Class / Patent application number | Description | Number of patent applications / Date published |
435347000 | Two or more cell types, per se, in co-culture | 43 |
20090155898 | METHOD FOR PRODUCING DENDRITIC CELLS - Disclosed are embryonic stem cell-derived dendritic cells, genetically modified immature dendritic cells capable of maturation, as well as methods for the production of such cells. In one embodiment, the cells made be produced by a method comprising the steps of providing a population of embryonic stem cells; culturing the embryonic stem cells in the presence of a cytokine or combination of cytokines which brings about differentiation of the embryonic stem cells into dendritic cells; and recovering the dendritic cells from the culture. In a further embodiment, the cells may be genetically modified. | 06-18-2009 |
20100093081 | METHODS AND COMPOSITIONS FOR PROMOTING SURVIVAL & PROLIFERATION OF ENDOTHELIAL CELLS & STIMULATING ANGIOGENESIS - The present invention relates to adenovirus E4ORF 1 gene and to endothelial cells engineered to express the E40RF 1 gene. The present invention also relates to uses of the E40RF 1 gene, and cells expressing the E40RF1 gene, and to compositions comprising the E4ORF 1 gene, or comprising cells expressing the E4ORF 1 gene. | 04-15-2010 |
20100151568 | DEFINITIVE ENDODERM - Disclosed herein are cell cultures comprising definitive endoderm cells and methods of producing the same. Also disclosed herein are cell populations comprising substantially purified definitive endoderm cells as well as methods for enriching, isolating and purifying definitive endoderm cells from other cell types. | 06-17-2010 |
20100227396 | Embryonic Stem Cell-Like Cells - Disclosed is a method for preparing an embryonic stem cell (ESC)-like cell, which includes the steps of: (a) obtaining a first cell population from a mammalian tissue or body fluid, wherein the first cell population comprises adult stem cells; (b) obtaining a second somatic cell population from a mammalian tissue, wherein the mammalian tissue is different from the mammalian tissue in step (a) and the second cell population is different from the first cell population; (c) coculturing the first cell population and the second cell population in a medium for a period of time sufficient to form a colony from either the first cell population or the second cell population; and (d) subculturing a cell from the colony in a medium for a period time sufficient to prepare the ESC-like cell. | 09-09-2010 |
20100261270 | PATTERNED CELL SHEETS AND A METHOD FOR PRODUCTION OF THE SAME - The present invention is related to a method for the production of cell sheets comprising at least two different cell types, said method comprising the steps of providing a continuous cell sheet which is disposed on a substrate comprising shape transition properties and/or alterable surface characteristics; exposing said continuous cell sheet to a releasing agent in a patterned fashion; washing the cell sheet after exposure to the releasing agent in order to remove cells which have been affected by the releasing agent, and repopulating the gaps remaining after the cells which have been affected by the releasing agent have been removed with a second cell type. | 10-14-2010 |
20100311161 | METHOD FOR TISSUE REGENERATION - The invention relates to DNA-sequences, their use and the use of DNA-sequences of the MAG gene or genes encoding the high mobility group proteins, agents for the treatment of various diseases including tumors, influencing the development of the vascular system, as well as for contraception and tissue regeneration, and appropriate kits and processes. The sequences, agents, uses, kits and processes enable the specific influencing of molecular mechanisms that jointly form the basis for various diseases, the development of the vascular system, the contraception and the regeneration of tissue. Thus, the disadvantages associated with other agents or processes are decreased. | 12-09-2010 |
20110053266 | Methods, kits, and compositions for stem cell self-renewal - The present invention relates to methods and kits for expanding a stem cell population. More particularly, the invention relates, inter alia, to methods, kits, and compositions for expanding a stem cell population, particularly a hematopoietic stem cell population. | 03-03-2011 |
20110129917 | CELL ISOLATION METHOD - The present invention is a method for isolating a desired cell, which comprises selectively applying culture conditions including a culture medium to a sample potentially containing various cells, with addition of a cell enlarger simultaneously with, before or after the application. Thereby, it is possible to conveniently and efficiently obtain unknown but useful microorganisms occurring in a natural environment that are less competitive. | 06-02-2011 |
20110136226 | Stem cell bioprocessing and cell expansion - A stem cell niche for expanding stem cells in culture is described. The stem cell niche includes a scaffold, a plurality of stromal mesenchymal stem cells, and a plurality of umbilical cord blood stem cells grown in a rotating culture chamber. One embodiment of the rotating culture chamber has a fluid-filled compartment in which the umbilical cord blood stem cells are grown in the presence of the mesenchymal stem cells seeded on the scaffold. The culture chamber has a dual flow valving member at each end, wherein a first flow path passes under a molecular cut-off membrane covering a central core that transverses the culture chamber and a second flow path flows through the culture chamber and allows cells to be harvested while in suspension. | 06-09-2011 |
20110165673 | METHODS OF CULTURING BACTERIAL SPECIES OF THE ANAPLASMATACEAE FAMILY - The present invention relates to a method of culturing bacterial organisms belonging to the family Anaplasmataceae in mammalian embryonic or fetal cells. In particular, the present invention is directed to growth of bacterial organisms belonging to the family Anaplasmataceae including organisms belonging to the | 07-07-2011 |
20110250682 | FORMATION OF NEUROMUSCULAR JUNCTIONS IN A DEFINED SYSTEM - A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more rat muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a rat muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions. | 10-13-2011 |
20110263013 | Compositions And Methods For Growing Embryonic Stem Cells - Methods for deriving and cultivating human embryonic stem (ES) cells and maintaining their pluripotency in culture is provided by utilizing human umbilical cord blood derived stem cells or secreted proteins obtained from the culture medium of human umbilical cord blood derived stem cells. | 10-27-2011 |
20120040454 | PROCESS FOR PRODUCING A HUMAN T-CELL POPULATION HAVING BOTH CYTOTOXIC AND IMMUNOSUPPRESSIVE ACTIVITIES - The present invention has an object to provide a method for efficiently producing a human T cell population which has both cytotoxic and immunosuppressive activities, and solves the above object by providing a method for producing a human T cell population which has both cytotoxic and immunosuppressive activities, comprising the following steps (1) to (4): | 02-16-2012 |
20120115222 | COMPOSITION AND METHOD FOR MAINTENANCE, DIFFERENTIATION, AND PROLIFERATION OF STEM CELLS - Compositions and methods for the proliferation, differentiation, and maintenance of stem cells are described. Preferred are the use of hematopoietic stem cells in combination with a collagen matrix. | 05-10-2012 |
20120276624 | METHODS FOR IDENTIFYING FACTORS FOR DIFFERENTIATING DEFINITIVE ENDODERM - Disclosed herein are methods of identifying one or more differentiation factors that are useful for differentiating cells in a cell population comprising definitive endoderm cells into cells which are capable of forming tissues and/or organs that are derived from the gut tube. | 11-01-2012 |
20120322146 | NEURAL CELL POPULATIONS FROM PRIMATE PLURIPOTENT STEM CELLS - This invention provides a system for efficiently producing differentiated cells from pluripotent cells, such as human embryonic stem cells. Rather than permitting the cells to form embryoid bodies according to established techniques, differentiation is effected directly in monolayer culture on a suitable solid surface. The cells are either plated directly onto a differentiation-promoting surface, or grown initially on the solid surface in the absence of feeder cells and then exchanged into a medium that assists in the differentiation process. The solid surface and the culture medium can be chosen to direct differentiation down a particular pathway, generating a cell population that is remarkably uniform. The methodology is well adapted to bulk production of committed precursor and terminally differentiated cells for use in drug screening or regenerative medicine. | 12-20-2012 |
20130052728 | COMPOSITIONS AND METHODS FOR MAKING AND USING BONE MARROW MESENCHYMAL STEM CELLS AND ERYTHROID PROGENITOR CELLS - The invention provides compositions for making erythroid progenitor cells that comprise in vitro-activated bone marrow mesenchymal stem cells and embryoid bodies (EBs) or pluripotent stem cells, and methods for making and using them, including ameliorating (e.g., preventing or treating) anemia and/or stimulating erythropoiesis. In one embodiment, the invention provides methods of increasing propensity of committed stem cell differentiation towards the erythroid lineage. | 02-28-2013 |
20130115694 | FORMATION OF NEUROMUSCULAR JUNCTIONS IN A DEFINED SYSTEM - A method for forming neuromuscular junctions includes forming functional neuromuscular junctions between motoneurons and muscle cells by co-culturing one or more human motoneurons and one or more human muscle cells in a substantially serum-free medium. A synthetic mammalian neuromuscular junction includes a human motoneuron functionally linked to a human muscle cell in a substantially serum-free medium. An artificial substrate may be used to support the one or more neuromuscular junctions. | 05-09-2013 |
20130203161 | TECHNOLOGY AND METHOD TO STUDY MICROBIAL GROWTH AND ADHESION TO HOST-RELATED SURFACES AND THE HOST-MICROBIOTA INTERACTION - A method is provided for co-culturing viable cells and microorganisms for at least 48 hours in which an adhesion module is provided including a basal compartment and a luminal compartment separated by a semi-permeable membrane, and a continuous or semi-continuous flow of fresh medium is applied to the basal compartment. | 08-08-2013 |
20140045260 | METHODS AND COMPOSITIONS FOR PROMOTING SURVIVAL AND PROLIFERATION OF ENDOTHELIAL CELLS AND STIMULATING ANGIOGENESIS - The present invention relates to adenovirus E4ORF1 gene and to endothelial cells engineered to express the E4ORF1 gene. The present invention also relates to uses of the E4ORF1 gene, and cells expressing the E4ORF1 gene, and to compositions comprising the E4ORF1 gene, or comprising cells expressing the E4ORF1 gene. | 02-13-2014 |
20140057348 | Novel Culture System for Ex Vivo Development - The present invention provides methods for the culture of animal pluripotent stem cells and their differentiated progeny cells, tissues, and organs, and nonhuman animal embryos and fetuses. | 02-27-2014 |
20140099709 | ENGINEERED THREE-DIMENSIONAL CONNECTIVE TISSUE CONSTRUCTS AND METHODS OF MAKING THE SAME - Disclosed are engineered, living, three-dimensional connective tissue constructs comprising connective tissue cells. In some embodiments, the connective tissue cells are derived from multi-potent cells such as mesenchymal stem/stromal cells. In some embodiments, the cells are cohered to one another. In some embodiments, the multi-potent cells have been exposed to one or more differentiation signals to provide a living, three-dimensional connective tissue construct. In some embodiments, the constructs are substantially free of pre-formed scaffold at the time of use. Also disclosed are implants for engraftment, arrays of connective tissue constructs for in vitro experimentation, as well as methods of making the same. | 04-10-2014 |
20140127800 | Retinoic Acid Enhanced Human Stem Cell Derived Blood Brain Barrier Model - In one embodiment, the present invention is a method of creating a fully-human blood-brain barrier (BBB) model, comprising the steps of (a) obtaining a mixture of neural cells and brain microvascular endothelial cells (BMECs), wherein the neural cells and BMECs that comprise the mixture were produced from the differentiation of human pluripotent stem cells (hPSCs); (b) purifying BMECs from the mixture of neural cells and BMECs of step (a); and (c) co-culturing the purified BMECs with a cell type selected from the group consisting of pericytes, astrocytes and differentiated neural progenitor cells (NPCs), wherein a blood brain barrier model is created. | 05-08-2014 |
20140134721 | Chondrocyte Precursors Derived From Human Embryonic Stem Cells - This invention provides a system for obtaining cells of the chondrocyte lineage by differentiating primate pluripotent stem cells. The process involves culturing the cells as a micromass or other aggregate form in a cocktail of differentiation agents that facilitates outgrowth of the desired cell type. Progeny are capable of synthesizing Type II collagen or aggrecan, or other products that are characteristic of the chondrocyte lineage. Chondrocytes and chondrocyte precursor cells obtained according to this disclosure are suitable for use in both research and clinical therapy. | 05-15-2014 |
20140287495 | Methods of Inducing Differentiation of Stem Cells - The present invention relates to methods of inducing differentiation of stem cells. In particular, the invention relates to methods of inducing differentiation of embryonic stem cells into muscle cells or vascular endothelial cells. The invention also includes cells, cell lines, testing models and culture systems used in the methods of the present invention and differentiated cells produced therefrom. The present invention also provides methods of using the differentiated cells of the present invention for therapeutic purposes. | 09-25-2014 |
20140302599 | Method of Co-Culturing Mammalian Muscle Cells and Motoneurons - The invention provides a method of co-culturing mammalian muscle cells and mammalian motoneurons. The method comprises preparing one or more carriers coated with a covalently bonded monolayer of trimethoxysilylpropyl diethylenetriamine (DETA); suspending isolated fetal mammalian skeletal muscle cells in serum-free medium according to medium composition 1; suspending isolated fetal mammalian spinal motoneurons in serum-free medium according to medium composition 1; plating the suspended muscle cells onto the one or more carriers at a predetermined density and allowing the muscle cells to attach; plating the suspended motoneurons at a predetermined density onto the one or more carriers and allowing the motoneurons to attach; covering the one or more carriers with a mixture of medium composition 1 and medium composition 2; and incubating the carriers covered in the media mixture. | 10-09-2014 |
20140335610 | CELL CULTURE SUBSTRATE, AND METHOD FOR MANUFACTURING SAME - A cell culture substrate is used comprising a photopolymerization initiator immobilized on a surface of the cell culture substrate, and a linear polymer immobilized on a part or the entirety of the surface via the photopolymerization initiator, and wherein the photopolymerization initiator is thioxanthone. Thereby, advantageously, a single type or multiple types of cells are efficiently cultured on specific regions of the culture substrate, and efficiently detached only by changing temperature on the surface of the substrate. | 11-13-2014 |
20150050730 | Process for Ex Vivo Expansion of Stem Cells in a Bioreactor - The present invention refers to a process of ex vivo expansion of stem cells, in a bioreactor, in particular hematopoietic stem/progenitor cells co-cultured with mesenchymal stem cells immobilized on microcarriers, for transplantation. The process comprises the steps of: a) forming a suspension of mesenchymal stem cells immobilized on microcarriers, b) inoculating in a bioreactor containing an expansion medium, hematopoietic cells co-cultured with mesenchymal stem cells immobilized on microcarriers c) expansion of hematopoietic cells. The process of the invention is capable of being implemented in a Kit. | 02-19-2015 |
20150064781 | REPAIR AND REGENERATION OF OCULAR TISSUE USING POSTPARTUM-DERIVED CELLS - Cells derived from postpartum umbilicus and placenta are disclosed. Pharmaceutical compositions, devices and methods for the regeneration or repair of ocular tissue using the postpartum-derived cells are also disclosed. | 03-05-2015 |
20150072413 | CELL CULTURE APPARATUS AND CULTURE METHODS USING SAME - Cell culture apparatus comprising at least two adjacent cell cultivation channels separated by a permeable or semipermeable membrane, wherein at least one channel, for the majority of its length, has a cross sectional area of no more than 1 mm | 03-12-2015 |
20150079673 | HEPATOCYTES IN CO-CULTURE AND USES THEREOF - The present disclosure provides a human hepatocyte co-culture which maintains the phenotype of cells from human donors with diabetes, and methods of using same. The hepatocyte co-culture system provides an in vitro model in which both cell viability and phenotype are maintained for extended periods relative to primary human hepatocyte monocultures, and is used in methods for high throughput screening and evaluation of drug candidates, and kits for performing such testing. | 03-19-2015 |
20150079674 | METHODS OF GROWING AN EMBRYO TO A BLASTOCYST STAGE OF DEVELOPMENT - The present invention relates generally to the fields of reproductive medicine. More specifically, the present invention relates to a novel human embryo co-culture system to improve human embryo growth in vitro and, consequently, increase pregnancy rates in infertile women undergoing in vitro fertilization (IVF) treatment. More particularly, the present invention relates to a method of growing an embryo to a blastocyst stage of development comprising the step of coculturing said embryo in the presence of a population of cumulus cells. | 03-19-2015 |
20150132844 | Dendritic Cell Compositions and Methods - Methods are provided for the production of dendritic cells from monocytes that have been incubated at a temperature of 1° C.-34° C. for a period of approximately 6 to 96 hours from the time they are isolated from a subject. After the incubation period, the monocytes can then be induced to differentiate into dendritic cells. Mature dendritic cells made by the methods of the invention have increased levels of one or more of CD80, CD83, CD86, MHC class I molecules, or MHC class II molecules as compared to mature dendritic cells prepared from monocytes that have not been held at 1° C.-34° C. for at least 6 hours from the time they were isolated from a subject. Dendritic cells made by the methods of the invention are useful for the preparation of vaccines and for the stimulation of T cells. | 05-14-2015 |
20150147806 | METHOD FOR PREPARING TRANSFERRABLE NANOSCALE TRANSFERRABLE MEMBRANE AND USE THEREOF - Disclosed is a method for preparing a transferable membrane having a nanometer scale dimension in thickness and pore size by non-solvent vapor-induced phase separation process, comprising spin-casting a polymer solution in a closed humid chamber and controlling the relative humidity (RH) of the chamber using at least one supersaturated salts solution whereby the density of the pores are controlled. Also provided is a TNT membrane prepared by the present method and its use. The present membrane can be advantageously used as co-culture platform facilitating versatile and controllable cell co-culture assays and further allowing the quantitative analysis of paracrine communications between cells for example between cancer cells and different types of stromal cells by providing an in vivo-like environment, which can offer more in-vivo-like results to identify key signaling molecules for therapeutic targets of a disease. | 05-28-2015 |
20150353888 | METHOD FOR INDUCING ASTROCYTES - The present invention provides a method for producing astrocytes from neural progenitor cells, the method comprising: (1) culturing neural progenitor cells in a culture medium comprising a neurotrophic factor; (2) dissociating the cells obtained in the step (1); and (3) subjecting the cells obtained in the step (2) to adherent culture in a culture medium comprising a neurotrophic factor using an uncoated culture vessel. | 12-10-2015 |
20150368609 | PERIVASCULAR STROMAL CELLS FROM PRIMATE PLURIPOTENT STEM CELLS - The invention provides methods, compositions and kits for making and using pericyte-like cells or perivascular stromal cells derived from pPS cells. | 12-24-2015 |
20160040120 | In Vitro-Co-Culturesystem - The present invention relates to a three-dimensional (3D) model of a hematopoietic stem and progenitor cell (HSPC) niche, that comprises the coculture of human HSPC and human mesenchymal stromal cells in a defined 3D environment and thereby procures vital stem cell functions. | 02-11-2016 |
20160060593 | BLASTOID, CELL LINE BASED ARTIFICIAL BLASTOCYST - The invention relates to a method for making an at least double layered cell aggregate and/or an artificial blastocyst, and/or a further-developed blastoid termed blastoid, by forming a double layered cell aggregate from at least one trophoblast cell and at least one pluripotent and/or totipotent cell, and culturing said aggregate to obtain an artificial blastocyst. This artificial blastocyst has a trophectoderm-like tissue that surrounds a blastocoel and an inner cell mass-like tissue. The cell aggregate can be formed from toti- or pluripotent stem cell types, or induced pluripotent stem cell types, in combination with trophoblast stem cells. Formation of a blastoid can be achieved by culturing the cell aggregate in a medium preferably comprising one or more of a Rho/ROCK inhibitor, a Wnt pathway modulator, a PKA pathway modulator, a PKC pathway modulator, a MAPK pathway modulator, a STAT pathway modulator, an Akt pathway modulator, a Tgf pathway modulator and a Hippo pathway modulator. The invention further relates to a method for growing an at least double layered cell aggregate into an artificial blastocyst, and into a further-developed blastoid, a fetus or a live animal. The invention further pertains to an in vitro cell culture comprising the mentioned compounds and/or cell aggregates. | 03-03-2016 |
20160122722 | TISSUE STRUCTURE AND PREPARATION METHOD THEREOF - A tissue structure for enabling comprehensive understanding of gene patterns of mature cells and a method of preparing the tissue structure are provided. A tissue structure is obtained by co-culturing an endodermal, ectodermal, or mesodermal cell derived from a stem cell and at least one cell and/or factor selected from the group consisting of a vascular cell, a mesenchymal cell, a factor secreted by a vascular cell, a factor secreted by a mesenchymal cell, and a factor secreted when both a vascular cell and a mesenchymal cell exist. A value obtained by assay of a plurality of functions using a Pearson product-moment correlation coefficient is closer to a value of a cell or biological tissue sampled from an adult than a value of a cell or biological tissue sampled from a fetus. | 05-05-2016 |
20160136254 | METHOD OF MAKING A MYCOPLASMA VACCINE - The present invention relates to a method for the preparation of an immunogenic composition for the treatment and/or prophylaxis of mycoplasma infections in a subject comprising the cultivation of mycoplasma bacteria in a serum-reduced or swine serum-free, eukaryotic cell system; obtaining an antigen of the mycoplasma bacteria; and addition of a pharmaceutically acceptable carrier. Further, the present invention relates to the immunogenic composition obtainable by said method and a method for immunizing a subject comprising the administration of said immunogenic composition to a subject. | 05-19-2016 |
20160137977 | POLYMER SUITABLE FOR USE IN CELL CULTURE - A process for making an oligo(alkylene glycol) functionalized co-polyisocyanopeptide, wherein the process includes the steps of: i) copolymerizing a first comonomer of an oligo(alkylene glycol) functionalized isocyanopeptide grafted with a linking group and a second comonomer of a non-grafted oligo(alkylene glycol) functionalized isocyanopeptide, wherein the molar ratio between the first comonomer and the second comonomer is 1:500 and 1:30 and ii) adding a reactant of a spacer unit and a cell adhesion factor to the copolymer obtained by step i), wherein the spacer unit is represented by general formula A-L-B, wherein the linking group and group A are chosen to react and form a first coupling and the cell adhesion factor and group B are chosen to react and form a second coupling, wherein the first coupling and the second coupling are independently selected from the group consisting of alkyne-azide coupling, dibenzocyclooctyne-azide coupling, oxanorbornmadiene-based-azide couplings, vinylsulphone-thiol coupling, maleimide-thiol coupling, methyl methacrylate-thiol coupling, ether coupling, thioether coupling, biotin-strepavidin coupling, amine-carboxylic acid resulting in amides linkages, alcohol-carboxylic acid coupling resulting in esters linkages and NHS-Ester (N-Hydroxysuccinimide ester)-amine coupling and wherein group L is a linear chain segment having 10-60 bonds between atoms selected from C, N, O and S in the main chain. | 05-19-2016 |
20160145572 | Differentiated Pluripotent Stem Cell Progeny Depleted of Extraneous Phenotypes - The invention provides methods for depleting extraneous phenotypes from a mixed population of cells comprising the in vitro differentiated progeny of primate pluripotent stem cells. The invention also provides mixed cell populations enriched for a target cell phenotype where the mixed cell population comprises the differentiated in vitro progeny of primate embryonic stem cells. | 05-26-2016 |
20160251627 | Differentiation and Enrichment of Islet-like Cells From Human Pluripotent Stem Cells | 09-01-2016 |