Class / Patent application number | Description | Number of patent applications / Date published |
435368000 | Nervous system origin or derivative | 40 |
20080206865 | Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells - A method of differentiating embryonic stem cells into neural and motor cells is disclosed. In one embodiment, the invention comprises culturing a population of cells comprising a majority of cells that are characterized by an early rosette morphology and are Sox1 | 08-28-2008 |
20080213888 | NEURAL PRECURSOR CELLS, METHOD FOR THE PRODUCTION AND USE THEREOF IN NEURAL DEFECT THERAPY - The invention relates to isolated and purified neural precursor cells, to methods for the generation of such precursor cells in unlimited quantities from embryonic stem cells, and to their use for the therapy of neural defects, particularly in mammals, preferably in human beings, and for the generation of polypeptides. | 09-04-2008 |
20080248568 | Directed Neural Differentiation - Differentiation towards a neural fate, and away from a non-neural fate, is promoted by activation of Notch signalling in ES cells and then transferring the cells into neural differentiation protocols. Media for neural differentiation comprises a Notch activator, e.g. a notch ligand that can be clustered. Genetic manipulation is used as an alternative to media additives for Notch activation. | 10-09-2008 |
20100003751 | Methods of generating glial and neuronal cells and use of same for the treatment of medical conditions of the cns - A method of generating neural and glial cells is provided. The method comprising growing human stem cells under conditions which induce differentiation of said human stem cells into the neural and glial cells, said conditions comprising the presence of retinoic acid and an agent capable of down-regulating Bone Morphogenic Protein activity. | 01-07-2010 |
20100086998 | Enriched Central Nervous System Stem Cell and Progenitor Cell Populations, and Methods For Identifying, Isolating and Enriching For Such Populations - Enriched neural stem and progenitor cell populations, and methods for identifying, isolating and enriching for neural stem cells using reagent that bind to cell surface markers, are provided. | 04-08-2010 |
20100159589 | NESTIN-EXPRESSING HAIR FOLLICLE STEM CELLS - Hair follicle stem cells are isolated by virtue of understanding their location within the hair follicle during telogen phase. | 06-24-2010 |
20110014695 | METHODS FOR OBTAINING ADULT HUMAN OLFACTORY PROGENITOR CELLS - An isolated human olfactory stem cell can be prepared by culturing human tissue from olfactory neuroepithelium to form neurospheres. | 01-20-2011 |
20110165674 | NK-1 Receptor Mediated Delivery of Agents to Cells - Provided herein are conjugates including a targeting vehicle coupled to an agent. The targeting vehicle includes a tachykinin receptor ligand and a reactive moiety. Conjugates including a tachykinin receptor ligand attached to an antibody or fragment thereof that is specific for an intracellular target are also provided. Also provided are methods of delivering agents to cells expressing tachykinin receptors, methods of delivering antibodies or fragments thereof to an intracellular extra-endosomal target, and methods of arresting cell growth or introducing cell death of a cancer cell. | 07-07-2011 |
20110171731 | COMPOSITIONS CONTAINING PLATELET CONTENTS - This document provides methods and materials relating to platelet lysates. For example, methods and materials for using platelet lysate compositions to grow adult stem cells, to differentiate adult stem cells, to grow primary cell cultures, to grow tumor stem cells, and to identify effective growth factors are provided. | 07-14-2011 |
20110207215 | CELL STORAGE METHOD AND CELL TRANSPORT METHOD - To provide a cell storage method to store living cells while maintaining the functions of the living cells. An aspect of the cell storage method is a method to store living cells ( | 08-25-2011 |
20110217774 | Efficient and Universal Method for Neural Differentiation of Pluripotent Stem Cells - The present invention relates to a method for inducing neural differentiation of stem cells. In more detail, the present invention relates to a method for inducing neural differentiation of stem cells by inhibiting both BMP (bone morphogenetic protein) and Activin/Nodal signaling pathways in the stem cells. The present invention allows all types of stem cells to effectively differentiate into neural precursor cells regardless of conventional methods for stem cell differentiation including floating culture and attachment culture. In addition, since the neural precursor cells induced by the present invention may be differentiated into specific cells (e.g., dopaminergic neurons) or oligodendrocytes in higher efficient manner, they may be applied to treatment of incurable nerve diseases (e.g., Parkinson's disease or spinal cordinjury) and further provide fundamental data on new drug development. | 09-08-2011 |
20110275152 | Method of in vitro differentiation of neural stem cells, motor neurons and dopamine neurons from primate embryonic stem cells - A method of differentiating embryonic stem cells into neural and motor cells is disclosed. In one embodiment, the invention comprises culturing a population of cells comprising a majority of cells that are characterized by an early rosette morphology and are Sox1 | 11-10-2011 |
20110312091 | PLURIPOTENT STEM CELLS, METHOD FOR PREPARATION THEREOF AND USES THEREOF - Human pluripotent stem cells which are isolated from cut human umbilical cord or placenta and characteristic of cell surface marker CD151 | 12-22-2011 |
20120058556 | High Surface Area Substrate for Cell Culture - A cell culture microcarrier includes (1) a polystyrene microcarrier base having a remnant of a carboxylic acid group, and (ii) a polypeptide conjugated to the base via the remnant of the carboxylic acid group. The polypeptide may contain a cell adhesive sequence, such as RGD. Cells cultured with such microcarriers exhibit peptide-specific binding to the microcarriers. | 03-08-2012 |
20120094381 | METHODS OF NEURAL CONVERSION OF HUMAN EMBRYONIC STEM CELLS - The present invention relates generally to the field of cell biology of stem cells, more specifically the directed differentiation of pluripotent or multipotent stem cells, including human embryonic stem cells (hESC), somatic stem cells, and induced human pluripotent stem cells (hiPSC) using novel culture conditions. Specifically, methods are provided for obtaining neural tissue, floor plate cells, and placode including induction of neural plate development in hESCs for obtaining midbrain dopamine (DA) neurons, motorneurons, and sensory neurons. Further, neural plate tissue obtained using methods of the present inventions are contemplated for use in co-cultures with other tissues as inducers for shifting differentiation pathways, i.e. patterning. | 04-19-2012 |
20120122211 | GENERATION OF NEURAL STEM CELLS FROM UNDIFFERENTIATED HUMAN EMBRYONIC STEM CELLS - The present invention relates to the generation of neural cells from undifferentiated human embryonic stem cells. In particular it relates to directing the differentiation of human ES cells into neural progenitors and neural cells and the production of functioning neural cells and/or neural cells of a specific type. The invention also includes the use of these cells for the treatment of neurological conditions such as Parkinson's disease. | 05-17-2012 |
20120190108 | NEURONAL PROGENITOR CELLS AND METHODS OF DERIVATION AND PURIFICATION OF NEURONAL PROGENITOR CELLS FROM EMBRYONIC STEM CELLS - The invention provides neuronal progenitor cells, populations and cultures of cells, cell compositions and methods of producing neuronal progenitor cells. Neuronal progenitor cells can be prepared from embryonic stem cells, such as human embryonic cells. | 07-26-2012 |
20120295348 | METHOD FOR PROLIFERATING STEM CELLS BY ACTIVATING C-MET/HGF SIGNALING AND NOTCH SIGNALING - The present invention relates to stem cells in which a gene that activates signaling is introduced and to a method for proliferating the stem cells. More specifically, the invention relates to a method of significantly increasing the ability of stem cells to proliferate, either by transfecting stem cells with the Notch intracellular domain (NICD) to activate the Notch signaling pathway, or by transfecting stem cells with the c-MET gene and treating the transfected stem cells with the HGF ligand protein to activate the c-MET/HGF signaling pathway. According to the present invention, as a result of activating the Notch signaling pathway or the c-MET/HGF signaling pathway, stem cells having an excellent ability to proliferate can be produced in large amounts. Particularly, since neural stem cells which have been difficult to culture in vitro can be proliferated in large amounts, thus the neural stem cells will be more useful for the preparation of cell therapeutic agents for treating cranial nerve diseases. | 11-22-2012 |
20130017599 | METHOD OF DIMINISHING THE SYMPTOMS OF NEURODEGENERATIVE DISEASE - A method of diminishing the symptoms of neurodegenerative disease in a patient is disclosed. In one embodiment, the method comprises the steps of: (a) identifying a patient with a neurodegenerative disease, (b) producing a cell culture, wherein the cell culture comprises cells with induced antioxidant response element (ARE) mediated transcription, and (c) transplanting at least a portion of the cell culture into the brain of the patient, wherein symptoms of neurodegenerative disease are diminished. | 01-17-2013 |
20130089926 | METHOD FOR DIFFERENTIATING HUMAN NEURAL PROGENITOR CELLS INTO DOPAMINERGIC NEURONS, AND MEDIUM FOR DIFFERENTIATION THEREOF - The present invention provides a method for differentiating human neural progenitor cells into dopaminergic neurons, comprising the step of culturing human neural progenitor cells in a medium containing fusaric acid. In addition, the present invention provides a medium for differentiation of human neural progenitor cells into dopaminergic neurons. | 04-11-2013 |
20130236963 | Cells Useful for Immuno-Based Botulinum Toxin Serotype A Activity Assays - The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines. | 09-12-2013 |
20130252330 | HOMOZYGOUS AND HETEROZYGOUS IDH1 GENE-DEFECTIVE HUMAN ASTROCYTOMA CELL LINES - We provide IDH1 gene-defective cell lines (e.g., IDH1R132H heterozygous and IDH1R132H homozygous) derived from dissociated human astrocytoma samples. The cells can be used alone or in combination with each other or other cell types as a tool for determining the impact of IDH1R132H on cellular biology, tumorigenesis, and metabolic profiles. The cell lines may be used to test and identify therapeutic targets and to screen for molecular therapeutic agents. | 09-26-2013 |
20130280803 | DEFINED CELL CULTURING SURFACES AND METHODS OF USE - In one aspect, there is provided a cell culturing substrate including: | 10-24-2013 |
20130280804 | DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELLS TO MULTIPOTENT NEURAL CREST CELLS - The present invention relates to the differentiation of human pluripotent cells, including human pluripotent stems cells to produce a self-renewing multipotent neural crest cell population in a single step method without the requirement of isolation of intermediate cells and without appreciable contamination (in certain preferred instances, virtually none) with Pax6+ neural progenitor cells in the population of p75+ Hnk1+ Ap2+ multipotent neural crest-like cells. The multipotent neural crest cell population obtained can be clonally amplified and maintained for >25 passages (>100 days) while retaining the capacity to differentiate into peripheral neurons, smooth muscle cells and mesenchymal precursor cells. | 10-24-2013 |
20130288366 | Synthetic culture platform and methods of using - The present invention provides a synthetic cell culture platform, comprising: a two dimensional and/or three dimensional surface comprising peptides conjugated to said surface and methods of using the platform for increasing cell adhesion, stable attachment and/or proliferation of cells grown on the platform and for promoting differentiation of neural stem cells into neurons. | 10-31-2013 |
20130302889 | Differentiation of Bone Marrow Cells into Neuronal Cells and Uses Therefor - The present invention relates to methods of inducing differentiation of mammalian bone marrow stromal cells into neuronal cells by contacting marrow stromal cells with a neuronal differentiation-inducing compounds. Neuronal differentiation-inducing compounds of the invention include anti-oxidants such as, but not limited to, beta-mercaptoethanol, dimethylsulfoxide, butylated hydroxyanisole, butylated hydroxytoluene, ascorbic acid, dimethylfumarate, and n-acetylcysteine. Once induced to differentiate into neuronal cells, the cells can be used for cell therapy, gene therapy, or both, for treatment of diseases, disorders, or conditions of the central nervous system. | 11-14-2013 |
20130344598 | NEURAL CELL PURIFICATION FOR TRANSPLANTATION - Aspects of implementations wherein reductions in contamination in transplantation cell population are disclosed herein, including incubating a suspended population of live cells in a culture medium with a substrate having surface that promotes cell to cell adhesion. During the process, the incubating is in absence of substantial mechanical disturbance of the culture medium and cells are in the culture medium for a predetermined period of time. A resulting population of cells which is relatively reduced or eliminated in contaminants is taught. Said population of cells may be collectable by centrifugation and is separable from said contaminants by centrifugation. | 12-26-2013 |
20140080209 | METHOD OF INHIBITING APOLIPOPROTEIN-E EXPRESSION COMPRISING ADMINISTERING A TRIARYLMETHYL AMINE COMPOUND - This invention offers an effective method of inhibiting the expression of apolipoprotein E by mammalian cells. Apolipoprotein E is a protein that plays a significant role in the development of Alzheimer's Disease in humans. The method comprises administering an effective amount of a triarylmethyl amine compound having the general formula: | 03-20-2014 |
20140093957 | Cells Useful for Immuno-Based Botulinum Toxin Serotype A Activity Assays - The present specification discloses clonal cell lines susceptible to BoNT/A intoxication, methods of producing such clonal cell lines, and methods of detecting Botulinum toxin serotype A activity using such clonal cell lines. | 04-03-2014 |
20140134729 | Synthetic Surfaces for Culturing Stem Cell Derived Oligodendrocyte Progenitor Cells - Synthetic surfaces suitable for culturing stem cell derived oligodendrocyte progenitor cells contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived oligodendrocyte progenitor cells in chemically defined media. | 05-15-2014 |
20140295547 | METHOD TO CONSTRUCT IN-VITRO HUMAN BLOOD BRAIN BARRIER MODEL - A method to construct an in-vitro human blood brain barrier (BBB) model is disclosed, which comprises steps: attaching suspension liquids of human brain vascular pericytes (HBVPs) and human astrocytes (HAs) by a ratio of 1:1, 1:2, or 1:6 to a bottom surface of a filter membrane of a culture dish to plant HBVPs and HAs on the bottom surface; filling a suspension liquid of human brain microvascular endothelial cells (HBMECs) into a top surface of the filter membrane to plant HBMECs on the top surface; placing the culture dish in a well plate containing a culture medium, and placing the well plate in a carbon-dioxide incubator; replacing the culture medium with a condition medium; and replacing the condition medium once daily for a plurality of days. Thereby is constructed an in-vitro human BBB model of high medical research availability. | 10-02-2014 |
20140356951 | DIFFERENTIATING INDUCED PLURIPOTENT STEM CELLS INTO GLUCOSE-RESPONSIVE, INSULIN-SECRETING PROGENY - This document provides methods and materials related to differentiating iPS cells into glucose-responsive, insulin-secreting progeny. For example, methods and material for using indolactam V (ILV) and glucagon like peptide-1 (GLP-1) to produce glucose-responsive, insulin-secreting progeny from iPS cells are provided. | 12-04-2014 |
20150031129 | METHOD FOR PROLIFERATING STEM CELLS BY ACTIVATING C-MET/HGF SIGNALING AND NOTCH SIGNALING - The present invention relates to stem cells in which a gene that activates signaling is introduced and to a method for proliferating the stem cells. More specifically, the invention relates to a method of significantly increasing the ability of stem cells to proliferate, either by transfecting stem cells with the Notch intracellular domain (NICD) to activate the Notch signaling pathway, or by transfecting stem cells with the c-MET gene and treating the transfected stem cells with the HGF ligand protein to activate the c-MET/HGF signaling pathway. According to the present invention, as a result of activating the Notch signaling pathway or the c-MET/HGF signaling pathway, stem cells having an excellent ability to proliferate can be produced in large amounts. Particularly, since neural stem cells which have been difficult to culture in vitro can be proliferated in large amounts, thus the neural stem cells will be more useful for the preparation of cell therapeutic agents for treating cranial nerve diseases. | 01-29-2015 |
20150037885 | METHOD OF PRODUCING HUMAN RETINAL PIGMENTED EPITHELIAL CELLS - The production of human retinal pigment epithelial cells having a step of incubating human pluripotent stem cells with binder molecules binding to terminal N-acetyllactosamine (Galβ1-4Glc NAc) and/or blood group H determinant type 2 (Fucα1-2Galβ1-4Glc NAc). A method of using lectin ECA in a culture of human pluripotent stem cells in order to support the stem cells to differentiate into retinal pigment epithelial cells is also disclosed. | 02-05-2015 |
20150087058 | STEM CELL CULTURE MEDIUM AND METHOD FOR CULTURING STEM CELLS USING SAME - The present invention relates to a stem cell culture medium which can be substituted for a conventional stem cell culture medium containing the heterologous protein fetal bovine serum, and more particularly to a stem cell culture medium containing a basal medium and a knockout serum replacement and a method of culturing stem cells using the same. According to the invention, a high purity of stem cells having a reduced ability to spontaneously differentiate can be obtained without having to use the heterologous protein fetal bovine serum and expensive growth factors (EGF and bFGF), and thus the efficacy of stem cell therapy can be significantly increased. | 03-26-2015 |
20150118749 | RETINAL PIGMENT EPITHELIAL CELLS DIFFERENTIATED FROM EMBRYONIC STEM CELLS WITH NICOTINAMIDE AND ACTIVIN A - The present invention concerns RPE cells obtainable by directed differentiation from stem cell, particularly, human stem cells. It has been specifically found that culturing stem cells in the presence of one or more member of the TGFβ superfamily, such as Activin A) induced directed differentiation into mature and functional RPE cells. This was evidenced by the expression of markers specific to mature RPE cells, including MiTF-A, RPE65 or Bestrophin). In accordance with one particular embodiment, the cells are a priori cultured with nicotinamide (NA) which was found to augment the cells' response to the inductive effect of the one or more member of the TGFβ superfamily. The invention also provides methods of performing the directed differentiation, as well as methods for use of the resulting RPE cells. | 04-30-2015 |
20150368614 | PERIVASCULAR MESENCHYMAL PRECURSOR CELLS - Mesenchymal precursors cells have been isolated from perivascular niches from a range of tissues utilizing a perivascular marker. A new mesenchymal precursor cell phenotype is described characterized by the presence of the perivascular marker 3G5, and preferably also alpha smooth muscle actin together with early developmental markers such as MUC 18, VCAM-1 and STRO-1 | 12-24-2015 |
20160068806 | COMPOSITIONS AND METHODS FOR PRECISE PATTERNING OF POSTERIOR NEUROECTODERM FROM HUMAN PLURIPOTENT STEM CELLS - Described herein are methods, compositions, and kits for directed differentiation of human pluripotent stem cells into caudal lateral epiblasts, posterior neuroectoderm or posterior neuroepithelium, or motor neurons having specified HOX gene expression pattern mirroring a desired position along the rostral-caudal axis during hindbrain and spinal cord development. Also described are isolated populations of cells including caudal lateral epiblasts, posterior neuroectoderm, posterior neuroepithelium, or motor neurons having a HOX gene expression pattern specified to correspond to the HOX gene expression pattern associated with a desired rostral-caudal axis position. | 03-10-2016 |
20160152950 | Methods of Maintaining, Expanding and Differentiating Neuronal Subtype Specific Progenitors | 06-02-2016 |
20160251618 | SUBSTANTIALLY PURE HUMAN RETINAL PROGENITOR, FOREBRAIN PROGENITOR, AND RETINAL PIGMENT EPITHELIUM CELL CULTURES AND METHODS OF MAKING THE SAME | 09-01-2016 |