Class / Patent application number | Description | Number of patent applications / Date published |
435354000 | Mouse (i.e., Mus) | 79 |
20080213885 | COMPOSITION AND METHOD FOR ENABLING PROLIFERATION OF PLURIPOTENT STEM CELLS - The present disclosure is directed to the development of compositions, such as extracellular matrices, and processes for using the same, for culturing stem cells in vitro in an undifferentiated state. In this regard, it has been discovered that when pluripotent mouse and human embryonic stem cells are cultured on plates coated with recombinant laminin-10 (laminin-511) or laminin-5 (laminin-322), or their functional domains, the embryonic stem cells proliferated and maintained their pluripotency. | 09-04-2008 |
20080268533 | Methods and Compositions Utilizing Myc and Gsk3Beta to Manipulate the Pluripotency of Embryonic Stem Cells - The present invention provides methods for stabilizing pluripotent cells through the transcriptional activation of c-myc. Alternatively, the cells are stabilized through the transcriptional activation of c-myc, and the stabilization of c-myc protein levels. c-myc protein can be stabilized through the inhibition of GSK3β or through other components of the cellular machinery that impact on c-myc stability. The invention contemplates the stabilized pluripotent cells produced using the methods described herein. Methods for the identification of compounds that modulate the stabilization of pluripotent cells through modulating transcriptional activation of c-myc, stabilization of c-myc protein levels, and/or inhibition of GSK3β activity are also contemplated. | 10-30-2008 |
20090011500 | G+C RICH BINDING PROTEIN - A novel transcription regulator of G+C-rich promoters, the murine G+C-rich Promoter Binding Protein (mGPBP), and its interaction pattern with other transcription factors are disclosed. A human homologue of mGPBP, hGPBP, is described as well. | 01-08-2009 |
20090011501 | NOVEL DEATH DOMAIN PROTEINS - In accordance with the present invention, there are provided novel Death Domain (DD), Death Effector Domain (DED) and NB-ARC domain proteins. The invention also provides nucleic acid molecules encoding DD, DED and NB-ARC domain proteins, vectors containing these nucleic acid molecules and host cells containing the vectors. The invention also provides antibodies that can specifically bind to invention DDs, DEDs or NB-ARC domains. Such DDs, DEDs and NB-ARC domains and/or anti-DD, anti-DED or anti-NB-ARC domain antibodies are useful for discovery of drugs that suppress infection, autoimmunity, inflammation, allergy, allograft rejection, sepsis, and other diseases. | 01-08-2009 |
20090081781 | Synthetic and standardized prion infectuous material, and uses thereof as an injecting inoculum - The invention concerns a novel, synthetic, standardised, soluble, reproducible and easy-to-handle infectious material of the prion type, consisting of a cell lysate or culture supernatant from stable transgenic cells expressing a prion protein PrP and supporting replication of the pathogenic form, PrPsc, of the said Prp. | 03-26-2009 |
20090087906 | NOVEL CELL-BASED ASSAYS FOR G-PROTEIN-COUPLED RECEPTOR-MEDIATED ACTIVITIES - Disclosed are compositions and methods for their use, such as in identifying G-protein-coupled receptors, ligands and compounds that modulate the activities of G-protein-coupled receptors. The compositions and methods employ cyclic nucleotide-gated channels and fluorescence dyes in detecting changes of intracellular cAMP levels in response to the stimulation of G-protein-coupled receptors. Activation of the G-protein-coupled receptors can be detected in a variety of assays, including cell-based imaging assays with fluorescence microscopes and high throughput assays with multi-well plates and fluorescence plate readers. | 04-02-2009 |
20090111176 | Trehalose transporter gene and method of introducing trehalose into cells - There are provided trehalose transporter gene and a method of introducing trehalose into cells by using the gene. Candidates for the trehalose transporter genes were searched in | 04-30-2009 |
20090317906 | GENE SILENCING USING SENSE DNA AND ANTISENSE RNA HYBRID CONSTRUCTS COUPLED TO PEPTIDES FACILITATING THE UPTAKE INTO CELLS - The present invention generally relates to gene silencing using sense DNA (sDNA)-antisense RNA (aRNA) hybrids wherein the sense DNA strand is coupled to a peptide which facilitates the uptake of the hybrid into cells. | 12-24-2009 |
20100015705 | Generation of Clonal Mesenchymal Progenitors and Mesenchymal Stem Cell Lines Under Serum-Free Conditions - Methods for obtaining multipotent mesenchymal stem cells under serum-free conditions and methods for identifying multipotent mesenchymal progenitor cells are disclosed. | 01-21-2010 |
20100041137 | CULTURE MEDIUM CONTAINING KINASE INHIBITORS, AND USES THEREOF - Pluripotent cells are maintained in a self-renewing state in serum-free culture medium comprising a MEK inhibitor, a GSK3 inhibitor and, optionally, an antagonist of an FGF receptor. Pluripotent cells are also maintained in a self-renewing state in serum-free culture medium comprising a MEK inhibitor and an antagonist of an FGF receptor. | 02-18-2010 |
20100144031 | METHODS FOR REPROGRAMMING SOMATIC CELLS - The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state. | 06-10-2010 |
20100173409 | CULTURE MEDIA FOR DEVELOPMENTAL CELLS CONTAINING ELEVATED CONCENTRATIONS OF LIPOIC ACID - A composition and method for in vitro fertilization is provided which uses culture media comprising elevated concentrations of lipoic acid. More specifically, the invention provides culture media for developmental cells having a lipoic acid concentration of 5 μM to 40 μM. Culture media that include lipoic acid at concentrations within the identified range are able to provide blastocysts with increased survival, increased cell numbers, increased inner cell masses and/or increased percentage of the total mass made up by the inner cell compared to blastocysts cultured in a control medium. | 07-08-2010 |
20100197011 | METHOD OF OBTAINING CELL LINES IN A PROTEIN-FREE MEDIUM AND CELL LINES THUS OBTAINED - A method of recovering mammalian cell clones adapted to serum and protein-free media is disclosed. The procedure includes a two-stage adaptation process to grow in that condition. A critical protein concentration interval is disclosed in which cells must grow in order to gain the capacity to survive in serum and protein-free condition, once the cells have grown at the critical interval concentrations, subsequent decreases of the concentration will affect neither viability nor cellular doubling time. The critical protein concentration interval is cell line specific. Furthermore, mammalian cells clones are disclosed, which are stable in serum- and protein-free media for at least 40 generations; additionally, clones disclosed express a recombinant product. The cell clones disclosed produce the humanized anti-EGF-R antibody hR3, the humanized anti-CD6 antibody T1hT, the chimeric anti CD3 antibody T3Q, or fragments thereof. | 08-05-2010 |
20100203632 | METHODS FOR CONTROLLING STEM CELL DIFFERENTIATION - Disclosed herein are methods for controlling stem cell differentiation through the introduction of transgenes having Xic, Tsix, Xite, or Xic flanking region sequences to block differentiation and the removal of the transgenes to allow differentiation. Also disclosed are small RNA molecules and methods for using the small RNA molecules to control stem cell differentiation. Also disclosed are stem cells genetically modified by the introduction of Xic, Tsix, XUe, or Xic flanking region sequences. | 08-12-2010 |
20100221827 | METHODS FOR REPROGRAMMING SOMATIC CELLS - The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state. | 09-02-2010 |
20100233804 | GENERATION OF PLURIPOTENT STEM CELLS USING RECOMBINANT PROTEINS - The present invention provides for methods, compositions, and kits of producing an induced pluripotent stem cell from a mammalian non-pluripotent cell using exogenous transcription factors. | 09-16-2010 |
20100261274 | GENERATION OF CLONAL MESENCHYMAL PROGENITORS AND MESENCHYMAL STEM CELL LINES UNDER SERUM-FREE CONDITIONS - Methods for obtaining multipotent Apelin receptor-positive lateral plate mesoderm cells, mesenchymal stem cells, and mesangioblasts under serum-free conditions are disclosed. | 10-14-2010 |
20100267132 | IN VITRO PRESERVATION OF LIVING ANIMAL CELLS AND COMPOUNDS SUITABLE FOR USE IN THE PRESERVATION OF LIVING ANIMAL CELLS - The invention is directed to compounds of Formula (I) | 10-21-2010 |
20100285580 | Cardiac Conduction System (CCS) Progenitor Cells and Uses Thereof - The present invention provides isolated stem cells and progenitor cells, including HCN4-expressing CCS progenitor cells, HCN4- and Islet 1-expressing sinoatrial node cells or progenitor cells thereof, and Islet 1-expressing cardiovascular stem cells that do not express HCN4. The invention further provides differentiated cell types derived from the CCS progenitor cells and cardiovascular stem cells of the invention. In addition, the invention provides methods of differentiating the cells of the invention, methods for screening for agents that affect the differentiation of the cells of the invention, methods for evaluating the potential toxicity of drugs using the cells of the invention, cell therapy using the cells of the invention, and methods of treating heart arrhythmia by ablating HCN4-expressing cells. | 11-11-2010 |
20100304478 | METHOD FOR ISOLATING RENAL STEM/PROGENITOR CELLS, RENAL STEM/PROGENITOR CELLS AND THERAPEUTIC AGENT FOR RENAL DISEASE - It is intended to provide a method for noninvasively isolating human renal stem/progenitor cells, an isolated renal stem/progenitor cells, a therapeutic agent for renal disease, mouse mesenchymal cells which can be used for isolating human renal stem/progenitor cells and a culture supernatant of the same. Renal stem/progenitor cells are isolated by primarily culturing cells contained in the urine of a patient having renal disease in a medium containing mouse mesenchymal cells identified by the deposition number of FERM ABP-10865 or a culture supernatant of the same, staining the obtained primarily cultured cells with Hoechst 33342 and separating a weak-positive or negative fraction. | 12-02-2010 |
20110014692 | METHOD OF DERIVING PROGENITOR CELL LINE - We disclose a method comprising: (a) providing an embryonic stem (ES) cell; and (b) establishing a progenitor cell line from the embryonic stem cell; in which the progenitor cell line is selected based on its ability to self-renew. Preferably, the method selects against somatic cells based on their inability to self-renew. Preferably, the progenitor cell line is derived or established in the absence of co-culture, preferably in the absence of feeder cells, which preferably selects against embryonic stem cells. Optionally, the method comprises (d) deriving a differentiated cell from the progenitor cell line. | 01-20-2011 |
20120058554 | Synthetic Polysaccharide Microcarriers for Culturing Cells - A cell culture polysaccharide microcarrier includes (1) a cross-linked polysaccharide microcarrier base having a neutral or negative charge at pH 7, and (ii) a polypeptide conjugated to the base. The polypeptide may contain a cell adhesive sequence, such as RGD. Cells cultured with such microcarriers exhibit peptide-specific binding to the microcarriers. | 03-08-2012 |
20120058555 | RNAi EXPRESSION CONSTRUCTS - The present invention provides compositions and methods suitable for expressing 1-x RNAi agents against a gene or genes in cells, tissues or organs of interest in vitro and in vivo so as to treat diseases or disorders. | 03-08-2012 |
20120064623 | Process for the Culturing of Cells - The invention relates to a process for the culturing of cells, preferably El-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture. | 03-15-2012 |
20120083034 | EX HOST MATURATION OF GERMLINE STEM CELLS - Methods are provided for the ex host maturation of immature germline cells into haploid gametes for restoration of fertility in patients in need thereof. | 04-05-2012 |
20120142093 | METHOD FOR INDUCING DIFFERENTIATION OF PLURIPOTENT STEM CELLS INTO NEURAL PRECURSOR CELLS - The present invention provides a method for inducing differentiation of pluripotent stem cells into neural precursor cells, comprising culturing the pluripotent stem cells in the presence of a small molecule BMP inhibitor, and induced neural precursor cells prepared by this method. | 06-07-2012 |
20120149107 | PROCESS FOR THE CULTURING OF CELLS - The invention relates to a process for the culturing of cells, preferably E1-immortalized HER cells, more preferably PER.C6 cells in a reactor in suspension in a cell culture medium, wherein the cells produce a biological substance, preferably an antibody, wherein at least one cell culture medium component is fed to the cell culture and wherein the cell culture comprising the cells, the biological substance and cell culture medium is circulated over a separation system and wherein the separation system separates the biological substance from substances having a lower molecular weight than the biological substance and wherein the biological substance is retained in or fed back into the reactor. Preferably part of the substances of lower molecular weight is continuously removed from the cell culture. | 06-14-2012 |
20120178158 | ADULT ANIMALS GENERATED FROM INDUCED PLURIPOTENT CELLS - The present invention provides methods and compositions for generating and using induced pluripotent stem cells. | 07-12-2012 |
20120184030 | METHODS FOR ESTABLISHING SYMBIOSES - The present invention relates to methods for establishing a symbiosis, including the following steps: selecting an organism or an organelle to constitute the symbiont and an organism to constitute the host, the latter not existing naturally in a symbiotic relationship; contacting the symbiont and the host; and maintaining the combination of the symbiont and the host. | 07-19-2012 |
20120202286 | rbLIF PROTEIN FOR USE IN EMBRYONIC STEM CELL CULTURES - Provided herein methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell on a rabbit feeder cell layer, wherein the rabbit feeder layer expresses a leukemia inhibitory factor (LIF) protein. Also provided are methods of maintaining an embryonic stem cell culture in an undifferentiated state, wherein the method includes culturing an embryonic stem cell in a culture medium comprising rabbit leukemia inhibitory factor (rbLIF) protein. Also provided are polynucleotide and amino acid sequences of a rabbit leukemia inhibitory factor protein (rbLIF). | 08-09-2012 |
20120270313 | Compositions and Methods for Establishing and Maintaining Stem Cells in an Undiffferentiated State - The present invention embraces compositions and methods for establishing and maintaining stem cells and inhibiting stem cell differentiation using a selective Protein Kinase C (PKC) inhibitor. | 10-25-2012 |
20130011921 | METHOD FOR PRODUCTION OF ARTIFICIAL PLURIPOTENT STEM CELL - Disclosed is a method for producing an iPS cell having a very similar gene expression pattern to that of an ES cell with high efficiency. Specifically disclosed is a method for producing an artificial pluripotent stem cell (an iPS cell), which comprises the steps of: introducing a pluripotency-inducing factor comprising at least an Myc family gene or an Myc family protein into a somatic cell; and culturing the somatic cell in the presence of a sirtuin inhibitor and/or a poly-ADP ribose polymerase (PARP) inhibitor. | 01-10-2013 |
20130059374 | Novel Therapeutical Tools and Methods for Treating Blindness - The present inventions relates to the use of an isolated nucleic acid molecule comprising a nucleotide sequence coding for a hyperpolarizing light-gated ion channel or pump gene from an archeon or for a light-active fragment of said gene, or the nucleotide sequence complementary to said nucleotide sequence, for treating or ameliorating blindness. The light-gated ion channel or pump gene can be a halorhodopsin gene. | 03-07-2013 |
20130059375 | METHOD FOR PREPARING ES CELLS - The present invention provides a method of producing a mammalian ES cell, including cultivating a mammalian inner cell mass in a medium containing adrenocorticotropic hormone, an FGF receptor inhibitor, an MEK activation inhibitor and a GSK3 inhibitor, and isolating a mammalian ES cell from the culture. | 03-07-2013 |
20130095565 | miRNA-Regulated Differentiation-Dependent Self-Deleting Cassette - Targeting constructs and methods of using them are provided for differentiation-dependent modification of nucleic acid sequences in cells and in non-human animals. Targeting constructs comprising a promoter operably linked to a recombinase are provided, wherein the promoter drives transcription of the recombinase in an differentiated cell but not an undifferentiated cell. Promoters include Blimp1, Prm1, Gata6, Gata4, Igf2, Lhx2, Lhx5, and Pax3. Targeting constructs with a cassette flanked on both sides by recombinase sites can be removed using a recombinase gene operably linked to a 3′-UTR that comprises a recognition site for an miRNA that is transcribed in undifferentiated cells but not in differentiated cells. The constructs may be included in targeting vectors, and can be used to automatically modify or excise a selection cassette from an ES cell, a non-human embryo, or a non-human animal. | 04-18-2013 |
20130102074 | METHODS FOR REPROGRAMMING SOMATIC CELLS - The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state. | 04-25-2013 |
20130122586 | METHOD OF CULTURING PANCREATIC ISLET-LIKE TISSUES BY A TISSUE COMPLEX OF PANCREAS-DERIVED NON-ENDOCRINAL EPITHELIAL CELLS AND VASCULAR ENDOTHELIAL CELLS - According to embodiments, a method of producing insulin-producing tissues (IPTs) by culturing comprises: preparing non-endocrinal epithelial cells (NEECs) and vascular endothelial cells (VECs), which have been isolated or originated from postnatal pancreata, preferably by capturing of NEECs by collagen; culturing in vitro the NEECs and the VECs at least partly separately from each other; and then generating in vitro a tissue complex (IPTs) that contains both the NEECs and the VECs. In another embodiment, the native islet cells are seeded on a monolayer of VECs that have preferably been separately cultured and purified. In a further embodiment, a method of enriching NEECs comprises: culturing NEECs adhering to a container or substrate; removing NEECs by treating with a tissue-dissociation enzyme to leave left-over cells (LOCs) still attached on the container or substrate; and culturing NEECs in a medium conditioned by, or in the presence of the LOCs. | 05-16-2013 |
20130157357 | LENTIVIRAL VECTORS FOR THE PREPARATION OF IMMUNOTHERAPEUTICAL COMPOSITIONS - The invention relates to an immunogenic composition comprising a recombinant vector characterized in that it comprises a polynucleotide comprising the cis-acting central initiation region (cPPT) and the cis-acting termination region (CTS), these regions being of retroviral or retroviral-like origin, said vector comprising in addition a defined nucleotide sequence (transgene or sequence of interest) and regulatory signals of retrotranscription, expression and encapsidation of retroviral or retroviral-like origin, wherein the composition is capable of inducing or of stimulating a cell-mediated response for instance a CTL (Cytotoxic T Lymphocytes) response or a CD4 response, against one or several epitopes encoded by the transgene sequence present in the vector. | 06-20-2013 |
20130189775 | MODULATION OF THE MAMMALIAN RECEPTOR mTOR TO INHIBIT STEM CELL DIFFERENTIATION INTO NEURONS - The present application relates to compositions and methods for inhibiting the differentiation of isolated embryonic stem cells, induced pluripotent stem cells, parthenogentic stem cells, or isolated embryoid bodies into neuronal progenitor cells or neuron cells comprising a mTOR inhibitor and an effective amount of cell culture growth media. The mTOR inhibitor can be Rapamycin. | 07-25-2013 |
20130236957 | EXPRESSION VECTOR AND METHODS OF PRODUCING HIGH LEVELS OF PROTEINS - A process for high expression of protein of interest using an expression vector. The process comprises at least the following regulatory elements:
| 09-12-2013 |
20130236958 | Viral expression plasmids for production of proteins, antibodies, enzymes, virus-like particles and for use in cell-based assays - This disclosure shows that the EBV FR-element comprised of EBNA1 multimeric binding sites can provide the stable maintenance replication function to the mouse polyomavirus (PyV) core origin plasmids in the presence of BPV-1 E2 protein and PyV large T-antigen (LT). | 09-12-2013 |
20130260456 | RECOMBINANT PROTEINS HAVING HAEMOSTATIC ACTIVITY AND CAPABLE OF INDUCING PLATELET AGGREGATION - Recombinant proteins capable of inducing platelet aggregation and uses thereof. | 10-03-2013 |
20130273650 | VECTORS AND METHODS FOR RECOMBINANT PROTEIN EXPRESSION - The present invention discloses a series of eukaryotic expression vectors utilizing the reduction of transcription read-through events to create stable and high-yield cell lines for recombinant protein expression. The vectors comprise more than one polyadenylation signal or one or more polyadenylation signals plus other DNA fragment which is known to enhance transcription termination to control the expression level of selection marker, with the configuration to transcribe the minimal level of full-length bicistronic mRNA to express the selection marker, which can be used to create stable cell lines at high expression levels, without the need for drug selection or drug mediated gene amplification. | 10-17-2013 |
20130288365 | Cell Line, System and Method for Optical-Based Screening of Ion-Channel Modulators - A variety of applications, systems, methods and constructs are implemented for use in connection with screening of ion-channel modulators. Consistent with one such system, drug candidates are screened to identify their effects on cell membrane ion channels and pumps. The system includes screening cells having light responsive membrane ion switches, voltage-gated ion switches and fluorescence producing voltage sensors. A chemical delivery device introduces the drug candidates to be screened. An optical delivery device activates the light responsive ion switches. An optical sensor monitors fluorescence produced by the voltage sensors. A processor processes data received from the optical sensor. A memory stores the data received from the optical sensor. | 10-31-2013 |
20140099710 | COMPOSITIONS FOR THE IN VITRO DERIVATION AND CULTURE OF EMBRYONIC STEM (ES) CELL LINES WITH GERMLINE TRANSMISSION CAPABILITY AND FOR THE CULTURE OF ADULT STEM CELLS - The present invention is directed to a method of deriving pluripotent embryonic stem cells from mouse blastocysts or from primordial germ cells from a post-implantation mouse embryo, or of maintaining or growing pluripotent embryonic stem cells from a mouse, or of expanding human hematopoietic stem cells or human hematopoietic precursor cells. The methods include the step of cultivating the stem cells or precursor cells for at least one passage in a culture medium preconditioned by the rabbit fibroblast cell line Rab9 (ATCC catalogue CRL1414) and containing less than 0.1 ng/ml Leukemia Inhibitory Factor (LIF). | 04-10-2014 |
20140099711 | METHODS AND SYSTEMS FOR OPTIMIZING PERFUSION CELL CULTURE SYSTEM - Methods and perfusion culture systems are disclosed. The systems and methods relate to decreasing the starting perfusion rate, resulting in increased residence time of the cells in the bioreactor and the cell retention device, and/or concomitantly increasing the starting bioreactor volume or decreasing the starting cell retention device volume, or both. Other method embodiments include increasing the concentrations of individual components of the tissue culture fluid, and adding a stabilizer of the degradation of the recombinant protein. | 04-10-2014 |
20140256036 | HIGH-DENSITY SPOT SEEDING FOR TISSUE MODEL FORMATION - A model of tissue is produced by steps comprising seeding cells at a selected concentration on a support to form a cell spot, incubating the cells to allow the cells to partially attach, rinsing the cells to remove any cells that have not partially attached, adding culture medium to enable the cells to proliferate at a periphery of the cell spot and to differentiate toward a center of the cell spot, and further incubating the cells to form the tissue. The cells may be C2C12 cells or other subclones of the C2 cell line, H9c2(2-1) cells, L6 cells, L8 cells, QM7 cells, Sol8 cells, G-7 cells, G-8 cells, other myoblast cells, cells from other tissues, or stem cells. The selected concentration is in a range from about 1×10 | 09-11-2014 |
20140273207 | LABEL-FREE IDENTIFICATION OF STEM CELL-DIFFERENTIATED CELLS - Methods are disclosed for isolation of a stem-cell derived differentiated cell, which method entails illuminating an incident light onto a plurality of stem-cell derived cells that comprise at least an undifferentiated cell and a differentiated cell possessing a noncentrosymmetric structure, wherein the differentiated cell generates second-harmonic light from the incident light; and isolating the differentiated cell identified by the second-harmonic light. Devices for carrying out the methods are also provided. | 09-18-2014 |
20140363886 | METHOD FOR PREPARING INDUCED PARAXIAL MESODERM PROGENITOR (IPAM) CELLS AND THEIR USE - The present invention relates to an ex vivo method for preparing induced paraxial mesoderm progenitor (iPAM) cells, said method comprising the step of culturing pluripotent cells in an appropriate culture medium comprising an effective amount of an activator of the Wnt signaling pathway and an effective amount of an inhibitor of the Bone Morphogenetic Protein (BMP) signaling pathway. | 12-11-2014 |
20140363887 | DLL3 MODULATORS AND METHODS OF USE - Novel modulators, including antibodies and derivatives thereof, and methods of using such modulators to treat proliferative disorders are provided. | 12-11-2014 |
20140370596 | Neural Stem Cells - A homogenous, symmetrically dividing population of adherent neural stem cells is obtained from ES cells or foetal or adult brain isolates, using an activator of a signalling pathway downstream of a receptor of the EGF receptor family, optionally in combination with an activator of a signalling pathway downstream of an FGF receptor. The neural stem cell population is highly pure and retains the ability to differentiate into neurons after in excess of 100 passages. | 12-18-2014 |
20150037883 | METHOD FOR DERIVATION AND LONG-TERM ESTABLISHMENT OF GROUND STATE PLURIPOTENT EMBRYONIC STEM CELLS - The various embodiments herein provide a method for derivation and long term establishment of ground state pluripotent embryonic stem cells. Further the embodiments herein provides a method to inhibit the ERK and TGF β signalling pathways for long term maintenance of the embryonic stem cells. The R2i mouse embryonic stem (ES) cells are derived from 3.5 day blastocysts. The mouse ES cells are cultured in media containing R2i and 2i inhibitors of ERK and TGF β pathways. The ES cells are subjected to in vitro and in vivo differentiation. The ES cells are subjected to RT-PCR and qRT-PCR, flow cytometry and karyotyping. The result reveals that the R2i maintains the ground state of ES cells and self renewal. Also R2i increases embryonic cleavage and clonal propagation of ES. Further R2i asserts genomic integrity and pluripotency of ES. | 02-05-2015 |
20150044768 | METHODS AND COMPOSITIONS FOR PRODUCTION OF RECOMBINANT PROTEIN IN HBX-EXPRESSING MAMMALIAN CELLS - The method of the invention provides for producing a heterologous protein in mammalian host cells having nucleic acid encoding Hepatitis B X protein and the heterologous protein, by growing mammalian host cells selected from the group consistng of HKB11, CHO, BHK21, C2C12, and HEK293 cells, by growing mammalian host cells in non-adherent suspension culture, or by growing mammalian host cells which contain nucleic acid providing exogenous X-box Binding Protein, XBP1s. The conditions should be such that HBx, exogenous XBP1s if present, and the heterologous protein are expressed by the mammalian cells. The invention includes compositions for carrying out the method. | 02-12-2015 |
20150118747 | ELECTROSTRETCHED POLYMER MICROFIBERS FOR MICROVASCULATURE DEVELOPMENT - An in vitro model system that guides the development of microvasculature, recapitulating the detailed organization of both its cellular and a-cellular components is established. Use of electrostretched fibrin microfibers enables both endothelial layer organization and co-culture of supporting perivascular (mural) cells such as vascular smooth muscle cells and pericytes. The fiber curvature affects the circumferential deposition of endothelial-produced ECM independently of cellular organization and induces deposition of higher quantities of vascular ECM proteins. Further, a luminal multicellular microvascular structure is disclosed. | 04-30-2015 |
20150368617 | METHOD FOR FORMING ENDOTHELIAL CELLS - The present application discloses a method of promoting differentiation and expansion of endothelial cells (EC) and cardiomyocytes from a pluripotent cell, which includes contacting the pluripotent cell with a Rho-associated protein kinase (ROCK) suppressor. | 12-24-2015 |
20160017288 | METHOD FOR INDUCING THE FORMATION OF ISLET STRUCTURES AND IMPROVING BETA CELL FUNCTION - Insulin producing β cells are found in three dimensional (3D) structures, the Islet of Langerhans. The 3D structure is required for normal β cell function and survival. β cell pseudoislets (PIs) are useful for study of β cell physiology. Co-culturing of primary human islets and β cell lines together with islet-derived epithelial cells can improve β cell function and survival and maintain the cells' 3D structure, resulting a rapid and spontaneous formation of free-floating PIs. β cells in PIs were similar in size to native islets and showed increased percentage of pro-insulin-positive cells, increased insulin gene expression in response to glucose stimulation, improved glucose-stimulated insulin secretion, and reduced β cell death. Key ECM proteins, absent in monolayer β cells, are deposited by iECs in and round the PIs. iEC induced PIs are a useful tool for examining β-cell/iEC interactions and studying β-cell function in a native 3D configuration. | 01-21-2016 |
20160032248 | COMPOSITIONS AND METHODS FOR OBTAINING ENRICHED MESENCHYMAL STEM CELL CULTURES - The disclosure provides a method of culturing cells of the mesenchymal cell lineage, said method comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. The disclosure also provides a method of culturing cells from bone marrow and/or compact bone to enrich the cells with cells of the mesenchymal cell lineage comprising contacting the cells with a culture media comprising a CSF1R kinase inhibitor. Cell culture media comprising a CSF1R kinase inhibitor and useful for culturing cells of the mesenchymal cell lineage and/or enriching cells of the mesenchymal cell lineage is also provided. | 02-04-2016 |
20160060597 | FUNCTIONAL MYELINATION OF NEURONS - Hair follicle bulge region/LLP region CD34(+) MeSCs can be isolated from mammalian skin bearing hair follicles. These cells are multipotent and retain the ability to differentiate into cells of neural crest lineage, including glia-like cells that express the glial marker Gfap, and are able to express myelin basic protein, and to remyelinate naked (unmyelinated or demyelinated) neuronal processes with a functional, dense myelin sheath. These cells of neural crest lineage can be used to produce a dense myelin sheath on neurons which lack myelin due to genetic defect, trauma, toxin, infection, or disease process. Therefore, embodiments of the invention provide methods for preparing such cells, the cells themselves and compositions containing the cells, as well as methods for using the cells. | 03-03-2016 |
20160115447 | COMPOSITIONS AND METHODS FOR IMPROVING INDUCED NEURON GENERATION - The present inventions relate to methods and compositions useful for improving the efficiency of inducing the generation of neurons from non-neuronal cell types, for example, by contacting the cell or cell culture medium with one or more agents which inhibit Activin and/or PLK1 signaling. Also disclosed are methods for promoting neuron survival, for example, by inhibiting Activin and/or PLK1 signaling, and methods for promoting the survival of intermediates in a cell differentiation pathway, for example, by inhibiting Activin and/or PLK1 signaling. | 04-28-2016 |
20160115456 | METHODS FOR REPROGRAMMING SOMATIC CELLS - The invention provides methods for reprogramming somatic cells to generate multipotent or pluripotent cells. Such methods are useful for a variety of purposes, including treating or preventing a medical condition in an individual. The invention further provides methods for identifying an agent that reprograms somatic cells to a less differentiated state. | 04-28-2016 |
20160194604 | Compositions And Methods For Epithelial Stem Cell Expansion And Culture | 07-07-2016 |
20220135943 | CELL CULTURE MEDIUM AND METHOD FOR GENERATION OF EPITHELIAL ORGANOIDS FROM EPITHELIAL STEM CELLS - The present invention provides a cell culture for obtaining an epithelial organoid, the cell culture comprising i) epithelial stem cells, or tissue fragments comprising said epithelial stem cells, ii) a basal medium for animal or human cells, iii) a Bone Morphogenetic Protein (BMP) inhibitor, iv) a mitogenic growth factor selected from the group consisting of epidermal growth factor (EGF), transforming growth factor-alpha (TGF-alpha), basic fibroblast growth factor (bFGF), brain-derived neurotrophic factor (BDNF) and keratinocyte growth factor (KGF), v) at least one soluble culture enhancer, wherein said at least one culture enhancer induces correct polarization of the cells in said cell culture within the developing organoid such as a laminin/entactin complex or entactin, and vi) a Wnt agonist if said epithelial stem cells, or tissue fragments comprising said epithelial stem cells are healthy cells, wherein said at least one soluble culture enhancer in said cell culture is a laminin/entactin complex in a concentration between 0.2 mg/ml and 3.4 mg/ml. A cell culture medium, an in-vitro method for obtaining an epithelial organoid, and an epithelial organoid obtained by said method are also disclosed. | 05-05-2022 |
435355000 | Blood or lymphatic origin or derivative | 5 |
20100248361 | PLATELET PRODUCTION METHODS - The present invention provides platelet production methods. The method comprises the steps of providing a cellular material and culturing the cellular material, wherein platelets are produced. The culturing may be performed on 2D or 3D cell support structure or in suspension culture. In some embodiments, all or a part of the 2D or 3D culturing may be performed in a bioreactor. In some embodiments, the method may further comprise a step of isolating a subset of cells from the starting cellular material, wherein the isolated subset of cells is then cultured, wherein platelets are produced. In yet other embodiments, the method comprises the steps of providing a cellular material, isolating a subset of cells, seeding the subset of cells into a 3D scaffold, culturing the subset of cells in a 3D scaffold, seeding the cultured subset of cells into a bioreactor, culturing the subset of cells in a bioreactor, and harvesting the cells from the bioreactor, wherein platelets are produced. | 09-30-2010 |
20110053267 | METHOD FOR PREPARATION OF PLATELET FROM iPS CELL - It is an object of the present invention to provide a method for efficiently preparing blood cells, such as mature megakaryocytes and platelets, from iPS cells in an in vitro culture system. | 03-03-2011 |
20120122207 | Method for Expanding Monocytes - The invention relates to an ex vivo method for expanding monocytes, macrophages or dendritic cells, which method comprises inhibiting the expression or the activity of MafB and c-Maf in monocytes, macrophages or dendritic cells; and expanding the cells in the presence of at least one cytokine or an agonist of cytokine receptor signaling. | 05-17-2012 |
20130109089 | METHODS FOR REPROGRAMMING SOMATIC CELLS | 05-02-2013 |
20130109090 | METHODS FOR REPROGRAMMING SOMATIC CELLS | 05-02-2013 |
435356000 | L cell or derivative (e.g., Ltk(-), etc.) | 2 |
20130203164 | Human Secreted Proteins - The present invention relates to human secreted polypeptides, and isolated nucleic acid molecules encoding said polypeptides, useful for diagnosing and treating diseases, disorders, and/or conditions (such as immune, cardiovascular, cancer, and other proliferative diseases, disorders, and/or conditions) related to said human secreted proteins. Antibodies that bind these polypeptides are also encompassed by the present invention. Also encompassed by the invention are vectors, host cells, and recombinant and synthetic methods for producing said polynucleotides, polypeptides, and/or antibodies. The invention further encompasses screening methods for identifying agonists and antagonists of polynucleotides and polypeptides of the invention. The present invention further encompasses methods and compositions for inhibiting or enhancing the production and function of the polypeptides of the present invention. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating diseases, disorders, and/or conditions related to these novel human secreted proteins. | 08-08-2013 |
20130230918 | NOVEL COLLECTIN - Novel collectin related molecules i.e., a novel collectin gene comprising a nucleotide sequence set out in SEQ ID NO: 1, and a novel collectin comprising an amino acid sequence set out in SEQ ID NO: 2, which are expected to exhibit anti-bacterial, anti-viral activity or the like especially in human body, and methods in which these molecules are used are provided. | 09-05-2013 |
435357000 | Fibroblast, fibroblast-like cell or derivative (e.g., NIH 3T3, etc.) | 10 |
20080293135 | Co-Culture Bioreactor System - Disclosed are multi-chambered cell co-culture systems. The systems can be utilized to encourage the growth and development of isolated cells in a dynamic three-dimensional in vitro environment. The cell chambers ( | 11-27-2008 |
20090029461 | Immortalised Feeder Cells - The invention relates to an immortalized feeder cell line. The immortalized feeder cell line may be derived from an embryonic fibroblast, which may be a mouse embryonic fibroblast. A culture of an immortalized feeder cell line according to the invention in a suitable culture medium is also provided, as is a composition including an immortalized feeder cell line according to the invention in a suitable carrier or diluent and conditioned medium produced from growth of an immortalized feeder cell line according to the invention. The invention further provides a method of culturing a stem cell including use of a cell line or conditioned medium according to the invention and cells so produced. | 01-29-2009 |
20090130754 | Method for culturing human embryonic stem cells - The present invention relates to a method for culturing human embryonic stem cells (hESCs) in a hESC culture medium comprising a porous membrane, feeder cells being attached to a bottom of the porous membrane and a method for recovering human embryonic stem cells using the same. | 05-21-2009 |
20100227398 | Method for Mixing Object into Gelled Assembly - This invention provides a mixing method by which a mixing object can be uniformly mixed into a gelled assembly within a short time period. The method for mixing a mixing object into a gelled assembly comprises freezing the gelled assembly; melting the frozen assembly to obtain a sol; mixing the resultant sol and the mixing object; and reconstituting the gelled assembly from the sol into which the mixing object has been mixed. | 09-09-2010 |
20110070643 | Utilization of Pharmacological Chaperones to Improve Manufacturing and Purification of Biologics - The present invention provides methods for improving the production of recombinant proteins through the use of pharmacological chaperones for the recombinant proteins. As exemplified by the present invention, the binding of a pharmacological chaperone to a recombinant protein expressed by a cell can stabilize the protein and increase export of the protein out of the cell's endoplasmic reticulum, and increase secretion of the protein by the cell. | 03-24-2011 |
20110250684 | METHOD FOR PRODUCING NEURAL STEM CELLS - In order to provide a method for producing neural stem cells easily and quickly by inducing differentiation of somatic cells directly into neurospheres, dedifferentiation factors are introduced into somatic cells, which are then cultured in suspension in the presence of growth factors to produce the neurospheres, thereby allowing the neural stem cells to be produced quickly without establishing iPS cells. | 10-13-2011 |
20120021509 | ISOLATING METHOD FOR UMBILICAL CORD BLOOD-DERIVED PLURIPOTENT STEM CELLS EXPRESSING ZNF281 - The present invention relates to a method for isolating pluripotent/multipotent stem cells derived from umbilical cord blood, characterized by culturing monocytes isolated from umbilical cord blood in a culture vessel containing fibronectin and then harvesting stem cells from the culture, the umbilical cord blood-derived pluripotent/multipotent stem cells isolated thereby; and a cell therapeutic agent containing the pluripotent/multipotent stem cells derived from umbilical cord blood or cells differentiated therefrom. The present invention also relates to a novel culture media for stem cells, a culture method for stem cells which is characterized by culturing and proliferating stem cells in the culture media, and a method for increasing stemness of stem cells which is characterized by a sphere culture or a three-dimensional culture of stem cells. | 01-26-2012 |
20140141509 | Self-Renewing Endodermal Progenitor Lines Generated from Human Pluripotent Stem Cells and Methods of Use Thereof - Self-renewing endodermal progenitor lines generated from human pluripotent stem cells and methods of use thereof are disclosed. | 05-22-2014 |
20140147918 | MICRO AND NANO GLASS BALLS EMBEDDED IN A GEL PRESENTING MICROMETER AND NANOMETER SCALE CURVATURE AND STIFFNESS PATTERNS FOR USE IN CELL AND TISSUE CULTURING AND A METHOD FOR MAKING SAME - A substrate for use in culturing cells or tissues. The substrate comprises a gel, one or more microstructures partially embedded within a surface of the gel, the one or more microstructures presenting two different curvatures or presenting two different stiffness values or presenting a combination of different curvatures and different stiffness values, wherein the microstructures are disposed at defined locations within the surface of the gel, and wherein the cells and tissues are cultured on an exposed surface of the microstructures. | 05-29-2014 |
20160096875 | CODON OPTIMIZED IL-15 AND IL-15R-ALPHA GENES FOR EXPRESSION IN MAMMALIAN CELLS - The present invention provides for nucleic acids improved for the expression of interleukin-15 (IL-15) in mammalian cells. The invention further provides for methods of expressing IL-15 in mammalian cells by transfecting the cell with a nucleic acid sequence encoding an improved IL-15 sequence. | 04-07-2016 |