic embryos according to germination potential are provided.07-03-2014
20150305248METHODS OF TRANSFERRING PLANT EMBRYOS TO GERMINATION MEDIUM - Methods of transferring a plurality of plant somatic embryos to germination medium are provided.10-29-2015
20160198657LATE EMBRYO DEVELOPMENT AND MATURATION AT COLDER TEMPERATURE07-14-2016
435425000 Involving tobacco cell or tissue 1
20100311168NANOPARTICLE MEDIATED DELIVERY OF SEQUENCE SPECIFIC NUCLEASES - Provided are methods for introducing a sequence specific nuclease into a plant cell comprising a cell wall. Methods are provided for genetically or otherwise modifying plants and for treating or preventing disease in plant cells comprising a cell wall.12-09-2010
435430000 Involving regeneration or propagation into a plant or plant part 17
20080280361PREPARATION AND USE OF PLANT EMBRYO EXPLANTS FOR TRANSFORMATION - The present invention relates to excision of explant material comprising meristematic tissue from seeds, and storage of such material prior to subsequent use in plant tissue culture and genetic transformation. Methods for tissue preparation, storage, and transformation are disclosed, as is transformable meristem tissue produced by such methods, and apparati for tissue preparation.11-13-2008
20100068812METHOD FOR INCREASING TRANSFORMATION EFFICIENCY IN PLANTS, COMPRISING COCULTURE STEP FOR CULTURING PLANT TISSUE WITH COCULTURE MEDIUM CONTAINING 3, 6-DICHLORO-O-ANISIC ACID - The present invention aims to provide a method for increasing transformation efficiency in plants when compared to conventionally known 03-18-2010
20100273264APPARATUS FOR AND METHOD OF PREPARING PLANT TISSUE FOR PLANT PRODUCTION - An apparatus for preparing plant tissue (e.g., somatic embryos, embryogenic tissue, organogenic tissue, vegetative tissue, seeds, etc.) for plant production includes a first station having a first rack system configured to support at least one culture vessel, a second station having an automated member configured to manipulate the at least one culture vessel and a third station having a second rack system configured to support the at least one culture vessel after being manipulated by the automated member. The second station can be selectively adjusted to perform more than one operation required in the plant development. According to exemplary embodiments, the apparatus may include more than one second station (e.g., operational stations, etc.).10-28-2010
20110124107METHOD OF HIGH FREQUENCY REGENERATION OF SORGHUM - The present invention relates generally to the regeneration of 05-26-2011
20120156784Methods for Improving Competency of Plant Cells - The present invention provides methods for improving competency of plant cells for bacterial-mediated transformation comprising contacting the plant cells with an effective amount of polyethylene glycol (PEG) for a period of time prior to transformation. The ability to store and maintain competent plant cells for transformation and tissue culture allows more efficient planning and execution of large-scale experiments by providing flexibility of peak production hours, or during unplanned disruptions in the production process. These methods are useful in preserving the viability of plant cells in various storage conditions, thus improving their competency for transformation and tissue culture.06-21-2012
20120264216MEDIA, KITS, SYSTEMS AND METHODS FOR THE MICROPROPAGATION OF BAMBOO - Disclosed herein are media, kits, systems and methods for achieving micropropagation of bamboo on a commercially-relevant scale.10-18-2012
20120329158Methods of Plant Regeneration and Apparatus Therefor - A method of preparation of a plant tissue fragment is provided wherein apical dominance of plant meristematlc tissue is inhibited followed by fragmentation of the tissue. Also provided are methods of plant micropropagation and methods of artificial seed production using apical dominance suppression in preferably, a semi-automated process. Also provided is a plant tissue processing machine that generates plant fragments with high regeneration efficiency and an artificial seed production apparatus.12-27-2012
20130071933CITRUS SHOOT REGENERATION COMPOSITIONS, METHODS, AND SYSTEMS - The present disclosure relates, according to some embodiments, to citrus shoot regeneration compositions, methods, and systems. For example, citrus shoot regeneration compositions (e.g., culture media) may comprise one or more non-ionic surfactants. Methods may comprise preparing and using these compositions in some embodiments. Regeneration systems may comprise, in some embodiments, one or more of these compositions and/or one or more citrus explants.03-21-2013
20140179000PLANT CULTIVATION METHOD - Method for the production of young plants and/or of micro-parent stock of herbaceous ornamentals, which comprises an in vitro culture phase during which explants obtained from parent stock of species to be propagated, or derivatives of these explants, are subjected to micropropagation which is carried out under suitable conditions and on suitable culture media, in order to produce micro-plantlets which, when subjected to an in vivo culture phase, are intended to develop into plants or into micro-parent stock, characterized in that, to carry out said micropropagation: 06-26-2014
20140212975COMPOSITIONS AND METHODS FOR BIOCULTURE OF WASABIA JAPONICA - The present invention provides media, kits, systems, and methods for achieving large scale wasabi production within a short time via bioculture. The present invention for wasabi production results in shorter tuber development phase and higher yield.07-31-2014
435430100 Involving callus or embryonic stage 7
20090111182Method for Propagation of Plant - A novel method is provided, which makes it possible to perform mass propagation of a plant of the family Araceae, the family Bromeliaceae, or the family Marantaceae. Specifically, a method for producing an embryogenic callus, comprising culturing a leaf sheath or a part thereof of a plant selected from the group consisting of the family Araceae, the family Bromeliaceae, and the family Marantaceae as an explant and then inducing an embryogenic callus and a propagation method comprising regenerating a plant through induction of a somatic embryo from the embryogenic callus obtained by the above method are also provided herein.04-30-2009
20100297770LIQUID-BASED METHOD FOR PRODUCING PLANT EMBRYOS - The present invention relates to methods for developing embryos and producing germination-competent embryos using a liquid embryo development media.11-25-2010
20110076772METHODS OF PREPARING NUTRITIVE MEDIA FOR GROWTH AND/OR GERMINATION OF PLANT EMBRYOS - In one aspect, a method is provided for producing an improved nutritive medium comprising an adsorbent material for culturing plant embryos.03-31-2011
20140134735Plant Cell Differentiation Promoter - The present invention addresses the problem of providing a plant cell differentiation promoter with which it is possible to promote differentiation from a callus to a normal adventitious embryo, or promote differentiation of an adventitious root or adventitious bud from a plant cutting, and as a result, obtain a regenerated plant with stability. The present invention provides a plant differentiation promoter comprising as the active ingredient a specific ketole fatty acid or derivative thereof.05-15-2014
20140287508METHOD FOR APPLICATIONS OF NANO-SIZED MATERIALS AS CELL GROWTH REGULATORS / CELL GROWTH ENHANCERS FOR PLANT, AND AGENT INCLUDING NANO-SIZED MATERIALS - One aspect of the disclosure is directed to a method for activation/enhancement of cell growth of a plant. The method includes providing a nano-sized material contained agent, and treating the plant with the nano-sized material contained agent to allow sufficient interaction of cells of the plant with the nano-sized material so as to activate/enhance the cell growth of the plant.09-25-2014
20150132852TISSUE CULTURING METHOD, CULTURING METHOD OF FERNS AND EXPLANT OBTAINED THEREFROM - A tissue culturing method includes following steps: providing a chopped gametophyte, generating calluses by culturing the chopped gametophyte, and performing apogamic regeneration of sporophytes, by culturing the calluses in a culture fluid to develop the sporophytes from the calluses. The present invention also provides a tissue culturing method of ferns and an explant.05-14-2015
20150291517BENZENESULFONAMIDE COMPOUNDS FOR SOMATIC EMBRYOGENESIS IN PLANTS - Benzenesulfonamide compounds potentiate 2,4-D induced embryogenesis in plants. In particular, 4-chloro-N-methyl-N-(2-methylphenyl)benzenesulfonamide and analogs induce somatic embryogenesis in plants. Methods of inducing somatic embryogenesis comprise exposing selected plant tissues, e.g. seed embryos, to auxins, e.g. 2.4-D and the benzenesulfonamide compounds. Compounds can be prepared by reacting sulfonyl chloride, an amine and pyridine in CH10-15-2015
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