| Entries |
| Document | Title | Date |
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| 20080199956 | Storage Medium For Cells - The invention relates to a method for storages of human cells in particular, cell media for storage of cells and the use of such cell media for storage of cells, wherein the vitality of the cells is preserved during the storage. | 08-21-2008 |
| 20080220520 | CRYOPRESERVATION OF HUMAN EMBRYONIC STEM CELLS IN MICROWELLS - The present invention relates to methods and structures for preparing stem cells for use in cryopreservation methods. Stem cell colonies are provided between first and second matrix portions and are exposed to a carbohydrate-containing cryoprotecting medium and a freezing medium. The methods of the invention yield cryopreserved cells that maintain cell viability and exhibit limited cell differentiation after freezing and thawing, facilitating storage, shipping and handling of embryonic stem cell stocks and lines for research and therapeutics. | 09-11-2008 |
| 20080286865 | Methods for the Preservation of Platelet Efficacy During Storage - Invented is a method for the preservation of human platelet lifespan and/or efficacy during storage which comprises the addition of an effective amount of a non-peptide TPO receptor agonists to a storage solution containing human platelets. | 11-20-2008 |
| 20090011505 | METHOD AND DEVICE FOR THE CRYO-CONSERVATION OF SAMPLES - A method and a device for the cryo-conservation of a hydrous sample, in particular biological sample, wherein the sample is introduced into a cavity of a container, remaining free space of the cavity if any is filled with a hydrous liquid, the cavity is closed in a liquid-tight manner and the container is held in a holding device, and the container together with the sample is cooled to a temperature below 251 K, preferably 173 K, for example by means of liquid nitrogen, propane or ethane, wherein the container and the holding device prevent an expansion of the cavity in volume. The selected cooling rate is sufficiently high so that a crystal formation of the freezing water is suppressed in favor of glass formation. | 01-08-2009 |
| 20090023209 | SOLUTION FOR THE INDEFINITE MAINTENANCE OF NUCLEIC ACIDS IN THE CELL OF ORIGIN THEREOF - The invention relates to a product having characteristics which enable a biological sample obtained from an individual to be maintained under conditions such that the quality of nucleic acids later extracted is not affected, thereby guaranteeing the integrity of the sample regardless of the time that elapses between the sample being obtained and the nucleic acid being extracted. A solid (organic) or liquid (blood, semen, cell culture) biological sample from a eukaryote or prokaryote is mixed in a solution comprised of a mixture of trisodium ethylenediaminetetraacetic acid (EDTA), sodium fluoride (NaF), and saturation with thymol (extracted from | 01-22-2009 |
| 20090042288 | Method of Producing Organotypic Cell Cultures - The present invention relates to cell and tissue culture. In particular, the present invention provides a method for preparing an organotypic culture using dissociated cells or microexplants obtained from an animal organ. The method for preparing an organotypic culture comprises culturing cells from an organ on a surface characterised in that the cells are compacted. The invention further relates to a high-throughput method for the preparation of a collection of organotypic cultures. The invention further relates to a device for carrying out a method of organotypic culture according to the invention. | 02-12-2009 |
| 20090042289 | Preservation of Cells - The present invention relates to methods for preserving cells, in particular primary cells such as hepatocytes. In particular, the present invention relates to preserving cells within a gel comprising a hydrolysed gelatin and culturing the cells at a temperature between 0 and 15° C. | 02-12-2009 |
| 20090053806 | COATING SOLUTION AND METHOD FOR CAPTURING AND PRESERVING BIOLOGICAL MATERIALS - The present invention is directed to a coating solution for capturing and preserving biological materials. The coating solution includes at least one saccharide. The coating solution may also include at least one other constituent, such as amino acids, complex proteins, surfactants, and mixtures thereof. The present invention is also directed to providing a method for collecting and preserving biological material using a coating solution. | 02-26-2009 |
| 20090075375 | CHOROID PLEXUS PREPARATION AND USES THEREOF - The present invention is directed to the use of choroids plexus cells and/or choroids plexus conditioned media for enhancing the growth, survival and/or maintenance of function of non-choroid plexus cells grown in long term or short term culture. | 03-19-2009 |
| 20090081785 | Desiccated Biologics And Methods Of Preparing The Same - The present invention provides compositions comprising desiccated biologics comprising a cell, protein, virus, nucleic acid, carbohydrate, or lipid, or any combination thereof, along with at least one membrane penetrable sugar, and at least one membrane impenetrable sugar, wherein the moisture content is from 5% to 95%, and to methods of preparing the same, and to methods of treating animals using the same. | 03-26-2009 |
| 20090111178 | GENE REPORTER ASSAY, KIT, AND CELLS FOR DETERMINING THE PRESENCE AND/OR THE LEVEL OF A MOLECULE THAT ACTIVATES SIGNAL TRANSDUCTION ACTIVITY OF A CELL SURFACE PROTEIN - The present invention relates to a commercializable cell and to a gene reporter assay method and a kit which use this cell to determine the presence and/or the level of a molecule that activates signal transduction activity of a cell surface protein. This cell is treated in such a manner that it will have a sufficiently long shelf life for its intended purpose, whereupon at the end of its useful shelf life or at the end of its use, i.e., in an assay, the cell undergoes cellular death. | 04-30-2009 |
| 20090130755 | Hydrogel networks having living cells encapsulated therein - The present invention is directed to a hydrogel network comprised of a physically cross-linked polymer and a chemically cross-linked polymer or physically entangled copolymer containing living cells, such as chondrocytes, encapsulated therein. In a preferred aspect, the physically cross-linked polymer is selected from the group consisting of thermally gelling polysaccharides and proteins, such as agarose or gelatin, and the chemically cross-linked or physically entangled polymer is synthesized from a water-soluble vinyl monomer, either as a homopolymer or copolymer, such as polyethylene glycol diacrylate (“PEG-DA”) and 2-hydroxyethyl methacrylate (“HEMA”). | 05-21-2009 |
| 20090130756 | CRYOPRESERVATION OF CELLS USING CROSS-LINKED BIOACTIVE HYDROGEL MATRIX PARTICLES - The present invention is directed to methods of cryopreserving cells and cryopreserved cells prepared according to the methods. In specific embodiments, the method comprises combining cells with a cross-linked hydrogel matrix in particulate form, the matrix comprising a polyglycan cross-linked to a polypeptide and subjecting the combination to cryopreservation conditions. In further embodiments, the invention provides cell-seeded compositions comprising cells and a cross-linked bioactive hydrogel matrix in particulate form, the matrix comprising a polyglycan cross-linked to a polypeptide, wherein the composition has been subjected to cryopreservation conditions. The cryopreserved cells can be thawed and used in methods of treatment without the need for intervening steps to make the cells viable for in vivo use. | 05-21-2009 |
| 20090197331 | Compositions for cryopreservation of cells - A composition for the cryopreservation of cells according to the present invention at least comprises sericin and one or more components selected from the group consisting of amino acids and saccharides. Further, a method for the cryopreservation of cells according to the present invention comprises the steps of placing target cells in the abovementioned composition for the cryopreservation of cells and cryopreserving them. According to the composition for the cryopreservation of cells of the present invention, cells can be cryopreserved over a long period of time without the use of serum and components derived from serum. | 08-06-2009 |
| 20090239299 | AUTOLOGOUS TISSUE HARVESTING AND IRRIGATION DEVICE - The present invention includes a harvesting and irrigation device, a method and a kit for the collection of viable fat cells and/or adipose tissue with decreased handing and improved yield and viability. | 09-24-2009 |
| 20090286315 | PROCESS FOR PRESERVING INSULIN-SECRETING CELLS INTENDED TO BE TRANSPLANTED IN A PATIENT - Process for preserving insulin secreting cells intended to be transplanted, including the following steps: introducing an initial volume of culture medium and insulin-secreting cells into a culture container, providing a culture medium source, and replacing, at time intervals below 8 hours, the culture medium in the culture container with culture medium from the source so as to renew the culture medium contained in the container. | 11-19-2009 |
| 20090298172 | Histological specimen treatment apparatus and method - Method and apparatus for processing tissue specimens against decomposition, putrefaction and autolysis which use a simple three step procedure in a single vessel or container. First, the specimens are saturated with a solvent mixture of a ketone and a hydrocarbon, e.g., an Acetone/Hexane or an Acetone/Xylene mixture, to dissolve lipids and other cellular solutes. The container is then flooded with melted Paraffin. In a last step, the solvent mixture is vaporized and evacuated from the container, allowing the melted Paraffin to replace the vaporized solvent and impregnate the specimens. Raw, i.e., non-processed and non-burred specimens up to 5 mm thick can be processed in about 60 minutes. A solvent regenerator distills the evacuated solvent; and converts vent waste gases into carbon dioxide and water through a thermocatalytic oxidizer. | 12-03-2009 |
| 20100062528 | METHOD FOR PACKAGING CELLS IN CULTURE IN A LOW-PRESSURE ENVIRONMENT, AND CORRESPONDING DEVICE - Method for packaging cells in culture in a low-pressure environment, and corresponding device. The invention relates to a method for packaging live cells in culture for the purpose of transporting them, wherein said cells are in a culture medium contained in at least one container, characterized in that said method comprises: a phase of filling said container(s) according to a given degree of filling; a step of closure of said container(s) comprising: bagging said container(s) in a flexible sachet; and reducing the pressure inside said sachet; sealing said sachet, with the pressure reduction being maintained, wherein said closure step is carried out under temperature conditions correlated with said degree of filling with a view to avoiding the boiling of said culture medium during said pressure reduction. | 03-11-2010 |
| 20100099187 | METHOD FOR PRODUCING A VACCINE FOR THE TREATMENT OF CANCER - The present invention discloses a method for producing a haptenized vaccine from a tissue biopsy. The method includes obtaining a tissue biopsy, isolating the cells, irradiating the cells, haptenizing the cells, and cryopreserving the cells. The present invention also discloses a method for treating cancer using the vaccines produced by the methods described herein. | 04-22-2010 |
| 20100112690 | INSERT DEVICE FOR MULTIWELL PLATE - An assembly comprises a multiwell test plate having a plurality of wells having an opening and a base surface, an insert plate having a plurality of inserts positioned to align with a corresponding one of the wells whereby the insert plate can be nested with the multiwell plate. Each of the inserts includes a supply port arranged for flow communication with a supply source, an exhaust port, a bottom portion having a plurality of channels extending between the supply port and the exhaust port, and a gas permeable membrane covering the bottom portion. Each of the inserts is sized to position the gas permeable membrane a desired distance from the base surface of the multiwell test plate when the multiwell test plate and the insert plate are coupled to one another. | 05-06-2010 |
| 20100136686 | APPARATUS AND METHOD FOR CULTURING AND PRESERVING TISSUE CONSTRUCTS - A disclosure is made of various apparatus and methods for culturing and preserving cells and tissue in ways that minimize contamination potential, direct cells to reside in desired areas, allow uniform cell distribution during seeding, provide optimal growth conditions by controlling the amount of medium residing in proximity of cells, allow desired compounds and molecules to reside in proximity of the cells, allow co-culture, provide for efficient scale up, allow a desired shape of tissue to be created while retaining a closed system, and allow cryopreservation and reconstitution of cell and tissue while retaining a closed system. The apparatus and methods can be combined to prevent the need to remove the tissue from the enclosure at any point during the sterilization, seeding, culturing, cryopreservation, shipping, or restoration process. Also disclosed is an apparatus and method of pipette interface with a container in a manner that blocks contaminants from entering the container. | 06-03-2010 |
| 20100151570 | SYSTEMS AND METHODS FOR VITRIFYING TISSUE - An apparatus for vitrifying a biological specimen includes a cap member, a tubular plunger, and a specimen chamber. The cap member includes a stem portion integrally formed with a receiving portion. The receiving portion has a disc-like shape and includes an outer surface. The outer surface includes a first skirt member comprised of a heat-sealable material. The first skirt member is attached along a circumferential portion of the outer surface. The specimen chamber has an open first end portion, a closed second end portion, and a cavity extending between the first and second end portions. The cavity is defined by oppositely disposed inner and outer surfaces. The specimen chamber further includes a second skirt member comprised of heat-sealable material. The second skirt member is attached along a circumferential portion of the outer surface. | 06-17-2010 |
| 20100151571 | CENTIMETER-SCALE, INTEGRATED DIAGNOSTICS INCUBATOR FOR BIOLOGICAL CULTURING - Disclosed are portable, disposable, centimeter-scale, integrated diagnostics incubators for use in biological culturing. An exemplary incubator comprises an optically accessible enclosure having a plurality of fluidic ports. A heating element is disposed within the enclosure that is coupled to an external heater controller. An autoclavable microfluidic perfusion chamber is disposed within the enclosure that comprises a cell culture life support chamber, an inlet port disposed in the perfusion chamber, a collection chamber in communication with the culture chamber, an outlet port coupled to the collection pool, and a perfusing substrate. An optically transparent, gas permeable membrane is attachable to the top of the perfusion chamber. The incubators have optical accessibility, forced flow fluidic control, temperature control, are portable and modular, and are inexpensively manufactured. The incubators permit in-the-field drug testing and culturing of biological tissues. | 06-17-2010 |
| 20100159590 | SYSTEMS AND METHODS FOR ACTIVE MICROFLUIDIC CELL HANDLING - A method and system for providing a cell support system are described. The method and system include providing a first block, a second block coupled to the first block, and a plurality of microfluidic channels. The first block includes a plurality of cell wells therein. The second block includes a plurality of through holes therethrough. The plurality of holes are in fluid communication with at least one corresponding cell well of the plurality of cell wells. The plurality of microfluidic channels are in fluid combination with at least a portion of the plurality of cell wells and are configured to provide an active fluid flow with the portion of the plurality of cell wells. | 06-24-2010 |
| 20100221830 | Device and Method for Ambient Storage of Fresh/Frozen Tissue Sections Via Desiccation - The present invention discloses a method for storing and preserving previously sectioned, snap-frozen, and desiccated biological tissue samples at ambient conditions. Also disclosed are devices and systems for ambient storage of biological tissue sections, and methods for forming said devices. | 09-02-2010 |
| 20100221831 | LIVING BODY HOLDING METHOD, LIVING BODY TEST METHOD, LIVING BODY GROWING METHOD, LIVING BODY HOLDING SHEET, AND LIVING BODY PROCESSING DEVICE - A method of arranging a large number of living bodies, such as cells, embryos, or organisms rapidly, individually, and one by one, a holding sheet for the method, and a device for processing the living bodies. The method of holding living bodies includes using a sheet in which multiple through-holes with a size capable of holding one of the target living bodies, but not capable of holding two or more of the living bodies, are provided, to thereby arrange and hold the living bodies one by one in the multiple through-holes in the sheet together with a liquid. | 09-02-2010 |
| 20100240127 | Method of cryopreserving cells - A non-linear cooling cryopreservation method for improving cryopreservation protocols for cells that involves producing a simulation of cellular responses to a range of cooling parameters; determining optimal cooling parameters required to minimize cryoinjury to the cells using simulation of cellular responses and experimental results; and incorporating optimal parameters into the protocol. The simulation is based on mathematical models of cellular parameters. A non-linear cooling cryopreservation protocol for cryopreserving stem cells is also disclosed that does not require cryoprotectants. | 09-23-2010 |
| 20100248363 | MATRICES AND MEDIA FOR STORAGE AND STABILIZATION OF BIOMOLECULES - The present invention provides compositions useful for biomolecule storage comprising a water soluble inorganic compound, a stabilizer, or a combination thereof. The present invention also provides methods of using the compositions of the invention to store biomolecules in the dry state and in solution, as well as sample carriers and kits comprising compositions of the invention. | 09-30-2010 |
| 20100291679 | COMPOSITION FOR COLLECTING AND PRESERVING PLACENTAL STEM CELLS AND METHODS OF USING THE COMPOSITION - The present invention provides improved compositions and methods for the collection of stem cells from an organ, e.g., placenta. The invention provides a stem cell collection composition comprising an apoptosis inhibitor and, optionally, an enzyme such as a protease or mucolytic enzyme, vasodilator, necrosis inhibitor, oxygen-carrying perfluorocarbon, or an organ preserving compound. The invention provides methods of using the stem cell collection composition to collect stem cells and to preserve populations of stem cells. | 11-18-2010 |
| 20100297759 | SYSTEM AND PROCESS FOR SORTING BIOLOGICAL PARTICLES IN LIQUID FLOW - The embodiment of the present invention is to provide a system for sorting biological particles in a liquid flow, such as a cell sorter, which comprises an optical mechanism emitting laser beam onto each of the biological particles to detect light therefrom, a controller determining a moving speed of each of the biological particles in the liquid flow in accordance with the light detected therefrom, and an electric charger charging each of the biological particles based upon the moving speed thereof. Therefore, the cell sorter according to the embodiment of the present invention can improve recovery rate and purity of the desired cell particles. | 11-25-2010 |
| 20100311165 | DEVICE FOR COLLECTION AND PRESERVATION OF TISSUE OR STOOL SAMPLES - An arrangement for collecting a tissue or stool sample has a sample vial and a source of preservative fluid. A fluid valve controls a flow of the preservative fluid from the source of preservative fluid to the sample vial. An air equalization vent tube is coupled to the source of preservative fluid and an air valve controls a flow of air through the air equalization vent tube, the air valve and the fluid valve being actuated simultaneously. A scoop for collecting the sample is coupled to the sample vial coupler and extends into the sample vial. The preservative fluid flows from the source of preservative fluid to the sample vial by force of gravity upon actuation of the fluid valve. The air equalization vent tube transfers air from the sample vial to the source of preservative fluid upon actuation of the air valve. | 12-09-2010 |
| 20100317107 | METHOD AND DEVICE FOR COLLECTING AND PRESERVING CELLS FOR ANALYSIS - The claimed subject matter comprises a device to collect and preserve cells comprising of: (1) a collection container comprised of a tube having an open end and a closed end, a closure in the open end of the tube, a vacuum drawn to a predetermined level inside the container; and (2) compounds including an anticoagulant agent and a fixative agent, wherein the compounds are in a sufficient amount to preserve said cells” original morphology and antigenic sites without significant dilution of said cells, and thereby allowing said cells to be directly analyzed by a flow cytometer without further treatment. The claimed subject matter further comprises of a method of making a collection device for cells comprising of: (1) providing a tube having an open end and a closed end; (2) preloading compounds including: an anticoagulant agent, and a fixative agent into the tube, wherein the compounds are in a sufficient amount to preserve the cells” original morphology and antigenic sites without significant dilution of the cells, and thereby allowing the cells to be directly analyzed by a flow cytometer without further treatment; (3) inserting a closure into the open end of the tube; and (4) drawing a vacuum inside the tube to a predetermined level to form the collection device. | 12-16-2010 |
| 20100317108 | Cryopreservation of Biological Cells and Tissues - The method involves placing an oocyte cell in a cell holder ( | 12-16-2010 |
| 20100323441 | METHOD OF COLLECTION AND PRESERVATION OF FLUIDS AND/OR MATERIALS, IN PARTICULAR OF ORGANIC FLUIDS AND/OR MATERIALS CONTAINING STEM CELLS, AND DEVICE EMPLOYABLE IN SUCH METHOD - A method of collection and preservation of a fluid and/or a material comprises a step of determining a sampling volume that is closed and/or separated from the external environment, a step of taking a predetermined amount of fluid and/or material from the sampling volume and a step of confining the fluid and/or material into a collection volume; the step of taking the fluid and/or material and the step of confining the fluid and/or material are simultaneous with each other and contemporaneous with a step of maintaining the hydraulic and/or pneumatic and/or microbiological isolation between the sampling volume and the collection volume. | 12-23-2010 |
| 20110014700 | Methods of Storing and Providing Samples of Cells and Tissue - A cell or tissue sample from a donor is stored in bank wherein the donor can initially determine the sample status to (a) exclusively retain, (b) make public, or (c) optionally make public his cells or tissue. Where the donor opts for optional public storage, the donor may in response to a third party request convert the optional public storage status to public status or private status top thereby share or exclusively retain his cells or tissue. Most preferably, depending on the initial and/or subsequent choice by the donor, an incentive or disincentive is provided. | 01-20-2011 |
| 20110014701 | Protection of Progenitor Cells and Regulation of Their Differentiation - The present invention relates to the use of polysulfated polysaccharides in combination with progenitor cells to improve the viability of the progenitor cells including improving the cryopreservation of the progenitor cells and provides novel compositions, methods and uses. The present invention also relates to the use of polysulfated polysaccharides to regulate the proliferation and differentiation of progenitor cells. | 01-20-2011 |
| 20110097796 | METHOD FOR FREEZING AND STORING CELLS - This invention relates to a method for freezing and storing cells in a container, and relates to frozen cells in freezing medium in a container. | 04-28-2011 |
| 20110117647 | Red Blood Cell Storage Medium For Extended Storage - Synthetic aqueous storage solutions are disclosed for use in the processing and the storing of red blood cells prepared from whole blood including cells derived from whole blood held for an extended period at room temperature. | 05-19-2011 |
| 20110151556 | METHODS FOR ISOLATING MONONUCLEAR CELLS THAT INCLUDE A SUBPOPULATION OF MESENCHYMAL PROGENITOR CELLS AND VASCULAR CELLS THAT INCLUDE A SUBPOPULATION OF ENDOTHELIAL PROGENITOR CELLS FROM UMBILICAL CORD TISSUE - Provided herein are methods and kits for the isolation, processing and cryopreservation of mesenchymal cells from the Wharton's Jelly and vascular progenitor cells from umbilical cord tissue. Also provided are isolated mesenchymal cells or vascular progenitor cells obtained by the invention methods, and compositions thereof. | 06-23-2011 |
| 20110159585 | DIRECTLY INJECTABLE FORMULATIONS WHICH PROVIDE ENHANCED CRYOPROTECTION OF CELL PRODUCTS - This invention provides compositions and methods for cryoprotection of recombinant live cancer cells. Specifically, an improved cryoprotective medium is provided which includes a hydroxyethyl starch and/or derivative thereof alone or in combination with either DMSO or glycerol. | 06-30-2011 |
| 20110223662 | COMPOSITION FOR PRESERVING PLATELETS AND METHOD OF USING THE SAME - The present invention relates to compositions and methods for storing platelets to preserve the function and freshness of the platelets. More particularly, the present invention relates to the use of a preservative composition having an antiplatelet agent, an anticoagulant, and an oxygen carrier, for maintaining the freshness of platelets. Additionally, the composition may also contain an ultra-short acting broad spectrum anti-microbial agents. The preservative composition may be used to store platelets in a liquid state, a frozen state, or a freeze-dried state. | 09-15-2011 |
| 20110223663 | COMPOSITION FOR PRESERVING PLATELETS AND METHOD OF USING THE SAME - The present invention relates to compositions and methods for storing platelets to preserve the function and freshness of the platelets. More particularly, the present invention relates to the use of a preservative composition having an antiplatelet agent, an anticoagulant, and an oxygen carrier, for maintaining the freshness of platelets. Additionally, the composition may also contain an ultra-short acting broad spectrum anti-microbial agents. The preservative composition may be used to store platelets in a liquid state, a frozen state, or a freeze-dried state. | 09-15-2011 |
| 20110229965 | Methods of Preparing Mesenchymal Stem Cells, Compositions and Kit Thereof - The present invention discloses a method of isolation, pooling and further culturing of Mesenchymal Stem cells (MSC) for clinical application. Present invention also discloses the method of establishing Master Cell bank, followed by Working Cell Bank from which the final therapeutic composition referred to as Investigational Product/Investigational Medicinal Product comprising of allogenic bone marrow-derived MSC is formulated for clinical applications. | 09-22-2011 |
| 20110275153 | CRYOGENIC STORAGE DEVICE - A cryopreservation device for storing reproductive biological material is provided. The device comprises an elongate first member includes an elongate first member extending between a distal end and a proximal end, a first bulge portion disposed around a circumference of the first member, and a trough defined within the first member, the trough being configured to receive a reproductive biological sample thereon. A second member includes a second member with a lumen defined therethrough. The second member is configured to slide over the first member and the inner diameter of the second member is similar to an outer diameter of the bulge portion to form a seal between the first and second members. | 11-10-2011 |
| 20110281352 | Transplant Storage | 11-17-2011 |
| 20110287534 | AUTOMATED FILLING OF FLEXIBLE CRYOGENIC STORAGE BAGS WITH THERAPEUTIC CELLS - An apparatus and processes for aseptically dispensing live mammalian cells into sterile, flexible bags in a non-sterile atmosphere. The method includes the steps of: providing the cells suspended in a liquid; providing a plurality of sterile flexible bags fluidly connected to a main line by a plurality of branch lines of sterile flexible tubing; evacuating air from the flexible bags by applying a vacuum to the open end of the main line; preventing fluid flow through all branch lines except that of one bag to be filled; dispensing a desired volume of cell suspension into the open end of the main line; and introducing sufficient sterile purging gas under pressure into open end of the main line to drive into the bag any of the dispensed volume remaining in the main line or branch line of filled bag. Cells can be cryogenically preserved in the filled bags. | 11-24-2011 |
| 20110312092 | DUAL-TERMINAL AMIDE HYDROGELLING AGENT - Disclosed is a benzamide derivative represented by formula (1). In the formula, k1 represents an integer from 0-4, m1 represents an integer from 1-100, and n1 represents an integer from 1-6. In addition, R | 12-22-2011 |
| 20120058558 | COMPOSITION FOR PRESERVING PLATELETS AND METHOD OF USING THE SAME - The present invention relates to compositions and methods for storing platelets to preserve the function and freshness of the platelets. More particularly, the present invention relates to the use of a preservative composition having an antiplatelet agent, an anticoagulant, and an oxygen carrier, for maintaining the freshness of platelets. Additionally, the composition may also contain an ultra-short acting broad spectrum anti-microbial agents. The preservative composition may be used to store platelets in a liquid state, a frozen state, or a freeze-dried state. | 03-08-2012 |
| 20120122215 | PLACENTAL STEM CELL POPULATIONS - The present invention provides placental stem cells and placental stem cell populations, and methods of culturing, proliferating and expanding the same. The invention also provides methods of differentiating the placental stem cells. The invention further provides methods of using the placental stem cells in assays and for transplanting. | 05-17-2012 |
| 20120149108 | CELL PRESERVATION METHOD - A method for cryopreservation of cells characterized by the use of a solution containing, as essential components, a sodium salt, a potassium salt, a saccharide, a cryoprotectant, and a hydrogencarbonate salt and/or a carbonate salt, the solution further optionally containing a component selected from the group consisting of proteins, magnesium salts, and calcium salts. According to the present invention, a method for cryopreservation of cells capable of maintaining a high viability rate after thawing, and a cryopreservation solution used in the cryopreservation are provided. In addition, by using the method for cryopreservation, the cells can be preserved in a state of maintaining a high viability rate even after thawing, and also maintaining their physiological functions; further, a cell washing step after freezing and thawing is unnecessary, thereby making it very useful in the fundamental researches of cells and the application studies on the medical treatment. | 06-14-2012 |
| 20120184033 | CONTAINER FOR STORING A FLOWABLE BODILY MATERIAL, AND A METHOD FOR STORING A FLOWABLE BODILY MATERIAL - A container ( | 07-19-2012 |
| 20120184034 | Specimen Preservation Chamber and Method - The present invention comprises a specimen preserver. A bubble reservoir is used to hold a preserving liquid such as artificial cerebrospinal liquid (aCSF). Oxygen is introduced into the aCSF by a gas diffuser which is attached to the bubble reservoir. The tissue samples are contained in one or more chambers in a holding platform. The platform is suspended well above the bottom of the bubble reservoir. The lower portion of each of the chambers is open toward the bottom of the bubble reservoir, but is covered by a fine mesh. The gas introduced into the aCSF circulates within the bubble reservoir. However, the tissue samples are not directly exposed to the gas bubbles because the fine mesh excludes the bubbles from the chambers in which the tissue samples are housed. | 07-19-2012 |
| 20120196362 | METHOD FOR TREATMENT AND STORAGE OF PLATELETS - Provides are improved methods for storing platelets and compositions that contain stored platelets for use in transfusions. The method entails obtaining a platelet concentrate from blood obtained from an individual and holding the platelet concentrate in at refrigerated temperatures under an atmosphere having a pressure of from 3.5 to 5 bars comprising more than 65% xenon and for at least one week. Also provided is a refrigerated composition that contains a platelet concentrate, wherein the platelet concentrate contains xenon, and wherein the platelet concentrate has been isolated from an individual for at least seven days. | 08-02-2012 |
| 20120264211 | CRYOPRESERVATION OF ARTICULAR CARTILAGE - The invention relates generally to methods and compositions for the cryopreservation and/or vitrification of tissue including articular cartilage and the preparation of said tissue for clinical or research use, including but not limited to joint replacement and the treatment and prevention of osteoarthritis. | 10-18-2012 |
| 20120270316 | METHODS AND REAGENTS FOR PRESERVING RNA IN CELL AND TISSUE S AMPLES - This specification relates to the field of molecular biology and provides novel methods and reagents for preserving and protecting the ribonucleic acid (RNA) content of samples from degradation prior to RNA isolation. This preservation may be accomplished without ultra-low temperature storage or disruption of the tissue. | 10-25-2012 |
| 20120301959 | Cryoconserved Mature Dendritic Cells - The invention relates to a method for producing ready to use, antigen loaded or unloaded, cryoconserved mature dendritic cells especially for the production of a vaccine containing said dendritic cells, wherein immature dendritic cells are cultivated in the presence of suitable maturation stimuli and the mature dendritic cells thus obtained are frozen. The dendritic cells can be loaded with antigen before freezing. The invention also relates to a vaccine which can be obtained according to the inventive method and to a composition containing frozen, mature dendritic cells which are loaded with antigen. | 11-29-2012 |
| 20130005036 | MANUFACTURING PROCESS FOR FRESH AND FROZEN STEM CELLS - The present application discloses a method of obtaining multi-lineage stem cells or progenitor cells by allowing a sample of cells to settle in a container; transferring supernatant from the container to another container; and eventually isolating a colony from the supernatant after several transfer/settle processes and expanding further, and optionally freezing the cells thus obtained. | 01-03-2013 |
| 20130059380 | PRESERVATION SOLUTIONS FOR BIOLOGICS AND METHODS RELATED THERETO - The present invention provides compositions (i.e., preservation solutions) for preserving biologies comprising at least one membrane penetrable sugar, at least one membrane impenetrable sugar, at least one anti-microbial agent, at least one anti-oxidant, adenosine, albumin, a salt, a buffer, and a chelating agent, and to methods of using such preservation solutions to preserve biologies. | 03-07-2013 |
| 20130059381 | SOLUTION FOR NON-PROGRAMMED CELL CRYOPRESERVATION - The invention provides a solution for non-programmed cell cryopreservation, which comprises 1.0-28 w/v % of cell membrane protectant, 1.0-18 w/v % of permeable intracellular protectant, 3.0-28 w/v % of cell sedimentation stabilizer, and the balance of solvent. The cell cryopreservation solution provided by the invention is good for protecting cells. After the cryopreservation solution is added to cells, the cells can be cryopreserved in a refrigerator of −80 DEG C. directly without complicated programmed cryopreservation, thus, the time for cell cryopreservation is shortened greatly and the efficiency of cryopreservation is improved. Therefore, the cryopreservation solution is suitable for cryopreserving a large number of cells. The recovery rate of the cells cryopreserved is high, the growth and differentiation of the recovered cells is normal. The components of the cryopreservation solution are stable, thus the cryopreservation solution can be preserved with good property for a long time. | 03-07-2013 |
| 20130065301 | SYSTEMS AND METHODS FOR CRYOPRESERVATION OF CELLS - An auto-nucleating device includes a tube containing a crystalline cholesterol matrix. The ends of the tube are closed by a membrane impermeable to the cholesterol but permeable to liquids contained in a cryopreservation vessel. The auto-nucleating device provides a site for ice nucleation during freezing of the liquid within the vessel. The cryopreservation vessel can be a flexible vial having a closed port adapted to be pierced by a needle, and an opposite end that is initially open to receive the liquid. Another vessel includes an adaptor mounted to liquid container with a tubular branch closed by a needle septum and another tubular branch provided with a barbed fitting for engaging a flexible tube that terminates in a needle septum. Alternatively, the vessel includes an inlet and vent branch at the top of the container and an outlet septum at a bottom opening. | 03-14-2013 |
| 20130065302 | UMBILICAL CORD LINING STEM CELLS AND METHODS AND MATERIAL FOR ISOLATING AND CULTURING SAME - Human umbilical cord lining stem cells that are capable of differentiating into cells of the mesodermal lineage and ectodermal lineage are described, as well as methods of isolating, expanding, culturing, and cryopreserving such cells. | 03-14-2013 |
| 20130157362 | METHOD OF LIQUID NITROGEN SURFACE VITRIFICATION - A method of liquid nitrogen surface vitrification requiring an embryo washed in a rinsing medium, then incubated in a base medium and incubated in a hold medium before being washed in a vitrification medium and produced into a vitrification droplet ( | 06-20-2013 |
| 20130210142 | Methods and Compositions for Enhancing Vascular Access - Disclosed is an implantable material comprising a biocompatible matrix and cells which, when provided to a vascular access structure, can promote functionality generally. For example, implantable material of the present invention can enhance maturation of an arteriovenous native fistula as well as prolong the fistula in a mature, functional state suitable for dialysis. Additionally, the present invention can promote formation of a functional arteriovenous graft suitable for dialysis as well as promote formation of a functional peripheral bypass graft. Implantable material can be configured as a flexible planar form or a flowable composition with shape-retaining properties suitable for implantation at, adjacent or in the vicinity of an anastomoses or arteriovenous graft. According to the methods disclosed herein, the implantable material is provided to an exterior surface of a blood vessel. Certain embodiments of the flexible planar form define a slot. The materials and methods of the present invention comprise cells, preferably endothelial cells or cells having an endothelial-like phenotype. | 08-15-2013 |
| 20130210143 | NOVEL METHODS FOR PREVENTING OR TREATING DIABETES - The present application relates to an LRH-1 agonist for use in the prevention of progressive loss of pancreatic β-cells. It also relates to an LRH-1 agonist for use in the preservation or restoration of pancreatic β-cells. Further, it relates to an LRH-1 agonist for use in the prevention or treatment of type I diabetes, the increment of survival of pancreatic β-cells, the increment of the performance of pancreatic β-cells, the increment of the survival of a β-cell graft, the in vitro preservation of pancreatic β-cells, maintaining insulin secretion and/or in a method of transplanting pancreatic islet cells. | 08-15-2013 |
| 20130217123 | METHOD FOR DENTAL PULP CRYOPRESERVATION - The present invention relates to a method for cryopreserving the pulp of a non-exfoliated deciduous tooth, comprising a step of making with a laser a hole into the tooth removed from its physiological seat on the tooth neck. After making the hole, the tooth is contacted with a cryopreserving agent and then cryofrozen. An object of the invention further consists in mesenchymal stem cells isolated from the pulp of a cryopreserved tooth according to the method of the invention. | 08-22-2013 |
| 20130260459 | Methods for Preserving Target Cells - Methods for obtaining and preserving target cells using degradable three dimensional matrices are described. | 10-03-2013 |
| 20130273654 | Generation of Dendritic Cells from Monocytic Dendritic Precursor Cells with GM-CSF in the Absence of Additional Cytokines - The present invention it was determined that dendritic cells could be derived from various sources including peripheral blood monocytes in the presence of only GM-CSF without other cytokines if the monocytes were not activated. By preventing activation, such as by preventing binding of the cells to the surface of the culture vessel, the monocytes do not require the presence of additional cytokines, such as IL-4 or IL-13, to prevent differentiation into a non-dendritic cell lineage. The immature DCs generated and maintained in this manner were CD14 | 10-17-2013 |
| 20130337563 | ISOLATION, CULTIVATION AND USES OF STEM/PROGENITOR CELLS - The present invention relates to a method for isolating stem/progenitor cells from the amniotic membrane of umbilical cord, wherein the method comprises separating the amniotic membrane from the other components of the umbilical cord in vitro, culturing the amniotic membrane tissue under conditions allowing cell proliferation, and isolating the stem/progenitor cells from the tissue cultures. The isolated stem cell cells can have embryonic stem cell-like properties and can be used for various therapeutic purposes. In one embodiment, the invention relates to the isolation and cultivation of stem cells such as epithelial and/or mesenchymal stem/progenitor cells under conditions allowing the cells to undergo mitotic expansion. Furthermore, the invention is directed to a method for the differentiation of the isolated stem/progenitor cells into epithelial and/or mesenchymal cells. | 12-19-2013 |
| 20140017785 | Methods for Collecting and Processing Autografts, Processed Autografts, Kits for Collecting and Transporting Autografts, and Tools for Preparing Autografts - The present invention is directed to methods for collecting and processing autografts, processed autografts, kits for collecting and transporting autografts, and tools for preparing autografts. It is also directed to autologous bone grafts, and methods of preparing them. | 01-16-2014 |
| 20140051165 | STEM CELL PACKAGING AND SHIPPING - This invention concerns methods of packaging and shipping stem cells. Also disclosed are related package products. | 02-20-2014 |
| 20140113371 | Cryopreservation of Haptenized Tumor Cells - A method of preserving haptenized tumor cells is described. The method employs a freezing medium containing an effective amount of sucrose and human serum albumin in an isotonic buffered saline solution. Cryogenically preserving haptenized cells in such a medium has been found to maintain the integrity of the tumor cells during storage. The haptenized tumor cells also retain cell-associated antigens and haptens, and are as immunogenic, i.e., capable of inducing immunotherapeutic response, as fresh vaccine in a mouse model of metastatic disease. In a specific embodiment, haptenized cells are exposed to a solution of 8% sucrose, 10% human serum albumin in Hank's buffered solution, and then frozen to −80° C. overnight and then stored in a liquid nitrogen freezer. Methods of storing haptenized tumor cells and compositions are also provided. | 04-24-2014 |
| 20140170749 | MULTIPOTENT STEM CELLS AND USES THEREOF - The invention provides a quiescent stem cell having the capacity to differentiate into ectoderm, mesoderm and endoderm, and which does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CD34, CD49c, CD73, CD105, CD90, CD66A, CD66E, CXCR4, CD133 or an SSEA. The invention further provides a proliferative stem cell, which expresses genes including Oct-4, Nanog, Sox2, GDF3, P16INK4, BMI, Notch, HDAC4, TERT, Rex-1, TWIST, KLF-4 and Stella but does not express cell surface markers including MHC class I, MHC class II, CD44, CD45, CD13, CF34, CF49c, CD73, CD105, CD90, CD66A, CD66E, CXCR4, CD133 or an SSEA. The cells of the invention can be isolated from adult mammals, have embryonic cell characteristics, and can form embryoid bodies. Methods for obtaining the stem cells, as well as methods of treating diseases and differentiated stem cells, are also provided. | 06-19-2014 |
| 20140220684 | COMPOSITION COMPRISING OXYGEN-RELEASING MICROCAPSULES CONTAINING HYDROGEN PEROXIDE FOR ENHANCING CELL PROTECTION AND VIABILITY AT ROOM TEMPERATURE - The present invention provides a composition comprising oxygen-releasing microcapsules containing hydrogen peroxide for protection of cells at room temperature. The oxygen-releasing microcapsules containing hydrogen peroxide according to the present invention has an excellent effect of improving the viability of cells at room temperature and thus can be effectively used as a composition for protection of cells in manipulating, storing, or transporting cells at room temperature. | 08-07-2014 |
| 20140287501 | HUMAN DISC TISSUE - This invention provides disc stem cells, processes for obtaining and culturing disc stem cells, and methods for repairing damaged or diseased disc tissue comprising the use of the disc stem cells of the invention. | 09-25-2014 |
| 20140287502 | METHOD FOR PRODUCING CELL CONCENTRATE - The present invention provides a method that allows the preparation of a cell concentrate in a short time without loss of cells and great damage on cells by simple operations. The present invention provides a method for producing a cell concentrate using an inside-out filtration system for processing a cell suspension, the system including: a cell suspension inlet port; a filtrate outlet port; a cell suspension outlet port; and a hollow fiber separation membrane interposed between the cell suspension inlet port and the cell suspension outlet port, wherein the hollow fiber separation membrane is provided with inner pores with an average pore size of 0.1 μm to 10 μm, and the quotient of the division of an initial filtrate flow rate by a linear velocity of the cell suspension flowing through the hollow fibers is 2.5 or less. | 09-25-2014 |
| 20140302602 | FILM-SHAPED TISSUE STORAGE TRANSPORT CONTAINER AND STORAGE TRANSPORT METHOD - A film-shaped tissue storage transport container and storage transport method. The storage transport container includes: an external container; a coupling mechanism which couples a container main body and a lid member of the external container; an internal container which is positioned within the external container; an interposed unit having elasticity and which is sandwiched between the internal container and the lid member; and a storage fluid with which the internal container is filled. A film-shaped tissue is present in a floating state in the storage fluid with which the internal container is filled. | 10-09-2014 |
| 20140335614 | METHOD AND DEVICES FOR CRYOPRESERVATION OF BIOMATERIALS - Improved methods for cryopreservation of cells result when cells are cooled at controlled rates in devices that ensure a constant rate of cooling of less than 10° C. | 11-13-2014 |
| 20140342454 | DEVICE FOR VITRIFICATION AND/OR REANIMATION OF OOCYTES, EMBRYOS OR BLASTOCYSTS - Disclosed herein are devices, methods, systems and kits adapted for vitrification and/or reanimation of oocytes, embryos or blastocysts. The device includes a straw and a filter, wherein the straw comprises a lumen traversing through the straw and has a proximal section, a middle section and a distal section and wherein the filter is affixed in the straw and comprises a plurality of pores having a diameter smaller than the diameter of said oocytes, embryos or blastocysts but large enough to allow the passage of a fluid composition therethrough. | 11-20-2014 |
| 20140356953 | DISPENSING UNIT FOR DISPENSING PRESERVATION FLUID INTO A TISSUE SAMPLE CONTAINER - The invention relates to a dispensing unit for dispensing fluid into an external container. The dispensing unit comprises: a connector, a reagent cylinder for storing the fluid, and a locking ring. The connector comprises an upper fastener, and a bottom wall with at least one passage therethrough. The reagent cylinder comprises at least one compartment for storing the fluid, which compartment comprises at least one opening through a cylinder bottom. The at least one opening is located so that for at least one mutual orientation of the reagent cylinder and the connector, fluid can flow from the compartment to the external container via the at least one opening and the at least one passage. The locking ring comprises a second fastener which fastens to the upper fastener of the connector, so that when the locking ring is tightened, the reagent cylinder is pressed towards the interior bottom of the connector. | 12-04-2014 |
| 20150011000 | Cryopreservation of Biological Cells and Tissues - The method involves placing an oocyte cell in a cell holder ( | 01-08-2015 |
| 20150024487 | CRYOPRESERVATION STORAGE DEVICE FOR CELL COLLECTION BAG, AND USING METHOD THEREOF - The present invention relates to a cryopreservative device comprising an outer case, one or more layers of space for the cryo-bags, and two or more Teflon cryopreservative bags. The outer case has a cover lip for opening and closing. The Teflon cryopreservative bags are filled with a freezing resistant. In the present invention, the cryo-bags and the Teflon cryopreservative bags are crossly stacked in the cryopreservation device. The Teflon cryopreservative bags are designed to directly contact with the cryo-bags in order to obtain the effect of slow cell freezing. | 01-22-2015 |
| 20150072423 | Cell Preparation Method - A method of thawing cryopreserved human hepatocytes and cryopreserving the hepatocytes a second time without losing viability is described. It allows the preparation of cryopreserved human hepatocytes pooled from multiple donors by thawing the hepatocytes from the individual donors, combining the cells to form pooled hepatocytes, and recryopreserving the pooled hepatocytes. The method involves the thawing of the hepatocytes from the individual donors, maintaining the thawed hepatocytes at a low temperature that is above freezing temperature (e.g., 4° C.), and refreezing the thawed cells without further manipulation. The method allows the cryopreserving the human hepatocytes a second time after thawing, with viability similar to that after one single cryopreservation. This high efficiency method can be used for the preparation of highly viable pooled human hepatocytes for experimentation to minimize individual variations. | 03-12-2015 |
| 20150072424 | CRYOGENIC COOLING THIN FILM EVAPORATOR - One aspect of the present invention is directed to an apparatus for cryogenically cooling a substance. The apparatus of the present invention includes a thin film evaporator comprising a microstructured surface and an applicator for dispensing a working fluid onto the microstructured surface. The apparatus preferably contains a pressure-controlled vessel enclosing the thin film evaporator and at least a portion of the applicator. In a second aspect, the present invention is directed to the method of using a thin film evaporator of the present invention to cryogenically cool a substance by forming a thin film layer of the working fluid on the microstructured surface of the thin film evaporator. | 03-12-2015 |
| 20150140656 | METHOD OF FREEZING CELLS - The present invention is a method of freezing cells comprising the steps of incubating said cells in a solution comprising a cryoprotective agent, concentrating the cells resulting from the previous step withdrawing the eluent essentially free of cells, and freezing the resulting concentrated cells. The cells frozen by the invention's method render a high post-thawing viability, reduce cryoprotectant related toxic events and promote cells life in a suspension state after thawing. The invention also comprises the container comprising the frozen cells. | 05-21-2015 |
| 20150313211 | A Method of Vitrification - The present invention relates to a vitrification method. In particular, the present invention relates to a method of producing at least one vitrified cell comprising loading a cell into a holding space in at least one conduit; providing at least one cryoprotectant to the holding space of the conduit in increasing concentrations, wherein the cryoprotectant is capable of equilibrating the cell; cooling the cell in the holding space of the conduit to produce a vitrified cell; and storing and maintaining the vitrified cell in the holding space of the conduit. | 11-05-2015 |
| 20150327537 | COMPOSITION COMPRISING PLANT-DERIVED RECOMBINANT HUMAN SERUM ALBUMIN, LIPIDS, AND PLANT PROTEIN HYDROLYSATES AS ACTIVE INGREDIENTS FOR CRYOPRESERVATION OF STEM CELLS OR PRIMARY CELLS - The present invention relates to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, and use thereof, more particularly, to a composition including plant-derived recombinant human serum albumin, lipids, and plant protein hydrolysates as active ingredients for cryopreservation of stem cells or primary cells, in which the albumin, lipids, and plant protein hydrolysates are present in sufficient quantities to reduce cell death caused by freezing while maintaining animal-free and xeno-free conditions during long-term preservation of stem cells or primary cells; and to a method for cryopreservation of stem cells or primary cells using the composition. | 11-19-2015 |
| 20150342174 | COMPOUNDS FOR PROTECTION OF CELLS - This invention is related to a compound with the structural formula (I) | 12-03-2015 |
| 20150351381 | Method, System and Apparatus for Improved Micromanipulation and Storage - The present application relates to the manipulation and handling of biological materials and, in one form, provides an apparatus for micromanipulation of biological material, including a channel for accommodating biological material and allowing for passage of liquid treatment solutions. The apparatus may include a two part construction wherein two portions of the apparatus are adapted to be heat sealed with a secondary material intermediate the two portions prior to a vitrification process step. A system for vitrification of a biological specimen is also provided including a software operable means for controlling the temperature environment, a software operable means for controlling fluid dispense volume and velocity and aspiration volume and velocity for the application of liquid treatment solutions to the biological specimen, and a software operable means for controlling protocol time. | 12-10-2015 |
| 20150376567 | MEDIA FOR STEM CELLS - The present invention relates to a medium for storing, preserving, culturing, and/or differentiating stem cells. The medium employs a synthetic thermo-responsive copolymer, dispersed in an aqueous vehicle, which allows the medium to be readily “switched” between a non-gelatinous (fluid) form and a gelatinous form by simply adjusting temperature across the gelling temperature of the medium. As such, stem cells can be easily captured within a 3D gel matrix by simply mixing the stem cells with the non-gelatinous/fluid form of the medium and then gelling the medium by adjusting the temperature across the gelling temperature of the medium. The stem cells encapsulated within the gelatinous form of the medium may then be preserved for a significant time period before either using the stem cells directly within the medium (e.g. in therapy, or in culturing, differentiation, and such like), or after their extraction from the medium. | 12-31-2015 |
| 20160000063 | COMPOSITION AND METHOD FOR PRESERVING, TRANSPORTING AND STORING LIVING BIOLOGICAL MATERIALS - Compositions and methods for preserving, transporting and/or storing natural and bioengineered living biological materials represent significant improvements over traditional compositions and methods for preservation, transport and/or storage of biological material by the virtue of their ability to significantly prevent and minimize loss of functional and physical (cell, tissue, and/or organ) integrity to occur in the biological material being preserved, transported and/or stored, particularly at temperatures above freezing point. The compositions and methods are also highly suitable for use in cosmetic procedures, in particular in transplantation of biological materials, especially for transplantation of autologous or allogeneic biological materials. | 01-07-2016 |
| 20160002601 | METHODS OF UPSCALING MESENCHYMAL STROMAL CELL PRODUCTION, COMPOSITIONS AND KIT THEREOF - The present invention discloses a method of isolation, pooling and further culturing of Mesenchymal Stem cells (MSC) for clinical application. Present invention also discloses the method of establishing Master Cell bank, followed by Working Cell Bank from which the final therapeutic composition referred to as Investigational Product/Investigational Medicinal Product comprising of allogenic bone marrow-derived MSC is formulated for clinical applications. Present disclosure also discloses a robust manufacturing process for consistent production of clinical grade Mesenchymal Stromal cells (MSCs). The process enables production of highly viable potent cells. The process steps relating to preparation of media, cell seeding, harvesting are fine tuned to achieve consistency in cell yield, superior cell viability, purity, improved cell proliferation, high cell recovery, low HLA-DR expression, reduction in culture duration. The viability and purity of cells are further improved by optimized wash process without cell loss/cell stress. The disclosure further provides a method of cyrostoring MSCs at high cell density without affecting the viability of cells. It further provides economical means to store and transport at −80° C. | 01-07-2016 |
| 20160021873 | STEM CELL PRESERVATION MEDIUM, STEM CELL PRESERVATION METHOD, AND STEM CELL PRESERVATION SYSTEM - Provided are a stem cell preservation medium, stem cell preservation method and stem cell preservation system which can be used in a slow-freezing method that is used in place of vitrification, have a high cell survival rate and are convenient and highly efficient. The stem cell preservation medium comprises hydroxyethyl starch (HES), dimethyl sulfoxide (DMSO), and ethylene glycol (EG). The stem cell preservation method comprises: a dissociation step in which stem cells are dissociated using a pronase solution; and a freezing step in which the dissociated stem cells are slow-frozen in the stem cell preservation medium. The stem cell preservation system comprises: the pronase solution which is a dissociation means for dissociating stem cells; the stem cell preservation medium according to the present invention; and a slow-freezing means with which the dissociated stem cells are slow-frozen in the stem cell preservation medium. | 01-28-2016 |
| 20160021874 | METHODS AND DEVICES FOR ACHIEVING HIGH SURVIVALS OF LIVING CELLS AFTER FREEZING OR VITRIFICATION - Methods are described for warming a sample that contains a cell or tissue, wherein the method heats the sample at a warming rate of at least about 1×10 | 01-28-2016 |
| 20160022420 | Self-Adjusting Tissue Holder - Tissue holders that can be used for gripping natural or synthetic heart valves are described. The tissue holder can include a clamping mechanism and a spring and can be self-adjusting with regard to pressure applied to the tissue gripped in the holder. The tissue holder can be removably attached to systems for processing the tissues and can provide completely hands-free processing of a tissue from development or excisement to implantation and/or completion of testing. | 01-28-2016 |
| 20160024466 | ISOLATION, CULTIVATION AND USES OF STEM/PROGENITOR CELLS - The present invention relates to a method of cultivating stem/progenitor cells of the amniotic membrane of umbilical cord that comprises obtaining a tissue explant from the amniotic membrane of umbilical cord and cultivating the tissue explant in suitable cultivation media and cultivation conditions over a suitable period of time. The method may include isolating the stem/progenitor cells from the tissue cultures. The stem cells may be epithelial or mesenchymal stem/progenitor cells. The isolated stem cell cells can have embryonic stem cell-like properties and can be used for various therapeutic purposes. | 01-28-2016 |
| 20160029619 | SYSTEM AND METHODS FOR AUTOMATED VITRIFICATION OF BIOLOGICAL MATERIALS - The present invention relaters to a system and methods for automated vitrification of mammalian oocytes or embryos. The system and methods enable automated processing of oocytes or embryos in vitrification solutions; robotically moving vitrification devices that carry processed cells for freezing in liquid nitrogen; automated sealing of the frozen devices; and transferring the sealed devices to an automated storage system for long-term cryopreservation. | 02-04-2016 |
| 20160040117 | Methods and an Apparatus for Transporting Live Cells - The invention provides improved methods and an apparatus for transporting a starter culture of living cells including fungi, bacteria, animal and plant cells. The methods and apparatus enable on-demand feeding and on-demand supply of nutrient gas resulting in increased cell viability and improved fermentations. | 02-11-2016 |
| 20160053228 | ISOLATION, EXPANSION AND CHARACTERIZATION OF PRECURSOR/STEM CELLS FROM DENTAL TISSUES - A method for isolating and proliferating at least one type of precursor cell from dental origin from a single donor includes the following isolating the precursor cells from dental origin of a single donor in a sample collection media and preparing a primary stock culture. The primary stock culture is proliferated sequentially to obtain first, second and third sub-cultured stocks with cell counts ranging between 5×10 | 02-25-2016 |
| 20160057991 | VITRIFICATION-CRYOPRESERVATION IMPLEMENT FOR CELLS OR TISSUES - The present invention relates to a jig for vitrification cryopreservation of cell or tissue, having a metallic support, and a vitrification solution absorber including an adhesion layer and a vitrification solution-absorbing layer on the metallic support in the order from the side closer to the metallic support. | 03-03-2016 |
| 20160066566 | CRYOPRESERVATION OF UMBILICAL CORD TISSUE STRIPS FOR CORD TISSUE-DERIVED STEM CELLS - A method for cryopreserving an umbilical cord tissue strip includes the steps of: (a) applying tension to a segment of an umbilical cord to expand spaces between umbilical cord vessels; (b) cutting the segment of the umbilical cord, at the spaces between the umbilical cord vessels in a longitudinal direction that parallels a length of the umbilical cord vessels, to obtain the umbilical cord tissue strip, wherein the umbilical cord tissue strip contains Wharton's jelly from a perivascular region, a intervascular region, and a subamnion region; (c) incubating the umbilical cord tissue strip with a cryogenic composition; and (d) cryopreserving the umbilical cord tissue strip containing the Wharton's jelly and the cryogenic composition. | 03-10-2016 |
| 20160076982 | AUTOMATED VITRIFICATION DEVICE - The present application relates to devices for the vitrification of biological samples, including embryos, oocytes and biological tissues. Suitably, the devices are automated devices which require little if any operator intervention or sample handling, thereby reducing contamination, improving success rates and increasing efficiency. Also provided are methods of vitrifying a biological sample, suitably using the disclosed devices. | 03-17-2016 |
| 20160095307 | METHOD AND COMPOSITION FOR HYPOTHERMIC STORAGE OF PLACENTAL TISSUE - A tissue storage solution includes hypothermic storage compositions and methods. The hypothermic storage composition includes media containing Dulbecco's modified Eagle's medium (DMEM) and albumin. A method of hypothermically storing tissue includes storing such tissue in a storage medium including DMEM and albumin. A method for wound or defect treatment includes applying tissue, stored in a hypothermic storage medium containing DMEM and albumin, to the site of such wound or defect. | 04-07-2016 |
| 20160102286 | Methods For The Cryopreservation Of Mammalian Cells - Manufactured capillary tubes can include: a tubular member; and a coating applied partially covering the tubular member, the coating defining a window where the tubular member is free of the coating. Kits containing and methods related to the capillary tubes can be used for the cryopreservation of cells. | 04-14-2016 |
| 20160116382 | TISSUE PIECE TREATING APPARATUS AND METHOD OF OPERATING TISSUE PIECE TREATING APPARATUS - The present invention addresses the problem of providing a technology capable of automatically preparing a mixed liquid having a fixed ratio. To address the problem, the following processes are carried out in a tissue piece treating apparatus ( | 04-28-2016 |
| 20160120172 | Cryopreservation device for biological material - A cryopreservation device for biological material comprises an outer tube ( | 05-05-2016 |
| 20160143268 | METHOD AND DEVICE FOR COLLECTING AND PRESERVING CELLS FOR ANALYSIS - The claimed subject matter comprises a device to collect and preserve cells comprising of: (1) a collection container comprised of a tube having an open end and a closed end, a closure in the open end of the tube, a vacuum drawn to a predetermined level inside the container; and (2) compounds including an anticoagulant agent and a fixative agent, wherein the compounds are in a sufficient amount to preserve said cells” original morphology and antigenic sites without significant dilution of said cells, and thereby allowing said cells to be directly analyzed by a flow cytometer without further treatment. The claimed subject matter further comprises of a method of making a collection device for cells comprising of: (1) providing a tube having an open end and a closed end; (2) preloading compounds including: an anticoagulant agent, and a fixative agent into the tube, wherein the compounds are in a sufficient amount to preserve the cells” original morphology and antigenic sites without significant dilution of the cells, and thereby allowing the cells to be directly analyzed by a flow cytometer without further treatment; (3) inserting a closure into the open end of the tube; and (4) drawing a vacuum inside the tube to a predetermined level to form the collection device. | 05-26-2016 |
| 20160165879 | Compositions and Methods for Preservation of Materials in the Amorphous State | 06-16-2016 |
| 20160174544 | Compositions and Methods for Stabilizing Circulating Tumor Cells | 06-23-2016 |
| 20160192640 | SYSTEM AND METHOD TO DIVIDE LIPOSUCTION FAT INTO ALIQUOTS TO BE USE AND CRYOPRESERVED | 07-07-2016 |
| 20160194609 | ISOLATION, EXPANSION AND CHARACTERIZATION OF WHARTON'S JELLY MESENCHYMAL STEM CELLS | 07-07-2016 |
| 20170232086 | CRYOPRESERVATION OF APOPTOTIC CANCER CELLS FOR USE IN IMMUNOTHERAPY AGAINST CANCER | 08-17-2017 |
